FvH4_4g11993

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002052
Physical Location & Seq
Reverse (-)
15709458 .. 15711214
1757 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g11993.t1

Sequence Viewer

Length: 558 bp
ATGGGGTCTAAAGATTCTGTTGCCAACAATGTTTTAAAAAATGCGCCTTGGATGCGCCTTGAGGCTCAAAAGGCGCGAGGTGGTGCTGCAACTAAGTCAGCCTTGATGGTAAGATTTATCTCTTCTCCCAACTTTGGGTTTTATACTTGTTCGTTGTTTTTTGCTCTTGATCGATGGTCGTCTCTTCAAATTCATGCAAACTTTGACTGCTTTGACTCCTCTTTATATTTGTTGCGATTGATGGAGATAGGGGTATTGGAAAGTCTAGAAGAAGAAGAAGATGCACTAGATCATGAAGTGACAAATGTGACTGCAAGTGATTGTGGTAGTGCTCAAACTCCTCTTGTTGGTAGTTCTCAAACTCCTCTTGGTGGTAGTTCTATGGGTCAACCGAAGATGGTTTCCAAATACGGTCCGGGTTTTAAACCTCCTACAAGTCATGAGCTAAGGACTTGGATTTCGAAGGAAGAAGTTGAAGATGTTCAAAAGTTGGTGGTAGATCATAAAAAAGATTGGAGCAAGTATGGATGCACTATTATGTCGATGGTTGGACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

186

Amino Acids

20.39

Weight (kDa)

5.57

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 144 - 181 4.1e-07 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 76
AcsI RAATTY 1 cut(s) 189
AfiI CCNNNNNNNGG 4 cut(s) 134, 135, 347, 371
AgsI TTSAA 3 cut(s) 188, 476, 485
AluBI AGCT 1 cut(s) 445
AluI AGCT 1 cut(s) 445
Alw21I GWGCWC 1 cut(s) 334
Alw26I GTCTC 1 cut(s) 186
ApeKI GCWGC 1 cut(s) 86
ApoI RAATTY 1 cut(s) 189
ArsI GACNNNNNNTTYG 2 cut(s) 442, 474
Asp700I GAANNNNTTC 1 cut(s) 480
AspLEI GCGC 3 cut(s) 46, 57, 76
AspS9I GGNCC 1 cut(s) 413
AsuC2I CCSGG 1 cut(s) 417
AsuII TTCGAA 1 cut(s) 461
AvaII GGWCC 1 cut(s) 413
Bbv12I GWGCWC 1 cut(s) 334
BbvI GCAGC 1 cut(s) 73
BccI CCATC 5 cut(s) 100, 168, 235, 391, 538
BcnI CCSGG 1 cut(s) 417
BcoDI GTCTC 1 cut(s) 186
BfaI CTAG 2 cut(s) 266, 287
BisI GCNGC 1 cut(s) 87
BlsI GCNGC 1 cut(s) 88
Bme1390I CCNGG 1 cut(s) 417
Bme18I GGWCC 1 cut(s) 413
BmgT120I GGNCC 1 cut(s) 413
BmrFI CCNGG 1 cut(s) 417
BmsI GCATC 3 cut(s) 42, 271, 518
Bpu10I CCTNAGC 1 cut(s) 446
Bpu14I TTCGAA 1 cut(s) 461
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 1 cut(s) 417
Bsa29I ATCGAT 1 cut(s) 172
BsaJI CCNNGG 1 cut(s) 47
Bsc4I CCNNNNNNNGG 4 cut(s) 134, 135, 347, 371
BseCI ATCGAT 1 cut(s) 172
BseDI CCNNGG 1 cut(s) 47
BseGI GGATG 2 cut(s) 57, 533
BseLI CCNNNNNNNGG 4 cut(s) 134, 135, 347, 371
BseRI GAGGAG 3 cut(s) 208, 330, 354
BseXI GCAGC 1 cut(s) 73
Bsh1236I CGCG 1 cut(s) 76
BshVI ATCGAT 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 334
BsiSI CCGG 1 cut(s) 416
BslI CCNNNNNNNGG 4 cut(s) 134, 135, 347, 371
BsmAI GTCTC 1 cut(s) 186
BsmBI CGTCTC 1 cut(s) 186
Bsp119I TTCGAA 1 cut(s) 461
Bsp1286I GDGCHC 1 cut(s) 334
Bsp143I GATC 3 cut(s) 169, 289, 499
BspDI ATCGAT 1 cut(s) 172
BspFNI CGCG 1 cut(s) 76
BspHI TCATGA 2 cut(s) 292, 439
BspT104I TTCGAA 1 cut(s) 461
BssECI CCNNGG 1 cut(s) 47
BssMI GATC 3 cut(s) 169, 289, 499
BssT1I CCWWGG 1 cut(s) 47
Bst4CI ACNGT 2 cut(s) 413, 555
Bst6I CTCTTC 2 cut(s) 127, 189
BstBI TTCGAA 1 cut(s) 461
BstDEI CTNAG 2 cut(s) 93, 446
BstF5I GGATG 2 cut(s) 57, 533
BstFNI CGCG 1 cut(s) 76
BstHHI GCGC 3 cut(s) 46, 57, 76
BstKTI GATC 3 cut(s) 172, 292, 502
BstMAI GTCTC 1 cut(s) 186
BstMBI GATC 3 cut(s) 169, 289, 499
BstMWI GCNNNNNNNGC 2 cut(s) 52, 71
BstSCI CCNGG 1 cut(s) 415
BstUI CGCG 1 cut(s) 76
BstV1I GCAGC 1 cut(s) 73
Bsu15I ATCGAT 1 cut(s) 172
BsuTUI ATCGAT 1 cut(s) 172
BtsCI GGATG 2 cut(s) 57, 533
CciI TCATGA 2 cut(s) 292, 439
CfoI GCGC 3 cut(s) 46, 57, 76
Cfr13I GGNCC 1 cut(s) 413
ClaI ATCGAT 1 cut(s) 172
CpoI CGGWCCG 1 cut(s) 413
CspI CGGWCCG 1 cut(s) 413
CviAII CATG 3 cut(s) 194, 293, 440
CviJI RGCY 3 cut(s) 65, 101, 445
CviKI_1 RGCY 3 cut(s) 65, 101, 445
DdeI CTNAG 2 cut(s) 93, 446
DpnI GATC 3 cut(s) 171, 291, 501
DpnII GATC 3 cut(s) 169, 289, 499
DraI TTTAAA 2 cut(s) 36, 424
Eam1104I CTCTTC 2 cut(s) 127, 189
EarI CTCTTC 2 cut(s) 127, 189
Eco130I CCWWGG 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 413
EcoT14I CCWWGG 1 cut(s) 47
ErhI CCWWGG 1 cut(s) 47
Esp3I CGTCTC 1 cut(s) 186
FaeI CATG 3 cut(s) 197, 296, 443
FaiI YATR 9 cut(s) 144, 195, 226, 294, 383, 441, 504, 525, 539
FalI AAGNNNNNCTT 2 cut(s) 86, 118
FatI CATG 3 cut(s) 193, 292, 439
Fnu4HI GCNGC 1 cut(s) 87
FokI GGATG 2 cut(s) 64, 540
Fsp4HI GCNGC 1 cut(s) 87
FspBI CTAG 2 cut(s) 266, 287
GlaI GCGC 3 cut(s) 45, 56, 75
GluI GCNGC 1 cut(s) 87
HapII CCGG 1 cut(s) 416
HhaI GCGC 3 cut(s) 46, 57, 76
Hin1II CATG 3 cut(s) 197, 296, 443
Hin6I GCGC 3 cut(s) 44, 55, 74
HinP1I GCGC 3 cut(s) 44, 55, 74
HincII GTYRAC 1 cut(s) 389
HindII GTYRAC 1 cut(s) 389
HinfI GANTC 2 cut(s) 14, 215
HpaII CCGG 1 cut(s) 416
Hpy166II GTNNAC 1 cut(s) 389
Hpy188III TCNNGA 4 cut(s) 167, 266, 293, 440
Hpy8I GTNNAC 1 cut(s) 389
HpyAV CCTTC 1 cut(s) 457
HpyCH4III ACNGT 2 cut(s) 413, 555
HpyCH4V TGCA 5 cut(s) 89, 197, 284, 314, 531
HpyF10VI GCNNNNNNNGC 2 cut(s) 52, 71
HpyF3I CTNAG 2 cut(s) 93, 446
Hsp92II CATG 3 cut(s) 197, 296, 443
HspAI GCGC 3 cut(s) 44, 55, 74
Kzo9I GATC 3 cut(s) 169, 289, 499
LmnI GCTCC 1 cut(s) 516
LpnPI CCDG 1 cut(s) 429
Lsp1109I GCAGC 1 cut(s) 73
LweI GCATC 3 cut(s) 42, 271, 518
MaeI CTAG 2 cut(s) 266, 287
MaeIII GTNAC 2 cut(s) 298, 307
MalI GATC 3 cut(s) 171, 291, 501
MboI GATC 3 cut(s) 169, 289, 499
MboII GAAGA 9 cut(s) 114, 176, 281, 284, 287, 290, 406, 479, 488
MhlI GDGCHC 1 cut(s) 334
MluCI AATT 1 cut(s) 189
MlyI GAGTC 1 cut(s) 209
MmeI TCCRAC 1 cut(s) 529
MnlI CCTC 6 cut(s) 55, 71, 229, 351, 375, 438
MroXI GAANNNNTTC 1 cut(s) 480
MseI TTAA 2 cut(s) 35, 423
MslI CAYNNNNRTG 1 cut(s) 536
MspI CCGG 1 cut(s) 416
MspR9I CCNGG 1 cut(s) 417
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 2 cut(s) 52, 71
NciI CCSGG 1 cut(s) 417
NdeII GATC 3 cut(s) 169, 289, 499
NlaIII CATG 3 cut(s) 197, 296, 443
NmuCI GTSAC 2 cut(s) 298, 307
NspV TTCGAA 1 cut(s) 461
PagI TCATGA 2 cut(s) 292, 439
PdmI GAANNNNTTC 1 cut(s) 480
PfeI GAWTC 1 cut(s) 14
PkrI GCNGC 1 cut(s) 88
PleI GAGTC 1 cut(s) 209
PpsI GAGTC 1 cut(s) 209
PspPI GGNCC 1 cut(s) 413
RseI CAYNNNNRTG 1 cut(s) 536
Rsr2I CGGWCCG 1 cut(s) 413
RsrII CGGWCCG 1 cut(s) 413
SaqAI TTAA 2 cut(s) 35, 423
SatI GCNGC 1 cut(s) 87
Sau3AI GATC 3 cut(s) 169, 289, 499
Sau96I GGNCC 1 cut(s) 413
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 1 cut(s) 417
SduI GDGCHC 1 cut(s) 334
SetI ASST 3 cut(s) 82, 430, 447
SfaNI GCATC 3 cut(s) 42, 271, 518
SfuI TTCGAA 1 cut(s) 461
SinI GGWCC 1 cut(s) 413
SmiMI CAYNNNNRTG 1 cut(s) 536
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 1 cut(s) 189
SspMI CTAG 2 cut(s) 266, 287
StyD4I CCNGG 1 cut(s) 415
StyI CCWWGG 1 cut(s) 47
TaaI ACNGT 2 cut(s) 413, 555
TaqI TCGA 3 cut(s) 172, 461, 542
TasI AATT 1 cut(s) 189
TfiI GAWTC 1 cut(s) 14
Tru1I TTAA 2 cut(s) 35, 423
Tru9I TTAA 2 cut(s) 35, 423
TseFI GTSAC 2 cut(s) 298, 307
TseI GCWGC 1 cut(s) 86
Tsp45I GTSAC 2 cut(s) 298, 307
TspDTI ATGAA 2 cut(s) 182, 309
VpaK11BI GGWCC 1 cut(s) 413
XapI RAATTY 1 cut(s) 189
XbaI TCTAGA 1 cut(s) 265
XmnI GAANNNNTTC 1 cut(s) 480
XspI CTAG 2 cut(s) 266, 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.