Rorug06G0254400

Protein FLC EXPRESSOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000946
Physical Location & Seq
Forward (+)
40078826 .. 40079868
1043 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0254400.1

Sequence Viewer

Length: 951 bp
ATGCCATCGTTTACGAAGCACATCTTCTCCACAATTTCCAACCCTATTGGCCATTCATCTCAGGCCTCCATTTCCGCTAGCTCGTTGATTAAACTACGTCGTTTTAGCTCCGGTACTGATCGTGGCCGTGAAATGGAAGCGCCGGAGAAACAACCGCCGGAGCCGATACCCAATAGGCCCTTAAGAGGGCAGCGACCATCGAATCCCCGGGCCAATGTTGATAGAAGGAGAGAGAGCCATCCCAATGTAGAAAGGAGGAGGGAGAACCCTAATCCGCCATTGCAGGACAGCAGTTTTCTTGAGAAGCTCAAGATGGGTCTTGACAAGTCGAAGAGAGAGAAGCCCCAGGAACCGGCGGAGGAGCCGCCGCCGCTGCAGCAGCCGCCGGAGGAGGCTAATGAGATATTCAAGAAGATGAAGGAGACGGGTCTGATTCCCAATGCAGTGGCCATGCTTGATGGGCTCTGCAAAGATGGGCTGGTCCAGGAGGCAATGAAGCTTTTCGGGTCGATGCGCGAAAAGGGTACTATTCCTGAGGTGGTGATATACACTGCTGTGGTGGAAGGGTTTTGCAAAGCCCAGAAGCCTGAAGATGCTAAGAGGATTTTCAGGAAGATGCAGAGCAATGGGATTGTTCCGAATGCGTTTAGTTACAATGTGTTGGTGCAGGGGTTGTGTAGGTGTGATAAGCTGGAGGATGCTGCTGAGTTCTGTGGCGAAATGTTGGAAGCCGGTCACTCGCCGAATGTGACTACTTTTGTGGGGTTGGTTGATGGGGTATGCAAGGAAAGTGGCGTGGAAGAAGCTGGAAGTGTGATTGGGAAGTTGAAGCAGAAGGGATATATGGTTAATGAGAAGGCTGTTAGAGAGTTTTTGGATAAAAGGGCAACATTTTCGCCAATGGTTTGGGAAGCTATCTTTGGGAAGAACCACCCAAAGAAACCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

316

Amino Acids

35.27

Weight (kDa)

9.02

Isoelectric Point (pI)

54.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 150 - 175 1.3e-06 PPR repeat
PPR_long PF17177 161 - 257 1e-05 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 167 - 222 5.6e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 177 - 207 2.4e-06 PPR repeat
PPR_2 PF13041 178 - 227 1.9e-15 PPR repeat family
PPR PF01535 181 - 211 1.8e-06 PPR repeat
PPR_3 PF13812 201 - 254 3e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 209 - 241 5.8e-09 PPR repeat
PPR_2 PF13041 213 - 262 3.7e-12 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 516
AciI CCGC 8 cut(s) 75, 155, 275, 356, 365, 368, 371, 383
AcoI YGGCCR 3 cut(s) 49, 124, 447
AcuI CTGAAG 1 cut(s) 609
AfaI GTAC 2 cut(s) 115, 526
AfiI CCNNNNNNNGG 4 cut(s) 133, 185, 186, 352
AflII CTTAAG 1 cut(s) 181
AgsI TTSAA 2 cut(s) 409, 829
AjnI CCWGG 2 cut(s) 345, 483
AjuI GAANNNNNNNTTGG 2 cut(s) 903, 935
AleI CACNNNNGTG 1 cut(s) 554
AluBI AGCT 7 cut(s) 81, 108, 307, 499, 691, 806, 914
AluI AGCT 7 cut(s) 81, 108, 307, 499, 691, 806, 914
Alw26I GTCTC 1 cut(s) 416
Ama87I CYCGRG 1 cut(s) 207
AoxI GGCC 6 cut(s) 49, 63, 124, 176, 210, 447
ApeKI GCWGC 5 cut(s) 190, 373, 376, 379, 701
Asp700I GAANNNNTTC 1 cut(s) 500
AspLEI GCGC 2 cut(s) 142, 516
AspS9I GGNCC 3 cut(s) 177, 210, 481
AsuC2I CCSGG 2 cut(s) 208, 209
AsuHPI GGTGA 1 cut(s) 553
AsuNHI GCTAGC 1 cut(s) 77
AvaI CYCGRG 1 cut(s) 207
AvaII GGWCC 1 cut(s) 481
AxyI CCTNAGG 1 cut(s) 534
BalI TGGCCA 2 cut(s) 51, 449
BanII GRGCYC 1 cut(s) 465
BbvI GCAGC 5 cut(s) 202, 360, 388, 391, 688
BccI CCATC 7 cut(s) 13, 205, 246, 307, 452, 467, 767
BceAI ACGGC 1 cut(s) 111
BcgI CGANNNNNNTGC 2 cut(s) 876, 910
BciT130I CCWGG 2 cut(s) 347, 485
BcnI CCSGG 2 cut(s) 208, 209
BcoDI GTCTC 1 cut(s) 416
BfaI CTAG 1 cut(s) 78
BfmI CTRYAG 1 cut(s) 374
BfoI RGCGCY 1 cut(s) 143
BfrI CTTAAG 1 cut(s) 181
BisI GCNGC 9 cut(s) 191, 365, 368, 371, 374, 377, 380, 383, 702
BlsI GCNGC 9 cut(s) 192, 366, 369, 372, 375, 378, 381, 384, 703
Bme1390I CCNGG 4 cut(s) 208, 209, 347, 485
Bme18I GGWCC 1 cut(s) 481
BmeT110I CYCGRG 1 cut(s) 207
BmgT120I GGNCC 3 cut(s) 177, 210, 481
BmiI GGNNCC 3 cut(s) 162, 351, 363
BmrFI CCNGG 4 cut(s) 208, 209, 347, 485
BmsI GCATC 4 cut(s) 501, 583, 606, 688
BmtI GCTAGC 1 cut(s) 81
BpmI CTGGAG 1 cut(s) 713
BpuEI CTTGAG 2 cut(s) 293, 320
BpuMI CCSGG 2 cut(s) 208, 209
BsaJI CCNNGG 3 cut(s) 206, 207, 345
BsaWI WCCGGW 1 cut(s) 110
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 4 cut(s) 133, 185, 186, 352
Bse118I RCCGGY 2 cut(s) 352, 731
Bse21I CCTNAGG 1 cut(s) 534
Bse3DI GCAATG 3 cut(s) 278, 498, 631
BseBI CCWGG 2 cut(s) 347, 485
BseDI CCNNGG 3 cut(s) 206, 207, 345
BseGI GGATG 2 cut(s) 238, 703
BseLI CCNNNNNNNGG 4 cut(s) 133, 185, 186, 352
BseMI GCAATG 3 cut(s) 278, 498, 631
BseMII CTCAG 3 cut(s) 74, 525, 696
BseRI GAGGAG 3 cut(s) 271, 374, 404
BseXI GCAGC 5 cut(s) 202, 360, 388, 391, 688
BsgI GTGCAG 1 cut(s) 686
Bsh1236I CGCG 1 cut(s) 516
BshFI GGCC 6 cut(s) 51, 65, 126, 178, 212, 449
BsiHKCI CYCGRG 1 cut(s) 207
BsiSI CCGG 7 cut(s) 111, 143, 158, 208, 353, 386, 732
BslI CCNNNNNNNGG 4 cut(s) 133, 185, 186, 352
BsmAI GTCTC 1 cut(s) 416
BsmBI CGTCTC 1 cut(s) 416
BsmI GAATGC 1 cut(s) 646
BsnI GGCC 6 cut(s) 51, 65, 126, 178, 212, 449
BsoBI CYCGRG 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 465
Bsp143I GATC 1 cut(s) 118
BspACI CCGC 8 cut(s) 75, 155, 275, 356, 365, 368, 371, 383
BspANI GGCC 6 cut(s) 51, 65, 126, 178, 212, 449
BspCNI CTCAG 3 cut(s) 73, 526, 697
BspFNI CGCG 1 cut(s) 516
BspLI GGNNCC 3 cut(s) 162, 351, 363
BspMAI CTGCAG 1 cut(s) 378
BspOI GCTAGC 1 cut(s) 81
BspTI CTTAAG 1 cut(s) 181
BsrDI GCAATG 3 cut(s) 278, 498, 631
BsrFI RCCGGY 2 cut(s) 352, 731
BssAI RCCGGY 2 cut(s) 352, 731
BssECI CCNNGG 3 cut(s) 206, 207, 345
BssMI GATC 1 cut(s) 118
Bst2UI CCWGG 2 cut(s) 347, 485
Bst6I CTCTTC 1 cut(s) 326
BstAFI CTTAAG 1 cut(s) 181
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 4 cut(s) 60, 534, 597, 705
BstF5I GGATG 2 cut(s) 238, 703
BstFNI CGCG 1 cut(s) 516
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 2 cut(s) 142, 516
BstKTI GATC 1 cut(s) 121
BstMAI GTCTC 1 cut(s) 416
BstMBI GATC 1 cut(s) 118
BstMWI GCNNNNNNNGC 6 cut(s) 370, 373, 376, 379, 382, 460
BstNI CCWGG 2 cut(s) 347, 485
BstSCI CCNGG 4 cut(s) 206, 207, 345, 483
BstSFI CTRYAG 1 cut(s) 374
BstUI CGCG 1 cut(s) 516
BstV1I GCAGC 5 cut(s) 202, 360, 388, 391, 688
BstXI CCANNNNNNTGG 2 cut(s) 445, 906
Bsu36I CCTNAGG 1 cut(s) 534
BsuRI GGCC 6 cut(s) 51, 65, 126, 178, 212, 449
BtsCI GGATG 2 cut(s) 238, 703
BtsI GCAGTG 2 cut(s) 450, 549
BtsIMutI CAGTG 2 cut(s) 450, 549
Cac8I GCNNGC 1 cut(s) 79
CfoI GCGC 2 cut(s) 142, 516
Cfr10I RCCGGY 2 cut(s) 352, 731
Cfr13I GGNCC 3 cut(s) 177, 210, 481
Cfr9I CCCGGG 1 cut(s) 207
Csp6I GTAC 2 cut(s) 114, 525
CspCI CAANNNNNGTGG 2 cut(s) 772, 807
CviAII CATG 1 cut(s) 451
CviQI GTAC 2 cut(s) 114, 525
DdeI CTNAG 4 cut(s) 60, 534, 597, 705
DpnI GATC 1 cut(s) 120
DpnII GATC 1 cut(s) 118
EaeI YGGCCR 3 cut(s) 49, 124, 447
Eam1104I CTCTTC 1 cut(s) 326
EarI CTCTTC 1 cut(s) 326
EciI GGCGGA 2 cut(s) 264, 371
Eco147I AGGCCT 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 465
Eco47I GGWCC 1 cut(s) 481
Eco57I CTGAAG 1 cut(s) 609
Eco81I CCTNAGG 1 cut(s) 534
Eco88I CYCGRG 1 cut(s) 207
EcoO109I RGGNCCY 1 cut(s) 177
EcoRII CCWGG 2 cut(s) 345, 483
EcoT38I GRGCYC 1 cut(s) 465
Esp3I CGTCTC 1 cut(s) 416
FaeI CATG 1 cut(s) 454
FaiI YATR 5 cut(s) 452, 547, 781, 843, 845
FalI AAGNNNNNCTT 2 cut(s) 8, 40
FatI CATG 1 cut(s) 450
Fnu4HI GCNGC 9 cut(s) 191, 365, 368, 371, 374, 377, 380, 383, 702
FokI GGATG 2 cut(s) 225, 710
FriOI GRGCYC 1 cut(s) 465
Fsp4HI GCNGC 9 cut(s) 191, 365, 368, 371, 374, 377, 380, 383, 702
FspBI CTAG 1 cut(s) 78
GlaI GCGC 2 cut(s) 141, 515
GluI GCNGC 9 cut(s) 191, 365, 368, 371, 374, 377, 380, 383, 702
GsuI CTGGAG 1 cut(s) 713
HaeII RGCGCY 1 cut(s) 143
HaeIII GGCC 6 cut(s) 51, 65, 126, 178, 212, 449
HapII CCGG 7 cut(s) 111, 143, 158, 208, 353, 386, 732
HhaI GCGC 2 cut(s) 142, 516
Hin1II CATG 1 cut(s) 454
Hin6I GCGC 2 cut(s) 140, 514
HinP1I GCGC 2 cut(s) 140, 514
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 2 cut(s) 202, 433
HpaII CCGG 7 cut(s) 111, 143, 158, 208, 353, 386, 732
HphI GGTGA 1 cut(s) 553
Hpy166II GTNNAC 1 cut(s) 12
Hpy188I TCNGA 2 cut(s) 432, 639
Hpy188III TCNNGA 6 cut(s) 299, 310, 320, 409, 533, 610
Hpy8I GTNNAC 1 cut(s) 12
Hpy99I CGWCG 1 cut(s) 102
HpyAV CCTTC 5 cut(s) 219, 412, 557, 829, 850
HpyCH4IV ACGT 1 cut(s) 97
HpyCH4V TGCA 8 cut(s) 283, 376, 443, 468, 573, 619, 667, 783
HpyF10VI GCNNNNNNNGC 6 cut(s) 370, 373, 376, 379, 382, 460
HpyF3I CTNAG 4 cut(s) 60, 534, 597, 705
HpySE526I ACGT 1 cut(s) 97
Hsp92II CATG 1 cut(s) 454
HspAI GCGC 2 cut(s) 140, 514
Kzo9I GATC 1 cut(s) 118
LmnI GCTCC 3 cut(s) 113, 160, 361
Lsp1109I GCAGC 5 cut(s) 202, 360, 388, 391, 688
LweI GCATC 4 cut(s) 501, 583, 606, 688
MaeI CTAG 1 cut(s) 78
MaeII ACGT 1 cut(s) 97
MaeIII GTNAC 3 cut(s) 650, 734, 748
MalI GATC 1 cut(s) 120
MboI GATC 1 cut(s) 118
MboII GAAGA 7 cut(s) 16, 343, 424, 602, 625, 812, 937
MhlI GDGCHC 1 cut(s) 465
MlsI TGGCCA 2 cut(s) 51, 449
MluCI AATT 1 cut(s) 33
MluNI TGGCCA 2 cut(s) 51, 449
MmeI TCCRAC 2 cut(s) 63, 705
Mox20I TGGCCA 2 cut(s) 51, 449
MroXI GAANNNNTTC 1 cut(s) 500
MscI TGGCCA 2 cut(s) 51, 449
MseI TTAA 3 cut(s) 90, 182, 849
MslI CAYNNNNRTG 2 cut(s) 243, 554
Msp20I TGGCCA 2 cut(s) 51, 449
MspA1I CMGCKG 1 cut(s) 373
MspCI CTTAAG 1 cut(s) 181
MspI CCGG 7 cut(s) 111, 143, 158, 208, 353, 386, 732
MspR9I CCNGG 4 cut(s) 208, 209, 347, 485
Mva1269I GAATGC 1 cut(s) 646
MvaI CCWGG 2 cut(s) 347, 485
MvnI CGCG 1 cut(s) 516
MwoI GCNNNNNNNGC 6 cut(s) 370, 373, 376, 379, 382, 460
NciI CCSGG 2 cut(s) 208, 209
NdeII GATC 1 cut(s) 118
NheI GCTAGC 1 cut(s) 77
NlaIII CATG 1 cut(s) 454
NlaIV GGNNCC 3 cut(s) 162, 351, 363
NmuCI GTSAC 2 cut(s) 734, 748
OliI CACNNNNGTG 1 cut(s) 554
PceI AGGCCT 1 cut(s) 65
PctI GAATGC 1 cut(s) 646
PdmI GAANNNNTTC 1 cut(s) 500
PfeI GAWTC 2 cut(s) 202, 433
PfoI TCCNGGA 1 cut(s) 483
PkrI GCNGC 9 cut(s) 192, 366, 369, 372, 375, 378, 381, 384, 703
Psp6I CCWGG 2 cut(s) 345, 483
PspGI CCWGG 2 cut(s) 345, 483
PspN4I GGNNCC 3 cut(s) 162, 351, 363
PspPI GGNCC 3 cut(s) 177, 210, 481
PstI CTGCAG 1 cut(s) 378
RsaI GTAC 2 cut(s) 115, 526
RsaNI GTAC 2 cut(s) 114, 525
RseI CAYNNNNRTG 2 cut(s) 243, 554
SaqAI TTAA 3 cut(s) 90, 182, 849
SatI GCNGC 9 cut(s) 191, 365, 368, 371, 374, 377, 380, 383, 702
Sau3AI GATC 1 cut(s) 118
Sau96I GGNCC 3 cut(s) 177, 210, 481
ScrFI CCNGG 4 cut(s) 208, 209, 347, 485
SduI GDGCHC 1 cut(s) 465
SfaNI GCATC 4 cut(s) 501, 583, 606, 688
SfcI CTRYAG 1 cut(s) 374
SinI GGWCC 1 cut(s) 481
SmaI CCCGGG 1 cut(s) 209
SmiMI CAYNNNNRTG 2 cut(s) 243, 554
SmlI CTYRAG 3 cut(s) 181, 299, 308
SmoI CTYRAG 3 cut(s) 181, 299, 308
Sse9I AATT 1 cut(s) 33
SseBI AGGCCT 1 cut(s) 65
SsiI CCGC 8 cut(s) 75, 155, 275, 356, 365, 368, 371, 383
SspMI CTAG 1 cut(s) 78
StuI AGGCCT 1 cut(s) 65
StyD4I CCNGG 4 cut(s) 206, 207, 345, 483
TaiI ACGT 1 cut(s) 100
TaqI TCGA 3 cut(s) 200, 329, 509
TasI AATT 1 cut(s) 33
TauI GCSGC 4 cut(s) 367, 370, 373, 385
TfiI GAWTC 2 cut(s) 202, 433
Tru1I TTAA 3 cut(s) 90, 182, 849
Tru9I TTAA 3 cut(s) 90, 182, 849
TscAI CASTG 2 cut(s) 450, 556
TseFI GTSAC 2 cut(s) 734, 748
TseI GCWGC 5 cut(s) 190, 373, 376, 379, 701
Tsp45I GTSAC 2 cut(s) 734, 748
TspDTI ATGAA 3 cut(s) 45, 431, 509
TspMI CCCGGG 1 cut(s) 207
TspRI CASTG 2 cut(s) 450, 556
Vha464I CTTAAG 1 cut(s) 181
VpaK11BI GGWCC 1 cut(s) 481
XmaI CCCGGG 1 cut(s) 207
XmnI GAANNNNTTC 1 cut(s) 500
XspI CTAG 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.