Rorug07G0148900

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000996
Physical Location & Seq
Forward (+)
11796966 .. 11801042
4077 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0148900.1

Sequence Viewer

Length: 2415 bp
ATGGCTTCGAATGGTTCAGCAGGACATACATGTCCGCCTCCGATGAAGGCGACATCAAATGGTGTATTCCAGGGTGATGATCCCCTTCATTTTGCACTGCCTCTTGCGATTTTGCAGATATGTATAGTAGTTGCTGTCACTCGCGGTCTTGCCTATCTTCTGAGGCCACTAAGACAGCCGCGAGTCGTTGCTGAGATTATTGGAGGAGTACTACTTGGTCCATCAGCTCTAGGACGAAACAAAACTTATCTCCAGGCAATATTTCCACCAAAGAGTATCACGGTGTTAGACACCTTAGCAAACCTTGGTCTCCTGTTCTTTCTATTCTTGGCTGGCCTAGAAATAGATCCTAAAGCCATTCGCCAGACTGGAAAAAAAGCCCTTGCCATTGCCACAGTAGGAATCAGTCTCCCATTTGCATTAGGAATTGGTTCATCTTTTGTCCTCCGAGCAACCATATCCAAAGGGGTAGATTTTACTGCCTTTCTTGTGTTCATGGGTGTGGCCCTTTCTATAACTGCTTTCCCTGTTTTAGCACGTATTCTGGCCGAGCTGAAACTTTTGACCACTGAAATTGGAAGATTGGCTATGTCGGCTGCAGCAGTTAATGATGTGGCAGCCTGGGTTCTGCTTGCTCTTGCCATTGCCCTATCCGGCAATAACCAATCTCCCCTTGTTTCATTGTGGGTCTTATTGTCTGGATTTGCCTTTGTCATTTGTGCAATTCTAGTTGTACCCCCAATCTTCAAATGGATGGCTCAACGGTGTCATGAAGGTGAACCCATTGATGAGATCTACGTGTGCGCTACACTTACTGCTGTTCTGGCAGCTGGGTTTATAACTGATACAATTGGAATTCACGCCATGTTTGGTGCTTTTGTGATTGGAGTTCTTGTTCCGAAGGAGGGGCCATTTGCAGGTAGTCTTGTGGAAAAAGTAGAAGATCTTGTGTCTGGTCTCTTTCTTCCATTGTACTTTGTCTCAAGTGGACTGAAGACCAATGTAGCCACAATTCAGGGAGTTCAGTCTTGGGGTCTATTAGTTTTGGTCATATTTACTGCCTGTTTTGGGAAGATTTTCGGGACTGTTATGGTGTCTCTTTTATGCAAGGTGCCTGTCCGGGAGGCTTTAGCTCTTGGGTTCCTAATGAACACTAAAGGGTTGGTGGAGCTCATTGTCCTCAACATTGGCAAAGATAGAAAGGTTTTGAATGACCAGACTTTTGCCATTATGGTTCTAATGGCTCTGTTTACTACTTTCATCACCACACCTCTTGTCACTGCTGTGTATAAGCCAGCAAAAAGAGCCAGAATGGCTGATTACAAGTATAAAACAATTGAAAGGAAGAATACGAACTCCCAGTTAAGGATCTTGTCCTGTTTTCATAGTGCCAGAAACATTCCATCTATTATAAATCTGCTTGAGGCCTCACGAGGCACAAAGAAGCGGGAAGGCCTTTGTGTTTATGCAATGCACCTGAAGGAGCTCTCTGAGAGGTCATCGGCTATACTAATGGTACACAAGGCAAGAAGGAATGGGTTACCCTTCTGGAATAAGGGACAGCGATCAAATTCTGACAATGTTGTTGTGGCATTCGAGGCATACCAACAATTAAGCCGGGTGTCCATCAGGCCAATGACTGAAATCTCTTCAGTTTCTAACATGCACGAGGACATTTGTGCCACTGCTGAGAGCAAAAGAGCTGCAATCATAATTCTTCCATTCCATAAGCACCAGAGGCTAGATGGTACATTAGAGACTACCCGAAATGACTTCCGCGGGGTTAACAAGAGAGTTCTTGAGCATGCACCATGCTCAGTTGGAATTCTAGTCGACCGCGGGCTTGGTGGAGCTACGCATATTGCTGCAAGTAATGTTTCTTATTTCATTACAGTTCTTTACTTTGGGGGCCGTGATGACCGTGAAGCCCTTGCCTATGGGGCTCGCATGGCCGAGCACCCTGGTATCCGTTTAGTGGTCATTCGCTTCTTAGTGGAACCTGAGATTGTTGGGGAGATATCCACAATTAATATTGATCACAATTCTGGCAGAAAAGTGGGGTCAGTAGATGAGGAGTTCCTCGCTGAATTCAAGCATACAATTGCCAATGATAACTCAATCACATACGAAGAGAAGGTAGTCAGAAATGAGGCACAAACCGTTGGTGTGATTCGTGAGATAGGCCGATGCAATCTGTTTCTGGTCGGGCGAAGTCCTGGTGGTGAAGTAGCCTTGGCCCTAAATAGGAGGAGTGAGTGCCCGGAATTGGGGCCTGTGGGAAGCTTGTTGATTTCGCCAGATTTCCCTACACAAGCATCAGTGTTGGTAGTGCAACAGTACAACGGTCAGGTACCGTTGAATTTGGCCTCGGAGATGGAGGAAGTGTCACCTGATGACGATACAGAATCGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

804

Amino Acids

87.09

Weight (kDa)

8.74

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 47 - 426 1.9e-68 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 484 - 625 2.1e-47 Plant cation/H(+) antiporter domain
CHX17_C PF23259 629 - 782 4.3e-31 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 839, 1412
AasI GACNNNNNNGTC 1 cut(s) 30
Acc36I ACCTGC 1 cut(s) 908
Acc65I GGTACC 1 cut(s) 2350
AccB1I GGYRCC 2 cut(s) 1111, 2350
AccI GTMKAC 1 cut(s) 1833
AccII CGCG 4 cut(s) 144, 181, 1779, 1839
AciI CCGC 8 cut(s) 35, 144, 179, 1447, 1777, 1779, 1837, 1839
AclWI GGATC 3 cut(s) 74, 341, 1376
AcoI YGGCCR 2 cut(s) 546, 1950
AcsI RAATTY 5 cut(s) 855, 1570, 1824, 2087, 2359
AcuI CTGAAG 3 cut(s) 1013, 1499, 1635
AfaI GTAC 7 cut(s) 210, 735, 974, 1518, 1750, 2339, 2352
AfiI CCNNNNNNNGG 8 cut(s) 905, 917, 1068, 1365, 1975, 2244, 2266, 2267
AflIII ACRYGT 2 cut(s) 29, 798
AgsI TTSAA 5 cut(s) 748, 1210, 1340, 2092, 2359
AjnI CCWGG 5 cut(s) 69, 252, 620, 1960, 2215
AleI CACNNNNGTG 1 cut(s) 1283
AloI GAACNNNNNNTCC 2 cut(s) 879, 911
AluBI AGCT 9 cut(s) 227, 553, 830, 1133, 1171, 1486, 1703, 1853, 2283
AluI AGCT 9 cut(s) 227, 553, 830, 1133, 1171, 1486, 1703, 1853, 2283
Alw21I GWGCWC 3 cut(s) 1173, 1488, 1959
Alw26I GTCTC 6 cut(s) 314, 413, 962, 985, 1101, 1751
AlwI GGATC 3 cut(s) 74, 341, 1376
ApeKI GCWGC 6 cut(s) 596, 599, 617, 827, 1703, 1865
ApoI RAATTY 5 cut(s) 855, 1570, 1824, 2087, 2359
ArsI GACNNNNNNTTYG 2 cut(s) 1205, 1237
AseI ATTAAT 1 cut(s) 2028
Asp700I GAANNNNTTC 2 cut(s) 430, 1076
Asp718I GGTACC 1 cut(s) 2350
AspLEI GCGC 1 cut(s) 806
AspS9I GGNCC 6 cut(s) 218, 505, 908, 1909, 2236, 2270
AsuC2I CCSGG 3 cut(s) 1121, 1619, 2261
AsuHPI GGTGA 5 cut(s) 86, 788, 1255, 2234, 2379
AsuII TTCGAA 1 cut(s) 8
AvaII GGWCC 1 cut(s) 218
BaeGI GKGCMC 1 cut(s) 2261
BaeI ACNNNNGTAYC 2 cut(s) 1948, 1981
BanI GGYRCC 2 cut(s) 1111, 2350
BanII GRGCYC 3 cut(s) 1173, 1488, 1945
BauI CACGAG 2 cut(s) 1431, 1667
BbsI GAAGAC 1 cut(s) 1001
Bbv12I GWGCWC 3 cut(s) 1173, 1488, 1959
BbvI GCAGC 6 cut(s) 583, 611, 629, 839, 1690, 1852
BccI CCATC 6 cut(s) 229, 748, 1411, 1634, 1739, 2368
BceAI ACGGC 1 cut(s) 1896
BciT130I CCWGG 5 cut(s) 71, 254, 622, 1962, 2217
BciVI GTATCC 1 cut(s) 1976
BclI TGATCA 1 cut(s) 2035
BcnI CCSGG 3 cut(s) 1121, 1619, 2261
BcoDI GTCTC 6 cut(s) 314, 413, 962, 985, 1101, 1751
BfaI CTAG 6 cut(s) 230, 338, 728, 1742, 1829, 2413
BfmI CTRYAG 1 cut(s) 597
BfuAI ACCTGC 1 cut(s) 908
BfuI GTATCC 1 cut(s) 1976
BglI GCCNNNNNGGC 2 cut(s) 1313, 1940
BglII AGATCT 2 cut(s) 792, 943
BisI GCNGC 7 cut(s) 179, 597, 600, 618, 828, 1704, 1866
BlsI GCNGC 7 cut(s) 180, 598, 601, 619, 829, 1705, 1867
BmcAI AGTACT 1 cut(s) 210
Bme1390I CCNGG 8 cut(s) 71, 254, 622, 1121, 1619, 1962, 2217, 2261
Bme18I GGWCC 1 cut(s) 218
BmgT120I GGNCC 6 cut(s) 218, 505, 908, 1909, 2236, 2270
BmiI GGNNCC 7 cut(s) 909, 1113, 1142, 1910, 1998, 2271, 2352
BmrFI CCNGG 8 cut(s) 71, 254, 622, 1121, 1619, 1962, 2217, 2261
BmrI ACTGGG 1 cut(s) 1354
BmsI GCATC 2 cut(s) 2177, 2324
BmuI ACTGGG 1 cut(s) 1354
BpiI GAAGAC 1 cut(s) 1001
BpmI CTGGAG 1 cut(s) 236
Bpu10I CCTNAGC 1 cut(s) 295
Bpu14I TTCGAA 1 cut(s) 8
BpuEI CTTGAG 3 cut(s) 967, 1442, 1820
BpuMI CCSGG 3 cut(s) 1121, 1619, 2261
BsaAI YACGTR 2 cut(s) 539, 799
BsaI GGTCTC 2 cut(s) 314, 962
BsaJI CCNNGG 8 cut(s) 70, 304, 621, 1777, 1837, 1960, 2232, 2367
BsaXI ACNNNNNCTCC 4 cut(s) 879, 909, 2369, 2399
Bsc4I CCNNNNNNNGG 8 cut(s) 905, 917, 1068, 1365, 1975, 2244, 2266, 2267
Bse1I ACTGG 2 cut(s) 373, 1360
Bse3DI GCAATG 3 cut(s) 387, 642, 1476
BseBI CCWGG 5 cut(s) 71, 254, 622, 1962, 2217
BseDI CCNNGG 8 cut(s) 70, 304, 621, 1777, 1837, 1960, 2232, 2367
BseGI GGATG 1 cut(s) 759
BseLI CCNNNNNNNGG 8 cut(s) 905, 917, 1068, 1365, 1975, 2244, 2266, 2267
BseMI GCAATG 3 cut(s) 387, 642, 1476
BseMII CTCAG 6 cut(s) 152, 183, 1482, 1680, 1830, 1992
BseNI ACTGG 2 cut(s) 373, 1360
BseRI GAGGAG 3 cut(s) 219, 2087, 2263
BseSI GKGCMC 1 cut(s) 2261
BseXI GCAGC 6 cut(s) 583, 611, 629, 839, 1690, 1852
BseYI CCCAGC 1 cut(s) 830
Bsh1236I CGCG 4 cut(s) 144, 181, 1779, 1839
Bsh1285I CGRYCG 1 cut(s) 1837
BshNI GGYRCC 2 cut(s) 1111, 2350
BsiEI CGRYCG 1 cut(s) 1837
BsiHKAI GWGCWC 3 cut(s) 1173, 1488, 1959
BsiSI CCGG 4 cut(s) 654, 1120, 1618, 2261
BslFI GGGAC 2 cut(s) 1096, 1572
BslI CCNNNNNNNGG 8 cut(s) 905, 917, 1068, 1365, 1975, 2244, 2266, 2267
BsmAI GTCTC 6 cut(s) 314, 413, 962, 985, 1101, 1751
BsmFI GGGAC 2 cut(s) 1096, 1572
BsmI GAATGC 1 cut(s) 1592
Bso31I GGTCTC 2 cut(s) 314, 962
Bsp119I TTCGAA 1 cut(s) 8
Bsp1286I GDGCHC 5 cut(s) 1173, 1488, 1945, 1959, 2261
Bsp143I GATC 7 cut(s) 79, 346, 792, 943, 1368, 1565, 2035
BspACI CCGC 8 cut(s) 35, 144, 179, 1447, 1777, 1779, 1837, 1839
BspCNI CTCAG 6 cut(s) 153, 184, 1483, 1681, 1829, 1993
BspFNI CGCG 4 cut(s) 144, 181, 1779, 1839
BspHI TCATGA 1 cut(s) 769
BspLI GGNNCC 7 cut(s) 909, 1113, 1142, 1910, 1998, 2271, 2352
BspMAI CTGCAG 1 cut(s) 601
BspMI ACCTGC 1 cut(s) 908
BspPI GGATC 3 cut(s) 74, 341, 1376
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 2 cut(s) 1111, 2350
BspTNI GGTCTC 2 cut(s) 314, 962
BsrDI GCAATG 3 cut(s) 387, 642, 1476
BsrI ACTGG 2 cut(s) 373, 1360
BssECI CCNNGG 8 cut(s) 70, 304, 621, 1777, 1837, 1960, 2232, 2367
BssMI GATC 7 cut(s) 79, 346, 792, 943, 1368, 1565, 2035
BssSI CACGAG 2 cut(s) 1431, 1667
BssT1I CCWWGG 2 cut(s) 304, 2232
Bst2BI CACGAG 2 cut(s) 1431, 1667
Bst2UI CCWGG 5 cut(s) 71, 254, 622, 1962, 2217
Bst6I CTCTTC 2 cut(s) 1654, 2124
BstBAI YACGTR 2 cut(s) 539, 799
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 6 cut(s) 334, 633, 1296, 1806, 1841, 1945
BstDEI CTNAG 9 cut(s) 161, 170, 192, 295, 1491, 1689, 1816, 1990, 2001
BstDSI CCRYGG 2 cut(s) 1777, 1837
BstEII GGTNACC 1 cut(s) 1539
BstF5I GGATG 1 cut(s) 759
BstFNI CGCG 4 cut(s) 144, 181, 1779, 1839
BstHHI GCGC 1 cut(s) 806
BstKTI GATC 7 cut(s) 82, 349, 795, 946, 1371, 1568, 2038
BstMAI GTCTC 6 cut(s) 314, 413, 962, 985, 1101, 1751
BstMBI GATC 7 cut(s) 79, 346, 792, 943, 1368, 1565, 2035
BstMCI CGRYCG 1 cut(s) 1837
BstMWI GCNNNNNNNGC 8 cut(s) 593, 824, 1304, 1313, 1598, 1738, 1940, 1949
BstNI CCWGG 5 cut(s) 71, 254, 622, 1962, 2217
BstNSI RCATGY 3 cut(s) 33, 1666, 1808
BstPI GGTNACC 1 cut(s) 1539
BstSCI CCNGG 8 cut(s) 69, 252, 620, 1119, 1617, 1960, 2215, 2259
BstSFI CTRYAG 1 cut(s) 597
BstSLI GKGCMC 1 cut(s) 2261
BstUI CGCG 4 cut(s) 144, 181, 1779, 1839
BstV1I GCAGC 6 cut(s) 583, 611, 629, 839, 1690, 1852
BstV2I GAAGAC 1 cut(s) 1001
BstX2I RGATCY 4 cut(s) 346, 792, 943, 1368
BstYI RGATCY 4 cut(s) 346, 792, 943, 1368
BsuI GTATCC 1 cut(s) 1976
BtgI CCRYGG 2 cut(s) 1777, 1837
BtsCI GGATG 1 cut(s) 759
BtsI GCAGTG 3 cut(s) 95, 1278, 1683
BtsIMutI CAGTG 5 cut(s) 95, 567, 1278, 1683, 2325
BveI ACCTGC 1 cut(s) 908
Cac8I GCNNGC 6 cut(s) 334, 633, 1296, 1806, 1841, 1945
CciI TCATGA 1 cut(s) 769
CfoI GCGC 1 cut(s) 806
Cfr13I GGNCC 6 cut(s) 218, 505, 908, 1909, 2236, 2270
Cfr42I CCGCGG 2 cut(s) 1780, 1840
Csp6I GTAC 7 cut(s) 209, 734, 973, 1517, 1749, 2338, 2351
CspCI CAANNNNNGTGG 4 cut(s) 556, 591, 1255, 1290
CviAII CATG 8 cut(s) 30, 496, 770, 865, 1663, 1805, 1812, 1948
CviQI GTAC 7 cut(s) 209, 734, 973, 1517, 1749, 2338, 2351
DdeI CTNAG 9 cut(s) 161, 170, 192, 295, 1491, 1689, 1816, 1990, 2001
DpnI GATC 7 cut(s) 81, 348, 794, 945, 1370, 1567, 2037
DpnII GATC 7 cut(s) 79, 346, 792, 943, 1368, 1565, 2035
DrdI GACNNNNNNGTC 1 cut(s) 30
DseDI GACNNNNNNGTC 1 cut(s) 30
EaeI YGGCCR 2 cut(s) 546, 1950
Eam1104I CTCTTC 2 cut(s) 1654, 2124
EarI CTCTTC 2 cut(s) 1654, 2124
EciI GGCGGA 1 cut(s) 24
Ecl136II GAGCTC 2 cut(s) 1171, 1486
Eco130I CCWWGG 2 cut(s) 304, 2232
Eco147I AGGCCT 2 cut(s) 1427, 1455
Eco24I GRGCYC 3 cut(s) 1173, 1488, 1945
Eco31I GGTCTC 2 cut(s) 314, 962
Eco32I GATATC 1 cut(s) 2019
Eco47I GGWCC 1 cut(s) 218
Eco53kI GAGCTC 2 cut(s) 1171, 1486
Eco57I CTGAAG 3 cut(s) 1013, 1499, 1635
Eco91I GGTNACC 1 cut(s) 1539
EcoICRI GAGCTC 2 cut(s) 1171, 1486
EcoO109I RGGNCCY 1 cut(s) 2270
EcoO65I GGTNACC 1 cut(s) 1539
EcoRI GAATTC 3 cut(s) 855, 1824, 2087
EcoRII CCWGG 5 cut(s) 69, 252, 620, 1960, 2215
EcoRV GATATC 1 cut(s) 2019
EcoT14I CCWWGG 2 cut(s) 304, 2232
EcoT38I GRGCYC 3 cut(s) 1173, 1488, 1945
ErhI CCWWGG 2 cut(s) 304, 2232
FaeI CATG 8 cut(s) 33, 499, 773, 868, 1666, 1808, 1815, 1951
FaqI GGGAC 2 cut(s) 1096, 1572
FatI CATG 8 cut(s) 29, 495, 769, 864, 1662, 1804, 1811, 1947
FauI CCCGC 3 cut(s) 1440, 1772, 1832
FbaI TGATCA 1 cut(s) 2035
FblI GTMKAC 1 cut(s) 1833
Fnu4HI GCNGC 7 cut(s) 179, 597, 600, 618, 828, 1704, 1866
FokI GGATG 1 cut(s) 766
FriOI GRGCYC 3 cut(s) 1173, 1488, 1945
Fsp4HI GCNGC 7 cut(s) 179, 597, 600, 618, 828, 1704, 1866
FspBI CTAG 6 cut(s) 230, 338, 728, 1742, 1829, 2413
GlaI GCGC 1 cut(s) 805
GluI GCNGC 7 cut(s) 179, 597, 600, 618, 828, 1704, 1866
GsaI CCCAGC 1 cut(s) 834
GsuI CTGGAG 1 cut(s) 236
HapII CCGG 4 cut(s) 654, 1120, 1618, 2261
HhaI GCGC 1 cut(s) 806
Hin1II CATG 8 cut(s) 33, 499, 773, 868, 1666, 1808, 1815, 1951
Hin6I GCGC 1 cut(s) 804
HinP1I GCGC 1 cut(s) 804
HincII GTYRAC 2 cut(s) 1786, 1834
HindII GTYRAC 2 cut(s) 1786, 1834
HindIII AAGCTT 1 cut(s) 2281
HinfI GANTC 4 cut(s) 183, 402, 2170, 2405
HpaI GTTAAC 1 cut(s) 1786
HpaII CCGG 4 cut(s) 654, 1120, 1618, 2261
HphI GGTGA 5 cut(s) 86, 788, 1255, 2234, 2379
Hpy166II GTNNAC 6 cut(s) 779, 989, 1251, 1519, 1786, 1834
Hpy188I TCNGA 8 cut(s) 42, 162, 449, 900, 1492, 1576, 2144, 2371
Hpy188III TCNNGA 7 cut(s) 699, 770, 1081, 1431, 1549, 1799, 2174
Hpy8I GTNNAC 6 cut(s) 779, 989, 1251, 1519, 1786, 1834
HpyAV CCTTC 9 cut(s) 40, 95, 767, 895, 1445, 1474, 1524, 1555, 2128
HpyCH4IV ACGT 2 cut(s) 538, 798
HpyF10VI GCNNNNNNNGC 8 cut(s) 593, 824, 1304, 1313, 1598, 1738, 1940, 1949
HpyF3I CTNAG 9 cut(s) 161, 170, 192, 295, 1491, 1689, 1816, 1990, 2001
HpySE526I ACGT 2 cut(s) 538, 798
Hsp92II CATG 8 cut(s) 33, 499, 773, 868, 1666, 1808, 1815, 1951
HspAI GCGC 1 cut(s) 804
KpnI GGTACC 1 cut(s) 2354
Ksp22I TGATCA 1 cut(s) 2035
KspAI GTTAAC 1 cut(s) 1786
KspI CCGCGG 2 cut(s) 1780, 1840
Kzo9I GATC 7 cut(s) 79, 346, 792, 943, 1368, 1565, 2035
LmnI GCTCC 3 cut(s) 1168, 1483, 1850
Lsp1109I GCAGC 6 cut(s) 583, 611, 629, 839, 1690, 1852
LweI GCATC 2 cut(s) 2177, 2324
MaeI CTAG 6 cut(s) 230, 338, 728, 1742, 1829, 2413
MaeII ACGT 2 cut(s) 538, 798
MaeIII GTNAC 4 cut(s) 136, 1276, 1539, 2385
MalI GATC 7 cut(s) 81, 348, 794, 945, 1370, 1567, 2037
MboI GATC 7 cut(s) 79, 346, 792, 943, 1368, 1565, 2035
MfeI CAATTG 3 cut(s) 849, 1335, 2100
MflI RGATCY 4 cut(s) 346, 792, 943, 1368
MhlI GDGCHC 5 cut(s) 1173, 1488, 1945, 1959, 2261
MlyI GAGTC 1 cut(s) 192
MmeI TCCRAC 1 cut(s) 1801
MroXI GAANNNNTTC 2 cut(s) 430, 1076
MseI TTAA 5 cut(s) 606, 1364, 1613, 1785, 2028
MslI CAYNNNNRTG 3 cut(s) 500, 774, 1283
MspA1I CMGCKG 3 cut(s) 830, 1779, 1839
MspI CCGG 4 cut(s) 654, 1120, 1618, 2261
MspR9I CCNGG 8 cut(s) 71, 254, 622, 1121, 1619, 1962, 2217, 2261
MunI CAATTG 3 cut(s) 849, 1335, 2100
Mva1269I GAATGC 1 cut(s) 1592
MvaI CCWGG 5 cut(s) 71, 254, 622, 1962, 2217
MvnI CGCG 4 cut(s) 144, 181, 1779, 1839
MwoI GCNNNNNNNGC 8 cut(s) 593, 824, 1304, 1313, 1598, 1738, 1940, 1949
NciI CCSGG 3 cut(s) 1121, 1619, 2261
NdeII GATC 7 cut(s) 79, 346, 792, 943, 1368, 1565, 2035
NlaIII CATG 8 cut(s) 33, 499, 773, 868, 1666, 1808, 1815, 1951
NlaIV GGNNCC 7 cut(s) 909, 1113, 1142, 1910, 1998, 2271, 2352
NmeAIII GCCGAG 2 cut(s) 574, 1978
NmuCI GTSAC 3 cut(s) 136, 1276, 2385
NspI RCATGY 3 cut(s) 33, 1666, 1808
NspV TTCGAA 1 cut(s) 8
OliI CACNNNNGTG 1 cut(s) 1283
PaeI GCATGC 1 cut(s) 1808
PagI TCATGA 1 cut(s) 769
PceI AGGCCT 2 cut(s) 1427, 1455
PciI ACATGT 1 cut(s) 29
PctI GAATGC 1 cut(s) 1592
PdmI GAANNNNTTC 2 cut(s) 430, 1076
PfeI GAWTC 3 cut(s) 402, 2170, 2405
PfoI TCCNGGA 1 cut(s) 1119
PkrI GCNGC 7 cut(s) 180, 598, 601, 619, 829, 1705, 1867
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
Ppu21I YACGTR 2 cut(s) 539, 799
PscI ACATGT 1 cut(s) 29
PshBI ATTAAT 1 cut(s) 2028
PsiI TTATAA 2 cut(s) 839, 1412
Psp124BI GAGCTC 2 cut(s) 1173, 1488
Psp6I CCWGG 5 cut(s) 69, 252, 620, 1960, 2215
PspEI GGTNACC 1 cut(s) 1539
PspFI CCCAGC 1 cut(s) 830
PspGI CCWGG 5 cut(s) 69, 252, 620, 1960, 2215
PspN4I GGNNCC 7 cut(s) 909, 1113, 1142, 1910, 1998, 2271, 2352
PspPI GGNCC 6 cut(s) 218, 505, 908, 1909, 2236, 2270
PstI CTGCAG 1 cut(s) 601
PsuI RGATCY 4 cut(s) 346, 792, 943, 1368
PvuII CAGCTG 1 cut(s) 830
RsaI GTAC 7 cut(s) 210, 735, 974, 1518, 1750, 2339, 2352
RsaNI GTAC 7 cut(s) 209, 734, 973, 1517, 1749, 2338, 2351
RseI CAYNNNNRTG 3 cut(s) 500, 774, 1283
SacI GAGCTC 2 cut(s) 1173, 1488
SacII CCGCGG 2 cut(s) 1780, 1840
SalI GTCGAC 1 cut(s) 1832
SaqAI TTAA 5 cut(s) 606, 1364, 1613, 1785, 2028
SatI GCNGC 7 cut(s) 179, 597, 600, 618, 828, 1704, 1866
Sau3AI GATC 7 cut(s) 79, 346, 792, 943, 1368, 1565, 2035
Sau96I GGNCC 6 cut(s) 218, 505, 908, 1909, 2236, 2270
ScaI AGTACT 1 cut(s) 210
SchI GAGTC 1 cut(s) 192
ScrFI CCNGG 8 cut(s) 71, 254, 622, 1121, 1619, 1962, 2217, 2261
SduI GDGCHC 5 cut(s) 1173, 1488, 1945, 1959, 2261
SfaNI GCATC 2 cut(s) 2177, 2324
SfcI CTRYAG 1 cut(s) 597
Sfr303I CCGCGG 2 cut(s) 1780, 1840
SfuI TTCGAA 1 cut(s) 8
SgrBI CCGCGG 2 cut(s) 1780, 1840
SinI GGWCC 1 cut(s) 218
SmiMI CAYNNNNRTG 3 cut(s) 500, 774, 1283
SmlI CTYRAG 3 cut(s) 982, 1421, 1799
SmoI CTYRAG 3 cut(s) 982, 1421, 1799
SphI GCATGC 1 cut(s) 1808
SseBI AGGCCT 2 cut(s) 1427, 1455
SsiI CCGC 8 cut(s) 35, 144, 179, 1447, 1777, 1779, 1837, 1839
SspI AATATT 2 cut(s) 261, 2032
SspMI CTAG 6 cut(s) 230, 338, 728, 1742, 1829, 2413
SstI GAGCTC 2 cut(s) 1173, 1488
StuI AGGCCT 2 cut(s) 1427, 1455
StyD4I CCNGG 8 cut(s) 69, 252, 620, 1119, 1617, 1960, 2215, 2259
StyI CCWWGG 2 cut(s) 304, 2232
TaiI ACGT 2 cut(s) 541, 801
TaqI TCGA 4 cut(s) 8, 1596, 1833, 2408
TatI WGTACW 3 cut(s) 208, 972, 2337
TauI GCSGC 1 cut(s) 181
TfiI GAWTC 3 cut(s) 402, 2170, 2405
Tru1I TTAA 5 cut(s) 606, 1364, 1613, 1785, 2028
Tru9I TTAA 5 cut(s) 606, 1364, 1613, 1785, 2028
TscAI CASTG 5 cut(s) 102, 574, 1285, 1690, 2325
TseFI GTSAC 3 cut(s) 136, 1276, 2385
TseI GCWGC 6 cut(s) 596, 599, 617, 827, 1703, 1865
Tsp45I GTSAC 3 cut(s) 136, 1276, 2385
TspGWI ACGGA 1 cut(s) 1958
TspRI CASTG 5 cut(s) 102, 574, 1285, 1690, 2325
VpaK11BI GGWCC 1 cut(s) 218
VspI ATTAAT 1 cut(s) 2028
XapI RAATTY 5 cut(s) 855, 1570, 1824, 2087, 2359
XceI RCATGY 3 cut(s) 33, 1666, 1808
XcmI CCANNNNNNNNNTGG 2 cut(s) 747, 1742
XmiI GTMKAC 1 cut(s) 1833
XmnI GAANNNNTTC 2 cut(s) 430, 1076
XspI CTAG 6 cut(s) 230, 338, 728, 1742, 1829, 2413
ZrmI AGTACT 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.