Rorug03G0268900

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002052
Physical Location & Seq
Forward (+)
26424533 .. 26435020
10488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0268900.1

Sequence Viewer

Length: 2256 bp
ATGGCACAGTGTCGCTTCTCCTCCTCGCTTCTTCTTCGTCTCCGTCTCCGTTCAGCTCGCCGGTTCTCCTCACTCCCGAACCCAACAACAACCCAAAACGACGCCGAACTGGTATCAAACATCCTCCTCCACCACCACAACCCCTTCCACGCCATGGAGTCCTCTCTCCAGCTCCACGGCATCACTCTCTCCCCCGACCTCCTCCACCACACTCTCCTCCGCCTCAAGCACAATTCCAAAATCGCCCTCGCCCTCTTCCACTACGCCAAGTCCCTCCCCTCCCCCCCGCCTCTCACCTCCCACGCCTTCCACCTCCTCATCGACATCGTCGCCAAGGTCCGCCAGTACGACGTCGCTTGGCAGCTCATCCTCGAAATGGACCGCCACAACCTCACCCCCACCGCCGCCACCTTCCTCATCCTCGTCCGCCGCCTCATCGCCTCCGGCCTCACCCGCCAGGCCGTCCGGGCCTTCGACGACATCGAGACCTTCGTCCTGACCCGCCCCACCGCCCAAGACTTCTGCTTCTTACTCGACACCCTCTGTAAATACGGCTACGTTAAGCTCGCGGCGGAAGTTTTCAACAAAAGGAAGCATGACTATGTGGCCGATGTGAAAATGTACACTGTTCTGGTCTACGGGTGGTGTAAGATCGGCCGTTTCGATATGGCGGAGAGGTTCTTGAGGGATATGATCGAGCGAGGTATTGAGCCCAATGTGGTTACTTACAATGTGTTTCTGGATGGGATTTGTAGGAAGGCGAGTTTGCACCCTCAGGACAGGTTTGAGAGGACCATAAGAAATGCACTGAAGGTGTTCGATGAAATGTCCAGCAGAGGCATTGAGCCGGACGTGACTAGCTTTTCGATCCTTCTTCATGTTTATAGCCGGGCGCATAAGCCCGAGTTGTCGCTTGATAAGCTGAAGGAAATGAGGGAGAGGGGGATTTGCCCCACCGTGGCGACTTACACTTCAGTTGTCAAGTGTCTCTGCTCTTGCGGAAGGCTTGAGGAGGCGGAGGAGTTGCTTGGTGAGATGGTGAGGAGTGGGGTTAGTCCCTCGGCGGCTACTTATAATTGTTTTTTCAAAGAGTATAGGGGGAGGAAGGACTCGGAAAGCGCTTTGAAGTTGTATAGGAAGATGAAGGAGGATGGATTGTTTGCGCCGAGTGTGCACACTTATAATATATTGGTGGGGATGTTTTTGCAGTTGAATCGAGTGGAGATTGTGAGAGAGATATGGGATGACATGAAAGAGAGCGGGGCGGGGCCAGATTTGGATTCGTATACAATGTTGATTCATGGGCTATGTGAGAAGCAGAAGTGGAGAGAGGCGTGCCAGTTTTTTGTGGAGATGATAGAGAAGGGGCTTCTTCCTCAGAAAGTTACTTTTGAGACGCTTTACAGAGGGTTAATACAGTCTGATATGTTGAGAACTTGGAGAAGATTGAAGAAGAAGCTCGATGAAGAGTCTTGGATTATAGAATCTGTTGTCAAGTTACTTGCTGCTTCTATGGTTCACATAAAGGAAAACATTAACGATAACCTTCTTGCTTCAGACCATAACAGAGATCTGGATGCAGTTAGTGTCTTTAGGAAAGTCATGACAGGTTGTCTGAGGATCTTCTTCAAAAGAAGTTACCTGAGACGAGGATTTCAAGGACACTCAACAATTGCCCAGATATGTGATGGATGGCTACAGTATATATGCATATTTTCTTATGTCAAACCGGATCTAGCAAAACAGATACCATGCATGCAGGCATTTGTCATATATTGCATGAAAGTGGTTCCATCAGTGAATTCAACCACTGATATTCTTATCCTCATGAAATATGGTGAGGTGAATCGAGTTGTCAGTTCTACTGCAATCAATAACTGCAGTAGCCATTCCCAGAGAAGCTCAGTTGATGGCTTAATGAGTATTGATTTCAAAGATGGAGTTCTCATGATTCCACAGCTTGCAATTGCAGAGATGAATGAACCTTTGTTCAGGAACCTCATAGCCTTCGAGCAATGCTATAATGGTCGCGGGCATAAAGCAACTTCTTATGCACTTTTAATGGATAACCTCATTGCTTCCAGCAAGGATGTTGTTTTTCTTTGTGACAGAGAAATAATCCATAATTGGTTGAGTGCTGAAGAAGCGTCCCAGTTGTTCAGTAAGCTTTATAGTGACACATTGATTGATGATTTTTGCTATGGCGGGCTCTGTGCTACAGTGAATAAATGTTACAAAGATGGAAAAAGTGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

751

Amino Acids

85.89

Weight (kDa)

8.59

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 204 - 252 1.4e-13 PPR repeat family
PPR_long PF17177 230 - 359 4.1e-11 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 266 - 295 8.9e-07 PPR repeat family
PPR_3 PF13812 271 - 328 1.9e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 288 - 331 1.5e-07 PPR repeat family
TPR_24 PF23276 292 - 407 3.5e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 314 - 347 5.4e-11 PPR repeat
PPR_2 PF13041 322 - 364 1.3e-10 PPR repeat family
PPR PF01535 322 - 351 3.8e-08 PPR repeat
PPR_3 PF13812 342 - 401 5.9e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 357 - 401 1.4e-07 PPR repeat family
PPR_2 PF13041 389 - 437 1.6e-11 PPR repeat family
PPR_2 PF13041 424 - 471 4.7e-10 PPR repeat family
PPR_1 PF12854 424 - 452 1.1e-06 PPR repeat
DUF247 PF03140 633 - 747 1.7e-28 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1074, 1182
AatII GACGTC 1 cut(s) 354
AccB7I CCANNNNNTGG 1 cut(s) 154
AccBSI CCGCTC 1 cut(s) 1260
AccI GTMKAC 2 cut(s) 636, 1286
AccII CGCG 2 cut(s) 569, 2031
AclWI GGATC 3 cut(s) 862, 1628, 1740
AcoI YGGCCR 2 cut(s) 606, 655
AcsI RAATTY 1 cut(s) 1801
AcuI CTGAAG 5 cut(s) 830, 944, 957, 1539, 2160
AcyI GRCGYC 2 cut(s) 102, 351
AfaI GTAC 2 cut(s) 347, 623
AfeI AGCGCT 1 cut(s) 1120
AfiI CCNNNNNNNGG 3 cut(s) 154, 376, 958
AgsI TTSAA 9 cut(s) 583, 1087, 1126, 1213, 1450, 1630, 1658, 1806, 1933
AhdI GACNNNNNGTC 1 cut(s) 1611
AjiI CACGTC 1 cut(s) 853
AjnI CCWGG 1 cut(s) 456
Alw21I GWGCWC 1 cut(s) 1176
Alw26I GTCTC 6 cut(s) 44, 50, 479, 992, 1388, 1639
Alw44I GTGCAC 1 cut(s) 1172
AlwI GGATC 3 cut(s) 862, 1628, 1740
Ama87I CYCGRG 1 cut(s) 902
Aor51HI AGCGCT 1 cut(s) 1120
AoxI GGCC 6 cut(s) 445, 459, 468, 606, 655, 1268
ApaLI GTGCAC 1 cut(s) 1172
ApeKI GCWGC 2 cut(s) 361, 1505
ApoI RAATTY 1 cut(s) 1801
ArsI GACNNNNNNTTYG 2 cut(s) 847, 879
Asp700I GAANNNNTTC 1 cut(s) 815
AspLEI GCGC 3 cut(s) 895, 1121, 1165
AspS9I GGNCC 5 cut(s) 337, 379, 468, 792, 1268
AsuC2I CCSGG 2 cut(s) 467, 890
AsuHPI GGTGA 7 cut(s) 286, 385, 442, 1043, 1051, 1850, 1855
AvaI CYCGRG 1 cut(s) 902
AvaII GGWCC 3 cut(s) 337, 379, 792
AxyI CCTNAGG 1 cut(s) 774
BaeGI GKGCMC 1 cut(s) 1176
BanII GRGCYC 2 cut(s) 714, 2211
Bbv12I GWGCWC 1 cut(s) 1176
BbvI GCAGC 2 cut(s) 373, 1492
BccI CCATC 9 cut(s) 737, 1030, 1145, 1682, 1686, 1801, 1904, 1931, 2234
BceAI ACGGC 4 cut(s) 193, 446, 568, 642
BcgI CGANNNNNNTGC 2 cut(s) 1196, 1230
BciT130I CCWGG 1 cut(s) 458
BcnI CCSGG 2 cut(s) 467, 890
BcoDI GTCTC 6 cut(s) 44, 50, 479, 992, 1388, 1639
BfaI CTAG 3 cut(s) 858, 1736, 2254
BfmI CTRYAG 3 cut(s) 1697, 1879, 2217
BfoI RGCGCY 1 cut(s) 1122
BglI GCCNNNNNGGC 1 cut(s) 467
BglII AGATCT 1 cut(s) 1570
BisI GCNGC 6 cut(s) 362, 405, 430, 570, 1065, 1506
BlsI GCNGC 6 cut(s) 363, 406, 431, 571, 1066, 1507
Bme1390I CCNGG 3 cut(s) 458, 467, 890
Bme18I GGWCC 3 cut(s) 337, 379, 792
BmeRI GACNNNNNGTC 1 cut(s) 1611
BmeT110I CYCGRG 1 cut(s) 902
BmgBI CACGTC 1 cut(s) 853
BmgT120I GGNCC 5 cut(s) 337, 379, 468, 792, 1268
BmiI GGNNCC 3 cut(s) 1269, 1791, 1997
BmrFI CCNGG 3 cut(s) 458, 467, 890
BmrI ACTGGG 1 cut(s) 2146
BmsI GCATC 2 cut(s) 189, 1567
BmuI ACTGGG 1 cut(s) 2146
BoxI GACNNNNGTC 1 cut(s) 491
BpmI CTGGAG 1 cut(s) 152
BpuEI CTTGAG 3 cut(s) 209, 703, 1028
BpuMI CCSGG 2 cut(s) 467, 890
BsaHI GRCGYC 2 cut(s) 102, 351
BsaI GGTCTC 1 cut(s) 479
BsaJI CCNNGG 5 cut(s) 153, 175, 333, 957, 1059
BsaWI WCCGGW 1 cut(s) 1729
Bsc4I CCNNNNNNNGG 3 cut(s) 154, 376, 958
Bse118I RCCGGY 1 cut(s) 60
Bse1I ACTGG 4 cut(s) 114, 343, 1339, 2152
Bse21I CCTNAGG 1 cut(s) 774
Bse3DI GCAATG 2 cut(s) 2021, 2073
BseBI CCWGG 1 cut(s) 458
BseDI CCNNGG 5 cut(s) 153, 175, 333, 957, 1059
BseLI CCNNNNNNNGG 3 cut(s) 154, 376, 958
BseMI GCAATG 2 cut(s) 2021, 2073
BseMII CTCAG 5 cut(s) 788, 1391, 1607, 1634, 1917
BseNI ACTGG 4 cut(s) 114, 343, 1339, 2152
BseSI GKGCMC 1 cut(s) 1176
BseX3I CGGCCG 1 cut(s) 655
BseXI GCAGC 2 cut(s) 373, 1492
Bsh1236I CGCG 2 cut(s) 569, 2031
Bsh1285I CGRYCG 1 cut(s) 658
BshFI GGCC 6 cut(s) 447, 461, 470, 608, 657, 1270
BsiEI CGRYCG 1 cut(s) 658
BsiHKAI GWGCWC 1 cut(s) 1176
BsiHKCI CYCGRG 1 cut(s) 902
BsiSI CCGG 6 cut(s) 61, 444, 466, 848, 889, 1730
BslFI GGGAC 3 cut(s) 256, 1041, 2134
BslI CCNNNNNNNGG 3 cut(s) 154, 376, 958
BsmAI GTCTC 6 cut(s) 44, 50, 479, 992, 1388, 1639
BsmBI CGTCTC 4 cut(s) 44, 50, 1388, 1639
BsmFI GGGAC 3 cut(s) 256, 1041, 2134
BsnI GGCC 6 cut(s) 447, 461, 470, 608, 657, 1270
Bso31I GGTCTC 1 cut(s) 479
BsoBI CYCGRG 1 cut(s) 902
Bsp1286I GDGCHC 3 cut(s) 714, 1176, 2211
Bsp1407I TGTACA 1 cut(s) 621
Bsp143I GATC 6 cut(s) 651, 693, 867, 1570, 1620, 1732
Bsp19I CCATGG 1 cut(s) 153
BspANI GGCC 6 cut(s) 447, 461, 470, 608, 657, 1270
BspCNI CTCAG 5 cut(s) 787, 1390, 1608, 1635, 1916
BspFNI CGCG 2 cut(s) 569, 2031
BspHI TCATGA 3 cut(s) 1602, 1827, 1947
BspLI GGNNCC 3 cut(s) 1269, 1791, 1997
BspMAI CTGCAG 1 cut(s) 1883
BspPI GGATC 3 cut(s) 862, 1628, 1740
BspTNI GGTCTC 1 cut(s) 479
BsrBI CCGCTC 1 cut(s) 1260
BsrDI GCAATG 2 cut(s) 2021, 2073
BsrFI RCCGGY 1 cut(s) 60
BsrGI TGTACA 1 cut(s) 621
BsrI ACTGG 4 cut(s) 114, 343, 1339, 2152
BssAI RCCGGY 1 cut(s) 60
BssECI CCNNGG 5 cut(s) 153, 175, 333, 957, 1059
BssMI GATC 6 cut(s) 651, 693, 867, 1570, 1620, 1732
BssNAI GTATAC 1 cut(s) 1287
BssNI GRCGYC 2 cut(s) 102, 351
BssT1I CCWWGG 2 cut(s) 153, 333
Bst1107I GTATAC 1 cut(s) 1287
Bst2UI CCWGG 1 cut(s) 458
Bst4CI ACNGT 6 cut(s) 9, 628, 958, 1419, 1701, 2221
Bst6I CTCTTC 2 cut(s) 260, 1461
BstACI GRCGYC 2 cut(s) 102, 351
BstAUI TGTACA 1 cut(s) 621
BstC8I GCNNGC 8 cut(s) 58, 567, 1336, 1757, 1761, 1962, 2033, 2207
BstDEI CTNAG 5 cut(s) 774, 1377, 1616, 1643, 1903
BstDSI CCRYGG 3 cut(s) 153, 175, 957
BstFNI CGCG 2 cut(s) 569, 2031
BstH2I RGCGCY 1 cut(s) 1122
BstHHI GCGC 3 cut(s) 895, 1121, 1165
BstKTI GATC 6 cut(s) 654, 696, 870, 1573, 1623, 1735
BstMAI GTCTC 6 cut(s) 44, 50, 479, 992, 1388, 1639
BstMBI GATC 6 cut(s) 651, 693, 867, 1570, 1620, 1732
BstMCI CGRYCG 1 cut(s) 658
BstMWI GCNNNNNNNGC 5 cut(s) 453, 467, 919, 1171, 2144
BstNI CCWGG 1 cut(s) 458
BstNSI RCATGY 1 cut(s) 1759
BstPAI GACNNNNGTC 1 cut(s) 491
BstSCI CCNGG 3 cut(s) 456, 465, 888
BstSFI CTRYAG 3 cut(s) 1697, 1879, 2217
BstSLI GKGCMC 1 cut(s) 1176
BstUI CGCG 2 cut(s) 569, 2031
BstV1I GCAGC 2 cut(s) 373, 1492
BstX2I RGATCY 3 cut(s) 1570, 1620, 1732
BstYI RGATCY 3 cut(s) 1570, 1620, 1732
BstZ17I GTATAC 1 cut(s) 1287
BstZI CGGCCG 1 cut(s) 655
Bsu36I CCTNAGG 1 cut(s) 774
BsuRI GGCC 6 cut(s) 447, 461, 470, 608, 657, 1270
BtgI CCRYGG 3 cut(s) 153, 175, 957
BtgZI GCGATG 1 cut(s) 421
BtrI CACGTC 1 cut(s) 853
BtsIMutI CAGTG 6 cut(s) 14, 624, 806, 1803, 1809, 2226
Cac8I GCNNGC 8 cut(s) 58, 567, 1336, 1757, 1761, 1962, 2033, 2207
CciI TCATGA 3 cut(s) 1602, 1827, 1947
CfoI GCGC 3 cut(s) 895, 1121, 1165
Cfr10I RCCGGY 1 cut(s) 60
Cfr13I GGNCC 5 cut(s) 337, 379, 468, 792, 1268
CseI GACGC 3 cut(s) 110, 1405, 2136
Csp6I GTAC 2 cut(s) 346, 622
CviQI GTAC 2 cut(s) 346, 622
DdeI CTNAG 5 cut(s) 774, 1377, 1616, 1643, 1903
DpnI GATC 6 cut(s) 653, 695, 869, 1572, 1622, 1734
DpnII GATC 6 cut(s) 651, 693, 867, 1570, 1620, 1732
DriI GACNNNNNGTC 1 cut(s) 1611
EaeI YGGCCR 2 cut(s) 606, 655
EagI CGGCCG 1 cut(s) 655
Eam1104I CTCTTC 2 cut(s) 260, 1461
Eam1105I GACNNNNNGTC 1 cut(s) 1611
EarI CTCTTC 2 cut(s) 260, 1461
EciI GGCGGA 6 cut(s) 209, 329, 416, 587, 686, 1031
EclXI CGGCCG 1 cut(s) 655
Eco130I CCWWGG 2 cut(s) 153, 333
Eco24I GRGCYC 2 cut(s) 714, 2211
Eco31I GGTCTC 1 cut(s) 479
Eco47I GGWCC 3 cut(s) 337, 379, 792
Eco47III AGCGCT 1 cut(s) 1120
Eco52I CGGCCG 1 cut(s) 655
Eco57I CTGAAG 5 cut(s) 830, 944, 957, 1539, 2160
Eco81I CCTNAGG 1 cut(s) 774
Eco88I CYCGRG 1 cut(s) 902
EcoRI GAATTC 1 cut(s) 1801
EcoRII CCWGG 1 cut(s) 456
EcoT14I CCWWGG 2 cut(s) 153, 333
EcoT22I ATGCAT 2 cut(s) 1712, 1757
EcoT38I GRGCYC 2 cut(s) 714, 2211
ErhI CCWWGG 2 cut(s) 153, 333
Esp3I CGTCTC 4 cut(s) 44, 50, 1388, 1639
FaqI GGGAC 3 cut(s) 256, 1041, 2134
FauI CCCGC 7 cut(s) 294, 461, 509, 1253, 1258, 2024, 2198
FblI GTMKAC 2 cut(s) 636, 1286
Fnu4HI GCNGC 6 cut(s) 362, 405, 430, 570, 1065, 1506
FriOI GRGCYC 2 cut(s) 714, 2211
Fsp4HI GCNGC 6 cut(s) 362, 405, 430, 570, 1065, 1506
FspBI CTAG 3 cut(s) 858, 1736, 2254
GlaI GCGC 3 cut(s) 894, 1120, 1164
GluI GCNGC 6 cut(s) 362, 405, 430, 570, 1065, 1506
GsuI CTGGAG 1 cut(s) 152
HaeII RGCGCY 1 cut(s) 1122
HaeIII GGCC 6 cut(s) 447, 461, 470, 608, 657, 1270
HapII CCGG 6 cut(s) 61, 444, 466, 848, 889, 1730
HgaI GACGC 3 cut(s) 110, 1405, 2136
HhaI GCGC 3 cut(s) 895, 1121, 1165
Hin1I GRCGYC 2 cut(s) 102, 351
Hin6I GCGC 3 cut(s) 893, 1119, 1163
HinP1I GCGC 3 cut(s) 893, 1119, 1163
HindIII AAGCTT 1 cut(s) 2165
HinfI GANTC 9 cut(s) 158, 1109, 1213, 1280, 1297, 1469, 1484, 1846, 1951
HpaII CCGG 6 cut(s) 61, 444, 466, 848, 889, 1730
HphI GGTGA 7 cut(s) 286, 385, 442, 1043, 1051, 1850, 1855
Hpy166II GTNNAC 5 cut(s) 624, 637, 1174, 1287, 1519
Hpy188I TCNGA 5 cut(s) 1114, 1380, 1423, 1558, 1617
Hpy8I GTNNAC 5 cut(s) 624, 637, 1174, 1287, 1519
Hpy99I CGWCG 5 cut(s) 104, 332, 353, 356, 479
HpyCH4III ACNGT 6 cut(s) 9, 628, 958, 1419, 1701, 2221
HpyCH4IV ACGT 3 cut(s) 351, 558, 852
HpyF10VI GCNNNNNNNGC 5 cut(s) 453, 467, 919, 1171, 2144
HpyF3I CTNAG 5 cut(s) 774, 1377, 1616, 1643, 1903
HpySE526I ACGT 3 cut(s) 351, 558, 852
Hsp92I GRCGYC 2 cut(s) 102, 351
HspAI GCGC 3 cut(s) 893, 1119, 1163
Kzo9I GATC 6 cut(s) 651, 693, 867, 1570, 1620, 1732
LmnI GCTCC 1 cut(s) 177
Lsp1109I GCAGC 2 cut(s) 373, 1492
LweI GCATC 2 cut(s) 189, 1567
MaeI CTAG 3 cut(s) 858, 1736, 2254
MaeII ACGT 3 cut(s) 351, 558, 852
MaeIII GTNAC 8 cut(s) 721, 853, 1384, 1497, 1637, 2105, 2174, 2231
MalI GATC 6 cut(s) 653, 695, 869, 1572, 1622, 1734
MbiI CCGCTC 1 cut(s) 1260
MboI GATC 6 cut(s) 651, 693, 867, 1570, 1620, 1732
MfeI CAATTG 2 cut(s) 1671, 1965
MflI RGATCY 3 cut(s) 1570, 1620, 1732
MhlI GDGCHC 3 cut(s) 714, 1176, 2211
MluCI AATT 6 cut(s) 232, 1075, 1671, 1801, 1965, 2125
MlyI GAGTC 3 cut(s) 167, 1103, 1478
Mph1103I ATGCAT 2 cut(s) 1712, 1757
MroXI GAANNNNTTC 1 cut(s) 815
MseI TTAA 5 cut(s) 561, 1412, 1536, 1916, 2060
MslI CAYNNNNRTG 2 cut(s) 600, 1784
MspI CCGG 6 cut(s) 61, 444, 466, 848, 889, 1730
MspR9I CCNGG 3 cut(s) 458, 467, 890
MunI CAATTG 2 cut(s) 1671, 1965
MvaI CCWGG 1 cut(s) 458
MvnI CGCG 2 cut(s) 569, 2031
MwoI GCNNNNNNNGC 5 cut(s) 453, 467, 919, 1171, 2144
NciI CCSGG 2 cut(s) 467, 890
NcoI CCATGG 1 cut(s) 153
NdeII GATC 6 cut(s) 651, 693, 867, 1570, 1620, 1732
NlaIV GGNNCC 3 cut(s) 1269, 1791, 1997
NmeAIII GCCGAG 2 cut(s) 1040, 1191
NmuCI GTSAC 3 cut(s) 853, 2105, 2174
NsiI ATGCAT 2 cut(s) 1712, 1757
NspI RCATGY 1 cut(s) 1759
PaeI GCATGC 1 cut(s) 1759
PagI TCATGA 3 cut(s) 1602, 1827, 1947
PcsI WCGNNNNNNNCGW 3 cut(s) 480, 489, 660
PdmI GAANNNNTTC 1 cut(s) 815
PfeI GAWTC 6 cut(s) 1213, 1280, 1297, 1484, 1846, 1951
PflFI GACNNNGTC 1 cut(s) 326
PflMI CCANNNNNTGG 1 cut(s) 154
PkrI GCNGC 6 cut(s) 363, 406, 431, 571, 1066, 1507
PleI GAGTC 3 cut(s) 166, 1103, 1477
PpsI GAGTC 3 cut(s) 166, 1103, 1477
PshAI GACNNNNGTC 1 cut(s) 491
PsiI TTATAA 2 cut(s) 1074, 1182
Psp6I CCWGG 1 cut(s) 456
PspGI CCWGG 1 cut(s) 456
PspN4I GGNNCC 3 cut(s) 1269, 1791, 1997
PspPI GGNCC 5 cut(s) 337, 379, 468, 792, 1268
PstI CTGCAG 1 cut(s) 1883
PsuI RGATCY 3 cut(s) 1570, 1620, 1732
PsyI GACNNNGTC 1 cut(s) 326
RsaI GTAC 2 cut(s) 347, 623
RsaNI GTAC 2 cut(s) 346, 622
RseI CAYNNNNRTG 2 cut(s) 600, 1784
SaqAI TTAA 5 cut(s) 561, 1412, 1536, 1916, 2060
SatI GCNGC 6 cut(s) 362, 405, 430, 570, 1065, 1506
Sau3AI GATC 6 cut(s) 651, 693, 867, 1570, 1620, 1732
Sau96I GGNCC 5 cut(s) 337, 379, 468, 792, 1268
SchI GAGTC 3 cut(s) 167, 1103, 1478
ScrFI CCNGG 3 cut(s) 458, 467, 890
SduI GDGCHC 3 cut(s) 714, 1176, 2211
SfaNI GCATC 2 cut(s) 189, 1567
SfcI CTRYAG 3 cut(s) 1697, 1879, 2217
SfiI GGCCNNNNNGGCC 1 cut(s) 467
SinI GGWCC 3 cut(s) 337, 379, 792
SmiMI CAYNNNNRTG 2 cut(s) 600, 1784
SmlI CTYRAG 3 cut(s) 224, 682, 1007
SmoI CTYRAG 3 cut(s) 224, 682, 1007
SphI GCATGC 1 cut(s) 1759
Sse9I AATT 6 cut(s) 232, 1075, 1671, 1801, 1965, 2125
SspMI CTAG 3 cut(s) 858, 1736, 2254
StyD4I CCNGG 3 cut(s) 456, 465, 888
StyI CCWWGG 2 cut(s) 153, 333
TaaI ACNGT 6 cut(s) 9, 628, 958, 1419, 1701, 2221
TaiI ACGT 3 cut(s) 354, 561, 855
TasI AATT 6 cut(s) 232, 1075, 1671, 1801, 1965, 2125
TatI WGTACW 1 cut(s) 621
TauI GCSGC 4 cut(s) 407, 432, 572, 1067
TfiI GAWTC 6 cut(s) 1213, 1280, 1297, 1484, 1846, 1951
Tru1I TTAA 5 cut(s) 561, 1412, 1536, 1916, 2060
Tru9I TTAA 5 cut(s) 561, 1412, 1536, 1916, 2060
TscAI CASTG 6 cut(s) 14, 631, 813, 1803, 1816, 2226
TseFI GTSAC 3 cut(s) 853, 2105, 2174
TseI GCWGC 2 cut(s) 361, 1505
Tsp45I GTSAC 3 cut(s) 853, 2105, 2174
TspGWI ACGGA 2 cut(s) 32, 38
TspRI CASTG 6 cut(s) 14, 631, 813, 1803, 1816, 2226
Tth111I GACNNNGTC 1 cut(s) 326
Van91I CCANNNNNTGG 1 cut(s) 154
VneI GTGCAC 1 cut(s) 1172
VpaK11BI GGWCC 3 cut(s) 337, 379, 792
XapI RAATTY 1 cut(s) 1801
XceI RCATGY 1 cut(s) 1759
XcmI CCANNNNNNNNNTGG 1 cut(s) 1685
XmiI GTMKAC 2 cut(s) 636, 1286
XmnI GAANNNNTTC 1 cut(s) 815
XspI CTAG 3 cut(s) 858, 1736, 2254
ZraI GACGTC 1 cut(s) 352
Zsp2I ATGCAT 2 cut(s) 1712, 1757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.