FvH4_3g33921

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002052
Physical Location & Seq
Forward (+)
29356027 .. 29357794
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g33921.t1

Sequence Viewer

Length: 1287 bp
ATGAGTTCTAGTGATGGGACACAAAAGAAAGATTTTGCTTGGCAATGGACTAAACCAATTCCTGGTCACTTGACATATTTTCTGTGTGCATTTTGTGATCTGAAAAATACCGGTGGCATCTTCCGATTCAAGCAGCATTTAGTTGGCACCCGCAAAGGAATTAGACCTTGTCCCAAGGTTCCACCAAATGTGAAAGATTTGTGTTCTCAAGCACTTCAAAGGAATGATAATCAGAAAAAAGCAAAGGTTGCCGTGCTTCGGGAGATAGGGGGATTAGAAAATTTAGAAGAAGAAGATGCACTAGATCATGAAGTGACAAATGTGACTACAACCCTTGCAAGTGATTGTGGTAGTGCTCAAACTCCTATTGTTGGTAGTTCTCAAAGTCCACTTGGTGGTGGTGGTTCTATGGGTCAACCAAAGGCTAGAGGACCGATGGACAACTATGTATCCTCAAAAGCTCGGCAAACTACGTTGAATACCTCTTACAAGAAACAAGAGAGGCGTAATGTGTGTAGGGCTATTGGGCGATTGTTTTATACAAGTGGATTGGCTTTCAATGTAGCAAATAATCCATACTATTTTGCGGCATTGGAGATGGTTTCCAAATATGGTCCGGGTTTTAAACCTCCTACAAGTCATGAGCTAAGGACTTGGATTTTGAAGGATGAAGTTGAAGATGTTCAAAAGTTGATGGTAGATCATAAAAAAGATTGGAGCAAGTATGGATGCACTATTATGTCGGATGGTTGGACGGATGGCAAGAGTAGGGTTATACTAAACTTTTTAGTAAATAGCCCTAAAGATGCTTATGATGTGAAGAAGTATATCCTCCGTGATGGAAACTTTTGGAACCATATTGCCTATTGCATCAAGAGTGTTTTGCCTCTTGTTTGTGTCTTGAGAGAGGTTGATTCAGAAGTGAGACCCGCCATGGGATTTATTTTTGAGTTGATAGATGCCGCAAAAGACAAGATTGCTAGTAATCTTGGAAATGTGGAAGCGAAGTATGCACCTATTTGGAAGAGAATAGATAATAGGTGGAGTCCACAACTTCATCAACCATTGCATGCGGCGGGTTATTACTTGAACCCTCAATTTCGATATGAAGAAAATTTTAAAAATACCGAGCATGTGAAGAAGGGTTTGGAAGATTGCATGGATAGGATGCTTGATGGTGAGGCTCGTATCCAAGCGGAAATACAATTGGACCTTTATGAGCGAAAACTTGGGAAGTTTGGAAGTGCAATGGCCGAATCTACTAGAAAAATCCGATCACCCGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

429

Amino Acids

48.29

Weight (kDa)

9.07

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 211 - 269 2.3e-18 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 146
AccB7I CCANNNNNTGG 2 cut(s) 62, 395
AciI CCGC 7 cut(s) 151, 587, 930, 963, 1073, 1076, 1196
AcoI YGGCCR 1 cut(s) 1251
AcsI RAATTY 2 cut(s) 280, 1114
AdeI CACNNNGTG 1 cut(s) 395
AfiI CCNNNNNNNGG 5 cut(s) 62, 258, 371, 395, 935
AgeI ACCGGT 1 cut(s) 110
AgsI TTSAA 8 cut(s) 130, 218, 478, 559, 664, 677, 686, 1090
AjnI CCWGG 1 cut(s) 61
AjuI GAANNNNNNNTTGG 2 cut(s) 1130, 1162
AluBI AGCT 2 cut(s) 461, 646
AluI AGCT 2 cut(s) 461, 646
Alw21I GWGCWC 1 cut(s) 358
Alw26I GTCTC 1 cut(s) 919
AoxI GGCC 1 cut(s) 1251
ApeKI GCWGC 1 cut(s) 133
ApoI RAATTY 2 cut(s) 280, 1114
ArsI GACNNNNNNTTYG 2 cut(s) 643, 675
AsiGI ACCGGT 1 cut(s) 110
Asp700I GAANNNNTTC 1 cut(s) 681
AspS9I GGNCC 3 cut(s) 431, 614, 1210
AsuC2I CCSGG 2 cut(s) 618, 1281
AsuHPI GGTGA 2 cut(s) 1190, 1269
AvaII GGWCC 3 cut(s) 431, 614, 1210
BanI GGYRCC 1 cut(s) 146
Bbv12I GWGCWC 1 cut(s) 358
BbvI GCAGC 1 cut(s) 145
BccI CCATC 8 cut(s) 8, 430, 592, 688, 740, 752, 833, 1169
BceAI ACGGC 1 cut(s) 236
BciT130I CCWGG 1 cut(s) 63
BciVI GTATCC 2 cut(s) 460, 1199
BcnI CCSGG 2 cut(s) 618, 1281
BcoDI GTCTC 1 cut(s) 919
BfaI CTAG 5 cut(s) 9, 302, 426, 981, 1263
BfuI GTATCC 2 cut(s) 460, 1199
BisI GCNGC 4 cut(s) 134, 588, 963, 1074
BlsI GCNGC 4 cut(s) 135, 589, 964, 1075
Bme1390I CCNGG 3 cut(s) 63, 618, 1281
Bme18I GGWCC 3 cut(s) 431, 614, 1210
BmgT120I GGNCC 3 cut(s) 431, 614, 1210
BmiI GGNNCC 3 cut(s) 148, 180, 854
BmrFI CCNGG 3 cut(s) 63, 618, 1281
BmsI GCATC 7 cut(s) 126, 286, 719, 796, 879, 949, 1158
Bpu10I CCTNAGC 1 cut(s) 647
BpuEI CTTGAG 2 cut(s) 192, 922
BpuMI CCSGG 2 cut(s) 618, 1281
BsaI GGTCTC 1 cut(s) 919
BsaJI CCNNGG 2 cut(s) 174, 933
BsaWI WCCGGW 1 cut(s) 110
Bsc4I CCNNNNNNNGG 5 cut(s) 62, 258, 371, 395, 935
Bse118I RCCGGY 1 cut(s) 110
Bse3DI GCAATG 3 cut(s) 50, 1064, 1254
BseBI CCWGG 1 cut(s) 63
BseDI CCNNGG 2 cut(s) 174, 933
BseGI GGATG 5 cut(s) 673, 734, 751, 763, 1173
BseLI CCNNNNNNNGG 5 cut(s) 62, 258, 371, 395, 935
BseMI GCAATG 3 cut(s) 50, 1064, 1254
BseXI GCAGC 1 cut(s) 145
BshFI GGCC 1 cut(s) 1253
BshNI GGYRCC 1 cut(s) 146
BshTI ACCGGT 1 cut(s) 110
BsiHKAI GWGCWC 1 cut(s) 358
BsiSI CCGG 3 cut(s) 111, 617, 1281
BslFI GGGAC 2 cut(s) 31, 156
BslI CCNNNNNNNGG 5 cut(s) 62, 258, 371, 395, 935
BsmAI GTCTC 1 cut(s) 919
BsmFI GGGAC 2 cut(s) 31, 156
BsnI GGCC 1 cut(s) 1253
Bso31I GGTCTC 1 cut(s) 919
Bsp1286I GDGCHC 1 cut(s) 358
Bsp143I GATC 4 cut(s) 97, 304, 700, 1274
Bsp19I CCATGG 1 cut(s) 933
BspACI CCGC 7 cut(s) 151, 587, 930, 963, 1073, 1076, 1196
BspANI GGCC 1 cut(s) 1253
BspHI TCATGA 2 cut(s) 307, 640
BspLI GGNNCC 3 cut(s) 148, 180, 854
BspT107I GGYRCC 1 cut(s) 146
BspTNI GGTCTC 1 cut(s) 919
BsrDI GCAATG 3 cut(s) 50, 1064, 1254
BsrFI RCCGGY 1 cut(s) 110
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 2 cut(s) 174, 933
BssMI GATC 4 cut(s) 97, 304, 700, 1274
BssT1I CCWWGG 2 cut(s) 174, 933
Bst2UI CCWGG 1 cut(s) 63
Bst6I CTCTTC 1 cut(s) 1019
BstAPI GCANNNNNTGC 1 cut(s) 248
BstC8I GCNNGC 1 cut(s) 1071
BstDEI CTNAG 1 cut(s) 647
BstDSI CCRYGG 1 cut(s) 933
BstF5I GGATG 5 cut(s) 673, 734, 751, 763, 1173
BstKTI GATC 4 cut(s) 100, 307, 703, 1277
BstMAI GTCTC 1 cut(s) 919
BstMBI GATC 4 cut(s) 97, 304, 700, 1274
BstMWI GCNNNNNNNGC 2 cut(s) 248, 1010
BstNI CCWGG 1 cut(s) 63
BstNSI RCATGY 2 cut(s) 1073, 1136
BstSCI CCNGG 3 cut(s) 61, 616, 1279
BstV1I GCAGC 1 cut(s) 145
BsuI GTATCC 2 cut(s) 460, 1199
BsuRI GGCC 1 cut(s) 1253
BtgI CCRYGG 1 cut(s) 933
BtsCI GGATG 5 cut(s) 673, 734, 751, 763, 1173
Cac8I GCNNGC 1 cut(s) 1071
CciI TCATGA 2 cut(s) 307, 640
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 3 cut(s) 431, 614, 1210
CspAI ACCGGT 1 cut(s) 110
CviAII CATG 6 cut(s) 308, 641, 934, 1070, 1133, 1159
CviJI RGCY 8 cut(s) 425, 461, 521, 554, 646, 798, 1184, 1253
CviKI_1 RGCY 8 cut(s) 425, 461, 521, 554, 646, 798, 1184, 1253
DdeI CTNAG 1 cut(s) 647
DpnI GATC 4 cut(s) 99, 306, 702, 1276
DpnII GATC 4 cut(s) 97, 304, 700, 1274
DraI TTTAAA 2 cut(s) 625, 1120
DraIII CACNNNGTG 1 cut(s) 395
EaeI YGGCCR 1 cut(s) 1251
Eam1104I CTCTTC 1 cut(s) 1019
EarI CTCTTC 1 cut(s) 1019
Eco130I CCWWGG 2 cut(s) 174, 933
Eco31I GGTCTC 1 cut(s) 919
Eco47I GGWCC 3 cut(s) 431, 614, 1210
EcoRII CCWGG 1 cut(s) 61
EcoT14I CCWWGG 2 cut(s) 174, 933
ErhI CCWWGG 2 cut(s) 174, 933
FaeI CATG 6 cut(s) 311, 644, 937, 1073, 1136, 1162
FaqI GGGAC 2 cut(s) 31, 156
FatI CATG 6 cut(s) 307, 640, 933, 1069, 1132, 1158
FauI CCCGC 3 cut(s) 158, 937, 1069
Fnu4HI GCNGC 4 cut(s) 134, 588, 963, 1074
FokI GGATG 5 cut(s) 680, 741, 758, 770, 1180
Fsp4HI GCNGC 4 cut(s) 134, 588, 963, 1074
FspBI CTAG 5 cut(s) 9, 302, 426, 981, 1263
GluI GCNGC 4 cut(s) 134, 588, 963, 1074
HaeIII GGCC 1 cut(s) 1253
HapII CCGG 3 cut(s) 111, 617, 1281
Hin1II CATG 6 cut(s) 311, 644, 937, 1073, 1136, 1162
HincII GTYRAC 1 cut(s) 416
HindII GTYRAC 1 cut(s) 416
HinfI GANTC 4 cut(s) 126, 914, 1045, 1256
HpaII CCGG 3 cut(s) 111, 617, 1281
HphI GGTGA 2 cut(s) 1190, 1269
Hpy166II GTNNAC 3 cut(s) 389, 416, 1049
Hpy188I TCNGA 6 cut(s) 102, 125, 234, 745, 919, 1274
Hpy188III TCNNGA 5 cut(s) 260, 308, 641, 874, 901
Hpy8I GTNNAC 3 cut(s) 389, 416, 1049
HpyAV CCTTC 2 cut(s) 658, 1135
HpyCH4IV ACGT 1 cut(s) 473
HpyCH4V TGCA 9 cut(s) 89, 299, 338, 732, 870, 1013, 1069, 1158, 1247
HpyF10VI GCNNNNNNNGC 2 cut(s) 248, 1010
HpyF3I CTNAG 1 cut(s) 647
HpySE526I ACGT 1 cut(s) 473
Hsp92II CATG 6 cut(s) 311, 644, 937, 1073, 1136, 1162
Kzo9I GATC 4 cut(s) 97, 304, 700, 1274
LmnI GCTCC 1 cut(s) 717
LpnPI CCDG 4 cut(s) 48, 75, 124, 630
Lsp1109I GCAGC 1 cut(s) 145
LweI GCATC 7 cut(s) 126, 286, 719, 796, 879, 949, 1158
MaeI CTAG 5 cut(s) 9, 302, 426, 981, 1263
MaeII ACGT 1 cut(s) 473
MaeIII GTNAC 3 cut(s) 65, 313, 322
MalI GATC 4 cut(s) 99, 306, 702, 1276
MboI GATC 4 cut(s) 97, 304, 700, 1274
MfeI CAATTG 1 cut(s) 1205
MhlI GDGCHC 1 cut(s) 358
MluCI AATT 6 cut(s) 57, 159, 280, 1097, 1114, 1205
MlyI GAGTC 1 cut(s) 1054
MmeI TCCRAC 2 cut(s) 723, 731
MroXI GAANNNNTTC 1 cut(s) 681
MseI TTAA 2 cut(s) 624, 1119
MslI CAYNNNNRTG 1 cut(s) 737
MspI CCGG 3 cut(s) 111, 617, 1281
MspR9I CCNGG 3 cut(s) 63, 618, 1281
MunI CAATTG 1 cut(s) 1205
MvaI CCWGG 1 cut(s) 63
MwoI GCNNNNNNNGC 2 cut(s) 248, 1010
NciI CCSGG 2 cut(s) 618, 1281
NcoI CCATGG 1 cut(s) 933
NdeII GATC 4 cut(s) 97, 304, 700, 1274
NlaIII CATG 6 cut(s) 311, 644, 937, 1073, 1136, 1162
NlaIV GGNNCC 3 cut(s) 148, 180, 854
NmeAIII GCCGAG 1 cut(s) 442
NmuCI GTSAC 3 cut(s) 65, 313, 322
NspI RCATGY 2 cut(s) 1073, 1136
PaeI GCATGC 1 cut(s) 1073
PagI TCATGA 2 cut(s) 307, 640
PdmI GAANNNNTTC 1 cut(s) 681
PfeI GAWTC 3 cut(s) 126, 914, 1256
PflFI GACNNNGTC 1 cut(s) 168
PflMI CCANNNNNTGG 2 cut(s) 62, 395
PinAI ACCGGT 1 cut(s) 110
PkrI GCNGC 4 cut(s) 135, 589, 964, 1075
PleI GAGTC 1 cut(s) 1053
PpsI GAGTC 1 cut(s) 1053
Psp6I CCWGG 1 cut(s) 61
PspGI CCWGG 1 cut(s) 61
PspN4I GGNNCC 3 cut(s) 148, 180, 854
PspPI GGNCC 3 cut(s) 431, 614, 1210
PsyI GACNNNGTC 1 cut(s) 168
RseI CAYNNNNRTG 1 cut(s) 737
SaqAI TTAA 2 cut(s) 624, 1119
SatI GCNGC 4 cut(s) 134, 588, 963, 1074
Sau3AI GATC 4 cut(s) 97, 304, 700, 1274
Sau96I GGNCC 3 cut(s) 431, 614, 1210
SchI GAGTC 1 cut(s) 1054
ScrFI CCNGG 3 cut(s) 63, 618, 1281
SduI GDGCHC 1 cut(s) 358
SfaNI GCATC 7 cut(s) 126, 286, 719, 796, 879, 949, 1158
SinI GGWCC 3 cut(s) 431, 614, 1210
SmiMI CAYNNNNRTG 1 cut(s) 737
SmlI CTYRAG 2 cut(s) 207, 901
SmoI CTYRAG 2 cut(s) 207, 901
SphI GCATGC 1 cut(s) 1073
Sse9I AATT 6 cut(s) 57, 159, 280, 1097, 1114, 1205
SsiI CCGC 7 cut(s) 151, 587, 930, 963, 1073, 1076, 1196
SspMI CTAG 5 cut(s) 9, 302, 426, 981, 1263
StyD4I CCNGG 3 cut(s) 61, 616, 1279
StyI CCWWGG 2 cut(s) 174, 933
TaiI ACGT 1 cut(s) 476
TaqI TCGA 1 cut(s) 1102
TaqII GACCGA 1 cut(s) 448
TasI AATT 6 cut(s) 57, 159, 280, 1097, 1114, 1205
TauI GCSGC 3 cut(s) 590, 965, 1076
TfiI GAWTC 3 cut(s) 126, 914, 1256
Tru1I TTAA 2 cut(s) 624, 1119
Tru9I TTAA 2 cut(s) 624, 1119
TseFI GTSAC 3 cut(s) 65, 313, 322
TseI GCWGC 1 cut(s) 133
Tsp45I GTSAC 3 cut(s) 65, 313, 322
TspDTI ATGAA 4 cut(s) 324, 684, 1046, 1122
TspGWI ACGGA 2 cut(s) 770, 824
Tth111I GACNNNGTC 1 cut(s) 168
Van91I CCANNNNNTGG 2 cut(s) 62, 395
VpaK11BI GGWCC 3 cut(s) 431, 614, 1210
XapI RAATTY 2 cut(s) 280, 1114
XceI RCATGY 2 cut(s) 1073, 1136
XmnI GAANNNNTTC 1 cut(s) 681
XspI CTAG 5 cut(s) 9, 302, 426, 981, 1263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.