Rmu_ssc0000134.1_g000040

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000134.1
OG0000996
Physical Location & Seq
Reverse (-)
147501 .. 149406
1906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000134.1_g000040.1.cds

Sequence Viewer

Length: 921 bp
atgctgaaggaaattcctttttctggaattctaagcaacagggaatcacttaatccaggcttttacaattggaatagaatcaaagtcaggtactgcgatgggtcatcattcactggagatgttgaagcagtacacccagctactggtctttactttagaggagctagggtttttcgagccgttattgctgatttattagcaaaaggaatggggaaagctgaaaatgctgtcctctccggttgttctgctggtggattggcctccattcttcattgtgataacttccgtgctctcttaccttccggaaccattgtgaaatgtgtttcagatgctggttactttattaatgtaaaggacatttctggagcacaacacattcaacagtattttgctgatgttgttacaacacatggttctgcgaagaatttgcctccatattgcacttcaaaattgagaccagatttgtgtttcttccctcaatatgtggtaccacagattcggacaccaatattctatgtacattcagcctatgattcatggcagataaggaacattttggtaccaggtgttgcggatcctcatggaacttggcgaaactgcaagctcaatataaagaactgctcacccgctcagttcaaaaccattgaaagcttcaggttgcaatttttaactgcaattggtggtgtgagcaactctgcagtaaagggaatgttcatagactcttgctatctacactgtcaaactgaaatgcaggagacttggttaatgcctaattctccactccttaacaaaatgacaattgcaggggcagttagagactggttttatgaccgaactccattccaaaagatagactgtccttactcttgcaatcccacttgccatcctccgatgtttgatccaaatgtgcacttggaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

306

Amino Acids

34.11

Weight (kDa)

8.52

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 487, 559
AccB1I GGYRCC 2 cut(s) 487, 559
AccB7I CCANNNNNTGG 1 cut(s) 143
AccBSI CCGCTC 1 cut(s) 629
AccIII TCCGGA 1 cut(s) 302
AciI CCGC 2 cut(s) 572, 627
AclWI GGATC 3 cut(s) 569, 582, 893
AcsI RAATTY 3 cut(s) 12, 27, 424
AcuI CTGAAG 2 cut(s) 26, 637
AfaI GTAC 5 cut(s) 92, 132, 489, 519, 561
AfiI CCNNNNNNNGG 2 cut(s) 23, 143
AgsI TTSAA 5 cut(s) 125, 380, 447, 637, 647
AjnI CCWGG 2 cut(s) 55, 562
AluBI AGCT 5 cut(s) 140, 164, 218, 604, 651
AluI AGCT 5 cut(s) 140, 164, 218, 604, 651
Alw21I GWGCWC 3 cut(s) 292, 370, 912
Alw26I GTCTC 3 cut(s) 448, 749, 810
Alw44I GTGCAC 1 cut(s) 908
AlwI GGATC 3 cut(s) 569, 582, 893
AlwNI CAGNNNCTG 3 cut(s) 93, 143, 332
Aor13HI TCCGGA 1 cut(s) 302
AoxI GGCC 1 cut(s) 258
ApaLI GTGCAC 1 cut(s) 908
ApoI RAATTY 3 cut(s) 12, 27, 424
AseI ATTAAT 1 cut(s) 345
Asp718I GGTACC 2 cut(s) 487, 559
AsuHPI GGTGA 1 cut(s) 615
BaeGI GKGCMC 1 cut(s) 912
BamHI GGATCC 1 cut(s) 574
BanI GGYRCC 2 cut(s) 487, 559
Bbv12I GWGCWC 3 cut(s) 292, 370, 912
BccI CCATC 2 cut(s) 92, 891
BceAI ACGGC 1 cut(s) 164
BcgI CGANNNNNNTGC 2 cut(s) 409, 443
BciT130I CCWGG 2 cut(s) 57, 564
BcoDI GTCTC 3 cut(s) 448, 749, 810
BfaI CTAG 1 cut(s) 165
BfmI CTRYAG 1 cut(s) 696
Bme1390I CCNGG 2 cut(s) 57, 564
BmiI GGNNCC 4 cut(s) 307, 489, 561, 576
BmrFI CCNGG 2 cut(s) 57, 564
BmsI GCATC 1 cut(s) 319
BpmI CTGGAG 2 cut(s) 135, 384
BsaI GGTCTC 1 cut(s) 448
BsaWI WCCGGW 2 cut(s) 236, 302
BsaXI ACNNNNNCTCC 4 cut(s) 153, 183, 746, 776
Bsc4I CCNNNNNNNGG 2 cut(s) 23, 143
Bse1I ACTGG 3 cut(s) 118, 148, 824
BseAI TCCGGA 1 cut(s) 302
BseBI CCWGG 2 cut(s) 57, 564
BseGI GGATG 1 cut(s) 883
BseLI CCNNNNNNNGG 2 cut(s) 23, 143
BseMII CTCAG 1 cut(s) 644
BseNI ACTGG 3 cut(s) 118, 148, 824
BseRI GAGGAG 1 cut(s) 174
BseSI GKGCMC 1 cut(s) 912
BseYI CCCAGC 1 cut(s) 136
BshFI GGCC 1 cut(s) 260
BshNI GGYRCC 2 cut(s) 487, 559
BsiHKAI GWGCWC 3 cut(s) 292, 370, 912
BsiSI CCGG 2 cut(s) 237, 303
BslI CCNNNNNNNGG 2 cut(s) 23, 143
BsmAI GTCTC 3 cut(s) 448, 749, 810
BsnI GGCC 1 cut(s) 260
Bso31I GGTCTC 1 cut(s) 448
Bsp1286I GDGCHC 3 cut(s) 292, 370, 912
Bsp13I TCCGGA 1 cut(s) 302
Bsp1407I TGTACA 1 cut(s) 517
Bsp143I GATC 2 cut(s) 574, 898
BspACI CCGC 2 cut(s) 572, 627
BspANI GGCC 1 cut(s) 260
BspCNI CTCAG 1 cut(s) 643
BspEI TCCGGA 1 cut(s) 302
BspLI GGNNCC 4 cut(s) 307, 489, 561, 576
BspMAI CTGCAG 1 cut(s) 700
BspPI GGATC 3 cut(s) 569, 582, 893
BspT107I GGYRCC 2 cut(s) 487, 559
BspTNI GGTCTC 1 cut(s) 448
BsrBI CCGCTC 1 cut(s) 629
BsrGI TGTACA 1 cut(s) 517
BsrI ACTGG 3 cut(s) 118, 148, 824
BssMI GATC 2 cut(s) 574, 898
Bst2UI CCWGG 2 cut(s) 57, 564
Bst4CI ACNGT 3 cut(s) 384, 737, 857
BstAUI TGTACA 1 cut(s) 517
BstC8I GCNNGC 1 cut(s) 602
BstDEI CTNAG 2 cut(s) 32, 630
BstF5I GGATG 1 cut(s) 883
BstKTI GATC 2 cut(s) 577, 901
BstMAI GTCTC 3 cut(s) 448, 749, 810
BstMBI GATC 2 cut(s) 574, 898
BstMWI GCNNNNNNNGC 2 cut(s) 185, 224
BstNI CCWGG 2 cut(s) 57, 564
BstSCI CCNGG 2 cut(s) 55, 562
BstSFI CTRYAG 1 cut(s) 696
BstSLI GKGCMC 1 cut(s) 912
BstX2I RGATCY 1 cut(s) 574
BstYI RGATCY 1 cut(s) 574
BsuRI GGCC 1 cut(s) 260
BtgZI GCGATG 1 cut(s) 111
BtsCI GGATG 1 cut(s) 883
BtsIMutI CAGTG 2 cut(s) 111, 733
Cac8I GCNNGC 1 cut(s) 602
CaiI CAGNNNCTG 3 cut(s) 93, 143, 332
CsiI ACCWGGT 1 cut(s) 562
Csp6I GTAC 5 cut(s) 91, 131, 488, 518, 560
CviAII CATG 3 cut(s) 410, 537, 581
CviJI RGCY 9 cut(s) 60, 140, 164, 179, 218, 260, 527, 604, 651
CviKI_1 RGCY 9 cut(s) 60, 140, 164, 179, 218, 260, 527, 604, 651
CviQI GTAC 5 cut(s) 91, 131, 488, 518, 560
DdeI CTNAG 2 cut(s) 32, 630
DpnI GATC 2 cut(s) 576, 900
DpnII GATC 2 cut(s) 574, 898
Eco31I GGTCTC 1 cut(s) 448
Eco57I CTGAAG 2 cut(s) 26, 637
EcoRI GAATTC 1 cut(s) 27
EcoRII CCWGG 2 cut(s) 55, 562
FaeI CATG 3 cut(s) 413, 540, 584
FatI CATG 3 cut(s) 409, 536, 580
FauI CCCGC 1 cut(s) 634
FokI GGATG 1 cut(s) 870
FspBI CTAG 1 cut(s) 165
GsaI CCCAGC 1 cut(s) 140
GsuI CTGGAG 2 cut(s) 135, 384
HaeIII GGCC 1 cut(s) 260
HapII CCGG 2 cut(s) 237, 303
Hin1II CATG 3 cut(s) 413, 540, 584
HindIII AAGCTT 1 cut(s) 649
HinfI GANTC 5 cut(s) 44, 78, 496, 533, 719
HpaII CCGG 2 cut(s) 237, 303
HphI GGTGA 1 cut(s) 615
Hpy166II GTNNAC 2 cut(s) 133, 910
Hpy188I TCNGA 3 cut(s) 328, 501, 891
Hpy188III TCNNGA 3 cut(s) 24, 303, 363
Hpy8I GTNNAC 2 cut(s) 133, 910
HpyAV CCTTC 1 cut(s) 309
HpyCH4III ACNGT 3 cut(s) 384, 737, 857
HpyCH4V TGCA 9 cut(s) 441, 600, 661, 674, 698, 751, 803, 870, 910
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 224
HpyF3I CTNAG 2 cut(s) 32, 630
Hsp92II CATG 3 cut(s) 413, 540, 584
Kpn2I TCCGGA 1 cut(s) 302
KpnI GGTACC 2 cut(s) 491, 563
Kzo9I GATC 2 cut(s) 574, 898
LmnI GCTCC 2 cut(s) 161, 365
LweI GCATC 1 cut(s) 319
MabI ACCWGGT 1 cut(s) 562
MaeI CTAG 1 cut(s) 165
MaeIII GTNAC 2 cut(s) 335, 400
MalI GATC 2 cut(s) 576, 900
MbiI CCGCTC 1 cut(s) 629
MboI GATC 2 cut(s) 574, 898
MboII GAAGA 3 cut(s) 260, 433, 463
MfeI CAATTG 3 cut(s) 67, 675, 798
MflI RGATCY 1 cut(s) 574
MhlI GDGCHC 3 cut(s) 292, 370, 912
MluCI AATT 9 cut(s) 12, 27, 67, 424, 449, 662, 675, 772, 798
MlyI GAGTC 1 cut(s) 713
MnlI CCTC 7 cut(s) 152, 242, 271, 441, 486, 588, 897
MroI TCCGGA 1 cut(s) 302
MseI TTAA 5 cut(s) 51, 345, 668, 764, 786
MspI CCGG 2 cut(s) 237, 303
MspR9I CCNGG 2 cut(s) 57, 564
MunI CAATTG 3 cut(s) 67, 675, 798
MvaI CCWGG 2 cut(s) 57, 564
MwoI GCNNNNNNNGC 2 cut(s) 185, 224
NdeII GATC 2 cut(s) 574, 898
NlaIII CATG 3 cut(s) 413, 540, 584
NlaIV GGNNCC 4 cut(s) 307, 489, 561, 576
PfeI GAWTC 4 cut(s) 44, 78, 496, 533
PflMI CCANNNNNTGG 1 cut(s) 143
PleI GAGTC 1 cut(s) 713
PpsI GAGTC 1 cut(s) 713
PshBI ATTAAT 1 cut(s) 345
Psp6I CCWGG 2 cut(s) 55, 562
PspFI CCCAGC 1 cut(s) 136
PspGI CCWGG 2 cut(s) 55, 562
PspN4I GGNNCC 4 cut(s) 307, 489, 561, 576
PstI CTGCAG 1 cut(s) 700
PstNI CAGNNNCTG 3 cut(s) 93, 143, 332
PsuI RGATCY 1 cut(s) 574
RsaI GTAC 5 cut(s) 92, 132, 489, 519, 561
RsaNI GTAC 5 cut(s) 91, 131, 488, 518, 560
SaqAI TTAA 5 cut(s) 51, 345, 668, 764, 786
Sau3AI GATC 2 cut(s) 574, 898
SchI GAGTC 1 cut(s) 713
ScrFI CCNGG 2 cut(s) 57, 564
SduI GDGCHC 3 cut(s) 292, 370, 912
SetI ASST 9 cut(s) 92, 142, 166, 220, 301, 568, 606, 653, 659
SexAI ACCWGGT 1 cut(s) 562
SfaNI GCATC 1 cut(s) 319
SfcI CTRYAG 1 cut(s) 696
Sse9I AATT 9 cut(s) 12, 27, 67, 424, 449, 662, 675, 772, 798
SsiI CCGC 2 cut(s) 572, 627
SspI AATATT 1 cut(s) 510
SspMI CTAG 1 cut(s) 165
StyD4I CCNGG 2 cut(s) 55, 562
TaaI ACNGT 3 cut(s) 384, 737, 857
TaqI TCGA 1 cut(s) 175
TaqII GACCGA 1 cut(s) 846
TasI AATT 9 cut(s) 12, 27, 67, 424, 449, 662, 675, 772, 798
TatI WGTACW 2 cut(s) 130, 517
TfiI GAWTC 4 cut(s) 44, 78, 496, 533
Tru1I TTAA 5 cut(s) 51, 345, 668, 764, 786
Tru9I TTAA 5 cut(s) 51, 345, 668, 764, 786
TscAI CASTG 2 cut(s) 118, 740
TspDTI ATGAA 3 cut(s) 260, 525, 703
TspGWI ACGGA 1 cut(s) 275
TspRI CASTG 2 cut(s) 118, 740
Van91I CCANNNNNTGG 1 cut(s) 143
VneI GTGCAC 1 cut(s) 908
VspI ATTAAT 1 cut(s) 345
XapI RAATTY 3 cut(s) 12, 27, 424
XspI CTAG 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.