RLG00000002704

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000996
Physical Location & Seq
Forward (+)
38363462 .. 38367191
3730 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002704

Sequence Viewer

Length: 1242 bp
ATGGAAGCCAGTGGTTGGATGCATTTAGATTTTCTCTCATACTACACTTTCAGAATGGCCTATGCAAGATTAGGCCAGTGGTTAGGTCTTCTGGTATGTTTACTGATATTGCTTAAAGCTGAAAGCCATCCTGTGGAACTCACTTTGGTCCAGAGTGCAGTTGCCAAAGGAGCTGTTTGTTTAGACGGGAGCCCACCGGCATACCACTTTGACAAGGGATTTGGTGCAGGGATTAACAATTGGTTGGTTCATATTGAGGGAGGAGGATGGTGTAAGGATGTTGAGAGTTGCGTTGAGCGAACGAAAAACTTCAGAGGTTCATCCACAAAAATGGAAAAGACAATATCTTTTTCTGGGGTTCTTGGTAAAAAGCAAACTATGAATCCAGACTTCTATAACTGGAACAGAATCAAGGTTAGGTACTGTGATGGGTCATCATTCACCGGTGATGTGGAAGCAGTAGACCCAGCTACAAAGCTTCACTTCAGAGGAGGAAGGGTTTTTCGTGCCATCATTGATGATCTATTAGCAAAGGGAATGATAAATGCCAAAAATGCTCTACTCTCTGGTTGTTCGGCTGGAGGATTGGCTTCTATTCTTCAATGTGATAACTTCCATTCTCTCTTACCCGCCGGAACTAATGTGAAATGCCTTGCGGATGCTGGTTACTTCATCAATGCGAAGACTATTACTGGGTCACAACAAATTGAAGCCTTTTATAGTCAAGTGGTTGCACTACATGGTTCAGCAAAGCATTTACCTGCATCCTGCACTTCAAGATTGACACCTGGGTTGTGCTTCTTCCCACAAAATGTGATACCGCAACTCCGGACTCCTATTTTTTTGGTAAATGCTGCTTATGATTCGTGGCAGATAAAGAACATTTTGGCACCGAGTATTGCTGATCCTAAAGGCACATGGAAAAGCTGCAAGTCTGGTATAAAGAACTGCTCACCTGCGCAAATTCAAACCATGCAAGATTATAGGTTACAGTTCTTAGATGTACTCAATGGTGGTCTCACCAACTCTCCATCTCATGGAGCATTCATAGACTCTTGCTATGCTCACTGCCAAATTGGAACTCAAGAGACATGGATGGCAGCTGACTCCCCGGTTGTCAGTAAGACGACAATTGCAAAGGCCGTCGGAGACTGGTATTACAACCGAAGTCCATCAAAAAAGATCGATTGTCCTTACCCTTGCAATCCTACCTGCAAAAATAAAGAATTTGATTCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

414

Amino Acids

45.21

Weight (kDa)

8.65

Isoelectric Point (pI)

45.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 39 - 406 4.3e-175 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 964
Acc16I TGCGCA 1 cut(s) 960
Acc36I ACCTGC 3 cut(s) 769, 964, 1220
AccB1I GGYRCC 1 cut(s) 889
AccB7I CCANNNNNTGG 3 cut(s) 15, 133, 1037
AccI GTMKAC 1 cut(s) 462
AccIII TCCGGA 1 cut(s) 828
AciI CCGC 3 cut(s) 630, 656, 821
AclWI GGATC 1 cut(s) 899
AcsI RAATTY 2 cut(s) 963, 1226
AcuI CTGAAG 2 cut(s) 295, 469
AfaI GTAC 2 cut(s) 422, 1005
AfiI CCNNNNNNNGG 3 cut(s) 15, 133, 1037
AgeI ACCGGT 1 cut(s) 443
AgsI TTSAA 5 cut(s) 602, 710, 777, 968, 1236
AjnI CCWGG 1 cut(s) 787
AluBI AGCT 6 cut(s) 119, 173, 470, 478, 927, 1103
AluI AGCT 6 cut(s) 119, 173, 470, 478, 927, 1103
Alw26I GTCTC 3 cut(s) 1022, 1082, 1143
AlwI GGATC 1 cut(s) 899
Aor13HI TCCGGA 1 cut(s) 828
AoxI GGCC 3 cut(s) 57, 73, 1140
ApeKI GCWGC 3 cut(s) 854, 927, 1100
ApoI RAATTY 2 cut(s) 963, 1226
ArsI GACNNNNNNTTYG 2 cut(s) 203, 235
AsiGI ACCGGT 1 cut(s) 443
Asp700I GAANNNNTTC 1 cut(s) 308
AspLEI GCGC 1 cut(s) 961
AspS9I GGNCC 1 cut(s) 148
AsuC2I CCSGG 1 cut(s) 1112
AsuHPI GGTGA 4 cut(s) 433, 458, 945, 1012
AvaII GGWCC 1 cut(s) 148
BanI GGYRCC 1 cut(s) 889
BanII GRGCYC 1 cut(s) 194
BbsI GAAGAC 2 cut(s) 80, 689
BbvI GCAGC 3 cut(s) 841, 914, 1112
BccI CCATC 7 cut(s) 135, 261, 422, 518, 1039, 1090, 1180
BceAI ACGGC 1 cut(s) 1127
BciT130I CCWGG 1 cut(s) 789
BcnI CCSGG 1 cut(s) 1112
BcoDI GTCTC 3 cut(s) 1022, 1082, 1143
BfaI CTAG 1 cut(s) 1240
BfuAI ACCTGC 3 cut(s) 769, 964, 1220
BisI GCNGC 3 cut(s) 855, 928, 1101
BlsI GCNGC 3 cut(s) 856, 929, 1102
Bme1390I CCNGG 2 cut(s) 789, 1112
Bme18I GGWCC 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 148
BmiI GGNNCC 2 cut(s) 191, 891
BmrFI CCNGG 2 cut(s) 789, 1112
BmrI ACTGGG 1 cut(s) 702
BmsI GCATC 3 cut(s) 9, 649, 773
BmuI ACTGGG 1 cut(s) 702
BpiI GAAGAC 2 cut(s) 80, 689
BpmI CTGGAG 1 cut(s) 600
BpuEI CTTGAG 1 cut(s) 1068
BpuMI CCSGG 1 cut(s) 1112
Bsa29I ATCGAT 1 cut(s) 1185
BsaI GGTCTC 1 cut(s) 1022
BsaJI CCNNGG 2 cut(s) 788, 1110
BsaWI WCCGGW 2 cut(s) 443, 828
BsaXI ACNNNNNCTCC 6 cut(s) 483, 513, 810, 840, 1012, 1042
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 133, 1037
Bse118I RCCGGY 2 cut(s) 196, 443
Bse1I ACTGG 5 cut(s) 9, 76, 404, 697, 1157
BseAI TCCGGA 1 cut(s) 828
BseBI CCWGG 1 cut(s) 789
BseCI ATCGAT 1 cut(s) 1185
BseDI CCNNGG 2 cut(s) 788, 1110
BseGI GGATG 8 cut(s) 24, 127, 272, 283, 320, 664, 764, 1101
BseLI CCNNNNNNNGG 3 cut(s) 15, 133, 1037
BseNI ACTGG 5 cut(s) 9, 76, 404, 697, 1157
BseRI GAGGAG 2 cut(s) 276, 504
BseXI GCAGC 3 cut(s) 841, 914, 1112
BseYI CCCAGC 1 cut(s) 466
BsgI GTGCAG 3 cut(s) 177, 246, 754
BshFI GGCC 3 cut(s) 59, 75, 1142
BshNI GGYRCC 1 cut(s) 889
BshTI ACCGGT 1 cut(s) 443
BshVI ATCGAT 1 cut(s) 1185
BsiSI CCGG 5 cut(s) 197, 444, 633, 829, 1112
BslI CCNNNNNNNGG 3 cut(s) 15, 133, 1037
BsmAI GTCTC 3 cut(s) 1022, 1082, 1143
BsmI GAATGC 1 cut(s) 1043
BsnI GGCC 3 cut(s) 59, 75, 1142
Bso31I GGTCTC 1 cut(s) 1022
Bsp1286I GDGCHC 1 cut(s) 194
Bsp13I TCCGGA 1 cut(s) 828
Bsp143I GATC 3 cut(s) 520, 904, 1182
BspACI CCGC 3 cut(s) 630, 656, 821
BspANI GGCC 3 cut(s) 59, 75, 1142
BspDI ATCGAT 1 cut(s) 1185
BspEI TCCGGA 1 cut(s) 828
BspLI GGNNCC 2 cut(s) 191, 891
BspMI ACCTGC 3 cut(s) 769, 964, 1220
BspPI GGATC 1 cut(s) 899
BspT107I GGYRCC 1 cut(s) 889
BspTNI GGTCTC 1 cut(s) 1022
BsrFI RCCGGY 2 cut(s) 196, 443
BsrI ACTGG 5 cut(s) 9, 76, 404, 697, 1157
BssAI RCCGGY 2 cut(s) 196, 443
BssECI CCNNGG 2 cut(s) 788, 1110
BssMI GATC 3 cut(s) 520, 904, 1182
Bst2UI CCWGG 1 cut(s) 789
Bst4CI ACNGT 2 cut(s) 425, 993
BstDEI CTNAG 1 cut(s) 997
BstF5I GGATG 8 cut(s) 24, 127, 272, 283, 320, 664, 764, 1101
BstHHI GCGC 1 cut(s) 961
BstKTI GATC 3 cut(s) 523, 907, 1185
BstMAI GTCTC 3 cut(s) 1022, 1082, 1143
BstMBI GATC 3 cut(s) 520, 904, 1182
BstMWI GCNNNNNNNGC 2 cut(s) 170, 554
BstNI CCWGG 1 cut(s) 789
BstSCI CCNGG 2 cut(s) 787, 1110
BstV1I GCAGC 3 cut(s) 841, 914, 1112
BstV2I GAAGAC 2 cut(s) 80, 689
BstXI CCANNNNNNTGG 1 cut(s) 331
Bsu15I ATCGAT 1 cut(s) 1185
BsuRI GGCC 3 cut(s) 59, 75, 1142
BsuTUI ATCGAT 1 cut(s) 1185
BtsCI GGATG 8 cut(s) 24, 127, 272, 283, 320, 664, 764, 1101
BtsI GCAGTG 1 cut(s) 1066
BtsIMutI CAGTG 3 cut(s) 16, 83, 1066
BveI ACCTGC 3 cut(s) 769, 964, 1220
CfoI GCGC 1 cut(s) 961
Cfr10I RCCGGY 2 cut(s) 196, 443
Cfr13I GGNCC 1 cut(s) 148
ClaI ATCGAT 1 cut(s) 1185
Csp6I GTAC 2 cut(s) 421, 1004
CspAI ACCGGT 1 cut(s) 443
CviAII CATG 5 cut(s) 740, 918, 973, 1037, 1092
CviQI GTAC 2 cut(s) 421, 1004
DdeI CTNAG 1 cut(s) 997
DpnI GATC 3 cut(s) 522, 906, 1184
DpnII GATC 3 cut(s) 520, 904, 1182
Eco24I GRGCYC 1 cut(s) 194
Eco31I GGTCTC 1 cut(s) 1022
Eco47I GGWCC 1 cut(s) 148
Eco57I CTGAAG 2 cut(s) 295, 469
EcoRII CCWGG 1 cut(s) 787
EcoT22I ATGCAT 1 cut(s) 24
EcoT38I GRGCYC 1 cut(s) 194
FaeI CATG 5 cut(s) 743, 921, 976, 1040, 1095
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FatI CATG 5 cut(s) 739, 917, 972, 1036, 1091
FauI CCCGC 1 cut(s) 637
FblI GTMKAC 1 cut(s) 462
Fnu4HI GCNGC 3 cut(s) 855, 928, 1101
FokI GGATG 8 cut(s) 31, 114, 279, 290, 307, 671, 751, 1108
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 3 cut(s) 855, 928, 1101
FspBI CTAG 1 cut(s) 1240
FspI TGCGCA 1 cut(s) 960
GlaI GCGC 1 cut(s) 960
GluI GCNGC 3 cut(s) 855, 928, 1101
GsaI CCCAGC 1 cut(s) 470
GsuI CTGGAG 1 cut(s) 600
HaeIII GGCC 3 cut(s) 59, 75, 1142
HapII CCGG 5 cut(s) 197, 444, 633, 829, 1112
HhaI GCGC 1 cut(s) 961
Hin1II CATG 5 cut(s) 743, 921, 976, 1040, 1095
Hin6I GCGC 1 cut(s) 959
HinP1I GCGC 1 cut(s) 959
HindIII AAGCTT 1 cut(s) 476
HinfI GANTC 7 cut(s) 382, 408, 832, 863, 1052, 1106, 1232
HpaII CCGG 5 cut(s) 197, 444, 633, 829, 1112
HphI GGTGA 4 cut(s) 433, 458, 945, 1012
Hpy166II GTNNAC 2 cut(s) 101, 463
Hpy188I TCNGA 4 cut(s) 53, 314, 488, 1148
Hpy188III TCNNGA 5 cut(s) 151, 386, 777, 829, 1085
Hpy8I GTNNAC 2 cut(s) 101, 463
Hpy99I CGWCG 1 cut(s) 1148
HpyAV CCTTC 1 cut(s) 489
HpyCH4III ACNGT 2 cut(s) 425, 993
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 554
HpyF3I CTNAG 1 cut(s) 997
Hsp92II CATG 5 cut(s) 743, 921, 976, 1040, 1095
HspAI GCGC 1 cut(s) 959
Kpn2I TCCGGA 1 cut(s) 828
Kzo9I GATC 3 cut(s) 520, 904, 1182
LmnI GCTCC 3 cut(s) 170, 189, 1040
Lsp1109I GCAGC 3 cut(s) 841, 914, 1112
LweI GCATC 3 cut(s) 9, 649, 773
MaeI CTAG 1 cut(s) 1240
MaeIII GTNAC 3 cut(s) 665, 696, 987
MalI GATC 3 cut(s) 522, 906, 1184
MboI GATC 3 cut(s) 520, 904, 1182
MboII GAAGA 4 cut(s) 80, 590, 694, 793
MfeI CAATTG 2 cut(s) 238, 1131
MhlI GDGCHC 1 cut(s) 194
MluCI AATT 6 cut(s) 238, 705, 963, 1074, 1131, 1226
MlyI GAGTC 3 cut(s) 826, 1046, 1100
MmeI TCCRAC 1 cut(s) 1126
MnlI CCTC 7 cut(s) 250, 254, 257, 308, 482, 485, 575
Mph1103I ATGCAT 1 cut(s) 24
MroI TCCGGA 1 cut(s) 828
MroXI GAANNNNTTC 1 cut(s) 308
MseI TTAA 2 cut(s) 114, 234
MslI CAYNNNNRTG 1 cut(s) 329
MspA1I CMGCKG 1 cut(s) 1103
MspI CCGG 5 cut(s) 197, 444, 633, 829, 1112
MspR9I CCNGG 2 cut(s) 789, 1112
MunI CAATTG 2 cut(s) 238, 1131
Mva1269I GAATGC 1 cut(s) 1043
MvaI CCWGG 1 cut(s) 789
MwoI GCNNNNNNNGC 2 cut(s) 170, 554
NciI CCSGG 1 cut(s) 1112
NdeII GATC 3 cut(s) 520, 904, 1182
NlaIII CATG 5 cut(s) 743, 921, 976, 1040, 1095
NlaIV GGNNCC 2 cut(s) 191, 891
NmuCI GTSAC 1 cut(s) 696
NsbI TGCGCA 1 cut(s) 960
NsiI ATGCAT 1 cut(s) 24
PaqCI CACCTGC 1 cut(s) 964
PctI GAATGC 1 cut(s) 1043
PdmI GAANNNNTTC 1 cut(s) 308
PfeI GAWTC 4 cut(s) 382, 408, 863, 1232
PflMI CCANNNNNTGG 3 cut(s) 15, 133, 1037
PinAI ACCGGT 1 cut(s) 443
PkrI GCNGC 3 cut(s) 856, 929, 1102
PleI GAGTC 3 cut(s) 826, 1046, 1100
PpsI GAGTC 3 cut(s) 826, 1046, 1100
Psp6I CCWGG 1 cut(s) 787
PspFI CCCAGC 1 cut(s) 466
PspGI CCWGG 1 cut(s) 787
PspN4I GGNNCC 2 cut(s) 191, 891
PspPI GGNCC 1 cut(s) 148
PvuII CAGCTG 1 cut(s) 1103
RsaI GTAC 2 cut(s) 422, 1005
RsaNI GTAC 2 cut(s) 421, 1004
RseI CAYNNNNRTG 1 cut(s) 329
SaqAI TTAA 2 cut(s) 114, 234
SatI GCNGC 3 cut(s) 855, 928, 1101
Sau3AI GATC 3 cut(s) 520, 904, 1182
Sau96I GGNCC 1 cut(s) 148
SchI GAGTC 3 cut(s) 826, 1046, 1100
ScrFI CCNGG 2 cut(s) 789, 1112
SduI GDGCHC 1 cut(s) 194
SfaNI GCATC 3 cut(s) 9, 649, 773
SgrAI CRCCGGYG 1 cut(s) 443
SinI GGWCC 1 cut(s) 148
SmiMI CAYNNNNRTG 1 cut(s) 329
SmlI CTYRAG 1 cut(s) 1083
SmoI CTYRAG 1 cut(s) 1083
Sse9I AATT 6 cut(s) 238, 705, 963, 1074, 1131, 1226
SsiI CCGC 3 cut(s) 630, 656, 821
SspMI CTAG 1 cut(s) 1240
StyD4I CCNGG 2 cut(s) 787, 1110
TaaI ACNGT 2 cut(s) 425, 993
TaqI TCGA 1 cut(s) 1185
TasI AATT 6 cut(s) 238, 705, 963, 1074, 1131, 1226
TatI WGTACW 1 cut(s) 1003
TfiI GAWTC 4 cut(s) 382, 408, 863, 1232
Tru1I TTAA 2 cut(s) 114, 234
Tru9I TTAA 2 cut(s) 114, 234
TscAI CASTG 3 cut(s) 16, 83, 1073
TseFI GTSAC 1 cut(s) 696
TseI GCWGC 3 cut(s) 854, 927, 1100
Tsp45I GTSAC 1 cut(s) 696
TspDTI ATGAA 5 cut(s) 239, 309, 395, 661, 1036
TspRI CASTG 3 cut(s) 16, 83, 1073
Van91I CCANNNNNTGG 3 cut(s) 15, 133, 1037
VpaK11BI GGWCC 1 cut(s) 148
XapI RAATTY 2 cut(s) 963, 1226
XmiI GTMKAC 1 cut(s) 462
XmnI GAANNNNTTC 1 cut(s) 308
XspI CTAG 1 cut(s) 1240
Zsp2I ATGCAT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.