pycom08g17760

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000996
Physical Location & Seq
Reverse (-)
17898296 .. 17899872
1577 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g17760.1

Sequence Viewer

Length: 714 bp
ATGAAAAATGCTCAAAACGCTCTTCTATCTGGTTGTTCGGCTGGTGGATTAACCTCGATTCTTCATTGTGATAACTTTCGTTCTCTCCTACCTGTGGGTACTAAAGTAAAATGTCTAGCAGATGCTGGTTACTTCATTAATGCGAAGACTCTGTCTGGATCGCAACACATTCAAGATTTTTACAGTGAAGTGGTTGCGCTACATGGTTCGGCGAAGAACTTACCTGCATCCTGCACTTCAAGAATGAGTCCAGGGCTGTGCTTCTTCCCACAAAATGTGGTACCACAAACCCGAACGCCAATATTTTTGGTCAATGCAGCTTATGATTCGTGGCAGATAAAGAACATTTTGGCACCGGGTGTTGCTGATCCTAAGGGTGCCTGGAAAAAGTGCAAGCTTAATATAAAGAACTGCTCACCCAGTCAACTTCAAACCATGCAAGAATATAGGTTACAGTTCTTAAAAACAATTGCCGGTGCGAGCAATTCTGCATCTCATGGCGTGTTCATAGACTCTTGCTTTGCCCACTGCCAAATTGGAACGCAGGAGACATGGCTGGCAGCTGACTCTCCTATGTTGAGCAAGACGACAATTGCAAAGGCAGTTGGAGACTGGTATTACGACCGCACTGCGTTCAAAAAGATTGATTGCCCTTACCCTTGCAATCCCACTTGCAAAAACCGTGAATTTGACTCAAACGATCAGGAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

238

Amino Acids

25.93

Weight (kDa)

8.56

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 1 - 226 3.5e-102 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 232
Acc65I GGTACC 1 cut(s) 280
AccB1I GGYRCC 3 cut(s) 280, 352, 377
AciI CCGC 1 cut(s) 625
AclWI GGATC 2 cut(s) 166, 362
AcsI RAATTY 1 cut(s) 686
AdeI CACNNNGTG 1 cut(s) 359
AfaI GTAC 2 cut(s) 100, 282
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 4 cut(s) 173, 240, 431, 637
AjnI CCWGG 2 cut(s) 250, 380
AluBI AGCT 3 cut(s) 320, 397, 563
AluI AGCT 3 cut(s) 320, 397, 563
Alw26I GTCTC 2 cut(s) 542, 603
AlwI GGATC 2 cut(s) 166, 362
AlwNI CAGNNNCTG 1 cut(s) 125
ApeKI GCWGC 2 cut(s) 317, 560
ApoI RAATTY 1 cut(s) 686
ArsI GACNNNNNNTTYG 4 cut(s) 137, 169, 503, 535
AseI ATTAAT 1 cut(s) 138
Asp718I GGTACC 1 cut(s) 280
AspLEI GCGC 1 cut(s) 199
AsuC2I CCSGG 1 cut(s) 357
AsuHPI GGTGA 1 cut(s) 408
AxyI CCTNAGG 1 cut(s) 372
BaeI ACNNNNGTAYC 2 cut(s) 90, 123
BanI GGYRCC 3 cut(s) 280, 352, 377
BbsI GAAGAC 1 cut(s) 152
BbvI GCAGC 2 cut(s) 329, 572
BcgI CGANNNNNNTGC 2 cut(s) 611, 645
BciT130I CCWGG 2 cut(s) 252, 382
BcnI CCSGG 1 cut(s) 357
BcoDI GTCTC 2 cut(s) 542, 603
BfaI CTAG 1 cut(s) 116
BfuAI ACCTGC 1 cut(s) 232
BisI GCNGC 2 cut(s) 318, 561
BlsI GCNGC 2 cut(s) 319, 562
Bme1390I CCNGG 3 cut(s) 252, 357, 382
BmiI GGNNCC 3 cut(s) 282, 354, 379
BmrFI CCNGG 3 cut(s) 252, 357, 382
BmrI ACTGGG 1 cut(s) 414
BmsI GCATC 3 cut(s) 112, 236, 500
BmuI ACTGGG 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 152
BpuMI CCSGG 1 cut(s) 357
BsaJI CCNNGG 1 cut(s) 251
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse118I RCCGGY 1 cut(s) 473
Bse1I ACTGG 2 cut(s) 420, 617
Bse21I CCTNAGG 1 cut(s) 372
BseBI CCWGG 2 cut(s) 252, 382
BseDI CCNNGG 1 cut(s) 251
BseGI GGATG 1 cut(s) 227
BseLI CCNNNNNNNGG 1 cut(s) 94
BseNI ACTGG 2 cut(s) 420, 617
BseXI GCAGC 2 cut(s) 329, 572
BsgI GTGCAG 1 cut(s) 217
Bsh1285I CGRYCG 1 cut(s) 625
BshNI GGYRCC 3 cut(s) 280, 352, 377
BsiEI CGRYCG 1 cut(s) 625
BsiSI CCGG 2 cut(s) 356, 474
BslI CCNNNNNNNGG 1 cut(s) 94
BsmAI GTCTC 2 cut(s) 542, 603
Bsp143I GATC 3 cut(s) 158, 367, 700
BspACI CCGC 1 cut(s) 625
BspLI GGNNCC 3 cut(s) 282, 354, 379
BspMI ACCTGC 1 cut(s) 232
BspPI GGATC 2 cut(s) 166, 362
BspQI GCTCTTC 1 cut(s) 27
BspT107I GGYRCC 3 cut(s) 280, 352, 377
BsrFI RCCGGY 1 cut(s) 473
BsrI ACTGG 2 cut(s) 420, 617
BssAI RCCGGY 1 cut(s) 473
BssECI CCNNGG 1 cut(s) 251
BssMI GATC 3 cut(s) 158, 367, 700
Bst2UI CCWGG 2 cut(s) 252, 382
Bst4CI ACNGT 3 cut(s) 185, 456, 683
Bst6I CTCTTC 1 cut(s) 27
BstC8I GCNNGC 3 cut(s) 395, 481, 558
BstDEI CTNAG 1 cut(s) 372
BstF5I GGATG 1 cut(s) 227
BstHHI GCGC 1 cut(s) 199
BstKTI GATC 3 cut(s) 161, 370, 703
BstMAI GTCTC 2 cut(s) 542, 603
BstMBI GATC 3 cut(s) 158, 367, 700
BstMCI CGRYCG 1 cut(s) 625
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNI CCWGG 2 cut(s) 252, 382
BstSCI CCNGG 3 cut(s) 250, 355, 380
BstV1I GCAGC 2 cut(s) 329, 572
BstV2I GAAGAC 1 cut(s) 152
Bsu36I CCTNAGG 1 cut(s) 372
BtsCI GGATG 1 cut(s) 227
BtsI GCAGTG 2 cut(s) 526, 627
BtsIMutI CAGTG 3 cut(s) 190, 526, 627
BveI ACCTGC 1 cut(s) 232
Cac8I GCNNGC 3 cut(s) 395, 481, 558
CaiI CAGNNNCTG 1 cut(s) 125
CfoI GCGC 1 cut(s) 199
Cfr10I RCCGGY 1 cut(s) 473
Csp6I GTAC 2 cut(s) 99, 281
CviAII CATG 4 cut(s) 203, 436, 497, 552
CviJI RGCY 6 cut(s) 41, 256, 320, 397, 556, 563
CviKI_1 RGCY 6 cut(s) 41, 256, 320, 397, 556, 563
CviQI GTAC 2 cut(s) 99, 281
DdeI CTNAG 1 cut(s) 372
DpnI GATC 3 cut(s) 160, 369, 702
DpnII GATC 3 cut(s) 158, 367, 700
DraIII CACNNNGTG 1 cut(s) 359
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
Eco81I CCTNAGG 1 cut(s) 372
EcoRII CCWGG 2 cut(s) 250, 380
FaeI CATG 4 cut(s) 206, 439, 500, 555
FaiI YATR 9 cut(s) 204, 324, 404, 437, 447, 498, 509, 553, 575
FatI CATG 4 cut(s) 202, 435, 496, 551
Fnu4HI GCNGC 2 cut(s) 318, 561
FokI GGATG 1 cut(s) 214
Fsp4HI GCNGC 2 cut(s) 318, 561
FspBI CTAG 1 cut(s) 116
GlaI GCGC 1 cut(s) 198
GluI GCNGC 2 cut(s) 318, 561
HapII CCGG 2 cut(s) 356, 474
HhaI GCGC 1 cut(s) 199
Hin1II CATG 4 cut(s) 206, 439, 500, 555
Hin6I GCGC 1 cut(s) 197
HinP1I GCGC 1 cut(s) 197
HincII GTYRAC 1 cut(s) 425
HindII GTYRAC 1 cut(s) 425
HindIII AAGCTT 1 cut(s) 395
HinfI GANTC 7 cut(s) 58, 148, 247, 326, 512, 566, 692
HpaII CCGG 2 cut(s) 356, 474
HphI GGTGA 1 cut(s) 408
Hpy166II GTNNAC 1 cut(s) 425
Hpy188III TCNNGA 4 cut(s) 156, 173, 240, 704
Hpy8I GTNNAC 1 cut(s) 425
HpyCH4III ACNGT 3 cut(s) 185, 456, 683
HpyCH4V TGCA 9 cut(s) 227, 234, 317, 393, 439, 491, 596, 663, 675
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpyF3I CTNAG 1 cut(s) 372
Hsp92II CATG 4 cut(s) 206, 439, 500, 555
HspAI GCGC 1 cut(s) 197
KpnI GGTACC 1 cut(s) 284
Kzo9I GATC 3 cut(s) 158, 367, 700
LguI GCTCTTC 1 cut(s) 27
Lsp1109I GCAGC 2 cut(s) 329, 572
LweI GCATC 3 cut(s) 112, 236, 500
MaeI CTAG 1 cut(s) 116
MaeIII GTNAC 2 cut(s) 128, 450
MalI GATC 3 cut(s) 160, 369, 702
MboI GATC 3 cut(s) 158, 367, 700
MboII GAAGA 5 cut(s) 14, 53, 157, 226, 256
MfeI CAATTG 2 cut(s) 468, 591
MluCI AATT 5 cut(s) 468, 484, 534, 591, 686
MlyI GAGTC 5 cut(s) 142, 256, 506, 560, 686
MmeI TCCRAC 1 cut(s) 586
MnlI CCTC 1 cut(s) 64
MseI TTAA 5 cut(s) 50, 138, 399, 461, 712
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 2 cut(s) 356, 474
MspR9I CCNGG 3 cut(s) 252, 357, 382
MunI CAATTG 2 cut(s) 468, 591
MvaI CCWGG 2 cut(s) 252, 382
MwoI GCNNNNNNNGC 1 cut(s) 17
NciI CCSGG 1 cut(s) 357
NdeII GATC 3 cut(s) 158, 367, 700
NlaIII CATG 4 cut(s) 206, 439, 500, 555
NlaIV GGNNCC 3 cut(s) 282, 354, 379
PciSI GCTCTTC 1 cut(s) 27
PfeI GAWTC 2 cut(s) 58, 326
PflFI GACNNNGTC 1 cut(s) 151
PkrI GCNGC 2 cut(s) 319, 562
PleI GAGTC 5 cut(s) 142, 255, 506, 560, 686
PpsI GAGTC 5 cut(s) 142, 255, 506, 560, 686
PshBI ATTAAT 1 cut(s) 138
Psp6I CCWGG 2 cut(s) 250, 380
PspGI CCWGG 2 cut(s) 250, 380
PspN4I GGNNCC 3 cut(s) 282, 354, 379
PstNI CAGNNNCTG 1 cut(s) 125
PsyI GACNNNGTC 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 2 cut(s) 100, 282
RsaNI GTAC 2 cut(s) 99, 281
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 5 cut(s) 50, 138, 399, 461, 712
SatI GCNGC 2 cut(s) 318, 561
Sau3AI GATC 3 cut(s) 158, 367, 700
SchI GAGTC 5 cut(s) 142, 256, 506, 560, 686
ScrFI CCNGG 3 cut(s) 252, 357, 382
SetI ASST 7 cut(s) 56, 94, 226, 322, 399, 452, 565
SfaNI GCATC 3 cut(s) 112, 236, 500
Sse9I AATT 5 cut(s) 468, 484, 534, 591, 686
SsiI CCGC 1 cut(s) 625
SspI AATATT 1 cut(s) 303
SspMI CTAG 1 cut(s) 116
StyD4I CCNGG 3 cut(s) 250, 355, 380
TaaI ACNGT 3 cut(s) 185, 456, 683
TaqI TCGA 1 cut(s) 56
TasI AATT 5 cut(s) 468, 484, 534, 591, 686
TfiI GAWTC 2 cut(s) 58, 326
Tru1I TTAA 5 cut(s) 50, 138, 399, 461, 712
Tru9I TTAA 5 cut(s) 50, 138, 399, 461, 712
TscAI CASTG 3 cut(s) 190, 533, 634
TseI GCWGC 2 cut(s) 317, 560
TspDTI ATGAA 4 cut(s) 17, 53, 124, 496
TspRI CASTG 3 cut(s) 190, 533, 634
Tth111I GACNNNGTC 1 cut(s) 151
VspI ATTAAT 1 cut(s) 138
XapI RAATTY 1 cut(s) 686
XcmI CCANNNNNNNNNTGG 1 cut(s) 533
XspI CTAG 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.