RchiOBHm_Chr5g0071291

Protein FLC EXPRESSOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000946
Physical Location & Seq
Reverse (-)
77193949 .. 77195969
2021 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34639

Sequence Viewer

Length: 816 bp
ATGGCCGGAAGAGAGCCTCGGCATCTCCACCGCCTTCCCCAAATCGTCAACACGACGGCCCTCGAGGACCGCATCGATCTCCAGCAACGCGAGATCCAGTCCCTCCTCGGGGAGAATCAACGCCTCGCGGCCACCCACGTGGCGCTCAAGCAGGACCTAACCGCCGCTCAGAGCGACCTCCGCAACCTGAAGGCCGTCGCCGGCCAAATCAAATCCGAGAGGAACGCCGAAGTCCGCGAGGTCTACGACAGGTCGTTGAAGCTGGACGACGAGCTCCGCGACCTGGACGCCATGAACGCCGAGTTGACTCTGGTCCGGAACGACATCGAGGACTTGAGCACGTCGCGGATGGAGCTCGCGACCGAGTTGAAGACGATCCAAGGAGAGATTGAGAGGGCTCGCTCCGACGAGTCGAAGCAAATCGAAGCCATTAGAGACGAAATTGAGACCTTGAAGCTGGAGATTGAGAAAGGAAGTACTGCGGTTGAGATTGAGAAGAAAACGCGAGCTAGTAACCTTGAGCATCGTCAAGGTATGGAGAACTATATGGCCAAGTTGGCTCTTGAAATCGAAAAGCTTCATGGGGAGTTGGCAAACGCAGAGAAGAGAGCAAGGGCTGCAGTAGCAGCTGCAAATCCAGGTTCTGGGTACCCTATGGCTTATGGCAATGCCGAAATGCTATATGGAGGAATTGCATACCCTGATCCCTATGCCATGCATCAGGGTCAGGGCAGTGCTGATGGCACCCCTCAATATGGTTCCGCTCAAATGCCACATAGTTCCTATGATATTCAACAAACACATGTACATAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

271

Amino Acids

30.28

Weight (kDa)

5.38

Isoelectric Point (pI)

50.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 62
Acc65I GGTACC 1 cut(s) 648
AccB1I GGYRCC 2 cut(s) 648, 743
AccB7I CCANNNNNTGG 1 cut(s) 644
AccBSI CCGCTC 2 cut(s) 167, 764
AccI GTMKAC 1 cut(s) 243
AccII CGCG 7 cut(s) 90, 128, 237, 279, 346, 359, 505
AccIII TCCGGA 1 cut(s) 315
AclWI GGATC 3 cut(s) 88, 370, 698
AcoI YGGCCR 4 cut(s) 3, 129, 202, 549
AcuI CTGAAG 1 cut(s) 209
AcvI CACGTG 1 cut(s) 139
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 3 cut(s) 478, 650, 807
AfiI CCNNNNNNNGG 5 cut(s) 108, 109, 283, 644, 755
AflIII ACRYGT 1 cut(s) 802
AgsI TTSAA 5 cut(s) 259, 370, 454, 566, 794
AjiI CACGTC 1 cut(s) 342
AjnI CCWGG 2 cut(s) 282, 637
AleI CACNNNNGTG 1 cut(s) 137
AluBI AGCT 7 cut(s) 262, 274, 355, 457, 509, 577, 629
AluI AGCT 7 cut(s) 262, 274, 355, 457, 509, 577, 629
Alw21I GWGCWC 3 cut(s) 276, 341, 357
Alw26I GTCTC 2 cut(s) 429, 440
AlwI GGATC 3 cut(s) 88, 370, 698
AlwNI CAGNNNCTG 1 cut(s) 644
Ama87I CYCGRG 2 cut(s) 62, 107
Aor13HI TCCGGA 1 cut(s) 315
AoxI GGCC 6 cut(s) 3, 57, 129, 192, 202, 549
ApeKI GCWGC 3 cut(s) 617, 626, 629
Asp700I GAANNNNTTC 1 cut(s) 576
Asp718I GGTACC 1 cut(s) 648
AspLEI GCGC 1 cut(s) 145
AspS9I GGNCC 4 cut(s) 58, 67, 154, 313
AvaI CYCGRG 2 cut(s) 62, 107
AvaII GGWCC 3 cut(s) 67, 154, 313
BalI TGGCCA 1 cut(s) 551
BanI GGYRCC 2 cut(s) 648, 743
BanII GRGCYC 3 cut(s) 276, 357, 400
BbrPI CACGTG 1 cut(s) 139
BbsI GAAGAC 1 cut(s) 377
Bbv12I GWGCWC 3 cut(s) 276, 341, 357
BbvI GCAGC 3 cut(s) 604, 616, 638
BccI CCATC 2 cut(s) 343, 734
BceAI ACGGC 2 cut(s) 72, 179
BciT130I CCWGG 2 cut(s) 284, 639
BcoDI GTCTC 2 cut(s) 429, 440
BfaI CTAG 1 cut(s) 510
BfmI CTRYAG 1 cut(s) 618
BfoI RGCGCY 1 cut(s) 146
BglI GCCNNNNNGGC 1 cut(s) 557
BisI GCNGC 5 cut(s) 129, 165, 618, 627, 630
BlsI GCNGC 5 cut(s) 130, 166, 619, 628, 631
BmcAI AGTACT 1 cut(s) 478
Bme1390I CCNGG 2 cut(s) 284, 639
Bme18I GGWCC 3 cut(s) 67, 154, 313
BmeT110I CYCGRG 2 cut(s) 62, 107
BmgBI CACGTC 1 cut(s) 342
BmgT120I GGNCC 4 cut(s) 58, 67, 154, 313
BmiI GGNNCC 3 cut(s) 650, 745, 760
BmrFI CCNGG 2 cut(s) 284, 639
BmsI GCATC 4 cut(s) 31, 81, 532, 727
BoxI GACNNNNGTC 1 cut(s) 311
BpiI GAAGAC 1 cut(s) 377
BpmI CTGGAG 2 cut(s) 65, 479
BpuEI CTTGAG 3 cut(s) 131, 355, 539
Bsa29I ATCGAT 1 cut(s) 75
BsaAI YACGTR 1 cut(s) 139
BsaHI GRCGYC 1 cut(s) 288
BsaI GGTCTC 1 cut(s) 440
BsaJI CCNNGG 3 cut(s) 17, 106, 379
BsaWI WCCGGW 1 cut(s) 315
Bsc4I CCNNNNNNNGG 5 cut(s) 108, 109, 283, 644, 755
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 97
Bse3DI GCAATG 1 cut(s) 673
BseAI TCCGGA 1 cut(s) 315
BseBI CCWGG 2 cut(s) 284, 639
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 3 cut(s) 17, 106, 379
BseGI GGATG 1 cut(s) 354
BseLI CCNNNNNNNGG 5 cut(s) 108, 109, 283, 644, 755
BseMI GCAATG 1 cut(s) 673
BseMII CTCAG 1 cut(s) 182
BseNI ACTGG 1 cut(s) 97
BseRI GAGGAG 1 cut(s) 95
BseXI GCAGC 3 cut(s) 604, 616, 638
Bsh1236I CGCG 7 cut(s) 90, 128, 237, 279, 346, 359, 505
Bsh1285I CGRYCG 1 cut(s) 363
BshFI GGCC 6 cut(s) 5, 59, 131, 194, 204, 551
BshNI GGYRCC 2 cut(s) 648, 743
BshVI ATCGAT 1 cut(s) 75
BsiEI CGRYCG 1 cut(s) 363
BsiHKAI GWGCWC 3 cut(s) 276, 341, 357
BsiHKCI CYCGRG 2 cut(s) 62, 107
BsiSI CCGG 3 cut(s) 6, 201, 316
BslFI GGGAC 1 cut(s) 85
BslI CCNNNNNNNGG 5 cut(s) 108, 109, 283, 644, 755
BsmAI GTCTC 2 cut(s) 429, 440
BsmBI CGTCTC 1 cut(s) 429
BsmFI GGGAC 1 cut(s) 85
BsnI GGCC 6 cut(s) 5, 59, 131, 194, 204, 551
Bso31I GGTCTC 1 cut(s) 440
BsoBI CYCGRG 2 cut(s) 62, 107
Bsp1286I GDGCHC 4 cut(s) 276, 341, 357, 400
Bsp13I TCCGGA 1 cut(s) 315
Bsp1407I TGTACA 1 cut(s) 805
Bsp143I GATC 4 cut(s) 76, 93, 375, 703
Bsp68I TCGCGA 1 cut(s) 359
BspANI GGCC 6 cut(s) 5, 59, 131, 194, 204, 551
BspCNI CTCAG 1 cut(s) 181
BspDI ATCGAT 1 cut(s) 75
BspEI TCCGGA 1 cut(s) 315
BspFNI CGCG 7 cut(s) 90, 128, 237, 279, 346, 359, 505
BspLI GGNNCC 3 cut(s) 650, 745, 760
BspMAI CTGCAG 1 cut(s) 622
BspPI GGATC 3 cut(s) 88, 370, 698
BspT107I GGYRCC 2 cut(s) 648, 743
BspTNI GGTCTC 1 cut(s) 440
BsrBI CCGCTC 2 cut(s) 167, 764
BsrDI GCAATG 1 cut(s) 673
BsrFI RCCGGY 1 cut(s) 200
BsrGI TGTACA 1 cut(s) 805
BsrI ACTGG 1 cut(s) 97
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 3 cut(s) 17, 106, 379
BssMI GATC 4 cut(s) 76, 93, 375, 703
BssNI GRCGYC 1 cut(s) 288
BssT1I CCWWGG 1 cut(s) 379
Bst2UI CCWGG 2 cut(s) 284, 639
Bst6I CTCTTC 2 cut(s) 4, 599
BstACI GRCGYC 1 cut(s) 288
BstAPI GCANNNNNTGC 1 cut(s) 617
BstAUI TGTACA 1 cut(s) 805
BstBAI YACGTR 1 cut(s) 139
BstC8I GCNNGC 4 cut(s) 202, 357, 400, 507
BstDEI CTNAG 1 cut(s) 168
BstF5I GGATG 1 cut(s) 354
BstFNI CGCG 7 cut(s) 90, 128, 237, 279, 346, 359, 505
BstH2I RGCGCY 1 cut(s) 146
BstHHI GCGC 1 cut(s) 145
BstKTI GATC 4 cut(s) 79, 96, 378, 706
BstMAI GTCTC 2 cut(s) 429, 440
BstMBI GATC 4 cut(s) 76, 93, 375, 703
BstMCI CGRYCG 1 cut(s) 363
BstMWI GCNNNNNNNGC 7 cut(s) 180, 296, 352, 557, 617, 623, 626
BstNI CCWGG 2 cut(s) 284, 639
BstNSI RCATGY 1 cut(s) 806
BstPAI GACNNNNGTC 1 cut(s) 311
BstSCI CCNGG 2 cut(s) 282, 637
BstSFI CTRYAG 1 cut(s) 618
BstUI CGCG 7 cut(s) 90, 128, 237, 279, 346, 359, 505
BstV1I GCAGC 3 cut(s) 604, 616, 638
BstV2I GAAGAC 1 cut(s) 377
BstX2I RGATCY 1 cut(s) 93
BstXI CCANNNNNNTGG 1 cut(s) 139
BstYI RGATCY 1 cut(s) 93
Bsu15I ATCGAT 1 cut(s) 75
BsuRI GGCC 6 cut(s) 5, 59, 131, 194, 204, 551
BsuTUI ATCGAT 1 cut(s) 75
BtrI CACGTC 1 cut(s) 342
BtsCI GGATG 1 cut(s) 354
BtsI GCAGTG 1 cut(s) 739
BtsIMutI CAGTG 1 cut(s) 739
BtuMI TCGCGA 1 cut(s) 359
Cac8I GCNNGC 4 cut(s) 202, 357, 400, 507
CaiI CAGNNNCTG 1 cut(s) 644
CfoI GCGC 1 cut(s) 145
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 4 cut(s) 58, 67, 154, 313
ClaI ATCGAT 1 cut(s) 75
CseI GACGC 1 cut(s) 296
Csp6I GTAC 3 cut(s) 477, 649, 806
CviAII CATG 4 cut(s) 292, 581, 715, 803
CviQI GTAC 3 cut(s) 477, 649, 806
DdeI CTNAG 1 cut(s) 168
DpnI GATC 4 cut(s) 78, 95, 377, 705
DpnII GATC 4 cut(s) 76, 93, 375, 703
EaeI YGGCCR 4 cut(s) 3, 129, 202, 549
Eam1104I CTCTTC 2 cut(s) 4, 599
EarI CTCTTC 2 cut(s) 4, 599
Ecl136II GAGCTC 2 cut(s) 274, 355
Eco130I CCWWGG 1 cut(s) 379
Eco24I GRGCYC 3 cut(s) 276, 357, 400
Eco31I GGTCTC 1 cut(s) 440
Eco47I GGWCC 3 cut(s) 67, 154, 313
Eco53kI GAGCTC 2 cut(s) 274, 355
Eco57I CTGAAG 1 cut(s) 209
Eco72I CACGTG 1 cut(s) 139
Eco88I CYCGRG 2 cut(s) 62, 107
EcoICRI GAGCTC 2 cut(s) 274, 355
EcoO109I RGGNCCY 1 cut(s) 154
EcoRII CCWGG 2 cut(s) 282, 637
EcoT14I CCWWGG 1 cut(s) 379
EcoT22I ATGCAT 1 cut(s) 720
EcoT38I GRGCYC 3 cut(s) 276, 357, 400
ErhI CCWWGG 1 cut(s) 379
Esp3I CGTCTC 1 cut(s) 429
FaeI CATG 4 cut(s) 295, 584, 718, 806
FaqI GGGAC 1 cut(s) 85
FatI CATG 4 cut(s) 291, 580, 714, 802
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 5 cut(s) 129, 165, 618, 627, 630
FokI GGATG 1 cut(s) 361
FriOI GRGCYC 3 cut(s) 276, 357, 400
Fsp4HI GCNGC 5 cut(s) 129, 165, 618, 627, 630
FspBI CTAG 1 cut(s) 510
GlaI GCGC 1 cut(s) 144
GluI GCNGC 5 cut(s) 129, 165, 618, 627, 630
GsuI CTGGAG 2 cut(s) 65, 479
HaeII RGCGCY 1 cut(s) 146
HaeIII GGCC 6 cut(s) 5, 59, 131, 194, 204, 551
HapII CCGG 3 cut(s) 6, 201, 316
HgaI GACGC 1 cut(s) 296
HhaI GCGC 1 cut(s) 145
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 4 cut(s) 295, 584, 718, 806
Hin6I GCGC 1 cut(s) 143
HinP1I GCGC 1 cut(s) 143
HincII GTYRAC 2 cut(s) 49, 306
HindII GTYRAC 2 cut(s) 49, 306
HindIII AAGCTT 1 cut(s) 575
HinfI GANTC 3 cut(s) 115, 307, 410
HpaII CCGG 3 cut(s) 6, 201, 316
Hpy166II GTNNAC 3 cut(s) 49, 244, 306
Hpy188I TCNGA 3 cut(s) 171, 217, 406
Hpy188III TCNNGA 3 cut(s) 316, 358, 563
Hpy8I GTNNAC 3 cut(s) 49, 244, 306
Hpy99I CGWCG 5 cut(s) 58, 200, 272, 346, 410
HpyAV CCTTC 2 cut(s) 44, 184
HpyCH4IV ACGT 2 cut(s) 138, 341
HpyCH4V TGCA 4 cut(s) 620, 632, 695, 718
HpyF10VI GCNNNNNNNGC 7 cut(s) 180, 296, 352, 557, 617, 623, 626
HpyF3I CTNAG 1 cut(s) 168
HpySE526I ACGT 2 cut(s) 138, 341
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 4 cut(s) 295, 584, 718, 806
HspAI GCGC 1 cut(s) 143
Kpn2I TCCGGA 1 cut(s) 315
KpnI GGTACC 1 cut(s) 652
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Kzo9I GATC 4 cut(s) 76, 93, 375, 703
LmnI GCTCC 3 cut(s) 279, 352, 407
Lsp1109I GCAGC 3 cut(s) 604, 616, 638
LweI GCATC 4 cut(s) 31, 81, 532, 727
MaeI CTAG 1 cut(s) 510
MaeII ACGT 2 cut(s) 138, 341
MaeIII GTNAC 1 cut(s) 512
MalI GATC 4 cut(s) 78, 95, 377, 705
MbiI CCGCTC 2 cut(s) 167, 764
MboI GATC 4 cut(s) 76, 93, 375, 703
MboII GAAGA 4 cut(s) 21, 382, 508, 616
MflI RGATCY 1 cut(s) 93
MhlI GDGCHC 4 cut(s) 276, 341, 357, 400
MlsI TGGCCA 1 cut(s) 551
MluCI AATT 2 cut(s) 441, 690
MluNI TGGCCA 1 cut(s) 551
MlyI GAGTC 2 cut(s) 301, 419
MmeI TCCRAC 1 cut(s) 429
Mox20I TGGCCA 1 cut(s) 551
Mph1103I ATGCAT 1 cut(s) 720
MroI TCCGGA 1 cut(s) 315
MroNI GCCGGC 1 cut(s) 200
MroXI GAANNNNTTC 1 cut(s) 576
MscI TGGCCA 1 cut(s) 551
MslI CAYNNNNRTG 1 cut(s) 137
Msp20I TGGCCA 1 cut(s) 551
MspA1I CMGCKG 1 cut(s) 629
MspI CCGG 3 cut(s) 6, 201, 316
MspR9I CCNGG 2 cut(s) 284, 639
MvaI CCWGG 2 cut(s) 284, 639
MvnI CGCG 7 cut(s) 90, 128, 237, 279, 346, 359, 505
MwoI GCNNNNNNNGC 7 cut(s) 180, 296, 352, 557, 617, 623, 626
NaeI GCCGGC 1 cut(s) 202
NdeII GATC 4 cut(s) 76, 93, 375, 703
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 4 cut(s) 295, 584, 718, 806
NlaIV GGNNCC 3 cut(s) 650, 745, 760
NmeAIII GCCGAG 1 cut(s) 325
NruI TCGCGA 1 cut(s) 359
NsiI ATGCAT 1 cut(s) 720
NspI RCATGY 1 cut(s) 806
OliI CACNNNNGTG 1 cut(s) 137
PaeR7I CTCGAG 1 cut(s) 62
PciI ACATGT 1 cut(s) 802
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PdiI GCCGGC 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 576
PfeI GAWTC 1 cut(s) 115
PflMI CCANNNNNTGG 1 cut(s) 644
PkrI GCNGC 5 cut(s) 130, 166, 619, 628, 631
PleI GAGTC 2 cut(s) 301, 418
PmaCI CACGTG 1 cut(s) 139
PmlI CACGTG 1 cut(s) 139
PpsI GAGTC 2 cut(s) 301, 418
Ppu21I YACGTR 1 cut(s) 139
PpuMI RGGWCCY 1 cut(s) 154
PscI ACATGT 1 cut(s) 802
PshAI GACNNNNGTC 1 cut(s) 311
Psp124BI GAGCTC 2 cut(s) 276, 357
Psp5II RGGWCCY 1 cut(s) 154
Psp6I CCWGG 2 cut(s) 282, 637
PspCI CACGTG 1 cut(s) 139
PspGI CCWGG 2 cut(s) 282, 637
PspN4I GGNNCC 3 cut(s) 650, 745, 760
PspPI GGNCC 4 cut(s) 58, 67, 154, 313
PspPPI RGGWCCY 1 cut(s) 154
PspXI VCTCGAGB 1 cut(s) 62
PstI CTGCAG 1 cut(s) 622
PstNI CAGNNNCTG 1 cut(s) 644
PsuI RGATCY 1 cut(s) 93
PvuII CAGCTG 1 cut(s) 629
RruI TCGCGA 1 cut(s) 359
RsaI GTAC 3 cut(s) 478, 650, 807
RsaNI GTAC 3 cut(s) 477, 649, 806
RseI CAYNNNNRTG 1 cut(s) 137
SacI GAGCTC 2 cut(s) 276, 357
SatI GCNGC 5 cut(s) 129, 165, 618, 627, 630
Sau3AI GATC 4 cut(s) 76, 93, 375, 703
Sau96I GGNCC 4 cut(s) 58, 67, 154, 313
ScaI AGTACT 1 cut(s) 478
SchI GAGTC 2 cut(s) 301, 419
ScrFI CCNGG 2 cut(s) 284, 639
SduI GDGCHC 4 cut(s) 276, 341, 357, 400
SfaNI GCATC 4 cut(s) 31, 81, 532, 727
SfcI CTRYAG 1 cut(s) 618
Sfr274I CTCGAG 1 cut(s) 62
SinI GGWCC 3 cut(s) 67, 154, 313
SlaI CTCGAG 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 4 cut(s) 62, 146, 334, 518
SmoI CTYRAG 4 cut(s) 62, 146, 334, 518
Sse9I AATT 2 cut(s) 441, 690
SspMI CTAG 1 cut(s) 510
SstI GAGCTC 2 cut(s) 276, 357
StyD4I CCNGG 2 cut(s) 282, 637
StyI CCWWGG 1 cut(s) 379
TaiI ACGT 2 cut(s) 141, 344
TaqI TCGA 6 cut(s) 63, 75, 327, 413, 423, 570
TaqII GACCGA 1 cut(s) 377
TasI AATT 2 cut(s) 441, 690
TatI WGTACW 2 cut(s) 476, 805
TauI GCSGC 2 cut(s) 131, 167
TfiI GAWTC 1 cut(s) 115
TscAI CASTG 1 cut(s) 739
TseI GCWGC 3 cut(s) 617, 626, 629
TspDTI ATGAA 2 cut(s) 308, 569
TspRI CASTG 1 cut(s) 739
Van91I CCANNNNNTGG 1 cut(s) 644
VpaK11BI GGWCC 3 cut(s) 67, 154, 313
XceI RCATGY 1 cut(s) 806
XhoI CTCGAG 1 cut(s) 62
XmiI GTMKAC 1 cut(s) 243
XmnI GAANNNNTTC 1 cut(s) 576
XspI CTAG 1 cut(s) 510
ZrmI AGTACT 1 cut(s) 478
Zsp2I ATGCAT 1 cut(s) 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.