Rroxscaffold_3G00244820

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000996
Physical Location & Seq
Forward (+)
37639283 .. 37643431
4149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00244820.1

Sequence Viewer

Length: 1377 bp
ATGCTTTCCCTTCTCTTCTGTAATCCCCATTGGCCCATTACTCCCCCATTAACTCCCCGTTCATCACTCATCAGTTATCAAGTACACACAGTACAAATAACAACCCCATGTTCTGAACTGGGTTTCTCCCACATTCTTCATCACTCTGTCTCTGCCATTTTCTCTCCTTCATTCAGGCAGATGGGTGATGCAAGATTAGCGCAATGGTTGAATCTTCTAGTATGTTCAGTGATAATGCTGAAAGCAACGGGAGTTCCTGTCGGAATCACTATCCTTAAGACTGCGGTTGCCAAAGGAGCCGTTTGTTTGGATGGGAGCCCCCCAGCTTACCATTGGGATAAGGGGTTTGGTTCAGGAGTTAACAATTGGTTGGTTCACATTGAGGGAGGAGGATGGTGTAACAATGTCACAAATTGCCTCGGCCGTAAGAACACTCGTTTAGGTTCATCAAAGAAAATGCTGAAGGAAATTCCTTTTTCTGGAATTCTGAACAACAGGCAATCACTTAATCCAGGCTTTTACAATTGGAATAGAATCAAAGTCAGGTACTGCGATGGGTCATCATTCACTGGAGATGTTGAAGCAGTACACCAAGCTACTGGTCTTTACTTCAGAGGAGCAAGGGTTTTTCGAGCTGTTATTGCTGATTTATTAGCAAAAGGAATGGGGAAAGCTAAAAATGCTGTCCTCTCTGGTTGTTCTGCTGGTGGATTGACCTCTATTCTTCATTGTGATAACTTCCGTGCTCTCTTACCTTCTGGAACCGTTGTGAAATGTGTTTCAGATGCTGGTTACTTTATTAATGTAAAGGACATTTCTGGAGCACAACACATTCAACAGTATTTTGCTGATGTTGTTACAACACATGGTTCTGCGAAGAACTTGCCTCCATCTTGCACTTCAAAATTGAGACCAGATTTGTGTTTCTTCCCTCAATATGTGGTACCAAAGATTCGGACACCAATATTCTATGTAAATGCAGCCTATGATTCATGGCAGATAAGGAACAGTTTGGTACCAAGTGTTGCGGATCCTCATGGAACTTGGCGAAACTGCAAGCTCAATATAAAGAACTGCTCACCCGCTCAGCTCAAAACCATTCAAAACTTCAGGTTGCAATTTTTAACTGCAATTGGTGGTGTGAGCAACTCTGCAGTAAAGGGAATGTTCATAGACTCTTGCTATCTACACTGTCAAACTGAATTGCAGGAGACTTGGTTAATGCCTAATTCTCCACTTCTTGACAAAATGACAATTGCAGGGGCAGTTAGAGACTGGTTTTATGACCGAACTCCATTCCAAAAGATAGACTGTCCTTACTCTTGCAATCCCACTTGCCATGCTCCGATGTTTGATCCAAATGTGCACTTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

458

Amino Acids

50.48

Weight (kDa)

9.12

Isoelectric Point (pI)

40.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 82 - 447 1.5e-172 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 943, 1015
AccB1I GGYRCC 2 cut(s) 943, 1015
AccBSI CCGCTC 1 cut(s) 1085
AciI CCGC 3 cut(s) 284, 1028, 1083
AclWI GGATC 3 cut(s) 1025, 1038, 1349
AcoI YGGCCR 1 cut(s) 421
AcsI RAATTY 2 cut(s) 468, 483
AcuI CTGAAG 3 cut(s) 482, 595, 1093
AfaI GTAC 6 cut(s) 84, 93, 548, 588, 945, 1017
AfiI CCNNNNNNNGG 1 cut(s) 479
AflII CTTAAG 1 cut(s) 275
AgsI TTSAA 5 cut(s) 211, 581, 836, 903, 1103
AjnI CCWGG 1 cut(s) 511
AluBI AGCT 6 cut(s) 326, 596, 635, 674, 1060, 1090
AluI AGCT 6 cut(s) 326, 596, 635, 674, 1060, 1090
Alw21I GWGCWC 3 cut(s) 748, 826, 1368
Alw26I GTCTC 4 cut(s) 154, 904, 1205, 1266
Alw44I GTGCAC 1 cut(s) 1364
AlwI GGATC 3 cut(s) 1025, 1038, 1349
AlwNI CAGNNNCTG 2 cut(s) 549, 788
AoxI GGCC 2 cut(s) 32, 421
ApaLI GTGCAC 1 cut(s) 1364
ApeKI GCWGC 1 cut(s) 980
ApoI RAATTY 2 cut(s) 468, 483
AseI ATTAAT 1 cut(s) 801
Asp718I GGTACC 2 cut(s) 943, 1015
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 1 cut(s) 33
AsuHPI GGTGA 2 cut(s) 197, 1071
BaeGI GKGCMC 1 cut(s) 1368
BamHI GGATCC 1 cut(s) 1030
BanI GGYRCC 2 cut(s) 943, 1015
BanII GRGCYC 1 cut(s) 320
Bbv12I GWGCWC 3 cut(s) 748, 826, 1368
BbvI GCAGC 1 cut(s) 992
BccI CCATC 5 cut(s) 175, 305, 387, 548, 898
BceAI ACGGC 2 cut(s) 284, 408
BcgI CGANNNNNNTGC 2 cut(s) 865, 899
BciT130I CCWGG 1 cut(s) 513
BcoDI GTCTC 4 cut(s) 154, 904, 1205, 1266
BfaI CTAG 1 cut(s) 218
BfmI CTRYAG 1 cut(s) 1152
BfrI CTTAAG 1 cut(s) 275
BisI GCNGC 1 cut(s) 981
BlpI GCTNAGC 1 cut(s) 1086
BlsI GCNGC 1 cut(s) 982
Bme1390I CCNGG 1 cut(s) 513
BmgT120I GGNCC 1 cut(s) 33
BmiI GGNNCC 6 cut(s) 298, 317, 763, 945, 1017, 1032
BmrFI CCNGG 1 cut(s) 513
BmrI ACTGGG 1 cut(s) 128
BmsI GCATC 2 cut(s) 178, 775
BmuI ACTGGG 1 cut(s) 128
BpmI CTGGAG 2 cut(s) 591, 840
Bpu1102I GCTNAGC 1 cut(s) 1086
BsaI GGTCTC 1 cut(s) 904
BsaJI CCNNGG 1 cut(s) 418
BsaXI ACNNNNNCTCC 6 cut(s) 243, 273, 609, 639, 1202, 1232
Bsc4I CCNNNNNNNGG 1 cut(s) 479
Bse1I ACTGG 4 cut(s) 123, 574, 604, 1280
Bse3DI GCAATG 1 cut(s) 209
BseBI CCWGG 1 cut(s) 513
BseDI CCNNGG 1 cut(s) 418
BseGI GGATG 2 cut(s) 316, 398
BseLI CCNNNNNNNGG 1 cut(s) 479
BseMI GCAATG 1 cut(s) 209
BseMII CTCAG 1 cut(s) 1100
BseNI ACTGG 4 cut(s) 123, 574, 604, 1280
BseRI GAGGAG 2 cut(s) 402, 630
BseSI GKGCMC 1 cut(s) 1368
BseX3I CGGCCG 1 cut(s) 421
BseXI GCAGC 1 cut(s) 992
BseYI CCCAGC 1 cut(s) 322
Bsh1285I CGRYCG 1 cut(s) 424
BshFI GGCC 2 cut(s) 34, 423
BshNI GGYRCC 2 cut(s) 943, 1015
BsiEI CGRYCG 1 cut(s) 424
BsiHKAI GWGCWC 3 cut(s) 748, 826, 1368
BslI CCNNNNNNNGG 1 cut(s) 479
BsmAI GTCTC 4 cut(s) 154, 904, 1205, 1266
BsnI GGCC 2 cut(s) 34, 423
Bso31I GGTCTC 1 cut(s) 904
Bsp1286I GDGCHC 4 cut(s) 320, 748, 826, 1368
Bsp143I GATC 2 cut(s) 1030, 1354
Bsp1720I GCTNAGC 1 cut(s) 1086
BspACI CCGC 3 cut(s) 284, 1028, 1083
BspANI GGCC 2 cut(s) 34, 423
BspCNI CTCAG 1 cut(s) 1099
BspLI GGNNCC 6 cut(s) 298, 317, 763, 945, 1017, 1032
BspMAI CTGCAG 1 cut(s) 1156
BspPI GGATC 3 cut(s) 1025, 1038, 1349
BspT107I GGYRCC 2 cut(s) 943, 1015
BspTI CTTAAG 1 cut(s) 275
BspTNI GGTCTC 1 cut(s) 904
BsrBI CCGCTC 1 cut(s) 1085
BsrDI GCAATG 1 cut(s) 209
BsrI ACTGG 4 cut(s) 123, 574, 604, 1280
BssECI CCNNGG 1 cut(s) 418
BssMI GATC 2 cut(s) 1030, 1354
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 6 cut(s) 91, 766, 840, 1010, 1193, 1313
Bst6I CTCTTC 1 cut(s) 20
BstAFI CTTAAG 1 cut(s) 275
BstC8I GCNNGC 1 cut(s) 1058
BstDEI CTNAG 1 cut(s) 1086
BstF5I GGATG 2 cut(s) 316, 398
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 2 cut(s) 1033, 1357
BstMAI GTCTC 4 cut(s) 154, 904, 1205, 1266
BstMBI GATC 2 cut(s) 1030, 1354
BstMCI CGRYCG 1 cut(s) 424
BstMWI GCNNNNNNNGC 4 cut(s) 197, 296, 641, 680
BstNI CCWGG 1 cut(s) 513
BstSCI CCNGG 1 cut(s) 511
BstSFI CTRYAG 1 cut(s) 1152
BstSLI GKGCMC 1 cut(s) 1368
BstV1I GCAGC 1 cut(s) 992
BstX2I RGATCY 1 cut(s) 1030
BstXI CCANNNNNNTGG 1 cut(s) 599
BstYI RGATCY 1 cut(s) 1030
BstZI CGGCCG 1 cut(s) 421
BsuRI GGCC 2 cut(s) 34, 423
BtgZI GCGATG 1 cut(s) 567
BtsCI GGATG 2 cut(s) 316, 398
BtsIMutI CAGTG 3 cut(s) 234, 567, 1189
Cac8I GCNNGC 1 cut(s) 1058
CaiI CAGNNNCTG 2 cut(s) 549, 788
CfoI GCGC 1 cut(s) 202
Cfr13I GGNCC 1 cut(s) 33
Csp6I GTAC 6 cut(s) 83, 92, 547, 587, 944, 1016
CviAII CATG 5 cut(s) 108, 866, 993, 1037, 1340
CviQI GTAC 6 cut(s) 83, 92, 547, 587, 944, 1016
DdeI CTNAG 1 cut(s) 1086
DpnI GATC 2 cut(s) 1032, 1356
DpnII GATC 2 cut(s) 1030, 1354
EaeI YGGCCR 1 cut(s) 421
EagI CGGCCG 1 cut(s) 421
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 421
Eco24I GRGCYC 1 cut(s) 320
Eco31I GGTCTC 1 cut(s) 904
Eco52I CGGCCG 1 cut(s) 421
Eco57I CTGAAG 3 cut(s) 482, 595, 1093
EcoRI GAATTC 1 cut(s) 483
EcoRII CCWGG 1 cut(s) 511
EcoT38I GRGCYC 1 cut(s) 320
FaeI CATG 5 cut(s) 111, 869, 996, 1040, 1343
FatI CATG 5 cut(s) 107, 865, 992, 1036, 1339
FauI CCCGC 1 cut(s) 1090
Fnu4HI GCNGC 1 cut(s) 981
FokI GGATG 2 cut(s) 323, 405
FriOI GRGCYC 1 cut(s) 320
Fsp4HI GCNGC 1 cut(s) 981
FspBI CTAG 1 cut(s) 218
GlaI GCGC 1 cut(s) 201
GluI GCNGC 1 cut(s) 981
GsaI CCCAGC 1 cut(s) 326
GsuI CTGGAG 2 cut(s) 591, 840
HaeIII GGCC 2 cut(s) 34, 423
HhaI GCGC 1 cut(s) 202
Hin1II CATG 5 cut(s) 111, 869, 996, 1040, 1343
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 6 cut(s) 211, 264, 534, 952, 989, 1175
HpaI GTTAAC 1 cut(s) 361
HphI GGTGA 2 cut(s) 197, 1071
Hpy166II GTNNAC 5 cut(s) 85, 361, 376, 589, 1366
Hpy188I TCNGA 7 cut(s) 115, 263, 489, 614, 784, 957, 1347
Hpy188III TCNNGA 5 cut(s) 354, 480, 759, 819, 1241
Hpy8I GTNNAC 5 cut(s) 85, 361, 376, 589, 1366
HpyAV CCTTC 4 cut(s) 20, 177, 457, 765
HpyCH4III ACNGT 6 cut(s) 91, 766, 840, 1010, 1193, 1313
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 296, 641, 680
HpyF3I CTNAG 1 cut(s) 1086
Hsp92II CATG 5 cut(s) 111, 869, 996, 1040, 1343
HspAI GCGC 1 cut(s) 200
KpnI GGTACC 2 cut(s) 947, 1019
KspAI GTTAAC 1 cut(s) 361
Kzo9I GATC 2 cut(s) 1030, 1354
LmnI GCTCC 5 cut(s) 296, 315, 617, 821, 1348
Lsp1109I GCAGC 1 cut(s) 992
LweI GCATC 2 cut(s) 178, 775
MaeI CTAG 1 cut(s) 218
MaeIII GTNAC 4 cut(s) 398, 406, 791, 856
MalI GATC 2 cut(s) 1032, 1356
MbiI CCGCTC 1 cut(s) 1085
MboI GATC 2 cut(s) 1030, 1354
MboII GAAGA 6 cut(s) 7, 128, 206, 716, 889, 919
MfeI CAATTG 4 cut(s) 364, 523, 1131, 1254
MflI RGATCY 1 cut(s) 1030
MhlI GDGCHC 4 cut(s) 320, 748, 826, 1368
MlyI GAGTC 1 cut(s) 1169
MmeI TCCRAC 1 cut(s) 241
MseI TTAA 7 cut(s) 50, 276, 360, 507, 801, 1124, 1220
MspCI CTTAAG 1 cut(s) 275
MspR9I CCNGG 1 cut(s) 513
MunI CAATTG 4 cut(s) 364, 523, 1131, 1254
MvaI CCWGG 1 cut(s) 513
MwoI GCNNNNNNNGC 4 cut(s) 197, 296, 641, 680
NdeII GATC 2 cut(s) 1030, 1354
NlaIII CATG 5 cut(s) 111, 869, 996, 1040, 1343
NlaIV GGNNCC 6 cut(s) 298, 317, 763, 945, 1017, 1032
NmeAIII GCCGAG 1 cut(s) 399
NmuCI GTSAC 1 cut(s) 406
PfeI GAWTC 5 cut(s) 211, 264, 534, 952, 989
PkrI GCNGC 1 cut(s) 982
PleI GAGTC 1 cut(s) 1169
PpsI GAGTC 1 cut(s) 1169
PshBI ATTAAT 1 cut(s) 801
Psp6I CCWGG 1 cut(s) 511
PspFI CCCAGC 1 cut(s) 322
PspGI CCWGG 1 cut(s) 511
PspN4I GGNNCC 6 cut(s) 298, 317, 763, 945, 1017, 1032
PspPI GGNCC 1 cut(s) 33
PstI CTGCAG 1 cut(s) 1156
PstNI CAGNNNCTG 2 cut(s) 549, 788
PsuI RGATCY 1 cut(s) 1030
RsaI GTAC 6 cut(s) 84, 93, 548, 588, 945, 1017
RsaNI GTAC 6 cut(s) 83, 92, 547, 587, 944, 1016
SaqAI TTAA 7 cut(s) 50, 276, 360, 507, 801, 1124, 1220
SatI GCNGC 1 cut(s) 981
Sau3AI GATC 2 cut(s) 1030, 1354
Sau96I GGNCC 1 cut(s) 33
SchI GAGTC 1 cut(s) 1169
ScrFI CCNGG 1 cut(s) 513
SduI GDGCHC 4 cut(s) 320, 748, 826, 1368
SfaNI GCATC 2 cut(s) 178, 775
SfcI CTRYAG 1 cut(s) 1152
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
SsiI CCGC 3 cut(s) 284, 1028, 1083
SspI AATATT 1 cut(s) 966
SspMI CTAG 1 cut(s) 218
StyD4I CCNGG 1 cut(s) 511
TaaI ACNGT 6 cut(s) 91, 766, 840, 1010, 1193, 1313
TaqI TCGA 1 cut(s) 631
TaqII GACCGA 1 cut(s) 1302
TatI WGTACW 3 cut(s) 82, 91, 586
TfiI GAWTC 5 cut(s) 211, 264, 534, 952, 989
Tru1I TTAA 7 cut(s) 50, 276, 360, 507, 801, 1124, 1220
Tru9I TTAA 7 cut(s) 50, 276, 360, 507, 801, 1124, 1220
TscAI CASTG 3 cut(s) 234, 574, 1196
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 1 cut(s) 980
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 7 cut(s) 51, 128, 159, 435, 716, 981, 1159
TspGWI ACGGA 1 cut(s) 731
TspRI CASTG 3 cut(s) 234, 574, 1196
Vha464I CTTAAG 1 cut(s) 275
VneI GTGCAC 1 cut(s) 1364
VspI ATTAAT 1 cut(s) 801
XapI RAATTY 2 cut(s) 468, 483
XcmI CCANNNNNNNNNTGG 1 cut(s) 330
XspI CTAG 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.