MD03G1060200.v1.1

Protein FLC EXPRESSOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000946
Physical Location & Seq
Forward (+)
4864166 .. 4866377
2212 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1060200.v1.1.491

Sequence Viewer

Length: 831 bp
ATGGCGGGACGGGAACATCCTCGACAGCTCGTCCGGCTGACACCACTATCGGTCAATACCACGGCGCTGGAGGACCGTATCGCCCTCCAGAACCGTGAGATCCAGTCCCTCCTCGTCGACAATCAACGGCTGGCAGCCGCCCACGTCGCGCTCAAGCAGGACCTCGCCGCGGCCCAGCACGACCTACGCCATCTATCCGTCGTCGCCGGCAAAGCCAAATCTGAGAGGGACGCCGAGGTGCGCGAGGTCTACGAGAGGGCATTGAAGCTGGACGCCGAGGTTCGCGCCATCGACTCCATGGGCGCAGACCTGGCTCGGACCCGAGCCGATATACAGGAGCTCGGCTCGTCCCGGCAGGAGCTTGCAGAGGAGTTGAACACGATCGAAGGCGAGCTTGTGAGGACTCAGTCCGAGGCGAGGAAAGTGGTGGACATTAAAGCCGATATCGAGACCTTCAAGCAGGAGATTAAGAAGGGAAGGGATGCAATTGACAATGAGAAGAAAACTAGAGCTAGCAACCTTGAGCACAGGCAGGGGATGGAGAAAACAATGGCGGCATTGGCTCGCGAAATGGATAAGCTTCATGGGGAGTTGGCCAATGCAGAGAAGAGAGCAAGGGCTGCAGTAGCTGCAGCTGCAGCAGCAAATCCAGGTCCTGGGTACCCTGCAGCCTATGGCAATGCTGAAATGTTATACGGGGGAAATGCGTATCCCGATCCCTATGCCGGCATGCATCAGGCCGCAGGTACTGCCGACGCTGCGAGTCCATATGGTTCTGCACCAATGCCACATGCTTCTTATGACAGGCAGCCACCGCAGGATATGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

277

Amino Acids

30.11

Weight (kDa)

6.1

Isoelectric Point (pI)

34.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 734
Acc65I GGTACC 1 cut(s) 660
AccB1I GGYRCC 1 cut(s) 660
AccB7I CCANNNNNTGG 1 cut(s) 656
AccI GTMKAC 2 cut(s) 117, 249
AccII CGCG 5 cut(s) 149, 170, 243, 285, 567
AciI CCGC 7 cut(s) 5, 138, 168, 170, 554, 741, 815
AclWI GGATC 2 cut(s) 94, 710
AcoI YGGCCR 1 cut(s) 594
AcyI GRCGYC 2 cut(s) 231, 273
AfaI GTAC 2 cut(s) 662, 748
AfiI CCNNNNNNNGG 4 cut(s) 169, 417, 656, 725
AgsI TTSAA 3 cut(s) 265, 376, 457
AhdI GACNNNNNGTC 1 cut(s) 29
AjiI CACGTC 1 cut(s) 145
AjnI CCWGG 3 cut(s) 309, 649, 655
AluBI AGCT 9 cut(s) 28, 268, 340, 361, 394, 512, 580, 629, 635
AluI AGCT 9 cut(s) 28, 268, 340, 361, 394, 512, 580, 629, 635
Alw21I GWGCWC 2 cut(s) 342, 528
Alw26I GTCTC 1 cut(s) 443
AlwI GGATC 2 cut(s) 94, 710
AlwNI CAGNNNCTG 3 cut(s) 629, 656, 749
Ama87I CYCGRG 1 cut(s) 321
AoxI GGCC 3 cut(s) 171, 594, 738
Asp718I GGTACC 1 cut(s) 660
AspLEI GCGC 5 cut(s) 67, 151, 243, 287, 305
AspS9I GGNCC 5 cut(s) 73, 160, 172, 318, 653
AsuC2I CCSGG 1 cut(s) 352
AsuNHI GCTAGC 1 cut(s) 512
AvaI CYCGRG 1 cut(s) 321
AvaII GGWCC 4 cut(s) 73, 160, 318, 653
BalI TGGCCA 1 cut(s) 596
BanI GGYRCC 1 cut(s) 660
BanII GRGCYC 1 cut(s) 342
Bbv12I GWGCWC 2 cut(s) 342, 528
BccI CCATC 3 cut(s) 198, 296, 532
BceAI ACGGC 2 cut(s) 78, 143
BcgI CGANNNNNNTGC 2 cut(s) 704, 738
BciT130I CCWGG 3 cut(s) 311, 651, 657
BciVI GTATCC 1 cut(s) 720
BcnI CCSGG 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 443
BfaI CTAG 2 cut(s) 507, 513
BfmI CTRYAG 4 cut(s) 621, 630, 636, 666
BfoI RGCGCY 1 cut(s) 68
BfuAI ACCTGC 1 cut(s) 734
BfuI GTATCC 1 cut(s) 720
Bme1390I CCNGG 4 cut(s) 311, 352, 651, 657
Bme18I GGWCC 4 cut(s) 73, 160, 318, 653
BmeRI GACNNNNNGTC 1 cut(s) 29
BmeT110I CYCGRG 1 cut(s) 321
BmgBI CACGTC 1 cut(s) 145
BmgT120I GGNCC 5 cut(s) 73, 160, 172, 318, 653
BmiI GGNNCC 2 cut(s) 320, 662
BmrFI CCNGG 4 cut(s) 311, 352, 651, 657
BmsI GCATC 2 cut(s) 472, 742
BmtI GCTAGC 1 cut(s) 516
BplI GAGNNNNNCTC 2 cut(s) 329, 361
BpmI CTGGAG 2 cut(s) 71, 89
BpuEI CTTGAG 2 cut(s) 137, 542
BpuMI CCSGG 1 cut(s) 352
BsaHI GRCGYC 2 cut(s) 231, 273
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 7 cut(s) 60, 168, 234, 276, 297, 411, 656
Bsc4I CCNNNNNNNGG 4 cut(s) 169, 417, 656, 725
Bse118I RCCGGY 2 cut(s) 206, 725
Bse1I ACTGG 1 cut(s) 103
Bse3DI GCAATG 1 cut(s) 685
BseBI CCWGG 3 cut(s) 311, 651, 657
BseDI CCNNGG 7 cut(s) 60, 168, 234, 276, 297, 411, 656
BseGI GGATG 3 cut(s) 16, 487, 543
BseLI CCNNNNNNNGG 4 cut(s) 169, 417, 656, 725
BseMI GCAATG 1 cut(s) 685
BseMII CTCAG 2 cut(s) 213, 419
BseNI ACTGG 1 cut(s) 103
BseRI GAGGAG 2 cut(s) 101, 383
BseYI CCCAGC 1 cut(s) 174
BsgI GTGCAG 1 cut(s) 762
Bsh1236I CGCG 5 cut(s) 149, 170, 243, 285, 567
Bsh1285I CGRYCG 1 cut(s) 384
BshFI GGCC 3 cut(s) 173, 596, 740
BshNI GGYRCC 1 cut(s) 660
BsiEI CGRYCG 1 cut(s) 384
BsiHKAI GWGCWC 2 cut(s) 342, 528
BsiHKCI CYCGRG 1 cut(s) 321
BsiSI CCGG 4 cut(s) 34, 207, 352, 726
BslFI GGGAC 4 cut(s) 21, 91, 242, 334
BslI CCNNNNNNNGG 4 cut(s) 169, 417, 656, 725
BsmAI GTCTC 1 cut(s) 443
BsmFI GGGAC 4 cut(s) 21, 91, 242, 334
BsnI GGCC 3 cut(s) 173, 596, 740
Bso31I GGTCTC 1 cut(s) 443
BsoBI CYCGRG 1 cut(s) 321
Bsp1286I GDGCHC 2 cut(s) 342, 528
Bsp143I GATC 3 cut(s) 99, 381, 715
Bsp19I CCATGG 1 cut(s) 297
Bsp68I TCGCGA 1 cut(s) 567
BspACI CCGC 7 cut(s) 5, 138, 168, 170, 554, 741, 815
BspANI GGCC 3 cut(s) 173, 596, 740
BspCNI CTCAG 2 cut(s) 214, 418
BspFNI CGCG 5 cut(s) 149, 170, 243, 285, 567
BspLI GGNNCC 2 cut(s) 320, 662
BspMAI CTGCAG 4 cut(s) 625, 634, 640, 670
BspMI ACCTGC 1 cut(s) 734
BspOI GCTAGC 1 cut(s) 516
BspPI GGATC 2 cut(s) 94, 710
BspT107I GGYRCC 1 cut(s) 660
BspTNI GGTCTC 1 cut(s) 443
BsrDI GCAATG 1 cut(s) 685
BsrFI RCCGGY 2 cut(s) 206, 725
BsrI ACTGG 1 cut(s) 103
BssAI RCCGGY 2 cut(s) 206, 725
BssECI CCNNGG 7 cut(s) 60, 168, 234, 276, 297, 411, 656
BssMI GATC 3 cut(s) 99, 381, 715
BssNI GRCGYC 2 cut(s) 231, 273
BssT1I CCWWGG 1 cut(s) 297
Bst2UI CCWGG 3 cut(s) 311, 651, 657
Bst4CI ACNGT 2 cut(s) 77, 95
Bst6I CTCTTC 1 cut(s) 602
BstACI GRCGYC 2 cut(s) 231, 273
BstAPI GCANNNNNTGC 3 cut(s) 620, 629, 749
BstC8I GCNNGC 8 cut(s) 132, 208, 363, 392, 514, 565, 727, 731
BstDEI CTNAG 2 cut(s) 222, 405
BstDSI CCRYGG 3 cut(s) 60, 168, 297
BstF5I GGATG 3 cut(s) 16, 487, 543
BstFNI CGCG 5 cut(s) 149, 170, 243, 285, 567
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 5 cut(s) 67, 151, 243, 287, 305
BstKTI GATC 3 cut(s) 102, 384, 718
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 3 cut(s) 99, 381, 715
BstMCI CGRYCG 1 cut(s) 384
BstNI CCWGG 3 cut(s) 311, 651, 657
BstNSI RCATGY 2 cut(s) 733, 794
BstSCI CCNGG 4 cut(s) 309, 350, 649, 655
BstSFI CTRYAG 4 cut(s) 621, 630, 636, 666
BstUI CGCG 5 cut(s) 149, 170, 243, 285, 567
BstX2I RGATCY 1 cut(s) 99
BstXI CCANNNNNNTGG 1 cut(s) 67
BstYI RGATCY 1 cut(s) 99
BsuI GTATCC 1 cut(s) 720
BsuRI GGCC 3 cut(s) 173, 596, 740
BtgI CCRYGG 3 cut(s) 60, 168, 297
BtrI CACGTC 1 cut(s) 145
BtsCI GGATG 3 cut(s) 16, 487, 543
BtuMI TCGCGA 1 cut(s) 567
BveI ACCTGC 1 cut(s) 734
Cac8I GCNNGC 8 cut(s) 132, 208, 363, 392, 514, 565, 727, 731
CaiI CAGNNNCTG 3 cut(s) 629, 656, 749
CfoI GCGC 5 cut(s) 67, 151, 243, 287, 305
Cfr10I RCCGGY 2 cut(s) 206, 725
Cfr13I GGNCC 5 cut(s) 73, 160, 172, 318, 653
Cfr42I CCGCGG 1 cut(s) 171
CseI GACGC 3 cut(s) 239, 281, 764
Csp6I GTAC 2 cut(s) 661, 747
CviAII CATG 4 cut(s) 298, 584, 730, 791
CviQI GTAC 2 cut(s) 661, 747
DdeI CTNAG 2 cut(s) 222, 405
DpnI GATC 3 cut(s) 101, 383, 717
DpnII GATC 3 cut(s) 99, 381, 715
DriI GACNNNNNGTC 1 cut(s) 29
EaeI YGGCCR 1 cut(s) 594
Eam1104I CTCTTC 1 cut(s) 602
Eam1105I GACNNNNNGTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 602
Ecl136II GAGCTC 1 cut(s) 340
Eco130I CCWWGG 1 cut(s) 297
Eco24I GRGCYC 1 cut(s) 342
Eco31I GGTCTC 1 cut(s) 443
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 4 cut(s) 73, 160, 318, 653
Eco53kI GAGCTC 1 cut(s) 340
Eco88I CYCGRG 1 cut(s) 321
EcoICRI GAGCTC 1 cut(s) 340
EcoO109I RGGNCCY 2 cut(s) 160, 653
EcoRII CCWGG 3 cut(s) 309, 649, 655
EcoRV GATATC 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 297
EcoT22I ATGCAT 1 cut(s) 735
EcoT38I GRGCYC 1 cut(s) 342
ErhI CCWWGG 1 cut(s) 297
FaeI CATG 4 cut(s) 301, 587, 733, 794
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FaqI GGGAC 4 cut(s) 21, 91, 242, 334
FatI CATG 4 cut(s) 297, 583, 729, 790
FauNDI CATATG 1 cut(s) 769
FblI GTMKAC 2 cut(s) 117, 249
FokI GGATG 3 cut(s) 3, 494, 550
FriOI GRGCYC 1 cut(s) 342
FspBI CTAG 2 cut(s) 507, 513
GlaI GCGC 5 cut(s) 66, 150, 242, 286, 304
GsaI CCCAGC 1 cut(s) 178
GsuI CTGGAG 2 cut(s) 71, 89
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 3 cut(s) 173, 596, 740
HapII CCGG 4 cut(s) 34, 207, 352, 726
HgaI GACGC 3 cut(s) 239, 281, 764
HhaI GCGC 5 cut(s) 67, 151, 243, 287, 305
Hin1I GRCGYC 2 cut(s) 231, 273
Hin1II CATG 4 cut(s) 301, 587, 733, 794
Hin6I GCGC 5 cut(s) 65, 149, 241, 285, 303
HinP1I GCGC 5 cut(s) 65, 149, 241, 285, 303
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HindIII AAGCTT 1 cut(s) 578
HinfI GANTC 3 cut(s) 293, 403, 763
HpaII CCGG 4 cut(s) 34, 207, 352, 726
Hpy166II GTNNAC 3 cut(s) 118, 250, 430
Hpy188I TCNGA 3 cut(s) 223, 318, 412
Hpy188III TCNNGA 4 cut(s) 88, 448, 566, 713
Hpy8I GTNNAC 3 cut(s) 118, 250, 430
Hpy99I CGWCG 5 cut(s) 119, 149, 203, 206, 758
HpyAV CCTTC 4 cut(s) 380, 463, 466, 471
HpyCH4III ACNGT 2 cut(s) 77, 95
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 9 cut(s) 365, 485, 602, 623, 632, 638, 668, 733, 779
HpyF3I CTNAG 2 cut(s) 222, 405
HpySE526I ACGT 1 cut(s) 144
Hsp92I GRCGYC 2 cut(s) 231, 273
Hsp92II CATG 4 cut(s) 301, 587, 733, 794
HspAI GCGC 5 cut(s) 65, 149, 241, 285, 303
KpnI GGTACC 1 cut(s) 664
KroI GCCGGC 2 cut(s) 206, 725
KroNI GCCGGC 2 cut(s) 208, 727
KspI CCGCGG 1 cut(s) 171
Kzo9I GATC 3 cut(s) 99, 381, 715
LmnI GCTCC 2 cut(s) 337, 358
LweI GCATC 2 cut(s) 472, 742
MaeI CTAG 2 cut(s) 507, 513
MaeII ACGT 1 cut(s) 144
MalI GATC 3 cut(s) 101, 383, 717
MboI GATC 3 cut(s) 99, 381, 715
MboII GAAGA 2 cut(s) 511, 619
MfeI CAATTG 1 cut(s) 486
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 2 cut(s) 342, 528
MlsI TGGCCA 1 cut(s) 596
MluCI AATT 1 cut(s) 486
MluNI TGGCCA 1 cut(s) 596
MlyI GAGTC 3 cut(s) 287, 397, 772
Mox20I TGGCCA 1 cut(s) 596
Mph1103I ATGCAT 1 cut(s) 735
MroNI GCCGGC 2 cut(s) 206, 725
MscI TGGCCA 1 cut(s) 596
MseI TTAA 2 cut(s) 435, 468
Msp20I TGGCCA 1 cut(s) 596
MspA1I CMGCKG 2 cut(s) 170, 635
MspI CCGG 4 cut(s) 34, 207, 352, 726
MspR9I CCNGG 4 cut(s) 311, 352, 651, 657
MunI CAATTG 1 cut(s) 486
MvaI CCWGG 3 cut(s) 311, 651, 657
MvnI CGCG 5 cut(s) 149, 170, 243, 285, 567
NaeI GCCGGC 2 cut(s) 208, 727
NciI CCSGG 1 cut(s) 352
NcoI CCATGG 1 cut(s) 297
NdeI CATATG 1 cut(s) 769
NdeII GATC 3 cut(s) 99, 381, 715
NgoMIV GCCGGC 2 cut(s) 206, 725
NheI GCTAGC 1 cut(s) 512
NlaIII CATG 4 cut(s) 301, 587, 733, 794
NlaIV GGNNCC 2 cut(s) 320, 662
NmeAIII GCCGAG 3 cut(s) 259, 301, 321
NruI TCGCGA 1 cut(s) 567
NsiI ATGCAT 1 cut(s) 735
NspI RCATGY 2 cut(s) 733, 794
PaeI GCATGC 1 cut(s) 733
PdiI GCCGGC 2 cut(s) 208, 727
PflFI GACNNNGTC 1 cut(s) 406
PflMI CCANNNNNTGG 1 cut(s) 656
Ple19I CGATCG 1 cut(s) 384
PleI GAGTC 3 cut(s) 287, 397, 771
PpsI GAGTC 3 cut(s) 287, 397, 771
PpuMI RGGWCCY 2 cut(s) 160, 653
Psp124BI GAGCTC 1 cut(s) 342
Psp5II RGGWCCY 2 cut(s) 160, 653
Psp6I CCWGG 3 cut(s) 309, 649, 655
PspFI CCCAGC 1 cut(s) 174
PspGI CCWGG 3 cut(s) 309, 649, 655
PspN4I GGNNCC 2 cut(s) 320, 662
PspPI GGNCC 5 cut(s) 73, 160, 172, 318, 653
PspPPI RGGWCCY 2 cut(s) 160, 653
PstI CTGCAG 4 cut(s) 625, 634, 640, 670
PstNI CAGNNNCTG 3 cut(s) 629, 656, 749
PsuI RGATCY 1 cut(s) 99
PsyI GACNNNGTC 1 cut(s) 406
PvuI CGATCG 1 cut(s) 384
PvuII CAGCTG 1 cut(s) 635
RruI TCGCGA 1 cut(s) 567
RsaI GTAC 2 cut(s) 662, 748
RsaNI GTAC 2 cut(s) 661, 747
SacI GAGCTC 1 cut(s) 342
SacII CCGCGG 1 cut(s) 171
SalI GTCGAC 1 cut(s) 116
SaqAI TTAA 2 cut(s) 435, 468
Sau3AI GATC 3 cut(s) 99, 381, 715
Sau96I GGNCC 5 cut(s) 73, 160, 172, 318, 653
SchI GAGTC 3 cut(s) 287, 397, 772
ScrFI CCNGG 4 cut(s) 311, 352, 651, 657
SduI GDGCHC 2 cut(s) 342, 528
SfaNI GCATC 2 cut(s) 472, 742
SfcI CTRYAG 4 cut(s) 621, 630, 636, 666
Sfr303I CCGCGG 1 cut(s) 171
SgrBI CCGCGG 1 cut(s) 171
SinI GGWCC 4 cut(s) 73, 160, 318, 653
SmlI CTYRAG 2 cut(s) 152, 521
SmoI CTYRAG 2 cut(s) 152, 521
SphI GCATGC 1 cut(s) 733
Sse9I AATT 1 cut(s) 486
SsiI CCGC 7 cut(s) 5, 138, 168, 170, 554, 741, 815
SspMI CTAG 2 cut(s) 507, 513
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 4 cut(s) 309, 350, 649, 655
StyI CCWWGG 1 cut(s) 297
TaaI ACNGT 2 cut(s) 77, 95
TaiI ACGT 1 cut(s) 147
TaqI TCGA 5 cut(s) 22, 117, 291, 384, 447
TaqII GACCGA 1 cut(s) 40
TasI AATT 1 cut(s) 486
TauI GCSGC 5 cut(s) 140, 170, 173, 557, 743
Tru1I TTAA 2 cut(s) 435, 468
Tru9I TTAA 2 cut(s) 435, 468
TspDTI ATGAA 1 cut(s) 572
TspGWI ACGGA 1 cut(s) 187
Tth111I GACNNNGTC 1 cut(s) 406
Van91I CCANNNNNTGG 1 cut(s) 656
VpaK11BI GGWCC 4 cut(s) 73, 160, 318, 653
XceI RCATGY 2 cut(s) 733, 794
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
XmiI GTMKAC 2 cut(s) 117, 249
XspI CTAG 2 cut(s) 507, 513
Zsp2I ATGCAT 1 cut(s) 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.