RLG00000036199

Protein FLC EXPRESSOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000946
Physical Location & Seq
Reverse (-)
77373553 .. 77375360
1808 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036199

Sequence Viewer

Length: 816 bp
ATGGCCGGAAGAGAGCCTCGGCATCTCCACCGCCTTCCCCAAATCGTCAACACGACGGCACTCGAGGACCGCATCGATCTCCAGCAACGCGAGATCCAGTCCCTCCTCGGGGACAATCAACGCCTCGCGGCCACCCACGTGGCGCTCAAGCAGGACATAACCGCCGCTCAGAGCGACCTCCGCAACCTGAAGGCCGTCGCCGGCCAAATCAAATCCGAGAGGAACGCCGAAGTCCGTGAGGTCTACGACAGGTCGTTGAAGCTGGACGACGAGCTCCGCGACCTGGGCGCCATGAACGACGAGTTGACTCTGGTCCGGAACGACATCGAGGACTTGAGCACGTCGCGGATGGAACTCGCGACCGAGTTGAAGACGATCCAAGGAGAGATTGAGAGGGCTCGGTCCGACGAGTCGAAGCAAATCGAAGCCATTAGAGACGAAATTGAGACTTTGAAGCTGGAGATTGAGAAAGGAAGGACTGCGGTTGAGATTGAGAAGAAAACACGAGCTAGTAACCTTGAGCATCGTCAAGCTATGGAGAATTATATGGCTAAGTTGGCTCTCGAAATCGAAAAGCTTCATGGGGAGTTGGCAAATGCAGAGAAGAGAGCAAGGGCTGCAGTAGCAGCTGCAAATCCAGGTTCTGGGTACCCTATGGCTTATGGCAATGCCGACATGCTATATGGAGGAAAGGCATACCCTGACTCCTATGCCATGCATCAGGGTCAGGGCAGTGCTGATGGTACCCCTCAATTTGGCTCCGCGCAAATGCCACATAGTTCCTATGATATTCAACAAACACATGTACATAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

272

Amino Acids

30.31

Weight (kDa)

5.56

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 648, 743
AccB1I GGYRCC 3 cut(s) 287, 648, 743
AccB7I CCANNNNNTGG 1 cut(s) 644
AccBSI CCGCTC 1 cut(s) 167
AccI GTMKAC 1 cut(s) 243
AccII CGCG 6 cut(s) 90, 128, 279, 346, 359, 764
AccIII TCCGGA 1 cut(s) 315
AclWI GGATC 2 cut(s) 88, 370
AcoI YGGCCR 3 cut(s) 3, 129, 202
AcuI CTGAAG 1 cut(s) 209
AcvI CACGTG 1 cut(s) 139
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 3 cut(s) 650, 745, 807
AfiI CCNNNNNNNGG 5 cut(s) 108, 109, 283, 644, 755
AflIII ACRYGT 1 cut(s) 802
AgsI TTSAA 4 cut(s) 259, 370, 454, 794
AjiI CACGTC 1 cut(s) 342
AjnI CCWGG 2 cut(s) 282, 637
AleI CACNNNNGTG 1 cut(s) 137
AluBI AGCT 7 cut(s) 262, 274, 457, 509, 533, 577, 629
AluI AGCT 7 cut(s) 262, 274, 457, 509, 533, 577, 629
Alw21I GWGCWC 2 cut(s) 276, 341
Alw26I GTCTC 2 cut(s) 429, 440
AlwI GGATC 2 cut(s) 88, 370
AlwNI CAGNNNCTG 1 cut(s) 644
Ama87I CYCGRG 2 cut(s) 62, 107
Aor13HI TCCGGA 1 cut(s) 315
AoxI GGCC 4 cut(s) 3, 129, 192, 202
ApeKI GCWGC 3 cut(s) 617, 626, 629
Asp700I GAANNNNTTC 1 cut(s) 576
Asp718I GGTACC 2 cut(s) 648, 743
AspLEI GCGC 3 cut(s) 145, 290, 766
AspS9I GGNCC 3 cut(s) 67, 313, 402
AvaI CYCGRG 2 cut(s) 62, 107
AvaII GGWCC 3 cut(s) 67, 313, 402
BanI GGYRCC 3 cut(s) 287, 648, 743
BanII GRGCYC 2 cut(s) 276, 400
BauI CACGAG 1 cut(s) 504
BbrPI CACGTG 1 cut(s) 139
BbsI GAAGAC 1 cut(s) 377
Bbv12I GWGCWC 2 cut(s) 276, 341
BbvI GCAGC 3 cut(s) 604, 616, 638
BccI CCATC 2 cut(s) 343, 734
BceAI ACGGC 2 cut(s) 72, 179
BciT130I CCWGG 2 cut(s) 284, 639
BcoDI GTCTC 2 cut(s) 429, 440
BfaI CTAG 1 cut(s) 510
BfmI CTRYAG 1 cut(s) 618
BfoI RGCGCY 2 cut(s) 146, 291
BisI GCNGC 5 cut(s) 129, 165, 618, 627, 630
BlsI GCNGC 5 cut(s) 130, 166, 619, 628, 631
Bme1390I CCNGG 2 cut(s) 284, 639
Bme18I GGWCC 3 cut(s) 67, 313, 402
BmeT110I CYCGRG 2 cut(s) 62, 107
BmgBI CACGTC 1 cut(s) 342
BmgT120I GGNCC 3 cut(s) 67, 313, 402
BmiI GGNNCC 4 cut(s) 289, 650, 745, 760
BmrFI CCNGG 2 cut(s) 284, 639
BmsI GCATC 4 cut(s) 31, 81, 532, 727
BoxI GACNNNNGTC 1 cut(s) 311
BpiI GAAGAC 1 cut(s) 377
BpmI CTGGAG 2 cut(s) 65, 479
BpuEI CTTGAG 3 cut(s) 131, 355, 539
Bsa29I ATCGAT 1 cut(s) 75
BsaAI YACGTR 1 cut(s) 139
BsaHI GRCGYC 1 cut(s) 288
BsaJI CCNNGG 4 cut(s) 17, 106, 283, 379
BsaWI WCCGGW 1 cut(s) 315
BsaXI ACNNNNNCTCC 2 cut(s) 689, 719
Bsc4I CCNNNNNNNGG 5 cut(s) 108, 109, 283, 644, 755
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 97
Bse3DI GCAATG 1 cut(s) 673
BseAI TCCGGA 1 cut(s) 315
BseBI CCWGG 2 cut(s) 284, 639
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 4 cut(s) 17, 106, 283, 379
BseGI GGATG 1 cut(s) 354
BseLI CCNNNNNNNGG 5 cut(s) 108, 109, 283, 644, 755
BseMI GCAATG 1 cut(s) 673
BseMII CTCAG 1 cut(s) 182
BseNI ACTGG 1 cut(s) 97
BseRI GAGGAG 1 cut(s) 95
BseXI GCAGC 3 cut(s) 604, 616, 638
Bsh1236I CGCG 6 cut(s) 90, 128, 279, 346, 359, 764
Bsh1285I CGRYCG 1 cut(s) 363
BshFI GGCC 4 cut(s) 5, 131, 194, 204
BshNI GGYRCC 3 cut(s) 287, 648, 743
BshVI ATCGAT 1 cut(s) 75
BsiEI CGRYCG 1 cut(s) 363
BsiHKAI GWGCWC 2 cut(s) 276, 341
BsiHKCI CYCGRG 2 cut(s) 62, 107
BsiSI CCGG 3 cut(s) 6, 201, 316
BslFI GGGAC 2 cut(s) 85, 125
BslI CCNNNNNNNGG 5 cut(s) 108, 109, 283, 644, 755
BsmAI GTCTC 2 cut(s) 429, 440
BsmBI CGTCTC 1 cut(s) 429
BsmFI GGGAC 2 cut(s) 85, 125
BsnI GGCC 4 cut(s) 5, 131, 194, 204
BsoBI CYCGRG 2 cut(s) 62, 107
Bsp1286I GDGCHC 3 cut(s) 276, 341, 400
Bsp13I TCCGGA 1 cut(s) 315
Bsp1407I TGTACA 1 cut(s) 805
Bsp143I GATC 3 cut(s) 76, 93, 375
Bsp68I TCGCGA 1 cut(s) 359
BspANI GGCC 4 cut(s) 5, 131, 194, 204
BspCNI CTCAG 1 cut(s) 181
BspDI ATCGAT 1 cut(s) 75
BspEI TCCGGA 1 cut(s) 315
BspFNI CGCG 6 cut(s) 90, 128, 279, 346, 359, 764
BspLI GGNNCC 4 cut(s) 289, 650, 745, 760
BspMAI CTGCAG 1 cut(s) 622
BspPI GGATC 2 cut(s) 88, 370
BspT107I GGYRCC 3 cut(s) 287, 648, 743
BsrBI CCGCTC 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 673
BsrFI RCCGGY 1 cut(s) 200
BsrGI TGTACA 1 cut(s) 805
BsrI ACTGG 1 cut(s) 97
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 4 cut(s) 17, 106, 283, 379
BssMI GATC 3 cut(s) 76, 93, 375
BssNI GRCGYC 1 cut(s) 288
BssSI CACGAG 1 cut(s) 504
BssT1I CCWWGG 1 cut(s) 379
Bst2BI CACGAG 1 cut(s) 504
Bst2UI CCWGG 2 cut(s) 284, 639
Bst6I CTCTTC 2 cut(s) 4, 599
BstACI GRCGYC 1 cut(s) 288
BstAPI GCANNNNNTGC 1 cut(s) 617
BstAUI TGTACA 1 cut(s) 805
BstBAI YACGTR 1 cut(s) 139
BstC8I GCNNGC 1 cut(s) 202
BstDEI CTNAG 2 cut(s) 168, 552
BstF5I GGATG 1 cut(s) 354
BstFNI CGCG 6 cut(s) 90, 128, 279, 346, 359, 764
BstH2I RGCGCY 2 cut(s) 146, 291
BstHHI GCGC 3 cut(s) 145, 290, 766
BstKTI GATC 3 cut(s) 79, 96, 378
BstMAI GTCTC 2 cut(s) 429, 440
BstMBI GATC 3 cut(s) 76, 93, 375
BstMCI CGRYCG 1 cut(s) 363
BstMWI GCNNNNNNNGC 6 cut(s) 180, 285, 557, 617, 623, 626
BstNI CCWGG 2 cut(s) 284, 639
BstNSI RCATGY 2 cut(s) 679, 806
BstPAI GACNNNNGTC 1 cut(s) 311
BstSCI CCNGG 2 cut(s) 282, 637
BstSFI CTRYAG 1 cut(s) 618
BstUI CGCG 6 cut(s) 90, 128, 279, 346, 359, 764
BstV1I GCAGC 3 cut(s) 604, 616, 638
BstV2I GAAGAC 1 cut(s) 377
BstX2I RGATCY 1 cut(s) 93
BstXI CCANNNNNNTGG 1 cut(s) 139
BstYI RGATCY 1 cut(s) 93
Bsu15I ATCGAT 1 cut(s) 75
BsuRI GGCC 4 cut(s) 5, 131, 194, 204
BsuTUI ATCGAT 1 cut(s) 75
BtrI CACGTC 1 cut(s) 342
BtsCI GGATG 1 cut(s) 354
BtsI GCAGTG 1 cut(s) 739
BtsIMutI CAGTG 1 cut(s) 739
BtuMI TCGCGA 1 cut(s) 359
Cac8I GCNNGC 1 cut(s) 202
CaiI CAGNNNCTG 1 cut(s) 644
CfoI GCGC 3 cut(s) 145, 290, 766
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 3 cut(s) 67, 313, 402
ClaI ATCGAT 1 cut(s) 75
CpoI CGGWCCG 1 cut(s) 402
Csp6I GTAC 3 cut(s) 649, 744, 806
CspI CGGWCCG 1 cut(s) 402
CviAII CATG 5 cut(s) 292, 581, 676, 715, 803
CviQI GTAC 3 cut(s) 649, 744, 806
DdeI CTNAG 2 cut(s) 168, 552
DinI GGCGCC 1 cut(s) 289
DpnI GATC 3 cut(s) 78, 95, 377
DpnII GATC 3 cut(s) 76, 93, 375
EaeI YGGCCR 3 cut(s) 3, 129, 202
Eam1104I CTCTTC 2 cut(s) 4, 599
EarI CTCTTC 2 cut(s) 4, 599
Ecl136II GAGCTC 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 379
Eco24I GRGCYC 2 cut(s) 276, 400
Eco47I GGWCC 3 cut(s) 67, 313, 402
Eco53kI GAGCTC 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 209
Eco72I CACGTG 1 cut(s) 139
Eco88I CYCGRG 2 cut(s) 62, 107
EcoICRI GAGCTC 1 cut(s) 274
EcoRII CCWGG 2 cut(s) 282, 637
EcoT14I CCWWGG 1 cut(s) 379
EcoT22I ATGCAT 1 cut(s) 720
EcoT38I GRGCYC 2 cut(s) 276, 400
EgeI GGCGCC 1 cut(s) 289
EheI GGCGCC 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 379
Esp3I CGTCTC 1 cut(s) 429
FaeI CATG 5 cut(s) 295, 584, 679, 718, 806
FaqI GGGAC 2 cut(s) 85, 125
FatI CATG 5 cut(s) 291, 580, 675, 714, 802
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 5 cut(s) 129, 165, 618, 627, 630
FokI GGATG 1 cut(s) 361
FriOI GRGCYC 2 cut(s) 276, 400
Fsp4HI GCNGC 5 cut(s) 129, 165, 618, 627, 630
FspBI CTAG 1 cut(s) 510
GlaI GCGC 3 cut(s) 144, 289, 765
GluI GCNGC 5 cut(s) 129, 165, 618, 627, 630
GsuI CTGGAG 2 cut(s) 65, 479
HaeII RGCGCY 2 cut(s) 146, 291
HaeIII GGCC 4 cut(s) 5, 131, 194, 204
HapII CCGG 3 cut(s) 6, 201, 316
HhaI GCGC 3 cut(s) 145, 290, 766
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 5 cut(s) 295, 584, 679, 718, 806
Hin6I GCGC 3 cut(s) 143, 288, 764
HinP1I GCGC 3 cut(s) 143, 288, 764
HincII GTYRAC 2 cut(s) 49, 306
HindII GTYRAC 2 cut(s) 49, 306
HindIII AAGCTT 1 cut(s) 575
HinfI GANTC 3 cut(s) 307, 410, 704
HpaII CCGG 3 cut(s) 6, 201, 316
Hpy166II GTNNAC 3 cut(s) 49, 244, 306
Hpy188I TCNGA 3 cut(s) 171, 217, 406
Hpy188III TCNNGA 3 cut(s) 316, 358, 563
Hpy8I GTNNAC 3 cut(s) 49, 244, 306
Hpy99I CGWCG 6 cut(s) 58, 200, 272, 302, 346, 410
HpyAV CCTTC 3 cut(s) 44, 184, 468
HpyCH4IV ACGT 2 cut(s) 138, 341
HpyCH4V TGCA 4 cut(s) 599, 620, 632, 718
HpyF10VI GCNNNNNNNGC 6 cut(s) 180, 285, 557, 617, 623, 626
HpyF3I CTNAG 2 cut(s) 168, 552
HpySE526I ACGT 2 cut(s) 138, 341
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 5 cut(s) 295, 584, 679, 718, 806
HspAI GCGC 3 cut(s) 143, 288, 764
KasI GGCGCC 1 cut(s) 287
Kpn2I TCCGGA 1 cut(s) 315
KpnI GGTACC 2 cut(s) 652, 747
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Kzo9I GATC 3 cut(s) 76, 93, 375
LmnI GCTCC 2 cut(s) 279, 764
Lsp1109I GCAGC 3 cut(s) 604, 616, 638
LweI GCATC 4 cut(s) 31, 81, 532, 727
MaeI CTAG 1 cut(s) 510
MaeII ACGT 2 cut(s) 138, 341
MaeIII GTNAC 1 cut(s) 512
MalI GATC 3 cut(s) 78, 95, 377
MbiI CCGCTC 1 cut(s) 167
MboI GATC 3 cut(s) 76, 93, 375
MboII GAAGA 4 cut(s) 21, 382, 508, 616
MflI RGATCY 1 cut(s) 93
MhlI GDGCHC 3 cut(s) 276, 341, 400
MluCI AATT 3 cut(s) 441, 541, 752
Mly113I GGCGCC 1 cut(s) 288
MlyI GAGTC 3 cut(s) 301, 419, 698
MmeI TCCRAC 1 cut(s) 429
Mph1103I ATGCAT 1 cut(s) 720
MroI TCCGGA 1 cut(s) 315
MroNI GCCGGC 1 cut(s) 200
MroXI GAANNNNTTC 1 cut(s) 576
MslI CAYNNNNRTG 1 cut(s) 137
MspA1I CMGCKG 1 cut(s) 629
MspI CCGG 3 cut(s) 6, 201, 316
MspR9I CCNGG 2 cut(s) 284, 639
MvaI CCWGG 2 cut(s) 284, 639
MvnI CGCG 6 cut(s) 90, 128, 279, 346, 359, 764
MwoI GCNNNNNNNGC 6 cut(s) 180, 285, 557, 617, 623, 626
NaeI GCCGGC 1 cut(s) 202
NarI GGCGCC 1 cut(s) 288
NdeII GATC 3 cut(s) 76, 93, 375
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 5 cut(s) 295, 584, 679, 718, 806
NlaIV GGNNCC 4 cut(s) 289, 650, 745, 760
NruI TCGCGA 1 cut(s) 359
NsiI ATGCAT 1 cut(s) 720
NspI RCATGY 2 cut(s) 679, 806
OliI CACNNNNGTG 1 cut(s) 137
PaeR7I CTCGAG 1 cut(s) 62
PciI ACATGT 1 cut(s) 802
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PdiI GCCGGC 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 576
PflMI CCANNNNNTGG 1 cut(s) 644
PkrI GCNGC 5 cut(s) 130, 166, 619, 628, 631
PleI GAGTC 3 cut(s) 301, 418, 698
PluTI GGCGCC 1 cut(s) 291
PmaCI CACGTG 1 cut(s) 139
PmlI CACGTG 1 cut(s) 139
PpsI GAGTC 3 cut(s) 301, 418, 698
Ppu21I YACGTR 1 cut(s) 139
PscI ACATGT 1 cut(s) 802
PshAI GACNNNNGTC 1 cut(s) 311
Psp124BI GAGCTC 1 cut(s) 276
Psp6I CCWGG 2 cut(s) 282, 637
PspCI CACGTG 1 cut(s) 139
PspGI CCWGG 2 cut(s) 282, 637
PspN4I GGNNCC 4 cut(s) 289, 650, 745, 760
PspPI GGNCC 3 cut(s) 67, 313, 402
PspXI VCTCGAGB 1 cut(s) 62
PstI CTGCAG 1 cut(s) 622
PstNI CAGNNNCTG 1 cut(s) 644
PsuI RGATCY 1 cut(s) 93
PvuII CAGCTG 1 cut(s) 629
RruI TCGCGA 1 cut(s) 359
RsaI GTAC 3 cut(s) 650, 745, 807
RsaNI GTAC 3 cut(s) 649, 744, 806
RseI CAYNNNNRTG 1 cut(s) 137
Rsr2I CGGWCCG 1 cut(s) 402
RsrII CGGWCCG 1 cut(s) 402
SacI GAGCTC 1 cut(s) 276
SatI GCNGC 5 cut(s) 129, 165, 618, 627, 630
Sau3AI GATC 3 cut(s) 76, 93, 375
Sau96I GGNCC 3 cut(s) 67, 313, 402
SchI GAGTC 3 cut(s) 301, 419, 698
ScrFI CCNGG 2 cut(s) 284, 639
SduI GDGCHC 3 cut(s) 276, 341, 400
SfaNI GCATC 4 cut(s) 31, 81, 532, 727
SfcI CTRYAG 1 cut(s) 618
SfoI GGCGCC 1 cut(s) 289
Sfr274I CTCGAG 1 cut(s) 62
SinI GGWCC 3 cut(s) 67, 313, 402
SlaI CTCGAG 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 4 cut(s) 62, 146, 334, 518
SmoI CTYRAG 4 cut(s) 62, 146, 334, 518
Sse9I AATT 3 cut(s) 441, 541, 752
SspDI GGCGCC 1 cut(s) 287
SspMI CTAG 1 cut(s) 510
SstI GAGCTC 1 cut(s) 276
StyD4I CCNGG 2 cut(s) 282, 637
StyI CCWWGG 1 cut(s) 379
TaiI ACGT 2 cut(s) 141, 344
TaqI TCGA 7 cut(s) 63, 75, 327, 413, 423, 564, 570
TaqII GACCGA 2 cut(s) 377, 390
TasI AATT 3 cut(s) 441, 541, 752
TatI WGTACW 1 cut(s) 805
TauI GCSGC 2 cut(s) 131, 167
TscAI CASTG 1 cut(s) 739
TseI GCWGC 3 cut(s) 617, 626, 629
TspDTI ATGAA 2 cut(s) 308, 569
TspGWI ACGGA 1 cut(s) 224
TspRI CASTG 1 cut(s) 739
Van91I CCANNNNNTGG 1 cut(s) 644
VpaK11BI GGWCC 3 cut(s) 67, 313, 402
XceI RCATGY 2 cut(s) 679, 806
XhoI CTCGAG 1 cut(s) 62
XmiI GTMKAC 1 cut(s) 243
XmnI GAANNNNTTC 1 cut(s) 576
XspI CTAG 1 cut(s) 510
Zsp2I ATGCAT 1 cut(s) 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.