MD09G1232400.v1.1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000996
Physical Location & Seq
Forward (+)
28990099 .. 28991828
1730 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1232400.v1.1.491

Sequence Viewer

Length: 471 bp
ATGAGTCCAGGGCTGTGCTTCTTCCCACAAAATGTGGTACCCCAAACCCGGACACCAATATTTTTTGTCAATGCAGCTTATGATTCCTGGCAGATAAAGAACATTTTGGCACCGGGTGTTGCTGATCCTAAAGGTGCCTGGAAAAATTGCAAGCTTAGTATAAAGAACTGCTCACCCAGTCAACTTCAAACCATGCAAGAATATAGGTTACAGTTCTTAAACGCAGTTGCTGGTGCGAGCAGTTCTTCATCTAACGGACTGTTCATCGACTCCTGCTACGCTCATTGCCAAATCGGAACACAGGAGACATGGCTGGCAACTGATTCTCCCATGTTGAGTAAGACGACAATTGCAAAGGCAGTTGGAGACTGGTATTACGACCGCACTCCGTTTAAAAATATTGATTGCCCTTACCCTTGCAATCCCACTTGCAAAAACCGTGAATTTGATTCAAATGATCAGCAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

157

Amino Acids

17.42

Weight (kDa)

6.53

Isoelectric Point (pI)

54.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 1 - 145 2.8e-61 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 37
AccB1I GGYRCC 3 cut(s) 37, 109, 134
AciI CCGC 1 cut(s) 382
AclWI GGATC 1 cut(s) 119
AcsI RAATTY 1 cut(s) 443
AdeI CACNNNGTG 1 cut(s) 116
AfaI GTAC 1 cut(s) 39
AfiI CCNNNNNNNGG 1 cut(s) 48
AgsI TTSAA 2 cut(s) 188, 453
AjnI CCWGG 3 cut(s) 7, 86, 137
AluBI AGCT 2 cut(s) 77, 154
AluI AGCT 2 cut(s) 77, 154
Alw26I GTCTC 2 cut(s) 299, 360
AlwI GGATC 1 cut(s) 119
AlwNI CAGNNNCTG 1 cut(s) 230
ApeKI GCWGC 1 cut(s) 74
ApoI RAATTY 1 cut(s) 443
Asp718I GGTACC 1 cut(s) 37
AsuC2I CCSGG 2 cut(s) 49, 114
AsuHPI GGTGA 1 cut(s) 165
BanI GGYRCC 3 cut(s) 37, 109, 134
BbvI GCAGC 1 cut(s) 86
BciT130I CCWGG 3 cut(s) 9, 88, 139
BclI TGATCA 1 cut(s) 457
BcnI CCSGG 2 cut(s) 49, 114
BcoDI GTCTC 2 cut(s) 299, 360
BisI GCNGC 1 cut(s) 75
BlsI GCNGC 1 cut(s) 76
Bme1390I CCNGG 5 cut(s) 9, 49, 88, 114, 139
BmiI GGNNCC 3 cut(s) 39, 111, 136
BmrFI CCNGG 5 cut(s) 9, 49, 88, 114, 139
BmrI ACTGGG 1 cut(s) 171
BmuI ACTGGG 1 cut(s) 171
BpuMI CCSGG 2 cut(s) 49, 114
BsaJI CCNNGG 1 cut(s) 8
BsaXI ACNNNNNCTCC 2 cut(s) 310, 340
Bsc4I CCNNNNNNNGG 1 cut(s) 48
Bse1I ACTGG 2 cut(s) 177, 374
Bse3DI GCAATG 1 cut(s) 283
BseBI CCWGG 3 cut(s) 9, 88, 139
BseDI CCNNGG 1 cut(s) 8
BseLI CCNNNNNNNGG 1 cut(s) 48
BseMI GCAATG 1 cut(s) 283
BseNI ACTGG 2 cut(s) 177, 374
BseXI GCAGC 1 cut(s) 86
Bsh1285I CGRYCG 1 cut(s) 382
BshNI GGYRCC 3 cut(s) 37, 109, 134
BsiEI CGRYCG 1 cut(s) 382
BsiSI CCGG 2 cut(s) 49, 113
BslI CCNNNNNNNGG 1 cut(s) 48
BsmAI GTCTC 2 cut(s) 299, 360
Bsp143I GATC 2 cut(s) 124, 457
BspACI CCGC 1 cut(s) 382
BspLI GGNNCC 3 cut(s) 39, 111, 136
BspPI GGATC 1 cut(s) 119
BspT107I GGYRCC 3 cut(s) 37, 109, 134
BsrDI GCAATG 1 cut(s) 283
BsrI ACTGG 2 cut(s) 177, 374
BssECI CCNNGG 1 cut(s) 8
BssMI GATC 2 cut(s) 124, 457
Bst2UI CCWGG 3 cut(s) 9, 88, 139
Bst4CI ACNGT 3 cut(s) 213, 261, 440
BstC8I GCNNGC 3 cut(s) 152, 238, 315
BstDEI CTNAG 1 cut(s) 155
BstKTI GATC 2 cut(s) 127, 460
BstMAI GTCTC 2 cut(s) 299, 360
BstMBI GATC 2 cut(s) 124, 457
BstMCI CGRYCG 1 cut(s) 382
BstNI CCWGG 3 cut(s) 9, 88, 139
BstSCI CCNGG 5 cut(s) 7, 47, 86, 112, 137
BstV1I GCAGC 1 cut(s) 86
Cac8I GCNNGC 3 cut(s) 152, 238, 315
CaiI CAGNNNCTG 1 cut(s) 230
Csp6I GTAC 1 cut(s) 38
CviAII CATG 3 cut(s) 193, 309, 331
CviJI RGCY 4 cut(s) 13, 77, 154, 313
CviKI_1 RGCY 4 cut(s) 13, 77, 154, 313
CviQI GTAC 1 cut(s) 38
DdeI CTNAG 1 cut(s) 155
DpnI GATC 2 cut(s) 126, 459
DpnII GATC 2 cut(s) 124, 457
DraI TTTAAA 1 cut(s) 394
DraIII CACNNNGTG 1 cut(s) 116
EcoRII CCWGG 3 cut(s) 7, 86, 137
FaeI CATG 3 cut(s) 196, 312, 334
FaiI YATR 6 cut(s) 81, 161, 194, 204, 310, 332
FatI CATG 3 cut(s) 192, 308, 330
FbaI TGATCA 1 cut(s) 457
Fnu4HI GCNGC 1 cut(s) 75
Fsp4HI GCNGC 1 cut(s) 75
GluI GCNGC 1 cut(s) 75
HapII CCGG 2 cut(s) 49, 113
Hin1II CATG 3 cut(s) 196, 312, 334
HincII GTYRAC 1 cut(s) 182
HindII GTYRAC 1 cut(s) 182
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 5 cut(s) 4, 83, 269, 323, 449
HpaII CCGG 2 cut(s) 49, 113
HphI GGTGA 1 cut(s) 165
Hpy166II GTNNAC 1 cut(s) 182
Hpy188I TCNGA 1 cut(s) 296
Hpy8I GTNNAC 1 cut(s) 182
HpyCH4III ACNGT 3 cut(s) 213, 261, 440
HpyCH4V TGCA 6 cut(s) 74, 150, 196, 353, 420, 432
HpyF3I CTNAG 1 cut(s) 155
Hsp92II CATG 3 cut(s) 196, 312, 334
KpnI GGTACC 1 cut(s) 41
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 2 cut(s) 124, 457
Lsp1109I GCAGC 1 cut(s) 86
MaeIII GTNAC 1 cut(s) 207
MalI GATC 2 cut(s) 126, 459
MboI GATC 2 cut(s) 124, 457
MboII GAAGA 2 cut(s) 13, 237
MfeI CAATTG 1 cut(s) 348
MluCI AATT 3 cut(s) 145, 348, 443
MlyI GAGTC 2 cut(s) 13, 263
MmeI TCCRAC 1 cut(s) 343
MseI TTAA 3 cut(s) 218, 393, 469
MspI CCGG 2 cut(s) 49, 113
MspR9I CCNGG 5 cut(s) 9, 49, 88, 114, 139
MunI CAATTG 1 cut(s) 348
MvaI CCWGG 3 cut(s) 9, 88, 139
NciI CCSGG 2 cut(s) 49, 114
NdeII GATC 2 cut(s) 124, 457
NlaIII CATG 3 cut(s) 196, 312, 334
NlaIV GGNNCC 3 cut(s) 39, 111, 136
PfeI GAWTC 3 cut(s) 83, 323, 449
PkrI GCNGC 1 cut(s) 76
PleI GAGTC 2 cut(s) 12, 263
PpsI GAGTC 2 cut(s) 12, 263
Psp6I CCWGG 3 cut(s) 7, 86, 137
PspGI CCWGG 3 cut(s) 7, 86, 137
PspN4I GGNNCC 3 cut(s) 39, 111, 136
PstNI CAGNNNCTG 1 cut(s) 230
RsaI GTAC 1 cut(s) 39
RsaNI GTAC 1 cut(s) 38
SaqAI TTAA 3 cut(s) 218, 393, 469
SatI GCNGC 1 cut(s) 75
Sau3AI GATC 2 cut(s) 124, 457
SchI GAGTC 2 cut(s) 13, 263
ScrFI CCNGG 5 cut(s) 9, 49, 88, 114, 139
SetI ASST 4 cut(s) 79, 136, 156, 209
Sse9I AATT 3 cut(s) 145, 348, 443
SsiI CCGC 1 cut(s) 382
SspI AATATT 2 cut(s) 60, 400
StyD4I CCNGG 5 cut(s) 7, 47, 86, 112, 137
TaaI ACNGT 3 cut(s) 213, 261, 440
TaqI TCGA 1 cut(s) 267
TasI AATT 3 cut(s) 145, 348, 443
TfiI GAWTC 3 cut(s) 83, 323, 449
Tru1I TTAA 3 cut(s) 218, 393, 469
Tru9I TTAA 3 cut(s) 218, 393, 469
TseI GCWGC 1 cut(s) 74
TspDTI ATGAA 2 cut(s) 237, 253
TspGWI ACGGA 2 cut(s) 270, 378
XapI RAATTY 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.