pycom03g04830

Protein FLC EXPRESSOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000946
Physical Location & Seq
Forward (+)
3774481 .. 3776187
1707 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g04830.4

Sequence Viewer

Length: 903 bp
ATGGCGGGACGGGAACATCCTCGACAGCTCGTCCGGCTGACGCCACTATCGGTCAATACGACGGCGCTGGAGGACCGTATCGCCCTCCAGAACCGTGAGATCCAGTCCCTCCTCGTCGACAATCAACGGCTGGCAGCCGCCCACGTTGCGCTCAAGCAGGACCTCGCCGCGGCCCAGCACGACCTCCGCCACCTATCCGTCGTCGCCGGTAAAGCCAAATCCGAGAGGGACGCCGAGGTGCGCGAGGTCTACGAGAGGGCATTGAAGCTGGACGCCGAGGTTCGCGCCATCGACTCCATGGGCGCAGACCTGGCTCGGACCCGAGCCGATATACAGGAGCTCGGCTCGTCCCGACAGGAGCTTGCGGAGGAGTTGAACACGATCGATGGCGAGCTTGCGAGGACTCAGTCCGAGGCGAACAAAGTGGCGGACATTAAAGCCGATATCGAGACCTTCAAGCAGGAGATTAAGAAAGGAAGGGATGCAATTGAGAATGAGAAGAAAACTAGAGCTAGCAACCTTGAGCACAGGCAGGGGATGGAGAAAACAATGGCGGCATTGGCTCGCGAAATGGATAAGCTTCATGGGGAGTTGGCCAATGCAGAGAAGAGAGCAAGGGCTGCAGTAGCTGCAGCAGCAAATCCAGTGCTTGATGAAATTTCTGGTTGTGTTTATGAAGGTCCTGGGTACCCTGCAGCCTATGGCAATGCTGAAATGTTATACGGGGGAAATGTGTACCCTGATCCCTATGCCGGCATGCATCAGGCCCCAGGTGCTGCTGACGCTGCCAGTCCATATGGTTCTGCACCAATGCCACATGCTTCTTATGACATGCAGCAACCGCAGGATATGAGATACAAGTCTCCAGCAGCCATTCGGACGAACAACATGGCAAAGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

301

Amino Acids

32.78

Weight (kDa)

5.83

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 687
AccB1I GGYRCC 1 cut(s) 687
AccI GTMKAC 2 cut(s) 117, 249
AccII CGCG 4 cut(s) 170, 243, 285, 567
AciI CCGC 9 cut(s) 5, 138, 168, 170, 187, 365, 428, 554, 842
AclWI GGATC 2 cut(s) 94, 737
AcoI YGGCCR 1 cut(s) 594
AcsI RAATTY 1 cut(s) 657
AcyI GRCGYC 3 cut(s) 41, 231, 273
AfaI GTAC 2 cut(s) 689, 737
AfiI CCNNNNNNNGG 2 cut(s) 169, 752
AgsI TTSAA 3 cut(s) 265, 376, 457
AhdI GACNNNNNGTC 1 cut(s) 29
AjnI CCWGG 3 cut(s) 309, 682, 769
AluBI AGCT 8 cut(s) 28, 268, 340, 361, 394, 512, 580, 629
AluI AGCT 8 cut(s) 28, 268, 340, 361, 394, 512, 580, 629
Alw21I GWGCWC 2 cut(s) 342, 528
Alw26I GTCTC 2 cut(s) 443, 867
AlwI GGATC 2 cut(s) 94, 737
AlwNI CAGNNNCTG 2 cut(s) 629, 776
Ama87I CYCGRG 1 cut(s) 321
AoxI GGCC 3 cut(s) 171, 594, 765
ApoI RAATTY 1 cut(s) 657
Asp718I GGTACC 1 cut(s) 687
AspLEI GCGC 5 cut(s) 67, 151, 243, 287, 305
AspS9I GGNCC 6 cut(s) 73, 160, 172, 318, 680, 766
AsuNHI GCTAGC 1 cut(s) 512
AvaI CYCGRG 1 cut(s) 321
AvaII GGWCC 4 cut(s) 73, 160, 318, 680
BalI TGGCCA 1 cut(s) 596
BanI GGYRCC 1 cut(s) 687
BanII GRGCYC 1 cut(s) 342
Bbv12I GWGCWC 2 cut(s) 342, 528
BccI CCATC 3 cut(s) 296, 380, 532
BceAI ACGGC 2 cut(s) 78, 143
BciT130I CCWGG 3 cut(s) 311, 684, 771
BcoDI GTCTC 2 cut(s) 443, 867
BfaI CTAG 2 cut(s) 507, 513
BfmI CTRYAG 3 cut(s) 621, 630, 693
BfoI RGCGCY 1 cut(s) 68
Bme1390I CCNGG 3 cut(s) 311, 684, 771
Bme18I GGWCC 4 cut(s) 73, 160, 318, 680
BmeRI GACNNNNNGTC 1 cut(s) 29
BmeT110I CYCGRG 1 cut(s) 321
BmgT120I GGNCC 6 cut(s) 73, 160, 172, 318, 680, 766
BmiI GGNNCC 3 cut(s) 320, 689, 768
BmrFI CCNGG 3 cut(s) 311, 684, 771
BmsI GCATC 2 cut(s) 472, 769
BmtI GCTAGC 1 cut(s) 516
BplI GAGNNNNNCTC 2 cut(s) 329, 361
BpmI CTGGAG 3 cut(s) 71, 89, 849
BpuEI CTTGAG 2 cut(s) 137, 542
Bsa29I ATCGAT 1 cut(s) 384
BsaHI GRCGYC 3 cut(s) 41, 231, 273
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 7 cut(s) 168, 234, 276, 297, 411, 683, 769
Bsc4I CCNNNNNNNGG 2 cut(s) 169, 752
Bse118I RCCGGY 2 cut(s) 206, 752
Bse1I ACTGG 3 cut(s) 103, 644, 789
Bse3DI GCAATG 1 cut(s) 712
BseBI CCWGG 3 cut(s) 311, 684, 771
BseCI ATCGAT 1 cut(s) 384
BseDI CCNNGG 7 cut(s) 168, 234, 276, 297, 411, 683, 769
BseGI GGATG 3 cut(s) 16, 487, 543
BseLI CCNNNNNNNGG 2 cut(s) 169, 752
BseMI GCAATG 1 cut(s) 712
BseMII CTCAG 1 cut(s) 419
BseNI ACTGG 3 cut(s) 103, 644, 789
BseRI GAGGAG 2 cut(s) 101, 383
BseYI CCCAGC 1 cut(s) 174
BsgI GTGCAG 1 cut(s) 789
Bsh1236I CGCG 4 cut(s) 170, 243, 285, 567
Bsh1285I CGRYCG 1 cut(s) 384
BshFI GGCC 3 cut(s) 173, 596, 767
BshNI GGYRCC 1 cut(s) 687
BshVI ATCGAT 1 cut(s) 384
BsiEI CGRYCG 1 cut(s) 384
BsiHKAI GWGCWC 2 cut(s) 342, 528
BsiHKCI CYCGRG 1 cut(s) 321
BsiSI CCGG 3 cut(s) 34, 207, 753
BslFI GGGAC 4 cut(s) 21, 91, 242, 334
BslI CCNNNNNNNGG 2 cut(s) 169, 752
BsmAI GTCTC 2 cut(s) 443, 867
BsmFI GGGAC 4 cut(s) 21, 91, 242, 334
BsnI GGCC 3 cut(s) 173, 596, 767
Bso31I GGTCTC 1 cut(s) 443
BsoBI CYCGRG 1 cut(s) 321
Bsp1286I GDGCHC 2 cut(s) 342, 528
Bsp143I GATC 3 cut(s) 99, 381, 742
Bsp19I CCATGG 1 cut(s) 297
Bsp68I TCGCGA 1 cut(s) 567
BspACI CCGC 9 cut(s) 5, 138, 168, 170, 187, 365, 428, 554, 842
BspANI GGCC 3 cut(s) 173, 596, 767
BspCNI CTCAG 1 cut(s) 418
BspDI ATCGAT 1 cut(s) 384
BspFNI CGCG 4 cut(s) 170, 243, 285, 567
BspLI GGNNCC 3 cut(s) 320, 689, 768
BspMAI CTGCAG 3 cut(s) 625, 634, 697
BspOI GCTAGC 1 cut(s) 516
BspPI GGATC 2 cut(s) 94, 737
BspT107I GGYRCC 1 cut(s) 687
BspTNI GGTCTC 1 cut(s) 443
BsrDI GCAATG 1 cut(s) 712
BsrFI RCCGGY 2 cut(s) 206, 752
BsrI ACTGG 3 cut(s) 103, 644, 789
BssAI RCCGGY 2 cut(s) 206, 752
BssECI CCNNGG 7 cut(s) 168, 234, 276, 297, 411, 683, 769
BssMI GATC 3 cut(s) 99, 381, 742
BssNI GRCGYC 3 cut(s) 41, 231, 273
BssT1I CCWWGG 1 cut(s) 297
Bst2UI CCWGG 3 cut(s) 311, 684, 771
Bst4CI ACNGT 2 cut(s) 77, 95
Bst6I CTCTTC 1 cut(s) 602
BstACI GRCGYC 3 cut(s) 41, 231, 273
BstAPI GCANNNNNTGC 2 cut(s) 620, 629
BstC8I GCNNGC 8 cut(s) 132, 363, 392, 396, 514, 565, 754, 758
BstDEI CTNAG 1 cut(s) 405
BstDSI CCRYGG 2 cut(s) 168, 297
BstF5I GGATG 3 cut(s) 16, 487, 543
BstFNI CGCG 4 cut(s) 170, 243, 285, 567
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 5 cut(s) 67, 151, 243, 287, 305
BstKTI GATC 3 cut(s) 102, 384, 745
BstMAI GTCTC 2 cut(s) 443, 867
BstMBI GATC 3 cut(s) 99, 381, 742
BstMCI CGRYCG 1 cut(s) 384
BstNI CCWGG 3 cut(s) 311, 684, 771
BstNSI RCATGY 3 cut(s) 760, 821, 835
BstSCI CCNGG 3 cut(s) 309, 682, 769
BstSFI CTRYAG 3 cut(s) 621, 630, 693
BstUI CGCG 4 cut(s) 170, 243, 285, 567
BstX2I RGATCY 1 cut(s) 99
BstYI RGATCY 1 cut(s) 99
Bsu15I ATCGAT 1 cut(s) 384
BsuRI GGCC 3 cut(s) 173, 596, 767
BsuTUI ATCGAT 1 cut(s) 384
BtgI CCRYGG 2 cut(s) 168, 297
BtsCI GGATG 3 cut(s) 16, 487, 543
BtsIMutI CAGTG 1 cut(s) 651
BtuMI TCGCGA 1 cut(s) 567
Cac8I GCNNGC 8 cut(s) 132, 363, 392, 396, 514, 565, 754, 758
CaiI CAGNNNCTG 2 cut(s) 629, 776
CfoI GCGC 5 cut(s) 67, 151, 243, 287, 305
Cfr10I RCCGGY 2 cut(s) 206, 752
Cfr13I GGNCC 6 cut(s) 73, 160, 172, 318, 680, 766
Cfr42I CCGCGG 1 cut(s) 171
ClaI ATCGAT 1 cut(s) 384
CseI GACGC 4 cut(s) 49, 239, 281, 791
Csp6I GTAC 2 cut(s) 688, 736
CviAII CATG 6 cut(s) 298, 584, 757, 818, 832, 889
CviQI GTAC 2 cut(s) 688, 736
DdeI CTNAG 1 cut(s) 405
DpnI GATC 3 cut(s) 101, 383, 744
DpnII GATC 3 cut(s) 99, 381, 742
DriI GACNNNNNGTC 1 cut(s) 29
EaeI YGGCCR 1 cut(s) 594
Eam1104I CTCTTC 1 cut(s) 602
Eam1105I GACNNNNNGTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 602
EciI GGCGGA 2 cut(s) 176, 443
Ecl136II GAGCTC 1 cut(s) 340
Eco130I CCWWGG 1 cut(s) 297
Eco24I GRGCYC 1 cut(s) 342
Eco31I GGTCTC 1 cut(s) 443
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 4 cut(s) 73, 160, 318, 680
Eco53kI GAGCTC 1 cut(s) 340
Eco88I CYCGRG 1 cut(s) 321
EcoICRI GAGCTC 1 cut(s) 340
EcoO109I RGGNCCY 3 cut(s) 160, 680, 766
EcoRII CCWGG 3 cut(s) 309, 682, 769
EcoRV GATATC 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 297
EcoT22I ATGCAT 1 cut(s) 762
EcoT38I GRGCYC 1 cut(s) 342
ErhI CCWWGG 1 cut(s) 297
FaeI CATG 6 cut(s) 301, 587, 760, 821, 835, 892
FaqI GGGAC 4 cut(s) 21, 91, 242, 334
FatI CATG 6 cut(s) 297, 583, 756, 817, 831, 888
FauNDI CATATG 1 cut(s) 796
FblI GTMKAC 2 cut(s) 117, 249
FokI GGATG 3 cut(s) 3, 494, 550
FriOI GRGCYC 1 cut(s) 342
FspBI CTAG 2 cut(s) 507, 513
GlaI GCGC 5 cut(s) 66, 150, 242, 286, 304
GsaI CCCAGC 1 cut(s) 178
GsuI CTGGAG 3 cut(s) 71, 89, 849
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 3 cut(s) 173, 596, 767
HapII CCGG 3 cut(s) 34, 207, 753
HgaI GACGC 4 cut(s) 49, 239, 281, 791
HhaI GCGC 5 cut(s) 67, 151, 243, 287, 305
Hin1I GRCGYC 3 cut(s) 41, 231, 273
Hin1II CATG 6 cut(s) 301, 587, 760, 821, 835, 892
Hin6I GCGC 5 cut(s) 65, 149, 241, 285, 303
HinP1I GCGC 5 cut(s) 65, 149, 241, 285, 303
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HindIII AAGCTT 1 cut(s) 578
HinfI GANTC 2 cut(s) 293, 403
HpaII CCGG 3 cut(s) 34, 207, 753
Hpy166II GTNNAC 3 cut(s) 118, 250, 736
Hpy188I TCNGA 4 cut(s) 223, 318, 412, 879
Hpy188III TCNNGA 4 cut(s) 88, 351, 448, 566
Hpy8I GTNNAC 3 cut(s) 118, 250, 736
Hpy99I CGWCG 4 cut(s) 64, 119, 203, 206
HpyAV CCTTC 3 cut(s) 463, 471, 671
HpyCH4III ACNGT 2 cut(s) 77, 95
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 8 cut(s) 485, 602, 623, 632, 695, 760, 806, 835
HpyF3I CTNAG 1 cut(s) 405
HpySE526I ACGT 1 cut(s) 144
Hsp92I GRCGYC 3 cut(s) 41, 231, 273
Hsp92II CATG 6 cut(s) 301, 587, 760, 821, 835, 892
HspAI GCGC 5 cut(s) 65, 149, 241, 285, 303
KpnI GGTACC 1 cut(s) 691
KroI GCCGGC 1 cut(s) 752
KroNI GCCGGC 1 cut(s) 754
KspI CCGCGG 1 cut(s) 171
Kzo9I GATC 3 cut(s) 99, 381, 742
LmnI GCTCC 2 cut(s) 337, 358
LweI GCATC 2 cut(s) 472, 769
MaeI CTAG 2 cut(s) 507, 513
MaeII ACGT 1 cut(s) 144
MalI GATC 3 cut(s) 101, 383, 744
MboI GATC 3 cut(s) 99, 381, 742
MboII GAAGA 2 cut(s) 511, 619
MfeI CAATTG 1 cut(s) 486
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 2 cut(s) 342, 528
MlsI TGGCCA 1 cut(s) 596
MluCI AATT 2 cut(s) 486, 657
MluNI TGGCCA 1 cut(s) 596
MlyI GAGTC 2 cut(s) 287, 397
Mox20I TGGCCA 1 cut(s) 596
Mph1103I ATGCAT 1 cut(s) 762
MroNI GCCGGC 1 cut(s) 752
MscI TGGCCA 1 cut(s) 596
MseI TTAA 2 cut(s) 435, 468
Msp20I TGGCCA 1 cut(s) 596
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 3 cut(s) 34, 207, 753
MspR9I CCNGG 3 cut(s) 311, 684, 771
MunI CAATTG 1 cut(s) 486
MvaI CCWGG 3 cut(s) 311, 684, 771
MvnI CGCG 4 cut(s) 170, 243, 285, 567
NaeI GCCGGC 1 cut(s) 754
NcoI CCATGG 1 cut(s) 297
NdeI CATATG 1 cut(s) 796
NdeII GATC 3 cut(s) 99, 381, 742
NgoMIV GCCGGC 1 cut(s) 752
NheI GCTAGC 1 cut(s) 512
NlaIII CATG 6 cut(s) 301, 587, 760, 821, 835, 892
NlaIV GGNNCC 3 cut(s) 320, 689, 768
NmeAIII GCCGAG 3 cut(s) 259, 301, 321
NruI TCGCGA 1 cut(s) 567
NsiI ATGCAT 1 cut(s) 762
NspI RCATGY 3 cut(s) 760, 821, 835
PaeI GCATGC 1 cut(s) 760
PcsI WCGNNNNNNNCGW 1 cut(s) 56
PdiI GCCGGC 1 cut(s) 754
PflFI GACNNNGTC 1 cut(s) 406
Ple19I CGATCG 1 cut(s) 384
PleI GAGTC 2 cut(s) 287, 397
PpsI GAGTC 2 cut(s) 287, 397
PpuMI RGGWCCY 2 cut(s) 160, 680
Psp124BI GAGCTC 1 cut(s) 342
Psp5II RGGWCCY 2 cut(s) 160, 680
Psp6I CCWGG 3 cut(s) 309, 682, 769
PspFI CCCAGC 1 cut(s) 174
PspGI CCWGG 3 cut(s) 309, 682, 769
PspN4I GGNNCC 3 cut(s) 320, 689, 768
PspPI GGNCC 6 cut(s) 73, 160, 172, 318, 680, 766
PspPPI RGGWCCY 2 cut(s) 160, 680
PstI CTGCAG 3 cut(s) 625, 634, 697
PstNI CAGNNNCTG 2 cut(s) 629, 776
PsuI RGATCY 1 cut(s) 99
PsyI GACNNNGTC 1 cut(s) 406
PvuI CGATCG 1 cut(s) 384
RruI TCGCGA 1 cut(s) 567
RsaI GTAC 2 cut(s) 689, 737
RsaNI GTAC 2 cut(s) 688, 736
SacI GAGCTC 1 cut(s) 342
SacII CCGCGG 1 cut(s) 171
SalI GTCGAC 1 cut(s) 116
SaqAI TTAA 2 cut(s) 435, 468
Sau3AI GATC 3 cut(s) 99, 381, 742
Sau96I GGNCC 6 cut(s) 73, 160, 172, 318, 680, 766
SchI GAGTC 2 cut(s) 287, 397
ScrFI CCNGG 3 cut(s) 311, 684, 771
SduI GDGCHC 2 cut(s) 342, 528
SfaNI GCATC 2 cut(s) 472, 769
SfcI CTRYAG 3 cut(s) 621, 630, 693
Sfr303I CCGCGG 1 cut(s) 171
SgrBI CCGCGG 1 cut(s) 171
SinI GGWCC 4 cut(s) 73, 160, 318, 680
SmlI CTYRAG 2 cut(s) 152, 521
SmoI CTYRAG 2 cut(s) 152, 521
SphI GCATGC 1 cut(s) 760
Sse9I AATT 2 cut(s) 486, 657
SsiI CCGC 9 cut(s) 5, 138, 168, 170, 187, 365, 428, 554, 842
SspMI CTAG 2 cut(s) 507, 513
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 3 cut(s) 309, 682, 769
StyI CCWWGG 1 cut(s) 297
TaaI ACNGT 2 cut(s) 77, 95
TaiI ACGT 1 cut(s) 147
TaqI TCGA 5 cut(s) 22, 117, 291, 384, 447
TaqII GACCGA 1 cut(s) 40
TasI AATT 2 cut(s) 486, 657
TauI GCSGC 4 cut(s) 140, 170, 173, 557
Tru1I TTAA 2 cut(s) 435, 468
Tru9I TTAA 2 cut(s) 435, 468
TscAI CASTG 1 cut(s) 651
TspDTI ATGAA 3 cut(s) 572, 669, 690
TspGWI ACGGA 1 cut(s) 187
TspRI CASTG 1 cut(s) 651
Tth111I GACNNNGTC 1 cut(s) 406
VpaK11BI GGWCC 4 cut(s) 73, 160, 318, 680
XapI RAATTY 1 cut(s) 657
XceI RCATGY 3 cut(s) 760, 821, 835
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
XmiI GTMKAC 2 cut(s) 117, 249
XspI CTAG 2 cut(s) 507, 513
Zsp2I ATGCAT 1 cut(s) 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.