Rw5G043420

Protein FLC EXPRESSOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000946
Physical Location & Seq
Reverse (-)
76260230 .. 76261993
1764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G043420.1

Sequence Viewer

Length: 753 bp
ATGGCCGGAAGAGAGCCTCGGCATCTCCACCGCCTTCCCCAAATCGTCAACACGACGGCCCTCGAGGACCGCATCGATCTCCAGCAACGCGAGATCCAGTCCCTCCTCGGGGAGAATCAACGCCTCGCGGCCACCCACGTGGCGCTCAAGCAGGACCTAACCGCCGCTCAGAGCGACCTCCGCAACCTGAAGGCCGTCGCCGGCCAAATCAAATCCGAGAGGAACGCCGAAGTCCGCGAGGTCTACGACAGGTCGTTGAAGCTGGACGACGAGCTCCGCGACCTGGACGCCATGAACGCCGAGTTGACTCTGGTCCGGAACGACATCGAGGACTTGAGCACGTCGCGGATGGAGCTCGCGAACGAGTTGAAGACGATCCAAGGAGAGATTGAGAGGGCTCGGTCCGACGAGTCGAAGCAAATCGAAGCCATTAGAGACGAAATTGAGACCTTGAAGCTGGAGATTGAGAAAGGAAGTACTGCGGTTGAGATTGAGAAGAAAACACGAGCTAGTAACTTTGAGCATCGTCAAGCTATGGAGAATTATATGGCTAAGTTGGCTCTCGAAATCGAAAAGCTTCATGGGGAGTTGGCAAATGCAGAGAAGAGAGCAAGGGCTGCAGTAGCAGCAGCAAATCCAGGTTGTTGTTTGGTTGCAGGTTCTGGGTACCCTATGGCTTATGGCAATGCCGAAATGCTATATGGAGGAAATGCATACCCTGATCCCTATGCCATGCATCAGGTATGTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

250

Amino Acids

27.97

Weight (kDa)

5.24

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 62
Acc36I ACCTGC 1 cut(s) 647
Acc65I GGTACC 1 cut(s) 666
AccB1I GGYRCC 1 cut(s) 666
AccBSI CCGCTC 1 cut(s) 167
AccI GTMKAC 1 cut(s) 243
AccII CGCG 6 cut(s) 90, 128, 237, 279, 346, 359
AccIII TCCGGA 1 cut(s) 315
AclWI GGATC 3 cut(s) 88, 370, 716
AcoI YGGCCR 3 cut(s) 3, 129, 202
AcuI CTGAAG 1 cut(s) 209
AcvI CACGTG 1 cut(s) 139
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 2 cut(s) 478, 668
AfiI CCNNNNNNNGG 3 cut(s) 108, 109, 283
AgsI TTSAA 3 cut(s) 259, 370, 454
AjiI CACGTC 1 cut(s) 342
AjnI CCWGG 2 cut(s) 282, 637
AleI CACNNNNGTG 1 cut(s) 137
AluBI AGCT 7 cut(s) 262, 274, 355, 457, 509, 533, 577
AluI AGCT 7 cut(s) 262, 274, 355, 457, 509, 533, 577
Alw21I GWGCWC 3 cut(s) 276, 341, 357
Alw26I GTCTC 2 cut(s) 429, 440
AlwI GGATC 3 cut(s) 88, 370, 716
AlwNI CAGNNNCTG 1 cut(s) 662
Ama87I CYCGRG 2 cut(s) 62, 107
Aor13HI TCCGGA 1 cut(s) 315
AoxI GGCC 5 cut(s) 3, 57, 129, 192, 202
ApeKI GCWGC 3 cut(s) 617, 626, 629
Asp700I GAANNNNTTC 1 cut(s) 576
Asp718I GGTACC 1 cut(s) 666
AspLEI GCGC 1 cut(s) 145
AspS9I GGNCC 5 cut(s) 58, 67, 154, 313, 402
AvaI CYCGRG 2 cut(s) 62, 107
AvaII GGWCC 4 cut(s) 67, 154, 313, 402
BanI GGYRCC 1 cut(s) 666
BanII GRGCYC 3 cut(s) 276, 357, 400
BauI CACGAG 1 cut(s) 504
BbrPI CACGTG 1 cut(s) 139
BbsI GAAGAC 1 cut(s) 377
Bbv12I GWGCWC 3 cut(s) 276, 341, 357
BbvI GCAGC 3 cut(s) 604, 638, 641
BccI CCATC 1 cut(s) 343
BceAI ACGGC 2 cut(s) 72, 179
BciT130I CCWGG 2 cut(s) 284, 639
BcoDI GTCTC 2 cut(s) 429, 440
BfaI CTAG 1 cut(s) 510
BfmI CTRYAG 1 cut(s) 618
BfoI RGCGCY 1 cut(s) 146
BfuAI ACCTGC 1 cut(s) 647
BisI GCNGC 5 cut(s) 129, 165, 618, 627, 630
BlsI GCNGC 5 cut(s) 130, 166, 619, 628, 631
BmcAI AGTACT 1 cut(s) 478
Bme1390I CCNGG 2 cut(s) 284, 639
Bme18I GGWCC 4 cut(s) 67, 154, 313, 402
BmeT110I CYCGRG 2 cut(s) 62, 107
BmgBI CACGTC 1 cut(s) 342
BmgT120I GGNCC 5 cut(s) 58, 67, 154, 313, 402
BmiI GGNNCC 1 cut(s) 668
BmrFI CCNGG 2 cut(s) 284, 639
BmsI GCATC 4 cut(s) 31, 81, 532, 745
BoxI GACNNNNGTC 1 cut(s) 311
BpiI GAAGAC 1 cut(s) 377
BpmI CTGGAG 2 cut(s) 65, 479
BpuEI CTTGAG 2 cut(s) 131, 355
Bsa29I ATCGAT 1 cut(s) 75
BsaAI YACGTR 1 cut(s) 139
BsaHI GRCGYC 1 cut(s) 288
BsaI GGTCTC 1 cut(s) 440
BsaJI CCNNGG 3 cut(s) 17, 106, 379
BsaWI WCCGGW 1 cut(s) 315
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 109, 283
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 97
Bse3DI GCAATG 1 cut(s) 691
BseAI TCCGGA 1 cut(s) 315
BseBI CCWGG 2 cut(s) 284, 639
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 3 cut(s) 17, 106, 379
BseGI GGATG 1 cut(s) 354
BseLI CCNNNNNNNGG 3 cut(s) 108, 109, 283
BseMI GCAATG 1 cut(s) 691
BseMII CTCAG 1 cut(s) 182
BseNI ACTGG 1 cut(s) 97
BseRI GAGGAG 1 cut(s) 95
BseXI GCAGC 3 cut(s) 604, 638, 641
Bsh1236I CGCG 6 cut(s) 90, 128, 237, 279, 346, 359
BshFI GGCC 5 cut(s) 5, 59, 131, 194, 204
BshNI GGYRCC 1 cut(s) 666
BshVI ATCGAT 1 cut(s) 75
BsiHKAI GWGCWC 3 cut(s) 276, 341, 357
BsiHKCI CYCGRG 2 cut(s) 62, 107
BsiSI CCGG 3 cut(s) 6, 201, 316
BslFI GGGAC 1 cut(s) 85
BslI CCNNNNNNNGG 3 cut(s) 108, 109, 283
BsmAI GTCTC 2 cut(s) 429, 440
BsmBI CGTCTC 1 cut(s) 429
BsmFI GGGAC 1 cut(s) 85
BsnI GGCC 5 cut(s) 5, 59, 131, 194, 204
Bso31I GGTCTC 1 cut(s) 440
BsoBI CYCGRG 2 cut(s) 62, 107
Bsp1286I GDGCHC 4 cut(s) 276, 341, 357, 400
Bsp13I TCCGGA 1 cut(s) 315
Bsp143I GATC 4 cut(s) 76, 93, 375, 721
Bsp68I TCGCGA 1 cut(s) 359
BspANI GGCC 5 cut(s) 5, 59, 131, 194, 204
BspCNI CTCAG 1 cut(s) 181
BspDI ATCGAT 1 cut(s) 75
BspEI TCCGGA 1 cut(s) 315
BspFNI CGCG 6 cut(s) 90, 128, 237, 279, 346, 359
BspLI GGNNCC 1 cut(s) 668
BspMAI CTGCAG 1 cut(s) 622
BspMI ACCTGC 1 cut(s) 647
BspPI GGATC 3 cut(s) 88, 370, 716
BspT107I GGYRCC 1 cut(s) 666
BspTNI GGTCTC 1 cut(s) 440
BsrBI CCGCTC 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 691
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 1 cut(s) 97
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 3 cut(s) 17, 106, 379
BssMI GATC 4 cut(s) 76, 93, 375, 721
BssNI GRCGYC 1 cut(s) 288
BssSI CACGAG 1 cut(s) 504
BssT1I CCWWGG 1 cut(s) 379
Bst2BI CACGAG 1 cut(s) 504
Bst2UI CCWGG 2 cut(s) 284, 639
Bst6I CTCTTC 2 cut(s) 4, 599
BstACI GRCGYC 1 cut(s) 288
BstAPI GCANNNNNTGC 1 cut(s) 617
BstBAI YACGTR 1 cut(s) 139
BstC8I GCNNGC 2 cut(s) 202, 357
BstDEI CTNAG 2 cut(s) 168, 552
BstF5I GGATG 1 cut(s) 354
BstFNI CGCG 6 cut(s) 90, 128, 237, 279, 346, 359
BstH2I RGCGCY 1 cut(s) 146
BstHHI GCGC 1 cut(s) 145
BstKTI GATC 4 cut(s) 79, 96, 378, 724
BstMAI GTCTC 2 cut(s) 429, 440
BstMBI GATC 4 cut(s) 76, 93, 375, 721
BstMWI GCNNNNNNNGC 7 cut(s) 180, 296, 352, 557, 617, 623, 626
BstNI CCWGG 2 cut(s) 284, 639
BstPAI GACNNNNGTC 1 cut(s) 311
BstSCI CCNGG 2 cut(s) 282, 637
BstSFI CTRYAG 1 cut(s) 618
BstUI CGCG 6 cut(s) 90, 128, 237, 279, 346, 359
BstV1I GCAGC 3 cut(s) 604, 638, 641
BstV2I GAAGAC 1 cut(s) 377
BstX2I RGATCY 1 cut(s) 93
BstXI CCANNNNNNTGG 1 cut(s) 139
BstYI RGATCY 1 cut(s) 93
Bsu15I ATCGAT 1 cut(s) 75
BsuRI GGCC 5 cut(s) 5, 59, 131, 194, 204
BsuTUI ATCGAT 1 cut(s) 75
BtrI CACGTC 1 cut(s) 342
BtsCI GGATG 1 cut(s) 354
BtuMI TCGCGA 1 cut(s) 359
BveI ACCTGC 1 cut(s) 647
Cac8I GCNNGC 2 cut(s) 202, 357
CaiI CAGNNNCTG 1 cut(s) 662
CfoI GCGC 1 cut(s) 145
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 5 cut(s) 58, 67, 154, 313, 402
ClaI ATCGAT 1 cut(s) 75
CpoI CGGWCCG 1 cut(s) 402
CseI GACGC 1 cut(s) 296
Csp6I GTAC 2 cut(s) 477, 667
CspI CGGWCCG 1 cut(s) 402
CviAII CATG 3 cut(s) 292, 581, 733
CviQI GTAC 2 cut(s) 477, 667
DdeI CTNAG 2 cut(s) 168, 552
DpnI GATC 4 cut(s) 78, 95, 377, 723
DpnII GATC 4 cut(s) 76, 93, 375, 721
EaeI YGGCCR 3 cut(s) 3, 129, 202
Eam1104I CTCTTC 2 cut(s) 4, 599
EarI CTCTTC 2 cut(s) 4, 599
Ecl136II GAGCTC 2 cut(s) 274, 355
Eco130I CCWWGG 1 cut(s) 379
Eco24I GRGCYC 3 cut(s) 276, 357, 400
Eco31I GGTCTC 1 cut(s) 440
Eco47I GGWCC 4 cut(s) 67, 154, 313, 402
Eco53kI GAGCTC 2 cut(s) 274, 355
Eco57I CTGAAG 1 cut(s) 209
Eco72I CACGTG 1 cut(s) 139
Eco88I CYCGRG 2 cut(s) 62, 107
EcoICRI GAGCTC 2 cut(s) 274, 355
EcoO109I RGGNCCY 1 cut(s) 154
EcoRII CCWGG 2 cut(s) 282, 637
EcoT14I CCWWGG 1 cut(s) 379
EcoT22I ATGCAT 2 cut(s) 715, 738
EcoT38I GRGCYC 3 cut(s) 276, 357, 400
ErhI CCWWGG 1 cut(s) 379
Esp3I CGTCTC 1 cut(s) 429
FaeI CATG 3 cut(s) 295, 584, 736
FaqI GGGAC 1 cut(s) 85
FatI CATG 3 cut(s) 291, 580, 732
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 5 cut(s) 129, 165, 618, 627, 630
FokI GGATG 1 cut(s) 361
FriOI GRGCYC 3 cut(s) 276, 357, 400
Fsp4HI GCNGC 5 cut(s) 129, 165, 618, 627, 630
FspBI CTAG 1 cut(s) 510
GlaI GCGC 1 cut(s) 144
GluI GCNGC 5 cut(s) 129, 165, 618, 627, 630
GsuI CTGGAG 2 cut(s) 65, 479
HaeII RGCGCY 1 cut(s) 146
HaeIII GGCC 5 cut(s) 5, 59, 131, 194, 204
HapII CCGG 3 cut(s) 6, 201, 316
HgaI GACGC 1 cut(s) 296
HhaI GCGC 1 cut(s) 145
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 3 cut(s) 295, 584, 736
Hin6I GCGC 1 cut(s) 143
HinP1I GCGC 1 cut(s) 143
HincII GTYRAC 2 cut(s) 49, 306
HindII GTYRAC 2 cut(s) 49, 306
HindIII AAGCTT 1 cut(s) 575
HinfI GANTC 3 cut(s) 115, 307, 410
HpaII CCGG 3 cut(s) 6, 201, 316
Hpy166II GTNNAC 3 cut(s) 49, 244, 306
Hpy188I TCNGA 3 cut(s) 171, 217, 406
Hpy188III TCNNGA 3 cut(s) 316, 358, 563
Hpy8I GTNNAC 3 cut(s) 49, 244, 306
Hpy99I CGWCG 5 cut(s) 58, 200, 272, 346, 410
HpyAV CCTTC 2 cut(s) 44, 184
HpyCH4IV ACGT 2 cut(s) 138, 341
HpyCH4V TGCA 5 cut(s) 599, 620, 656, 713, 736
HpyF10VI GCNNNNNNNGC 7 cut(s) 180, 296, 352, 557, 617, 623, 626
HpyF3I CTNAG 2 cut(s) 168, 552
HpySE526I ACGT 2 cut(s) 138, 341
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 3 cut(s) 295, 584, 736
HspAI GCGC 1 cut(s) 143
Kpn2I TCCGGA 1 cut(s) 315
KpnI GGTACC 1 cut(s) 670
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Kzo9I GATC 4 cut(s) 76, 93, 375, 721
LmnI GCTCC 2 cut(s) 279, 352
Lsp1109I GCAGC 3 cut(s) 604, 638, 641
LweI GCATC 4 cut(s) 31, 81, 532, 745
MaeI CTAG 1 cut(s) 510
MaeII ACGT 2 cut(s) 138, 341
MaeIII GTNAC 1 cut(s) 512
MalI GATC 4 cut(s) 78, 95, 377, 723
MbiI CCGCTC 1 cut(s) 167
MboI GATC 4 cut(s) 76, 93, 375, 721
MboII GAAGA 4 cut(s) 21, 382, 508, 616
MflI RGATCY 1 cut(s) 93
MhlI GDGCHC 4 cut(s) 276, 341, 357, 400
MluCI AATT 2 cut(s) 441, 541
MlyI GAGTC 2 cut(s) 301, 419
MmeI TCCRAC 1 cut(s) 429
Mph1103I ATGCAT 2 cut(s) 715, 738
MroI TCCGGA 1 cut(s) 315
MroNI GCCGGC 1 cut(s) 200
MroXI GAANNNNTTC 1 cut(s) 576
MslI CAYNNNNRTG 1 cut(s) 137
MspI CCGG 3 cut(s) 6, 201, 316
MspR9I CCNGG 2 cut(s) 284, 639
MvaI CCWGG 2 cut(s) 284, 639
MvnI CGCG 6 cut(s) 90, 128, 237, 279, 346, 359
MwoI GCNNNNNNNGC 7 cut(s) 180, 296, 352, 557, 617, 623, 626
NaeI GCCGGC 1 cut(s) 202
NdeII GATC 4 cut(s) 76, 93, 375, 721
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 3 cut(s) 295, 584, 736
NlaIV GGNNCC 1 cut(s) 668
NmeAIII GCCGAG 1 cut(s) 325
NruI TCGCGA 1 cut(s) 359
NsiI ATGCAT 2 cut(s) 715, 738
OliI CACNNNNGTG 1 cut(s) 137
PaeR7I CTCGAG 1 cut(s) 62
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PdiI GCCGGC 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 576
PfeI GAWTC 1 cut(s) 115
PkrI GCNGC 5 cut(s) 130, 166, 619, 628, 631
PleI GAGTC 2 cut(s) 301, 418
PmaCI CACGTG 1 cut(s) 139
PmlI CACGTG 1 cut(s) 139
PpsI GAGTC 2 cut(s) 301, 418
Ppu21I YACGTR 1 cut(s) 139
PpuMI RGGWCCY 1 cut(s) 154
PshAI GACNNNNGTC 1 cut(s) 311
Psp124BI GAGCTC 2 cut(s) 276, 357
Psp5II RGGWCCY 1 cut(s) 154
Psp6I CCWGG 2 cut(s) 282, 637
PspCI CACGTG 1 cut(s) 139
PspGI CCWGG 2 cut(s) 282, 637
PspN4I GGNNCC 1 cut(s) 668
PspPI GGNCC 5 cut(s) 58, 67, 154, 313, 402
PspPPI RGGWCCY 1 cut(s) 154
PspXI VCTCGAGB 1 cut(s) 62
PstI CTGCAG 1 cut(s) 622
PstNI CAGNNNCTG 1 cut(s) 662
PsuI RGATCY 1 cut(s) 93
RruI TCGCGA 1 cut(s) 359
RsaI GTAC 2 cut(s) 478, 668
RsaNI GTAC 2 cut(s) 477, 667
RseI CAYNNNNRTG 1 cut(s) 137
Rsr2I CGGWCCG 1 cut(s) 402
RsrII CGGWCCG 1 cut(s) 402
SacI GAGCTC 2 cut(s) 276, 357
SatI GCNGC 5 cut(s) 129, 165, 618, 627, 630
Sau3AI GATC 4 cut(s) 76, 93, 375, 721
Sau96I GGNCC 5 cut(s) 58, 67, 154, 313, 402
ScaI AGTACT 1 cut(s) 478
SchI GAGTC 2 cut(s) 301, 419
ScrFI CCNGG 2 cut(s) 284, 639
SduI GDGCHC 4 cut(s) 276, 341, 357, 400
SfaNI GCATC 4 cut(s) 31, 81, 532, 745
SfcI CTRYAG 1 cut(s) 618
Sfr274I CTCGAG 1 cut(s) 62
SinI GGWCC 4 cut(s) 67, 154, 313, 402
SlaI CTCGAG 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 3 cut(s) 62, 146, 334
SmoI CTYRAG 3 cut(s) 62, 146, 334
Sse9I AATT 2 cut(s) 441, 541
SspMI CTAG 1 cut(s) 510
SstI GAGCTC 2 cut(s) 276, 357
StyD4I CCNGG 2 cut(s) 282, 637
StyI CCWWGG 1 cut(s) 379
TaiI ACGT 2 cut(s) 141, 344
TaqI TCGA 7 cut(s) 63, 75, 327, 413, 423, 564, 570
TaqII GACCGA 1 cut(s) 390
TasI AATT 2 cut(s) 441, 541
TatI WGTACW 1 cut(s) 476
TauI GCSGC 2 cut(s) 131, 167
TfiI GAWTC 1 cut(s) 115
TseI GCWGC 3 cut(s) 617, 626, 629
TspDTI ATGAA 2 cut(s) 308, 569
VpaK11BI GGWCC 4 cut(s) 67, 154, 313, 402
XhoI CTCGAG 1 cut(s) 62
XmiI GTMKAC 1 cut(s) 243
XmnI GAANNNNTTC 1 cut(s) 576
XspI CTAG 1 cut(s) 510
ZrmI AGTACT 1 cut(s) 478
Zsp2I ATGCAT 2 cut(s) 715, 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.