Rorug07G0149000

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000996
Physical Location & Seq
Forward (+)
11804055 .. 11804606
552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0149000.1

Sequence Viewer

Length: 552 bp
ATGTCAACTATAGTAGCAAAAAGTTCCATTCTTTTGAGGACCTTCATGTTGGTGTTCCCATCTGTTTTGGCAACCAAAAATCCATTCGAAGAGTCGAAACCATGCAAGAGGCTTGTGCTCTATTACCACGACACCCTATTTAACGGCACGGATGCGACCAATGCAACATCTGCTACTGTAGCCAATATAACAGGGCTTGACAGAGAGCACAAGTTTGGTATGCTGGTTGTCTTCAACGATCCTCTGACCAAAGACCACCATCTTCTGTCTCCTGCCGTCGGAAGAGCTCAAGGCTTCTATTTCTATGACATGAAGGACGACTACACTGCTTGGTTTGCCTACACTTTGGTGTTCAACTCGAGCGAGCACAAGGGTACACTCAATATCATGGGGGCGGATTTGATGTATGAAAAGACTAGGGATCTTTCGGTTGTCGGAGGGACGGGAGATTTCTTCATGGCTAGAGGGATTTGCACATTTCAAACTGATACTTTTCAGGGTGACTACTATTTTCGCCTTAAGATGGATATCAAGTTGTATGAATGTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

183

Amino Acids

20.84

Weight (kDa)

6.18

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 35 - 177 2.6e-38 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 395
AclWI GGATC 2 cut(s) 233, 429
AfaI GTAC 1 cut(s) 376
AfiI CCNNNNNNNGG 2 cut(s) 278, 523
AflII CTTAAG 1 cut(s) 518
AgsI TTSAA 3 cut(s) 235, 355, 482
AjuI GAANNNNNNNTTGG 2 cut(s) 68, 100
AleI CACNNNNGTG 1 cut(s) 347
AluBI AGCT 1 cut(s) 287
AluI AGCT 1 cut(s) 287
Alw21I GWGCWC 4 cut(s) 120, 210, 289, 369
Alw26I GTCTC 1 cut(s) 273
AlwI GGATC 2 cut(s) 233, 429
Ama87I CYCGRG 1 cut(s) 358
AspS9I GGNCC 1 cut(s) 39
AsuHPI GGTGA 1 cut(s) 512
AsuII TTCGAA 1 cut(s) 87
AvaI CYCGRG 1 cut(s) 358
AvaII GGWCC 1 cut(s) 39
BanII GRGCYC 1 cut(s) 289
BarI GAAGNNNNNNTAC 2 cut(s) 305, 337
BbsI GAAGAC 1 cut(s) 223
Bbv12I GWGCWC 4 cut(s) 120, 210, 289, 369
BccI CCATC 3 cut(s) 67, 267, 517
BceAI ACGGC 2 cut(s) 160, 260
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BcoDI GTCTC 1 cut(s) 273
BfaI CTAG 2 cut(s) 417, 462
BfmI CTRYAG 2 cut(s) 9, 177
BfrI CTTAAG 1 cut(s) 518
Bme18I GGWCC 1 cut(s) 39
BmeT110I CYCGRG 1 cut(s) 358
BmgT120I GGNCC 1 cut(s) 39
BmsI GCATC 1 cut(s) 142
BpiI GAAGAC 1 cut(s) 223
Bpu14I TTCGAA 1 cut(s) 87
BpuEI CTTGAG 1 cut(s) 273
BsaBI GATNNNNATC 1 cut(s) 527
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 523
Bse8I GATNNNNATC 1 cut(s) 527
BseGI GGATG 1 cut(s) 157
BseJI GATNNNNATC 1 cut(s) 527
BseLI CCNNNNNNNGG 2 cut(s) 278, 523
BsiHKAI GWGCWC 4 cut(s) 120, 210, 289, 369
BsiHKCI CYCGRG 1 cut(s) 358
BslFI GGGAC 1 cut(s) 454
BslI CCNNNNNNNGG 2 cut(s) 278, 523
BsmAI GTCTC 1 cut(s) 273
BsmFI GGGAC 1 cut(s) 454
BsoBI CYCGRG 1 cut(s) 358
Bsp119I TTCGAA 1 cut(s) 87
Bsp1286I GDGCHC 4 cut(s) 120, 210, 289, 369
Bsp143I GATC 2 cut(s) 238, 421
BspACI CCGC 1 cut(s) 395
BspPI GGATC 2 cut(s) 233, 429
BspQI GCTCTTC 1 cut(s) 277
BspT104I TTCGAA 1 cut(s) 87
BspTI CTTAAG 1 cut(s) 518
BssMI GATC 2 cut(s) 238, 421
Bst4CI ACNGT 1 cut(s) 178
Bst6I CTCTTC 2 cut(s) 84, 277
BstAFI CTTAAG 1 cut(s) 518
BstAPI GCANNNNNTGC 1 cut(s) 170
BstBI TTCGAA 1 cut(s) 87
BstC8I GCNNGC 1 cut(s) 365
BstF5I GGATG 1 cut(s) 157
BstKTI GATC 2 cut(s) 241, 424
BstMAI GTCTC 1 cut(s) 273
BstMBI GATC 2 cut(s) 238, 421
BstMWI GCNNNNNNNGC 4 cut(s) 161, 170, 179, 335
BstSFI CTRYAG 2 cut(s) 9, 177
BstV2I GAAGAC 1 cut(s) 223
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
BtsCI GGATG 1 cut(s) 157
BtsI GCAGTG 1 cut(s) 324
BtsIMutI CAGTG 1 cut(s) 324
Cac8I GCNNGC 1 cut(s) 365
Cfr13I GGNCC 1 cut(s) 39
Csp6I GTAC 1 cut(s) 375
CviAII CATG 5 cut(s) 46, 102, 310, 388, 457
CviJI RGCY 6 cut(s) 112, 182, 196, 287, 294, 461
CviKI_1 RGCY 6 cut(s) 112, 182, 196, 287, 294, 461
CviQI GTAC 1 cut(s) 375
DpnI GATC 2 cut(s) 240, 423
DpnII GATC 2 cut(s) 238, 421
Eam1104I CTCTTC 2 cut(s) 84, 277
EarI CTCTTC 2 cut(s) 84, 277
EciI GGCGGA 1 cut(s) 410
Ecl136II GAGCTC 1 cut(s) 287
Eco24I GRGCYC 1 cut(s) 289
Eco32I GATATC 1 cut(s) 529
Eco47I GGWCC 1 cut(s) 39
Eco53kI GAGCTC 1 cut(s) 287
Eco88I CYCGRG 1 cut(s) 358
EcoICRI GAGCTC 1 cut(s) 287
EcoO109I RGGNCCY 1 cut(s) 39
EcoRV GATATC 1 cut(s) 529
EcoT38I GRGCYC 1 cut(s) 289
FaeI CATG 5 cut(s) 49, 105, 313, 391, 460
FaqI GGGAC 1 cut(s) 454
FatI CATG 5 cut(s) 45, 101, 309, 387, 456
FokI GGATG 1 cut(s) 164
FriOI GRGCYC 1 cut(s) 289
FspBI CTAG 2 cut(s) 417, 462
Hin1II CATG 5 cut(s) 49, 105, 313, 391, 460
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 1 cut(s) 92
HphI GGTGA 1 cut(s) 512
Hpy166II GTNNAC 2 cut(s) 6, 377
Hpy188I TCNGA 3 cut(s) 246, 281, 437
Hpy8I GTNNAC 2 cut(s) 6, 377
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 2 cut(s) 52, 307
HpyCH4III ACNGT 1 cut(s) 178
HpyCH4V TGCA 3 cut(s) 105, 164, 474
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 170, 179, 335
Hsp92II CATG 5 cut(s) 49, 105, 313, 391, 460
Kzo9I GATC 2 cut(s) 238, 421
LguI GCTCTTC 1 cut(s) 277
LpnPI CCDG 4 cut(s) 177, 209, 285, 482
LweI GCATC 1 cut(s) 142
MaeI CTAG 2 cut(s) 417, 462
MaeIII GTNAC 1 cut(s) 500
MalI GATC 2 cut(s) 240, 423
MboI GATC 2 cut(s) 238, 421
MboII GAAGA 5 cut(s) 101, 223, 254, 294, 445
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 4 cut(s) 120, 210, 289, 369
MlyI GAGTC 1 cut(s) 101
MmeI TCCRAC 2 cut(s) 259, 415
MnlI CCTC 5 cut(s) 30, 102, 252, 431, 458
MseI TTAA 2 cut(s) 141, 519
MslI CAYNNNNRTG 2 cut(s) 50, 347
MspCI CTTAAG 1 cut(s) 518
MwoI GCNNNNNNNGC 4 cut(s) 161, 170, 179, 335
NdeII GATC 2 cut(s) 238, 421
NlaIII CATG 5 cut(s) 49, 105, 313, 391, 460
NmuCI GTSAC 1 cut(s) 500
NspV TTCGAA 1 cut(s) 87
OliI CACNNNNGTG 1 cut(s) 347
PaeR7I CTCGAG 1 cut(s) 358
PciSI GCTCTTC 1 cut(s) 277
PleI GAGTC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 100
PpuMI RGGWCCY 1 cut(s) 39
Psp124BI GAGCTC 1 cut(s) 289
Psp5II RGGWCCY 1 cut(s) 39
PspPI GGNCC 1 cut(s) 39
PspPPI RGGWCCY 1 cut(s) 39
PspXI VCTCGAGB 1 cut(s) 358
PsuI RGATCY 1 cut(s) 421
RsaI GTAC 1 cut(s) 376
RsaNI GTAC 1 cut(s) 375
RseI CAYNNNNRTG 2 cut(s) 50, 347
SacI GAGCTC 1 cut(s) 289
SapI GCTCTTC 1 cut(s) 277
SaqAI TTAA 2 cut(s) 141, 519
Sau3AI GATC 2 cut(s) 238, 421
Sau96I GGNCC 1 cut(s) 39
SchI GAGTC 1 cut(s) 101
SduI GDGCHC 4 cut(s) 120, 210, 289, 369
SetI ASST 2 cut(s) 44, 289
SfaNI GCATC 1 cut(s) 142
SfcI CTRYAG 2 cut(s) 9, 177
Sfr274I CTCGAG 1 cut(s) 358
SfuI TTCGAA 1 cut(s) 87
SinI GGWCC 1 cut(s) 39
SlaI CTCGAG 1 cut(s) 358
SmiMI CAYNNNNRTG 2 cut(s) 50, 347
SmlI CTYRAG 3 cut(s) 288, 358, 518
SmoI CTYRAG 3 cut(s) 288, 358, 518
SsiI CCGC 1 cut(s) 395
SspMI CTAG 2 cut(s) 417, 462
SstI GAGCTC 1 cut(s) 289
TaaI ACNGT 1 cut(s) 178
TaqI TCGA 3 cut(s) 87, 95, 359
Tru1I TTAA 2 cut(s) 141, 519
Tru9I TTAA 2 cut(s) 141, 519
TscAI CASTG 1 cut(s) 331
TseFI GTSAC 1 cut(s) 500
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 4 cut(s) 34, 326, 423, 445
TspGWI ACGGA 1 cut(s) 164
TspRI CASTG 1 cut(s) 331
Vha464I CTTAAG 1 cut(s) 518
VpaK11BI GGWCC 1 cut(s) 39
XhoI CTCGAG 1 cut(s) 358
XspI CTAG 2 cut(s) 417, 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.