FvH4_5g24510

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000996
Physical Location & Seq
Reverse (-)
15859156 .. 15863484
4329 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g24510.t3

Sequence Viewer

Length: 1200 bp
ATGGGTGATGTAAGATTAGGCCAATGGTTGAATCTTCTAGTATGTTCTGCGCTACTACTAAAAGCAACAGGAGTTCCTGTGGGGATCACTATCCTTCAGACTGCGGTTGCCAAAGGAGCCGTTTGTTTGGATGGGAGCCCCCCAGCTTACCATTGGGATAAGGGATTTGGTTCAGGACTTAACAATTGGTTGGTTCACATTGAGGGAGGAGGATGGTGTCACAATGTCACAAATTGCCTCGGCCGTAAGAACACTCGTTTAGGTTCATCAAAGAAAATGCTGAAGGAAATACCGTTTTCTGGAATTCTAAGCAACAGGCAATCGTTAAATCCAGGCTTTTACAATTGGAATAGAATCAAAGTCAGGTACTGCGATGGTTCATCATTCACTGGTGATGTTGCGGCAGTAGACCCGGCTACTGGTCTTTACTTCAGAGGAGCAAGGGTTTTCCGCGCTGTCATGGCTGATTTATTAGCAAAAGGAATGGGGAAAGCAGTAAATGCTGTTCTCTCTGGTTGTTCTGCTGGTGGATTGACCTCTATTCTTCATTGTGATAACTTTCGTGCTCTCTTCTTACCTTCTGGGACCAAAGTGAAATGTGTTTCAGATGCTGGTTACTTTATTAATGTAAAGGACATTTCTGGAGCACAACATGTTCAAGAGTATTTTACCGATATTGTTACAACACATGGTTCTGCGAAGAATTTGCCTCCATCTTGCACTTCAAAATTGAGACCAGATTTGTGTTTCTTCCCTCAATATGTGGTACCACAGATTCGGACACCAATATTCTATGTAAATGCAGCCTATGATTCATGGCAGATAAAGAACATTGTCGTACCAGGAGTTGCTGATCCTCATGGAACTTGGCGAAACTGCAAGCTTGATATAAAGAATTGCTCACCCACTCAGCTCAAAACCATGCAAAACTTCAGGTTGCAATTCTTAAGTGCAATTGGTGGTGTGAGCAACTCTGCAGTAAAAGGAATGTTCATAGACTCTTGCTATCTGCACTGTCAAACTGAATTGCAGGAGACTTGGTCAATGCCTAATTCCCCACTCCTCAACAAAACGACAATTGCAAGGGCAGTTGGAGACTGGTTCTACGACCGAACTTCGTTCCAAAAGATAGACTGTCCTTACCCTTGCAATCCCACTTGCCATGCTCCCATTTTTGATCCAAATGTGCGCTTAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

400

Amino Acids

43.73

Weight (kDa)

9.02

Isoelectric Point (pI)

38.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 22 - 358 1.7e-155 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 766
AccB1I GGYRCC 1 cut(s) 766
AccI GTMKAC 1 cut(s) 408
AccII CGCG 1 cut(s) 453
AciI CCGC 3 cut(s) 104, 401, 451
AclWI GGATC 3 cut(s) 92, 848, 1172
AcoI YGGCCR 1 cut(s) 241
AcsI RAATTY 2 cut(s) 303, 703
AcuI CTGAAG 4 cut(s) 80, 302, 415, 916
AfaI GTAC 3 cut(s) 368, 768, 840
AfiI CCNNNNNNNGG 2 cut(s) 299, 419
AflII CTTAAG 1 cut(s) 946
AflIII ACRYGT 1 cut(s) 652
AgsI TTSAA 3 cut(s) 31, 659, 726
AjnI CCWGG 2 cut(s) 331, 841
AluBI AGCT 3 cut(s) 146, 883, 913
AluI AGCT 3 cut(s) 146, 883, 913
Alw21I GWGCWC 2 cut(s) 568, 649
Alw26I GTCTC 3 cut(s) 727, 1028, 1089
AlwI GGATC 3 cut(s) 92, 848, 1172
AlwNI CAGNNNCTG 2 cut(s) 369, 611
AoxI GGCC 2 cut(s) 19, 241
ApeKI GCWGC 1 cut(s) 803
ApoI RAATTY 2 cut(s) 303, 703
AseI ATTAAT 1 cut(s) 624
Asp718I GGTACC 1 cut(s) 766
AspLEI GCGC 3 cut(s) 52, 455, 1191
AspS9I GGNCC 1 cut(s) 585
AsuC2I CCSGG 1 cut(s) 413
AsuHPI GGTGA 3 cut(s) 17, 404, 894
AvaII GGWCC 1 cut(s) 585
BanI GGYRCC 1 cut(s) 766
BanII GRGCYC 1 cut(s) 140
Bbv12I GWGCWC 2 cut(s) 568, 649
BbvI GCAGC 1 cut(s) 815
BccI CCATC 4 cut(s) 125, 207, 368, 721
BceAI ACGGC 2 cut(s) 104, 228
BcgI CGANNNNNNTGC 2 cut(s) 688, 722
BciT130I CCWGG 2 cut(s) 333, 843
BcnI CCSGG 1 cut(s) 413
BcoDI GTCTC 3 cut(s) 727, 1028, 1089
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 1 cut(s) 975
BfrI CTTAAG 1 cut(s) 946
BisI GCNGC 2 cut(s) 402, 804
BlsI GCNGC 2 cut(s) 403, 805
Bme1390I CCNGG 3 cut(s) 333, 413, 843
Bme18I GGWCC 1 cut(s) 585
BmgT120I GGNCC 1 cut(s) 585
BmiI GGNNCC 4 cut(s) 118, 137, 586, 768
BmrFI CCNGG 3 cut(s) 333, 413, 843
BmsI GCATC 1 cut(s) 598
BpmI CTGGAG 1 cut(s) 663
BpuMI CCSGG 1 cut(s) 413
BsaBI GATNNNNATC 1 cut(s) 89
BsaI GGTCTC 1 cut(s) 727
BsaJI CCNNGG 1 cut(s) 238
BsaXI ACNNNNNCTCC 6 cut(s) 63, 93, 429, 459, 1025, 1055
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 419
Bse1I ACTGG 3 cut(s) 394, 424, 1103
Bse8I GATNNNNATC 1 cut(s) 89
BseBI CCWGG 2 cut(s) 333, 843
BseDI CCNNGG 1 cut(s) 238
BseGI GGATG 2 cut(s) 136, 218
BseJI GATNNNNATC 1 cut(s) 89
BseLI CCNNNNNNNGG 2 cut(s) 299, 419
BseMII CTCAG 1 cut(s) 923
BseNI ACTGG 3 cut(s) 394, 424, 1103
BseRI GAGGAG 3 cut(s) 222, 450, 1052
BseX3I CGGCCG 1 cut(s) 241
BseXI GCAGC 1 cut(s) 815
BseYI CCCAGC 1 cut(s) 142
BsgI GTGCAG 1 cut(s) 995
Bsh1236I CGCG 1 cut(s) 453
Bsh1285I CGRYCG 2 cut(s) 244, 1111
BshFI GGCC 2 cut(s) 21, 243
BshNI GGYRCC 1 cut(s) 766
BsiEI CGRYCG 2 cut(s) 244, 1111
BsiHKAI GWGCWC 2 cut(s) 568, 649
BsiSI CCGG 1 cut(s) 413
BslFI GGGAC 1 cut(s) 598
BslI CCNNNNNNNGG 2 cut(s) 299, 419
BsmAI GTCTC 3 cut(s) 727, 1028, 1089
BsmFI GGGAC 1 cut(s) 598
BsnI GGCC 2 cut(s) 21, 243
Bso31I GGTCTC 1 cut(s) 727
Bsp1286I GDGCHC 3 cut(s) 140, 568, 649
Bsp143I GATC 3 cut(s) 84, 853, 1177
BspACI CCGC 3 cut(s) 104, 401, 451
BspANI GGCC 2 cut(s) 21, 243
BspCNI CTCAG 1 cut(s) 922
BspFNI CGCG 1 cut(s) 453
BspLI GGNNCC 4 cut(s) 118, 137, 586, 768
BspMAI CTGCAG 1 cut(s) 979
BspPI GGATC 3 cut(s) 92, 848, 1172
BspT107I GGYRCC 1 cut(s) 766
BspTI CTTAAG 1 cut(s) 946
BspTNI GGTCTC 1 cut(s) 727
BsrI ACTGG 3 cut(s) 394, 424, 1103
BssECI CCNNGG 1 cut(s) 238
BssMI GATC 3 cut(s) 84, 853, 1177
Bst2UI CCWGG 2 cut(s) 333, 843
Bst4CI ACNGT 3 cut(s) 294, 1016, 1136
Bst6I CTCTTC 1 cut(s) 575
BstAFI CTTAAG 1 cut(s) 946
BstAPI GCANNNNNTGC 1 cut(s) 500
BstC8I GCNNGC 1 cut(s) 881
BstDEI CTNAG 3 cut(s) 308, 909, 1192
BstF5I GGATG 2 cut(s) 136, 218
BstFNI CGCG 1 cut(s) 453
BstHHI GCGC 3 cut(s) 52, 455, 1191
BstKTI GATC 3 cut(s) 87, 856, 1180
BstMAI GTCTC 3 cut(s) 727, 1028, 1089
BstMBI GATC 3 cut(s) 84, 853, 1177
BstMCI CGRYCG 2 cut(s) 244, 1111
BstMWI GCNNNNNNNGC 3 cut(s) 116, 461, 500
BstNI CCWGG 2 cut(s) 333, 843
BstNSI RCATGY 1 cut(s) 656
BstSCI CCNGG 3 cut(s) 331, 411, 841
BstSFI CTRYAG 1 cut(s) 975
BstUI CGCG 1 cut(s) 453
BstV1I GCAGC 1 cut(s) 815
BstZI CGGCCG 1 cut(s) 241
BsuRI GGCC 2 cut(s) 21, 243
BtgZI GCGATG 1 cut(s) 387
BtsCI GGATG 2 cut(s) 136, 218
BtsIMutI CAGTG 2 cut(s) 387, 1012
Cac8I GCNNGC 1 cut(s) 881
CaiI CAGNNNCTG 2 cut(s) 369, 611
CfoI GCGC 3 cut(s) 52, 455, 1191
Cfr13I GGNCC 1 cut(s) 585
Csp6I GTAC 3 cut(s) 367, 767, 839
CviAII CATG 7 cut(s) 460, 653, 689, 816, 860, 922, 1163
CviQI GTAC 3 cut(s) 367, 767, 839
DdeI CTNAG 3 cut(s) 308, 909, 1192
DpnI GATC 3 cut(s) 86, 855, 1179
DpnII GATC 3 cut(s) 84, 853, 1177
EaeI YGGCCR 1 cut(s) 241
EagI CGGCCG 1 cut(s) 241
Eam1104I CTCTTC 1 cut(s) 575
EarI CTCTTC 1 cut(s) 575
EclXI CGGCCG 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 140
Eco31I GGTCTC 1 cut(s) 727
Eco47I GGWCC 1 cut(s) 585
Eco52I CGGCCG 1 cut(s) 241
Eco57I CTGAAG 4 cut(s) 80, 302, 415, 916
EcoRI GAATTC 1 cut(s) 303
EcoRII CCWGG 2 cut(s) 331, 841
EcoT38I GRGCYC 1 cut(s) 140
FaeI CATG 7 cut(s) 463, 656, 692, 819, 863, 925, 1166
FaqI GGGAC 1 cut(s) 598
FatI CATG 7 cut(s) 459, 652, 688, 815, 859, 921, 1162
FblI GTMKAC 1 cut(s) 408
Fnu4HI GCNGC 2 cut(s) 402, 804
FokI GGATG 2 cut(s) 143, 225
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 2 cut(s) 402, 804
FspBI CTAG 1 cut(s) 38
GlaI GCGC 3 cut(s) 51, 454, 1190
GluI GCNGC 2 cut(s) 402, 804
GsaI CCCAGC 1 cut(s) 146
GsuI CTGGAG 1 cut(s) 663
HaeIII GGCC 2 cut(s) 21, 243
HapII CCGG 1 cut(s) 413
HhaI GCGC 3 cut(s) 52, 455, 1191
Hin1II CATG 7 cut(s) 463, 656, 692, 819, 863, 925, 1166
Hin6I GCGC 3 cut(s) 50, 453, 1189
HinP1I GCGC 3 cut(s) 50, 453, 1189
HindIII AAGCTT 1 cut(s) 881
HinfI GANTC 5 cut(s) 31, 354, 775, 812, 998
HpaII CCGG 1 cut(s) 413
HphI GGTGA 3 cut(s) 17, 404, 894
Hpy166II GTNNAC 2 cut(s) 196, 409
Hpy188I TCNGA 4 cut(s) 99, 434, 607, 780
Hpy188III TCNNGA 4 cut(s) 174, 300, 642, 659
Hpy8I GTNNAC 2 cut(s) 196, 409
HpyAV CCTTC 3 cut(s) 104, 277, 588
HpyCH4III ACNGT 3 cut(s) 294, 1016, 1136
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 461, 500
HpyF3I CTNAG 3 cut(s) 308, 909, 1192
Hsp92II CATG 7 cut(s) 463, 656, 692, 819, 863, 925, 1166
HspAI GCGC 3 cut(s) 50, 453, 1189
KpnI GGTACC 1 cut(s) 770
Kzo9I GATC 3 cut(s) 84, 853, 1177
LmnI GCTCC 5 cut(s) 116, 135, 437, 644, 1171
Lsp1109I GCAGC 1 cut(s) 815
LweI GCATC 1 cut(s) 598
MaeI CTAG 1 cut(s) 38
MaeIII GTNAC 4 cut(s) 218, 226, 614, 679
MalI GATC 3 cut(s) 86, 855, 1179
MboI GATC 3 cut(s) 84, 853, 1177
MboII GAAGA 5 cut(s) 26, 536, 562, 712, 742
MfeI CAATTG 4 cut(s) 184, 343, 954, 1077
MhlI GDGCHC 3 cut(s) 140, 568, 649
MlyI GAGTC 1 cut(s) 992
MmeI TCCRAC 1 cut(s) 1072
MseI TTAA 4 cut(s) 180, 326, 624, 947
MspCI CTTAAG 1 cut(s) 946
MspI CCGG 1 cut(s) 413
MspR9I CCNGG 3 cut(s) 333, 413, 843
MunI CAATTG 4 cut(s) 184, 343, 954, 1077
MvaI CCWGG 2 cut(s) 333, 843
MvnI CGCG 1 cut(s) 453
MwoI GCNNNNNNNGC 3 cut(s) 116, 461, 500
NciI CCSGG 1 cut(s) 413
NdeII GATC 3 cut(s) 84, 853, 1177
NlaIII CATG 7 cut(s) 463, 656, 692, 819, 863, 925, 1166
NlaIV GGNNCC 4 cut(s) 118, 137, 586, 768
NmeAIII GCCGAG 1 cut(s) 219
NmuCI GTSAC 2 cut(s) 218, 226
NspI RCATGY 1 cut(s) 656
PciI ACATGT 1 cut(s) 652
PfeI GAWTC 4 cut(s) 31, 354, 775, 812
PflFI GACNNNGTC 1 cut(s) 1039
PkrI GCNGC 2 cut(s) 403, 805
PleI GAGTC 1 cut(s) 992
PpsI GAGTC 1 cut(s) 992
PscI ACATGT 1 cut(s) 652
PshBI ATTAAT 1 cut(s) 624
Psp6I CCWGG 2 cut(s) 331, 841
PspFI CCCAGC 1 cut(s) 142
PspGI CCWGG 2 cut(s) 331, 841
PspN4I GGNNCC 4 cut(s) 118, 137, 586, 768
PspPI GGNCC 1 cut(s) 585
PsrI GAACNNNNNNTAC 2 cut(s) 489, 521
PstI CTGCAG 1 cut(s) 979
PstNI CAGNNNCTG 2 cut(s) 369, 611
PsyI GACNNNGTC 1 cut(s) 1039
RsaI GTAC 3 cut(s) 368, 768, 840
RsaNI GTAC 3 cut(s) 367, 767, 839
SaqAI TTAA 4 cut(s) 180, 326, 624, 947
SatI GCNGC 2 cut(s) 402, 804
Sau3AI GATC 3 cut(s) 84, 853, 1177
Sau96I GGNCC 1 cut(s) 585
SchI GAGTC 1 cut(s) 992
ScrFI CCNGG 3 cut(s) 333, 413, 843
SduI GDGCHC 3 cut(s) 140, 568, 649
SetI ASST 8 cut(s) 148, 265, 368, 539, 580, 885, 915, 938
SfaNI GCATC 1 cut(s) 598
SfcI CTRYAG 1 cut(s) 975
SinI GGWCC 1 cut(s) 585
SmlI CTYRAG 1 cut(s) 946
SmoI CTYRAG 1 cut(s) 946
SsiI CCGC 3 cut(s) 104, 401, 451
SspI AATATT 1 cut(s) 789
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 3 cut(s) 331, 411, 841
TaaI ACNGT 3 cut(s) 294, 1016, 1136
TaqII GACCGA 1 cut(s) 1125
TauI GCSGC 1 cut(s) 404
TfiI GAWTC 4 cut(s) 31, 354, 775, 812
Tru1I TTAA 4 cut(s) 180, 326, 624, 947
Tru9I TTAA 4 cut(s) 180, 326, 624, 947
TscAI CASTG 2 cut(s) 394, 1019
TseFI GTSAC 2 cut(s) 218, 226
TseI GCWGC 1 cut(s) 803
Tsp45I GTSAC 2 cut(s) 218, 226
TspDTI ATGAA 5 cut(s) 255, 369, 536, 804, 982
TspRI CASTG 2 cut(s) 394, 1019
Tth111I GACNNNGTC 1 cut(s) 1039
Vha464I CTTAAG 1 cut(s) 946
VpaK11BI GGWCC 1 cut(s) 585
VspI ATTAAT 1 cut(s) 624
XapI RAATTY 2 cut(s) 303, 703
XceI RCATGY 1 cut(s) 656
XcmI CCANNNNNNNNNTGG 1 cut(s) 150
XmiI GTMKAC 1 cut(s) 408
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.