Rw0G004680

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00175
OG0002052
Physical Location & Seq
Forward (+)
9217 .. 18192
8976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G004680.1

Sequence Viewer

Length: 2355 bp
ATGTGGTTGGTAATTGGATTAGCCATGAGTGAGAAAGGGGAAGATGGAAGAGACAAAGCATGGAAGTACACACAAAAATTGGAAGGGGAGAGATACTTTAGATGTGTATTTTGTAATCAAGTATGCAGTGGCACTATATCTAGACTTAAGCATCATTTAGCAGGTGTTGCGGGTGGTATGAAAGCATGTAATAAAGTCCCTGCAGATGTGAAGAAAGAGTACAAATTAGTTTTAAAAGTATTTAAAGATGATAAAAATAAGAGAAGTATTATGTTGAGTGAGATTGGGGAAGGAATTGGTGTAGGAAGTAGTAGTTTTGATTCTCCATTAAGTGAAGAATGTGGGAGTAACCCAAGTAATACTACAGCTCAACCAAAAACTAAAGGACCACTTGATAGATTTGTGTCTTCGGAGCCTAGGCAAACCGTATTGAATGAGGCTTACAAAAAAGAATTAAGTAAAGAAGTTTGTCGAATGGTTGGGCGTTTTATGTTTTCTAGAGTGCTTCCTTTCAATACAGTTAATGATCCGTTTTGGTTAGCTATGGTAGAGGGAATTGCTAAGTATGGTGTAGGGTTCAAGCCCCCTTCTATGCATGAATTAAGGACTTGGATCCTTAGGGAAGAAGTTGAAGACATTGATAAGTATTTTTTAGTCCATAAAAAAGCTTGGGAAACATATGGGTGTTCCATTATGTCGGATGGTTGGACCGATGGAAAGGGTAGGGTGCTAATTAATTTTCTTGTGAATAGTCATGCGGGTAGTTTTTTTTTTAAGTCCATTGATGCTTCCGGTAGCATAAAAAATGGGTCTTTGATGTTGAAGTACTTGGATGATGTGGTGAGAGAAGTAGGGGAAGAGAATGTGGTTCAAATAATCACCGATAATGCATCCAATTATAAGAATGCCGGGATTAGGCTTATGCAAAGAAGAGAAAGGTTGTGGTGGACTCCATGTGCAGCTCATTGTATTGATCTTATGCTTGAAGATATTAGCAAAATAACGGTGTATGAGAAAACAATTAAAAGTGCTAAGCAAGTGGTCAAGTTTATCTATGGTCACACATGGGTTCTTTCTCTCATGAGGGAATTCACTAGCAATAAGGAGATTATTCGTCCGGCCATCACTCTTTTTGCAACTTCCTTTCTCACCCTTCAAAGTATCTATAAGCAGAAGCAGCCTCTACAGATGATGTTTAACAGCAAGAAGTGGATTGAGGGCCCTTTTGTCAAAAGCCATGAAGCTATTCAGGTTCGTGCAATTGTTCTTTTTCAGAAGAATTTTTGGAGTGATGTTGCATTTTGTGTTAAGGGTGTGTTGCCATTAGTGTGTGTATTGAGGGAGGTTGATTCTGAGCTAAGACCTGTTATGGGCTACATATATGAATTGATGGATGCTGCCAAAGAGAAAATTGCATTTAGTTTGAAGAGGAACCCAAGACATTATCAGCCAATTTGGAATAAAATTGATGCAAGGTGGACTCCCCAACTCCATCAACCTTTACACGCTGCTGGATACTATCTGAACCCTCAATTTCATTATGAAGAGAACTTCTCAAATGTTTTGGAAGTGAAGAAGGGATTGCATGAATGCATGGATCGGATGATGTCGTTTGATGAGCGCTTAAAAGCTGATATCCAATTAAAGATGTTTGACAAATGCATGGGAGAGTTTGGAACTAGGCTTGCAATACATTCTAGAAAGATCAGGAGTCCAGCAAGTTGGTGGGAGCGTTTTGGAGAGGAAACGCCTGAATTGACAAAGTTTGCTATTCGTATTCACACAAAAAAAAGAAATAGACTTGAGCATAAAAGGCTAAATGCATTGGTTTATGTGAAGTACAACTCTTTACTAAGAGAGCGAAACATTAGAAGAAATACAAAGAAGCTTGATCCTATATTAGTGGAAGAAATTGATTCTGATGATGAATGGATTTCGGAGGTGGAAGATCCGGTTCTTCCGGGTGATCTTTCTTGGCTTGAAGATGATCTATTTGAGGTTGATGCTATAAGGAATGTGCCAATAGAATGTTATGAACAAGGTTTGTCAACTAGAGTGCCTATTCATGTAGAGCCACTAGAGGAGCCGATCTTAGATGATGAGCCTATCTTGGATGATGAGTTAGGATTTGATGAGCATGCTGCTTATTCACCTTCTCCTAAAAGAAAAATTGATGAAAGCTCAAGTAAATCCAAGAAGAAAATGAGAAAACTTGCTGCATTACTTAGGGATGAAGATGATGGGATAGAAGACCTCACTTTGTCTCCCCACACTGATGCAGCATTGGACATTGATGATTGTGATGATATTTATGTGGGTGTGGATGGATATGATGATTATGAATTGGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

784

Amino Acids

90.38

Weight (kDa)

6.17

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 196 - 347 1.6e-58 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 900
AarI CACCTGC 1 cut(s) 152
Acc36I ACCTGC 1 cut(s) 152
AciI CCGC 2 cut(s) 170, 758
AclWI GGATC 6 cut(s) 521, 607, 620, 1605, 1886, 1943
AcoI YGGCCR 1 cut(s) 1119
AcsI RAATTY 2 cut(s) 1088, 1279
AfaI GTAC 4 cut(s) 68, 221, 827, 1841
AfeI AGCGCT 1 cut(s) 1622
AfiI CCNNNNNNNGG 2 cut(s) 915, 1370
AflII CTTAAG 1 cut(s) 146
AluBI AGCT 9 cut(s) 368, 542, 668, 962, 1244, 1357, 1631, 1888, 2181
AluI AGCT 9 cut(s) 368, 542, 668, 962, 1244, 1357, 1631, 1888, 2181
Alw26I GTCTC 2 cut(s) 45, 2268
AlwI GGATC 6 cut(s) 521, 607, 620, 1605, 1886, 1943
Aor51HI AGCGCT 1 cut(s) 1622
AoxI GGCC 2 cut(s) 1119, 1219
ApaI GGGCCC 1 cut(s) 1223
ApeKI GCWGC 7 cut(s) 959, 1177, 1397, 1508, 2141, 2216, 2279
ApoI RAATTY 2 cut(s) 1088, 1279
AseI ATTAAT 1 cut(s) 735
Asp700I GAANNNNTTC 1 cut(s) 1245
AspA2I CCTAGG 1 cut(s) 416
AspLEI GCGC 1 cut(s) 1623
AspS9I GGNCC 4 cut(s) 386, 708, 1219, 1220
AsuC2I CCSGG 2 cut(s) 910, 1962
AsuHPI GGTGA 5 cut(s) 853, 871, 1141, 1976, 2142
AvaII GGWCC 2 cut(s) 386, 708
AvrII CCTAGG 1 cut(s) 416
AxyI CCTNAGG 1 cut(s) 617
BaeGI GKGCMC 1 cut(s) 1223
BamHI GGATCC 1 cut(s) 612
BanII GRGCYC 1 cut(s) 1223
BarI GAAGNNNNNNTAC 2 cut(s) 203, 235
BbsI GAAGAC 3 cut(s) 399, 639, 2256
BbvI GCAGC 7 cut(s) 971, 1189, 1384, 1495, 2128, 2203, 2291
BccI CCATC 8 cut(s) 38, 695, 707, 1130, 1384, 1500, 2234, 2318
BciVI GTATCC 1 cut(s) 1508
BcnI CCSGG 2 cut(s) 910, 1962
BcoDI GTCTC 2 cut(s) 45, 2268
BfaI CTAG 8 cut(s) 141, 417, 498, 1095, 1680, 1698, 2052, 2078
BfmI CTRYAG 3 cut(s) 201, 363, 1184
BfoI RGCGCY 1 cut(s) 1624
BfrI CTTAAG 1 cut(s) 146
BfuAI ACCTGC 1 cut(s) 152
BfuI GTATCC 1 cut(s) 1508
BisI GCNGC 7 cut(s) 960, 1178, 1398, 1509, 2142, 2217, 2280
BlnI CCTAGG 1 cut(s) 416
BlpI GCTNAGC 1 cut(s) 1032
BlsI GCNGC 7 cut(s) 961, 1179, 1399, 1510, 2143, 2218, 2281
BmcAI AGTACT 1 cut(s) 827
Bme1390I CCNGG 2 cut(s) 910, 1962
Bme18I GGWCC 2 cut(s) 386, 708
BmgT120I GGNCC 4 cut(s) 386, 708, 1219, 1220
BmiI GGNNCC 5 cut(s) 414, 614, 1221, 1433, 2085
BmrFI CCNGG 2 cut(s) 910, 1962
BmsI GCATC 7 cut(s) 160, 775, 899, 1384, 1459, 1993, 2266
BpiI GAAGAC 3 cut(s) 399, 639, 2256
BplI GAGNNNNNCTC 2 cut(s) 1538, 1570
Bpu1102I GCTNAGC 1 cut(s) 1032
BpuEI CTTGAG 2 cut(s) 1823, 2167
BpuMI CCSGG 2 cut(s) 910, 1962
BsaJI CCNNGG 1 cut(s) 416
BsaWI WCCGGW 2 cut(s) 791, 1951
BsaXI ACNNNNNCTCC 2 cut(s) 2248, 2278
Bsc4I CCNNNNNNNGG 2 cut(s) 915, 1370
Bse21I CCTNAGG 1 cut(s) 617
BseDI CCNNGG 1 cut(s) 416
BseGI GGATG 8 cut(s) 706, 838, 890, 1399, 1608, 2119, 2236, 2329
BseLI CCNNNNNNNGG 2 cut(s) 915, 1370
BseMII CTCAG 1 cut(s) 1344
BseRI GAGGAG 1 cut(s) 2096
BseSI GKGCMC 1 cut(s) 1223
BseXI GCAGC 7 cut(s) 971, 1189, 1384, 1495, 2128, 2203, 2291
BsgI GTGCAG 1 cut(s) 978
BshFI GGCC 2 cut(s) 1121, 1221
BsiSI CCGG 5 cut(s) 792, 909, 1118, 1952, 1961
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 2 cut(s) 915, 1370
BsmAI GTCTC 2 cut(s) 45, 2268
BsmFI GGGAC 1 cut(s) 182
BsmI GAATGC 2 cut(s) 910, 1595
BsnI GGCC 2 cut(s) 1121, 1221
Bsp120I GGGCCC 1 cut(s) 1219
Bsp1286I GDGCHC 1 cut(s) 1223
Bsp1720I GCTNAGC 1 cut(s) 1032
BspACI CCGC 2 cut(s) 170, 758
BspANI GGCC 2 cut(s) 1121, 1221
BspCNI CTCAG 1 cut(s) 1345
BspHI TCATGA 1 cut(s) 1080
BspLI GGNNCC 5 cut(s) 414, 614, 1221, 1433, 2085
BspMAI CTGCAG 1 cut(s) 205
BspMI ACCTGC 1 cut(s) 152
BspPI GGATC 6 cut(s) 521, 607, 620, 1605, 1886, 1943
BspTI CTTAAG 1 cut(s) 146
BssECI CCNNGG 1 cut(s) 416
BssT1I CCWWGG 1 cut(s) 416
Bst4CI ACNGT 3 cut(s) 427, 520, 1006
Bst6I CTCTTC 5 cut(s) 43, 852, 925, 1421, 1539
BstAFI CTTAAG 1 cut(s) 146
BstAPI GCANNNNNTGC 1 cut(s) 167
BstC8I GCNNGC 2 cut(s) 1686, 2139
BstDEI CTNAG 8 cut(s) 561, 617, 1032, 1353, 1358, 1853, 2092, 2225
BstF5I GGATG 8 cut(s) 706, 838, 890, 1399, 1608, 2119, 2236, 2329
BstH2I RGCGCY 1 cut(s) 1624
BstHHI GCGC 1 cut(s) 1623
BstMAI GTCTC 2 cut(s) 45, 2268
BstMWI GCNNNNNNNGC 3 cut(s) 167, 1177, 1813
BstNSI RCATGY 2 cut(s) 189, 2141
BstSCI CCNGG 2 cut(s) 908, 1960
BstSFI CTRYAG 3 cut(s) 201, 363, 1184
BstSLI GKGCMC 1 cut(s) 1223
BstV1I GCAGC 7 cut(s) 971, 1189, 1384, 1495, 2128, 2203, 2291
BstV2I GAAGAC 3 cut(s) 399, 639, 2256
BstX2I RGATCY 2 cut(s) 612, 1948
BstXI CCANNNNNNTGG 1 cut(s) 1722
BstYI RGATCY 2 cut(s) 612, 1948
Bsu36I CCTNAGG 1 cut(s) 617
BsuI GTATCC 1 cut(s) 1508
BsuRI GGCC 2 cut(s) 1121, 1221
BtsCI GGATG 8 cut(s) 706, 838, 890, 1399, 1608, 2119, 2236, 2329
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 2 cut(s) 133, 2271
BveI ACCTGC 1 cut(s) 152
Cac8I GCNNGC 2 cut(s) 1686, 2139
CciI TCATGA 1 cut(s) 1080
CfoI GCGC 1 cut(s) 1623
Cfr13I GGNCC 4 cut(s) 386, 708, 1219, 1220
Csp6I GTAC 4 cut(s) 67, 220, 826, 1840
CviQI GTAC 4 cut(s) 67, 220, 826, 1840
DdeI CTNAG 8 cut(s) 561, 617, 1032, 1353, 1358, 1853, 2092, 2225
DraI TTTAAA 2 cut(s) 234, 244
EaeI YGGCCR 1 cut(s) 1119
Eam1104I CTCTTC 5 cut(s) 43, 852, 925, 1421, 1539
EarI CTCTTC 5 cut(s) 43, 852, 925, 1421, 1539
Eco130I CCWWGG 1 cut(s) 416
Eco24I GRGCYC 1 cut(s) 1223
Eco32I GATATC 1 cut(s) 1636
Eco47I GGWCC 2 cut(s) 386, 708
Eco47III AGCGCT 1 cut(s) 1622
Eco81I CCTNAGG 1 cut(s) 617
EcoO109I RGGNCCY 2 cut(s) 1219, 1220
EcoRI GAATTC 1 cut(s) 1088
EcoRV GATATC 1 cut(s) 1636
EcoT14I CCWWGG 1 cut(s) 416
EcoT22I ATGCAT 5 cut(s) 597, 892, 1595, 1664, 1825
EcoT38I GRGCYC 1 cut(s) 1223
ErhI CCWWGG 1 cut(s) 416
FalI AAGNNNNNCTT 2 cut(s) 375, 407
FaqI GGGAC 1 cut(s) 182
FauI CCCGC 2 cut(s) 163, 751
FauNDI CATATG 1 cut(s) 679
Fnu4HI GCNGC 7 cut(s) 960, 1178, 1398, 1509, 2142, 2217, 2280
FokI GGATG 8 cut(s) 713, 845, 877, 1406, 1615, 2126, 2243, 2336
FriOI GRGCYC 1 cut(s) 1223
Fsp4HI GCNGC 7 cut(s) 960, 1178, 1398, 1509, 2142, 2217, 2280
FspBI CTAG 8 cut(s) 141, 417, 498, 1095, 1680, 1698, 2052, 2078
GlaI GCGC 1 cut(s) 1622
GluI GCNGC 7 cut(s) 960, 1178, 1398, 1509, 2142, 2217, 2280
HaeII RGCGCY 1 cut(s) 1624
HaeIII GGCC 2 cut(s) 1121, 1221
HapII CCGG 5 cut(s) 792, 909, 1118, 1952, 1961
HhaI GCGC 1 cut(s) 1623
Hin6I GCGC 1 cut(s) 1621
HinP1I GCGC 1 cut(s) 1621
HincII GTYRAC 1 cut(s) 2049
HindII GTYRAC 1 cut(s) 2049
HindIII AAGCTT 2 cut(s) 666, 1886
HinfI GANTC 6 cut(s) 320, 949, 1349, 1480, 1711, 1916
HpaII CCGG 5 cut(s) 792, 909, 1118, 1952, 1961
HphI GGTGA 5 cut(s) 853, 871, 1141, 1976, 2142
Hpy166II GTNNAC 4 cut(s) 69, 948, 1479, 2049
Hpy188I TCNGA 8 cut(s) 412, 700, 1275, 1354, 1524, 1602, 1921, 1939
Hpy188III TCNNGA 5 cut(s) 141, 498, 1081, 1698, 1708
Hpy8I GTNNAC 4 cut(s) 69, 948, 1479, 2049
HpyAV CCTTC 6 cut(s) 77, 284, 597, 1163, 1570, 2163
HpyCH4III ACNGT 3 cut(s) 427, 520, 1006
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 1177, 1813
HpyF3I CTNAG 8 cut(s) 561, 617, 1032, 1353, 1358, 1853, 2092, 2225
HspAI GCGC 1 cut(s) 1621
LmnI GCTCC 3 cut(s) 412, 1729, 2083
Lsp1109I GCAGC 7 cut(s) 971, 1189, 1384, 1495, 2128, 2203, 2291
LweI GCATC 7 cut(s) 160, 775, 899, 1384, 1459, 1993, 2266
MaeI CTAG 8 cut(s) 141, 417, 498, 1095, 1680, 1698, 2052, 2078
MaeIII GTNAC 2 cut(s) 347, 1058
MfeI CAATTG 1 cut(s) 1260
MflI RGATCY 2 cut(s) 612, 1948
MhlI GDGCHC 1 cut(s) 1223
MlyI GAGTC 3 cut(s) 943, 1474, 1720
MmeI TCCRAC 2 cut(s) 678, 686
Mph1103I ATGCAT 5 cut(s) 597, 892, 1595, 1664, 1825
MroXI GAANNNNTTC 1 cut(s) 1245
MslI CAYNNNNRTG 3 cut(s) 682, 1327, 2274
MspCI CTTAAG 1 cut(s) 146
MspI CCGG 5 cut(s) 792, 909, 1118, 1952, 1961
MspR9I CCNGG 2 cut(s) 910, 1962
MunI CAATTG 1 cut(s) 1260
Mva1269I GAATGC 2 cut(s) 910, 1595
MwoI GCNNNNNNNGC 3 cut(s) 167, 1177, 1813
NciI CCSGG 2 cut(s) 910, 1962
NdeI CATATG 1 cut(s) 679
NlaIV GGNNCC 5 cut(s) 414, 614, 1221, 1433, 2085
NmuCI GTSAC 1 cut(s) 1058
NsiI ATGCAT 5 cut(s) 597, 892, 1595, 1664, 1825
NspI RCATGY 2 cut(s) 189, 2141
PaeI GCATGC 1 cut(s) 2141
PagI TCATGA 1 cut(s) 1080
PaqCI CACCTGC 1 cut(s) 152
PctI GAATGC 2 cut(s) 910, 1595
PdmI GAANNNNTTC 1 cut(s) 1245
PfeI GAWTC 3 cut(s) 320, 1349, 1916
PkrI GCNGC 7 cut(s) 961, 1179, 1399, 1510, 2143, 2218, 2281
PleI GAGTC 3 cut(s) 943, 1474, 1719
PpsI GAGTC 3 cut(s) 943, 1474, 1719
PshBI ATTAAT 1 cut(s) 735
PsiI TTATAA 1 cut(s) 900
PspN4I GGNNCC 5 cut(s) 414, 614, 1221, 1433, 2085
PspOMI GGGCCC 1 cut(s) 1219
PspPI GGNCC 4 cut(s) 386, 708, 1219, 1220
PstI CTGCAG 1 cut(s) 205
PsuI RGATCY 2 cut(s) 612, 1948
RsaI GTAC 4 cut(s) 68, 221, 827, 1841
RsaNI GTAC 4 cut(s) 67, 220, 826, 1840
RseI CAYNNNNRTG 3 cut(s) 682, 1327, 2274
SatI GCNGC 7 cut(s) 960, 1178, 1398, 1509, 2142, 2217, 2280
Sau96I GGNCC 4 cut(s) 386, 708, 1219, 1220
ScaI AGTACT 1 cut(s) 827
SchI GAGTC 3 cut(s) 943, 1474, 1720
ScrFI CCNGG 2 cut(s) 910, 1962
SduI GDGCHC 1 cut(s) 1223
SfaNI GCATC 7 cut(s) 160, 775, 899, 1384, 1459, 1993, 2266
SfcI CTRYAG 3 cut(s) 201, 363, 1184
SinI GGWCC 2 cut(s) 386, 708
SmiMI CAYNNNNRTG 3 cut(s) 682, 1327, 2274
SmlI CTYRAG 3 cut(s) 146, 1802, 2182
SmoI CTYRAG 3 cut(s) 146, 1802, 2182
SphI GCATGC 1 cut(s) 2141
SsiI CCGC 2 cut(s) 170, 758
SspMI CTAG 8 cut(s) 141, 417, 498, 1095, 1680, 1698, 2052, 2078
StyD4I CCNGG 2 cut(s) 908, 1960
StyI CCWWGG 1 cut(s) 416
TaaI ACNGT 3 cut(s) 427, 520, 1006
TaqI TCGA 1 cut(s) 472
TaqII GACCGA 1 cut(s) 725
TatI WGTACW 4 cut(s) 66, 219, 825, 1839
TfiI GAWTC 3 cut(s) 320, 1349, 1916
TscAI CASTG 2 cut(s) 133, 2278
TseFI GTSAC 1 cut(s) 1058
TseI GCWGC 7 cut(s) 959, 1177, 1397, 1508, 2141, 2216, 2279
Tsp45I GTSAC 1 cut(s) 1058
TspGWI ACGGA 1 cut(s) 519
TspRI CASTG 2 cut(s) 133, 2278
Vha464I CTTAAG 1 cut(s) 146
VpaK11BI GGWCC 2 cut(s) 386, 708
VspI ATTAAT 1 cut(s) 735
XapI RAATTY 2 cut(s) 1088, 1279
XbaI TCTAGA 3 cut(s) 140, 497, 1697
XceI RCATGY 2 cut(s) 189, 2141
XcmI CCANNNNNNNNNTGG 1 cut(s) 1722
XmaJI CCTAGG 1 cut(s) 416
XmnI GAANNNNTTC 1 cut(s) 1245
XspI CTAG 8 cut(s) 141, 417, 498, 1095, 1680, 1698, 2052, 2078
ZrmI AGTACT 1 cut(s) 827
Zsp2I ATGCAT 5 cut(s) 597, 892, 1595, 1664, 1825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.