MD09G1232300.v1.1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000996
Physical Location & Seq
Forward (+)
28981461 .. 28984661
3201 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1232300.v1.1.491

Sequence Viewer

Length: 639 bp
ATGCTATTAATGGCCCGTAAAAGATTAGGCCAATGGTTAACTCTTCTAGTATGTTTACTGATATTGCTGAAAGCAGAAAGCCACCCTGTGGGAATCACTCTCGTCGAAAATGCAGTTGCTAAAGGAGCTGTGTGTTTGGATGGGAGCGCACCCGCTTACCACTTTGACAAGGGATTTGGTGCCGGAATCAACAATTGGTTGGTTCACGTGGAGGGTGGAGGATGGTGTAACAGCGTTGCAACTTGCCTTGATCGCAAGAATAATTACCGCGGTTCATCAACAAAAATGGAAAAGACAATGTCTTTTTCTGGGGTTCTTGGTAACAAGCAACAGACTAATCCAGACTTCTACAATTGGAATAGAATCAAAGTTAGGTACTGTGATGGCTCGTCATTCACTGGTGATGTTGAAGCAGTAGACCCAGCAACAAATCTTCACTTCAGAGGAGCAAGGGTTTTCCGTGCCATCATTGATGATTTATTGGCAAAAGGAATGAAAAACGCTCAAACTGCTCTTCTATCTGGTTGTTCGGCTGGCGGATTGGCCTCGATTCTTAATTGTGATAACTTTCATTCTCTCCTACCTGTGGGTACTAAAGTAAAATGTCTAGCAGATGCTGGTTACTTCATTAATGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

213

Amino Acids

22.88

Weight (kDa)

8.92

Isoelectric Point (pI)

24.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 24 - 211 2.2e-97 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 179
AccB7I CCANNNNNTGG 1 cut(s) 88
AccI GTMKAC 1 cut(s) 417
AccII CGCG 1 cut(s) 270
AciI CCGC 4 cut(s) 153, 268, 270, 537
AcuI CTGAAG 1 cut(s) 424
AcvI CACGTG 1 cut(s) 208
AdeI CACNNNGTG 1 cut(s) 88
AfaI GTAC 2 cut(s) 377, 592
AfiI CCNNNNNNNGG 2 cut(s) 88, 586
AgsI TTSAA 1 cut(s) 410
AjuI GAANNNNNNNTTGG 2 cut(s) 178, 210
AluBI AGCT 1 cut(s) 128
AluI AGCT 1 cut(s) 128
AlwNI CAGNNNCTG 1 cut(s) 617
AoxI GGCC 3 cut(s) 12, 28, 543
ArsI GACNNNNNNTTYG 2 cut(s) 158, 190
AseI ATTAAT 2 cut(s) 8, 630
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 1 cut(s) 13
AsuHPI GGTGA 1 cut(s) 413
BaeI ACNNNNGTAYC 2 cut(s) 582, 615
BanI GGYRCC 1 cut(s) 179
BbrPI CACGTG 1 cut(s) 208
BccI CCATC 4 cut(s) 134, 216, 377, 473
BfaI CTAG 2 cut(s) 47, 608
BmgT120I GGNCC 1 cut(s) 13
BmiI GGNNCC 1 cut(s) 181
BmsI GCATC 1 cut(s) 604
BsaAI YACGTR 1 cut(s) 208
BsaJI CCNNGG 1 cut(s) 268
BsaXI ACNNNNNCTCC 2 cut(s) 438, 468
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 586
Bse1I ACTGG 1 cut(s) 403
BseDI CCNNGG 1 cut(s) 268
BseGI GGATG 2 cut(s) 145, 227
BseLI CCNNNNNNNGG 2 cut(s) 88, 586
BseNI ACTGG 1 cut(s) 403
BseRI GAGGAG 1 cut(s) 459
BseYI CCCAGC 1 cut(s) 421
Bsh1236I CGCG 1 cut(s) 270
BshFI GGCC 3 cut(s) 14, 30, 545
BshNI GGYRCC 1 cut(s) 179
BsiSI CCGG 1 cut(s) 183
BslI CCNNNNNNNGG 2 cut(s) 88, 586
BsnI GGCC 3 cut(s) 14, 30, 545
Bsp143I GATC 1 cut(s) 250
BspACI CCGC 4 cut(s) 153, 268, 270, 537
BspANI GGCC 3 cut(s) 14, 30, 545
BspFNI CGCG 1 cut(s) 270
BspLI GGNNCC 1 cut(s) 181
BspQI GCTCTTC 1 cut(s) 519
BspT107I GGYRCC 1 cut(s) 179
BsrI ACTGG 1 cut(s) 403
BssECI CCNNGG 1 cut(s) 268
BssMI GATC 1 cut(s) 250
Bst4CI ACNGT 1 cut(s) 380
Bst6I CTCTTC 2 cut(s) 48, 519
BstBAI YACGTR 1 cut(s) 208
BstC8I GCNNGC 1 cut(s) 535
BstDSI CCRYGG 1 cut(s) 268
BstF5I GGATG 2 cut(s) 145, 227
BstFNI CGCG 1 cut(s) 270
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 1 cut(s) 253
BstMBI GATC 1 cut(s) 250
BstMWI GCNNNNNNNGC 3 cut(s) 125, 252, 509
BstUI CGCG 1 cut(s) 270
BsuRI GGCC 3 cut(s) 14, 30, 545
BtgI CCRYGG 1 cut(s) 268
BtsCI GGATG 2 cut(s) 145, 227
BtsIMutI CAGTG 1 cut(s) 396
Cac8I GCNNGC 1 cut(s) 535
CaiI CAGNNNCTG 1 cut(s) 617
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 13
Cfr42I CCGCGG 1 cut(s) 271
Csp6I GTAC 2 cut(s) 376, 591
CviJI RGCY 7 cut(s) 14, 30, 81, 128, 387, 533, 545
CviKI_1 RGCY 7 cut(s) 14, 30, 81, 128, 387, 533, 545
CviQI GTAC 2 cut(s) 376, 591
DpnI GATC 1 cut(s) 252
DpnII GATC 1 cut(s) 250
DraIII CACNNNGTG 1 cut(s) 88
Eam1104I CTCTTC 2 cut(s) 48, 519
EarI CTCTTC 2 cut(s) 48, 519
EciI GGCGGA 1 cut(s) 552
Eco57I CTGAAG 1 cut(s) 424
Eco72I CACGTG 1 cut(s) 208
FaiI YATR 1 cut(s) 52
FauI CCCGC 1 cut(s) 160
FblI GTMKAC 1 cut(s) 417
FokI GGATG 2 cut(s) 152, 234
FspBI CTAG 2 cut(s) 47, 608
GlaI GCGC 1 cut(s) 148
GsaI CCCAGC 1 cut(s) 425
HaeIII GGCC 3 cut(s) 14, 30, 545
HapII CCGG 1 cut(s) 183
HhaI GCGC 1 cut(s) 149
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HinfI GANTC 4 cut(s) 93, 186, 363, 550
HpaI GTTAAC 1 cut(s) 39
HpaII CCGG 1 cut(s) 183
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 4 cut(s) 39, 56, 205, 418
Hpy188I TCNGA 1 cut(s) 443
Hpy188III TCNNGA 1 cut(s) 341
Hpy8I GTNNAC 4 cut(s) 39, 56, 205, 418
Hpy99I CGWCG 1 cut(s) 107
HpyCH4III ACNGT 1 cut(s) 380
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 2 cut(s) 113, 239
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 252, 509
HpySE526I ACGT 1 cut(s) 207
HspAI GCGC 1 cut(s) 147
KspAI GTTAAC 1 cut(s) 39
KspI CCGCGG 1 cut(s) 271
Kzo9I GATC 1 cut(s) 250
LguI GCTCTTC 1 cut(s) 519
LmnI GCTCC 3 cut(s) 125, 144, 446
LweI GCATC 1 cut(s) 604
MaeI CTAG 2 cut(s) 47, 608
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 3 cut(s) 227, 320, 620
MalI GATC 1 cut(s) 252
MboI GATC 1 cut(s) 250
MboII GAAGA 3 cut(s) 35, 425, 506
MfeI CAATTG 2 cut(s) 193, 352
MluCI AATT 4 cut(s) 193, 262, 352, 556
MnlI CCTC 4 cut(s) 205, 212, 437, 556
MseI TTAA 4 cut(s) 8, 38, 555, 630
MspA1I CMGCKG 1 cut(s) 270
MspI CCGG 1 cut(s) 183
MunI CAATTG 2 cut(s) 193, 352
MvnI CGCG 1 cut(s) 270
MwoI GCNNNNNNNGC 3 cut(s) 125, 252, 509
NdeII GATC 1 cut(s) 250
NlaIV GGNNCC 1 cut(s) 181
PciSI GCTCTTC 1 cut(s) 519
PfeI GAWTC 4 cut(s) 93, 186, 363, 550
PflFI GACNNNGTC 1 cut(s) 298
PflMI CCANNNNNTGG 1 cut(s) 88
PmaCI CACGTG 1 cut(s) 208
PmlI CACGTG 1 cut(s) 208
Ppu21I YACGTR 1 cut(s) 208
PshBI ATTAAT 2 cut(s) 8, 630
PspCI CACGTG 1 cut(s) 208
PspFI CCCAGC 1 cut(s) 421
PspN4I GGNNCC 1 cut(s) 181
PspPI GGNCC 1 cut(s) 13
PstNI CAGNNNCTG 1 cut(s) 617
PsyI GACNNNGTC 1 cut(s) 298
RsaI GTAC 2 cut(s) 377, 592
RsaNI GTAC 2 cut(s) 376, 591
SacII CCGCGG 1 cut(s) 271
SapI GCTCTTC 1 cut(s) 519
SaqAI TTAA 4 cut(s) 8, 38, 555, 630
Sau3AI GATC 1 cut(s) 250
Sau96I GGNCC 1 cut(s) 13
SetI ASST 4 cut(s) 130, 210, 377, 586
SfaNI GCATC 1 cut(s) 604
Sfr303I CCGCGG 1 cut(s) 271
SgrBI CCGCGG 1 cut(s) 271
Sse9I AATT 4 cut(s) 193, 262, 352, 556
SsiI CCGC 4 cut(s) 153, 268, 270, 537
SspMI CTAG 2 cut(s) 47, 608
TaaI ACNGT 1 cut(s) 380
TaiI ACGT 1 cut(s) 210
TaqI TCGA 2 cut(s) 105, 548
TasI AATT 4 cut(s) 193, 262, 352, 556
TfiI GAWTC 4 cut(s) 93, 186, 363, 550
Tru1I TTAA 4 cut(s) 8, 38, 555, 630
Tru9I TTAA 4 cut(s) 8, 38, 555, 630
TscAI CASTG 1 cut(s) 403
TspDTI ATGAA 4 cut(s) 264, 509, 560, 616
TspGWI ACGGA 1 cut(s) 449
TspRI CASTG 1 cut(s) 403
Tth111I GACNNNGTC 1 cut(s) 298
Van91I CCANNNNNTGG 1 cut(s) 88
VspI ATTAAT 2 cut(s) 8, 630
XmiI GTMKAC 1 cut(s) 417
XspI CTAG 2 cut(s) 47, 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.