MD06G1012900.v1.1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000996
Physical Location & Seq
Forward (+)
1634018 .. 1639343
5326 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1012900.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGCTGCTGAAAGCAGCCGCATTACCTGTGGGAATAACTTACGTTCATAGTGCGGTTGCCAAAGGAGCCGTTTGCTTGGATGGGAGTCCCCCAGCTTACCACCTTGATAAGGGATATGGTTCAGGAATTAACAATTGGTTGGTTCACATTGAGGGAGGAGGATGGTGTAACAATATCACAACTTGTCTTGTCCGTAAAAACAACCGTTTAGGTTCATCTAAGAAAATGCTCAAGGAAATTGCATTTTCTGGGATTCTGAACAGCAGGCATATATTTAATCCGGACTTTTACAACTGGAATAGAGTCAAAGTTAGGTATTGCGACGGGGCATCATTCACTGGTGATGTTGCAGCTGTAAACCTAGCTACCGGTCTTTTCTTCAGAGGAGCAAGGATTTTTCGTGCAGTCATTGAAGAACTATTAGCAAAAGGAATGGGGAAGGCTAAAAACGCTATTCTCTCTGGTTGTTCTGCTGGTGGATTGGCGTCCATTCTTCACTGTGACAACTTCCATGCCCTCTTACCTGTTGGAGCTAAAGTGAAATGCGTTTCAGACGCTGGTTACTTCATTAATGCGAAGGATGTTTCTGGAGCACGACATGTTGAACAGTTTTTTAGTCAAGTGGTTGCAACACATGGTTCGGCGAAGAATTTGCCTCCATCTTGCACTTCAAAATTGAGTCCAGGTTTGTGCTTTTTCCCGCAATATTTTGCCTCACAAATCCGGACGCCAATGTTCTATGTAAATGCAGCCTACGATTCATGGCAGATAAAGAACATCTTGGCACCTGGAGTTGCCGATCCCCATGGGACTTGGCAAAACTGCAAGCTTGACATAACGCATTGTAAACCCACTCAACTCAAAACCATGCAAGATTTCAGGGCGCAGTTTTTAAGTGCAATTGGTGGTGTGAGCGGCTCTCGATCAAAAGGAATGTTCATAGACTCTTGCTATGCACACTGCCAAACTGAACTGCAGGAGACATGGTTAATGCCCGACTCTCCAGTACTGAACAAGACGACGATTGCAAAGGCAGTTGGAGACTGGTTCTACGACCGAACTCCCTTTCAAAAGATTGATTGTCCTTACCCTTGCAATCCCACTTGCCATAACCGTAACTTTGATCCAAATCACCACCAACACAGTAATTTCGCCAAAATGTCGAGAAAGAAGGAATTTCCATGGCCGCTCCTGATTTTCGTGCACCTCCTTGGCTTTCCAAATTTCCATGGCTGCTGCTGGTCGTCGTTCGATTTTGGTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

422

Amino Acids

46.43

Weight (kDa)

9.08

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 5 - 370 1.6e-175 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 784
AccBSI CCGCTC 2 cut(s) 915, 1189
AccIII TCCGGA 2 cut(s) 280, 723
AciI CCGC 5 cut(s) 18, 53, 701, 915, 1187
AclWI GGATC 2 cut(s) 794, 1118
AcoI YGGCCR 1 cut(s) 1184
AcsI RAATTY 3 cut(s) 649, 1175, 1222
AcuI CTGAAG 1 cut(s) 364
AcyI GRCGYC 2 cut(s) 485, 728
AfaI GTAC 1 cut(s) 1008
AfiI CCNNNNNNNGG 1 cut(s) 109
AflIII ACRYGT 1 cut(s) 598
AgeI ACCGGT 1 cut(s) 368
AgsI TTSAA 4 cut(s) 413, 605, 672, 1070
AjnI CCWGG 2 cut(s) 682, 787
AjuI GAANNNNNNNTTGG 2 cut(s) 118, 150
AluBI AGCT 5 cut(s) 95, 353, 365, 533, 829
AluI AGCT 5 cut(s) 95, 353, 365, 533, 829
Alw21I GWGCWC 2 cut(s) 595, 1206
Alw26I GTCTC 2 cut(s) 974, 1035
Alw44I GTGCAC 1 cut(s) 1202
AlwI GGATC 2 cut(s) 794, 1118
AlwNI CAGNNNCTG 1 cut(s) 557
Aor13HI TCCGGA 2 cut(s) 280, 723
AoxI GGCC 1 cut(s) 1184
ApaLI GTGCAC 1 cut(s) 1202
ApeKI GCWGC 6 cut(s) 4, 14, 350, 749, 1233, 1236
ApoI RAATTY 3 cut(s) 649, 1175, 1222
AseI ATTAAT 1 cut(s) 570
AsiGI ACCGGT 1 cut(s) 368
AspLEI GCGC 1 cut(s) 886
AsuHPI GGTGA 2 cut(s) 353, 1124
BaeGI GKGCMC 1 cut(s) 1206
BanI GGYRCC 1 cut(s) 784
Bbv12I GWGCWC 2 cut(s) 595, 1206
BbvI GCAGC 5 cut(s) 26, 362, 761, 1220, 1223
BccI CCATC 3 cut(s) 74, 156, 667
BceAI ACGGC 1 cut(s) 53
BcgI CGANNNNNNTGC 2 cut(s) 634, 668
BciT130I CCWGG 2 cut(s) 684, 789
BcoDI GTCTC 2 cut(s) 974, 1035
BfaI CTAG 1 cut(s) 362
BfmI CTRYAG 1 cut(s) 974
BisI GCNGC 9 cut(s) 5, 15, 18, 351, 750, 916, 1187, 1234, 1237
BlsI GCNGC 9 cut(s) 6, 16, 19, 352, 751, 917, 1188, 1235, 1238
BmcAI AGTACT 1 cut(s) 1008
Bme1390I CCNGG 2 cut(s) 684, 789
BmiI GGNNCC 2 cut(s) 67, 786
BmrFI CCNGG 2 cut(s) 684, 789
BmsI GCATC 1 cut(s) 338
BplI GAGNNNNNCTC 2 cut(s) 904, 936
BpmI CTGGAG 3 cut(s) 609, 810, 987
BpuEI CTTGAG 1 cut(s) 215
BsaBI GATNNNNATC 1 cut(s) 1128
BsaHI GRCGYC 2 cut(s) 485, 728
BsaJI CCNNGG 4 cut(s) 805, 1181, 1210, 1228
BsaWI WCCGGW 3 cut(s) 280, 368, 723
BsaXI ACNNNNNCTCC 4 cut(s) 522, 552, 971, 1001
Bsc4I CCNNNNNNNGG 1 cut(s) 109
Bse118I RCCGGY 1 cut(s) 368
Bse1I ACTGG 4 cut(s) 299, 343, 1004, 1049
Bse8I GATNNNNATC 1 cut(s) 1128
BseAI TCCGGA 2 cut(s) 280, 723
BseBI CCWGG 2 cut(s) 684, 789
BseDI CCNNGG 4 cut(s) 805, 1181, 1210, 1228
BseGI GGATG 3 cut(s) 85, 167, 586
BseJI GATNNNNATC 1 cut(s) 1128
BseLI CCNNNNNNNGG 1 cut(s) 109
BseNI ACTGG 4 cut(s) 299, 343, 1004, 1049
BseRI GAGGAG 2 cut(s) 171, 399
BseSI GKGCMC 1 cut(s) 1206
BseXI GCAGC 5 cut(s) 26, 362, 761, 1220, 1223
BseYI CCCAGC 1 cut(s) 91
BsgI GTGCAG 1 cut(s) 423
Bsh1285I CGRYCG 1 cut(s) 1057
BshFI GGCC 1 cut(s) 1186
BshNI GGYRCC 1 cut(s) 784
BshTI ACCGGT 1 cut(s) 368
BsiEI CGRYCG 1 cut(s) 1057
BsiHKAI GWGCWC 2 cut(s) 595, 1206
BsiSI CCGG 3 cut(s) 281, 369, 724
BslFI GGGAC 2 cut(s) 72, 823
BslI CCNNNNNNNGG 1 cut(s) 109
BsmAI GTCTC 2 cut(s) 974, 1035
BsmFI GGGAC 2 cut(s) 72, 823
BsnI GGCC 1 cut(s) 1186
Bsp1286I GDGCHC 2 cut(s) 595, 1206
Bsp13I TCCGGA 2 cut(s) 280, 723
Bsp143I GATC 3 cut(s) 799, 923, 1123
Bsp19I CCATGG 3 cut(s) 805, 1181, 1228
BspACI CCGC 5 cut(s) 18, 53, 701, 915, 1187
BspANI GGCC 1 cut(s) 1186
BspEI TCCGGA 2 cut(s) 280, 723
BspLI GGNNCC 2 cut(s) 67, 786
BspMAI CTGCAG 1 cut(s) 978
BspPI GGATC 2 cut(s) 794, 1118
BspT107I GGYRCC 1 cut(s) 784
BsrBI CCGCTC 2 cut(s) 915, 1189
BsrFI RCCGGY 1 cut(s) 368
BsrI ACTGG 4 cut(s) 299, 343, 1004, 1049
BssAI RCCGGY 1 cut(s) 368
BssECI CCNNGG 4 cut(s) 805, 1181, 1210, 1228
BssMI GATC 3 cut(s) 799, 923, 1123
BssNI GRCGYC 2 cut(s) 485, 728
BssT1I CCWWGG 4 cut(s) 805, 1181, 1210, 1228
Bst2UI CCWGG 2 cut(s) 684, 789
Bst4CI ACNGT 5 cut(s) 206, 500, 609, 1115, 1145
BstACI GRCGYC 2 cut(s) 485, 728
BstC8I GCNNGC 2 cut(s) 266, 827
BstDEI CTNAG 1 cut(s) 219
BstDSI CCRYGG 3 cut(s) 805, 1181, 1228
BstENI CCTNNNNNAGG 1 cut(s) 107
BstF5I GGATG 3 cut(s) 85, 167, 586
BstHHI GCGC 1 cut(s) 886
BstKTI GATC 3 cut(s) 802, 926, 1126
BstMAI GTCTC 2 cut(s) 974, 1035
BstMBI GATC 3 cut(s) 799, 923, 1123
BstMCI CGRYCG 1 cut(s) 1057
BstMWI GCNNNNNNNGC 2 cut(s) 65, 449
BstNI CCWGG 2 cut(s) 684, 789
BstNSI RCATGY 1 cut(s) 602
BstSCI CCNGG 2 cut(s) 682, 787
BstSFI CTRYAG 1 cut(s) 974
BstSLI GKGCMC 1 cut(s) 1206
BstV1I GCAGC 5 cut(s) 26, 362, 761, 1220, 1223
BsuRI GGCC 1 cut(s) 1186
BtgI CCRYGG 3 cut(s) 805, 1181, 1228
BtsCI GGATG 3 cut(s) 85, 167, 586
BtsI GCAGTG 1 cut(s) 958
BtsIMutI CAGTG 3 cut(s) 336, 496, 958
Cac8I GCNNGC 2 cut(s) 266, 827
CaiI CAGNNNCTG 1 cut(s) 557
CfoI GCGC 1 cut(s) 886
Cfr10I RCCGGY 1 cut(s) 368
CseI GACGC 3 cut(s) 474, 563, 736
Csp6I GTAC 1 cut(s) 1007
CspAI ACCGGT 1 cut(s) 368
CviAII CATG 9 cut(s) 512, 599, 635, 762, 806, 868, 984, 1182, 1229
CviQI GTAC 1 cut(s) 1007
DdeI CTNAG 1 cut(s) 219
DpnI GATC 3 cut(s) 801, 925, 1125
DpnII GATC 3 cut(s) 799, 923, 1123
EaeI YGGCCR 1 cut(s) 1184
Eco130I CCWWGG 4 cut(s) 805, 1181, 1210, 1228
Eco57I CTGAAG 1 cut(s) 364
EcoNI CCTNNNNNAGG 1 cut(s) 107
EcoRII CCWGG 2 cut(s) 682, 787
EcoT14I CCWWGG 4 cut(s) 805, 1181, 1210, 1228
ErhI CCWWGG 4 cut(s) 805, 1181, 1210, 1228
FaeI CATG 9 cut(s) 515, 602, 638, 765, 809, 871, 987, 1185, 1232
FalI AAGNNNNNCTT 2 cut(s) 764, 796
FaqI GGGAC 2 cut(s) 72, 823
FatI CATG 9 cut(s) 511, 598, 634, 761, 805, 867, 983, 1181, 1228
FauI CCCGC 1 cut(s) 708
Fnu4HI GCNGC 9 cut(s) 5, 15, 18, 351, 750, 916, 1187, 1234, 1237
FokI GGATG 3 cut(s) 92, 174, 593
Fsp4HI GCNGC 9 cut(s) 5, 15, 18, 351, 750, 916, 1187, 1234, 1237
FspBI CTAG 1 cut(s) 362
GlaI GCGC 1 cut(s) 885
GluI GCNGC 9 cut(s) 5, 15, 18, 351, 750, 916, 1187, 1234, 1237
GsaI CCCAGC 1 cut(s) 95
GsuI CTGGAG 3 cut(s) 609, 810, 987
HaeIII GGCC 1 cut(s) 1186
HapII CCGG 3 cut(s) 281, 369, 724
HgaI GACGC 3 cut(s) 474, 563, 736
HhaI GCGC 1 cut(s) 886
Hin1I GRCGYC 2 cut(s) 485, 728
Hin1II CATG 9 cut(s) 515, 602, 638, 765, 809, 871, 987, 1185, 1232
Hin6I GCGC 1 cut(s) 884
HinP1I GCGC 1 cut(s) 884
HindIII AAGCTT 1 cut(s) 827
HinfI GANTC 7 cut(s) 85, 253, 303, 679, 758, 944, 998
HpaII CCGG 3 cut(s) 281, 369, 724
HphI GGTGA 2 cut(s) 353, 1124
Hpy166II GTNNAC 4 cut(s) 145, 358, 848, 1204
Hpy188I TCNGA 3 cut(s) 258, 383, 553
Hpy188III TCNNGA 7 cut(s) 123, 281, 588, 724, 921, 1164, 1192
Hpy8I GTNNAC 4 cut(s) 145, 358, 848, 1204
Hpy99I CGWCG 3 cut(s) 326, 1024, 1249
HpyAV CCTTC 3 cut(s) 433, 571, 1165
HpyCH4III ACNGT 5 cut(s) 206, 500, 609, 1115, 1145
HpyCH4IV ACGT 1 cut(s) 42
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 449
HpyF3I CTNAG 1 cut(s) 219
HpySE526I ACGT 1 cut(s) 42
Hsp92I GRCGYC 2 cut(s) 485, 728
Hsp92II CATG 9 cut(s) 515, 602, 638, 765, 809, 871, 987, 1185, 1232
HspAI GCGC 1 cut(s) 884
Kpn2I TCCGGA 2 cut(s) 280, 723
Kzo9I GATC 3 cut(s) 799, 923, 1123
LmnI GCTCC 5 cut(s) 65, 386, 530, 590, 1194
Lsp1109I GCAGC 5 cut(s) 26, 362, 761, 1220, 1223
LweI GCATC 1 cut(s) 338
MaeI CTAG 1 cut(s) 362
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 4 cut(s) 167, 500, 560, 1115
MalI GATC 3 cut(s) 801, 925, 1125
MbiI CCGCTC 2 cut(s) 915, 1189
MboI GATC 3 cut(s) 799, 923, 1123
MboII GAAGA 4 cut(s) 370, 425, 485, 658
MfeI CAATTG 2 cut(s) 133, 900
MhlI GDGCHC 2 cut(s) 595, 1206
MluCI AATT 9 cut(s) 126, 133, 237, 649, 674, 900, 1147, 1175, 1222
MlyI GAGTC 5 cut(s) 94, 312, 688, 938, 992
MmeI TCCRAC 2 cut(s) 508, 1018
MnlI CCTC 8 cut(s) 145, 149, 152, 377, 527, 666, 724, 1217
MroI TCCGGA 2 cut(s) 280, 723
MseI TTAA 5 cut(s) 129, 276, 570, 893, 989
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 3 cut(s) 281, 369, 724
MspR9I CCNGG 2 cut(s) 684, 789
MunI CAATTG 2 cut(s) 133, 900
MvaI CCWGG 2 cut(s) 684, 789
MwoI GCNNNNNNNGC 2 cut(s) 65, 449
NcoI CCATGG 3 cut(s) 805, 1181, 1228
NdeII GATC 3 cut(s) 799, 923, 1123
NlaIII CATG 9 cut(s) 515, 602, 638, 765, 809, 871, 987, 1185, 1232
NlaIV GGNNCC 2 cut(s) 67, 786
NmuCI GTSAC 1 cut(s) 500
NspI RCATGY 1 cut(s) 602
PciI ACATGT 1 cut(s) 598
PfeI GAWTC 2 cut(s) 253, 758
PinAI ACCGGT 1 cut(s) 368
PkrI GCNGC 9 cut(s) 6, 16, 19, 352, 751, 917, 1188, 1235, 1238
PleI GAGTC 5 cut(s) 93, 311, 687, 938, 992
PpsI GAGTC 5 cut(s) 93, 311, 687, 938, 992
PscI ACATGT 1 cut(s) 598
PshBI ATTAAT 1 cut(s) 570
Psp6I CCWGG 2 cut(s) 682, 787
PspFI CCCAGC 1 cut(s) 91
PspGI CCWGG 2 cut(s) 682, 787
PspN4I GGNNCC 2 cut(s) 67, 786
PstI CTGCAG 1 cut(s) 978
PstNI CAGNNNCTG 1 cut(s) 557
PvuII CAGCTG 1 cut(s) 353
RsaI GTAC 1 cut(s) 1008
RsaNI GTAC 1 cut(s) 1007
SaqAI TTAA 5 cut(s) 129, 276, 570, 893, 989
SatI GCNGC 9 cut(s) 5, 15, 18, 351, 750, 916, 1187, 1234, 1237
Sau3AI GATC 3 cut(s) 799, 923, 1123
ScaI AGTACT 1 cut(s) 1008
SchI GAGTC 5 cut(s) 94, 312, 688, 938, 992
ScrFI CCNGG 2 cut(s) 684, 789
SduI GDGCHC 2 cut(s) 595, 1206
SfaNI GCATC 1 cut(s) 338
SfcI CTRYAG 1 cut(s) 974
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
Sse9I AATT 9 cut(s) 126, 133, 237, 649, 674, 900, 1147, 1175, 1222
SsiI CCGC 5 cut(s) 18, 53, 701, 915, 1187
SspI AATATT 1 cut(s) 707
SspMI CTAG 1 cut(s) 362
StyD4I CCNGG 2 cut(s) 682, 787
StyI CCWWGG 4 cut(s) 805, 1181, 1210, 1228
TaaI ACNGT 5 cut(s) 206, 500, 609, 1115, 1145
TaiI ACGT 1 cut(s) 45
TaqI TCGA 3 cut(s) 922, 1163, 1251
TaqII GACCGA 1 cut(s) 1071
TasI AATT 9 cut(s) 126, 133, 237, 649, 674, 900, 1147, 1175, 1222
TatI WGTACW 1 cut(s) 1006
TauI GCSGC 3 cut(s) 20, 918, 1189
TfiI GAWTC 2 cut(s) 253, 758
Tru1I TTAA 5 cut(s) 129, 276, 570, 893, 989
Tru9I TTAA 5 cut(s) 129, 276, 570, 893, 989
TscAI CASTG 3 cut(s) 343, 503, 965
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 6 cut(s) 4, 14, 350, 749, 1233, 1236
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 5 cut(s) 35, 204, 556, 750, 928
TspGWI ACGGA 1 cut(s) 182
TspRI CASTG 3 cut(s) 343, 503, 965
VneI GTGCAC 1 cut(s) 1202
VspI ATTAAT 1 cut(s) 570
XagI CCTNNNNNAGG 1 cut(s) 107
XapI RAATTY 3 cut(s) 649, 1175, 1222
XceI RCATGY 1 cut(s) 602
XspI CTAG 1 cut(s) 362
ZrmI AGTACT 1 cut(s) 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.