Rroxscaffold_4G00304630

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002052
Physical Location & Seq
Forward (+)
25274101 .. 25276932
2832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00304630.1

Sequence Viewer

Length: 891 bp
ATGAGTCAACCGAAGTCAAGAGGACTGATGGACAAGTTTGTGTCCTCAAAAGCCTGGCAAGCTACATTGAATTCTTCATTCATGAAAGAAGAAAGACGTAATGTGTGTAGGGCGATTGGGCGGTTGTTCTATACTAGTGCCATAGCATTCAATGTGGCAAATAATCCTTATTATTTTGCGGCATTGGCGCAAGTTTCAAAGTACGACCCTGGTTTTCAACCTCCAACTAGTCATGAGCTAAGGACTTGGATTTTGAAGAAAGAAGTTGTAGATGTTCAAAAAATGATGTTAGAGCACAAAAAAGATTGGAGCCATTATGGATGCACAATTATGTCGGATGGTTGGATTGATGGCAAAAGTAGGATTATACTAAATTTTTTAGTAAATAGCCCTAGAGGTACATGGTTCTTGAAATCGGTTGATGCATTGGATACAATTAAAAATGGGGAGTTGATGCTCAATTATTTGGATAGTGTTGTGGAGGAGGTAGGGGAGGAGAATGTGGTCCAAATAGTTACTGACAATGCTTCTAATTATAAGTGGGTGGGAAAGGAACTTATGAAGCATAGAAGCAAATTGTGGTGGACTCCATGTGCGGCTTATTGCATTGACTTGATGTTGGAGGATATAAGCAAATTGAAAGTCTTTGAAACTACCATTCAAAGAGCTAAGCAAATTGTGAAGTTTATTTATGGGCACACACAAGTTCTTTCCATTATGAGAAAATTCACCAAGAACAAGGAGATTATTCGTCCGGCGGTTACAAGGTTGCCACATCTTTTCTCTCTCTTCAAAGTTTATATAAGCAAAAGGAAGCTCTCATTAGTATGTTTTCATCTAAAGATTGGAATGAATGTGGATGCACAAAATATAAAGATGCTTATGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

296

Amino Acids

34.34

Weight (kDa)

9.59

Isoelectric Point (pI)

29.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 75 - 226 1.3e-60 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 537
AciI CCGC 4 cut(s) 121, 179, 596, 758
AcsI RAATTY 3 cut(s) 70, 373, 725
AfaI GTAC 2 cut(s) 203, 400
AhlI ACTAGT 2 cut(s) 134, 227
AjnI CCWGG 2 cut(s) 53, 208
AluBI AGCT 4 cut(s) 62, 238, 668, 817
AluI AGCT 4 cut(s) 62, 238, 668, 817
Alw21I GWGCWC 1 cut(s) 297
ApoI RAATTY 3 cut(s) 70, 373, 725
ArsI GACNNNNNNTTYG 4 cut(s) 235, 267, 627, 659
AspLEI GCGC 1 cut(s) 190
AspS9I GGNCC 1 cut(s) 505
AsuHPI GGTGA 1 cut(s) 721
AvaII GGWCC 1 cut(s) 505
BaeGI GKGCMC 1 cut(s) 699
Bbv12I GWGCWC 1 cut(s) 297
BccI CCATC 3 cut(s) 22, 332, 344
BciT130I CCWGG 2 cut(s) 55, 210
BciVI GTATCC 1 cut(s) 424
BcuI ACTAGT 2 cut(s) 134, 227
BfaI CTAG 3 cut(s) 135, 228, 393
BfuI GTATCC 1 cut(s) 424
BisI GCNGC 2 cut(s) 180, 597
BlpI GCTNAGC 1 cut(s) 669
BlsI GCNGC 2 cut(s) 181, 598
Bme1390I CCNGG 2 cut(s) 55, 210
Bme18I GGWCC 1 cut(s) 505
BmgT120I GGNCC 1 cut(s) 505
BmiI GGNNCC 1 cut(s) 311
BmrFI CCNGG 2 cut(s) 55, 210
BmsI GCATC 5 cut(s) 311, 412, 444, 850, 867
Bpu10I CCTNAGC 1 cut(s) 239
Bpu1102I GCTNAGC 1 cut(s) 669
BsaJI CCNNGG 1 cut(s) 208
BseBI CCWGG 2 cut(s) 55, 210
BseDI CCNNGG 1 cut(s) 208
BseGI GGATG 3 cut(s) 326, 343, 865
BseRI GAGGAG 2 cut(s) 497, 509
BseSI GKGCMC 1 cut(s) 699
BsiHKAI GWGCWC 1 cut(s) 297
BsiSI CCGG 1 cut(s) 755
BsmI GAATGC 1 cut(s) 146
Bsp1286I GDGCHC 2 cut(s) 297, 699
Bsp1720I GCTNAGC 1 cut(s) 669
BspACI CCGC 4 cut(s) 121, 179, 596, 758
BspHI TCATGA 2 cut(s) 81, 232
BspLI GGNNCC 1 cut(s) 311
BssECI CCNNGG 1 cut(s) 208
Bst2UI CCWGG 2 cut(s) 55, 210
Bst6I CTCTTC 1 cut(s) 794
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 2 cut(s) 239, 669
BstF5I GGATG 3 cut(s) 326, 343, 865
BstHHI GCGC 1 cut(s) 190
BstMWI GCNNNNNNNGC 2 cut(s) 59, 185
BstNI CCWGG 2 cut(s) 55, 210
BstSCI CCNGG 2 cut(s) 53, 208
BstSLI GKGCMC 1 cut(s) 699
BsuI GTATCC 1 cut(s) 424
BtsCI GGATG 3 cut(s) 326, 343, 865
Cac8I GCNNGC 1 cut(s) 60
CciI TCATGA 2 cut(s) 81, 232
CfoI GCGC 1 cut(s) 190
Cfr13I GGNCC 1 cut(s) 505
Csp6I GTAC 2 cut(s) 202, 399
CviAII CATG 4 cut(s) 82, 233, 402, 591
CviJI RGCY 8 cut(s) 53, 62, 238, 312, 390, 599, 668, 817
CviKI_1 RGCY 8 cut(s) 53, 62, 238, 312, 390, 599, 668, 817
CviQI GTAC 2 cut(s) 202, 399
DdeI CTNAG 2 cut(s) 239, 669
Eam1104I CTCTTC 1 cut(s) 794
EarI CTCTTC 1 cut(s) 794
Eco47I GGWCC 1 cut(s) 505
EcoRI GAATTC 1 cut(s) 70
EcoRII CCWGG 2 cut(s) 53, 208
EcoT22I ATGCAT 1 cut(s) 427
FaeI CATG 4 cut(s) 85, 236, 405, 594
FatI CATG 4 cut(s) 81, 232, 401, 590
Fnu4HI GCNGC 2 cut(s) 180, 597
FokI GGATG 3 cut(s) 333, 350, 872
Fsp4HI GCNGC 2 cut(s) 180, 597
FspBI CTAG 3 cut(s) 135, 228, 393
GlaI GCGC 1 cut(s) 189
GluI GCNGC 2 cut(s) 180, 597
HapII CCGG 1 cut(s) 755
HhaI GCGC 1 cut(s) 190
Hin1II CATG 4 cut(s) 85, 236, 405, 594
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HincII GTYRAC 1 cut(s) 8
HindII GTYRAC 1 cut(s) 8
HinfI GANTC 2 cut(s) 4, 586
HpaII CCGG 1 cut(s) 755
HphI GGTGA 1 cut(s) 721
Hpy166II GTNNAC 2 cut(s) 8, 585
Hpy188I TCNGA 1 cut(s) 337
Hpy188III TCNNGA 4 cut(s) 18, 82, 233, 409
Hpy8I GTNNAC 2 cut(s) 8, 585
HpyCH4IV ACGT 1 cut(s) 97
HpyCH4V TGCA 4 cut(s) 324, 425, 606, 863
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 185
HpyF3I CTNAG 2 cut(s) 239, 669
HpySE526I ACGT 1 cut(s) 97
Hsp92II CATG 4 cut(s) 85, 236, 405, 594
HspAI GCGC 1 cut(s) 188
LmnI GCTCC 1 cut(s) 309
LpnPI CCDG 5 cut(s) 40, 67, 195, 222, 768
LweI GCATC 5 cut(s) 311, 412, 444, 850, 867
MaeI CTAG 3 cut(s) 135, 228, 393
MaeII ACGT 1 cut(s) 97
MaeIII GTNAC 2 cut(s) 514, 760
MboII GAAGA 4 cut(s) 66, 101, 268, 781
MhlI GDGCHC 2 cut(s) 297, 699
MlyI GAGTC 2 cut(s) 13, 580
MmeI TCCRAC 4 cut(s) 248, 315, 323, 600
MnlI CCTC 8 cut(s) 14, 55, 231, 389, 475, 478, 487, 616
Mph1103I ATGCAT 1 cut(s) 427
MseI TTAA 1 cut(s) 438
MslI CAYNNNNRTG 2 cut(s) 329, 826
MspI CCGG 1 cut(s) 755
MspR9I CCNGG 2 cut(s) 55, 210
Mva1269I GAATGC 1 cut(s) 146
MvaI CCWGG 2 cut(s) 55, 210
MwoI GCNNNNNNNGC 2 cut(s) 59, 185
NlaIII CATG 4 cut(s) 85, 236, 405, 594
NlaIV GGNNCC 1 cut(s) 311
NsiI ATGCAT 1 cut(s) 427
PagI TCATGA 2 cut(s) 81, 232
PctI GAATGC 1 cut(s) 146
PkrI GCNGC 2 cut(s) 181, 598
PleI GAGTC 2 cut(s) 12, 580
PpsI GAGTC 2 cut(s) 12, 580
PsiI TTATAA 1 cut(s) 537
Psp6I CCWGG 2 cut(s) 53, 208
PspGI CCWGG 2 cut(s) 53, 208
PspN4I GGNNCC 1 cut(s) 311
PspPI GGNCC 1 cut(s) 505
RsaI GTAC 2 cut(s) 203, 400
RsaNI GTAC 2 cut(s) 202, 399
RseI CAYNNNNRTG 2 cut(s) 329, 826
SaqAI TTAA 1 cut(s) 438
SatI GCNGC 2 cut(s) 180, 597
Sau96I GGNCC 1 cut(s) 505
SchI GAGTC 2 cut(s) 13, 580
ScrFI CCNGG 2 cut(s) 55, 210
SduI GDGCHC 2 cut(s) 297, 699
SetI ASST 9 cut(s) 64, 100, 223, 240, 400, 489, 670, 770, 819
SfaNI GCATC 5 cut(s) 311, 412, 444, 850, 867
SinI GGWCC 1 cut(s) 505
SmiMI CAYNNNNRTG 2 cut(s) 329, 826
SpeI ACTAGT 2 cut(s) 134, 227
SsiI CCGC 4 cut(s) 121, 179, 596, 758
SspMI CTAG 3 cut(s) 135, 228, 393
StyD4I CCNGG 2 cut(s) 53, 208
TaiI ACGT 1 cut(s) 100
TauI GCSGC 2 cut(s) 182, 599
Tru1I TTAA 1 cut(s) 438
Tru9I TTAA 1 cut(s) 438
TspDTI ATGAA 6 cut(s) 66, 70, 98, 575, 824, 866
VpaK11BI GGWCC 1 cut(s) 505
XapI RAATTY 3 cut(s) 70, 373, 725
XspI CTAG 3 cut(s) 135, 228, 393
Zsp2I ATGCAT 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.