Rh7AG286000

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000996
Physical Location & Seq
Reverse (-)
30632175 .. 30636080
3906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG286000.1

Sequence Viewer

Length: 705 bp
ATGATAAATGCCAAAAATGCTCTACTCTCTGGTTGTTCGGCTGGAGGATTGGCTTCTATTCTTCAATGTGATAACTTCCGTTCTCTCTTACCCGCCGGAACTAATGTGAAATGCCTTGCGGATGCTGGTTACTTCATCAATGCGAAGACTATTACTGGGTCACAACAAATTGAAGCCTTTTATAGTCAAGTGGTTGCACTACATGGTTCAGCAAAGCATTTACCTGCATCCTGCACTTCAAGATTGAGACCAGGGTTGTGCTTCTTCCCACAAAATGTGATACCGCAACTCCGGACTCCTATTTTTTTGGTAAATGCTGCTTATGATTCGTGGCAGATAAAGAACATTTTGGCACCGAGTATTGCTGATCCTAAAGGCACATGGAAAAGCTGCAAGTCTGGTATAAAGAACTGCTCACCTGCGCAAATTCAAACCATGCAAGATTATAGGTTACAGTTCTTAGATGTACTCAATGGTGGTCTCACCAACTCTCCATCTCATGGAGCATTCATAGACTCTTGCTATGCTCACTGCCAAATCGGAACTCAAGAGACATGGATGGCATCTGACTCCCCGGTTGTCAGTAAGACGACAATTGCAAAGGCCGTCGGAGACTGGTATTACAACCGAAGTCCATCAAAAAAGATCGATTGTCCTTACCCTTGCAATCCTACCTGCAAAAATAAAGAATTTGATTCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

234

Amino Acids

25.4

Weight (kDa)

8.8

Isoelectric Point (pI)

53.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 1 - 227 1.1e-98 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 427
Acc16I TGCGCA 1 cut(s) 423
Acc36I ACCTGC 3 cut(s) 232, 427, 683
AccB1I GGYRCC 1 cut(s) 352
AccB7I CCANNNNNTGG 1 cut(s) 500
AccIII TCCGGA 1 cut(s) 291
AciI CCGC 3 cut(s) 93, 119, 284
AclWI GGATC 1 cut(s) 362
AcsI RAATTY 2 cut(s) 426, 689
AfaI GTAC 1 cut(s) 468
AfiI CCNNNNNNNGG 1 cut(s) 500
AgsI TTSAA 5 cut(s) 65, 173, 240, 431, 699
AjnI CCWGG 1 cut(s) 250
AluBI AGCT 1 cut(s) 390
AluI AGCT 1 cut(s) 390
Alw26I GTCTC 4 cut(s) 241, 485, 545, 606
AlwI GGATC 1 cut(s) 362
Aor13HI TCCGGA 1 cut(s) 291
AoxI GGCC 1 cut(s) 603
ApeKI GCWGC 2 cut(s) 317, 390
ApoI RAATTY 2 cut(s) 426, 689
AspLEI GCGC 1 cut(s) 424
AsuC2I CCSGG 1 cut(s) 575
AsuHPI GGTGA 2 cut(s) 408, 475
BanI GGYRCC 1 cut(s) 352
BbsI GAAGAC 1 cut(s) 152
BbvI GCAGC 2 cut(s) 304, 377
BccI CCATC 3 cut(s) 502, 553, 643
BceAI ACGGC 1 cut(s) 590
BciT130I CCWGG 1 cut(s) 252
BcnI CCSGG 1 cut(s) 575
BcoDI GTCTC 4 cut(s) 241, 485, 545, 606
BfaI CTAG 1 cut(s) 703
BfuAI ACCTGC 3 cut(s) 232, 427, 683
BisI GCNGC 2 cut(s) 318, 391
BlsI GCNGC 2 cut(s) 319, 392
Bme1390I CCNGG 2 cut(s) 252, 575
BmiI GGNNCC 1 cut(s) 354
BmrFI CCNGG 2 cut(s) 252, 575
BmrI ACTGGG 1 cut(s) 165
BmsI GCATC 3 cut(s) 112, 236, 572
BmuI ACTGGG 1 cut(s) 165
BpiI GAAGAC 1 cut(s) 152
BpmI CTGGAG 1 cut(s) 63
BpuEI CTTGAG 1 cut(s) 531
BpuMI CCSGG 1 cut(s) 575
Bsa29I ATCGAT 1 cut(s) 648
BsaI GGTCTC 2 cut(s) 241, 485
BsaJI CCNNGG 2 cut(s) 251, 573
BsaWI WCCGGW 1 cut(s) 291
BsaXI ACNNNNNCTCC 4 cut(s) 273, 303, 475, 505
Bsc4I CCNNNNNNNGG 1 cut(s) 500
Bse1I ACTGG 2 cut(s) 160, 620
BseAI TCCGGA 1 cut(s) 291
BseBI CCWGG 1 cut(s) 252
BseCI ATCGAT 1 cut(s) 648
BseDI CCNNGG 2 cut(s) 251, 573
BseGI GGATG 3 cut(s) 127, 227, 564
BseLI CCNNNNNNNGG 1 cut(s) 500
BseNI ACTGG 2 cut(s) 160, 620
BseXI GCAGC 2 cut(s) 304, 377
BsgI GTGCAG 1 cut(s) 217
BshFI GGCC 1 cut(s) 605
BshNI GGYRCC 1 cut(s) 352
BshVI ATCGAT 1 cut(s) 648
BsiSI CCGG 3 cut(s) 96, 292, 575
BslI CCNNNNNNNGG 1 cut(s) 500
BsmAI GTCTC 4 cut(s) 241, 485, 545, 606
BsmI GAATGC 1 cut(s) 506
BsnI GGCC 1 cut(s) 605
Bso31I GGTCTC 2 cut(s) 241, 485
Bsp13I TCCGGA 1 cut(s) 291
Bsp143I GATC 2 cut(s) 367, 645
BspACI CCGC 3 cut(s) 93, 119, 284
BspANI GGCC 1 cut(s) 605
BspDI ATCGAT 1 cut(s) 648
BspEI TCCGGA 1 cut(s) 291
BspLI GGNNCC 1 cut(s) 354
BspMI ACCTGC 3 cut(s) 232, 427, 683
BspPI GGATC 1 cut(s) 362
BspT107I GGYRCC 1 cut(s) 352
BspTNI GGTCTC 2 cut(s) 241, 485
BsrI ACTGG 2 cut(s) 160, 620
BssECI CCNNGG 2 cut(s) 251, 573
BssMI GATC 2 cut(s) 367, 645
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 1 cut(s) 456
BstDEI CTNAG 1 cut(s) 460
BstF5I GGATG 3 cut(s) 127, 227, 564
BstHHI GCGC 1 cut(s) 424
BstKTI GATC 2 cut(s) 370, 648
BstMAI GTCTC 4 cut(s) 241, 485, 545, 606
BstMBI GATC 2 cut(s) 367, 645
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNI CCWGG 1 cut(s) 252
BstSCI CCNGG 2 cut(s) 250, 573
BstV1I GCAGC 2 cut(s) 304, 377
BstV2I GAAGAC 1 cut(s) 152
Bsu15I ATCGAT 1 cut(s) 648
BsuRI GGCC 1 cut(s) 605
BsuTUI ATCGAT 1 cut(s) 648
BtsCI GGATG 3 cut(s) 127, 227, 564
BtsI GCAGTG 1 cut(s) 529
BtsIMutI CAGTG 1 cut(s) 529
BveI ACCTGC 3 cut(s) 232, 427, 683
CfoI GCGC 1 cut(s) 424
ClaI ATCGAT 1 cut(s) 648
Csp6I GTAC 1 cut(s) 467
CviAII CATG 5 cut(s) 203, 381, 436, 500, 555
CviJI RGCY 5 cut(s) 41, 53, 176, 390, 605
CviKI_1 RGCY 5 cut(s) 41, 53, 176, 390, 605
CviQI GTAC 1 cut(s) 467
DdeI CTNAG 1 cut(s) 460
DpnI GATC 2 cut(s) 369, 647
DpnII GATC 2 cut(s) 367, 645
Eco31I GGTCTC 2 cut(s) 241, 485
EcoRII CCWGG 1 cut(s) 250
FaeI CATG 5 cut(s) 206, 384, 439, 503, 558
FatI CATG 5 cut(s) 202, 380, 435, 499, 554
FauI CCCGC 1 cut(s) 100
Fnu4HI GCNGC 2 cut(s) 318, 391
FokI GGATG 3 cut(s) 134, 214, 571
Fsp4HI GCNGC 2 cut(s) 318, 391
FspBI CTAG 1 cut(s) 703
FspI TGCGCA 1 cut(s) 423
GlaI GCGC 1 cut(s) 423
GluI GCNGC 2 cut(s) 318, 391
GsuI CTGGAG 1 cut(s) 63
HaeIII GGCC 1 cut(s) 605
HapII CCGG 3 cut(s) 96, 292, 575
HhaI GCGC 1 cut(s) 424
Hin1II CATG 5 cut(s) 206, 384, 439, 503, 558
Hin6I GCGC 1 cut(s) 422
HinP1I GCGC 1 cut(s) 422
HinfI GANTC 5 cut(s) 295, 326, 515, 569, 695
HpaII CCGG 3 cut(s) 96, 292, 575
HphI GGTGA 2 cut(s) 408, 475
Hpy188I TCNGA 3 cut(s) 542, 568, 611
Hpy188III TCNNGA 3 cut(s) 240, 292, 548
Hpy99I CGWCG 1 cut(s) 611
HpyCH4III ACNGT 1 cut(s) 456
HpyCH4V TGCA 8 cut(s) 197, 227, 234, 393, 439, 599, 666, 678
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpyF3I CTNAG 1 cut(s) 460
Hsp92II CATG 5 cut(s) 206, 384, 439, 503, 558
HspAI GCGC 1 cut(s) 422
Kpn2I TCCGGA 1 cut(s) 291
Kzo9I GATC 2 cut(s) 367, 645
LmnI GCTCC 1 cut(s) 503
Lsp1109I GCAGC 2 cut(s) 304, 377
LweI GCATC 3 cut(s) 112, 236, 572
MaeI CTAG 1 cut(s) 703
MaeIII GTNAC 3 cut(s) 128, 159, 450
MalI GATC 2 cut(s) 369, 647
MboI GATC 2 cut(s) 367, 645
MboII GAAGA 3 cut(s) 53, 157, 256
MfeI CAATTG 1 cut(s) 594
MluCI AATT 4 cut(s) 168, 426, 594, 689
MlyI GAGTC 3 cut(s) 289, 509, 563
MmeI TCCRAC 1 cut(s) 589
MnlI CCTC 1 cut(s) 38
MroI TCCGGA 1 cut(s) 291
MspI CCGG 3 cut(s) 96, 292, 575
MspR9I CCNGG 2 cut(s) 252, 575
MunI CAATTG 1 cut(s) 594
Mva1269I GAATGC 1 cut(s) 506
MvaI CCWGG 1 cut(s) 252
MwoI GCNNNNNNNGC 1 cut(s) 17
NciI CCSGG 1 cut(s) 575
NdeII GATC 2 cut(s) 367, 645
NlaIII CATG 5 cut(s) 206, 384, 439, 503, 558
NlaIV GGNNCC 1 cut(s) 354
NmuCI GTSAC 1 cut(s) 159
NsbI TGCGCA 1 cut(s) 423
PaqCI CACCTGC 1 cut(s) 427
PctI GAATGC 1 cut(s) 506
PfeI GAWTC 2 cut(s) 326, 695
PflMI CCANNNNNTGG 1 cut(s) 500
PkrI GCNGC 2 cut(s) 319, 392
PleI GAGTC 3 cut(s) 289, 509, 563
PpsI GAGTC 3 cut(s) 289, 509, 563
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 1 cut(s) 354
RsaI GTAC 1 cut(s) 468
RsaNI GTAC 1 cut(s) 467
SatI GCNGC 2 cut(s) 318, 391
Sau3AI GATC 2 cut(s) 367, 645
SchI GAGTC 3 cut(s) 289, 509, 563
ScrFI CCNGG 2 cut(s) 252, 575
SetI ASST 5 cut(s) 226, 392, 421, 452, 677
SfaNI GCATC 3 cut(s) 112, 236, 572
SmlI CTYRAG 1 cut(s) 546
SmoI CTYRAG 1 cut(s) 546
Sse9I AATT 4 cut(s) 168, 426, 594, 689
SsiI CCGC 3 cut(s) 93, 119, 284
SspMI CTAG 1 cut(s) 703
StyD4I CCNGG 2 cut(s) 250, 573
TaaI ACNGT 1 cut(s) 456
TaqI TCGA 1 cut(s) 648
TasI AATT 4 cut(s) 168, 426, 594, 689
TatI WGTACW 1 cut(s) 466
TfiI GAWTC 2 cut(s) 326, 695
TscAI CASTG 1 cut(s) 536
TseFI GTSAC 1 cut(s) 159
TseI GCWGC 2 cut(s) 317, 390
Tsp45I GTSAC 1 cut(s) 159
TspDTI ATGAA 2 cut(s) 124, 499
TspGWI ACGGA 1 cut(s) 68
TspRI CASTG 1 cut(s) 536
Van91I CCANNNNNTGG 1 cut(s) 500
XapI RAATTY 2 cut(s) 426, 689
XspI CTAG 1 cut(s) 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.