FvH4_5g24500

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000996
Physical Location & Seq
Reverse (-)
15838420 .. 15843071
4652 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g24500.t8

Sequence Viewer

Length: 1185 bp
ATGGCCTCTGCCAGAATAGGCCAGTGGTTAAGTCCTCTGGTATGTTTACTCATATTGCTAAAAGCTGAAAGCCATCCTGTGGAACTCACTTTGGTCCAGAGTGCAGTTGCCAAAGGAGCTGTTTGTTTAGATGGGAGTCCACCGGCATACCACTTTGACAAGGGATTTGGTGCAGGGATTAACAATTGGTTGGTTCATATTGAGGGAGGAGGATGGTGCAAGGATGTTGAGAGTTGCGTTGAGCGGACAAAAAACTTCAGAGGTTCATCCAAACATATGGAGAAGACAATATCTTTTTCTGGGGTTCTTGGTAAAAAGGAAACTTTGAATCCAGACTTCTACAACTGGAACAGAATCAAGGTTAGGTACTGTGATGGCTCATCATTCACCGGTGATGTGGAAGCAGTAAACCCAGCTACAAATCTTCACTTCAGAGGAGGAAGGGTTTTTCTTGCTGTCATTGATGATCTCTTAGCAAAAGGAATGATGAATGCCAAAAATGCACTACTCTCTGGTTGTTCAGCTGGAGGATTGGCTTCTATTCTTCAATGTGATAACTTCCGTTCTCTCTTGCCCGCTGGAACTAATGTGAAATGCCTTGCGGATGCTGGTTACTTCATCAATGCGAAGAGTATTTCTGGATCACAACAAATTGAAGCTTTTTACAGTCAAGTGGTTGCACTACATGGTTCAGCAAAGCATTTACCTGCATCATGCACTTCAAGATTGAATCCAGGATTGTGCTTCTTCCCACAAAATGTGGTACCGACACTCCGGACCCCTATTTTTTTGGTAAATGCTGCTTATGATTCGTGGCAGATAAAGAATATTTTGGCACCAAGCGCTGCTGATCCTAAAGGCTCATGGAAAAGCTGCAAGATGGGTATAAAGAACTGCTCACCTGCACAAATTCAAACCATGCAAGATTATAGGACAACTTTCTTAAATGTACTCAATGGTGGTCTCACCTCTCCATCTCATGGAGCCTTCATAGACTCTTGCTATGCTCACTGCCAAATTGGAACACAAGAGACATGGTTGGCGGCTGACTCCCCGGTTGTCGGCAAGACGACAATTGCGAAGGCAGTCGGAGACTGGTATTACAACCGAAGTCCATCAAAAAAGATTGATTGTCCTTACCCTTGCAATCCTACCTGCAAAAACAAGGAATTTGGTTCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

395

Amino Acids

42.54

Weight (kDa)

8.82

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 77 - 443 2.8e-175 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 910
Acc36I ACCTGC 3 cut(s) 715, 910, 1163
Acc65I GGTACC 1 cut(s) 763
AccB1I GGYRCC 2 cut(s) 763, 835
AccB7I CCANNNNNTGG 2 cut(s) 79, 980
AccBSI CCGCTC 1 cut(s) 244
AccIII TCCGGA 1 cut(s) 774
AciI CCGC 4 cut(s) 244, 576, 602, 1043
AclWI GGATC 2 cut(s) 649, 845
AcsI RAATTY 2 cut(s) 909, 1169
AcuI CTGAAG 2 cut(s) 241, 415
AfaI GTAC 3 cut(s) 368, 765, 951
AfeI AGCGCT 1 cut(s) 844
AfiI CCNNNNNNNGG 3 cut(s) 79, 980, 1061
AgeI ACCGGT 1 cut(s) 389
AgsI TTSAA 7 cut(s) 328, 548, 656, 723, 730, 914, 1179
AjnI CCWGG 1 cut(s) 733
AluBI AGCT 7 cut(s) 65, 119, 416, 524, 659, 873, 1182
AluI AGCT 7 cut(s) 65, 119, 416, 524, 659, 873, 1182
Alw26I GTCTC 3 cut(s) 968, 1025, 1086
AlwI GGATC 2 cut(s) 649, 845
Aor13HI TCCGGA 1 cut(s) 774
Aor51HI AGCGCT 1 cut(s) 844
AoxI GGCC 2 cut(s) 3, 19
ApeKI GCWGC 3 cut(s) 800, 845, 873
ApoI RAATTY 2 cut(s) 909, 1169
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AsiGI ACCGGT 1 cut(s) 389
Asp718I GGTACC 1 cut(s) 763
AspLEI GCGC 1 cut(s) 845
AspS9I GGNCC 2 cut(s) 94, 777
AsuC2I CCSGG 1 cut(s) 1055
AsuHPI GGTGA 4 cut(s) 379, 404, 891, 958
AvaII GGWCC 2 cut(s) 94, 777
BanI GGYRCC 2 cut(s) 763, 835
BbsI GAAGAC 1 cut(s) 290
BbvI GCAGC 3 cut(s) 787, 832, 860
BccI CCATC 7 cut(s) 81, 125, 207, 368, 874, 982, 1123
BciT130I CCWGG 1 cut(s) 735
BcnI CCSGG 1 cut(s) 1055
BcoDI GTCTC 3 cut(s) 968, 1025, 1086
BfaI CTAG 1 cut(s) 1183
BfoI RGCGCY 1 cut(s) 846
BfuAI ACCTGC 3 cut(s) 715, 910, 1163
BisI GCNGC 4 cut(s) 801, 846, 874, 1044
BlsI GCNGC 4 cut(s) 802, 847, 875, 1045
Bme1390I CCNGG 2 cut(s) 735, 1055
Bme18I GGWCC 2 cut(s) 94, 777
BmgT120I GGNCC 2 cut(s) 94, 777
BmiI GGNNCC 4 cut(s) 765, 779, 837, 985
BmrFI CCNGG 2 cut(s) 735, 1055
BmsI GCATC 2 cut(s) 595, 719
BpiI GAAGAC 1 cut(s) 290
BpmI CTGGAG 1 cut(s) 546
BpuMI CCSGG 1 cut(s) 1055
BsaI GGTCTC 1 cut(s) 968
BsaJI CCNNGG 1 cut(s) 1053
BsaWI WCCGGW 2 cut(s) 389, 774
BsaXI ACNNNNNCTCC 4 cut(s) 429, 459, 756, 786
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 980, 1061
Bse118I RCCGGY 2 cut(s) 142, 389
Bse1I ACTGG 3 cut(s) 22, 350, 1100
BseAI TCCGGA 1 cut(s) 774
BseBI CCWGG 1 cut(s) 735
BseDI CCNNGG 1 cut(s) 1053
BseGI GGATG 5 cut(s) 73, 218, 229, 266, 610
BseLI CCNNNNNNNGG 3 cut(s) 79, 980, 1061
BseNI ACTGG 3 cut(s) 22, 350, 1100
BseRI GAGGAG 2 cut(s) 222, 450
BseXI GCAGC 3 cut(s) 787, 832, 860
BseYI CCCAGC 1 cut(s) 412
BsgI GTGCAG 3 cut(s) 123, 192, 888
BshFI GGCC 2 cut(s) 5, 21
BshNI GGYRCC 2 cut(s) 763, 835
BshTI ACCGGT 1 cut(s) 389
BsiSI CCGG 4 cut(s) 143, 390, 775, 1055
BslI CCNNNNNNNGG 3 cut(s) 79, 980, 1061
BsmAI GTCTC 3 cut(s) 968, 1025, 1086
BsmI GAATGC 1 cut(s) 496
BsnI GGCC 2 cut(s) 5, 21
Bso31I GGTCTC 1 cut(s) 968
Bsp13I TCCGGA 1 cut(s) 774
Bsp143I GATC 3 cut(s) 466, 641, 850
BspACI CCGC 4 cut(s) 244, 576, 602, 1043
BspANI GGCC 2 cut(s) 5, 21
BspEI TCCGGA 1 cut(s) 774
BspLI GGNNCC 4 cut(s) 765, 779, 837, 985
BspMI ACCTGC 3 cut(s) 715, 910, 1163
BspPI GGATC 2 cut(s) 649, 845
BspT107I GGYRCC 2 cut(s) 763, 835
BspTNI GGTCTC 1 cut(s) 968
BsrBI CCGCTC 1 cut(s) 244
BsrFI RCCGGY 2 cut(s) 142, 389
BsrI ACTGG 3 cut(s) 22, 350, 1100
BssAI RCCGGY 2 cut(s) 142, 389
BssECI CCNNGG 1 cut(s) 1053
BssMI GATC 3 cut(s) 466, 641, 850
Bst2UI CCWGG 1 cut(s) 735
Bst4CI ACNGT 2 cut(s) 371, 668
Bst6I CTCTTC 1 cut(s) 623
BstC8I GCNNGC 1 cut(s) 576
BstDEI CTNAG 1 cut(s) 472
BstF5I GGATG 5 cut(s) 73, 218, 229, 266, 610
BstH2I RGCGCY 1 cut(s) 846
BstHHI GCGC 1 cut(s) 845
BstKTI GATC 3 cut(s) 469, 644, 853
BstMAI GTCTC 3 cut(s) 968, 1025, 1086
BstMBI GATC 3 cut(s) 466, 641, 850
BstMWI GCNNNNNNNGC 3 cut(s) 116, 500, 842
BstNI CCWGG 1 cut(s) 735
BstSCI CCNGG 2 cut(s) 733, 1053
BstV1I GCAGC 3 cut(s) 787, 832, 860
BstV2I GAAGAC 1 cut(s) 290
BstXI CCANNNNNNTGG 1 cut(s) 277
BsuRI GGCC 2 cut(s) 5, 21
BtsCI GGATG 5 cut(s) 73, 218, 229, 266, 610
BtsI GCAGTG 1 cut(s) 1009
BtsIMutI CAGTG 2 cut(s) 29, 1009
BveI ACCTGC 3 cut(s) 715, 910, 1163
Cac8I GCNNGC 1 cut(s) 576
CfoI GCGC 1 cut(s) 845
Cfr10I RCCGGY 2 cut(s) 142, 389
Cfr13I GGNCC 2 cut(s) 94, 777
Csp6I GTAC 3 cut(s) 367, 764, 950
CspAI ACCGGT 1 cut(s) 389
CviAII CATG 6 cut(s) 686, 714, 864, 919, 980, 1035
CviQI GTAC 3 cut(s) 367, 764, 950
DdeI CTNAG 1 cut(s) 472
DpnI GATC 3 cut(s) 468, 643, 852
DpnII GATC 3 cut(s) 466, 641, 850
Eam1104I CTCTTC 1 cut(s) 623
EarI CTCTTC 1 cut(s) 623
Eco31I GGTCTC 1 cut(s) 968
Eco47I GGWCC 2 cut(s) 94, 777
Eco47III AGCGCT 1 cut(s) 844
Eco57I CTGAAG 2 cut(s) 241, 415
EcoRII CCWGG 1 cut(s) 733
FaeI CATG 6 cut(s) 689, 717, 867, 922, 983, 1038
FatI CATG 6 cut(s) 685, 713, 863, 918, 979, 1034
FauI CCCGC 1 cut(s) 583
FauNDI CATATG 1 cut(s) 276
Fnu4HI GCNGC 4 cut(s) 801, 846, 874, 1044
FokI GGATG 5 cut(s) 60, 225, 236, 253, 617
Fsp4HI GCNGC 4 cut(s) 801, 846, 874, 1044
FspBI CTAG 1 cut(s) 1183
GlaI GCGC 1 cut(s) 844
GluI GCNGC 4 cut(s) 801, 846, 874, 1044
GsaI CCCAGC 1 cut(s) 416
GsuI CTGGAG 1 cut(s) 546
HaeII RGCGCY 1 cut(s) 846
HaeIII GGCC 2 cut(s) 5, 21
HapII CCGG 4 cut(s) 143, 390, 775, 1055
HhaI GCGC 1 cut(s) 845
Hin1II CATG 6 cut(s) 689, 717, 867, 922, 983, 1038
Hin6I GCGC 1 cut(s) 843
HinP1I GCGC 1 cut(s) 843
HindIII AAGCTT 1 cut(s) 657
HinfI GANTC 7 cut(s) 136, 328, 354, 730, 809, 995, 1049
HpaII CCGG 4 cut(s) 143, 390, 775, 1055
HphI GGTGA 4 cut(s) 379, 404, 891, 958
Hpy166II GTNNAC 3 cut(s) 47, 140, 409
Hpy188I TCNGA 3 cut(s) 260, 434, 1091
Hpy188III TCNNGA 5 cut(s) 97, 332, 639, 723, 775
Hpy8I GTNNAC 3 cut(s) 47, 140, 409
HpyAV CCTTC 3 cut(s) 435, 997, 1075
HpyCH4III ACNGT 2 cut(s) 371, 668
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 500, 842
HpyF3I CTNAG 1 cut(s) 472
Hsp92II CATG 6 cut(s) 689, 717, 867, 922, 983, 1038
HspAI GCGC 1 cut(s) 843
Kpn2I TCCGGA 1 cut(s) 774
KpnI GGTACC 1 cut(s) 767
Kzo9I GATC 3 cut(s) 466, 641, 850
LmnI GCTCC 2 cut(s) 116, 983
Lsp1109I GCAGC 3 cut(s) 787, 832, 860
LweI GCATC 2 cut(s) 595, 719
MaeI CTAG 1 cut(s) 1183
MaeIII GTNAC 1 cut(s) 611
MalI GATC 3 cut(s) 468, 643, 852
MbiI CCGCTC 1 cut(s) 244
MboI GATC 3 cut(s) 466, 641, 850
MboII GAAGA 5 cut(s) 295, 416, 536, 640, 739
MfeI CAATTG 2 cut(s) 184, 1074
MluCI AATT 6 cut(s) 184, 651, 909, 1017, 1074, 1169
MlyI GAGTC 3 cut(s) 145, 989, 1043
MmeI TCCRAC 1 cut(s) 1069
MroI TCCGGA 1 cut(s) 774
MseI TTAA 3 cut(s) 29, 180, 944
MspA1I CMGCKG 2 cut(s) 524, 578
MspI CCGG 4 cut(s) 143, 390, 775, 1055
MspR9I CCNGG 2 cut(s) 735, 1055
MunI CAATTG 2 cut(s) 184, 1074
Mva1269I GAATGC 1 cut(s) 496
MvaI CCWGG 1 cut(s) 735
MwoI GCNNNNNNNGC 3 cut(s) 116, 500, 842
NciI CCSGG 1 cut(s) 1055
NdeI CATATG 1 cut(s) 276
NdeII GATC 3 cut(s) 466, 641, 850
NlaIII CATG 6 cut(s) 689, 717, 867, 922, 983, 1038
NlaIV GGNNCC 4 cut(s) 765, 779, 837, 985
PaqCI CACCTGC 1 cut(s) 910
PcsI WCGNNNNNNNCGW 1 cut(s) 1076
PctI GAATGC 1 cut(s) 496
PfeI GAWTC 4 cut(s) 328, 354, 730, 809
PflMI CCANNNNNTGG 2 cut(s) 79, 980
PfoI TCCNGGA 1 cut(s) 733
PinAI ACCGGT 1 cut(s) 389
PkrI GCNGC 4 cut(s) 802, 847, 875, 1045
PleI GAGTC 3 cut(s) 144, 989, 1043
PpsI GAGTC 3 cut(s) 144, 989, 1043
Psp6I CCWGG 1 cut(s) 733
PspFI CCCAGC 1 cut(s) 412
PspGI CCWGG 1 cut(s) 733
PspN4I GGNNCC 4 cut(s) 765, 779, 837, 985
PspPI GGNCC 2 cut(s) 94, 777
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 3 cut(s) 368, 765, 951
RsaNI GTAC 3 cut(s) 367, 764, 950
SaqAI TTAA 3 cut(s) 29, 180, 944
SatI GCNGC 4 cut(s) 801, 846, 874, 1044
Sau3AI GATC 3 cut(s) 466, 641, 850
Sau96I GGNCC 2 cut(s) 94, 777
SchI GAGTC 3 cut(s) 145, 989, 1043
ScrFI CCNGG 2 cut(s) 735, 1055
SfaNI GCATC 2 cut(s) 595, 719
SgrAI CRCCGGYG 1 cut(s) 389
SinI GGWCC 2 cut(s) 94, 777
Sse9I AATT 6 cut(s) 184, 651, 909, 1017, 1074, 1169
SsiI CCGC 4 cut(s) 244, 576, 602, 1043
SspI AATATT 1 cut(s) 829
SspMI CTAG 1 cut(s) 1183
StyD4I CCNGG 2 cut(s) 733, 1053
TaaI ACNGT 2 cut(s) 371, 668
TasI AATT 6 cut(s) 184, 651, 909, 1017, 1074, 1169
TatI WGTACW 1 cut(s) 949
TauI GCSGC 1 cut(s) 1046
TfiI GAWTC 4 cut(s) 328, 354, 730, 809
Tru1I TTAA 3 cut(s) 29, 180, 944
Tru9I TTAA 3 cut(s) 29, 180, 944
TscAI CASTG 2 cut(s) 29, 1016
TseI GCWGC 3 cut(s) 800, 845, 873
TspDTI ATGAA 5 cut(s) 185, 255, 503, 607, 979
TspGWI ACGGA 1 cut(s) 551
TspRI CASTG 2 cut(s) 29, 1016
Van91I CCANNNNNTGG 2 cut(s) 79, 980
VpaK11BI GGWCC 2 cut(s) 94, 777
XapI RAATTY 2 cut(s) 909, 1169
XspI CTAG 1 cut(s) 1183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.