FvH4_3g39520

Protein FLC EXPRESSOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000946
Physical Location & Seq
Reverse (-)
33598889 .. 33601324
2436 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g39520.t3

Sequence Viewer

Length: 780 bp
ATGGCCGGAAGAGAGCCTCGGCATCTCCACCGCCTCCCTCAAATCGTCAACACGACGGCCCTCGAGGACCGCGTCGATCTCCAGCAGCGCGAGATCCAGTCCCTCCTCATAGACAACCAACGCCTCGCGGCCACACACGTGGCGCTCAAGCAGGACCTGACTTCGGCGCAGAGCGACCTCCGCAACCTGAAGGCGGTGGCCGGCCAAATCAAATCCGAGAGGAACGCGGAAGTGCGGGAGGTATACGACAGGTCGTTGAAGCTGGACGACGAGCTCCGCGGCCTTGACGCCATGAAGGCCGAGCTGAGTGTGGTCCTGAACGACATCGAGGACTTGAGCGCTTCAAGGATGGAGCTCGCGACCGAGTTGAAGACGATCCAAGGAGAGATTGAGAGGACTAGGTCCGACGAGTCGAAGCAATTCGAAGCCAGACTGGACGAAATTAAGACATTGAAGGTGGAGATTGAGAAAGGAAGGGCTGCGATTGAGATTGAGAAGAAAACAAGAGCCAGTAACCTTGTGCATCGTCAAGCTATGGAGGACTATATGGCTGCGTTGGCTCTCGAAATCAAAAAGCTTCATGGGGAGTTGGCAAATGCGGAGAAGAGAGCAAGGGCTGCAGTAGCAGCAGCAAATCCAGTTGCAGGTTCCGGGTACCCTATGGCTTATGGCAATGCTGAAATGCTCTACGGAGGAAATGCATACCCTGACCCTTATATTGTGCATCAGGAACACTGCCCAAATTACTACAAGGTGCTAAGCTATTTGGAAAAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

260

Amino Acids

29.12

Weight (kDa)

5.88

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 62
Acc36I ACCTGC 1 cut(s) 635
Acc65I GGTACC 1 cut(s) 654
AccB1I GGYRCC 1 cut(s) 654
AccI GTMKAC 1 cut(s) 243
AccII CGCG 6 cut(s) 72, 90, 128, 227, 279, 359
AclWI GGATC 2 cut(s) 88, 370
AcoI YGGCCR 4 cut(s) 3, 129, 198, 202
AcuI CTGAAG 1 cut(s) 209
AcvI CACGTG 1 cut(s) 139
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 1 cut(s) 656
AfeI AGCGCT 1 cut(s) 340
AfiI CCNNNNNNNGG 3 cut(s) 163, 193, 644
AflIII ACRYGT 1 cut(s) 136
AgsI TTSAA 4 cut(s) 259, 345, 370, 454
AleI CACNNNNGTG 1 cut(s) 137
AluBI AGCT 7 cut(s) 262, 274, 304, 355, 533, 577, 762
AluI AGCT 7 cut(s) 262, 274, 304, 355, 533, 577, 762
Alw21I GWGCWC 2 cut(s) 276, 357
AlwI GGATC 2 cut(s) 88, 370
AlwNI CAGNNNCTG 1 cut(s) 157
Ama87I CYCGRG 1 cut(s) 62
Aor51HI AGCGCT 1 cut(s) 340
AoxI GGCC 7 cut(s) 3, 57, 129, 198, 202, 280, 297
ApeKI GCWGC 6 cut(s) 85, 479, 551, 617, 626, 629
Asp700I GAANNNNTTC 1 cut(s) 419
Asp718I GGTACC 1 cut(s) 654
AspLEI GCGC 4 cut(s) 90, 145, 169, 341
AspS9I GGNCC 5 cut(s) 58, 67, 154, 313, 402
AsuC2I CCSGG 1 cut(s) 652
AsuII TTCGAA 1 cut(s) 423
AvaI CYCGRG 1 cut(s) 62
AvaII GGWCC 4 cut(s) 67, 154, 313, 402
BanI GGYRCC 1 cut(s) 654
BanII GRGCYC 2 cut(s) 276, 357
BbrPI CACGTG 1 cut(s) 139
BbsI GAAGAC 1 cut(s) 377
Bbv12I GWGCWC 2 cut(s) 276, 357
BbvI GCAGC 6 cut(s) 97, 466, 538, 604, 638, 641
BccI CCATC 1 cut(s) 343
BceAI ACGGC 1 cut(s) 72
BcnI CCSGG 1 cut(s) 652
BfaI CTAG 1 cut(s) 399
BfmI CTRYAG 1 cut(s) 618
BfoI RGCGCY 2 cut(s) 146, 342
BfuAI ACCTGC 1 cut(s) 635
BglI GCCNNNNNGGC 1 cut(s) 296
BisI GCNGC 8 cut(s) 86, 129, 280, 480, 552, 618, 627, 630
BlpI GCTNAGC 1 cut(s) 758
BlsI GCNGC 8 cut(s) 87, 130, 281, 481, 553, 619, 628, 631
Bme1390I CCNGG 1 cut(s) 652
Bme18I GGWCC 4 cut(s) 67, 154, 313, 402
BmeT110I CYCGRG 1 cut(s) 62
BmgT120I GGNCC 5 cut(s) 58, 67, 154, 313, 402
BmiI GGNNCC 2 cut(s) 649, 656
BmrFI CCNGG 1 cut(s) 652
BmsI GCATC 3 cut(s) 31, 532, 733
BpiI GAAGAC 1 cut(s) 377
BpmI CTGGAG 1 cut(s) 65
Bpu1102I GCTNAGC 1 cut(s) 758
Bpu14I TTCGAA 1 cut(s) 423
BpuEI CTTGAG 2 cut(s) 131, 355
BpuMI CCSGG 1 cut(s) 652
BsaAI YACGTR 1 cut(s) 139
BsaHI GRCGYC 1 cut(s) 288
BsaJI CCNNGG 3 cut(s) 17, 277, 379
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 193, 644
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 4 cut(s) 97, 438, 510, 638
Bse3DI GCAATG 1 cut(s) 679
BseDI CCNNGG 3 cut(s) 17, 277, 379
BseGI GGATG 1 cut(s) 354
BseLI CCNNNNNNNGG 3 cut(s) 163, 193, 644
BseMI GCAATG 1 cut(s) 679
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 4 cut(s) 97, 438, 510, 638
BseRI GAGGAG 1 cut(s) 95
BseXI GCAGC 6 cut(s) 97, 466, 538, 604, 638, 641
Bsh1236I CGCG 6 cut(s) 72, 90, 128, 227, 279, 359
Bsh1285I CGRYCG 1 cut(s) 363
BshFI GGCC 7 cut(s) 5, 59, 131, 200, 204, 282, 299
BshNI GGYRCC 1 cut(s) 654
BsiEI CGRYCG 1 cut(s) 363
BsiHKAI GWGCWC 2 cut(s) 276, 357
BsiHKCI CYCGRG 1 cut(s) 62
BsiSI CCGG 3 cut(s) 6, 201, 651
BslFI GGGAC 1 cut(s) 85
BslI CCNNNNNNNGG 3 cut(s) 163, 193, 644
BsmFI GGGAC 1 cut(s) 85
BsnI GGCC 7 cut(s) 5, 59, 131, 200, 204, 282, 299
BsoBI CYCGRG 1 cut(s) 62
Bsp119I TTCGAA 1 cut(s) 423
Bsp1286I GDGCHC 2 cut(s) 276, 357
Bsp143I GATC 3 cut(s) 76, 93, 375
Bsp1720I GCTNAGC 1 cut(s) 758
Bsp68I TCGCGA 1 cut(s) 359
BspANI GGCC 7 cut(s) 5, 59, 131, 200, 204, 282, 299
BspCNI CTCAG 1 cut(s) 297
BspFNI CGCG 6 cut(s) 72, 90, 128, 227, 279, 359
BspLI GGNNCC 2 cut(s) 649, 656
BspMAI CTGCAG 1 cut(s) 622
BspMI ACCTGC 1 cut(s) 635
BspPI GGATC 2 cut(s) 88, 370
BspT104I TTCGAA 1 cut(s) 423
BspT107I GGYRCC 1 cut(s) 654
BsrDI GCAATG 1 cut(s) 679
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 4 cut(s) 97, 438, 510, 638
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 3 cut(s) 17, 277, 379
BssMI GATC 3 cut(s) 76, 93, 375
BssNAI GTATAC 1 cut(s) 244
BssNI GRCGYC 1 cut(s) 288
BssT1I CCWWGG 1 cut(s) 379
Bst1107I GTATAC 1 cut(s) 244
Bst6I CTCTTC 2 cut(s) 4, 599
BstACI GRCGYC 1 cut(s) 288
BstAPI GCANNNNNTGC 1 cut(s) 617
BstBAI YACGTR 1 cut(s) 139
BstBI TTCGAA 1 cut(s) 423
BstC8I GCNNGC 2 cut(s) 202, 357
BstDEI CTNAG 2 cut(s) 305, 758
BstDSI CCRYGG 1 cut(s) 277
BstF5I GGATG 1 cut(s) 354
BstFNI CGCG 6 cut(s) 72, 90, 128, 227, 279, 359
BstH2I RGCGCY 2 cut(s) 146, 342
BstHHI GCGC 4 cut(s) 90, 145, 169, 341
BstKTI GATC 3 cut(s) 79, 96, 378
BstMBI GATC 3 cut(s) 76, 93, 375
BstMCI CGRYCG 1 cut(s) 363
BstMWI GCNNNNNNNGC 6 cut(s) 180, 296, 557, 617, 623, 626
BstSCI CCNGG 1 cut(s) 650
BstSFI CTRYAG 1 cut(s) 618
BstUI CGCG 6 cut(s) 72, 90, 128, 227, 279, 359
BstV1I GCAGC 6 cut(s) 97, 466, 538, 604, 638, 641
BstV2I GAAGAC 1 cut(s) 377
BstX2I RGATCY 1 cut(s) 93
BstXI CCANNNNNNTGG 1 cut(s) 139
BstYI RGATCY 1 cut(s) 93
BstZ17I GTATAC 1 cut(s) 244
BsuRI GGCC 7 cut(s) 5, 59, 131, 200, 204, 282, 299
BtgI CCRYGG 1 cut(s) 277
BtsCI GGATG 1 cut(s) 354
BtsI GCAGTG 1 cut(s) 733
BtsIMutI CAGTG 1 cut(s) 733
BtuMI TCGCGA 1 cut(s) 359
BveI ACCTGC 1 cut(s) 635
Cac8I GCNNGC 2 cut(s) 202, 357
CaiI CAGNNNCTG 1 cut(s) 157
CfoI GCGC 4 cut(s) 90, 145, 169, 341
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 5 cut(s) 58, 67, 154, 313, 402
Cfr42I CCGCGG 1 cut(s) 280
CseI GACGC 2 cut(s) 61, 296
Csp6I GTAC 1 cut(s) 655
CviAII CATG 2 cut(s) 292, 581
CviQI GTAC 1 cut(s) 655
DdeI CTNAG 2 cut(s) 305, 758
DpnI GATC 3 cut(s) 78, 95, 377
DpnII GATC 3 cut(s) 76, 93, 375
EaeI YGGCCR 4 cut(s) 3, 129, 198, 202
Eam1104I CTCTTC 2 cut(s) 4, 599
EarI CTCTTC 2 cut(s) 4, 599
Ecl136II GAGCTC 2 cut(s) 274, 355
Eco130I CCWWGG 1 cut(s) 379
Eco24I GRGCYC 2 cut(s) 276, 357
Eco47I GGWCC 4 cut(s) 67, 154, 313, 402
Eco47III AGCGCT 1 cut(s) 340
Eco53kI GAGCTC 2 cut(s) 274, 355
Eco57I CTGAAG 1 cut(s) 209
Eco72I CACGTG 1 cut(s) 139
Eco88I CYCGRG 1 cut(s) 62
EcoICRI GAGCTC 2 cut(s) 274, 355
EcoO109I RGGNCCY 1 cut(s) 154
EcoT14I CCWWGG 1 cut(s) 379
EcoT22I ATGCAT 1 cut(s) 703
EcoT38I GRGCYC 2 cut(s) 276, 357
ErhI CCWWGG 1 cut(s) 379
FaeI CATG 2 cut(s) 295, 584
FaqI GGGAC 1 cut(s) 85
FatI CATG 2 cut(s) 291, 580
FauI CCCGC 1 cut(s) 228
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 8 cut(s) 86, 129, 280, 480, 552, 618, 627, 630
FokI GGATG 1 cut(s) 361
FriOI GRGCYC 2 cut(s) 276, 357
FseI GGCCGGCC 1 cut(s) 204
Fsp4HI GCNGC 8 cut(s) 86, 129, 280, 480, 552, 618, 627, 630
FspBI CTAG 1 cut(s) 399
GlaI GCGC 4 cut(s) 89, 144, 168, 340
GluI GCNGC 8 cut(s) 86, 129, 280, 480, 552, 618, 627, 630
GsuI CTGGAG 1 cut(s) 65
HaeII RGCGCY 2 cut(s) 146, 342
HaeIII GGCC 7 cut(s) 5, 59, 131, 200, 204, 282, 299
HapII CCGG 3 cut(s) 6, 201, 651
HgaI GACGC 2 cut(s) 61, 296
HhaI GCGC 4 cut(s) 90, 145, 169, 341
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 2 cut(s) 295, 584
Hin6I GCGC 4 cut(s) 88, 143, 167, 339
HinP1I GCGC 4 cut(s) 88, 143, 167, 339
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HindIII AAGCTT 1 cut(s) 575
HinfI GANTC 1 cut(s) 410
HpaII CCGG 3 cut(s) 6, 201, 651
Hpy166II GTNNAC 2 cut(s) 49, 244
Hpy188I TCNGA 2 cut(s) 217, 406
Hpy188III TCNNGA 4 cut(s) 316, 358, 563, 728
Hpy8I GTNNAC 2 cut(s) 49, 244
Hpy99I CGWCG 4 cut(s) 58, 77, 272, 410
HpyAV CCTTC 4 cut(s) 184, 289, 448, 468
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 5 cut(s) 523, 620, 644, 701, 724
HpyF10VI GCNNNNNNNGC 6 cut(s) 180, 296, 557, 617, 623, 626
HpyF3I CTNAG 2 cut(s) 305, 758
HpySE526I ACGT 1 cut(s) 138
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 2 cut(s) 295, 584
HspAI GCGC 4 cut(s) 88, 143, 167, 339
KpnI GGTACC 1 cut(s) 658
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
KspI CCGCGG 1 cut(s) 280
Kzo9I GATC 3 cut(s) 76, 93, 375
LmnI GCTCC 2 cut(s) 279, 352
Lsp1109I GCAGC 6 cut(s) 97, 466, 538, 604, 638, 641
LweI GCATC 3 cut(s) 31, 532, 733
MaeI CTAG 1 cut(s) 399
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 1 cut(s) 512
MalI GATC 3 cut(s) 78, 95, 377
MboI GATC 3 cut(s) 76, 93, 375
MboII GAAGA 4 cut(s) 21, 382, 508, 616
MflI RGATCY 1 cut(s) 93
MhlI GDGCHC 2 cut(s) 276, 357
MluCI AATT 3 cut(s) 419, 441, 742
MlyI GAGTC 1 cut(s) 419
MmeI TCCRAC 1 cut(s) 429
Mph1103I ATGCAT 1 cut(s) 703
MroNI GCCGGC 1 cut(s) 200
MroXI GAANNNNTTC 1 cut(s) 419
MseI TTAA 2 cut(s) 444, 778
MslI CAYNNNNRTG 1 cut(s) 137
MspA1I CMGCKG 1 cut(s) 279
MspI CCGG 3 cut(s) 6, 201, 651
MspR9I CCNGG 1 cut(s) 652
MvnI CGCG 6 cut(s) 72, 90, 128, 227, 279, 359
MwoI GCNNNNNNNGC 6 cut(s) 180, 296, 557, 617, 623, 626
NaeI GCCGGC 1 cut(s) 202
NciI CCSGG 1 cut(s) 652
NdeII GATC 3 cut(s) 76, 93, 375
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 2 cut(s) 295, 584
NlaIV GGNNCC 2 cut(s) 649, 656
NmeAIII GCCGAG 1 cut(s) 325
NruI TCGCGA 1 cut(s) 359
NsiI ATGCAT 1 cut(s) 703
NspV TTCGAA 1 cut(s) 423
OliI CACNNNNGTG 1 cut(s) 137
PaeR7I CTCGAG 1 cut(s) 62
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PdiI GCCGGC 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 419
PflFI GACNNNGTC 2 cut(s) 71, 400
PkrI GCNGC 8 cut(s) 87, 130, 281, 481, 553, 619, 628, 631
PleI GAGTC 1 cut(s) 418
PmaCI CACGTG 1 cut(s) 139
PmlI CACGTG 1 cut(s) 139
PpsI GAGTC 1 cut(s) 418
Ppu21I YACGTR 1 cut(s) 139
PpuMI RGGWCCY 1 cut(s) 154
Psp124BI GAGCTC 2 cut(s) 276, 357
Psp5II RGGWCCY 1 cut(s) 154
PspCI CACGTG 1 cut(s) 139
PspN4I GGNNCC 2 cut(s) 649, 656
PspPI GGNCC 5 cut(s) 58, 67, 154, 313, 402
PspPPI RGGWCCY 1 cut(s) 154
PspXI VCTCGAGB 1 cut(s) 62
PstI CTGCAG 1 cut(s) 622
PstNI CAGNNNCTG 1 cut(s) 157
PsuI RGATCY 1 cut(s) 93
PsyI GACNNNGTC 2 cut(s) 71, 400
RigI GGCCGGCC 1 cut(s) 204
RruI TCGCGA 1 cut(s) 359
RsaI GTAC 1 cut(s) 656
RsaNI GTAC 1 cut(s) 655
RseI CAYNNNNRTG 1 cut(s) 137
SacI GAGCTC 2 cut(s) 276, 357
SacII CCGCGG 1 cut(s) 280
SaqAI TTAA 2 cut(s) 444, 778
SatI GCNGC 8 cut(s) 86, 129, 280, 480, 552, 618, 627, 630
Sau3AI GATC 3 cut(s) 76, 93, 375
Sau96I GGNCC 5 cut(s) 58, 67, 154, 313, 402
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 1 cut(s) 652
SduI GDGCHC 2 cut(s) 276, 357
SfaNI GCATC 3 cut(s) 31, 532, 733
SfcI CTRYAG 1 cut(s) 618
Sfr274I CTCGAG 1 cut(s) 62
Sfr303I CCGCGG 1 cut(s) 280
SfuI TTCGAA 1 cut(s) 423
SgrBI CCGCGG 1 cut(s) 280
SinI GGWCC 4 cut(s) 67, 154, 313, 402
SlaI CTCGAG 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 3 cut(s) 62, 146, 334
SmoI CTYRAG 3 cut(s) 62, 146, 334
Sse9I AATT 3 cut(s) 419, 441, 742
SspMI CTAG 1 cut(s) 399
SstI GAGCTC 2 cut(s) 276, 357
StyD4I CCNGG 1 cut(s) 650
StyI CCWWGG 1 cut(s) 379
TaiI ACGT 1 cut(s) 141
TaqI TCGA 6 cut(s) 63, 75, 327, 413, 423, 564
TaqII GACCGA 1 cut(s) 377
TasI AATT 3 cut(s) 419, 441, 742
TauI GCSGC 2 cut(s) 131, 282
Tru1I TTAA 2 cut(s) 444, 778
Tru9I TTAA 2 cut(s) 444, 778
TscAI CASTG 1 cut(s) 740
TseI GCWGC 6 cut(s) 85, 479, 551, 617, 626, 629
TspDTI ATGAA 2 cut(s) 308, 569
TspGWI ACGGA 1 cut(s) 705
TspRI CASTG 1 cut(s) 740
Tth111I GACNNNGTC 2 cut(s) 71, 400
VpaK11BI GGWCC 4 cut(s) 67, 154, 313, 402
XhoI CTCGAG 1 cut(s) 62
XmiI GTMKAC 1 cut(s) 243
XmnI GAANNNNTTC 1 cut(s) 419
XspI CTAG 1 cut(s) 399
Zsp2I ATGCAT 1 cut(s) 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.