Rw4G019360

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002052
Physical Location & Seq
Reverse (-)
42608475 .. 42611214
2740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G019360.1

Sequence Viewer

Length: 2274 bp
ATGCAGGTAACAATGAGTTCTAGTACTACTACTAGTGGGACAACAAAGAAAGATCATGCTTGGAAATGGCCTAAACCAATTCCGGGGGAGTTGAAGTACTTTTTGTGTGCGTTTTGTGATAAAAAAAATACCGGTGGCATTTTCATATTCAAGCAGCATTTAGTTGGCACCCATAAAGGAATCAAAGCTTGTCGAAAAGTCCCACTAAATGTGAAGGATTGGTGCACTTTAGCACTTAAAAGAAATGAAGAAGAGAAACAAGCAAGGATTACGGTGCGTAGGGAGATAGGGGGATTGGAGCCACTAGAAGAGGAAGATGCACCTGTTCAAGCAATGGAAAATGTGGCTGCAAGTGAGATTGGTAGTGCTCAAACTCCACTTGGTGGTGGTTCTACGAGTCAACCGAAGCCAAGAGGACCGATGGACAAGTTTGTGTCCTCAAAAGCTCGGCAAGCTACATTGAATTCTTCATTCAAGAAAGAAGAAAGACGTAATGTTGCCATAGCATTCAATGTGGCAAATAATCCTTATTATTTTGCGGCATTGGAGCAAGTTTCCAAGGACGGCCCCGGTTTTCAACCTCCAACTAGTCATGAGCTAAGGACTTGGATTTTGAAGGAAGAAGTTGTAGATGTTCAAAAAATGATGTTAGAGCACAAAAAAAATTTGAGCCATTATGGATGCACAATTATGTCGGATGGTTGGACGGATGGCAAAAGTAGGGTTATACTAAATTTTTTAGTAAATAGCCCTAGAGGTACATGGTTTTTGAAATCAGTTGATGCATCAGATACAATTAAAAATGGGGAGTTGATGCTCAATTATTTGGATAGTGTTGTGGAGGAGGTAGGGGAGGAGAATGTGGTCCAAATAGTTACCGACAATACTTCTAATTATAAGTGGGTGGGAAAGGAACTTATGAAGCATAGAAGCAAATTGTGGTGGACTCCATGTGCGGCTCATTGCATTGACTTGATGTTGGAGGATATAAGCAAGTTGAAAGTCTTTGAAACTACCATTCAAAGAGCTAAGCAAATTGTGAAGTTTATTTATGGGCACACACAAGTTCTTTCCATTATGAGAAAATTCACCAAGAACAAGGAGATTATTCGTCCGGCGGTTACAAGGTTTGCCACATCTTTTCTCTCTCTTCAAAGTTTATATAAGAAAAAGGAAGCTCTCATTAGTATGTTTTCATCTAAAGATTGGAATGAATGTGGATGCACAAAACATAAAGATGCAAATGATGTGAGGAAGTGGATACTTCATGATGGAAATTTTTGGAACCATGTTGCTTATTGCATAAAAAGTGTTCTACCACTTGTTTGTGTTTTGAGAGAGGTTGATTCGGAAGTGAGACCGCTATGGGATTTATCTATGACTAAGAATCTTGGAAATGTGGAAGCAAAGTATGGTCCAATTTGGAGGAGAATAGATAATAGATGGAGTCCACAACTTCATCAACCATTGCATGCAGCGGGTTATTACTTGAACCCTCAATTTCGGTATGAAGAAAACTTTTCAAATACCGAGCATGTGAAGCAGGGTTTGGAAGAGTGCATGGATAGGATGCTAGTCGGTGAGGATCGTATTAAAGCGGAGATACAATTAGACTTATATGAGCGAAAGCTCGGTAAGTTTGGAAGTGAAATGGCCTTGTCTAATAGGAAGAAGCGATCACTCGAAAAATATGGGACACAGACACCGGAGTTGATGACTTTTGCTATTTGGATTCATACCAAAAAGAGGAATAGGCTTGAGCATAAGAGGTTGCATGCTTTGGTCTATGTGAAATACAATGTTGCTCTAAGAGATAGGACTAAGAAAAGAAATGCCACAATGATCGATCCTATTGTTGTGGAAGAAATTGAATCCGATGATGAATGGATAACGGAGATAGAGGATCCGGTTCTTCCTACTGATCCTAATTGGCTTGAGGATAGAATAGAAGATCTAACATTGAATGATGAGGCGGTAAGGAATGTGCCAATTGGTACATACCAAAGTACCCTAATAGATAGAGAGCCTACTCCTCGTGAGGCTTCTCCTCCTCCCCGTGAGCCTACTCCTTCTCTTGATGAGCCTATTGTTTTATACAAAAGGAAATCTAGTCAAGCACCAAGTTCAAGTAGAAGAAAGCTTCCAAGGAAATCAATGCGTCTTGAAGACATTGCGGATGATGATATTAGAACTAATCCATATGAAGATACTAATCCTCTCTTTGATTTGGATGGTGATGTTCTAGTTGATAAGGATGATGATTTCCAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

757

Amino Acids

87.04

Weight (kDa)

8.35

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 195 - 346 2.5e-60 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 897
AccB1I GGYRCC 1 cut(s) 167
AccB7I CCANNNNNTGG 1 cut(s) 383
AciI CCGC 8 cut(s) 539, 956, 1118, 1361, 1478, 1598, 1973, 2174
AclWI GGATC 5 cut(s) 1593, 1841, 1898, 1911, 1916
AcsI RAATTY 5 cut(s) 463, 664, 733, 1085, 1276
AdeI CACNNNGTG 1 cut(s) 383
AfaI GTAC 5 cut(s) 25, 98, 760, 1996, 2008
AfiI CCNNNNNNNGG 3 cut(s) 83, 383, 1746
AgeI ACCGGT 1 cut(s) 131
AhlI ACTAGT 2 cut(s) 32, 587
AjuI GAANNNNNNNTTGG 2 cut(s) 1532, 1564
AluBI AGCT 8 cut(s) 188, 446, 455, 598, 1028, 1178, 1630, 2140
AluI AGCT 8 cut(s) 188, 446, 455, 598, 1028, 1178, 1630, 2140
Alw21I GWGCWC 3 cut(s) 227, 370, 657
Alw26I GTCTC 1 cut(s) 1351
Alw44I GTGCAC 1 cut(s) 223
AlwI GGATC 5 cut(s) 1593, 1841, 1898, 1911, 1916
AoxI GGCC 3 cut(s) 68, 565, 1653
ApaLI GTGCAC 1 cut(s) 223
ApeKI GCWGC 3 cut(s) 154, 347, 1475
ApoI RAATTY 5 cut(s) 463, 664, 733, 1085, 1276
ArsI GACNNNNNNTTYG 2 cut(s) 595, 627
AsiGI ACCGGT 1 cut(s) 131
AspS9I GGNCC 4 cut(s) 416, 566, 865, 1415
AsuC2I CCSGG 2 cut(s) 84, 570
AsuHPI GGTGA 3 cut(s) 1081, 1592, 2246
AvaII GGWCC 3 cut(s) 416, 865, 1415
BaeGI GKGCMC 2 cut(s) 227, 1059
BaeI ACNNNNGTAYC 2 cut(s) 1990, 2023
BamHI GGATCC 1 cut(s) 1903
BanI GGYRCC 1 cut(s) 167
BauI CACGAG 1 cut(s) 2034
BbsI GAAGAC 1 cut(s) 2172
Bbv12I GWGCWC 3 cut(s) 227, 370, 657
BbvI GCAGC 3 cut(s) 166, 334, 1487
BccI CCATC 6 cut(s) 415, 692, 704, 1265, 1437, 2225
BceAI ACGGC 1 cut(s) 580
BciVI GTATCC 1 cut(s) 1254
BcnI CCSGG 2 cut(s) 84, 570
BcoDI GTCTC 1 cut(s) 1351
BcuI ACTAGT 2 cut(s) 32, 587
BfaI CTAG 8 cut(s) 21, 33, 305, 588, 753, 1574, 2109, 2243
BfuI GTATCC 1 cut(s) 1254
BglII AGATCT 1 cut(s) 1951
BisI GCNGC 5 cut(s) 155, 348, 540, 957, 1476
BlpI GCTNAGC 1 cut(s) 1029
BlsI GCNGC 5 cut(s) 156, 349, 541, 958, 1477
BmcAI AGTACT 2 cut(s) 25, 98
Bme1390I CCNGG 2 cut(s) 84, 570
Bme18I GGWCC 3 cut(s) 416, 865, 1415
BmgT120I GGNCC 4 cut(s) 416, 566, 865, 1415
BmiI GGNNCC 5 cut(s) 169, 300, 568, 1286, 1905
BmrFI CCNGG 2 cut(s) 84, 570
BmsI GCATC 8 cut(s) 307, 671, 772, 794, 804, 1211, 1228, 1560
BpiI GAAGAC 1 cut(s) 2172
Bpu10I CCTNAGC 1 cut(s) 599
Bpu1102I GCTNAGC 1 cut(s) 1029
BpuEI CTTGAG 2 cut(s) 1778, 1955
BpuMI CCSGG 2 cut(s) 84, 570
Bsa29I ATCGAT 1 cut(s) 1845
BsaBI GATNNNNATC 1 cut(s) 2211
BsaI GGTCTC 1 cut(s) 1351
BsaJI CCNNGG 4 cut(s) 83, 558, 568, 2144
BsaWI WCCGGW 3 cut(s) 131, 1705, 1906
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 383, 1746
Bse118I RCCGGY 1 cut(s) 131
Bse3DI GCAATG 4 cut(s) 339, 961, 1466, 2169
Bse8I GATNNNNATC 1 cut(s) 2211
BseCI ATCGAT 1 cut(s) 1845
BseDI CCNNGG 4 cut(s) 83, 558, 568, 2144
BseGI GGATG 8 cut(s) 686, 703, 715, 1226, 1575, 2182, 2236, 2260
BseJI GATNNNNATC 1 cut(s) 2211
BseLI CCNNNNNNNGG 3 cut(s) 83, 383, 1746
BseMI GCAATG 4 cut(s) 339, 961, 1466, 2169
BseRI GAGGAG 6 cut(s) 857, 869, 1441, 2022, 2037, 2040
BseSI GKGCMC 2 cut(s) 227, 1059
BseXI GCAGC 3 cut(s) 166, 334, 1487
BshFI GGCC 3 cut(s) 70, 567, 1655
BshNI GGYRCC 1 cut(s) 167
BshTI ACCGGT 1 cut(s) 131
BshVI ATCGAT 1 cut(s) 1845
BsiHKAI GWGCWC 3 cut(s) 227, 370, 657
BsiSI CCGG 6 cut(s) 83, 132, 570, 1115, 1706, 1907
BslFI GGGAC 3 cut(s) 52, 185, 1708
BslI CCNNNNNNNGG 3 cut(s) 83, 383, 1746
BsmAI GTCTC 1 cut(s) 1351
BsmFI GGGAC 3 cut(s) 52, 185, 1708
BsmI GAATGC 1 cut(s) 506
BsnI GGCC 3 cut(s) 70, 567, 1655
Bso31I GGTCTC 1 cut(s) 1351
Bsp1286I GDGCHC 4 cut(s) 227, 370, 657, 1059
Bsp143I GATC 8 cut(s) 52, 1585, 1676, 1842, 1846, 1903, 1921, 1951
Bsp1720I GCTNAGC 1 cut(s) 1029
BspACI CCGC 8 cut(s) 539, 956, 1118, 1361, 1478, 1598, 1973, 2174
BspANI GGCC 3 cut(s) 70, 567, 1655
BspDI ATCGAT 1 cut(s) 1845
BspHI TCATGA 2 cut(s) 592, 1267
BspLI GGNNCC 5 cut(s) 169, 300, 568, 1286, 1905
BspPI GGATC 5 cut(s) 1593, 1841, 1898, 1911, 1916
BspT107I GGYRCC 1 cut(s) 167
BspTNI GGTCTC 1 cut(s) 1351
BsrDI GCAATG 4 cut(s) 339, 961, 1466, 2169
BsrFI RCCGGY 1 cut(s) 131
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 4 cut(s) 83, 558, 568, 2144
BssMI GATC 8 cut(s) 52, 1585, 1676, 1842, 1846, 1903, 1921, 1951
BssSI CACGAG 1 cut(s) 2034
BssT1I CCWWGG 2 cut(s) 558, 2144
Bst2BI CACGAG 1 cut(s) 2034
Bst4CI ACNGT 1 cut(s) 274
Bst6I CTCTTC 4 cut(s) 246, 303, 1155, 1548
BstC8I GCNNGC 3 cut(s) 453, 1473, 1776
BstDEI CTNAG 5 cut(s) 599, 1029, 1383, 1808, 1821
BstF5I GGATG 8 cut(s) 686, 703, 715, 1226, 1575, 2182, 2236, 2260
BstKTI GATC 8 cut(s) 55, 1588, 1679, 1845, 1849, 1906, 1924, 1954
BstMAI GTCTC 1 cut(s) 1351
BstMBI GATC 8 cut(s) 52, 1585, 1676, 1842, 1846, 1903, 1921, 1951
BstMWI GCNNNNNNNGC 2 cut(s) 452, 1540
BstNSI RCATGY 3 cut(s) 1475, 1538, 1778
BstSCI CCNGG 2 cut(s) 82, 568
BstSLI GKGCMC 2 cut(s) 227, 1059
BstV1I GCAGC 3 cut(s) 166, 334, 1487
BstV2I GAAGAC 1 cut(s) 2172
BstX2I RGATCY 2 cut(s) 1903, 1951
BstYI RGATCY 2 cut(s) 1903, 1951
Bsu15I ATCGAT 1 cut(s) 1845
BsuI GTATCC 1 cut(s) 1254
BsuRI GGCC 3 cut(s) 70, 567, 1655
BsuTUI ATCGAT 1 cut(s) 1845
BtsCI GGATG 8 cut(s) 686, 703, 715, 1226, 1575, 2182, 2236, 2260
Cac8I GCNNGC 3 cut(s) 453, 1473, 1776
CciI TCATGA 2 cut(s) 592, 1267
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 4 cut(s) 416, 566, 865, 1415
ClaI ATCGAT 1 cut(s) 1845
CseI GACGC 1 cut(s) 2147
Csp6I GTAC 5 cut(s) 24, 97, 759, 1995, 2007
CspAI ACCGGT 1 cut(s) 131
CviQI GTAC 5 cut(s) 24, 97, 759, 1995, 2007
DdeI CTNAG 5 cut(s) 599, 1029, 1383, 1808, 1821
DpnI GATC 8 cut(s) 54, 1587, 1678, 1844, 1848, 1905, 1923, 1953
DpnII GATC 8 cut(s) 52, 1585, 1676, 1842, 1846, 1903, 1921, 1951
DraIII CACNNNGTG 1 cut(s) 383
Eam1104I CTCTTC 4 cut(s) 246, 303, 1155, 1548
EarI CTCTTC 4 cut(s) 246, 303, 1155, 1548
Eco130I CCWWGG 2 cut(s) 558, 2144
Eco31I GGTCTC 1 cut(s) 1351
Eco47I GGWCC 3 cut(s) 416, 865, 1415
EcoRI GAATTC 1 cut(s) 463
EcoT14I CCWWGG 2 cut(s) 558, 2144
EcoT22I ATGCAT 1 cut(s) 787
ErhI CCWWGG 2 cut(s) 558, 2144
FaqI GGGAC 3 cut(s) 52, 185, 1708
FauI CCCGC 1 cut(s) 1471
FauNDI CATATG 1 cut(s) 2200
Fnu4HI GCNGC 5 cut(s) 155, 348, 540, 957, 1476
FokI GGATG 8 cut(s) 693, 710, 722, 1233, 1582, 2189, 2243, 2267
Fsp4HI GCNGC 5 cut(s) 155, 348, 540, 957, 1476
FspBI CTAG 8 cut(s) 21, 33, 305, 588, 753, 1574, 2109, 2243
GluI GCNGC 5 cut(s) 155, 348, 540, 957, 1476
HaeIII GGCC 3 cut(s) 70, 567, 1655
HapII CCGG 6 cut(s) 83, 132, 570, 1115, 1706, 1907
HgaI GACGC 1 cut(s) 2147
HincII GTYRAC 1 cut(s) 401
HindII GTYRAC 1 cut(s) 401
HindIII AAGCTT 2 cut(s) 186, 2138
HinfI GANTC 8 cut(s) 180, 397, 946, 1346, 1387, 1447, 1732, 1871
HpaII CCGG 6 cut(s) 83, 132, 570, 1115, 1706, 1907
HphI GGTGA 3 cut(s) 1081, 1592, 2246
Hpy166II GTNNAC 4 cut(s) 225, 401, 945, 1451
Hpy188I TCNGA 4 cut(s) 697, 790, 1351, 1876
Hpy188III TCNNGA 6 cut(s) 475, 593, 1268, 2036, 2075, 2162
Hpy8I GTNNAC 4 cut(s) 225, 401, 945, 1451
HpyAV CCTTC 3 cut(s) 208, 610, 2079
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4IV ACGT 1 cut(s) 490
HpyF10VI GCNNNNNNNGC 2 cut(s) 452, 1540
HpyF3I CTNAG 5 cut(s) 599, 1029, 1383, 1808, 1821
HpySE526I ACGT 1 cut(s) 490
Kzo9I GATC 8 cut(s) 52, 1585, 1676, 1842, 1846, 1903, 1921, 1951
LmnI GCTCC 2 cut(s) 298, 547
LpnPI CCDG 8 cut(s) 96, 145, 336, 583, 1128, 1529, 1719, 1920
Lsp1109I GCAGC 3 cut(s) 166, 334, 1487
LweI GCATC 8 cut(s) 307, 671, 772, 794, 804, 1211, 1228, 1560
MaeI CTAG 8 cut(s) 21, 33, 305, 588, 753, 1574, 2109, 2243
MaeII ACGT 1 cut(s) 490
MaeIII GTNAC 3 cut(s) 7, 874, 1120
MalI GATC 8 cut(s) 54, 1587, 1678, 1844, 1848, 1905, 1923, 1953
MboI GATC 8 cut(s) 52, 1585, 1676, 1842, 1846, 1903, 1921, 1951
MfeI CAATTG 1 cut(s) 1989
MflI RGATCY 2 cut(s) 1903, 1951
MhlI GDGCHC 4 cut(s) 227, 370, 657, 1059
MlyI GAGTC 3 cut(s) 406, 940, 1456
MmeI TCCRAC 4 cut(s) 608, 675, 683, 960
Mph1103I ATGCAT 1 cut(s) 787
MseI TTAA 3 cut(s) 237, 798, 1593
MslI CAYNNNNRTG 3 cut(s) 689, 1187, 1236
MspA1I CMGCKG 1 cut(s) 1478
MspI CCGG 6 cut(s) 83, 132, 570, 1115, 1706, 1907
MspR9I CCNGG 2 cut(s) 84, 570
MunI CAATTG 1 cut(s) 1989
Mva1269I GAATGC 1 cut(s) 506
MwoI GCNNNNNNNGC 2 cut(s) 452, 1540
NciI CCSGG 2 cut(s) 84, 570
NdeI CATATG 1 cut(s) 2200
NdeII GATC 8 cut(s) 52, 1585, 1676, 1842, 1846, 1903, 1921, 1951
NlaIV GGNNCC 5 cut(s) 169, 300, 568, 1286, 1905
NmeAIII GCCGAG 1 cut(s) 427
NsiI ATGCAT 1 cut(s) 787
NspI RCATGY 3 cut(s) 1475, 1538, 1778
PaeI GCATGC 2 cut(s) 1475, 1778
PagI TCATGA 2 cut(s) 592, 1267
PcsI WCGNNNNNNNCGW 1 cut(s) 401
PctI GAATGC 1 cut(s) 506
PfeI GAWTC 5 cut(s) 180, 1346, 1387, 1732, 1871
PflMI CCANNNNNTGG 1 cut(s) 383
PinAI ACCGGT 1 cut(s) 131
PkrI GCNGC 5 cut(s) 156, 349, 541, 958, 1477
PleI GAGTC 3 cut(s) 405, 940, 1455
PpsI GAGTC 3 cut(s) 405, 940, 1455
PsiI TTATAA 1 cut(s) 897
PspN4I GGNNCC 5 cut(s) 169, 300, 568, 1286, 1905
PspPI GGNCC 4 cut(s) 416, 566, 865, 1415
PsuI RGATCY 2 cut(s) 1903, 1951
RsaI GTAC 5 cut(s) 25, 98, 760, 1996, 2008
RsaNI GTAC 5 cut(s) 24, 97, 759, 1995, 2007
RseI CAYNNNNRTG 3 cut(s) 689, 1187, 1236
SaqAI TTAA 3 cut(s) 237, 798, 1593
SatI GCNGC 5 cut(s) 155, 348, 540, 957, 1476
Sau3AI GATC 8 cut(s) 52, 1585, 1676, 1842, 1846, 1903, 1921, 1951
Sau96I GGNCC 4 cut(s) 416, 566, 865, 1415
ScaI AGTACT 2 cut(s) 25, 98
SchI GAGTC 3 cut(s) 406, 940, 1456
ScrFI CCNGG 2 cut(s) 84, 570
SduI GDGCHC 4 cut(s) 227, 370, 657, 1059
SfaNI GCATC 8 cut(s) 307, 671, 772, 794, 804, 1211, 1228, 1560
SinI GGWCC 3 cut(s) 416, 865, 1415
SmiMI CAYNNNNRTG 3 cut(s) 689, 1187, 1236
SmlI CTYRAG 2 cut(s) 1757, 1934
SmoI CTYRAG 2 cut(s) 1757, 1934
SpeI ACTAGT 2 cut(s) 32, 587
SphI GCATGC 2 cut(s) 1475, 1778
SsiI CCGC 8 cut(s) 539, 956, 1118, 1361, 1478, 1598, 1973, 2174
SspMI CTAG 8 cut(s) 21, 33, 305, 588, 753, 1574, 2109, 2243
StyD4I CCNGG 2 cut(s) 82, 568
StyI CCWWGG 2 cut(s) 558, 2144
TaaI ACNGT 1 cut(s) 274
TaiI ACGT 1 cut(s) 493
TaqI TCGA 3 cut(s) 193, 1683, 1845
TaqII GACCGA 1 cut(s) 433
TatI WGTACW 2 cut(s) 23, 96
TauI GCSGC 2 cut(s) 542, 959
TfiI GAWTC 5 cut(s) 180, 1346, 1387, 1732, 1871
Tru1I TTAA 3 cut(s) 237, 798, 1593
Tru9I TTAA 3 cut(s) 237, 798, 1593
TseI GCWGC 3 cut(s) 154, 347, 1475
TspGWI ACGGA 2 cut(s) 722, 1907
Van91I CCANNNNNTGG 1 cut(s) 383
VneI GTGCAC 1 cut(s) 223
VpaK11BI GGWCC 3 cut(s) 416, 865, 1415
XapI RAATTY 5 cut(s) 463, 664, 733, 1085, 1276
XceI RCATGY 3 cut(s) 1475, 1538, 1778
XspI CTAG 8 cut(s) 21, 33, 305, 588, 753, 1574, 2109, 2243
ZrmI AGTACT 2 cut(s) 25, 98
Zsp2I ATGCAT 1 cut(s) 787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.