MD08G1205500.v1.1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000996
Physical Location & Seq
Reverse (-)
26600551 .. 26605105
4555 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1205500.v1.1.491

Sequence Viewer

Length: 1443 bp
ATGTTTTTCGTTAAAATTCTCCTACAAATACTCTCGAAATATCTCTTCACTGGAAAGCACTTAGCTTTCTCTTCACATCACACTTTAATTCCTTGCTTTTGCTCGGAAAAACATATACTCTCGCACAAGATATATATCCTATACACATATATATATAATTGGAATAAGAAGTTCAAGCATTCAAGGTTTCTCTCACGTTCTTTCTTCTACCATTTCCTTCAGATTATTCTCTATTACCAGTTTATAATGGCCTGTTCAAGATTAGGCCAATGGTTAACTCTTCTAGTATGTTTACTGGTGTTGCTGAAAGCAGAAAGCCATCCTGTGGCACTCACTCTCGTCGAGAATGCAGTGGCTAAAGGAGCTGTATGTTTGGATGGAAGCGCATCCGCTTACCACTTCGACAAGGGATTTGGTGCCGGAATCAACAATTGGTTGGTTCATGTTGAGGGTGGAGGATGGTGTAACACCGTTGAAGATTGCCTTGATCGCAAGAGTAATTACCGCGGTTCATCAACCAAAATGGAAAAGACAATGTCTTTTTCTGGGGTTCTTGGTAACAAGCCAAAGACTAATCCAGACTTCTACAATTGGAATAGAATCAAAGTTAGGTACTGCGATGGCTCGTCATTCACCGGTGATGTTGAAGCAGTCGACCCAGCAACAAATCTTCACTTCAGAGGAGCAAGGGTTTTCCGTGCCATCATTGATGATTTATTGGCAAAGGGAATGAAAAATGCTCAAAACGCTCTTCTATCTGGTTGTTCGGCTGGTGGACTAACCTCGATTCTTCATTGTGATAACTTTCGTTCTCTCCTACCTGTGGGTACTAAAGTAAAATGTCTAGCAGATGCTGGTTACTTCATTAATGCGAAGAGTTTGTCTGGATCACAACACATTCAAGGCTTTTACAGTGAAGTGGTTGCGCTACATGGTTCGGCGAAGAACTTACCTGCATCCTGCACTTCAAGAATGAGTCCAGGGCTGTGCTTCTTCCCACAAAATGTGGTACCTCAAACCCAGACGCCAATATTTTTGGTCAATGCAGCTTATGATTCGTGGCAGATAAAGAACATTTTGGCACCGGGTGTTGCTGATCCTAAGGGTGCCTGGAAAAAGTGCAAGCTTAATATAAAGAACTGCTCACCCAGTCAACTTCAAACCTTGCAAGAATATAGGTTACAGTTCTTAAAAACAATTGCCAGTTCGAGCACTTCTTCATCTCATGGAGCGTTCATAGACTCTTGCTTTGCCCACTGCCAAATTGGAACGCAGGAGACATGGCTGGCAGCTGACTCTCCTATGTTGAGCAAGACGACAATTGCAAACGCAGTTGGAGACTGGTATTACGACCGCACTGCGTTCAAAAAGTTTGATTGCCCTTACCCGTGCAATCCCACTTGCAAAAACCGTGAATTTGACTCGAACGATCAGGAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

481

Amino Acids

53.58

Weight (kDa)

8.96

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 103 - 469 1.7e-175 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 245
Acc36I ACCTGC 1 cut(s) 961
Acc65I GGTACC 1 cut(s) 1009
AccB1I GGYRCC 4 cut(s) 416, 1009, 1081, 1106
AccB7I CCANNNNNTGG 1 cut(s) 325
AccI GTMKAC 1 cut(s) 654
AccII CGCG 1 cut(s) 507
AciI CCGC 4 cut(s) 390, 505, 507, 1354
AclWI GGATC 2 cut(s) 895, 1091
AcsI RAATTY 2 cut(s) 15, 1415
AcuI CTGAAG 2 cut(s) 203, 661
AcyI GRCGYC 1 cut(s) 1025
AdeI CACNNNGTG 1 cut(s) 1088
AfaI GTAC 3 cut(s) 614, 829, 1011
AfiI CCNNNNNNNGG 2 cut(s) 325, 823
AgeI ACCGGT 1 cut(s) 635
AgsI TTSAA 9 cut(s) 175, 183, 258, 476, 647, 902, 969, 1160, 1366
AjnI CCWGG 2 cut(s) 979, 1109
AjuI GAANNNNNNNTTGG 2 cut(s) 415, 447
AluBI AGCT 5 cut(s) 65, 365, 1049, 1126, 1292
AluI AGCT 5 cut(s) 65, 365, 1049, 1126, 1292
Alw21I GWGCWC 1 cut(s) 1214
Alw26I GTCTC 2 cut(s) 1271, 1332
AlwI GGATC 2 cut(s) 895, 1091
AlwNI CAGNNNCTG 1 cut(s) 854
AoxI GGCC 2 cut(s) 249, 265
ApeKI GCWGC 2 cut(s) 1046, 1289
ApoI RAATTY 2 cut(s) 15, 1415
ArsI GACNNNNNNTTYG 6 cut(s) 395, 427, 866, 898, 1232, 1264
AseI ATTAAT 1 cut(s) 867
AsiGI ACCGGT 1 cut(s) 635
Asp718I GGTACC 1 cut(s) 1009
AspLEI GCGC 2 cut(s) 386, 928
AsuC2I CCSGG 1 cut(s) 1086
AsuHPI GGTGA 3 cut(s) 625, 650, 1137
AxyI CCTNAGG 1 cut(s) 1101
BaeI ACNNNNGTAYC 2 cut(s) 819, 852
BanI GGYRCC 4 cut(s) 416, 1009, 1081, 1106
Bbv12I GWGCWC 1 cut(s) 1214
BbvI GCAGC 2 cut(s) 1058, 1301
BccI CCATC 5 cut(s) 327, 371, 453, 614, 710
BcgI CGANNNNNNTGC 2 cut(s) 1340, 1374
BciT130I CCWGG 2 cut(s) 981, 1111
BcnI CCSGG 1 cut(s) 1086
BcoDI GTCTC 2 cut(s) 1271, 1332
BfaI CTAG 2 cut(s) 284, 845
BfuAI ACCTGC 1 cut(s) 961
BisI GCNGC 2 cut(s) 1047, 1290
BlsI GCNGC 2 cut(s) 1048, 1291
Bme1390I CCNGG 3 cut(s) 981, 1086, 1111
BmiI GGNNCC 4 cut(s) 418, 1011, 1083, 1108
BmrFI CCNGG 3 cut(s) 981, 1086, 1111
BmrI ACTGGG 1 cut(s) 1143
BmsI GCATC 3 cut(s) 395, 841, 965
BmuI ACTGGG 1 cut(s) 1143
BpuMI CCSGG 1 cut(s) 1086
BsaBI GATNNNNATC 1 cut(s) 134
BsaHI GRCGYC 1 cut(s) 1025
BsaJI CCNNGG 2 cut(s) 505, 980
BsaWI WCCGGW 1 cut(s) 635
BsaXI ACNNNNNCTCC 2 cut(s) 675, 705
Bsc4I CCNNNNNNNGG 2 cut(s) 325, 823
Bse118I RCCGGY 1 cut(s) 635
Bse1I ACTGG 6 cut(s) 55, 238, 300, 1149, 1203, 1346
Bse21I CCTNAGG 1 cut(s) 1101
Bse8I GATNNNNATC 1 cut(s) 134
BseBI CCWGG 2 cut(s) 981, 1111
BseDI CCNNGG 2 cut(s) 505, 980
BseGI GGATG 5 cut(s) 319, 382, 386, 464, 956
BseJI GATNNNNATC 1 cut(s) 134
BseLI CCNNNNNNNGG 2 cut(s) 325, 823
BseNI ACTGG 6 cut(s) 55, 238, 300, 1149, 1203, 1346
BseRI GAGGAG 1 cut(s) 696
BseXI GCAGC 2 cut(s) 1058, 1301
BseYI CCCAGC 1 cut(s) 658
BsgI GTGCAG 1 cut(s) 946
Bsh1236I CGCG 1 cut(s) 507
Bsh1285I CGRYCG 1 cut(s) 1354
BshFI GGCC 2 cut(s) 251, 267
BshNI GGYRCC 4 cut(s) 416, 1009, 1081, 1106
BshTI ACCGGT 1 cut(s) 635
BsiEI CGRYCG 1 cut(s) 1354
BsiHKAI GWGCWC 1 cut(s) 1214
BsiSI CCGG 3 cut(s) 420, 636, 1085
BslI CCNNNNNNNGG 2 cut(s) 325, 823
BsmAI GTCTC 2 cut(s) 1271, 1332
BsmI GAATGC 2 cut(s) 178, 352
BsnI GGCC 2 cut(s) 251, 267
Bsp1286I GDGCHC 1 cut(s) 1214
Bsp143I GATC 4 cut(s) 487, 887, 1096, 1429
BspACI CCGC 4 cut(s) 390, 505, 507, 1354
BspANI GGCC 2 cut(s) 251, 267
BspFNI CGCG 1 cut(s) 507
BspLI GGNNCC 4 cut(s) 418, 1011, 1083, 1108
BspMI ACCTGC 1 cut(s) 961
BspPI GGATC 2 cut(s) 895, 1091
BspQI GCTCTTC 1 cut(s) 756
BspT107I GGYRCC 4 cut(s) 416, 1009, 1081, 1106
BsrFI RCCGGY 1 cut(s) 635
BsrI ACTGG 6 cut(s) 55, 238, 300, 1149, 1203, 1346
BssAI RCCGGY 1 cut(s) 635
BssECI CCNNGG 2 cut(s) 505, 980
BssMI GATC 4 cut(s) 487, 887, 1096, 1429
BssNI GRCGYC 1 cut(s) 1025
Bst2UI CCWGG 2 cut(s) 981, 1111
Bst4CI ACNGT 4 cut(s) 472, 914, 1185, 1412
Bst6I CTCTTC 5 cut(s) 50, 76, 285, 756, 869
BstACI GRCGYC 1 cut(s) 1025
BstC8I GCNNGC 2 cut(s) 1124, 1287
BstDEI CTNAG 2 cut(s) 61, 1101
BstDSI CCRYGG 1 cut(s) 505
BstF5I GGATG 5 cut(s) 319, 382, 386, 464, 956
BstFNI CGCG 1 cut(s) 507
BstHHI GCGC 2 cut(s) 386, 928
BstKTI GATC 4 cut(s) 490, 890, 1099, 1432
BstMAI GTCTC 2 cut(s) 1271, 1332
BstMBI GATC 4 cut(s) 487, 887, 1096, 1429
BstMCI CGRYCG 1 cut(s) 1354
BstMWI GCNNNNNNNGC 3 cut(s) 362, 489, 746
BstNI CCWGG 2 cut(s) 981, 1111
BstSCI CCNGG 3 cut(s) 979, 1084, 1109
BstUI CGCG 1 cut(s) 507
BstV1I GCAGC 2 cut(s) 1058, 1301
Bsu36I CCTNAGG 1 cut(s) 1101
BsuRI GGCC 2 cut(s) 251, 267
BtgI CCRYGG 1 cut(s) 505
BtgZI GCGATG 1 cut(s) 633
BtsCI GGATG 5 cut(s) 319, 382, 386, 464, 956
BtsI GCAGTG 3 cut(s) 357, 1255, 1356
BtsIMutI CAGTG 5 cut(s) 48, 357, 919, 1255, 1356
BveI ACCTGC 1 cut(s) 961
Cac8I GCNNGC 2 cut(s) 1124, 1287
CaiI CAGNNNCTG 1 cut(s) 854
CfoI GCGC 2 cut(s) 386, 928
Cfr10I RCCGGY 1 cut(s) 635
Cfr42I CCGCGG 1 cut(s) 508
CseI GACGC 1 cut(s) 1033
Csp6I GTAC 3 cut(s) 613, 828, 1010
CspAI ACCGGT 1 cut(s) 635
CviAII CATG 4 cut(s) 443, 932, 1226, 1281
CviQI GTAC 3 cut(s) 613, 828, 1010
DdeI CTNAG 2 cut(s) 61, 1101
DpnI GATC 4 cut(s) 489, 889, 1098, 1431
DpnII GATC 4 cut(s) 487, 887, 1096, 1429
DraIII CACNNNGTG 1 cut(s) 1088
Eam1104I CTCTTC 5 cut(s) 50, 76, 285, 756, 869
EarI CTCTTC 5 cut(s) 50, 76, 285, 756, 869
Eco57I CTGAAG 2 cut(s) 203, 661
Eco81I CCTNAGG 1 cut(s) 1101
EcoRII CCWGG 2 cut(s) 979, 1109
FaeI CATG 4 cut(s) 446, 935, 1229, 1284
FalI AAGNNNNNCTT 2 cut(s) 468, 500
FatI CATG 4 cut(s) 442, 931, 1225, 1280
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 2 cut(s) 1047, 1290
FokI GGATG 5 cut(s) 306, 373, 389, 471, 943
Fsp4HI GCNGC 2 cut(s) 1047, 1290
FspBI CTAG 2 cut(s) 284, 845
GlaI GCGC 2 cut(s) 385, 927
GluI GCNGC 2 cut(s) 1047, 1290
GsaI CCCAGC 1 cut(s) 662
HaeIII GGCC 2 cut(s) 251, 267
HapII CCGG 3 cut(s) 420, 636, 1085
HgaI GACGC 1 cut(s) 1033
HhaI GCGC 2 cut(s) 386, 928
Hin1I GRCGYC 1 cut(s) 1025
Hin1II CATG 4 cut(s) 446, 935, 1229, 1284
Hin6I GCGC 2 cut(s) 384, 926
HinP1I GCGC 2 cut(s) 384, 926
HincII GTYRAC 3 cut(s) 276, 655, 1154
HindII GTYRAC 3 cut(s) 276, 655, 1154
HindIII AAGCTT 1 cut(s) 1124
HinfI GANTC 8 cut(s) 423, 600, 787, 976, 1055, 1241, 1295, 1421
HpaI GTTAAC 1 cut(s) 276
HpaII CCGG 3 cut(s) 420, 636, 1085
HphI GGTGA 3 cut(s) 625, 650, 1137
Hpy166II GTNNAC 5 cut(s) 276, 293, 655, 776, 1154
Hpy188I TCNGA 3 cut(s) 106, 222, 680
Hpy188III TCNNGA 7 cut(s) 34, 258, 343, 578, 885, 969, 1433
Hpy8I GTNNAC 5 cut(s) 276, 293, 655, 776, 1154
Hpy99I CGWCG 1 cut(s) 344
HpyAV CCTTC 1 cut(s) 227
HpyCH4III ACNGT 4 cut(s) 472, 914, 1185, 1412
HpyCH4IV ACGT 1 cut(s) 196
HpyCH4V TGCA 9 cut(s) 350, 956, 963, 1046, 1122, 1168, 1325, 1392, 1404
HpyF10VI GCNNNNNNNGC 3 cut(s) 362, 489, 746
HpyF3I CTNAG 2 cut(s) 61, 1101
HpySE526I ACGT 1 cut(s) 196
Hsp92I GRCGYC 1 cut(s) 1025
Hsp92II CATG 4 cut(s) 446, 935, 1229, 1284
HspAI GCGC 2 cut(s) 384, 926
KpnI GGTACC 1 cut(s) 1013
KspAI GTTAAC 1 cut(s) 276
KspI CCGCGG 1 cut(s) 508
Kzo9I GATC 4 cut(s) 487, 887, 1096, 1429
LguI GCTCTTC 1 cut(s) 756
LmnI GCTCC 3 cut(s) 362, 683, 1229
Lsp1109I GCAGC 2 cut(s) 1058, 1301
LweI GCATC 3 cut(s) 395, 841, 965
MaeI CTAG 2 cut(s) 284, 845
MaeII ACGT 1 cut(s) 196
MaeIII GTNAC 4 cut(s) 464, 557, 857, 1179
MalI GATC 4 cut(s) 489, 889, 1098, 1431
MboI GATC 4 cut(s) 487, 887, 1096, 1429
MfeI CAATTG 4 cut(s) 430, 589, 1197, 1320
MhlI GDGCHC 1 cut(s) 1214
MlyI GAGTC 4 cut(s) 985, 1235, 1289, 1415
MmeI TCCRAC 1 cut(s) 1315
MnlI CCTC 5 cut(s) 442, 449, 674, 793, 1023
MseI TTAA 7 cut(s) 12, 86, 275, 867, 1128, 1190, 1441
MspA1I CMGCKG 2 cut(s) 507, 1292
MspI CCGG 3 cut(s) 420, 636, 1085
MspR9I CCNGG 3 cut(s) 981, 1086, 1111
MunI CAATTG 4 cut(s) 430, 589, 1197, 1320
Mva1269I GAATGC 2 cut(s) 178, 352
MvaI CCWGG 2 cut(s) 981, 1111
MvnI CGCG 1 cut(s) 507
MwoI GCNNNNNNNGC 3 cut(s) 362, 489, 746
NciI CCSGG 1 cut(s) 1086
NdeII GATC 4 cut(s) 487, 887, 1096, 1429
NlaIII CATG 4 cut(s) 446, 935, 1229, 1284
NlaIV GGNNCC 4 cut(s) 418, 1011, 1083, 1108
PciSI GCTCTTC 1 cut(s) 756
PctI GAATGC 2 cut(s) 178, 352
PfeI GAWTC 4 cut(s) 423, 600, 787, 1055
PflFI GACNNNGTC 1 cut(s) 535
PflMI CCANNNNNTGG 1 cut(s) 325
PinAI ACCGGT 1 cut(s) 635
PkrI GCNGC 2 cut(s) 1048, 1291
PleI GAGTC 4 cut(s) 984, 1235, 1289, 1415
PpsI GAGTC 4 cut(s) 984, 1235, 1289, 1415
PshBI ATTAAT 1 cut(s) 867
PsiI TTATAA 1 cut(s) 245
Psp6I CCWGG 2 cut(s) 979, 1109
PspFI CCCAGC 1 cut(s) 658
PspGI CCWGG 2 cut(s) 979, 1109
PspN4I GGNNCC 4 cut(s) 418, 1011, 1083, 1108
PstNI CAGNNNCTG 1 cut(s) 854
PsyI GACNNNGTC 1 cut(s) 535
PvuII CAGCTG 1 cut(s) 1292
RsaI GTAC 3 cut(s) 614, 829, 1011
RsaNI GTAC 3 cut(s) 613, 828, 1010
SacII CCGCGG 1 cut(s) 508
SalI GTCGAC 1 cut(s) 653
SapI GCTCTTC 1 cut(s) 756
SaqAI TTAA 7 cut(s) 12, 86, 275, 867, 1128, 1190, 1441
SatI GCNGC 2 cut(s) 1047, 1290
Sau3AI GATC 4 cut(s) 487, 887, 1096, 1429
SchI GAGTC 4 cut(s) 985, 1235, 1289, 1415
ScrFI CCNGG 3 cut(s) 981, 1086, 1111
SduI GDGCHC 1 cut(s) 1214
SfaNI GCATC 3 cut(s) 395, 841, 965
Sfr303I CCGCGG 1 cut(s) 508
SgrAI CRCCGGYG 1 cut(s) 635
SgrBI CCGCGG 1 cut(s) 508
SsiI CCGC 4 cut(s) 390, 505, 507, 1354
SspI AATATT 1 cut(s) 1032
SspMI CTAG 2 cut(s) 284, 845
StyD4I CCNGG 3 cut(s) 979, 1084, 1109
TaaI ACNGT 4 cut(s) 472, 914, 1185, 1412
TaiI ACGT 1 cut(s) 199
TaqI TCGA 7 cut(s) 35, 342, 402, 654, 785, 1208, 1424
TfiI GAWTC 4 cut(s) 423, 600, 787, 1055
Tru1I TTAA 7 cut(s) 12, 86, 275, 867, 1128, 1190, 1441
Tru9I TTAA 7 cut(s) 12, 86, 275, 867, 1128, 1190, 1441
TscAI CASTG 5 cut(s) 55, 357, 919, 1262, 1363
TseI GCWGC 2 cut(s) 1046, 1289
TspDTI ATGAA 7 cut(s) 431, 501, 746, 782, 853, 1209, 1225
TspGWI ACGGA 1 cut(s) 686
TspRI CASTG 5 cut(s) 55, 357, 919, 1262, 1363
Tth111I GACNNNGTC 1 cut(s) 535
Van91I CCANNNNNTGG 1 cut(s) 325
VspI ATTAAT 1 cut(s) 867
XapI RAATTY 2 cut(s) 15, 1415
XcmI CCANNNNNNNNNTGG 1 cut(s) 1262
XmiI GTMKAC 1 cut(s) 654
XspI CTAG 2 cut(s) 284, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.