Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 1076 |
| AccI | GTMKAC | 1 cut(s) | 1038 |
| AccII | CGCG | 1 cut(s) | 24 |
| AccIII | TCCGGA | 1 cut(s) | 1150 |
| AciI | CCGC | 7 cut(s) | 340, 797, 800, 997, 1076, 1363, 1470 |
| AcsI | RAATTY | 2 cut(s) | 207, 1190 |
| AcuI | CTGAAG | 1 cut(s) | 146 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 129, 889, 1016, 1068, 1069, 1136, 1157 |
| AgsI | TTSAA | 4 cut(s) | 779, 958, 1027, 1250 |
| AjnI | CCWGG | 2 cut(s) | 128, 1365 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 413, 445 |
| AluBI | AGCT | 7 cut(s) | 788, 819, 1124, 1172, 1258, 1397, 1458 |
| AluI | AGCT | 7 cut(s) | 788, 819, 1124, 1172, 1258, 1397, 1458 |
| Alw21I | GWGCWC | 2 cut(s) | 508, 1158 |
| Alw26I | GTCTC | 8 cut(s) | 91, 302, 647, 727, 813, 819, 1152, 1223 |
| Aor13HI | TCCGGA | 1 cut(s) | 1150 |
| AoxI | GGCC | 3 cut(s) | 327, 763, 1360 |
| ApeKI | GCWGC | 5 cut(s) | 788, 1394, 1414, 1458, 1467 |
| ApoI | RAATTY | 2 cut(s) | 207, 1190 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1031, 1063 |
| AseI | ATTAAT | 2 cut(s) | 375, 1032 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 211 |
| AspLEI | GCGC | 1 cut(s) | 24 |
| AspS9I | GGNCC | 3 cut(s) | 749, 880, 1201 |
| AsuHPI | GGTGA | 4 cut(s) | 108, 922, 1327, 1360 |
| AsuNHI | GCTAGC | 1 cut(s) | 1168 |
| AvaII | GGWCC | 3 cut(s) | 749, 880, 1201 |
| Bbv12I | GWGCWC | 2 cut(s) | 508, 1158 |
| BbvI | GCAGC | 5 cut(s) | 775, 1406, 1426, 1445, 1479 |
| BccI | CCATC | 5 cut(s) | 185, 346, 416, 494, 1392 |
| BceAI | ACGGC | 1 cut(s) | 1446 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 601, 635 |
| BciT130I | CCWGG | 2 cut(s) | 130, 1367 |
| BcoDI | GTCTC | 8 cut(s) | 91, 302, 647, 727, 813, 819, 1152, 1223 |
| BfaI | CTAG | 3 cut(s) | 47, 927, 1169 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 14, 1368 |
| BisI | GCNGC | 8 cut(s) | 341, 789, 798, 1363, 1395, 1415, 1459, 1468 |
| BlsI | GCNGC | 8 cut(s) | 342, 790, 799, 1364, 1396, 1416, 1460, 1469 |
| Bme1390I | CCNGG | 2 cut(s) | 130, 1367 |
| Bme18I | GGWCC | 3 cut(s) | 749, 880, 1201 |
| BmgT120I | GGNCC | 3 cut(s) | 749, 880, 1201 |
| BmiI | GGNNCC | 1 cut(s) | 881 |
| BmrFI | CCNGG | 2 cut(s) | 130, 1367 |
| BmrI | ACTGGG | 1 cut(s) | 1146 |
| BmsI | GCATC | 2 cut(s) | 886, 1225 |
| BmtI | GCTAGC | 1 cut(s) | 1172 |
| BmuI | ACTGGG | 1 cut(s) | 1146 |
| BpmI | CTGGAG | 1 cut(s) | 151 |
| BsaJI | CCNNGG | 2 cut(s) | 417, 1366 |
| BsaWI | WCCGGW | 2 cut(s) | 1012, 1150 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 559, 589 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 129, 889, 1016, 1068, 1069, 1136, 1157 |
| Bse1I | ACTGG | 2 cut(s) | 1141, 1279 |
| Bse3DI | GCAATG | 2 cut(s) | 1191, 1378 |
| BseAI | TCCGGA | 1 cut(s) | 1150 |
| BseBI | CCWGG | 2 cut(s) | 130, 1367 |
| BseDI | CCNNGG | 2 cut(s) | 417, 1366 |
| BseGI | GGATG | 5 cut(s) | 196, 328, 427, 486, 901 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 129, 889, 1016, 1068, 1069, 1136, 1157 |
| BseMI | GCAATG | 2 cut(s) | 1191, 1378 |
| BseMII | CTCAG | 2 cut(s) | 716, 1250 |
| BseNI | ACTGG | 2 cut(s) | 1141, 1279 |
| BseRI | GAGGAG | 3 cut(s) | 96, 1134, 1432 |
| BseXI | GCAGC | 5 cut(s) | 775, 1406, 1426, 1445, 1479 |
| BsgI | GTGCAG | 1 cut(s) | 641 |
| Bsh1236I | CGCG | 1 cut(s) | 24 |
| BshFI | GGCC | 3 cut(s) | 329, 765, 1362 |
| BsiHKAI | GWGCWC | 2 cut(s) | 508, 1158 |
| BsiSI | CCGG | 3 cut(s) | 230, 1013, 1151 |
| BslFI | GGGAC | 2 cut(s) | 893, 1308 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 129, 889, 1016, 1068, 1069, 1136, 1157 |
| BsmAI | GTCTC | 8 cut(s) | 91, 302, 647, 727, 813, 819, 1152, 1223 |
| BsmFI | GGGAC | 2 cut(s) | 893, 1308 |
| BsmI | GAATGC | 1 cut(s) | 1046 |
| BsnI | GGCC | 3 cut(s) | 329, 765, 1362 |
| Bsp1286I | GDGCHC | 2 cut(s) | 508, 1158 |
| Bsp13I | TCCGGA | 1 cut(s) | 1150 |
| Bsp143I | GATC | 2 cut(s) | 868, 1051 |
| BspACI | CCGC | 7 cut(s) | 340, 797, 800, 997, 1076, 1363, 1470 |
| BspANI | GGCC | 3 cut(s) | 329, 765, 1362 |
| BspCNI | CTCAG | 2 cut(s) | 715, 1251 |
| BspEI | TCCGGA | 1 cut(s) | 1150 |
| BspFNI | CGCG | 1 cut(s) | 24 |
| BspLI | GGNNCC | 1 cut(s) | 881 |
| BspOI | GCTAGC | 1 cut(s) | 1172 |
| BsrBI | CCGCTC | 1 cut(s) | 1076 |
| BsrDI | GCAATG | 2 cut(s) | 1191, 1378 |
| BsrI | ACTGG | 2 cut(s) | 1141, 1279 |
| BssECI | CCNNGG | 2 cut(s) | 417, 1366 |
| BssMI | GATC | 2 cut(s) | 868, 1051 |
| BssT1I | CCWWGG | 1 cut(s) | 417 |
| Bst2UI | CCWGG | 2 cut(s) | 130, 1367 |
| Bst4CI | ACNGT | 1 cut(s) | 252 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1400 |
| BstC8I | GCNNGC | 5 cut(s) | 26, 1078, 1113, 1170, 1405 |
| BstDEI | CTNAG | 2 cut(s) | 702, 1259 |
| BstF5I | GGATG | 5 cut(s) | 196, 328, 427, 486, 901 |
| BstFNI | CGCG | 1 cut(s) | 24 |
| BstHHI | GCGC | 1 cut(s) | 24 |
| BstKTI | GATC | 2 cut(s) | 871, 1054 |
| BstMAI | GTCTC | 8 cut(s) | 91, 302, 647, 727, 813, 819, 1152, 1223 |
| BstMBI | GATC | 2 cut(s) | 868, 1051 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 14, 794, 797, 1121, 1368, 1400, 1464, 1467 |
| BstNI | CCWGG | 2 cut(s) | 130, 1367 |
| BstNSI | RCATGY | 1 cut(s) | 795 |
| BstSCI | CCNGG | 2 cut(s) | 128, 1365 |
| BstUI | CGCG | 1 cut(s) | 24 |
| BstV1I | GCAGC | 5 cut(s) | 775, 1406, 1426, 1445, 1479 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 265 |
| BsuRI | GGCC | 3 cut(s) | 329, 765, 1362 |
| BtsCI | GGATG | 5 cut(s) | 196, 328, 427, 486, 901 |
| BtsI | GCAGTG | 1 cut(s) | 369 |
| BtsIMutI | CAGTG | 2 cut(s) | 369, 1134 |
| Cac8I | GCNNGC | 5 cut(s) | 26, 1078, 1113, 1170, 1405 |
| CfoI | GCGC | 1 cut(s) | 24 |
| Cfr13I | GGNCC | 3 cut(s) | 749, 880, 1201 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 888, 923 |
| CviAII | CATG | 5 cut(s) | 110, 758, 792, 1055, 1355 |
| DdeI | CTNAG | 2 cut(s) | 702, 1259 |
| DpnI | GATC | 2 cut(s) | 870, 1053 |
| DpnII | GATC | 2 cut(s) | 868, 1051 |
| EciI | GGCGGA | 1 cut(s) | 815 |
| Eco130I | CCWWGG | 1 cut(s) | 417 |
| Eco47I | GGWCC | 3 cut(s) | 749, 880, 1201 |
| Eco57I | CTGAAG | 1 cut(s) | 146 |
| EcoRII | CCWGG | 2 cut(s) | 128, 1365 |
| EcoT14I | CCWWGG | 1 cut(s) | 417 |
| ErhI | CCWWGG | 1 cut(s) | 417 |
| FaeI | CATG | 5 cut(s) | 113, 761, 795, 1058, 1358 |
| FaqI | GGGAC | 2 cut(s) | 893, 1308 |
| FatI | CATG | 5 cut(s) | 109, 757, 791, 1054, 1354 |
| FauI | CCCGC | 1 cut(s) | 1069 |
| FauNDI | CATATG | 1 cut(s) | 561 |
| FblI | GTMKAC | 1 cut(s) | 1038 |
| Fnu4HI | GCNGC | 8 cut(s) | 341, 789, 798, 1363, 1395, 1415, 1459, 1468 |
| FokI | GGATG | 5 cut(s) | 203, 335, 434, 473, 908 |
| Fsp4HI | GCNGC | 8 cut(s) | 341, 789, 798, 1363, 1395, 1415, 1459, 1468 |
| FspBI | CTAG | 3 cut(s) | 47, 927, 1169 |
| GlaI | GCGC | 1 cut(s) | 23 |
| GluI | GCNGC | 8 cut(s) | 341, 789, 798, 1363, 1395, 1415, 1459, 1468 |
| GsuI | CTGGAG | 1 cut(s) | 151 |
| HaeIII | GGCC | 3 cut(s) | 329, 765, 1362 |
| HapII | CCGG | 3 cut(s) | 230, 1013, 1151 |
| HhaI | GCGC | 1 cut(s) | 24 |
| Hin1II | CATG | 5 cut(s) | 113, 761, 795, 1058, 1358 |
| Hin6I | GCGC | 1 cut(s) | 22 |
| HinP1I | GCGC | 1 cut(s) | 22 |
| HincII | GTYRAC | 2 cut(s) | 414, 1039 |
| HindII | GTYRAC | 2 cut(s) | 414, 1039 |
| HindIII | AAGCTT | 1 cut(s) | 817 |
| HpaII | CCGG | 3 cut(s) | 230, 1013, 1151 |
| HphI | GGTGA | 4 cut(s) | 108, 922, 1327, 1360 |
| Hpy166II | GTNNAC | 3 cut(s) | 414, 669, 1039 |
| Hpy188I | TCNGA | 2 cut(s) | 705, 918 |
| Hpy188III | TCNNGA | 6 cut(s) | 80, 164, 612, 662, 927, 1151 |
| Hpy8I | GTNNAC | 3 cut(s) | 414, 669, 1039 |
| Hpy99I | CGWCG | 1 cut(s) | 1043 |
| HpyCH4III | ACNGT | 1 cut(s) | 252 |
| HpyCH4IV | ACGT | 1 cut(s) | 1283 |
| HpyCH4V | TGCA | 9 cut(s) | 622, 715, 791, 899, 1007, 1327, 1414, 1467, 1504 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 14, 794, 797, 1121, 1368, 1400, 1464, 1467 |
| HpyF3I | CTNAG | 2 cut(s) | 702, 1259 |
| HpySE526I | ACGT | 1 cut(s) | 1283 |
| Hsp92II | CATG | 5 cut(s) | 113, 761, 795, 1058, 1358 |
| HspAI | GCGC | 1 cut(s) | 22 |
| Kpn2I | TCCGGA | 1 cut(s) | 1150 |
| Kzo9I | GATC | 2 cut(s) | 868, 1051 |
| LmnI | GCTCC | 4 cut(s) | 503, 1073, 1121, 1153 |
| Lsp1109I | GCAGC | 5 cut(s) | 775, 1406, 1426, 1445, 1479 |
| LweI | GCATC | 2 cut(s) | 886, 1225 |
| MaeI | CTAG | 3 cut(s) | 47, 927, 1169 |
| MaeII | ACGT | 1 cut(s) | 1283 |
| MaeIII | GTNAC | 4 cut(s) | 280, 1057, 1315, 1348 |
| MalI | GATC | 2 cut(s) | 870, 1053 |
| MbiI | CCGCTC | 1 cut(s) | 1076 |
| MboI | GATC | 2 cut(s) | 868, 1051 |
| MboII | GAAGA | 7 cut(s) | 33, 207, 462, 529, 532, 830, 1039 |
| MhlI | GDGCHC | 2 cut(s) | 508, 1158 |
| MluCI | AATT | 5 cut(s) | 207, 220, 300, 466, 1190 |
| MlyI | GAGTC | 6 cut(s) | 397, 617, 638, 674, 1324, 1345 |
| MmeI | TCCRAC | 2 cut(s) | 585, 836 |
| MroI | TCCGGA | 1 cut(s) | 1150 |
| MroXI | GAANNNNTTC | 1 cut(s) | 211 |
| MseI | TTAA | 6 cut(s) | 33, 299, 375, 480, 600, 1032 |
| MspA1I | CMGCKG | 3 cut(s) | 1258, 1458, 1470 |
| MspI | CCGG | 3 cut(s) | 230, 1013, 1151 |
| MspR9I | CCNGG | 2 cut(s) | 130, 1367 |
| Mva1269I | GAATGC | 1 cut(s) | 1046 |
| MvaI | CCWGG | 2 cut(s) | 130, 1367 |
| MvnI | CGCG | 1 cut(s) | 24 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 14, 794, 797, 1121, 1368, 1400, 1464, 1467 |
| NdeI | CATATG | 1 cut(s) | 561 |
| NdeII | GATC | 2 cut(s) | 868, 1051 |
| NheI | GCTAGC | 1 cut(s) | 1168 |
| NlaIII | CATG | 5 cut(s) | 113, 761, 795, 1058, 1358 |
| NlaIV | GGNNCC | 1 cut(s) | 881 |
| NmeAIII | GCCGAG | 1 cut(s) | 791 |
| NmuCI | GTSAC | 3 cut(s) | 1057, 1315, 1348 |
| NspI | RCATGY | 1 cut(s) | 795 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1047 |
| PctI | GAATGC | 1 cut(s) | 1046 |
| PdmI | GAANNNNTTC | 1 cut(s) | 211 |
| PfeI | GAWTC | 4 cut(s) | 226, 556, 913, 1087 |
| PflFI | GACNNNGTC | 1 cut(s) | 922 |
| PfoI | TCCNGGA | 1 cut(s) | 128 |
| PkrI | GCNGC | 8 cut(s) | 342, 790, 799, 1364, 1396, 1416, 1460, 1469 |
| PleI | GAGTC | 6 cut(s) | 396, 616, 637, 673, 1324, 1345 |
| PpsI | GAGTC | 6 cut(s) | 396, 616, 637, 673, 1324, 1345 |
| PshBI | ATTAAT | 2 cut(s) | 375, 1032 |
| Psp6I | CCWGG | 2 cut(s) | 128, 1365 |
| PspGI | CCWGG | 2 cut(s) | 128, 1365 |
| PspN4I | GGNNCC | 1 cut(s) | 881 |
| PspPI | GGNCC | 3 cut(s) | 749, 880, 1201 |
| PsyI | GACNNNGTC | 1 cut(s) | 922 |
| PvuII | CAGCTG | 2 cut(s) | 1258, 1458 |
| SalI | GTCGAC | 1 cut(s) | 1037 |
| SaqAI | TTAA | 6 cut(s) | 33, 299, 375, 480, 600, 1032 |
| SatI | GCNGC | 8 cut(s) | 341, 789, 798, 1363, 1395, 1415, 1459, 1468 |
| Sau3AI | GATC | 2 cut(s) | 868, 1051 |
| Sau96I | GGNCC | 3 cut(s) | 749, 880, 1201 |
| SchI | GAGTC | 6 cut(s) | 397, 617, 638, 674, 1324, 1345 |
| ScrFI | CCNGG | 2 cut(s) | 130, 1367 |
| SduI | GDGCHC | 2 cut(s) | 508, 1158 |
| SfaNI | GCATC | 2 cut(s) | 886, 1225 |
| SinI | GGWCC | 3 cut(s) | 749, 880, 1201 |
| Sse9I | AATT | 5 cut(s) | 207, 220, 300, 466, 1190 |
| SsiI | CCGC | 7 cut(s) | 340, 797, 800, 997, 1076, 1363, 1470 |
| SspI | AATATT | 1 cut(s) | 379 |
| SspMI | CTAG | 3 cut(s) | 47, 927, 1169 |
| StyD4I | CCNGG | 2 cut(s) | 128, 1365 |
| StyI | CCWWGG | 1 cut(s) | 417 |
| TaaI | ACNGT | 1 cut(s) | 252 |
| TaiI | ACGT | 1 cut(s) | 1286 |
| TaqI | TCGA | 6 cut(s) | 350, 391, 443, 611, 1038, 1050 |
| TasI | AATT | 5 cut(s) | 207, 220, 300, 466, 1190 |
| TauI | GCSGC | 3 cut(s) | 343, 800, 1365 |
| TfiI | GAWTC | 4 cut(s) | 226, 556, 913, 1087 |
| Tru1I | TTAA | 6 cut(s) | 33, 299, 375, 480, 600, 1032 |
| Tru9I | TTAA | 6 cut(s) | 33, 299, 375, 480, 600, 1032 |
| TscAI | CASTG | 2 cut(s) | 369, 1141 |
| TseFI | GTSAC | 3 cut(s) | 1057, 1315, 1348 |
| TseI | GCWGC | 5 cut(s) | 788, 1394, 1414, 1458, 1467 |
| Tsp45I | GTSAC | 3 cut(s) | 1057, 1315, 1348 |
| TspDTI | ATGAA | 4 cut(s) | 548, 774, 938, 1110 |
| TspGWI | ACGGA | 1 cut(s) | 1276 |
| TspRI | CASTG | 2 cut(s) | 369, 1141 |
| Tth111I | GACNNNGTC | 1 cut(s) | 922 |
| VpaK11BI | GGWCC | 3 cut(s) | 749, 880, 1201 |
| VspI | ATTAAT | 2 cut(s) | 375, 1032 |
| XapI | RAATTY | 2 cut(s) | 207, 1190 |
| XbaI | TCTAGA | 1 cut(s) | 926 |
| XceI | RCATGY | 1 cut(s) | 795 |
| XmiI | GTMKAC | 1 cut(s) | 1038 |
| XmnI | GAANNNNTTC | 1 cut(s) | 211 |
| XspI | CTAG | 3 cut(s) | 47, 927, 1169 |