Belongs to the ubiquitin-conjugating enzyme family
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Peripla_BP_6 | PF13458 | 45 - 337 | 1.7e-13 | Periplasmic binding protein |
| ANF_receptor | PF01094 | 49 - 411 | 1.6e-92 | Receptor family ligand binding region |
| SBP_bac_3 | PF00497 | 483 - 814 | 8.8e-13 | Bacterial extracellular solute-binding proteins, family 3 |
| Lig_chan-Glu_bd | PF10613 | 494 - 575 | 4.7e-09 | Ligated ion channel L-glutamate- and glycine-binding site |
| Lig_chan | PF00060 | 595 - 846 | 1.3e-35 | Ligand-gated ion channel |
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2219 |
| Acc36I | ACCTGC | 1 cut(s) | 2219 |
| Acc65I | GGTACC | 1 cut(s) | 1574 |
| AccB1I | GGYRCC | 1 cut(s) | 1574 |
| AccI | GTMKAC | 4 cut(s) | 468, 653, 2378, 2481 |
| AccII | CGCG | 3 cut(s) | 436, 971, 1392 |
| AciI | CCGC | 5 cut(s) | 164, 1392, 2382, 2485, 2526 |
| AclWI | GGATC | 5 cut(s) | 383, 670, 791, 2090, 2473 |
| AcoI | YGGCCR | 1 cut(s) | 294 |
| AcsI | RAATTY | 3 cut(s) | 884, 1054, 2116 |
| AcuI | CTGAAG | 3 cut(s) | 2148, 2474, 2654 |
| AfaI | GTAC | 8 cut(s) | 711, 857, 1339, 1352, 1545, 1576, 1992, 2582 |
| AfeI | AGCGCT | 1 cut(s) | 575 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 421, 1412, 1621, 2066, 2228, 2349 |
| AflIII | ACRYGT | 2 cut(s) | 969, 2373 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1886 |
| AhlI | ACTAGT | 1 cut(s) | 692 |
| AjnI | CCWGG | 1 cut(s) | 1366 |
| Alw21I | GWGCWC | 3 cut(s) | 357, 2274, 2615 |
| Alw26I | GTCTC | 1 cut(s) | 1759 |
| AlwI | GGATC | 5 cut(s) | 383, 670, 791, 2090, 2473 |
| Ama87I | CYCGRG | 1 cut(s) | 1457 |
| Aor51HI | AGCGCT | 1 cut(s) | 575 |
| AoxI | GGCC | 8 cut(s) | 294, 304, 961, 1166, 1364, 1868, 2205, 2509 |
| ApeKI | GCWGC | 5 cut(s) | 380, 576, 1517, 1947, 2240 |
| ApoI | RAATTY | 3 cut(s) | 884, 1054, 2116 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1103 |
| Asp718I | GGTACC | 1 cut(s) | 1574 |
| AspLEI | GCGC | 5 cut(s) | 576, 724, 1334, 1845, 2731 |
| AspS9I | GGNCC | 5 cut(s) | 305, 1166, 1868, 2219, 2509 |
| AsuHPI | GGTGA | 1 cut(s) | 566 |
| AsuII | TTCGAA | 1 cut(s) | 427 |
| AsuNHI | GCTAGC | 1 cut(s) | 562 |
| AvaI | CYCGRG | 1 cut(s) | 1457 |
| AvaII | GGWCC | 1 cut(s) | 2219 |
| BalI | TGGCCA | 1 cut(s) | 296 |
| BanI | GGYRCC | 1 cut(s) | 1574 |
| BanII | GRGCYC | 3 cut(s) | 357, 2274, 2615 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2472, 2504 |
| BbsI | GAAGAC | 1 cut(s) | 1885 |
| Bbv12I | GWGCWC | 3 cut(s) | 357, 2274, 2615 |
| BbvI | GCAGC | 5 cut(s) | 392, 563, 1504, 1959, 2227 |
| BccI | CCATC | 8 cut(s) | 733, 891, 1181, 1389, 1449, 1565, 1669, 2747 |
| BceAI | ACGGC | 1 cut(s) | 2667 |
| BciT130I | CCWGG | 1 cut(s) | 1368 |
| BclI | TGATCA | 2 cut(s) | 642, 2734 |
| BcoDI | GTCTC | 1 cut(s) | 1759 |
| BcuI | ACTAGT | 1 cut(s) | 692 |
| BfmI | CTRYAG | 3 cut(s) | 321, 1635, 2241 |
| BfoI | RGCGCY | 2 cut(s) | 577, 1335 |
| BfuAI | ACCTGC | 1 cut(s) | 2219 |
| BisI | GCNGC | 6 cut(s) | 381, 577, 1518, 1948, 2241, 2527 |
| BlsI | GCNGC | 6 cut(s) | 382, 578, 1519, 1949, 2242, 2528 |
| BmcAI | AGTACT | 3 cut(s) | 711, 1992, 2582 |
| Bme1390I | CCNGG | 1 cut(s) | 1368 |
| Bme18I | GGWCC | 1 cut(s) | 2219 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1886 |
| BmeT110I | CYCGRG | 1 cut(s) | 1457 |
| BmgT120I | GGNCC | 5 cut(s) | 305, 1166, 1868, 2219, 2509 |
| BmiI | GGNNCC | 5 cut(s) | 307, 1327, 1576, 1869, 2510 |
| BmrFI | CCNGG | 1 cut(s) | 1368 |
| BmsI | GCATC | 3 cut(s) | 1081, 1171, 1621 |
| BmtI | GCTAGC | 1 cut(s) | 566 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2635 |
| BpiI | GAAGAC | 1 cut(s) | 1885 |
| BpmI | CTGGAG | 3 cut(s) | 1275, 1312, 1990 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1424 |
| Bpu14I | TTCGAA | 1 cut(s) | 427 |
| BpuEI | CTTGAG | 2 cut(s) | 860, 981 |
| BsaBI | GATNNNNATC | 2 cut(s) | 674, 795 |
| BsaI | GGTCTC | 1 cut(s) | 1759 |
| BsaJI | CCNNGG | 3 cut(s) | 250, 549, 832 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 385, 415 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 421, 1412, 1621, 2066, 2228, 2349 |
| Bse118I | RCCGGY | 2 cut(s) | 1334, 2586 |
| Bse1I | ACTGG | 3 cut(s) | 1159, 1495, 1801 |
| Bse3DI | GCAATG | 1 cut(s) | 2529 |
| Bse8I | GATNNNNATC | 2 cut(s) | 674, 795 |
| BseBI | CCWGG | 1 cut(s) | 1368 |
| BseDI | CCNNGG | 3 cut(s) | 250, 549, 832 |
| BseGI | GGATG | 3 cut(s) | 828, 1400, 2767 |
| BseJI | GATNNNNATC | 2 cut(s) | 674, 795 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 421, 1412, 1621, 2066, 2228, 2349 |
| BseMI | GCAATG | 1 cut(s) | 2529 |
| BseMII | CTCAG | 5 cut(s) | 211, 330, 1371, 1438, 1754 |
| BseNI | ACTGG | 3 cut(s) | 1159, 1495, 1801 |
| BseXI | GCAGC | 5 cut(s) | 392, 563, 1504, 1959, 2227 |
| BsgI | GTGCAG | 5 cut(s) | 665, 1589, 1842, 2247, 2478 |
| Bsh1236I | CGCG | 3 cut(s) | 436, 971, 1392 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2382 |
| BshFI | GGCC | 8 cut(s) | 296, 306, 963, 1168, 1366, 1870, 2207, 2511 |
| BshNI | GGYRCC | 1 cut(s) | 1574 |
| BsiEI | CGRYCG | 1 cut(s) | 2382 |
| BsiHKAI | GWGCWC | 3 cut(s) | 357, 2274, 2615 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1457 |
| BsiSI | CCGG | 3 cut(s) | 1335, 2189, 2587 |
| BslFI | GGGAC | 4 cut(s) | 1252, 1421, 1544, 2366 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 421, 1412, 1621, 2066, 2228, 2349 |
| BsmAI | GTCTC | 1 cut(s) | 1759 |
| BsmFI | GGGAC | 4 cut(s) | 1252, 1421, 1544, 2366 |
| BsmI | GAATGC | 1 cut(s) | 2747 |
| BsnI | GGCC | 8 cut(s) | 296, 306, 963, 1168, 1366, 1870, 2207, 2511 |
| Bso31I | GGTCTC | 1 cut(s) | 1759 |
| BsoBI | CYCGRG | 1 cut(s) | 1457 |
| Bsp119I | TTCGAA | 1 cut(s) | 427 |
| Bsp1286I | GDGCHC | 3 cut(s) | 357, 2274, 2615 |
| Bsp1407I | TGTACA | 2 cut(s) | 855, 1350 |
| Bsp143I | GATC | 9 cut(s) | 388, 442, 642, 675, 796, 1359, 2095, 2465, 2734 |
| BspACI | CCGC | 5 cut(s) | 164, 1392, 2382, 2485, 2526 |
| BspANI | GGCC | 8 cut(s) | 296, 306, 963, 1168, 1366, 1870, 2207, 2511 |
| BspCNI | CTCAG | 5 cut(s) | 210, 329, 1372, 1437, 1755 |
| BspFNI | CGCG | 3 cut(s) | 436, 971, 1392 |
| BspHI | TCATGA | 1 cut(s) | 2425 |
| BspLI | GGNNCC | 5 cut(s) | 307, 1327, 1576, 1869, 2510 |
| BspMAI | CTGCAG | 1 cut(s) | 2245 |
| BspMI | ACCTGC | 1 cut(s) | 2219 |
| BspOI | GCTAGC | 1 cut(s) | 566 |
| BspPI | GGATC | 5 cut(s) | 383, 670, 791, 2090, 2473 |
| BspQI | GCTCTTC | 3 cut(s) | 2279, 2553, 2672 |
| BspT104I | TTCGAA | 1 cut(s) | 427 |
| BspT107I | GGYRCC | 1 cut(s) | 1574 |
| BspTNI | GGTCTC | 1 cut(s) | 1759 |
| BsrDI | GCAATG | 1 cut(s) | 2529 |
| BsrFI | RCCGGY | 2 cut(s) | 1334, 2586 |
| BsrGI | TGTACA | 2 cut(s) | 855, 1350 |
| BsrI | ACTGG | 3 cut(s) | 1159, 1495, 1801 |
| BssAI | RCCGGY | 2 cut(s) | 1334, 2586 |
| BssECI | CCNNGG | 3 cut(s) | 250, 549, 832 |
| BssMI | GATC | 9 cut(s) | 388, 442, 642, 675, 796, 1359, 2095, 2465, 2734 |
| BssT1I | CCWWGG | 2 cut(s) | 549, 832 |
| Bst2UI | CCWGG | 1 cut(s) | 1368 |
| Bst4CI | ACNGT | 5 cut(s) | 292, 1350, 1355, 2041, 2580 |
| Bst6I | CTCTTC | 3 cut(s) | 2279, 2553, 2672 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 149, 2456, 2535 |
| BstAUI | TGTACA | 2 cut(s) | 855, 1350 |
| BstBI | TTCGAA | 1 cut(s) | 427 |
| BstC8I | GCNNGC | 8 cut(s) | 564, 1118, 2020, 2259, 2452, 2536, 2592, 2615 |
| BstDEI | CTNAG | 6 cut(s) | 197, 316, 1380, 1424, 1763, 2522 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 2064, 2226, 2347 |
| BstF5I | GGATG | 3 cut(s) | 828, 1400, 2767 |
| BstFNI | CGCG | 3 cut(s) | 436, 971, 1392 |
| BstH2I | RGCGCY | 2 cut(s) | 577, 1335 |
| BstHHI | GCGC | 5 cut(s) | 576, 724, 1334, 1845, 2731 |
| BstKTI | GATC | 9 cut(s) | 391, 445, 645, 678, 799, 1362, 2098, 2468, 2737 |
| BstMAI | GTCTC | 1 cut(s) | 1759 |
| BstMBI | GATC | 9 cut(s) | 388, 442, 642, 675, 796, 1359, 2095, 2465, 2734 |
| BstMCI | CGRYCG | 1 cut(s) | 2382 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 149, 361, 2456, 2508, 2535 |
| BstNI | CCWGG | 1 cut(s) | 1368 |
| BstNSI | RCATGY | 4 cut(s) | 1315, 2377, 2454, 2594 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2635 |
| BstSCI | CCNGG | 1 cut(s) | 1366 |
| BstSFI | CTRYAG | 3 cut(s) | 321, 1635, 2241 |
| BstUI | CGCG | 3 cut(s) | 436, 971, 1392 |
| BstV1I | GCAGC | 5 cut(s) | 392, 563, 1504, 1959, 2227 |
| BstV2I | GAAGAC | 1 cut(s) | 1885 |
| BstX2I | RGATCY | 2 cut(s) | 388, 796 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1785 |
| BstYI | RGATCY | 2 cut(s) | 388, 796 |
| BsuRI | GGCC | 8 cut(s) | 296, 306, 963, 1168, 1366, 1870, 2207, 2511 |
| BtgZI | GCGATG | 1 cut(s) | 2766 |
| BtsCI | GGATG | 3 cut(s) | 828, 1400, 2767 |
| BtsI | GCAGTG | 5 cut(s) | 468, 2013, 2098, 2250, 2461 |
| BveI | ACCTGC | 1 cut(s) | 2219 |
| Cac8I | GCNNGC | 8 cut(s) | 564, 1118, 2020, 2259, 2452, 2536, 2592, 2615 |
| CciI | TCATGA | 1 cut(s) | 2425 |
| CfoI | GCGC | 5 cut(s) | 576, 724, 1334, 1845, 2731 |
| Cfr10I | RCCGGY | 2 cut(s) | 1334, 2586 |
| Cfr13I | GGNCC | 5 cut(s) | 305, 1166, 1868, 2219, 2509 |
| Csp6I | GTAC | 8 cut(s) | 710, 856, 1338, 1351, 1544, 1575, 1991, 2581 |
| CviQI | GTAC | 8 cut(s) | 710, 856, 1338, 1351, 1544, 1575, 1991, 2581 |
| DdeI | CTNAG | 6 cut(s) | 197, 316, 1380, 1424, 1763, 2522 |
| DpnI | GATC | 9 cut(s) | 390, 444, 644, 677, 798, 1361, 2097, 2467, 2736 |
| DpnII | GATC | 9 cut(s) | 388, 442, 642, 675, 796, 1359, 2095, 2465, 2734 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1886 |
| EaeI | YGGCCR | 1 cut(s) | 294 |
| Eam1104I | CTCTTC | 3 cut(s) | 2279, 2553, 2672 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1886 |
| EarI | CTCTTC | 3 cut(s) | 2279, 2553, 2672 |
| Ecl136II | GAGCTC | 3 cut(s) | 355, 2272, 2613 |
| Eco130I | CCWWGG | 2 cut(s) | 549, 832 |
| Eco147I | AGGCCT | 1 cut(s) | 2207 |
| Eco24I | GRGCYC | 3 cut(s) | 357, 2274, 2615 |
| Eco31I | GGTCTC | 1 cut(s) | 1759 |
| Eco47I | GGWCC | 1 cut(s) | 2219 |
| Eco47III | AGCGCT | 1 cut(s) | 575 |
| Eco53kI | GAGCTC | 3 cut(s) | 355, 2272, 2613 |
| Eco57I | CTGAAG | 3 cut(s) | 2148, 2474, 2654 |
| Eco88I | CYCGRG | 1 cut(s) | 1457 |
| EcoICRI | GAGCTC | 3 cut(s) | 355, 2272, 2613 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 2064, 2226, 2347 |
| EcoO109I | RGGNCCY | 3 cut(s) | 1868, 2219, 2509 |
| EcoRI | GAATTC | 2 cut(s) | 884, 2116 |
| EcoRII | CCWGG | 1 cut(s) | 1366 |
| EcoT14I | CCWWGG | 2 cut(s) | 549, 832 |
| EcoT38I | GRGCYC | 3 cut(s) | 357, 2274, 2615 |
| ErhI | CCWWGG | 2 cut(s) | 549, 832 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 2011, 2043, 2105, 2137 |
| FaqI | GGGAC | 4 cut(s) | 1252, 1421, 1544, 2366 |
| FauNDI | CATATG | 3 cut(s) | 958, 1537, 1976 |
| FbaI | TGATCA | 2 cut(s) | 642, 2734 |
| FblI | GTMKAC | 4 cut(s) | 468, 653, 2378, 2481 |
| Fnu4HI | GCNGC | 6 cut(s) | 381, 577, 1518, 1948, 2241, 2527 |
| FokI | GGATG | 2 cut(s) | 815, 1407 |
| FriOI | GRGCYC | 3 cut(s) | 357, 2274, 2615 |
| Fsp4HI | GCNGC | 6 cut(s) | 381, 577, 1518, 1948, 2241, 2527 |
| GlaI | GCGC | 5 cut(s) | 575, 723, 1333, 1844, 2730 |
| GluI | GCNGC | 6 cut(s) | 381, 577, 1518, 1948, 2241, 2527 |
| GsuI | CTGGAG | 3 cut(s) | 1275, 1312, 1990 |
| HaeII | RGCGCY | 2 cut(s) | 577, 1335 |
| HaeIII | GGCC | 8 cut(s) | 296, 306, 963, 1168, 1366, 1870, 2207, 2511 |
| HapII | CCGG | 3 cut(s) | 1335, 2189, 2587 |
| HhaI | GCGC | 5 cut(s) | 576, 724, 1334, 1845, 2731 |
| Hin6I | GCGC | 5 cut(s) | 574, 722, 1332, 1843, 2729 |
| HinP1I | GCGC | 5 cut(s) | 574, 722, 1332, 1843, 2729 |
| HincII | GTYRAC | 2 cut(s) | 1147, 2379 |
| HindII | GTYRAC | 2 cut(s) | 1147, 2379 |
| HindIII | AAGCTT | 3 cut(s) | 264, 2020, 2130 |
| HpaI | GTTAAC | 1 cut(s) | 1147 |
| HpaII | CCGG | 3 cut(s) | 1335, 2189, 2587 |
| HphI | GGTGA | 1 cut(s) | 566 |
| Hpy188III | TCNNGA | 9 cut(s) | 877, 894, 1088, 1292, 1426, 2312, 2426, 2608, 2738 |
| Hpy99I | CGWCG | 1 cut(s) | 2147 |
| HpyAV | CCTTC | 6 cut(s) | 810, 1610, 2197, 2456, 2498, 2691 |
| HpyCH4III | ACNGT | 5 cut(s) | 292, 1350, 1355, 2041, 2580 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 149, 361, 2456, 2508, 2535 |
| HpyF3I | CTNAG | 6 cut(s) | 197, 316, 1380, 1424, 1763, 2522 |
| HspAI | GCGC | 5 cut(s) | 574, 722, 1332, 1843, 2729 |
| KpnI | GGTACC | 1 cut(s) | 1578 |
| Ksp22I | TGATCA | 2 cut(s) | 642, 2734 |
| KspAI | GTTAAC | 1 cut(s) | 1147 |
| Kzo9I | GATC | 9 cut(s) | 388, 442, 642, 675, 796, 1359, 2095, 2465, 2734 |
| LguI | GCTCTTC | 3 cut(s) | 2279, 2553, 2672 |
| LmnI | GCTCC | 9 cut(s) | 360, 727, 900, 1305, 1331, 1729, 1840, 2044, 2269 |
| Lsp1109I | GCAGC | 5 cut(s) | 392, 563, 1504, 1959, 2227 |
| LweI | GCATC | 3 cut(s) | 1081, 1171, 1621 |
| MaeIII | GTNAC | 7 cut(s) | 73, 227, 242, 1493, 1530, 1792, 1888 |
| MalI | GATC | 9 cut(s) | 390, 444, 644, 677, 798, 1361, 2097, 2467, 2736 |
| MboI | GATC | 9 cut(s) | 388, 442, 642, 675, 796, 1359, 2095, 2465, 2734 |
| MfeI | CAATTG | 3 cut(s) | 1121, 2085, 2100 |
| MflI | RGATCY | 2 cut(s) | 388, 796 |
| MhlI | GDGCHC | 3 cut(s) | 357, 2274, 2615 |
| MlsI | TGGCCA | 1 cut(s) | 296 |
| MluI | ACGCGT | 1 cut(s) | 969 |
| MluNI | TGGCCA | 1 cut(s) | 296 |
| MlyI | GAGTC | 7 cut(s) | 54, 598, 671, 1443, 1455, 1712, 2348 |
| MmeI | TCCRAC | 2 cut(s) | 632, 2625 |
| Mox20I | TGGCCA | 1 cut(s) | 296 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1103 |
| MscI | TGGCCA | 1 cut(s) | 296 |
| MslI | CAYNNNNRTG | 3 cut(s) | 54, 1223, 2324 |
| Msp20I | TGGCCA | 1 cut(s) | 296 |
| MspI | CCGG | 3 cut(s) | 1335, 2189, 2587 |
| MspR9I | CCNGG | 1 cut(s) | 1368 |
| MunI | CAATTG | 3 cut(s) | 1121, 2085, 2100 |
| Mva1269I | GAATGC | 1 cut(s) | 2747 |
| MvaI | CCWGG | 1 cut(s) | 1368 |
| MvnI | CGCG | 3 cut(s) | 436, 971, 1392 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 149, 361, 2456, 2508, 2535 |
| NdeI | CATATG | 3 cut(s) | 958, 1537, 1976 |
| NdeII | GATC | 9 cut(s) | 388, 442, 642, 675, 796, 1359, 2095, 2465, 2734 |
| NheI | GCTAGC | 1 cut(s) | 562 |
| NlaIV | GGNNCC | 5 cut(s) | 307, 1327, 1576, 1869, 2510 |
| NmuCI | GTSAC | 3 cut(s) | 73, 242, 1888 |
| NspI | RCATGY | 4 cut(s) | 1315, 2377, 2454, 2594 |
| NspV | TTCGAA | 1 cut(s) | 427 |
| PaeI | GCATGC | 2 cut(s) | 2454, 2594 |
| PagI | TCATGA | 1 cut(s) | 2425 |
| PaqCI | CACCTGC | 1 cut(s) | 2219 |
| PceI | AGGCCT | 1 cut(s) | 2207 |
| PciI | ACATGT | 1 cut(s) | 2373 |
| PciSI | GCTCTTC | 3 cut(s) | 2279, 2553, 2672 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 433, 2572 |
| PctI | GAATGC | 1 cut(s) | 2747 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1103 |
| PkrI | GCNGC | 6 cut(s) | 382, 578, 1519, 1949, 2242, 2528 |
| PleI | GAGTC | 7 cut(s) | 54, 598, 670, 1442, 1454, 1711, 2348 |
| PpsI | GAGTC | 7 cut(s) | 54, 598, 670, 1442, 1454, 1711, 2348 |
| PpuMI | RGGWCCY | 1 cut(s) | 2219 |
| PscI | ACATGT | 1 cut(s) | 2373 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2635 |
| Psp124BI | GAGCTC | 3 cut(s) | 357, 2274, 2615 |
| Psp5II | RGGWCCY | 1 cut(s) | 2219 |
| Psp6I | CCWGG | 1 cut(s) | 1366 |
| PspGI | CCWGG | 1 cut(s) | 1366 |
| PspN4I | GGNNCC | 5 cut(s) | 307, 1327, 1576, 1869, 2510 |
| PspPI | GGNCC | 5 cut(s) | 305, 1166, 1868, 2219, 2509 |
| PspPPI | RGGWCCY | 1 cut(s) | 2219 |
| PstI | CTGCAG | 1 cut(s) | 2245 |
| PsuI | RGATCY | 2 cut(s) | 388, 796 |
| RsaI | GTAC | 8 cut(s) | 711, 857, 1339, 1352, 1545, 1576, 1992, 2582 |
| RsaNI | GTAC | 8 cut(s) | 710, 856, 1338, 1351, 1544, 1575, 1991, 2581 |
| RseI | CAYNNNNRTG | 3 cut(s) | 54, 1223, 2324 |
| SacI | GAGCTC | 3 cut(s) | 357, 2274, 2615 |
| SalI | GTCGAC | 1 cut(s) | 2377 |
| SapI | GCTCTTC | 3 cut(s) | 2279, 2553, 2672 |
| SatI | GCNGC | 6 cut(s) | 381, 577, 1518, 1948, 2241, 2527 |
| Sau3AI | GATC | 9 cut(s) | 388, 442, 642, 675, 796, 1359, 2095, 2465, 2734 |
| Sau96I | GGNCC | 5 cut(s) | 305, 1166, 1868, 2219, 2509 |
| ScaI | AGTACT | 3 cut(s) | 711, 1992, 2582 |
| SchI | GAGTC | 7 cut(s) | 54, 598, 671, 1443, 1455, 1712, 2348 |
| ScrFI | CCNGG | 1 cut(s) | 1368 |
| SduI | GDGCHC | 3 cut(s) | 357, 2274, 2615 |
| SfaNI | GCATC | 3 cut(s) | 1081, 1171, 1621 |
| SfcI | CTRYAG | 3 cut(s) | 321, 1635, 2241 |
| SfuI | TTCGAA | 1 cut(s) | 427 |
| SinI | GGWCC | 1 cut(s) | 2219 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 54, 1223, 2324 |
| SmlI | CTYRAG | 2 cut(s) | 875, 996 |
| SmoI | CTYRAG | 2 cut(s) | 875, 996 |
| SpeI | ACTAGT | 1 cut(s) | 692 |
| SphI | GCATGC | 2 cut(s) | 2454, 2594 |
| SseBI | AGGCCT | 1 cut(s) | 2207 |
| SsiI | CCGC | 5 cut(s) | 164, 1392, 2382, 2485, 2526 |
| SstI | GAGCTC | 3 cut(s) | 357, 2274, 2615 |
| StuI | AGGCCT | 1 cut(s) | 2207 |
| StyD4I | CCNGG | 1 cut(s) | 1366 |
| StyI | CCWWGG | 2 cut(s) | 549, 832 |
| TaaI | ACNGT | 5 cut(s) | 292, 1350, 1355, 2041, 2580 |
| TatI | WGTACW | 5 cut(s) | 709, 855, 1350, 1990, 2580 |
| TauI | GCSGC | 1 cut(s) | 2529 |
| TseFI | GTSAC | 3 cut(s) | 73, 242, 1888 |
| TseI | GCWGC | 5 cut(s) | 380, 576, 1517, 1947, 2240 |
| Tsp45I | GTSAC | 3 cut(s) | 73, 242, 1888 |
| TspDTI | ATGAA | 7 cut(s) | 17, 576, 1322, 1754, 2414, 2456, 2546 |
| TspGWI | ACGGA | 2 cut(s) | 896, 2421 |
| VpaK11BI | GGWCC | 1 cut(s) | 2219 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 2064, 2226, 2347 |
| XapI | RAATTY | 3 cut(s) | 884, 1054, 2116 |
| XceI | RCATGY | 4 cut(s) | 1315, 2377, 2454, 2594 |
| XmiI | GTMKAC | 4 cut(s) | 468, 653, 2378, 2481 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1103 |
| ZrmI | AGTACT | 3 cut(s) | 711, 1992, 2582 |