pycom16g16790

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002052
Physical Location & Seq
Reverse (-)
12801813 .. 12802720
908 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g16790.1

Sequence Viewer

Length: 558 bp
ATGGAGAGGAAAGAGAAGGTGTGGTGGACTCCATGTGCGGCTCATTGCATTGATTTGATGCTAGAAGATATTTCTAAGATGGCATGGTTTGATGACACACTCAAACGTGCTAGGTGTATTTCAAAGTATCTATATGGGCATCAATGGGTACTAGCTTTGATGAGAAAGTACACTAACAATTCGGAAATTCTTCGTCCGGTGGTCACTAGGTTTGCCACTTCTTTCCTTACACTACAAAGCTTACAAAAGCAAAAGGAAAATCTTGTTGCTTTGTTTACCTCTCAAGAATGGTCGGAAAGTACCTATGCAAAATCCCGTGAAGCAAAGTTGGCTAAGCATCATGTGTTATATGATCATACTGATGAAGTGCGAAATGGGTTGTTTGCTTGCATGGATAGGATGCTTGGGAAAGGGAAAGAACGTGAAGAGGCCGATGTTCAATTGGATTTATTTACTAATAAGCGTGGTCCATTTGCGGATTCTATGGCTATGCAAACTAGGAAAAAGCGAACACCAAATTGTTTTTTTAGCGGCTTGGTGGGAGCGCTTTGGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

186

Amino Acids

21.59

Weight (kDa)

9.21

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 1 - 40 3.5e-11 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 38, 476, 531
AcsI RAATTY 1 cut(s) 186
AfaI GTAC 3 cut(s) 150, 170, 301
AfeI AGCGCT 1 cut(s) 546
AgsI TTSAA 2 cut(s) 123, 440
AluBI AGCT 2 cut(s) 155, 240
AluI AGCT 2 cut(s) 155, 240
Aor51HI AGCGCT 1 cut(s) 546
AoxI GGCC 1 cut(s) 429
ApoI RAATTY 1 cut(s) 186
Asp700I GAANNNNTTC 1 cut(s) 189
AspLEI GCGC 1 cut(s) 547
AspS9I GGNCC 1 cut(s) 467
AvaII GGWCC 1 cut(s) 467
BccI CCATC 1 cut(s) 73
BclI TGATCA 1 cut(s) 352
BfaI CTAG 5 cut(s) 62, 111, 152, 207, 498
BfoI RGCGCY 1 cut(s) 548
BisI GCNGC 2 cut(s) 39, 532
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 2 cut(s) 40, 533
Bme18I GGWCC 1 cut(s) 467
BmgT120I GGNCC 1 cut(s) 467
BmsI GCATC 4 cut(s) 48, 148, 346, 390
Bpu1102I GCTNAGC 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 267
BsaWI WCCGGW 1 cut(s) 196
Bse3DI GCAATG 1 cut(s) 43
BseGI GGATG 1 cut(s) 405
BseMI GCAATG 1 cut(s) 43
BshFI GGCC 1 cut(s) 431
BsiSI CCGG 1 cut(s) 197
BsnI GGCC 1 cut(s) 431
Bsp143I GATC 1 cut(s) 352
Bsp1720I GCTNAGC 1 cut(s) 333
BspACI CCGC 3 cut(s) 38, 476, 531
BspANI GGCC 1 cut(s) 431
BsrDI GCAATG 1 cut(s) 43
BssMI GATC 1 cut(s) 352
Bst6I CTCTTC 1 cut(s) 420
BstC8I GCNNGC 1 cut(s) 388
BstDEI CTNAG 2 cut(s) 75, 333
BstF5I GGATG 1 cut(s) 405
BstH2I RGCGCY 1 cut(s) 548
BstHHI GCGC 1 cut(s) 547
BstKTI GATC 1 cut(s) 355
BstMBI GATC 1 cut(s) 352
BstMWI GCNNNNNNNGC 2 cut(s) 329, 551
BsuRI GGCC 1 cut(s) 431
BtsCI GGATG 1 cut(s) 405
Cac8I GCNNGC 1 cut(s) 388
CfoI GCGC 1 cut(s) 547
Cfr13I GGNCC 1 cut(s) 467
Csp6I GTAC 3 cut(s) 149, 169, 300
CviAII CATG 4 cut(s) 33, 84, 341, 391
CviJI RGCY 7 cut(s) 41, 155, 240, 332, 431, 488, 534
CviKI_1 RGCY 7 cut(s) 41, 155, 240, 332, 431, 488, 534
CviQI GTAC 3 cut(s) 149, 169, 300
DdeI CTNAG 2 cut(s) 75, 333
DpnI GATC 1 cut(s) 354
DpnII GATC 1 cut(s) 352
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
Eco47I GGWCC 1 cut(s) 467
Eco47III AGCGCT 1 cut(s) 546
FaeI CATG 4 cut(s) 36, 87, 344, 394
FatI CATG 4 cut(s) 32, 83, 340, 390
FbaI TGATCA 1 cut(s) 352
Fnu4HI GCNGC 2 cut(s) 39, 532
FokI GGATG 1 cut(s) 412
Fsp4HI GCNGC 2 cut(s) 39, 532
FspBI CTAG 5 cut(s) 62, 111, 152, 207, 498
GlaI GCGC 1 cut(s) 546
GluI GCNGC 2 cut(s) 39, 532
HaeII RGCGCY 1 cut(s) 548
HaeIII GGCC 1 cut(s) 431
HapII CCGG 1 cut(s) 197
HhaI GCGC 1 cut(s) 547
Hin1II CATG 4 cut(s) 36, 87, 344, 394
Hin6I GCGC 1 cut(s) 545
HinP1I GCGC 1 cut(s) 545
HindIII AAGCTT 1 cut(s) 238
HinfI GANTC 2 cut(s) 28, 479
HpaII CCGG 1 cut(s) 197
Hpy166II GTNNAC 3 cut(s) 27, 171, 276
Hpy188I TCNGA 2 cut(s) 184, 295
Hpy188III TCNNGA 1 cut(s) 284
Hpy8I GTNNAC 3 cut(s) 27, 171, 276
HpyAV CCTTC 1 cut(s) 10
HpyCH4IV ACGT 2 cut(s) 106, 421
HpyCH4V TGCA 4 cut(s) 48, 308, 390, 493
HpyF10VI GCNNNNNNNGC 2 cut(s) 329, 551
HpyF3I CTNAG 2 cut(s) 75, 333
HpySE526I ACGT 2 cut(s) 106, 421
Hsp92II CATG 4 cut(s) 36, 87, 344, 394
HspAI GCGC 1 cut(s) 545
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 1 cut(s) 352
LmnI GCTCC 1 cut(s) 542
LpnPI CCDG 1 cut(s) 210
LweI GCATC 4 cut(s) 48, 148, 346, 390
MaeI CTAG 5 cut(s) 62, 111, 152, 207, 498
MaeII ACGT 2 cut(s) 106, 421
MaeIII GTNAC 1 cut(s) 202
MalI GATC 1 cut(s) 354
MboI GATC 1 cut(s) 352
MboII GAAGA 3 cut(s) 77, 182, 437
MfeI CAATTG 1 cut(s) 440
MluCI AATT 4 cut(s) 178, 186, 440, 517
MlyI GAGTC 1 cut(s) 22
MmeI TCCRAC 1 cut(s) 273
MnlI CCTC 2 cut(s) 289, 421
MroXI GAANNNNTTC 1 cut(s) 189
MslI CAYNNNNRTG 1 cut(s) 360
MspI CCGG 1 cut(s) 197
MunI CAATTG 1 cut(s) 440
MwoI GCNNNNNNNGC 2 cut(s) 329, 551
NdeII GATC 1 cut(s) 352
NlaIII CATG 4 cut(s) 36, 87, 344, 394
NmuCI GTSAC 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 189
PfeI GAWTC 1 cut(s) 479
PkrI GCNGC 2 cut(s) 40, 533
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
PspPI GGNCC 1 cut(s) 467
RsaI GTAC 3 cut(s) 150, 170, 301
RsaNI GTAC 3 cut(s) 149, 169, 300
RseI CAYNNNNRTG 1 cut(s) 360
SatI GCNGC 2 cut(s) 39, 532
Sau3AI GATC 1 cut(s) 352
Sau96I GGNCC 1 cut(s) 467
SchI GAGTC 1 cut(s) 22
SetI ASST 9 cut(s) 21, 109, 116, 157, 212, 242, 281, 305, 424
SfaNI GCATC 4 cut(s) 48, 148, 346, 390
SinI GGWCC 1 cut(s) 467
SmiMI CAYNNNNRTG 1 cut(s) 360
SmlI CTYRAG 1 cut(s) 282
SmoI CTYRAG 1 cut(s) 282
Sse9I AATT 4 cut(s) 178, 186, 440, 517
SsiI CCGC 3 cut(s) 38, 476, 531
SspMI CTAG 5 cut(s) 62, 111, 152, 207, 498
TaiI ACGT 2 cut(s) 109, 424
TasI AATT 4 cut(s) 178, 186, 440, 517
TatI WGTACW 1 cut(s) 168
TauI GCSGC 2 cut(s) 41, 534
TfiI GAWTC 1 cut(s) 479
TseFI GTSAC 1 cut(s) 202
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 1 cut(s) 378
VpaK11BI GGWCC 1 cut(s) 467
XapI RAATTY 1 cut(s) 186
XmnI GAANNNNTTC 1 cut(s) 189
XspI CTAG 5 cut(s) 62, 111, 152, 207, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.