Prupe.6G050600_v2.0.a1

Protein FLC EXPRESSOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000946
Physical Location & Seq
Forward (+)
3558071 .. 3560635
2565 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G050600.4

Sequence Viewer

Length: 843 bp
ATGGCGGGACGAGACCTTCGGCAGCTGCACCGGCTTCCTGTACTGACCAACACAACGGCGCTGGAGGACCGTATCGGCCTCCAGCACCGTGAGATCCAGGCCCTCCTCGTAGACAATCAACGGCTGGCAGCCACCCACGTGGCGCTGAAGCAGGACCTGGCAGCGGCTCAGCACGACCTCCGCCGCCTGTCGGTGGTCGCCGGTCAAGCCAAAGCCGAGAGGGACGCCGAGGTGCGCGAGGTCTACGAGAGGTCGTTGAAGCTGGACGCCGAGGTTCGCACCATCGACTCCATGGGCGCCGAGCTGGCTCGGACCCGCGCCGATATTCAGGAGCTCGGCTCCGTGCGCAAGGAGCTGGTAGCCGAGTTGCAATCGATTGAAGGAGAGGTTGCGAAAACTCGGTCCGAGTCGAAGCGAGTGGTAGACATTAAAGCCGATATTGAGACCTTCCAGCAGGAGATTAAGAAAGGAAGGGTTGCAATTGAGAATGAAACGAAAACCAGAGCTAAAAACCTTGAGCACCGGCGGGGAATGGAGAAAACCATGGCTGCCTTGGCTCGTGAGATTGAAAAACTTCATGGGGAGTTGGCCAATGCAGAGAAGAGAGCAAGGGCAGCAGTAGCAGCTGCTGCAGCAGCAAACCCAGGTCCTGGATATCCTGCAACCTATGGCGACCCTGAAATGGTATATGGAGGAAACGCGTACCCTGACCCCTATGGCATGCTGCATCAGGTATGTGTATGCCCTTGGCTTCTTGATGCTTTCCTCATATCACTCTCACTCTTGTGTTTGTTGCTACTCAGTAGATTAAAATCATTGGAAAAGATGTGCAAGAATTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

281

Amino Acids

31.05

Weight (kDa)

8.22

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 347
AccB1I GGYRCC 1 cut(s) 296
AccB7I CCANNNNNTGG 1 cut(s) 650
AccI GTMKAC 3 cut(s) 111, 243, 423
AccII CGCG 3 cut(s) 237, 318, 701
AciI CCGC 6 cut(s) 5, 164, 181, 184, 316, 526
AclWI GGATC 1 cut(s) 88
AcoI YGGCCR 1 cut(s) 588
AcuI CTGAAG 1 cut(s) 167
AcvI CACGTG 1 cut(s) 139
AcyI GRCGYC 3 cut(s) 225, 267, 297
AfaI GTAC 2 cut(s) 42, 704
AfiI CCNNNNNNNGG 5 cut(s) 163, 190, 193, 650, 682
AflIII ACRYGT 1 cut(s) 699
AgsI TTSAA 3 cut(s) 259, 380, 569
AjnI CCWGG 4 cut(s) 96, 156, 643, 649
AleI CACNNNNGTG 2 cut(s) 137, 784
AluBI AGCT 7 cut(s) 25, 262, 304, 334, 355, 506, 626
AluI AGCT 7 cut(s) 25, 262, 304, 334, 355, 506, 626
Alw21I GWGCWC 2 cut(s) 336, 522
Alw26I GTCTC 2 cut(s) 6, 437
AlwI GGATC 1 cut(s) 88
AlwNI CAGNNNCTG 3 cut(s) 157, 629, 650
AoxI GGCC 3 cut(s) 76, 99, 588
ArsI GACNNNNNNTTYG 3 cut(s) 32, 386, 418
Asp700I GAANNNNTTC 1 cut(s) 573
AspLEI GCGC 6 cut(s) 61, 145, 237, 299, 320, 348
AspS9I GGNCC 6 cut(s) 67, 100, 154, 312, 402, 647
AvaII GGWCC 5 cut(s) 67, 154, 312, 402, 647
BalI TGGCCA 1 cut(s) 590
BanI GGYRCC 1 cut(s) 296
BanII GRGCYC 1 cut(s) 336
BauI CACGAG 1 cut(s) 558
BbrPI CACGTG 1 cut(s) 139
Bbv12I GWGCWC 2 cut(s) 336, 522
BccI CCATC 1 cut(s) 290
BceAI ACGGC 2 cut(s) 72, 137
BciT130I CCWGG 4 cut(s) 98, 158, 645, 651
BcoDI GTCTC 2 cut(s) 6, 437
BfmI CTRYAG 1 cut(s) 630
BfoI RGCGCY 3 cut(s) 62, 146, 300
BglI GCCNNNNNGGC 1 cut(s) 305
BlpI GCTNAGC 1 cut(s) 168
Bme1390I CCNGG 4 cut(s) 98, 158, 645, 651
Bme18I GGWCC 5 cut(s) 67, 154, 312, 402, 647
BmgT120I GGNCC 6 cut(s) 67, 100, 154, 312, 402, 647
BmiI GGNNCC 3 cut(s) 298, 314, 340
BmrFI CCNGG 4 cut(s) 98, 158, 645, 651
BmsI GCATC 2 cut(s) 736, 748
BplI GAGNNNNNCTC 2 cut(s) 323, 355
BpmI CTGGAG 2 cut(s) 65, 83
Bpu1102I GCTNAGC 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 536
Bsa29I ATCGAT 1 cut(s) 374
BsaAI YACGTR 1 cut(s) 139
BsaBI GATNNNNATC 1 cut(s) 811
BsaHI GRCGYC 3 cut(s) 225, 267, 297
BsaI GGTCTC 2 cut(s) 6, 437
BsaJI CCNNGG 7 cut(s) 228, 270, 291, 543, 552, 643, 746
Bsc4I CCNNNNNNNGG 5 cut(s) 163, 190, 193, 650, 682
Bse118I RCCGGY 3 cut(s) 30, 200, 522
Bse8I GATNNNNATC 1 cut(s) 811
BseBI CCWGG 4 cut(s) 98, 158, 645, 651
BseCI ATCGAT 1 cut(s) 374
BseDI CCNNGG 7 cut(s) 228, 270, 291, 543, 552, 643, 746
BseJI GATNNNNATC 1 cut(s) 811
BseLI CCNNNNNNNGG 5 cut(s) 163, 190, 193, 650, 682
BseMII CTCAG 2 cut(s) 182, 814
BseRI GAGGAG 1 cut(s) 95
BsgI GTGCAG 1 cut(s) 11
Bsh1236I CGCG 3 cut(s) 237, 318, 701
BshFI GGCC 3 cut(s) 78, 101, 590
BshNI GGYRCC 1 cut(s) 296
BshVI ATCGAT 1 cut(s) 374
BsiHKAI GWGCWC 2 cut(s) 336, 522
BsiSI CCGG 3 cut(s) 31, 201, 523
BslFI GGGAC 2 cut(s) 21, 236
BslI CCNNNNNNNGG 5 cut(s) 163, 190, 193, 650, 682
BsmAI GTCTC 2 cut(s) 6, 437
BsmFI GGGAC 2 cut(s) 21, 236
BsnI GGCC 3 cut(s) 78, 101, 590
Bso31I GGTCTC 2 cut(s) 6, 437
Bsp1286I GDGCHC 2 cut(s) 336, 522
Bsp143I GATC 1 cut(s) 93
Bsp1720I GCTNAGC 1 cut(s) 168
Bsp19I CCATGG 2 cut(s) 291, 543
BspACI CCGC 6 cut(s) 5, 164, 181, 184, 316, 526
BspANI GGCC 3 cut(s) 78, 101, 590
BspCNI CTCAG 2 cut(s) 181, 813
BspDI ATCGAT 1 cut(s) 374
BspFNI CGCG 3 cut(s) 237, 318, 701
BspLI GGNNCC 3 cut(s) 298, 314, 340
BspMAI CTGCAG 1 cut(s) 634
BspPI GGATC 1 cut(s) 88
BspT107I GGYRCC 1 cut(s) 296
BspTNI GGTCTC 2 cut(s) 6, 437
BsrFI RCCGGY 3 cut(s) 30, 200, 522
BssAI RCCGGY 3 cut(s) 30, 200, 522
BssECI CCNNGG 7 cut(s) 228, 270, 291, 543, 552, 643, 746
BssMI GATC 1 cut(s) 93
BssNI GRCGYC 3 cut(s) 225, 267, 297
BssSI CACGAG 1 cut(s) 558
BssT1I CCWWGG 4 cut(s) 291, 543, 552, 746
Bst2BI CACGAG 1 cut(s) 558
Bst2UI CCWGG 4 cut(s) 98, 158, 645, 651
Bst4CI ACNGT 2 cut(s) 71, 89
Bst6I CTCTTC 1 cut(s) 596
BstACI GRCGYC 3 cut(s) 225, 267, 297
BstAPI GCANNNNNTGC 1 cut(s) 629
BstBAI YACGTR 1 cut(s) 139
BstC8I GCNNGC 3 cut(s) 126, 306, 722
BstDEI CTNAG 2 cut(s) 168, 800
BstDSI CCRYGG 2 cut(s) 291, 543
BstFNI CGCG 3 cut(s) 237, 318, 701
BstH2I RGCGCY 3 cut(s) 62, 146, 300
BstHHI GCGC 6 cut(s) 61, 145, 237, 299, 320, 348
BstKTI GATC 1 cut(s) 96
BstMAI GTCTC 2 cut(s) 6, 437
BstMBI GATC 1 cut(s) 93
BstNI CCWGG 4 cut(s) 98, 158, 645, 651
BstNSI RCATGY 1 cut(s) 724
BstSCI CCNGG 4 cut(s) 96, 156, 643, 649
BstSFI CTRYAG 1 cut(s) 630
BstUI CGCG 3 cut(s) 237, 318, 701
BstX2I RGATCY 1 cut(s) 93
BstXI CCANNNNNNTGG 1 cut(s) 139
BstYI RGATCY 1 cut(s) 93
Bsu15I ATCGAT 1 cut(s) 374
BsuRI GGCC 3 cut(s) 78, 101, 590
BsuTUI ATCGAT 1 cut(s) 374
BtgI CCRYGG 2 cut(s) 291, 543
Cac8I GCNNGC 3 cut(s) 126, 306, 722
CaiI CAGNNNCTG 3 cut(s) 157, 629, 650
CfoI GCGC 6 cut(s) 61, 145, 237, 299, 320, 348
Cfr10I RCCGGY 3 cut(s) 30, 200, 522
Cfr13I GGNCC 6 cut(s) 67, 100, 154, 312, 402, 647
ClaI ATCGAT 1 cut(s) 374
CpoI CGGWCCG 1 cut(s) 402
CseI GACGC 2 cut(s) 233, 275
Csp6I GTAC 2 cut(s) 41, 703
CspI CGGWCCG 1 cut(s) 402
CviAII CATG 4 cut(s) 292, 544, 578, 721
CviQI GTAC 2 cut(s) 41, 703
DdeI CTNAG 2 cut(s) 168, 800
DinI GGCGCC 1 cut(s) 298
DpnI GATC 1 cut(s) 95
DpnII GATC 1 cut(s) 93
EaeI YGGCCR 1 cut(s) 588
Eam1104I CTCTTC 1 cut(s) 596
EarI CTCTTC 1 cut(s) 596
EciI GGCGGA 1 cut(s) 170
Ecl136II GAGCTC 1 cut(s) 334
Eco130I CCWWGG 4 cut(s) 291, 543, 552, 746
Eco24I GRGCYC 1 cut(s) 336
Eco31I GGTCTC 2 cut(s) 6, 437
Eco32I GATATC 1 cut(s) 656
Eco47I GGWCC 5 cut(s) 67, 154, 312, 402, 647
Eco53kI GAGCTC 1 cut(s) 334
Eco57I CTGAAG 1 cut(s) 167
Eco72I CACGTG 1 cut(s) 139
EcoICRI GAGCTC 1 cut(s) 334
EcoO109I RGGNCCY 3 cut(s) 100, 154, 647
EcoRII CCWGG 4 cut(s) 96, 156, 643, 649
EcoRV GATATC 1 cut(s) 656
EcoT14I CCWWGG 4 cut(s) 291, 543, 552, 746
EcoT38I GRGCYC 1 cut(s) 336
EgeI GGCGCC 1 cut(s) 298
EheI GGCGCC 1 cut(s) 298
ErhI CCWWGG 4 cut(s) 291, 543, 552, 746
FaeI CATG 4 cut(s) 295, 547, 581, 724
FaqI GGGAC 2 cut(s) 21, 236
FatI CATG 4 cut(s) 291, 543, 577, 720
FauI CCCGC 2 cut(s) 323, 519
FblI GTMKAC 3 cut(s) 111, 243, 423
FriOI GRGCYC 1 cut(s) 336
FspI TGCGCA 1 cut(s) 347
GlaI GCGC 6 cut(s) 60, 144, 236, 298, 319, 347
GsuI CTGGAG 2 cut(s) 65, 83
HaeII RGCGCY 3 cut(s) 62, 146, 300
HaeIII GGCC 3 cut(s) 78, 101, 590
HapII CCGG 3 cut(s) 31, 201, 523
HgaI GACGC 2 cut(s) 233, 275
HhaI GCGC 6 cut(s) 61, 145, 237, 299, 320, 348
Hin1I GRCGYC 3 cut(s) 225, 267, 297
Hin1II CATG 4 cut(s) 295, 547, 581, 724
Hin6I GCGC 6 cut(s) 59, 143, 235, 297, 318, 346
HinP1I GCGC 6 cut(s) 59, 143, 235, 297, 318, 346
HinfI GANTC 2 cut(s) 287, 407
HpaII CCGG 3 cut(s) 31, 201, 523
Hpy166II GTNNAC 3 cut(s) 112, 244, 424
Hpy188I TCNGA 2 cut(s) 312, 406
Hpy188III TCNNGA 3 cut(s) 329, 560, 755
Hpy8I GTNNAC 3 cut(s) 112, 244, 424
HpyAV CCTTC 4 cut(s) 26, 374, 457, 465
HpyCH4III ACNGT 2 cut(s) 71, 89
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 8 cut(s) 28, 370, 479, 596, 632, 662, 727, 831
HpyF3I CTNAG 2 cut(s) 168, 800
HpySE526I ACGT 1 cut(s) 138
Hsp92I GRCGYC 3 cut(s) 225, 267, 297
Hsp92II CATG 4 cut(s) 295, 547, 581, 724
HspAI GCGC 6 cut(s) 59, 143, 235, 297, 318, 346
KasI GGCGCC 1 cut(s) 296
Kzo9I GATC 1 cut(s) 93
LmnI GCTCC 3 cut(s) 331, 344, 352
LweI GCATC 2 cut(s) 736, 748
MaeII ACGT 1 cut(s) 138
MalI GATC 1 cut(s) 95
MboI GATC 1 cut(s) 93
MboII GAAGA 1 cut(s) 613
MfeI CAATTG 1 cut(s) 480
MflI RGATCY 1 cut(s) 93
MhlI GDGCHC 2 cut(s) 336, 522
MlsI TGGCCA 1 cut(s) 590
MluCI AATT 2 cut(s) 480, 835
MluI ACGCGT 1 cut(s) 699
MluNI TGGCCA 1 cut(s) 590
Mly113I GGCGCC 1 cut(s) 297
MlyI GAGTC 2 cut(s) 281, 416
Mox20I TGGCCA 1 cut(s) 590
MroXI GAANNNNTTC 1 cut(s) 573
MscI TGGCCA 1 cut(s) 590
MseI TTAA 3 cut(s) 429, 462, 809
MslI CAYNNNNRTG 2 cut(s) 137, 784
Msp20I TGGCCA 1 cut(s) 590
MspA1I CMGCKG 3 cut(s) 25, 164, 626
MspI CCGG 3 cut(s) 31, 201, 523
MspR9I CCNGG 4 cut(s) 98, 158, 645, 651
MunI CAATTG 1 cut(s) 480
MvaI CCWGG 4 cut(s) 98, 158, 645, 651
MvnI CGCG 3 cut(s) 237, 318, 701
NarI GGCGCC 1 cut(s) 297
NcoI CCATGG 2 cut(s) 291, 543
NdeII GATC 1 cut(s) 93
NlaIII CATG 4 cut(s) 295, 547, 581, 724
NlaIV GGNNCC 3 cut(s) 298, 314, 340
NmeAIII GCCGAG 6 cut(s) 241, 253, 295, 315, 325, 388
NsbI TGCGCA 1 cut(s) 347
NspI RCATGY 1 cut(s) 724
OliI CACNNNNGTG 2 cut(s) 137, 784
PaeI GCATGC 1 cut(s) 724
PdmI GAANNNNTTC 1 cut(s) 573
PflMI CCANNNNNTGG 1 cut(s) 650
PfoI TCCNGGA 1 cut(s) 649
PleI GAGTC 2 cut(s) 281, 415
PluTI GGCGCC 1 cut(s) 300
PmaCI CACGTG 1 cut(s) 139
PmlI CACGTG 1 cut(s) 139
PpsI GAGTC 2 cut(s) 281, 415
Ppu21I YACGTR 1 cut(s) 139
PpuMI RGGWCCY 2 cut(s) 154, 647
Psp124BI GAGCTC 1 cut(s) 336
Psp5II RGGWCCY 2 cut(s) 154, 647
Psp6I CCWGG 4 cut(s) 96, 156, 643, 649
PspCI CACGTG 1 cut(s) 139
PspGI CCWGG 4 cut(s) 96, 156, 643, 649
PspN4I GGNNCC 3 cut(s) 298, 314, 340
PspPI GGNCC 6 cut(s) 67, 100, 154, 312, 402, 647
PspPPI RGGWCCY 2 cut(s) 154, 647
PstI CTGCAG 1 cut(s) 634
PstNI CAGNNNCTG 3 cut(s) 157, 629, 650
PsuI RGATCY 1 cut(s) 93
PvuII CAGCTG 2 cut(s) 25, 626
RsaI GTAC 2 cut(s) 42, 704
RsaNI GTAC 2 cut(s) 41, 703
RseI CAYNNNNRTG 2 cut(s) 137, 784
Rsr2I CGGWCCG 1 cut(s) 402
RsrII CGGWCCG 1 cut(s) 402
SacI GAGCTC 1 cut(s) 336
SaqAI TTAA 3 cut(s) 429, 462, 809
Sau3AI GATC 1 cut(s) 93
Sau96I GGNCC 6 cut(s) 67, 100, 154, 312, 402, 647
SchI GAGTC 2 cut(s) 281, 416
ScrFI CCNGG 4 cut(s) 98, 158, 645, 651
SduI GDGCHC 2 cut(s) 336, 522
SfaNI GCATC 2 cut(s) 736, 748
SfcI CTRYAG 1 cut(s) 630
SfoI GGCGCC 1 cut(s) 298
SgrAI CRCCGGYG 1 cut(s) 522
SinI GGWCC 5 cut(s) 67, 154, 312, 402, 647
SmiMI CAYNNNNRTG 2 cut(s) 137, 784
SmlI CTYRAG 1 cut(s) 515
SmoI CTYRAG 1 cut(s) 515
SphI GCATGC 1 cut(s) 724
Sse9I AATT 2 cut(s) 480, 835
SsiI CCGC 6 cut(s) 5, 164, 181, 184, 316, 526
SspDI GGCGCC 1 cut(s) 296
SstI GAGCTC 1 cut(s) 336
StyD4I CCNGG 4 cut(s) 96, 156, 643, 649
StyI CCWWGG 4 cut(s) 291, 543, 552, 746
TaaI ACNGT 2 cut(s) 71, 89
TaiI ACGT 1 cut(s) 141
TaqI TCGA 3 cut(s) 285, 374, 410
TaqII GACCGA 1 cut(s) 390
TasI AATT 2 cut(s) 480, 835
TatI WGTACW 1 cut(s) 40
TauI GCSGC 2 cut(s) 167, 186
Tru1I TTAA 3 cut(s) 429, 462, 809
Tru9I TTAA 3 cut(s) 429, 462, 809
TspDTI ATGAA 2 cut(s) 504, 566
TspGWI ACGGA 1 cut(s) 331
Van91I CCANNNNNTGG 1 cut(s) 650
VpaK11BI GGWCC 5 cut(s) 67, 154, 312, 402, 647
XceI RCATGY 1 cut(s) 724
XcmI CCANNNNNNNNNTGG 2 cut(s) 289, 550
XmiI GTMKAC 3 cut(s) 111, 243, 423
XmnI GAANNNNTTC 1 cut(s) 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.