RchiOBHm_Chr1g0361981

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002052
Physical Location & Seq
Forward (+)
53719805 .. 53721626
1822 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58683

Sequence Viewer

Length: 1329 bp
ATGAGGAAATTCACTGGCAACAAAGAGATTATTCGGCCGGCGGTTACAAGGTTTGCCACATCTTTTCTCTCTCTTCAAAGTTTATATAAGCAAAAGGAGGCTCTTATTAGTATGTTTTCATCAAAAGAATGGTATGAATGTGGATGTACAAAACATAAGGATGCTTATGCGGTGAGGAAGTGGATCCTTCGTGACACATATTTTTGGAACCATGTTGCTTATTGCATAAAGAGTGTCTTGCCCCTTGTTTGTGTCTTAAGAGAGGTTGATTCAGAAGTGAGACCCGCCATGGGATATATCTATGAGTTGATGGATGCCGCAAAAGATAAGATTGCAAATAATCTTGGAAATGTGGAACAAAAGTATGGACCGATTTGGAGGAGAATAGATAATAGGTGGAGTCCACAACTTCATCAACCATTGCATGCGGTGGGTTATTACTTGAACCCTCAATTCCGATATGACGACAACTTTAAAAATACCGAGCATGTGAAGAAGGGTTTGGAAGAGTGCATGGATAGGATGCTAGTTGGTAGGGCGCGTGTCACAGCGGAAATACAATTGGACTTATATGAGCGAAAGCTTGTGTTTTGGTGGGAAAAATATGGGACACAAACACCGGAGTTGATGAATTTTGCTACTCGGGTCCTTTCTCTTACTTGTAGAGCATCGGGGTGTGAGAGGAATTGGAGTACTTTTGAAATGATTCATACTAAAAAGAGGAATAGAGTTGAACACAAGAGGATGCATGCTTTAGTCTATGTGAAATATAATATTGCTCTAAAAGATAGGACTTTGAAAAGGAATGCTACAATGACCGATCCTATTGTAGTGGAAGAAATTGAATATGATGATGAATGGATAACAGAGATAGAGGATCCGGTTCTTCCCGCCGATCCACATTGGCTTGAGGACAATGTGGAAGATCTAACATTGAATGATGAGGCGGTAAGAAATGTGCCAATTGGTACATATCAAAGTACCCTAATTGATAGAGAGCCTCCTCCTCGTGTGCATTCTCCTCCTCACGAGCCTACTCCTCCCCGTGAGCCTACTCCTTCTCTTGATGAGCCTATTGTTTTATACAAAAGGAAATCTAGTGAAGGAGCATGTTCAAGTAAAAGAAAGGCTCCAAAGAAATCAATGCGTCTTGATGACATTGCGGATGATGGTATTAGAATTAATCCATATGATGAGACCAATCCTCTCTTCGAGCACCATGGTGATGATAGTGATGAAGGGGGTGATAGTGATGGTGGTGATAGTTTGGGTGATGATAGTTTGGATGGTGATCTTCTAATTGATGAGGATGATGATTTCCAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

442

Amino Acids

51.29

Weight (kDa)

5.38

Isoelectric Point (pI)

46.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimer_Tnp_hAT PF05699 193 - 259 6.4e-10 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 541
AciI CCGC 9 cut(s) 41, 170, 285, 318, 428, 551, 891, 947, 1163
AclWI GGATC 6 cut(s) 178, 191, 815, 872, 885, 890
AcoI YGGCCR 1 cut(s) 35
AcsI RAATTY 2 cut(s) 8, 631
AfaI GTAC 4 cut(s) 148, 694, 970, 982
AfiI CCNNNNNNNGG 1 cut(s) 290
AflII CTTAAG 1 cut(s) 256
AgsI TTSAA 8 cut(s) 77, 445, 701, 734, 799, 845, 937, 1116
AjuI GAANNNNNNNTTGG 4 cut(s) 485, 517, 1193, 1225
AleI CACNNNNGTG 1 cut(s) 1221
AluBI AGCT 1 cut(s) 583
AluI AGCT 1 cut(s) 583
Alw21I GWGCWC 1 cut(s) 1218
Alw26I GTCTC 2 cut(s) 274, 1190
AlwI GGATC 6 cut(s) 178, 191, 815, 872, 885, 890
Ama87I CYCGRG 1 cut(s) 642
AoxI GGCC 1 cut(s) 35
ApoI RAATTY 2 cut(s) 8, 631
AseI ATTAAT 1 cut(s) 1182
Asp700I GAANNNNTTC 1 cut(s) 705
AspLEI GCGC 1 cut(s) 541
AspS9I GGNCC 2 cut(s) 368, 646
AsuHPI GGTGA 6 cut(s) 184, 1235, 1256, 1271, 1283, 1301
AvaI CYCGRG 1 cut(s) 642
AvaII GGWCC 2 cut(s) 368, 646
BamHI GGATCC 2 cut(s) 183, 877
BauI CACGAG 2 cut(s) 1008, 1028
Bbv12I GWGCWC 1 cut(s) 1218
BccI CCATC 4 cut(s) 304, 1163, 1247, 1280
BcoDI GTCTC 2 cut(s) 274, 1190
BfaI CTAG 2 cut(s) 527, 1098
BfrI CTTAAG 1 cut(s) 256
BglII AGATCT 1 cut(s) 925
BisI GCNGC 1 cut(s) 318
BlsI GCNGC 1 cut(s) 319
BmcAI AGTACT 1 cut(s) 694
Bme18I GGWCC 2 cut(s) 368, 646
BmeT110I CYCGRG 1 cut(s) 642
BmgT120I GGNCC 2 cut(s) 368, 646
BmiI GGNNCC 5 cut(s) 185, 209, 647, 879, 1131
BmsI GCATC 5 cut(s) 151, 304, 513, 677, 735
BpuEI CTTGAG 1 cut(s) 929
BsaBI GATNNNNATC 1 cut(s) 1290
BsaI GGTCTC 2 cut(s) 274, 1190
BsaJI CCNNGG 3 cut(s) 288, 1219, 1321
BsaWI WCCGGW 2 cut(s) 619, 880
Bsc4I CCNNNNNNNGG 1 cut(s) 290
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 1 cut(s) 19
Bse3DI GCAATG 2 cut(s) 419, 1158
Bse8I GATNNNNATC 1 cut(s) 1290
BseDI CCNNGG 3 cut(s) 288, 1219, 1321
BseGI GGATG 8 cut(s) 149, 166, 319, 528, 750, 1171, 1291, 1315
BseJI GATNNNNATC 1 cut(s) 1290
BseLI CCNNNNNNNGG 1 cut(s) 290
BseMI GCAATG 2 cut(s) 419, 1158
BseNI ACTGG 1 cut(s) 19
BseRI GAGGAG 6 cut(s) 394, 993, 996, 1011, 1014, 1029
BseX3I CGGCCG 1 cut(s) 35
Bsh1236I CGCG 1 cut(s) 541
Bsh1285I CGRYCG 1 cut(s) 38
BshFI GGCC 1 cut(s) 37
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 1 cut(s) 1218
BsiHKCI CYCGRG 1 cut(s) 642
BsiSI CCGG 3 cut(s) 38, 620, 881
BslFI GGGAC 1 cut(s) 622
BslI CCNNNNNNNGG 1 cut(s) 290
BsmAI GTCTC 2 cut(s) 274, 1190
BsmFI GGGAC 1 cut(s) 622
BsmI GAATGC 2 cut(s) 811, 1015
BsnI GGCC 1 cut(s) 37
Bso31I GGTCTC 2 cut(s) 274, 1190
BsoBI CYCGRG 1 cut(s) 642
Bsp1286I GDGCHC 1 cut(s) 1218
Bsp1407I TGTACA 1 cut(s) 146
Bsp143I GATC 6 cut(s) 183, 820, 877, 895, 925, 1291
Bsp19I CCATGG 2 cut(s) 288, 1219
BspACI CCGC 9 cut(s) 41, 170, 285, 318, 428, 551, 891, 947, 1163
BspANI GGCC 1 cut(s) 37
BspFNI CGCG 1 cut(s) 541
BspLI GGNNCC 5 cut(s) 185, 209, 647, 879, 1131
BspPI GGATC 6 cut(s) 178, 191, 815, 872, 885, 890
BspTI CTTAAG 1 cut(s) 256
BspTNI GGTCTC 2 cut(s) 274, 1190
BsrDI GCAATG 2 cut(s) 419, 1158
BsrFI RCCGGY 1 cut(s) 37
BsrGI TGTACA 1 cut(s) 146
BsrI ACTGG 1 cut(s) 19
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 3 cut(s) 288, 1219, 1321
BssMI GATC 6 cut(s) 183, 820, 877, 895, 925, 1291
BssSI CACGAG 2 cut(s) 1008, 1028
BssT1I CCWWGG 3 cut(s) 288, 1219, 1321
Bst2BI CACGAG 2 cut(s) 1008, 1028
Bst6I CTCTTC 3 cut(s) 78, 501, 1214
BstAFI CTTAAG 1 cut(s) 256
BstAUI TGTACA 1 cut(s) 146
BstC8I GCNNGC 3 cut(s) 39, 426, 750
BstDSI CCRYGG 2 cut(s) 288, 1219
BstF5I GGATG 8 cut(s) 149, 166, 319, 528, 750, 1171, 1291, 1315
BstFNI CGCG 1 cut(s) 541
BstHHI GCGC 1 cut(s) 541
BstKTI GATC 6 cut(s) 186, 823, 880, 898, 928, 1294
BstMAI GTCTC 2 cut(s) 274, 1190
BstMBI GATC 6 cut(s) 183, 820, 877, 895, 925, 1291
BstMCI CGRYCG 1 cut(s) 38
BstNSI RCATGY 4 cut(s) 428, 491, 752, 1113
BstUI CGCG 1 cut(s) 541
BstX2I RGATCY 3 cut(s) 183, 877, 925
BstYI RGATCY 3 cut(s) 183, 877, 925
BstZI CGGCCG 1 cut(s) 35
BsuRI GGCC 1 cut(s) 37
BtgI CCRYGG 2 cut(s) 288, 1219
BtsCI GGATG 8 cut(s) 149, 166, 319, 528, 750, 1171, 1291, 1315
BtsIMutI CAGTG 1 cut(s) 12
Cac8I GCNNGC 3 cut(s) 39, 426, 750
CfoI GCGC 1 cut(s) 541
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 2 cut(s) 368, 646
CseI GACGC 1 cut(s) 1136
Csp6I GTAC 4 cut(s) 147, 693, 969, 981
CviAII CATG 8 cut(s) 212, 289, 425, 488, 514, 749, 1110, 1220
CviJI RGCY 9 cut(s) 37, 101, 583, 907, 1000, 1033, 1051, 1072, 1130
CviKI_1 RGCY 9 cut(s) 37, 101, 583, 907, 1000, 1033, 1051, 1072, 1130
CviQI GTAC 4 cut(s) 147, 693, 969, 981
DpnI GATC 6 cut(s) 185, 822, 879, 897, 927, 1293
DpnII GATC 6 cut(s) 183, 820, 877, 895, 925, 1291
DraI TTTAAA 1 cut(s) 475
EaeI YGGCCR 1 cut(s) 35
EagI CGGCCG 1 cut(s) 35
Eam1104I CTCTTC 3 cut(s) 78, 501, 1214
EarI CTCTTC 3 cut(s) 78, 501, 1214
EclXI CGGCCG 1 cut(s) 35
Eco130I CCWWGG 3 cut(s) 288, 1219, 1321
Eco31I GGTCTC 2 cut(s) 274, 1190
Eco47I GGWCC 2 cut(s) 368, 646
Eco52I CGGCCG 1 cut(s) 35
Eco88I CYCGRG 1 cut(s) 642
EcoO109I RGGNCCY 1 cut(s) 646
EcoT14I CCWWGG 3 cut(s) 288, 1219, 1321
EcoT22I ATGCAT 1 cut(s) 750
ErhI CCWWGG 3 cut(s) 288, 1219, 1321
FaeI CATG 8 cut(s) 215, 292, 428, 491, 517, 752, 1113, 1223
FalI AAGNNNNNCTT 2 cut(s) 221, 253
FaqI GGGAC 1 cut(s) 622
FatI CATG 8 cut(s) 211, 288, 424, 487, 513, 748, 1109, 1219
FauI CCCGC 2 cut(s) 292, 898
FauNDI CATATG 1 cut(s) 1189
Fnu4HI GCNGC 1 cut(s) 318
FokI GGATG 8 cut(s) 156, 173, 326, 535, 757, 1178, 1298, 1322
Fsp4HI GCNGC 1 cut(s) 318
FspBI CTAG 2 cut(s) 527, 1098
GlaI GCGC 1 cut(s) 540
GluI GCNGC 1 cut(s) 318
HaeIII GGCC 1 cut(s) 37
HapII CCGG 3 cut(s) 38, 620, 881
HgaI GACGC 1 cut(s) 1136
HhaI GCGC 1 cut(s) 541
Hin1II CATG 8 cut(s) 215, 292, 428, 491, 517, 752, 1113, 1223
Hin6I GCGC 1 cut(s) 539
HinP1I GCGC 1 cut(s) 539
HindIII AAGCTT 1 cut(s) 581
HinfI GANTC 3 cut(s) 269, 400, 706
HpaII CCGG 3 cut(s) 38, 620, 881
HphI GGTGA 6 cut(s) 184, 1235, 1256, 1271, 1283, 1301
Hpy166II GTNNAC 1 cut(s) 404
Hpy188I TCNGA 2 cut(s) 274, 458
Hpy188III TCNNGA 4 cut(s) 191, 1028, 1064, 1151
Hpy8I GTNNAC 1 cut(s) 404
HpyAV CCTTC 5 cut(s) 197, 490, 1068, 1097, 1232
HpyCH4V TGCA 6 cut(s) 225, 335, 424, 513, 748, 1015
Hsp92II CATG 8 cut(s) 215, 292, 428, 491, 517, 752, 1113, 1223
HspAI GCGC 1 cut(s) 539
KroI GCCGGC 1 cut(s) 37
KroNI GCCGGC 1 cut(s) 39
Kzo9I GATC 6 cut(s) 183, 820, 877, 895, 925, 1291
LmnI GCTCC 2 cut(s) 1106, 1135
LpnPI CCDG 3 cut(s) 51, 633, 894
LweI GCATC 5 cut(s) 151, 304, 513, 677, 735
MaeI CTAG 2 cut(s) 527, 1098
MaeIII GTNAC 3 cut(s) 43, 191, 544
MalI GATC 6 cut(s) 185, 822, 879, 897, 927, 1293
MboI GATC 6 cut(s) 183, 820, 877, 895, 925, 1291
MboII GAAGA 8 cut(s) 65, 505, 518, 848, 878, 935, 1201, 1286
MfeI CAATTG 2 cut(s) 560, 963
MflI RGATCY 3 cut(s) 183, 877, 925
MhlI GDGCHC 1 cut(s) 1218
MlyI GAGTC 1 cut(s) 409
Mph1103I ATGCAT 1 cut(s) 750
MroNI GCCGGC 1 cut(s) 37
MroXI GAANNNNTTC 1 cut(s) 705
MseI TTAA 3 cut(s) 257, 474, 1182
MslI CAYNNNNRTG 4 cut(s) 159, 673, 1221, 1224
MspA1I CMGCKG 1 cut(s) 551
MspCI CTTAAG 1 cut(s) 256
MspI CCGG 3 cut(s) 38, 620, 881
MunI CAATTG 2 cut(s) 560, 963
Mva1269I GAATGC 2 cut(s) 811, 1015
MvnI CGCG 1 cut(s) 541
NaeI GCCGGC 1 cut(s) 39
NcoI CCATGG 2 cut(s) 288, 1219
NdeI CATATG 1 cut(s) 1189
NdeII GATC 6 cut(s) 183, 820, 877, 895, 925, 1291
NgoMIV GCCGGC 1 cut(s) 37
NlaIII CATG 8 cut(s) 215, 292, 428, 491, 517, 752, 1113, 1223
NlaIV GGNNCC 5 cut(s) 185, 209, 647, 879, 1131
NmuCI GTSAC 2 cut(s) 191, 544
NsiI ATGCAT 1 cut(s) 750
NspI RCATGY 4 cut(s) 428, 491, 752, 1113
OliI CACNNNNGTG 1 cut(s) 1221
PaeI GCATGC 2 cut(s) 428, 752
PctI GAATGC 2 cut(s) 811, 1015
PdiI GCCGGC 1 cut(s) 39
PdmI GAANNNNTTC 1 cut(s) 705
PfeI GAWTC 2 cut(s) 269, 706
PkrI GCNGC 1 cut(s) 319
PleI GAGTC 1 cut(s) 408
PpsI GAGTC 1 cut(s) 408
PpuMI RGGWCCY 1 cut(s) 646
PshBI ATTAAT 1 cut(s) 1182
Psp5II RGGWCCY 1 cut(s) 646
PspN4I GGNNCC 5 cut(s) 185, 209, 647, 879, 1131
PspPI GGNCC 2 cut(s) 368, 646
PspPPI RGGWCCY 1 cut(s) 646
PsuI RGATCY 3 cut(s) 183, 877, 925
RsaI GTAC 4 cut(s) 148, 694, 970, 982
RsaNI GTAC 4 cut(s) 147, 693, 969, 981
RseI CAYNNNNRTG 4 cut(s) 159, 673, 1221, 1224
SaqAI TTAA 3 cut(s) 257, 474, 1182
SatI GCNGC 1 cut(s) 318
Sau3AI GATC 6 cut(s) 183, 820, 877, 895, 925, 1291
Sau96I GGNCC 2 cut(s) 368, 646
ScaI AGTACT 1 cut(s) 694
SchI GAGTC 1 cut(s) 409
SduI GDGCHC 1 cut(s) 1218
SetI ASST 4 cut(s) 53, 267, 398, 585
SfaNI GCATC 5 cut(s) 151, 304, 513, 677, 735
SinI GGWCC 2 cut(s) 368, 646
SmiMI CAYNNNNRTG 4 cut(s) 159, 673, 1221, 1224
SmlI CTYRAG 2 cut(s) 256, 908
SmoI CTYRAG 2 cut(s) 256, 908
SphI GCATGC 2 cut(s) 428, 752
SsiI CCGC 9 cut(s) 41, 170, 285, 318, 428, 551, 891, 947, 1163
SspI AATATT 1 cut(s) 775
SspMI CTAG 2 cut(s) 527, 1098
StyI CCWWGG 3 cut(s) 288, 1219, 1321
TaqI TCGA 1 cut(s) 1212
TaqII GACCGA 2 cut(s) 385, 833
TatI WGTACW 2 cut(s) 146, 692
TauI GCSGC 1 cut(s) 320
TfiI GAWTC 2 cut(s) 269, 706
Tru1I TTAA 3 cut(s) 257, 474, 1182
Tru9I TTAA 3 cut(s) 257, 474, 1182
TscAI CASTG 1 cut(s) 19
TseFI GTSAC 2 cut(s) 191, 544
Tsp45I GTSAC 2 cut(s) 191, 544
TspDTI ATGAA 7 cut(s) 108, 150, 401, 644, 698, 870, 1251
TspRI CASTG 1 cut(s) 19
Vha464I CTTAAG 1 cut(s) 256
VpaK11BI GGWCC 2 cut(s) 368, 646
VspI ATTAAT 1 cut(s) 1182
XapI RAATTY 2 cut(s) 8, 631
XceI RCATGY 4 cut(s) 428, 491, 752, 1113
XmnI GAANNNNTTC 1 cut(s) 705
XspI CTAG 2 cut(s) 527, 1098
ZrmI AGTACT 1 cut(s) 694
Zsp2I ATGCAT 1 cut(s) 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.