Rh7DG289200

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000996
Physical Location & Seq
Reverse (-)
32422713 .. 32427316
4604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG289200.1

Sequence Viewer

Length: 963 bp
ATGTTTCATACTTATATTTGCAGAATGGCCTATGCAAGATTAGGCCAGTGGTTAAGTCTTCTGGTATGTTTACTGATATTGCTTAAAGCTGAAAGCCATCCTGTGGAACTCACCTTGGTCCAGAGTGCAGTTGCCAAAGGAGCTGTTTGTTTAGACGGGAGCCCACCAGCATACCACTTTGACAAGGGATTTGGTGCAGGGATTAACAATTGGTTGGTTCATATTGAGGGAGGAGGATGGTGTAAGGATGTTGAGAGTTGCGTTGAGCGAACGAAAAACTTCAGAGGTTCATCCACAAAAATGGAAAAGACAATATCTTTTTCTGGAGTTCTTGGTAAAAAGCAAACTATGAATCCAGACTTCTATAACTGGAACAGAATCAAGGTTAGGTACTGTGATGGGTCATCATTCACCGGTGATGTGGAAGCAGTAGACCCAGCTACAAATCTTCACTTCAGAGGAGGAAGGGTTTTTCGTGCCATCATTGATGATCTATTAGCAAAGGGAATGATAAATGCCAAAAATGCTCTACTCTCTGGTTGTTCGGCTGGAGGATTGGCTTCTATTCTTCAATGTGATAACTTCCGTTCTCTCTTACCCGCCGGAACTAATGTGAAATGCCTTGCGGATGCTGGTTACTTCATCAATGCGAAGACTATTACTGGGTCACAACAAATTGAAGCCTTTTATAGTCAAGTGGTTGCACTACATGGTTCAGCAAAGCATTTACCTGCATCCTGCACTTCAAGATTGAGACCAGGGTTGTGCTTCTTCCCACAAAATGTGATACCGCAACTCCGGACTCCTATTTTTTTGGTAAATGCTGCTTATGATTCGTGGCAGATAAAGAACATTTTGGCACCGAGTATTGCTGATCCTAAAGGCACATGGAAAAGCTGCAAGTCTGGTATAAAGAACTGCTCACCTGCGCAAATTCAAACCATGCAAGGTAAAAAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

320

Amino Acids

34.83

Weight (kDa)

9.26

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 29 - 317 4e-141 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 934
Acc16I TGCGCA 1 cut(s) 930
Acc36I ACCTGC 2 cut(s) 739, 934
AccB1I GGYRCC 1 cut(s) 859
AccB7I CCANNNNNTGG 1 cut(s) 103
AccI GTMKAC 1 cut(s) 432
AccIII TCCGGA 1 cut(s) 798
AciI CCGC 3 cut(s) 600, 626, 791
AclWI GGATC 1 cut(s) 869
AcsI RAATTY 1 cut(s) 933
AcuI CTGAAG 2 cut(s) 265, 439
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 1 cut(s) 103
AgeI ACCGGT 1 cut(s) 413
AgsI TTSAA 4 cut(s) 572, 680, 747, 938
AjnI CCWGG 1 cut(s) 757
AluBI AGCT 5 cut(s) 89, 143, 440, 897, 959
AluI AGCT 5 cut(s) 89, 143, 440, 897, 959
Alw26I GTCTC 1 cut(s) 748
AlwI GGATC 1 cut(s) 869
Aor13HI TCCGGA 1 cut(s) 798
AoxI GGCC 2 cut(s) 27, 43
ApeKI GCWGC 2 cut(s) 824, 897
ApoI RAATTY 1 cut(s) 933
ArsI GACNNNNNNTTYG 2 cut(s) 173, 205
AsiGI ACCGGT 1 cut(s) 413
Asp700I GAANNNNTTC 1 cut(s) 278
AspLEI GCGC 1 cut(s) 931
AspS9I GGNCC 1 cut(s) 118
AsuHPI GGTGA 4 cut(s) 103, 403, 428, 915
AvaII GGWCC 1 cut(s) 118
BanI GGYRCC 1 cut(s) 859
BanII GRGCYC 1 cut(s) 164
BbsI GAAGAC 2 cut(s) 50, 659
BbvI GCAGC 2 cut(s) 811, 884
BccI CCATC 4 cut(s) 105, 231, 392, 488
BciT130I CCWGG 1 cut(s) 759
BcoDI GTCTC 1 cut(s) 748
BfuAI ACCTGC 2 cut(s) 739, 934
BisI GCNGC 2 cut(s) 825, 898
BlsI GCNGC 2 cut(s) 826, 899
Bme1390I CCNGG 1 cut(s) 759
Bme18I GGWCC 1 cut(s) 118
BmgT120I GGNCC 1 cut(s) 118
BmiI GGNNCC 2 cut(s) 161, 861
BmrFI CCNGG 1 cut(s) 759
BmrI ACTGGG 1 cut(s) 672
BmsI GCATC 2 cut(s) 619, 743
BmuI ACTGGG 1 cut(s) 672
BpiI GAAGAC 2 cut(s) 50, 659
BpmI CTGGAG 2 cut(s) 345, 570
BsaI GGTCTC 1 cut(s) 748
BsaJI CCNNGG 2 cut(s) 114, 758
BsaWI WCCGGW 2 cut(s) 413, 798
BsaXI ACNNNNNCTCC 4 cut(s) 453, 483, 780, 810
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse118I RCCGGY 1 cut(s) 413
Bse1I ACTGG 3 cut(s) 46, 374, 667
BseAI TCCGGA 1 cut(s) 798
BseBI CCWGG 1 cut(s) 759
BseDI CCNNGG 2 cut(s) 114, 758
BseGI GGATG 6 cut(s) 97, 242, 253, 290, 634, 734
BseLI CCNNNNNNNGG 1 cut(s) 103
BseNI ACTGG 3 cut(s) 46, 374, 667
BseRI GAGGAG 2 cut(s) 246, 474
BseXI GCAGC 2 cut(s) 811, 884
BseYI CCCAGC 1 cut(s) 436
BsgI GTGCAG 3 cut(s) 147, 216, 724
BshFI GGCC 2 cut(s) 29, 45
BshNI GGYRCC 1 cut(s) 859
BshTI ACCGGT 1 cut(s) 413
BsiSI CCGG 3 cut(s) 414, 603, 799
BslI CCNNNNNNNGG 1 cut(s) 103
BsmAI GTCTC 1 cut(s) 748
BsnI GGCC 2 cut(s) 29, 45
Bso31I GGTCTC 1 cut(s) 748
Bsp1286I GDGCHC 1 cut(s) 164
Bsp13I TCCGGA 1 cut(s) 798
Bsp143I GATC 2 cut(s) 490, 874
BspACI CCGC 3 cut(s) 600, 626, 791
BspANI GGCC 2 cut(s) 29, 45
BspEI TCCGGA 1 cut(s) 798
BspLI GGNNCC 2 cut(s) 161, 861
BspMI ACCTGC 2 cut(s) 739, 934
BspPI GGATC 1 cut(s) 869
BspT107I GGYRCC 1 cut(s) 859
BspTNI GGTCTC 1 cut(s) 748
BsrFI RCCGGY 1 cut(s) 413
BsrI ACTGG 3 cut(s) 46, 374, 667
BssAI RCCGGY 1 cut(s) 413
BssECI CCNNGG 2 cut(s) 114, 758
BssMI GATC 2 cut(s) 490, 874
BssT1I CCWWGG 1 cut(s) 114
Bst2UI CCWGG 1 cut(s) 759
Bst4CI ACNGT 1 cut(s) 395
BstF5I GGATG 6 cut(s) 97, 242, 253, 290, 634, 734
BstHHI GCGC 1 cut(s) 931
BstKTI GATC 2 cut(s) 493, 877
BstMAI GTCTC 1 cut(s) 748
BstMBI GATC 2 cut(s) 490, 874
BstMWI GCNNNNNNNGC 2 cut(s) 140, 524
BstNI CCWGG 1 cut(s) 759
BstSCI CCNGG 1 cut(s) 757
BstV1I GCAGC 2 cut(s) 811, 884
BstV2I GAAGAC 2 cut(s) 50, 659
BstXI CCANNNNNNTGG 1 cut(s) 301
BsuRI GGCC 2 cut(s) 29, 45
BtsCI GGATG 6 cut(s) 97, 242, 253, 290, 634, 734
BtsIMutI CAGTG 1 cut(s) 53
BveI ACCTGC 2 cut(s) 739, 934
CfoI GCGC 1 cut(s) 931
Cfr10I RCCGGY 1 cut(s) 413
Cfr13I GGNCC 1 cut(s) 118
Csp6I GTAC 1 cut(s) 391
CspAI ACCGGT 1 cut(s) 413
CviAII CATG 3 cut(s) 710, 888, 943
CviQI GTAC 1 cut(s) 391
DpnI GATC 2 cut(s) 492, 876
DpnII GATC 2 cut(s) 490, 874
Eco130I CCWWGG 1 cut(s) 114
Eco24I GRGCYC 1 cut(s) 164
Eco31I GGTCTC 1 cut(s) 748
Eco47I GGWCC 1 cut(s) 118
Eco57I CTGAAG 2 cut(s) 265, 439
EcoRII CCWGG 1 cut(s) 757
EcoT14I CCWWGG 1 cut(s) 114
EcoT38I GRGCYC 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 114
FaeI CATG 3 cut(s) 713, 891, 946
FatI CATG 3 cut(s) 709, 887, 942
FauI CCCGC 1 cut(s) 607
FblI GTMKAC 1 cut(s) 432
Fnu4HI GCNGC 2 cut(s) 825, 898
FokI GGATG 6 cut(s) 84, 249, 260, 277, 641, 721
FriOI GRGCYC 1 cut(s) 164
Fsp4HI GCNGC 2 cut(s) 825, 898
FspI TGCGCA 1 cut(s) 930
GlaI GCGC 1 cut(s) 930
GluI GCNGC 2 cut(s) 825, 898
GsaI CCCAGC 1 cut(s) 440
GsuI CTGGAG 2 cut(s) 345, 570
HaeIII GGCC 2 cut(s) 29, 45
HapII CCGG 3 cut(s) 414, 603, 799
HhaI GCGC 1 cut(s) 931
Hin1II CATG 3 cut(s) 713, 891, 946
Hin6I GCGC 1 cut(s) 929
HinP1I GCGC 1 cut(s) 929
HindIII AAGCTT 1 cut(s) 957
HinfI GANTC 4 cut(s) 352, 378, 802, 833
HpaII CCGG 3 cut(s) 414, 603, 799
HphI GGTGA 4 cut(s) 103, 403, 428, 915
Hpy166II GTNNAC 2 cut(s) 71, 433
Hpy188I TCNGA 2 cut(s) 284, 458
Hpy188III TCNNGA 5 cut(s) 121, 324, 356, 747, 799
Hpy8I GTNNAC 2 cut(s) 71, 433
HpyAV CCTTC 1 cut(s) 459
HpyCH4III ACNGT 1 cut(s) 395
HpyCH4V TGCA 9 cut(s) 21, 35, 128, 197, 704, 734, 741, 900, 946
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 524
Hsp92II CATG 3 cut(s) 713, 891, 946
HspAI GCGC 1 cut(s) 929
Kpn2I TCCGGA 1 cut(s) 798
Kzo9I GATC 2 cut(s) 490, 874
LmnI GCTCC 2 cut(s) 140, 159
Lsp1109I GCAGC 2 cut(s) 811, 884
LweI GCATC 2 cut(s) 619, 743
MaeIII GTNAC 2 cut(s) 635, 666
MalI GATC 2 cut(s) 492, 876
MboI GATC 2 cut(s) 490, 874
MboII GAAGA 5 cut(s) 50, 440, 560, 664, 763
MfeI CAATTG 1 cut(s) 208
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 3 cut(s) 208, 675, 933
MlyI GAGTC 1 cut(s) 796
MnlI CCTC 7 cut(s) 220, 224, 227, 278, 452, 455, 545
MroI TCCGGA 1 cut(s) 798
MroXI GAANNNNTTC 1 cut(s) 278
MseI TTAA 4 cut(s) 53, 84, 204, 961
MslI CAYNNNNRTG 1 cut(s) 299
MspI CCGG 3 cut(s) 414, 603, 799
MspR9I CCNGG 1 cut(s) 759
MunI CAATTG 1 cut(s) 208
MvaI CCWGG 1 cut(s) 759
MwoI GCNNNNNNNGC 2 cut(s) 140, 524
NdeII GATC 2 cut(s) 490, 874
NlaIII CATG 3 cut(s) 713, 891, 946
NlaIV GGNNCC 2 cut(s) 161, 861
NmuCI GTSAC 1 cut(s) 666
NsbI TGCGCA 1 cut(s) 930
PaqCI CACCTGC 1 cut(s) 934
PdmI GAANNNNTTC 1 cut(s) 278
PfeI GAWTC 3 cut(s) 352, 378, 833
PflMI CCANNNNNTGG 1 cut(s) 103
PinAI ACCGGT 1 cut(s) 413
PkrI GCNGC 2 cut(s) 826, 899
PleI GAGTC 1 cut(s) 796
PpsI GAGTC 1 cut(s) 796
Psp6I CCWGG 1 cut(s) 757
PspFI CCCAGC 1 cut(s) 436
PspGI CCWGG 1 cut(s) 757
PspN4I GGNNCC 2 cut(s) 161, 861
PspPI GGNCC 1 cut(s) 118
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
RseI CAYNNNNRTG 1 cut(s) 299
SaqAI TTAA 4 cut(s) 53, 84, 204, 961
SatI GCNGC 2 cut(s) 825, 898
Sau3AI GATC 2 cut(s) 490, 874
Sau96I GGNCC 1 cut(s) 118
SchI GAGTC 1 cut(s) 796
ScrFI CCNGG 1 cut(s) 759
SduI GDGCHC 1 cut(s) 164
SfaNI GCATC 2 cut(s) 619, 743
SgrAI CRCCGGYG 1 cut(s) 413
SinI GGWCC 1 cut(s) 118
SmiMI CAYNNNNRTG 1 cut(s) 299
Sse9I AATT 3 cut(s) 208, 675, 933
SsiI CCGC 3 cut(s) 600, 626, 791
StyD4I CCNGG 1 cut(s) 757
StyI CCWWGG 1 cut(s) 114
TaaI ACNGT 1 cut(s) 395
TasI AATT 3 cut(s) 208, 675, 933
TfiI GAWTC 3 cut(s) 352, 378, 833
Tru1I TTAA 4 cut(s) 53, 84, 204, 961
Tru9I TTAA 4 cut(s) 53, 84, 204, 961
TscAI CASTG 1 cut(s) 53
TseFI GTSAC 1 cut(s) 666
TseI GCWGC 2 cut(s) 824, 897
Tsp45I GTSAC 1 cut(s) 666
TspDTI ATGAA 4 cut(s) 209, 279, 365, 631
TspGWI ACGGA 1 cut(s) 575
TspRI CASTG 1 cut(s) 53
Van91I CCANNNNNTGG 1 cut(s) 103
VpaK11BI GGWCC 1 cut(s) 118
XapI RAATTY 1 cut(s) 933
XmiI GTMKAC 1 cut(s) 432
XmnI GAANNNNTTC 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.