RLG00000009353

Protein FLC EXPRESSOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000946
Physical Location & Seq
Reverse (-)
51424212 .. 51425516
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009353

Sequence Viewer

Length: 627 bp
ATGTCTAAGCAATCCGCCGCCGGCCGCGACATCTCCGTCATCGCCAAGTCCGCCATCGAAGAGATCGTCGCCTCCGCCGTCTGCAAGAAGCTCGACTTCGACCTCATCCGCTCCACGCGCCTCACCGCCGACCTCTGGGACACTGTCTGCATCGGCGTCCTCACCGATCTCGACTTCCCCGACCTTGACGTCAGCGCAGCTGTCGTCTCGATCCTCACCGCCATCCCCTCCTACCGCCTCCCCAAGCTCATCTTCGACACCAAGAAACCCCTAAAACTCTCCTCCCCTCCCCCCCCCCCCCCAAAAAATAAAAATCCGAGCGACCTCCGTAACCTGAAGGCCGTCGCCGGCCAAATCAAATCCGAGAGGAATACCGACGTGCGAGAGGTCTACGACAGGTCGTTGAAGCTGGACGACGAGCTCCGCCACCTGGACGCCATGAACGCCGAGTTGACTCTGGTCCGGAACGAAATCGAGGACTTGAGCACGTCGTGGATAGAGCTCGCGATCGAGTTGAAGACGATCCAAGGAGAGATTGAGAGGGCTCGCTCCGACGAGTCGAAGCAAATTGAAGTCATTAGAGACGAAATTGAGACTTTGAAGCTGGAGATTGAGAAAGGAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

209

Amino Acids

23.28

Weight (kDa)

5.07

Isoelectric Point (pI)

58.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 35, 188
AatII GACGTC 1 cut(s) 192
AccBSI CCGCTC 1 cut(s) 111
AccI GTMKAC 1 cut(s) 390
AccII CGCG 3 cut(s) 27, 118, 506
AccIII TCCGGA 1 cut(s) 462
AclWI GGATC 2 cut(s) 205, 517
AcoI YGGCCR 2 cut(s) 22, 349
AcuI CTGAAG 1 cut(s) 356
AcyI GRCGYC 3 cut(s) 156, 189, 435
AdeI CACNNNGTG 1 cut(s) 492
AfiI CCNNNNNNNGG 2 cut(s) 135, 430
AgsI TTSAA 4 cut(s) 406, 517, 572, 601
AjiI CACGTC 2 cut(s) 379, 489
AjnI CCWGG 1 cut(s) 429
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 7 cut(s) 91, 200, 247, 409, 421, 502, 604
AluI AGCT 7 cut(s) 91, 200, 247, 409, 421, 502, 604
Alw21I GWGCWC 3 cut(s) 423, 488, 504
Alw26I GTCTC 3 cut(s) 211, 576, 587
AlwI GGATC 2 cut(s) 205, 517
Aor13HI TCCGGA 1 cut(s) 462
AoxI GGCC 3 cut(s) 22, 339, 349
ApeKI GCWGC 1 cut(s) 197
ArsI GACNNNNNNTTYG 2 cut(s) 51, 83
AspLEI GCGC 2 cut(s) 120, 197
AspS9I GGNCC 1 cut(s) 460
AsuHPI GGTGA 3 cut(s) 115, 154, 208
AvaII GGWCC 1 cut(s) 460
BanII GRGCYC 3 cut(s) 423, 504, 547
BbsI GAAGAC 1 cut(s) 524
Bbv12I GWGCWC 3 cut(s) 423, 488, 504
BbvI GCAGC 1 cut(s) 209
BccI CCATC 2 cut(s) 62, 230
BceAI ACGGC 2 cut(s) 62, 326
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BciT130I CCWGG 1 cut(s) 431
BcoDI GTCTC 3 cut(s) 211, 576, 587
BisI GCNGC 3 cut(s) 18, 25, 198
BlsI GCNGC 3 cut(s) 19, 26, 199
Bme1390I CCNGG 1 cut(s) 431
Bme18I GGWCC 1 cut(s) 460
BmgBI CACGTC 2 cut(s) 379, 489
BmgT120I GGNCC 1 cut(s) 460
BmrFI CCNGG 1 cut(s) 431
BmsI GCATC 1 cut(s) 159
BoxI GACNNNNGTC 1 cut(s) 458
BpiI GAAGAC 1 cut(s) 524
BpmI CTGGAG 1 cut(s) 626
BpuEI CTTGAG 1 cut(s) 502
BsaHI GRCGYC 3 cut(s) 156, 189, 435
BsaJI CCNNGG 1 cut(s) 526
BsaWI WCCGGW 1 cut(s) 462
Bsc4I CCNNNNNNNGG 2 cut(s) 135, 430
Bse118I RCCGGY 2 cut(s) 20, 347
BseAI TCCGGA 1 cut(s) 462
BseBI CCWGG 1 cut(s) 431
BseDI CCNNGG 1 cut(s) 526
BseGI GGATG 2 cut(s) 105, 222
BseLI CCNNNNNNNGG 2 cut(s) 135, 430
BseRI GAGGAG 1 cut(s) 271
BseX3I CGGCCG 1 cut(s) 22
BseXI GCAGC 1 cut(s) 209
Bsh1236I CGCG 3 cut(s) 27, 118, 506
Bsh1285I CGRYCG 2 cut(s) 25, 510
BshFI GGCC 3 cut(s) 24, 341, 351
BsiEI CGRYCG 2 cut(s) 25, 510
BsiHKAI GWGCWC 3 cut(s) 423, 488, 504
BsiSI CCGG 3 cut(s) 21, 348, 463
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 2 cut(s) 135, 430
BsmAI GTCTC 3 cut(s) 211, 576, 587
BsmBI CGTCTC 2 cut(s) 211, 576
BsmFI GGGAC 1 cut(s) 152
BsnI GGCC 3 cut(s) 24, 341, 351
Bsp1286I GDGCHC 4 cut(s) 423, 488, 504, 547
Bsp13I TCCGGA 1 cut(s) 462
Bsp143I GATC 5 cut(s) 63, 166, 210, 507, 522
Bsp68I TCGCGA 1 cut(s) 506
BspANI GGCC 3 cut(s) 24, 341, 351
BspEI TCCGGA 1 cut(s) 462
BspFNI CGCG 3 cut(s) 27, 118, 506
BspPI GGATC 2 cut(s) 205, 517
BsrBI CCGCTC 1 cut(s) 111
BsrFI RCCGGY 2 cut(s) 20, 347
BssAI RCCGGY 2 cut(s) 20, 347
BssECI CCNNGG 1 cut(s) 526
BssMI GATC 5 cut(s) 63, 166, 210, 507, 522
BssNI GRCGYC 3 cut(s) 156, 189, 435
BssT1I CCWWGG 1 cut(s) 526
Bst2UI CCWGG 1 cut(s) 431
Bst4CI ACNGT 1 cut(s) 145
Bst6I CTCTTC 1 cut(s) 54
BstACI GRCGYC 3 cut(s) 156, 189, 435
BstC8I GCNNGC 4 cut(s) 22, 349, 504, 547
BstDEI CTNAG 1 cut(s) 6
BstF5I GGATG 2 cut(s) 105, 222
BstFNI CGCG 3 cut(s) 27, 118, 506
BstHHI GCGC 2 cut(s) 120, 197
BstKTI GATC 5 cut(s) 66, 169, 213, 510, 525
BstMAI GTCTC 3 cut(s) 211, 576, 587
BstMBI GATC 5 cut(s) 63, 166, 210, 507, 522
BstMCI CGRYCG 2 cut(s) 25, 510
BstMWI GCNNNNNNNGC 3 cut(s) 50, 117, 443
BstNI CCWGG 1 cut(s) 431
BstPAI GACNNNNGTC 1 cut(s) 458
BstSCI CCNGG 1 cut(s) 429
BstUI CGCG 3 cut(s) 27, 118, 506
BstV1I GCAGC 1 cut(s) 209
BstV2I GAAGAC 1 cut(s) 524
BstZI CGGCCG 1 cut(s) 22
BsuRI GGCC 3 cut(s) 24, 341, 351
BtgZI GCGATG 1 cut(s) 25
BtrI CACGTC 2 cut(s) 379, 489
BtsCI GGATG 2 cut(s) 105, 222
BtsIMutI CAGTG 1 cut(s) 141
BtuMI TCGCGA 1 cut(s) 506
Cac8I GCNNGC 4 cut(s) 22, 349, 504, 547
CfoI GCGC 2 cut(s) 120, 197
Cfr10I RCCGGY 2 cut(s) 20, 347
Cfr13I GGNCC 1 cut(s) 460
CseI GACGC 2 cut(s) 145, 443
CviAII CATG 1 cut(s) 439
DdeI CTNAG 1 cut(s) 6
DpnI GATC 5 cut(s) 65, 168, 212, 509, 524
DpnII GATC 5 cut(s) 63, 166, 210, 507, 522
DraIII CACNNNGTG 1 cut(s) 492
DrdI GACNNNNNNGTC 2 cut(s) 35, 188
DseDI GACNNNNNNGTC 2 cut(s) 35, 188
EaeI YGGCCR 2 cut(s) 22, 349
EagI CGGCCG 1 cut(s) 22
Eam1104I CTCTTC 1 cut(s) 54
EarI CTCTTC 1 cut(s) 54
EciI GGCGGA 4 cut(s) 4, 40, 64, 413
Ecl136II GAGCTC 2 cut(s) 421, 502
EclXI CGGCCG 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 526
Eco24I GRGCYC 3 cut(s) 423, 504, 547
Eco47I GGWCC 1 cut(s) 460
Eco52I CGGCCG 1 cut(s) 22
Eco53kI GAGCTC 2 cut(s) 421, 502
Eco57I CTGAAG 1 cut(s) 356
EcoICRI GAGCTC 2 cut(s) 421, 502
EcoRII CCWGG 1 cut(s) 429
EcoT14I CCWWGG 1 cut(s) 526
EcoT38I GRGCYC 3 cut(s) 423, 504, 547
ErhI CCWWGG 1 cut(s) 526
Esp3I CGTCTC 2 cut(s) 211, 576
FaeI CATG 1 cut(s) 442
FaiI YATR 1 cut(s) 440
FalI AAGNNNNNCTT 4 cut(s) 80, 112, 236, 268
FaqI GGGAC 1 cut(s) 152
FatI CATG 1 cut(s) 438
FblI GTMKAC 1 cut(s) 390
Fnu4HI GCNGC 3 cut(s) 18, 25, 198
FokI GGATG 2 cut(s) 92, 209
FriOI GRGCYC 3 cut(s) 423, 504, 547
Fsp4HI GCNGC 3 cut(s) 18, 25, 198
GlaI GCGC 2 cut(s) 119, 196
GluI GCNGC 3 cut(s) 18, 25, 198
GsuI CTGGAG 1 cut(s) 626
HaeIII GGCC 3 cut(s) 24, 341, 351
HapII CCGG 3 cut(s) 21, 348, 463
HgaI GACGC 2 cut(s) 145, 443
HhaI GCGC 2 cut(s) 120, 197
Hin1I GRCGYC 3 cut(s) 156, 189, 435
Hin1II CATG 1 cut(s) 442
Hin6I GCGC 2 cut(s) 118, 195
HinP1I GCGC 2 cut(s) 118, 195
HincII GTYRAC 1 cut(s) 453
HindII GTYRAC 1 cut(s) 453
HinfI GANTC 2 cut(s) 454, 557
HpaII CCGG 3 cut(s) 21, 348, 463
HphI GGTGA 3 cut(s) 115, 154, 208
Hpy166II GTNNAC 2 cut(s) 391, 453
Hpy188I TCNGA 3 cut(s) 318, 364, 553
Hpy188III TCNNGA 4 cut(s) 170, 208, 463, 505
Hpy8I GTNNAC 2 cut(s) 391, 453
Hpy99I CGWCG 6 cut(s) 71, 347, 380, 419, 493, 557
HpyAV CCTTC 2 cut(s) 331, 615
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 3 cut(s) 189, 378, 488
HpyCH4V TGCA 2 cut(s) 84, 150
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 117, 443
HpyF3I CTNAG 1 cut(s) 6
HpySE526I ACGT 3 cut(s) 189, 378, 488
Hsp92I GRCGYC 3 cut(s) 156, 189, 435
Hsp92II CATG 1 cut(s) 442
HspAI GCGC 2 cut(s) 118, 195
Kpn2I TCCGGA 1 cut(s) 462
KroI GCCGGC 2 cut(s) 20, 347
KroNI GCCGGC 2 cut(s) 22, 349
Kzo9I GATC 5 cut(s) 63, 166, 210, 507, 522
LmnI GCTCC 3 cut(s) 116, 426, 554
Lsp1109I GCAGC 1 cut(s) 209
LweI GCATC 1 cut(s) 159
MaeII ACGT 3 cut(s) 189, 378, 488
MaeIII GTNAC 1 cut(s) 329
MalI GATC 5 cut(s) 65, 168, 212, 509, 524
MbiI CCGCTC 1 cut(s) 111
MboI GATC 5 cut(s) 63, 166, 210, 507, 522
MboII GAAGA 3 cut(s) 71, 244, 529
MhlI GDGCHC 4 cut(s) 423, 488, 504, 547
MluCI AATT 2 cut(s) 567, 588
MlyI GAGTC 2 cut(s) 448, 566
MmeI TCCRAC 1 cut(s) 576
MroI TCCGGA 1 cut(s) 462
MroNI GCCGGC 2 cut(s) 20, 347
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 3 cut(s) 21, 348, 463
MspR9I CCNGG 1 cut(s) 431
MvaI CCWGG 1 cut(s) 431
MvnI CGCG 3 cut(s) 27, 118, 506
MwoI GCNNNNNNNGC 3 cut(s) 50, 117, 443
NaeI GCCGGC 2 cut(s) 22, 349
NdeII GATC 5 cut(s) 63, 166, 210, 507, 522
NgoMIV GCCGGC 2 cut(s) 20, 347
NlaIII CATG 1 cut(s) 442
NmeAIII GCCGAG 1 cut(s) 472
NruI TCGCGA 1 cut(s) 506
PcsI WCGNNNNNNNCGW 3 cut(s) 63, 75, 177
PdiI GCCGGC 2 cut(s) 22, 349
PflFI GACNNNGTC 1 cut(s) 143
PkrI GCNGC 3 cut(s) 19, 26, 199
Ple19I CGATCG 1 cut(s) 510
PleI GAGTC 2 cut(s) 448, 565
PpsI GAGTC 2 cut(s) 448, 565
PshAI GACNNNNGTC 1 cut(s) 458
Psp124BI GAGCTC 2 cut(s) 423, 504
Psp6I CCWGG 1 cut(s) 429
PspGI CCWGG 1 cut(s) 429
PspPI GGNCC 1 cut(s) 460
PsyI GACNNNGTC 1 cut(s) 143
PvuI CGATCG 1 cut(s) 510
PvuII CAGCTG 1 cut(s) 200
RruI TCGCGA 1 cut(s) 506
SacI GAGCTC 2 cut(s) 423, 504
SatI GCNGC 3 cut(s) 18, 25, 198
Sau3AI GATC 5 cut(s) 63, 166, 210, 507, 522
Sau96I GGNCC 1 cut(s) 460
SchI GAGTC 2 cut(s) 448, 566
ScrFI CCNGG 1 cut(s) 431
SduI GDGCHC 4 cut(s) 423, 488, 504, 547
SfaNI GCATC 1 cut(s) 159
SinI GGWCC 1 cut(s) 460
SmlI CTYRAG 1 cut(s) 481
SmoI CTYRAG 1 cut(s) 481
Sse9I AATT 2 cut(s) 567, 588
SstI GAGCTC 2 cut(s) 423, 504
StyD4I CCNGG 1 cut(s) 429
StyI CCWWGG 1 cut(s) 526
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 3 cut(s) 192, 381, 491
TaqI TCGA 9 cut(s) 57, 93, 99, 171, 209, 255, 474, 510, 560
TasI AATT 2 cut(s) 567, 588
TauI GCSGC 2 cut(s) 20, 27
TscAI CASTG 1 cut(s) 148
TseI GCWGC 1 cut(s) 197
TspDTI ATGAA 1 cut(s) 455
TspGWI ACGGA 2 cut(s) 25, 317
TspRI CASTG 1 cut(s) 148
Tth111I GACNNNGTC 1 cut(s) 143
VpaK11BI GGWCC 1 cut(s) 460
XmiI GTMKAC 1 cut(s) 390
ZraI GACGTC 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.