Rw7G024390

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000996
Physical Location & Seq
Reverse (-)
29945671 .. 29949457
3787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G024390.1

Sequence Viewer

Length: 1188 bp
ATGGCCTATGCAAGAGTAGGCCAGTGGTTAAGTCTTCTGGTATGTTTACTGATATTGCTTAAAGCTGAAAGCCATCCTGTGGAACTCACCTTGGTCCAGAGTGCGGTTGCCAAAGGAGCTGTTTGTTTAGACGGGAGCCCACCAGCATACCACTTTGACAAGGGATTTGGTGCAGGGATTAATAATTGGTTGGTTCATATTGAGGGAGGAGGATGGTGTAAGGATGTTGAGAGTTGCGTTGAGCGAACGAAAAACTTCAGAGGTTCATCCACAAAAATGGAAAAGACAATATCTTTTTCTGGGGTTCTTGGTAAAAAGCAAACTATGAATCCAGACTTCTATAACTGGAACAGAATCAAGGTTAGGTACTGTGATGGGTCATCATTCACCGGTGATGTGGAAGCAGTAGACCCGGCTACAAATCTTCACTTCAGAGGAGGAAGGGTTTTTCGTGCCATCATTGATGATCTATTAGCAAAGGGAATGATAAATGCCAAAAATGCTCTACTCTCTGGTTGTTCGGCTGGAGGATTGGCTTCTATTCTTCAATGTGATAACTTCGGTTCTCTCTTACCCGCTGGAACTAATGTGAAATGCCTTGCGGATGCTGGTTACTTCATCAATGCGAAGACTATTACTGGGTCACAACAAATTGAAGCTTTTTATAGTCAAGTGGTTGCACTACATGGTTCAGCAAAGCATTTACCTGCATCCTGCACTTCAAGATTGAGACCAGGGTTGTGCTTCTTCCCACAAAATGTGATACCGCAACTCCGGACTCCTATTTTCTTGGTAAATGCTGCTTATGATTCGTGGCAGATAAAGAACATTTTGGCACCGAGTATTGCTGATCCTAAAGGCACATGGAAAAGCTGCAAGTCTGGTATAAAGAACTGCTCACCTGCGCAAATTCAAACCATGCAAGATTATAGGTTACAGTTCTTAGATGTACTCAATGGTGGTCTCACCAACTCTCCATCTCATGGAGCATTCATAGACTCTTGCTATGCTCACTGCCAAATCGGAACTCAAGAGACATGGATGGCATCTGACTCCCCGGTTGTCAGTAAGACGACAATTGCAAAGGCCGTCGGAGACTGGTATTACAACCGAAGTCCATCAAAAAAGATCGATTGTCCTTACCCTTGCAATCCTACCTGCAAAAATAAAGAATTTGATTCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

395

Amino Acids

42.96

Weight (kDa)

8.74

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 21 - 388 6.1e-175 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 910
Acc16I TGCGCA 1 cut(s) 906
Acc36I ACCTGC 3 cut(s) 715, 910, 1166
AccB1I GGYRCC 1 cut(s) 835
AccB7I CCANNNNNTGG 2 cut(s) 79, 983
AccI GTMKAC 1 cut(s) 408
AccIII TCCGGA 1 cut(s) 774
AciI CCGC 4 cut(s) 104, 576, 602, 767
AclWI GGATC 1 cut(s) 845
AcsI RAATTY 2 cut(s) 909, 1172
AcuI CTGAAG 2 cut(s) 241, 415
AfaI GTAC 2 cut(s) 368, 951
AfiI CCNNNNNNNGG 3 cut(s) 79, 103, 983
AgeI ACCGGT 1 cut(s) 389
AgsI TTSAA 5 cut(s) 548, 656, 723, 914, 1182
AjnI CCWGG 1 cut(s) 733
AluBI AGCT 4 cut(s) 65, 119, 659, 873
AluI AGCT 4 cut(s) 65, 119, 659, 873
Alw26I GTCTC 4 cut(s) 724, 968, 1028, 1089
AlwI GGATC 1 cut(s) 845
Aor13HI TCCGGA 1 cut(s) 774
AoxI GGCC 3 cut(s) 3, 19, 1086
ApeKI GCWGC 2 cut(s) 800, 873
ApoI RAATTY 2 cut(s) 909, 1172
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AseI ATTAAT 1 cut(s) 180
AsiGI ACCGGT 1 cut(s) 389
Asp700I GAANNNNTTC 1 cut(s) 254
AspLEI GCGC 1 cut(s) 907
AspS9I GGNCC 1 cut(s) 94
AsuC2I CCSGG 2 cut(s) 413, 1058
AsuHPI GGTGA 5 cut(s) 79, 379, 404, 891, 958
AvaII GGWCC 1 cut(s) 94
BanI GGYRCC 1 cut(s) 835
BanII GRGCYC 1 cut(s) 140
BbsI GAAGAC 2 cut(s) 26, 635
BbvI GCAGC 2 cut(s) 787, 860
BccI CCATC 7 cut(s) 81, 207, 368, 464, 985, 1036, 1126
BceAI ACGGC 1 cut(s) 1073
BciT130I CCWGG 1 cut(s) 735
BcnI CCSGG 2 cut(s) 413, 1058
BcoDI GTCTC 4 cut(s) 724, 968, 1028, 1089
BfaI CTAG 1 cut(s) 1186
BfuAI ACCTGC 3 cut(s) 715, 910, 1166
BisI GCNGC 2 cut(s) 801, 874
BlsI GCNGC 2 cut(s) 802, 875
Bme1390I CCNGG 3 cut(s) 413, 735, 1058
Bme18I GGWCC 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 94
BmiI GGNNCC 2 cut(s) 137, 837
BmrFI CCNGG 3 cut(s) 413, 735, 1058
BmrI ACTGGG 1 cut(s) 648
BmsI GCATC 3 cut(s) 595, 719, 1055
BmuI ACTGGG 1 cut(s) 648
BpiI GAAGAC 2 cut(s) 26, 635
BpmI CTGGAG 1 cut(s) 546
BpuEI CTTGAG 1 cut(s) 1014
BpuMI CCSGG 2 cut(s) 413, 1058
Bsa29I ATCGAT 1 cut(s) 1131
BsaI GGTCTC 2 cut(s) 724, 968
BsaJI CCNNGG 3 cut(s) 90, 734, 1056
BsaWI WCCGGW 2 cut(s) 389, 774
BsaXI ACNNNNNCTCC 6 cut(s) 429, 459, 756, 786, 958, 988
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 103, 983
Bse118I RCCGGY 1 cut(s) 389
Bse1I ACTGG 4 cut(s) 22, 350, 643, 1103
BseAI TCCGGA 1 cut(s) 774
BseBI CCWGG 1 cut(s) 735
BseCI ATCGAT 1 cut(s) 1131
BseDI CCNNGG 3 cut(s) 90, 734, 1056
BseGI GGATG 7 cut(s) 73, 218, 229, 266, 610, 710, 1047
BseLI CCNNNNNNNGG 3 cut(s) 79, 103, 983
BseNI ACTGG 4 cut(s) 22, 350, 643, 1103
BseRI GAGGAG 2 cut(s) 222, 450
BseXI GCAGC 2 cut(s) 787, 860
BsgI GTGCAG 2 cut(s) 192, 700
BshFI GGCC 3 cut(s) 5, 21, 1088
BshNI GGYRCC 1 cut(s) 835
BshTI ACCGGT 1 cut(s) 389
BshVI ATCGAT 1 cut(s) 1131
BsiSI CCGG 4 cut(s) 390, 413, 775, 1058
BslI CCNNNNNNNGG 3 cut(s) 79, 103, 983
BsmAI GTCTC 4 cut(s) 724, 968, 1028, 1089
BsmI GAATGC 1 cut(s) 989
BsnI GGCC 3 cut(s) 5, 21, 1088
Bso31I GGTCTC 2 cut(s) 724, 968
Bsp1286I GDGCHC 1 cut(s) 140
Bsp13I TCCGGA 1 cut(s) 774
Bsp143I GATC 3 cut(s) 466, 850, 1128
BspACI CCGC 4 cut(s) 104, 576, 602, 767
BspANI GGCC 3 cut(s) 5, 21, 1088
BspDI ATCGAT 1 cut(s) 1131
BspEI TCCGGA 1 cut(s) 774
BspLI GGNNCC 2 cut(s) 137, 837
BspMI ACCTGC 3 cut(s) 715, 910, 1166
BspPI GGATC 1 cut(s) 845
BspT107I GGYRCC 1 cut(s) 835
BspTNI GGTCTC 2 cut(s) 724, 968
BsrFI RCCGGY 1 cut(s) 389
BsrI ACTGG 4 cut(s) 22, 350, 643, 1103
BssAI RCCGGY 1 cut(s) 389
BssECI CCNNGG 3 cut(s) 90, 734, 1056
BssMI GATC 3 cut(s) 466, 850, 1128
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 735
Bst4CI ACNGT 2 cut(s) 371, 939
BstDEI CTNAG 1 cut(s) 943
BstF5I GGATG 7 cut(s) 73, 218, 229, 266, 610, 710, 1047
BstHHI GCGC 1 cut(s) 907
BstKTI GATC 3 cut(s) 469, 853, 1131
BstMAI GTCTC 4 cut(s) 724, 968, 1028, 1089
BstMBI GATC 3 cut(s) 466, 850, 1128
BstMWI GCNNNNNNNGC 2 cut(s) 116, 500
BstNI CCWGG 1 cut(s) 735
BstSCI CCNGG 3 cut(s) 411, 733, 1056
BstV1I GCAGC 2 cut(s) 787, 860
BstV2I GAAGAC 2 cut(s) 26, 635
BstXI CCANNNNNNTGG 1 cut(s) 277
Bsu15I ATCGAT 1 cut(s) 1131
BsuRI GGCC 3 cut(s) 5, 21, 1088
BsuTUI ATCGAT 1 cut(s) 1131
BtsCI GGATG 7 cut(s) 73, 218, 229, 266, 610, 710, 1047
BtsI GCAGTG 1 cut(s) 1012
BtsIMutI CAGTG 2 cut(s) 29, 1012
BveI ACCTGC 3 cut(s) 715, 910, 1166
CfoI GCGC 1 cut(s) 907
Cfr10I RCCGGY 1 cut(s) 389
Cfr13I GGNCC 1 cut(s) 94
ClaI ATCGAT 1 cut(s) 1131
Csp6I GTAC 2 cut(s) 367, 950
CspAI ACCGGT 1 cut(s) 389
CviAII CATG 5 cut(s) 686, 864, 919, 983, 1038
CviQI GTAC 2 cut(s) 367, 950
DdeI CTNAG 1 cut(s) 943
DpnI GATC 3 cut(s) 468, 852, 1130
DpnII GATC 3 cut(s) 466, 850, 1128
Eco130I CCWWGG 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 140
Eco31I GGTCTC 2 cut(s) 724, 968
Eco47I GGWCC 1 cut(s) 94
Eco57I CTGAAG 2 cut(s) 241, 415
EcoRII CCWGG 1 cut(s) 733
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 140
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 5 cut(s) 689, 867, 922, 986, 1041
FatI CATG 5 cut(s) 685, 863, 918, 982, 1037
FauI CCCGC 1 cut(s) 583
FblI GTMKAC 1 cut(s) 408
Fnu4HI GCNGC 2 cut(s) 801, 874
FokI GGATG 7 cut(s) 60, 225, 236, 253, 617, 697, 1054
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 2 cut(s) 801, 874
FspBI CTAG 1 cut(s) 1186
FspI TGCGCA 1 cut(s) 906
GlaI GCGC 1 cut(s) 906
GluI GCNGC 2 cut(s) 801, 874
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 3 cut(s) 5, 21, 1088
HapII CCGG 4 cut(s) 390, 413, 775, 1058
HhaI GCGC 1 cut(s) 907
Hin1II CATG 5 cut(s) 689, 867, 922, 986, 1041
Hin6I GCGC 1 cut(s) 905
HinP1I GCGC 1 cut(s) 905
HindIII AAGCTT 1 cut(s) 657
HinfI GANTC 7 cut(s) 328, 354, 778, 809, 998, 1052, 1178
HpaII CCGG 4 cut(s) 390, 413, 775, 1058
HphI GGTGA 5 cut(s) 79, 379, 404, 891, 958
Hpy166II GTNNAC 2 cut(s) 47, 409
Hpy188I TCNGA 5 cut(s) 260, 434, 1025, 1051, 1094
Hpy188III TCNNGA 5 cut(s) 97, 332, 723, 775, 1031
Hpy8I GTNNAC 2 cut(s) 47, 409
Hpy99I CGWCG 1 cut(s) 1094
HpyAV CCTTC 1 cut(s) 435
HpyCH4III ACNGT 2 cut(s) 371, 939
HpyF10VI GCNNNNNNNGC 2 cut(s) 116, 500
HpyF3I CTNAG 1 cut(s) 943
Hsp92II CATG 5 cut(s) 689, 867, 922, 986, 1041
HspAI GCGC 1 cut(s) 905
Kpn2I TCCGGA 1 cut(s) 774
Kzo9I GATC 3 cut(s) 466, 850, 1128
LmnI GCTCC 3 cut(s) 116, 135, 986
Lsp1109I GCAGC 2 cut(s) 787, 860
LweI GCATC 3 cut(s) 595, 719, 1055
MaeI CTAG 1 cut(s) 1186
MaeIII GTNAC 3 cut(s) 611, 642, 933
MalI GATC 3 cut(s) 468, 852, 1130
MboI GATC 3 cut(s) 466, 850, 1128
MboII GAAGA 5 cut(s) 26, 416, 536, 640, 739
MfeI CAATTG 1 cut(s) 1077
MhlI GDGCHC 1 cut(s) 140
MluCI AATT 5 cut(s) 184, 651, 909, 1077, 1172
MlyI GAGTC 3 cut(s) 772, 992, 1046
MmeI TCCRAC 1 cut(s) 1072
MnlI CCTC 7 cut(s) 196, 200, 203, 254, 428, 431, 521
MroI TCCGGA 1 cut(s) 774
MroXI GAANNNNTTC 1 cut(s) 254
MseI TTAA 3 cut(s) 29, 60, 180
MslI CAYNNNNRTG 1 cut(s) 275
MspA1I CMGCKG 1 cut(s) 578
MspI CCGG 4 cut(s) 390, 413, 775, 1058
MspR9I CCNGG 3 cut(s) 413, 735, 1058
MunI CAATTG 1 cut(s) 1077
Mva1269I GAATGC 1 cut(s) 989
MvaI CCWGG 1 cut(s) 735
MwoI GCNNNNNNNGC 2 cut(s) 116, 500
NciI CCSGG 2 cut(s) 413, 1058
NdeII GATC 3 cut(s) 466, 850, 1128
NlaIII CATG 5 cut(s) 689, 867, 922, 986, 1041
NlaIV GGNNCC 2 cut(s) 137, 837
NmuCI GTSAC 1 cut(s) 642
NsbI TGCGCA 1 cut(s) 906
PaqCI CACCTGC 1 cut(s) 910
PctI GAATGC 1 cut(s) 989
PdmI GAANNNNTTC 1 cut(s) 254
PfeI GAWTC 4 cut(s) 328, 354, 809, 1178
PflMI CCANNNNNTGG 2 cut(s) 79, 983
PinAI ACCGGT 1 cut(s) 389
PkrI GCNGC 2 cut(s) 802, 875
PleI GAGTC 3 cut(s) 772, 992, 1046
PpsI GAGTC 3 cut(s) 772, 992, 1046
PshBI ATTAAT 1 cut(s) 180
Psp6I CCWGG 1 cut(s) 733
PspGI CCWGG 1 cut(s) 733
PspN4I GGNNCC 2 cut(s) 137, 837
PspPI GGNCC 1 cut(s) 94
RsaI GTAC 2 cut(s) 368, 951
RsaNI GTAC 2 cut(s) 367, 950
RseI CAYNNNNRTG 1 cut(s) 275
SaqAI TTAA 3 cut(s) 29, 60, 180
SatI GCNGC 2 cut(s) 801, 874
Sau3AI GATC 3 cut(s) 466, 850, 1128
Sau96I GGNCC 1 cut(s) 94
SchI GAGTC 3 cut(s) 772, 992, 1046
ScrFI CCNGG 3 cut(s) 413, 735, 1058
SduI GDGCHC 1 cut(s) 140
SfaNI GCATC 3 cut(s) 595, 719, 1055
SgrAI CRCCGGYG 1 cut(s) 389
SinI GGWCC 1 cut(s) 94
SmiMI CAYNNNNRTG 1 cut(s) 275
SmlI CTYRAG 1 cut(s) 1029
SmoI CTYRAG 1 cut(s) 1029
Sse9I AATT 5 cut(s) 184, 651, 909, 1077, 1172
SsiI CCGC 4 cut(s) 104, 576, 602, 767
SspMI CTAG 1 cut(s) 1186
StyD4I CCNGG 3 cut(s) 411, 733, 1056
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 2 cut(s) 371, 939
TaqI TCGA 1 cut(s) 1131
TasI AATT 5 cut(s) 184, 651, 909, 1077, 1172
TatI WGTACW 1 cut(s) 949
TfiI GAWTC 4 cut(s) 328, 354, 809, 1178
Tru1I TTAA 3 cut(s) 29, 60, 180
Tru9I TTAA 3 cut(s) 29, 60, 180
TscAI CASTG 2 cut(s) 29, 1019
TseFI GTSAC 1 cut(s) 642
TseI GCWGC 2 cut(s) 800, 873
Tsp45I GTSAC 1 cut(s) 642
TspDTI ATGAA 5 cut(s) 185, 255, 341, 607, 982
TspRI CASTG 2 cut(s) 29, 1019
Van91I CCANNNNNTGG 2 cut(s) 79, 983
VpaK11BI GGWCC 1 cut(s) 94
VspI ATTAAT 1 cut(s) 180
XapI RAATTY 2 cut(s) 909, 1172
XmiI GTMKAC 1 cut(s) 408
XmnI GAANNNNTTC 1 cut(s) 254
XspI CTAG 1 cut(s) 1186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.