Rw7G024400

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000996
Physical Location & Seq
Reverse (-)
29965283 .. 29968768
3486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G024400.1

Sequence Viewer

Length: 1197 bp
ATGGGTTATGCAAGATTAGTGCAGTGGTTGAATCTTCTAGTATGTTCAGTGATAATGCTGAAAGCAACCGGAGTTCCTGTCGGAATCACTATCCTTAAGACTGCGGTCGCCAGAGGAGCCGTTTGTTTGGATGGAAGCCCCCCAGCTTACCATTGGGATAAGGGGTTTGGTTCAGGAGTTAACAATTGGTTGGTTCACATTGAGGGAGGAGGATGGTGTCACAATGTCACAAATTGCCTTGGCCGTACGAACACTCGTTTAGGTTCATCAAAGAAAATGCTGAAGGAAATTCCTTTTTCTGGAATTCTAAGCAACAGGGAATCACTTAATCCAGGCTTTTACAATTGGAATAGAATCAAAGTCAGGTACTGCGATGGGTCATCATTCACTGGAGATGTTGAAGCAGTACACCCAGCTACTGGTCTTTACTTTAGAGGAGCTAGGGTTTTTCGAGCCGTTATTGCTGATTTATTAGCAAAAGGAATGGGGAAAGCTGAAAATGCTGTCCTCTCCGGTTGTTCTGCTGGTGGATTGGCCTCCATTCTTCATTGTGATAACTTCCGTGCTCTCTTACCTTCCGGAACCATTGTGAAATGTGTTTCAGATGCTGGTTACTTTATTAATGTAAAGGACATTTCTGGAGCACAACACATTCAACAGTATTTTGCTGATGTTGTTACAACACATGGTTCTGCGAAGAATTTGCCTCCATATTGCACTTCAAAATTGAGACCAGATTTGTGTTTCTTCCCTCAATATGTGGTACCACAGATTCGGACACCAATATTCTATGTACATTCAGCCTATGATTCATGGCAGATAAGGAACATTTTGGTACCAGGTGTTGCGGATCCTCATGGAACATGGCGAAACTGCAAGCTCAATATAAAGAACTGCTCACCCGCTCAGTTCAAAACCATTGAAAGCTTCAGGTTGCAATTTTTAACTGCAATTGGTGGTGTGAGCAACTCTGCAGTAAAGGGAATGTTCATAGACTCTTGCTATCTACACTGTCAAACTGAAATGCAGGAGACTTGGTTAATGCCTAATTCTCCACTCCTTAACAAAATGACAATTGCAGGGGCAGTTAGAGACTGGTTTTATGACCGAACTCCATTCCAAAAGATAGACTGTCCTTACTCTTGCAATCCCACTTGCCATCCTCCGATGTTTGATCCAAATGTGCACTTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

398

Amino Acids

43.93

Weight (kDa)

8.9

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 22 - 387 5.2e-171 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 763, 835
AccB1I GGYRCC 2 cut(s) 763, 835
AccB7I CCANNNNNTGG 1 cut(s) 419
AccBSI CCGCTC 1 cut(s) 905
AccIII TCCGGA 1 cut(s) 578
AciI CCGC 3 cut(s) 104, 848, 903
AclWI GGATC 3 cut(s) 845, 858, 1169
AcoI YGGCCR 1 cut(s) 241
AcsI RAATTY 3 cut(s) 288, 303, 700
AcuI CTGAAG 2 cut(s) 302, 913
AfaI GTAC 6 cut(s) 247, 368, 408, 765, 795, 837
AfiI CCNNNNNNNGG 2 cut(s) 299, 419
AflII CTTAAG 1 cut(s) 95
AgsI TTSAA 6 cut(s) 31, 401, 656, 723, 913, 923
AjnI CCWGG 2 cut(s) 331, 838
AluBI AGCT 6 cut(s) 146, 416, 440, 494, 880, 927
AluI AGCT 6 cut(s) 146, 416, 440, 494, 880, 927
Alw21I GWGCWC 3 cut(s) 568, 646, 1188
Alw26I GTCTC 3 cut(s) 724, 1025, 1086
Alw44I GTGCAC 1 cut(s) 1184
AlwI GGATC 3 cut(s) 845, 858, 1169
AlwNI CAGNNNCTG 3 cut(s) 369, 419, 608
Aor13HI TCCGGA 1 cut(s) 578
AoxI GGCC 2 cut(s) 241, 534
ApaLI GTGCAC 1 cut(s) 1184
ApoI RAATTY 3 cut(s) 288, 303, 700
AseI ATTAAT 1 cut(s) 621
Asp718I GGTACC 2 cut(s) 763, 835
AsuHPI GGTGA 1 cut(s) 891
BaeGI GKGCMC 1 cut(s) 1188
BamHI GGATCC 1 cut(s) 850
BanI GGYRCC 2 cut(s) 763, 835
Bbv12I GWGCWC 3 cut(s) 568, 646, 1188
BccI CCATC 4 cut(s) 125, 207, 368, 1167
BceAI ACGGC 3 cut(s) 104, 228, 440
BcgI CGANNNNNNTGC 2 cut(s) 685, 719
BciT130I CCWGG 2 cut(s) 333, 840
BcoDI GTCTC 3 cut(s) 724, 1025, 1086
BfaI CTAG 2 cut(s) 38, 441
BfmI CTRYAG 1 cut(s) 972
BfrI CTTAAG 1 cut(s) 95
Bme1390I CCNGG 2 cut(s) 333, 840
BmiI GGNNCC 5 cut(s) 118, 583, 765, 837, 852
BmrFI CCNGG 2 cut(s) 333, 840
BmsI GCATC 1 cut(s) 595
BoxI GACNNNNGTC 1 cut(s) 104
BpmI CTGGAG 2 cut(s) 411, 660
BsaI GGTCTC 1 cut(s) 724
BsaJI CCNNGG 1 cut(s) 238
BsaWI WCCGGW 3 cut(s) 68, 512, 578
BsaXI ACNNNNNCTCC 6 cut(s) 63, 93, 429, 459, 1022, 1052
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 419
Bse1I ACTGG 3 cut(s) 394, 424, 1100
BseAI TCCGGA 1 cut(s) 578
BseBI CCWGG 2 cut(s) 333, 840
BseDI CCNNGG 1 cut(s) 238
BseGI GGATG 3 cut(s) 136, 218, 1159
BseLI CCNNNNNNNGG 2 cut(s) 299, 419
BseMII CTCAG 1 cut(s) 920
BseNI ACTGG 3 cut(s) 394, 424, 1100
BseRI GAGGAG 3 cut(s) 129, 222, 450
BseSI GKGCMC 1 cut(s) 1188
BseYI CCCAGC 2 cut(s) 142, 412
BsgI GTGCAG 1 cut(s) 41
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 2 cut(s) 243, 536
BshNI GGYRCC 2 cut(s) 763, 835
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 3 cut(s) 568, 646, 1188
BsiSI CCGG 3 cut(s) 69, 513, 579
BsiWI CGTACG 1 cut(s) 245
BslI CCNNNNNNNGG 2 cut(s) 299, 419
BsmAI GTCTC 3 cut(s) 724, 1025, 1086
BsnI GGCC 2 cut(s) 243, 536
Bso31I GGTCTC 1 cut(s) 724
Bsp1286I GDGCHC 3 cut(s) 568, 646, 1188
Bsp13I TCCGGA 1 cut(s) 578
Bsp1407I TGTACA 1 cut(s) 793
Bsp143I GATC 2 cut(s) 850, 1174
BspACI CCGC 3 cut(s) 104, 848, 903
BspANI GGCC 2 cut(s) 243, 536
BspCNI CTCAG 1 cut(s) 919
BspEI TCCGGA 1 cut(s) 578
BspLI GGNNCC 5 cut(s) 118, 583, 765, 837, 852
BspMAI CTGCAG 1 cut(s) 976
BspPI GGATC 3 cut(s) 845, 858, 1169
BspT107I GGYRCC 2 cut(s) 763, 835
BspTI CTTAAG 1 cut(s) 95
BspTNI GGTCTC 1 cut(s) 724
BsrBI CCGCTC 1 cut(s) 905
BsrGI TGTACA 1 cut(s) 793
BsrI ACTGG 3 cut(s) 394, 424, 1100
BssECI CCNNGG 1 cut(s) 238
BssMI GATC 2 cut(s) 850, 1174
BssT1I CCWWGG 1 cut(s) 238
Bst2UI CCWGG 2 cut(s) 333, 840
Bst4CI ACNGT 3 cut(s) 660, 1013, 1133
BstAFI CTTAAG 1 cut(s) 95
BstAUI TGTACA 1 cut(s) 793
BstC8I GCNNGC 1 cut(s) 878
BstDEI CTNAG 2 cut(s) 308, 906
BstF5I GGATG 3 cut(s) 136, 218, 1159
BstKTI GATC 2 cut(s) 853, 1177
BstMAI GTCTC 3 cut(s) 724, 1025, 1086
BstMBI GATC 2 cut(s) 850, 1174
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 3 cut(s) 116, 461, 500
BstNI CCWGG 2 cut(s) 333, 840
BstPAI GACNNNNGTC 1 cut(s) 104
BstSCI CCNGG 2 cut(s) 331, 838
BstSFI CTRYAG 1 cut(s) 972
BstSLI GKGCMC 1 cut(s) 1188
BstX2I RGATCY 1 cut(s) 850
BstYI RGATCY 1 cut(s) 850
BsuRI GGCC 2 cut(s) 243, 536
BtgZI GCGATG 1 cut(s) 387
BtsCI GGATG 3 cut(s) 136, 218, 1159
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 4 cut(s) 29, 54, 387, 1009
Cac8I GCNNGC 1 cut(s) 878
CaiI CAGNNNCTG 3 cut(s) 369, 419, 608
CsiI ACCWGGT 1 cut(s) 838
Csp6I GTAC 6 cut(s) 246, 367, 407, 764, 794, 836
CviAII CATG 4 cut(s) 686, 813, 857, 864
CviQI GTAC 6 cut(s) 246, 367, 407, 764, 794, 836
DdeI CTNAG 2 cut(s) 308, 906
DpnI GATC 2 cut(s) 852, 1176
DpnII GATC 2 cut(s) 850, 1174
EaeI YGGCCR 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 238
Eco31I GGTCTC 1 cut(s) 724
Eco57I CTGAAG 2 cut(s) 302, 913
EcoRI GAATTC 1 cut(s) 303
EcoRII CCWGG 2 cut(s) 331, 838
EcoT14I CCWWGG 1 cut(s) 238
ErhI CCWWGG 1 cut(s) 238
FaeI CATG 4 cut(s) 689, 816, 860, 867
FatI CATG 4 cut(s) 685, 812, 856, 863
FauI CCCGC 1 cut(s) 910
FokI GGATG 3 cut(s) 143, 225, 1146
FspBI CTAG 2 cut(s) 38, 441
GsaI CCCAGC 2 cut(s) 146, 416
GsuI CTGGAG 2 cut(s) 411, 660
HaeIII GGCC 2 cut(s) 243, 536
HapII CCGG 3 cut(s) 69, 513, 579
Hin1II CATG 4 cut(s) 689, 816, 860, 867
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 925
HinfI GANTC 7 cut(s) 31, 84, 320, 354, 772, 809, 995
HpaI GTTAAC 1 cut(s) 181
HpaII CCGG 3 cut(s) 69, 513, 579
HphI GGTGA 1 cut(s) 891
Hpy166II GTNNAC 4 cut(s) 181, 196, 409, 1186
Hpy188I TCNGA 4 cut(s) 83, 604, 777, 1167
Hpy188III TCNNGA 4 cut(s) 174, 300, 579, 639
Hpy8I GTNNAC 4 cut(s) 181, 196, 409, 1186
HpyAV CCTTC 2 cut(s) 277, 585
HpyCH4III ACNGT 3 cut(s) 660, 1013, 1133
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 461, 500
HpyF3I CTNAG 2 cut(s) 308, 906
Hsp92II CATG 4 cut(s) 689, 816, 860, 867
Kpn2I TCCGGA 1 cut(s) 578
KpnI GGTACC 2 cut(s) 767, 839
KspAI GTTAAC 1 cut(s) 181
Kzo9I GATC 2 cut(s) 850, 1174
LmnI GCTCC 3 cut(s) 116, 437, 641
LweI GCATC 1 cut(s) 595
MabI ACCWGGT 1 cut(s) 838
MaeI CTAG 2 cut(s) 38, 441
MaeIII GTNAC 4 cut(s) 218, 226, 611, 676
MalI GATC 2 cut(s) 852, 1176
MbiI CCGCTC 1 cut(s) 905
MboI GATC 2 cut(s) 850, 1174
MboII GAAGA 4 cut(s) 26, 536, 709, 739
MfeI CAATTG 4 cut(s) 184, 343, 951, 1074
MflI RGATCY 1 cut(s) 850
MhlI GDGCHC 3 cut(s) 568, 646, 1188
MlyI GAGTC 1 cut(s) 989
MmeI TCCRAC 1 cut(s) 61
MroI TCCGGA 1 cut(s) 578
MseI TTAA 7 cut(s) 96, 180, 327, 621, 944, 1040, 1062
MspCI CTTAAG 1 cut(s) 95
MspI CCGG 3 cut(s) 69, 513, 579
MspR9I CCNGG 2 cut(s) 333, 840
MunI CAATTG 4 cut(s) 184, 343, 951, 1074
MvaI CCWGG 2 cut(s) 333, 840
MwoI GCNNNNNNNGC 3 cut(s) 116, 461, 500
NdeII GATC 2 cut(s) 850, 1174
NlaIII CATG 4 cut(s) 689, 816, 860, 867
NlaIV GGNNCC 5 cut(s) 118, 583, 765, 837, 852
NmuCI GTSAC 2 cut(s) 218, 226
PfeI GAWTC 6 cut(s) 31, 84, 320, 354, 772, 809
Pfl23II CGTACG 1 cut(s) 245
PflMI CCANNNNNTGG 1 cut(s) 419
PleI GAGTC 1 cut(s) 989
PpsI GAGTC 1 cut(s) 989
PshAI GACNNNNGTC 1 cut(s) 104
PshBI ATTAAT 1 cut(s) 621
Psp6I CCWGG 2 cut(s) 331, 838
PspFI CCCAGC 2 cut(s) 142, 412
PspGI CCWGG 2 cut(s) 331, 838
PspLI CGTACG 1 cut(s) 245
PspN4I GGNNCC 5 cut(s) 118, 583, 765, 837, 852
PstI CTGCAG 1 cut(s) 976
PstNI CAGNNNCTG 3 cut(s) 369, 419, 608
PsuI RGATCY 1 cut(s) 850
RsaI GTAC 6 cut(s) 247, 368, 408, 765, 795, 837
RsaNI GTAC 6 cut(s) 246, 367, 407, 764, 794, 836
SaqAI TTAA 7 cut(s) 96, 180, 327, 621, 944, 1040, 1062
Sau3AI GATC 2 cut(s) 850, 1174
SchI GAGTC 1 cut(s) 989
ScrFI CCNGG 2 cut(s) 333, 840
SduI GDGCHC 3 cut(s) 568, 646, 1188
SexAI ACCWGGT 1 cut(s) 838
SfaNI GCATC 1 cut(s) 595
SfcI CTRYAG 1 cut(s) 972
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SsiI CCGC 3 cut(s) 104, 848, 903
SspI AATATT 1 cut(s) 786
SspMI CTAG 2 cut(s) 38, 441
StyD4I CCNGG 2 cut(s) 331, 838
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 3 cut(s) 660, 1013, 1133
TaqI TCGA 1 cut(s) 451
TaqII GACCGA 1 cut(s) 1122
TatI WGTACW 2 cut(s) 406, 793
TfiI GAWTC 6 cut(s) 31, 84, 320, 354, 772, 809
Tru1I TTAA 7 cut(s) 96, 180, 327, 621, 944, 1040, 1062
Tru9I TTAA 7 cut(s) 96, 180, 327, 621, 944, 1040, 1062
TscAI CASTG 4 cut(s) 29, 54, 394, 1016
TseFI GTSAC 2 cut(s) 218, 226
Tsp45I GTSAC 2 cut(s) 218, 226
TspDTI ATGAA 4 cut(s) 255, 536, 801, 979
TspGWI ACGGA 1 cut(s) 551
TspRI CASTG 4 cut(s) 29, 54, 394, 1016
Van91I CCANNNNNTGG 1 cut(s) 419
Vha464I CTTAAG 1 cut(s) 95
VneI GTGCAC 1 cut(s) 1184
VspI ATTAAT 1 cut(s) 621
XapI RAATTY 3 cut(s) 288, 303, 700
XcmI CCANNNNNNNNNTGG 1 cut(s) 150
XspI CTAG 2 cut(s) 38, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.