Rh2DG467400

Protein FLC EXPRESSOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000946
Physical Location & Seq
Forward (+)
67687954 .. 67700402
12449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG467400.1

Sequence Viewer

Length: 549 bp
ATGAACGCCGAGTTGACTCTGGTCCAGAATGACATCGAGGACTTGAGCACGTCGCGGATAGAGCTCACGACCGATTTGAACACGATCCAAGGAGAGATTGAGAGGGCTAGCTCCGACGAGTCGAAGCAAATCGAAGTCATTAGAGACGAAATTGAGACTTTGAAGCTGGAGATTGAGAAAGGAAGAAGGATAACAGTGCCAGAATTCACCGAATCAAGTTACAGAGTGGAGACCGGAGGTTCCAGGCTTCTGGTCATCGAAGGTCATCTTCCGGATCCACGACCTCAGCTAGCTGAAGCTTCTCGACTACGGCTGCACTACGCTTTGGACTCGTTGGCTTTCGACCGCGTTCGACCACTAGTACAACAGACCTCGTTCGCGCCGTTAACCGGAACAACCGAACAAGGGATCTCCCGACCTCAATCCACTGCACCCGCGATCCAAGATCCGAATGTTTACCTGCTTGTGGAGAACCAGATGTTTCGAGTTAGGCTCGAACTGCTTGCCGTCGACTATCAGGTGTTTTGTCGGCAAACTTGTGAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

20.66

Weight (kDa)

4.7

Isoelectric Point (pI)

64.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 468
AccI GTMKAC 1 cut(s) 510
AccII CGCG 4 cut(s) 55, 348, 380, 437
AccIII TCCGGA 1 cut(s) 271
AciI CCGC 3 cut(s) 55, 346, 435
AclWI GGATC 6 cut(s) 79, 269, 282, 416, 433, 440
AcsI RAATTY 1 cut(s) 203
AcuI CTGAAG 1 cut(s) 315
AfaI GTAC 1 cut(s) 363
AfiI CCNNNNNNNGG 3 cut(s) 389, 405, 466
AgsI TTSAA 2 cut(s) 79, 163
AhlI ACTAGT 1 cut(s) 358
AjiI CACGTC 1 cut(s) 51
AjnI CCWGG 1 cut(s) 242
AluBI AGCT 6 cut(s) 64, 111, 166, 289, 293, 299
AluI AGCT 6 cut(s) 64, 111, 166, 289, 293, 299
Alw21I GWGCWC 2 cut(s) 50, 66
Alw26I GTCTC 3 cut(s) 138, 149, 224
AlwI GGATC 6 cut(s) 79, 269, 282, 416, 433, 440
Aor13HI TCCGGA 1 cut(s) 271
ApeKI GCWGC 1 cut(s) 313
ApoI RAATTY 1 cut(s) 203
AspLEI GCGC 1 cut(s) 382
AspS9I GGNCC 1 cut(s) 22
AsuHPI GGTGA 1 cut(s) 199
AsuNHI GCTAGC 2 cut(s) 107, 289
AvaII GGWCC 1 cut(s) 22
BamHI GGATCC 1 cut(s) 274
BanII GRGCYC 1 cut(s) 66
Bbv12I GWGCWC 2 cut(s) 50, 66
BbvCI CCTCAGC 1 cut(s) 285
BbvI GCAGC 1 cut(s) 300
BceAI ACGGC 3 cut(s) 326, 367, 491
BcgI CGANNNNNNTGC 2 cut(s) 485, 519
BciT130I CCWGG 1 cut(s) 244
BcoDI GTCTC 3 cut(s) 138, 149, 224
BcuI ACTAGT 1 cut(s) 358
BfaI CTAG 3 cut(s) 108, 290, 359
BfuAI ACCTGC 1 cut(s) 468
BisI GCNGC 1 cut(s) 314
BlsI GCNGC 1 cut(s) 315
Bme1390I CCNGG 1 cut(s) 244
Bme18I GGWCC 1 cut(s) 22
BmgBI CACGTC 1 cut(s) 51
BmgT120I GGNCC 1 cut(s) 22
BmiI GGNNCC 2 cut(s) 241, 276
BmrFI CCNGG 1 cut(s) 244
BmtI GCTAGC 2 cut(s) 111, 293
BoxI GACNNNNGTC 1 cut(s) 20
BplI GAGNNNNNCTC 2 cut(s) 477, 509
BpmI CTGGAG 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 285
BpuEI CTTGAG 1 cut(s) 64
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 1 cut(s) 88
BsaWI WCCGGW 3 cut(s) 233, 271, 389
Bsc4I CCNNNNNNNGG 3 cut(s) 389, 405, 466
BseAI TCCGGA 1 cut(s) 271
BseBI CCWGG 1 cut(s) 244
BseDI CCNNGG 1 cut(s) 88
BseLI CCNNNNNNNGG 3 cut(s) 389, 405, 466
BseMII CTCAG 1 cut(s) 299
BseXI GCAGC 1 cut(s) 300
BsgI GTGCAG 2 cut(s) 299, 414
Bsh1236I CGCG 4 cut(s) 55, 348, 380, 437
Bsh1285I CGRYCG 2 cut(s) 72, 346
BsiEI CGRYCG 2 cut(s) 72, 346
BsiHKAI GWGCWC 2 cut(s) 50, 66
BsiSI CCGG 3 cut(s) 234, 272, 390
BslI CCNNNNNNNGG 3 cut(s) 389, 405, 466
BsmAI GTCTC 3 cut(s) 138, 149, 224
BsmBI CGTCTC 1 cut(s) 138
Bso31I GGTCTC 1 cut(s) 224
Bsp1286I GDGCHC 2 cut(s) 50, 66
Bsp13I TCCGGA 1 cut(s) 271
Bsp143I GATC 5 cut(s) 84, 274, 408, 438, 445
BspACI CCGC 3 cut(s) 55, 346, 435
BspCNI CTCAG 1 cut(s) 298
BspEI TCCGGA 1 cut(s) 271
BspFNI CGCG 4 cut(s) 55, 348, 380, 437
BspLI GGNNCC 2 cut(s) 241, 276
BspMI ACCTGC 1 cut(s) 468
BspOI GCTAGC 2 cut(s) 111, 293
BspPI GGATC 6 cut(s) 79, 269, 282, 416, 433, 440
BspTNI GGTCTC 1 cut(s) 224
BssECI CCNNGG 1 cut(s) 88
BssMI GATC 5 cut(s) 84, 274, 408, 438, 445
BssT1I CCWWGG 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 244
Bst4CI ACNGT 1 cut(s) 196
BstC8I GCNNGC 3 cut(s) 109, 291, 504
BstDEI CTNAG 1 cut(s) 285
BstFNI CGCG 4 cut(s) 55, 348, 380, 437
BstHHI GCGC 1 cut(s) 382
BstKTI GATC 5 cut(s) 87, 277, 411, 441, 448
BstMAI GTCTC 3 cut(s) 138, 149, 224
BstMBI GATC 5 cut(s) 84, 274, 408, 438, 445
BstMCI CGRYCG 2 cut(s) 72, 346
BstMWI GCNNNNNNNGC 2 cut(s) 61, 499
BstNI CCWGG 1 cut(s) 244
BstPAI GACNNNNGTC 1 cut(s) 20
BstSCI CCNGG 1 cut(s) 242
BstUI CGCG 4 cut(s) 55, 348, 380, 437
BstV1I GCAGC 1 cut(s) 300
BstX2I RGATCY 3 cut(s) 274, 408, 445
BstXI CCANNNNNNTGG 1 cut(s) 250
BstYI RGATCY 3 cut(s) 274, 408, 445
BtrI CACGTC 1 cut(s) 51
BtsI GCAGTG 1 cut(s) 426
BtsIMutI CAGTG 2 cut(s) 201, 426
BveI ACCTGC 1 cut(s) 468
Cac8I GCNNGC 3 cut(s) 109, 291, 504
CfoI GCGC 1 cut(s) 382
Cfr13I GGNCC 1 cut(s) 22
Csp6I GTAC 1 cut(s) 362
CviQI GTAC 1 cut(s) 362
DdeI CTNAG 1 cut(s) 285
DpnI GATC 5 cut(s) 86, 276, 410, 440, 447
DpnII GATC 5 cut(s) 84, 274, 408, 438, 445
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 88
Eco24I GRGCYC 1 cut(s) 66
Eco31I GGTCTC 1 cut(s) 224
Eco47I GGWCC 1 cut(s) 22
Eco53kI GAGCTC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 315
EcoICRI GAGCTC 1 cut(s) 64
EcoRI GAATTC 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 242
EcoT14I CCWWGG 1 cut(s) 88
EcoT38I GRGCYC 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 88
Esp3I CGTCTC 1 cut(s) 138
FaiI YATR 1 cut(s) 547
FalI AAGNNNNNCTT 2 cut(s) 252, 284
FauI CCCGC 1 cut(s) 442
FblI GTMKAC 1 cut(s) 510
Fnu4HI GCNGC 1 cut(s) 314
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 1 cut(s) 314
FspBI CTAG 3 cut(s) 108, 290, 359
GlaI GCGC 1 cut(s) 381
GluI GCNGC 1 cut(s) 314
GsuI CTGGAG 1 cut(s) 188
HapII CCGG 3 cut(s) 234, 272, 390
HhaI GCGC 1 cut(s) 382
Hin6I GCGC 1 cut(s) 380
HinP1I GCGC 1 cut(s) 380
HincII GTYRAC 3 cut(s) 15, 387, 511
HindII GTYRAC 3 cut(s) 15, 387, 511
HindIII AAGCTT 1 cut(s) 297
HinfI GANTC 4 cut(s) 16, 119, 212, 329
HpaI GTTAAC 1 cut(s) 387
HpaII CCGG 3 cut(s) 234, 272, 390
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 4 cut(s) 15, 387, 457, 511
Hpy188I TCNGA 2 cut(s) 115, 450
Hpy188III TCNNGA 5 cut(s) 25, 67, 272, 303, 414
Hpy8I GTNNAC 4 cut(s) 15, 387, 457, 511
Hpy99I CGWCG 3 cut(s) 55, 119, 512
HpyAV CCTTC 2 cut(s) 180, 254
HpyCH4III ACNGT 1 cut(s) 196
HpyCH4IV ACGT 1 cut(s) 50
HpyCH4V TGCA 2 cut(s) 316, 431
HpyF10VI GCNNNNNNNGC 2 cut(s) 61, 499
HpyF3I CTNAG 1 cut(s) 285
HpySE526I ACGT 1 cut(s) 50
HspAI GCGC 1 cut(s) 380
Kpn2I TCCGGA 1 cut(s) 271
KspAI GTTAAC 1 cut(s) 387
Kzo9I GATC 5 cut(s) 84, 274, 408, 438, 445
LmnI GCTCC 1 cut(s) 116
Lsp1109I GCAGC 1 cut(s) 300
MaeI CTAG 3 cut(s) 108, 290, 359
MaeII ACGT 1 cut(s) 50
MaeIII GTNAC 1 cut(s) 218
MalI GATC 5 cut(s) 86, 276, 410, 440, 447
MboI GATC 5 cut(s) 84, 274, 408, 438, 445
MboII GAAGA 2 cut(s) 195, 260
MflI RGATCY 3 cut(s) 274, 408, 445
MhlI GDGCHC 2 cut(s) 50, 66
MluCI AATT 2 cut(s) 150, 203
MlyI GAGTC 3 cut(s) 10, 128, 323
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 6 cut(s) 31, 96, 230, 294, 382, 429
MroI TCCGGA 1 cut(s) 271
MseI TTAA 1 cut(s) 386
MspI CCGG 3 cut(s) 234, 272, 390
MspR9I CCNGG 1 cut(s) 244
MvaI CCWGG 1 cut(s) 244
MvnI CGCG 4 cut(s) 55, 348, 380, 437
MwoI GCNNNNNNNGC 2 cut(s) 61, 499
NdeII GATC 5 cut(s) 84, 274, 408, 438, 445
NheI GCTAGC 2 cut(s) 107, 289
NlaIV GGNNCC 2 cut(s) 241, 276
NmeAIII GCCGAG 1 cut(s) 34
PcsI WCGNNNNNNNCGW 1 cut(s) 380
PfeI GAWTC 1 cut(s) 212
PkrI GCNGC 1 cut(s) 315
PleI GAGTC 3 cut(s) 10, 127, 323
PpsI GAGTC 3 cut(s) 10, 127, 323
PshAI GACNNNNGTC 1 cut(s) 20
Psp124BI GAGCTC 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 242
PspGI CCWGG 1 cut(s) 242
PspN4I GGNNCC 2 cut(s) 241, 276
PspPI GGNCC 1 cut(s) 22
PsuI RGATCY 3 cut(s) 274, 408, 445
RsaI GTAC 1 cut(s) 363
RsaNI GTAC 1 cut(s) 362
SacI GAGCTC 1 cut(s) 66
SalI GTCGAC 1 cut(s) 509
SaqAI TTAA 1 cut(s) 386
SatI GCNGC 1 cut(s) 314
Sau3AI GATC 5 cut(s) 84, 274, 408, 438, 445
Sau96I GGNCC 1 cut(s) 22
SchI GAGTC 3 cut(s) 10, 128, 323
ScrFI CCNGG 1 cut(s) 244
SduI GDGCHC 2 cut(s) 50, 66
SinI GGWCC 1 cut(s) 22
SmlI CTYRAG 1 cut(s) 43
SmoI CTYRAG 1 cut(s) 43
SpeI ACTAGT 1 cut(s) 358
Sse9I AATT 2 cut(s) 150, 203
SsiI CCGC 3 cut(s) 55, 346, 435
SspMI CTAG 3 cut(s) 108, 290, 359
SstI GAGCTC 1 cut(s) 66
StyD4I CCNGG 1 cut(s) 242
StyI CCWWGG 1 cut(s) 88
TaaI ACNGT 1 cut(s) 196
TaiI ACGT 1 cut(s) 53
TaqII GACCGA 1 cut(s) 86
TasI AATT 2 cut(s) 150, 203
TatI WGTACW 1 cut(s) 361
TfiI GAWTC 1 cut(s) 212
Tru1I TTAA 1 cut(s) 386
Tru9I TTAA 1 cut(s) 386
TscAI CASTG 2 cut(s) 201, 433
TseI GCWGC 1 cut(s) 313
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 201, 433
VpaK11BI GGWCC 1 cut(s) 22
XapI RAATTY 1 cut(s) 203
XmiI GTMKAC 1 cut(s) 510
XspI CTAG 3 cut(s) 108, 290, 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.