pycom06g01060

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000996
Physical Location & Seq
Forward (+)
1066001 .. 1069393
3393 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g01060.7

Sequence Viewer

Length: 1158 bp
ATGGTTCAGGAATTAACAATTGGTTGGTTCACATTGAGGTTTGTCCTTTTCTCTCAGCTCCCAGTACAGTTTCTTTCACAAGATAAGAGGAACAAAAAGAAGAAGGGAGGAGGATGGTGTAACAATATCACAACTTGTCTTGTCCGTAAAAACAACCGTTTAGGTTCATCTAAGAAAATGCTCAAGGAAATTGCATTTTCAGGGATTCTGAACGGCAGGCATATGTTTAATCCGGACTTTTACAACTGGAATAGAGTCAAAGTTAGGTATTGCGACGGGGCATCATTCACTGGTGATGTTGCAGCTGTAAACCTAGCCACCGGTCTTTTCTTCAGAGGAGCAAGGATTTTTCGTGCCGTCATTGAAGATCTATTAGCAAAAGGAATGGGGAAGGCTAAAAATGCTATTCTTTCTGGTTGTTCTGCTGGTGGATTGGCATCCATTCTTCACTGTGACAACTTCCATGCCCTCTTACCTGTTGGAGCTAAAGTGAAATGTGTTTCAGACGCTGGTTACTTCATTAATGCGAAGGATGTTTCTGGAGCACGACACGTTGAACAGTTTTTTAGTCAAGTGGTTGCAACACATGGTTCGGCGAAGAATTTGCCTCCATCTTGCACTTCAAAATTGAGTCCAGCTTTGTGCTTTTTCCCGCAATATTTTGCCTCACAAATCCGGACGCCAATGTTCTATGTAAATGCAGCCTACGATTCATGGCAGATAAAGAACATCTTGGCACCTGGAGTTGCCGATCCCCATGGGACTTGGCAGAACTGCAAGCTTGACATAACGCATTGTAAACCCACTCAACTCAAAACCATGCAAGATTTCAGGGCGCAGTTTTTAAGTGCAATTGGTGGTGTGAACGGCTCTCGATCAAAAGGAATGTTCATAGACTCTTGCTACGCACACTGCCAAACTGAACTGCAGGAGACATGGTTAATGCCCGACTCTCCAGTACTGAACAAGACGACGATTGCAAAGGCAGTTGGAGACTGGTTCTATGACCGAACTCCCTTTCAAAAGATTGATTGTCCTTACCCTTGTAATCCCACTTGCCATAACCGTAACTTTGATCCAAATCACCACCAACACAGTAATTTCGCCAAAATGTCGAGAAAGGTATATCAAACAAGGAATTTCCATGGCTGCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

386

Amino Acids

43.05

Weight (kDa)

9.49

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 736
AccIII TCCGGA 2 cut(s) 232, 675
AciI CCGC 1 cut(s) 653
AclWI GGATC 2 cut(s) 746, 1070
AcsI RAATTY 2 cut(s) 601, 1138
AcuI CTGAAG 1 cut(s) 316
AcyI GRCGYC 1 cut(s) 680
AfaI GTAC 2 cut(s) 66, 960
AflIII ACRYGT 1 cut(s) 550
AgeI ACCGGT 1 cut(s) 320
AgsI TTSAA 4 cut(s) 365, 557, 624, 1022
AjnI CCWGG 1 cut(s) 739
AjuI GAANNNNNNNTTGG 1 cut(s) 35
AluBI AGCT 5 cut(s) 58, 305, 485, 638, 781
AluI AGCT 5 cut(s) 58, 305, 485, 638, 781
Alw21I GWGCWC 1 cut(s) 547
Alw26I GTCTC 2 cut(s) 926, 987
AlwI GGATC 2 cut(s) 746, 1070
AlwNI CAGNNNCTG 1 cut(s) 509
Aor13HI TCCGGA 2 cut(s) 232, 675
ApeKI GCWGC 3 cut(s) 302, 701, 1149
ApoI RAATTY 2 cut(s) 601, 1138
AseI ATTAAT 1 cut(s) 522
AsiGI ACCGGT 1 cut(s) 320
AspLEI GCGC 1 cut(s) 838
AsuHPI GGTGA 2 cut(s) 305, 1076
BanI GGYRCC 1 cut(s) 736
Bbv12I GWGCWC 1 cut(s) 547
BbvI GCAGC 3 cut(s) 314, 713, 1136
BccI CCATC 2 cut(s) 108, 619
BceAI ACGGC 3 cut(s) 229, 341, 883
BcgI CGANNNNNNTGC 2 cut(s) 586, 620
BciT130I CCWGG 1 cut(s) 741
BcoDI GTCTC 2 cut(s) 926, 987
BfaI CTAG 1 cut(s) 314
BfmI CTRYAG 1 cut(s) 926
BglII AGATCT 1 cut(s) 367
BisI GCNGC 3 cut(s) 303, 702, 1150
BlsI GCNGC 3 cut(s) 304, 703, 1151
BmcAI AGTACT 1 cut(s) 960
Bme1390I CCNGG 1 cut(s) 741
BmiI GGNNCC 1 cut(s) 738
BmrFI CCNGG 1 cut(s) 741
BmrI ACTGGG 1 cut(s) 56
BmsI GCATC 2 cut(s) 290, 446
BmuI ACTGGG 1 cut(s) 56
BpmI CTGGAG 3 cut(s) 561, 762, 939
BpuEI CTTGAG 1 cut(s) 167
BsaBI GATNNNNATC 2 cut(s) 436, 1080
BsaHI GRCGYC 1 cut(s) 680
BsaJI CCNNGG 2 cut(s) 757, 1144
BsaWI WCCGGW 3 cut(s) 232, 320, 675
BsaXI ACNNNNNCTCC 4 cut(s) 474, 504, 923, 953
Bse118I RCCGGY 1 cut(s) 320
Bse1I ACTGG 5 cut(s) 62, 251, 295, 956, 1001
Bse8I GATNNNNATC 2 cut(s) 436, 1080
BseAI TCCGGA 2 cut(s) 232, 675
BseBI CCWGG 1 cut(s) 741
BseDI CCNNGG 2 cut(s) 757, 1144
BseGI GGATG 3 cut(s) 119, 437, 538
BseJI GATNNNNATC 2 cut(s) 436, 1080
BseMII CTCAG 1 cut(s) 68
BseNI ACTGG 5 cut(s) 62, 251, 295, 956, 1001
BseRI GAGGAG 2 cut(s) 123, 351
BseXI GCAGC 3 cut(s) 314, 713, 1136
BshNI GGYRCC 1 cut(s) 736
BshTI ACCGGT 1 cut(s) 320
BsiHKAI GWGCWC 1 cut(s) 547
BsiSI CCGG 3 cut(s) 233, 321, 676
BslFI GGGAC 1 cut(s) 775
BsmAI GTCTC 2 cut(s) 926, 987
BsmFI GGGAC 1 cut(s) 775
Bsp1286I GDGCHC 1 cut(s) 547
Bsp13I TCCGGA 2 cut(s) 232, 675
Bsp143I GATC 4 cut(s) 367, 751, 875, 1075
Bsp19I CCATGG 2 cut(s) 757, 1144
BspACI CCGC 1 cut(s) 653
BspCNI CTCAG 1 cut(s) 67
BspEI TCCGGA 2 cut(s) 232, 675
BspLI GGNNCC 1 cut(s) 738
BspMAI CTGCAG 1 cut(s) 930
BspPI GGATC 2 cut(s) 746, 1070
BspT107I GGYRCC 1 cut(s) 736
BsrFI RCCGGY 1 cut(s) 320
BsrI ACTGG 5 cut(s) 62, 251, 295, 956, 1001
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 2 cut(s) 757, 1144
BssMI GATC 4 cut(s) 367, 751, 875, 1075
BssNI GRCGYC 1 cut(s) 680
BssT1I CCWWGG 2 cut(s) 757, 1144
Bst2UI CCWGG 1 cut(s) 741
Bst4CI ACNGT 6 cut(s) 69, 158, 452, 561, 1067, 1097
BstACI GRCGYC 1 cut(s) 680
BstC8I GCNNGC 2 cut(s) 218, 779
BstDEI CTNAG 2 cut(s) 54, 171
BstDSI CCRYGG 2 cut(s) 757, 1144
BstF5I GGATG 3 cut(s) 119, 437, 538
BstHHI GCGC 1 cut(s) 838
BstKTI GATC 4 cut(s) 370, 754, 878, 1078
BstMAI GTCTC 2 cut(s) 926, 987
BstMBI GATC 4 cut(s) 367, 751, 875, 1075
BstMWI GCNNNNNNNGC 1 cut(s) 401
BstNI CCWGG 1 cut(s) 741
BstSCI CCNGG 1 cut(s) 739
BstSFI CTRYAG 1 cut(s) 926
BstV1I GCAGC 3 cut(s) 314, 713, 1136
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
BtgI CCRYGG 2 cut(s) 757, 1144
BtsCI GGATG 3 cut(s) 119, 437, 538
BtsI GCAGTG 1 cut(s) 910
BtsIMutI CAGTG 3 cut(s) 288, 448, 910
Cac8I GCNNGC 2 cut(s) 218, 779
CaiI CAGNNNCTG 1 cut(s) 509
CfoI GCGC 1 cut(s) 838
Cfr10I RCCGGY 1 cut(s) 320
CseI GACGC 2 cut(s) 515, 688
Csp6I GTAC 2 cut(s) 65, 959
CspAI ACCGGT 1 cut(s) 320
CviAII CATG 7 cut(s) 464, 587, 714, 758, 820, 936, 1145
CviQI GTAC 2 cut(s) 65, 959
DdeI CTNAG 2 cut(s) 54, 171
DpnI GATC 4 cut(s) 369, 753, 877, 1077
DpnII GATC 4 cut(s) 367, 751, 875, 1075
Eco130I CCWWGG 2 cut(s) 757, 1144
Eco57I CTGAAG 1 cut(s) 316
EcoRII CCWGG 1 cut(s) 739
EcoT14I CCWWGG 2 cut(s) 757, 1144
ErhI CCWWGG 2 cut(s) 757, 1144
FaeI CATG 7 cut(s) 467, 590, 717, 761, 823, 939, 1148
FalI AAGNNNNNCTT 2 cut(s) 716, 748
FaqI GGGAC 1 cut(s) 775
FatI CATG 7 cut(s) 463, 586, 713, 757, 819, 935, 1144
FauI CCCGC 1 cut(s) 660
FauNDI CATATG 1 cut(s) 222
Fnu4HI GCNGC 3 cut(s) 303, 702, 1150
FokI GGATG 3 cut(s) 126, 424, 545
Fsp4HI GCNGC 3 cut(s) 303, 702, 1150
FspBI CTAG 1 cut(s) 314
GlaI GCGC 1 cut(s) 837
GluI GCNGC 3 cut(s) 303, 702, 1150
GsuI CTGGAG 3 cut(s) 561, 762, 939
HapII CCGG 3 cut(s) 233, 321, 676
HgaI GACGC 2 cut(s) 515, 688
HhaI GCGC 1 cut(s) 838
Hin1I GRCGYC 1 cut(s) 680
Hin1II CATG 7 cut(s) 467, 590, 717, 761, 823, 939, 1148
Hin6I GCGC 1 cut(s) 836
HinP1I GCGC 1 cut(s) 836
HindIII AAGCTT 1 cut(s) 779
HinfI GANTC 6 cut(s) 205, 255, 631, 710, 896, 950
HpaII CCGG 3 cut(s) 233, 321, 676
HphI GGTGA 2 cut(s) 305, 1076
Hpy166II GTNNAC 4 cut(s) 30, 310, 800, 865
Hpy188I TCNGA 3 cut(s) 210, 335, 505
Hpy188III TCNNGA 7 cut(s) 8, 233, 540, 676, 873, 1116, 1155
Hpy8I GTNNAC 4 cut(s) 30, 310, 800, 865
Hpy99I CGWCG 2 cut(s) 278, 976
HpyAV CCTTC 3 cut(s) 97, 385, 523
HpyCH4III ACNGT 6 cut(s) 69, 158, 452, 561, 1067, 1097
HpyCH4IV ACGT 1 cut(s) 552
HpyF10VI GCNNNNNNNGC 1 cut(s) 401
HpyF3I CTNAG 2 cut(s) 54, 171
HpySE526I ACGT 1 cut(s) 552
Hsp92I GRCGYC 1 cut(s) 680
Hsp92II CATG 7 cut(s) 467, 590, 717, 761, 823, 939, 1148
HspAI GCGC 1 cut(s) 836
Kpn2I TCCGGA 2 cut(s) 232, 675
Kzo9I GATC 4 cut(s) 367, 751, 875, 1075
LmnI GCTCC 5 cut(s) 63, 338, 482, 542, 1157
Lsp1109I GCAGC 3 cut(s) 314, 713, 1136
LweI GCATC 2 cut(s) 290, 446
MaeI CTAG 1 cut(s) 314
MaeII ACGT 1 cut(s) 552
MaeIII GTNAC 4 cut(s) 119, 452, 512, 1067
MalI GATC 4 cut(s) 369, 753, 877, 1077
MboI GATC 4 cut(s) 367, 751, 875, 1075
MboII GAAGA 5 cut(s) 112, 322, 377, 437, 610
MfeI CAATTG 2 cut(s) 18, 852
MflI RGATCY 1 cut(s) 367
MhlI GDGCHC 1 cut(s) 547
MluCI AATT 8 cut(s) 11, 18, 189, 601, 626, 852, 1099, 1138
MlyI GAGTC 4 cut(s) 264, 640, 890, 944
MmeI TCCRAC 2 cut(s) 460, 970
MnlI CCTC 8 cut(s) 30, 81, 101, 104, 329, 479, 618, 676
MroI TCCGGA 2 cut(s) 232, 675
MseI TTAA 5 cut(s) 14, 228, 522, 845, 941
MspA1I CMGCKG 1 cut(s) 305
MspI CCGG 3 cut(s) 233, 321, 676
MspR9I CCNGG 1 cut(s) 741
MunI CAATTG 2 cut(s) 18, 852
MvaI CCWGG 1 cut(s) 741
MwoI GCNNNNNNNGC 1 cut(s) 401
NcoI CCATGG 2 cut(s) 757, 1144
NdeI CATATG 1 cut(s) 222
NdeII GATC 4 cut(s) 367, 751, 875, 1075
NlaIII CATG 7 cut(s) 467, 590, 717, 761, 823, 939, 1148
NlaIV GGNNCC 1 cut(s) 738
NmuCI GTSAC 1 cut(s) 452
PfeI GAWTC 2 cut(s) 205, 710
PinAI ACCGGT 1 cut(s) 320
PkrI GCNGC 3 cut(s) 304, 703, 1151
PleI GAGTC 4 cut(s) 263, 639, 890, 944
PpsI GAGTC 4 cut(s) 263, 639, 890, 944
PshBI ATTAAT 1 cut(s) 522
Psp6I CCWGG 1 cut(s) 739
PspGI CCWGG 1 cut(s) 739
PspN4I GGNNCC 1 cut(s) 738
PstI CTGCAG 1 cut(s) 930
PstNI CAGNNNCTG 1 cut(s) 509
PsuI RGATCY 1 cut(s) 367
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 2 cut(s) 66, 960
RsaNI GTAC 2 cut(s) 65, 959
SaqAI TTAA 5 cut(s) 14, 228, 522, 845, 941
SatI GCNGC 3 cut(s) 303, 702, 1150
Sau3AI GATC 4 cut(s) 367, 751, 875, 1075
ScaI AGTACT 1 cut(s) 960
SchI GAGTC 4 cut(s) 264, 640, 890, 944
ScrFI CCNGG 1 cut(s) 741
SduI GDGCHC 1 cut(s) 547
SfaNI GCATC 2 cut(s) 290, 446
SfcI CTRYAG 1 cut(s) 926
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
Sse9I AATT 8 cut(s) 11, 18, 189, 601, 626, 852, 1099, 1138
SsiI CCGC 1 cut(s) 653
SspI AATATT 1 cut(s) 659
SspMI CTAG 1 cut(s) 314
StyD4I CCNGG 1 cut(s) 739
StyI CCWWGG 2 cut(s) 757, 1144
TaaI ACNGT 6 cut(s) 69, 158, 452, 561, 1067, 1097
TaiI ACGT 1 cut(s) 555
TaqI TCGA 2 cut(s) 874, 1115
TaqII GACCGA 1 cut(s) 1023
TasI AATT 8 cut(s) 11, 18, 189, 601, 626, 852, 1099, 1138
TatI WGTACW 2 cut(s) 64, 958
TfiI GAWTC 2 cut(s) 205, 710
Tru1I TTAA 5 cut(s) 14, 228, 522, 845, 941
Tru9I TTAA 5 cut(s) 14, 228, 522, 845, 941
TscAI CASTG 3 cut(s) 295, 455, 917
TseFI GTSAC 1 cut(s) 452
TseI GCWGC 3 cut(s) 302, 701, 1149
Tsp45I GTSAC 1 cut(s) 452
TspDTI ATGAA 4 cut(s) 156, 508, 702, 880
TspGWI ACGGA 1 cut(s) 134
TspRI CASTG 3 cut(s) 295, 455, 917
VspI ATTAAT 1 cut(s) 522
XapI RAATTY 2 cut(s) 601, 1138
XspI CTAG 1 cut(s) 314
ZrmI AGTACT 1 cut(s) 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.