Rroxscaffold_3G00252320

Protein FLC EXPRESSOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000946
Physical Location & Seq
Forward (+)
46341424 .. 46342159
736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00252320.1

Sequence Viewer

Length: 558 bp
ATGAGATCAGTTATGGTCGGAAGAAAGCCTCAACATCTCCACCACCTTCCCCAAATCGTCAACAACACCGCATCGATCTCTAGCAACGCGAGATCCGGTCCCTCCCGGGGACAATCAACTCCTCGCGGCCACCCACGTGGCGCTCAAGCAGACCTAACCACCGCTCGCAGTGACCTCCGCAACCCGAAGGTCGTCGCCGCCAAATCAAATCCGAGAGAAACGACGAAGTCCATGAGTCGCGGGATGGAGCTCGTGACCAAGTTGAAGACTATCCAAGGAGAGAATGAGAGGGCTCGGTCCGACGAGTCGAAGAAAATCGAAGCCATTAGAGACGAAATTGAGACTTTGAAGCCGGAGATTGAGAAAGGAAGAGCTGGAGACGAAATTGAGACGAAATTGAGAGAGAATTTTGAAATTGTCGAGATCGACGATCTTTGTAGATGGTTCGGTGCGGACCTCGTCAATGATGGTGGGCTCGAGGTCTATGAAGATGGGGCGTGGGACGTGCTTGCCGCACCGGCTCAGGAGAAGAAGGTGTTGAAGGCACCGAAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

20.5

Weight (kDa)

8.67

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 544
AccBSI CCGCTC 1 cut(s) 164
AccII CGCG 3 cut(s) 89, 126, 240
AciI CCGC 8 cut(s) 69, 126, 162, 178, 198, 240, 452, 513
AclWI GGATC 1 cut(s) 87
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 1 cut(s) 406
AcvI CACGTG 1 cut(s) 137
AfiI CCNNNNNNNGG 1 cut(s) 107
AgsI TTSAA 4 cut(s) 265, 349, 413, 541
AjiI CACGTC 1 cut(s) 505
AleI CACNNNNGTG 1 cut(s) 135
AluBI AGCT 2 cut(s) 250, 374
AluI AGCT 2 cut(s) 250, 374
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 4 cut(s) 324, 335, 372, 383
AlwI GGATC 1 cut(s) 87
Ama87I CYCGRG 2 cut(s) 105, 476
AoxI GGCC 1 cut(s) 127
ApoI RAATTY 1 cut(s) 406
AspLEI GCGC 1 cut(s) 143
AspS9I GGNCC 3 cut(s) 98, 297, 454
AsuC2I CCSGG 2 cut(s) 106, 107
AvaI CYCGRG 2 cut(s) 105, 476
AvaII GGWCC 3 cut(s) 98, 297, 454
BanI GGYRCC 1 cut(s) 544
BanII GRGCYC 3 cut(s) 252, 295, 477
BauI CACGAG 1 cut(s) 251
BbrPI CACGTG 1 cut(s) 137
BbsI GAAGAC 1 cut(s) 272
Bbv12I GWGCWC 1 cut(s) 252
BccI CCATC 4 cut(s) 238, 435, 461, 485
BcnI CCSGG 2 cut(s) 106, 107
BcoDI GTCTC 4 cut(s) 324, 335, 372, 383
BfaI CTAG 1 cut(s) 81
BfoI RGCGCY 1 cut(s) 144
BglI GCCNNNNNGGC 1 cut(s) 518
BisI GCNGC 3 cut(s) 127, 198, 513
BlsI GCNGC 3 cut(s) 128, 199, 514
Bme1390I CCNGG 2 cut(s) 106, 107
Bme18I GGWCC 3 cut(s) 98, 297, 454
BmeT110I CYCGRG 2 cut(s) 105, 476
BmgBI CACGTC 1 cut(s) 505
BmgT120I GGNCC 3 cut(s) 98, 297, 454
BmiI GGNNCC 2 cut(s) 100, 546
BmrFI CCNGG 2 cut(s) 106, 107
BmsI GCATC 1 cut(s) 80
BpiI GAAGAC 1 cut(s) 272
BpmI CTGGAG 1 cut(s) 396
Bpu10I CCTNAGC 1 cut(s) 522
BpuEI CTTGAG 1 cut(s) 129
BpuMI CCSGG 2 cut(s) 106, 107
Bsa29I ATCGAT 1 cut(s) 74
BsaAI YACGTR 1 cut(s) 137
BsaJI CCNNGG 3 cut(s) 105, 106, 274
BsaWI WCCGGW 1 cut(s) 95
Bsc4I CCNNNNNNNGG 1 cut(s) 107
Bse118I RCCGGY 1 cut(s) 517
BseCI ATCGAT 1 cut(s) 74
BseDI CCNNGG 3 cut(s) 105, 106, 274
BseGI GGATG 1 cut(s) 249
BseLI CCNNNNNNNGG 1 cut(s) 107
BseMII CTCAG 1 cut(s) 536
BseRI GAGGAG 1 cut(s) 111
Bsh1236I CGCG 3 cut(s) 89, 126, 240
BshFI GGCC 1 cut(s) 129
BshNI GGYRCC 1 cut(s) 544
BshVI ATCGAT 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 252
BsiHKCI CYCGRG 2 cut(s) 105, 476
BsiSI CCGG 4 cut(s) 96, 106, 353, 518
BslFI GGGAC 3 cut(s) 84, 123, 515
BslI CCNNNNNNNGG 1 cut(s) 107
BsmAI GTCTC 4 cut(s) 324, 335, 372, 383
BsmBI CGTCTC 3 cut(s) 324, 372, 383
BsmFI GGGAC 3 cut(s) 84, 123, 515
BsnI GGCC 1 cut(s) 129
BsoBI CYCGRG 2 cut(s) 105, 476
Bsp1286I GDGCHC 3 cut(s) 252, 295, 477
Bsp143I GATC 5 cut(s) 5, 75, 92, 423, 430
BspACI CCGC 8 cut(s) 69, 126, 162, 178, 198, 240, 452, 513
BspANI GGCC 1 cut(s) 129
BspCNI CTCAG 1 cut(s) 535
BspDI ATCGAT 1 cut(s) 74
BspFNI CGCG 3 cut(s) 89, 126, 240
BspLI GGNNCC 2 cut(s) 100, 546
BspPI GGATC 1 cut(s) 87
BspQI GCTCTTC 1 cut(s) 364
BspT107I GGYRCC 1 cut(s) 544
BsrBI CCGCTC 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 517
BssAI RCCGGY 1 cut(s) 517
BssECI CCNNGG 3 cut(s) 105, 106, 274
BssMI GATC 5 cut(s) 5, 75, 92, 423, 430
BssSI CACGAG 1 cut(s) 251
BssT1I CCWWGG 1 cut(s) 274
Bst2BI CACGAG 1 cut(s) 251
Bst6I CTCTTC 1 cut(s) 364
BstBAI YACGTR 1 cut(s) 137
BstC8I GCNNGC 2 cut(s) 166, 510
BstDEI CTNAG 1 cut(s) 522
BstF5I GGATG 1 cut(s) 249
BstFNI CGCG 3 cut(s) 89, 126, 240
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 5 cut(s) 8, 78, 95, 426, 433
BstMAI GTCTC 4 cut(s) 324, 335, 372, 383
BstMBI GATC 5 cut(s) 5, 75, 92, 423, 430
BstMWI GCNNNNNNNGC 1 cut(s) 518
BstSCI CCNGG 2 cut(s) 104, 105
BstUI CGCG 3 cut(s) 89, 126, 240
BstV2I GAAGAC 1 cut(s) 272
BstX2I RGATCY 1 cut(s) 92
BstXI CCANNNNNNTGG 1 cut(s) 137
BstYI RGATCY 1 cut(s) 92
Bsu15I ATCGAT 1 cut(s) 74
BsuRI GGCC 1 cut(s) 129
BsuTUI ATCGAT 1 cut(s) 74
BtrI CACGTC 1 cut(s) 505
BtsCI GGATG 1 cut(s) 249
BtsI GCAGTG 1 cut(s) 175
BtsIMutI CAGTG 1 cut(s) 175
Cac8I GCNNGC 2 cut(s) 166, 510
CfoI GCGC 1 cut(s) 143
Cfr10I RCCGGY 1 cut(s) 517
Cfr13I GGNCC 3 cut(s) 98, 297, 454
Cfr9I CCCGGG 1 cut(s) 105
ClaI ATCGAT 1 cut(s) 74
CpoI CGGWCCG 1 cut(s) 297
CspCI CAANNNNNGTGG 2 cut(s) 451, 486
CspI CGGWCCG 1 cut(s) 297
CviAII CATG 1 cut(s) 232
CviJI RGCY 9 cut(s) 28, 129, 250, 293, 323, 352, 374, 475, 521
CviKI_1 RGCY 9 cut(s) 28, 129, 250, 293, 323, 352, 374, 475, 521
DdeI CTNAG 1 cut(s) 522
DpnI GATC 5 cut(s) 7, 77, 94, 425, 432
DpnII GATC 5 cut(s) 5, 75, 92, 423, 430
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 1 cut(s) 364
EarI CTCTTC 1 cut(s) 364
Ecl136II GAGCTC 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 274
Eco24I GRGCYC 3 cut(s) 252, 295, 477
Eco47I GGWCC 3 cut(s) 98, 297, 454
Eco53kI GAGCTC 1 cut(s) 250
Eco72I CACGTG 1 cut(s) 137
Eco88I CYCGRG 2 cut(s) 105, 476
EcoICRI GAGCTC 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 274
EcoT38I GRGCYC 3 cut(s) 252, 295, 477
ErhI CCWWGG 1 cut(s) 274
Esp3I CGTCTC 3 cut(s) 324, 372, 383
FaeI CATG 1 cut(s) 235
FaiI YATR 3 cut(s) 14, 233, 486
FaqI GGGAC 3 cut(s) 84, 123, 515
FatI CATG 1 cut(s) 231
FauI CCCGC 1 cut(s) 233
Fnu4HI GCNGC 3 cut(s) 127, 198, 513
FokI GGATG 1 cut(s) 256
FriOI GRGCYC 3 cut(s) 252, 295, 477
Fsp4HI GCNGC 3 cut(s) 127, 198, 513
FspBI CTAG 1 cut(s) 81
GlaI GCGC 1 cut(s) 142
GluI GCNGC 3 cut(s) 127, 198, 513
GsuI CTGGAG 1 cut(s) 396
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 1 cut(s) 129
HapII CCGG 4 cut(s) 96, 106, 353, 518
HhaI GCGC 1 cut(s) 143
Hin1II CATG 1 cut(s) 235
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HinfI GANTC 2 cut(s) 235, 305
HpaII CCGG 4 cut(s) 96, 106, 353, 518
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 3 cut(s) 20, 213, 301
Hpy188III TCNNGA 3 cut(s) 253, 421, 524
Hpy8I GTNNAC 1 cut(s) 61
Hpy99I CGWCG 4 cut(s) 197, 226, 305, 431
HpyAV CCTTC 4 cut(s) 56, 181, 526, 535
HpyCH4IV ACGT 2 cut(s) 136, 504
HpyF10VI GCNNNNNNNGC 1 cut(s) 518
HpyF3I CTNAG 1 cut(s) 522
HpySE526I ACGT 2 cut(s) 136, 504
Hsp92II CATG 1 cut(s) 235
HspAI GCGC 1 cut(s) 141
Kzo9I GATC 5 cut(s) 5, 75, 92, 423, 430
LguI GCTCTTC 1 cut(s) 364
LmnI GCTCC 1 cut(s) 247
LpnPI CCDG 6 cut(s) 109, 119, 360, 366, 509, 531
LweI GCATC 1 cut(s) 80
MaeI CTAG 1 cut(s) 81
MaeII ACGT 2 cut(s) 136, 504
MaeIII GTNAC 2 cut(s) 170, 253
MalI GATC 5 cut(s) 7, 77, 94, 425, 432
MbiI CCGCTC 1 cut(s) 164
MboI GATC 5 cut(s) 5, 75, 92, 423, 430
MboII GAAGA 6 cut(s) 33, 277, 322, 381, 500, 541
MflI RGATCY 1 cut(s) 92
MhlI GDGCHC 3 cut(s) 252, 295, 477
MluCI AATT 5 cut(s) 336, 384, 395, 406, 414
MlyI GAGTC 2 cut(s) 244, 314
MmeI TCCRAC 1 cut(s) 324
MnlI CCTC 7 cut(s) 39, 112, 132, 185, 282, 467, 472
MslI CAYNNNNRTG 1 cut(s) 135
MspI CCGG 4 cut(s) 96, 106, 353, 518
MspR9I CCNGG 2 cut(s) 106, 107
MvnI CGCG 3 cut(s) 89, 126, 240
MwoI GCNNNNNNNGC 1 cut(s) 518
NciI CCSGG 2 cut(s) 106, 107
NdeII GATC 5 cut(s) 5, 75, 92, 423, 430
NlaIII CATG 1 cut(s) 235
NlaIV GGNNCC 2 cut(s) 100, 546
NmuCI GTSAC 2 cut(s) 170, 253
OliI CACNNNNGTG 1 cut(s) 135
PaeR7I CTCGAG 1 cut(s) 476
PciSI GCTCTTC 1 cut(s) 364
PcsI WCGNNNNNNNCGW 1 cut(s) 426
PflFI GACNNNGTC 2 cut(s) 226, 458
PkrI GCNGC 3 cut(s) 128, 199, 514
PleI GAGTC 2 cut(s) 243, 313
PmaCI CACGTG 1 cut(s) 137
PmlI CACGTG 1 cut(s) 137
PpsI GAGTC 2 cut(s) 243, 313
Ppu21I YACGTR 1 cut(s) 137
Psp124BI GAGCTC 1 cut(s) 252
PspCI CACGTG 1 cut(s) 137
PspN4I GGNNCC 2 cut(s) 100, 546
PspPI GGNCC 3 cut(s) 98, 297, 454
PspXI VCTCGAGB 1 cut(s) 476
PsuI RGATCY 1 cut(s) 92
PsyI GACNNNGTC 2 cut(s) 226, 458
RseI CAYNNNNRTG 1 cut(s) 135
Rsr2I CGGWCCG 1 cut(s) 297
RsrII CGGWCCG 1 cut(s) 297
SacI GAGCTC 1 cut(s) 252
SapI GCTCTTC 1 cut(s) 364
SatI GCNGC 3 cut(s) 127, 198, 513
Sau3AI GATC 5 cut(s) 5, 75, 92, 423, 430
Sau96I GGNCC 3 cut(s) 98, 297, 454
SchI GAGTC 2 cut(s) 244, 314
ScrFI CCNGG 2 cut(s) 106, 107
SduI GDGCHC 3 cut(s) 252, 295, 477
SfaNI GCATC 1 cut(s) 80
Sfr274I CTCGAG 1 cut(s) 476
SinI GGWCC 3 cut(s) 98, 297, 454
SlaI CTCGAG 1 cut(s) 476
SmaI CCCGGG 1 cut(s) 107
SmiMI CAYNNNNRTG 1 cut(s) 135
SmlI CTYRAG 2 cut(s) 144, 476
SmoI CTYRAG 2 cut(s) 144, 476
Sse9I AATT 5 cut(s) 336, 384, 395, 406, 414
SsiI CCGC 8 cut(s) 69, 126, 162, 178, 198, 240, 452, 513
SspMI CTAG 1 cut(s) 81
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 104, 105
StyI CCWWGG 1 cut(s) 274
TaiI ACGT 2 cut(s) 139, 507
TaqI TCGA 6 cut(s) 74, 308, 318, 420, 426, 477
TaqII GACCGA 1 cut(s) 285
TasI AATT 5 cut(s) 336, 384, 395, 406, 414
TauI GCSGC 3 cut(s) 129, 200, 515
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 2 cut(s) 170, 253
Tsp45I GTSAC 2 cut(s) 170, 253
TspDTI ATGAA 1 cut(s) 501
TspMI CCCGGG 1 cut(s) 105
TspRI CASTG 1 cut(s) 175
Tth111I GACNNNGTC 2 cut(s) 226, 458
VpaK11BI GGWCC 3 cut(s) 98, 297, 454
XapI RAATTY 1 cut(s) 406
XhoI CTCGAG 1 cut(s) 476
XmaI CCCGGG 1 cut(s) 105
XspI CTAG 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.