Prupe.5G015300_v2.0.a1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000996
Physical Location & Seq
Forward (+)
1682302 .. 1686857
4556 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G015300.1

Sequence Viewer

Length: 1203 bp
ATGGCTGATGCAAGATTAGGCCAATGGTTATTTCTTCTAGCATGTGCAGTCACACTGCTGAAAGCAGCAGGAGTTCCTGTGGAAATAACTTATGTTCATAGTGCGATCGCAAAAGGAGCTGTTTGTTTGGATGGGAGTCCCCCAGCTTACCACCATGATAAGGGATTTGGCTCAGGAATTAACAATTGGTTGGTTCACATTGAGGGAGGAGGATGGTGTAACAATGTCACAAATTGCCTTGGCCGTAAAAACACTCGTTTAGGTTCATCTAAGCAAATGCTGAAGGCAATTGCCTTTTCTGGGATTCTGAGCAACAGGAAAATGTTTAATCCAGACTTTTACAATTGGAACAGAATCAAAGTTAGGTACTGCGATGGGGCATCATTCACTGGTGATGTTGCAGCAGTAAACCCAGCTACTGGTCTTTACTTCAGAGGAGCAAGGATCTTTCGTGCCGTCATTGAAGATCTATTAGCAAAAGGAATGGGGAACGCTCAAAATGCTATTCTCTCTGGTTGTTCTGCCGGTGGATTGACTTCTATTCTTCATTGCGACAACTTCCATGCTCTCTTGCCTGCGGGAACGAATGTCAAATGTGTTTCAGACGCTGGTTACTTTATTAATGTAAAGGATGTTTCTGGAGCCCAACACATCGAAGAGTTATTTAGTCAAGTGGTTGAAACACATGGTTCAGCCAGGAATTTGCCTCCATCCTGCACTTCAAAATTGAGACCAGGTTTGTGCTTCTTCCCGCAATATATGGCATCCCAAATCCAGACGCGGATATTCTTTGTAAACGCAGCCTACGATTCATGGCAGATCAAGAACATTTTGGCACCGGGAGTTGCCGATCCTCATGGCACTTGGCACGAGTGCAAGCTCGATATAAAGAATTGCTCACCCACTCAACTCAAAGCCATGCAAGATTTCAGGGTGCAGTTTTTAAGTGCAGTTGGTGGCATGACCGGCTGTCCATTAAAAGGAATGTTCATAGACTCTTGCTATGCTCACTGTCAAACTGAAATGCAGGAGACGTGGTTAATGGCTGATTCTCCGGTGCTCAACAAGACGACAATTGCACAGGCAGTTGGCGACTGGTTCTATGGCAGAACTCCGTTCCAAAAGATAGATTGCCCTTACCCTTGCAACCCCTCTTGCCATAACCGTGTTTTTGATCCGAACCAGCACCTACTAGAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

401

Amino Acids

43.79

Weight (kDa)

8.37

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 835
AccB7I CCANNNNNTGG 1 cut(s) 419
AccII CGCG 1 cut(s) 781
AciI CCGC 3 cut(s) 578, 752, 781
AclWI GGATC 3 cut(s) 452, 845, 1169
AcoI YGGCCR 1 cut(s) 241
AcsI RAATTY 1 cut(s) 700
AcuI CTGAAG 2 cut(s) 302, 415
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 4 cut(s) 160, 300, 419, 980
AgsI TTSAA 3 cut(s) 464, 680, 723
AhdI GACNNNNNGTC 1 cut(s) 969
AjiI CACGTC 1 cut(s) 1035
AjnI CCWGG 2 cut(s) 695, 733
AjuI GAANNNNNNNTTGG 4 cut(s) 15, 47, 169, 201
AluBI AGCT 4 cut(s) 119, 146, 416, 880
AluI AGCT 4 cut(s) 119, 146, 416, 880
Alw21I GWGCWC 1 cut(s) 1062
Alw26I GTCTC 2 cut(s) 724, 1025
AlwI GGATC 3 cut(s) 452, 845, 1169
AlwNI CAGNNNCTG 2 cut(s) 419, 608
AoxI GGCC 2 cut(s) 19, 241
ApeKI GCWGC 3 cut(s) 65, 401, 800
ApoI RAATTY 1 cut(s) 700
AseI ATTAAT 1 cut(s) 621
AsiSI GCGATCGC 1 cut(s) 108
AsuC2I CCSGG 1 cut(s) 840
AsuHPI GGTGA 2 cut(s) 404, 891
BanI GGYRCC 1 cut(s) 835
BanII GRGCYC 1 cut(s) 646
BauI CACGAG 1 cut(s) 869
Bbv12I GWGCWC 1 cut(s) 1062
BbvI GCAGC 3 cut(s) 77, 413, 812
BccI CCATC 4 cut(s) 125, 207, 368, 718
BceAI ACGGC 2 cut(s) 228, 440
BciT130I CCWGG 2 cut(s) 697, 735
BcnI CCSGG 1 cut(s) 840
BcoDI GTCTC 2 cut(s) 724, 1025
BfaI CTAG 2 cut(s) 38, 1193
BglII AGATCT 1 cut(s) 466
BisI GCNGC 3 cut(s) 66, 402, 801
BlsI GCNGC 3 cut(s) 67, 403, 802
Bme1390I CCNGG 3 cut(s) 697, 735, 840
BmeRI GACNNNNNGTC 1 cut(s) 969
BmgBI CACGTC 1 cut(s) 1035
BmiI GGNNCC 2 cut(s) 643, 837
BmrFI CCNGG 3 cut(s) 697, 735, 840
BmsI GCATC 2 cut(s) 389, 773
BpmI CTGGAG 1 cut(s) 660
Bpu10I CCTNAGC 1 cut(s) 172
BpuMI CCSGG 1 cut(s) 840
BsaI GGTCTC 1 cut(s) 724
BsaJI CCNNGG 1 cut(s) 238
BsaWI WCCGGW 1 cut(s) 1054
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 1022, 1052
Bsc4I CCNNNNNNNGG 4 cut(s) 160, 300, 419, 980
Bse118I RCCGGY 2 cut(s) 524, 965
Bse1I ACTGG 3 cut(s) 394, 424, 1100
Bse3DI GCAATG 1 cut(s) 547
BseBI CCWGG 2 cut(s) 697, 735
BseDI CCNNGG 1 cut(s) 238
BseGI GGATG 5 cut(s) 136, 218, 637, 710, 764
BseLI CCNNNNNNNGG 4 cut(s) 160, 300, 419, 980
BseMI GCAATG 1 cut(s) 547
BseMII CTCAG 2 cut(s) 186, 299
BseNI ACTGG 3 cut(s) 394, 424, 1100
BseRI GAGGAG 2 cut(s) 222, 450
BseXI GCAGC 3 cut(s) 77, 413, 812
BseYI CCCAGC 2 cut(s) 142, 412
BsgI GTGCAG 4 cut(s) 66, 700, 956, 969
Bsh1236I CGCG 1 cut(s) 781
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 2 cut(s) 21, 243
BshNI GGYRCC 1 cut(s) 835
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 1062
BsiSI CCGG 4 cut(s) 525, 839, 966, 1055
BslFI GGGAC 1 cut(s) 123
BslI CCNNNNNNNGG 4 cut(s) 160, 300, 419, 980
BsmAI GTCTC 2 cut(s) 724, 1025
BsmBI CGTCTC 1 cut(s) 1025
BsmFI GGGAC 1 cut(s) 123
BsnI GGCC 2 cut(s) 21, 243
Bso31I GGTCTC 1 cut(s) 724
Bsp1286I GDGCHC 2 cut(s) 646, 1062
Bsp143I GATC 6 cut(s) 105, 444, 466, 819, 850, 1174
BspACI CCGC 3 cut(s) 578, 752, 781
BspANI GGCC 2 cut(s) 21, 243
BspCNI CTCAG 2 cut(s) 185, 300
BspFNI CGCG 1 cut(s) 781
BspLI GGNNCC 2 cut(s) 643, 837
BspPI GGATC 3 cut(s) 452, 845, 1169
BspT107I GGYRCC 1 cut(s) 835
BspTNI GGTCTC 1 cut(s) 724
BsrDI GCAATG 1 cut(s) 547
BsrFI RCCGGY 2 cut(s) 524, 965
BsrI ACTGG 3 cut(s) 394, 424, 1100
BssAI RCCGGY 2 cut(s) 524, 965
BssECI CCNNGG 1 cut(s) 238
BssMI GATC 6 cut(s) 105, 444, 466, 819, 850, 1174
BssSI CACGAG 1 cut(s) 869
BssT1I CCWWGG 1 cut(s) 238
Bst2BI CACGAG 1 cut(s) 869
Bst2UI CCWGG 2 cut(s) 697, 735
Bst4CI ACNGT 2 cut(s) 1013, 1166
Bst6I CTCTTC 1 cut(s) 651
BstC8I GCNNGC 2 cut(s) 576, 878
BstDEI CTNAG 3 cut(s) 172, 270, 308
BstF5I GGATG 5 cut(s) 136, 218, 637, 710, 764
BstFNI CGCG 1 cut(s) 781
BstKTI GATC 6 cut(s) 108, 447, 469, 822, 853, 1177
BstMAI GTCTC 2 cut(s) 724, 1025
BstMBI GATC 6 cut(s) 105, 444, 466, 819, 850, 1174
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 3 cut(s) 116, 500, 966
BstNI CCWGG 2 cut(s) 697, 735
BstNSI RCATGY 1 cut(s) 45
BstSCI CCNGG 3 cut(s) 695, 733, 838
BstUI CGCG 1 cut(s) 781
BstV1I GCAGC 3 cut(s) 77, 413, 812
BstX2I RGATCY 2 cut(s) 444, 466
BstYI RGATCY 2 cut(s) 444, 466
BsuRI GGCC 2 cut(s) 21, 243
BtgZI GCGATG 1 cut(s) 387
BtrI CACGTC 1 cut(s) 1035
BtsCI GGATG 5 cut(s) 136, 218, 637, 710, 764
BtsI GCAGTG 1 cut(s) 53
BtsIMutI CAGTG 3 cut(s) 53, 387, 1009
Cac8I GCNNGC 2 cut(s) 576, 878
CaiI CAGNNNCTG 2 cut(s) 419, 608
Cfr10I RCCGGY 2 cut(s) 524, 965
CseI GACGC 2 cut(s) 614, 787
CsiI ACCWGGT 1 cut(s) 733
Csp6I GTAC 1 cut(s) 367
CviAII CATG 8 cut(s) 42, 155, 563, 686, 813, 857, 919, 961
CviQI GTAC 1 cut(s) 367
DdeI CTNAG 3 cut(s) 172, 270, 308
DpnI GATC 6 cut(s) 107, 446, 468, 821, 852, 1176
DpnII GATC 6 cut(s) 105, 444, 466, 819, 850, 1174
DriI GACNNNNNGTC 1 cut(s) 969
EaeI YGGCCR 1 cut(s) 241
Eam1104I CTCTTC 1 cut(s) 651
Eam1105I GACNNNNNGTC 1 cut(s) 969
EarI CTCTTC 1 cut(s) 651
Eco130I CCWWGG 1 cut(s) 238
Eco24I GRGCYC 1 cut(s) 646
Eco31I GGTCTC 1 cut(s) 724
Eco57I CTGAAG 2 cut(s) 302, 415
EcoRII CCWGG 2 cut(s) 695, 733
EcoT14I CCWWGG 1 cut(s) 238
EcoT38I GRGCYC 1 cut(s) 646
ErhI CCWWGG 1 cut(s) 238
Esp3I CGTCTC 1 cut(s) 1025
FaeI CATG 8 cut(s) 45, 158, 566, 689, 816, 860, 922, 964
FaqI GGGAC 1 cut(s) 123
FatI CATG 8 cut(s) 41, 154, 562, 685, 812, 856, 918, 960
FauI CCCGC 2 cut(s) 571, 759
Fnu4HI GCNGC 3 cut(s) 66, 402, 801
FokI GGATG 5 cut(s) 143, 225, 644, 697, 751
FriOI GRGCYC 1 cut(s) 646
Fsp4HI GCNGC 3 cut(s) 66, 402, 801
FspBI CTAG 2 cut(s) 38, 1193
GluI GCNGC 3 cut(s) 66, 402, 801
GsaI CCCAGC 2 cut(s) 146, 416
GsuI CTGGAG 1 cut(s) 660
HaeIII GGCC 2 cut(s) 21, 243
HapII CCGG 4 cut(s) 525, 839, 966, 1055
HgaI GACGC 2 cut(s) 614, 787
Hin1II CATG 8 cut(s) 45, 158, 566, 689, 816, 860, 922, 964
HinfI GANTC 6 cut(s) 136, 304, 354, 809, 995, 1049
HpaII CCGG 4 cut(s) 525, 839, 966, 1055
HphI GGTGA 2 cut(s) 404, 891
Hpy166II GTNNAC 3 cut(s) 196, 409, 796
Hpy188I TCNGA 4 cut(s) 309, 434, 604, 1179
Hpy188III TCNNGA 5 cut(s) 174, 332, 639, 775, 823
Hpy8I GTNNAC 3 cut(s) 196, 409, 796
HpyAV CCTTC 1 cut(s) 277
HpyCH4III ACNGT 2 cut(s) 1013, 1166
HpyCH4IV ACGT 1 cut(s) 1034
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 500, 966
HpyF3I CTNAG 3 cut(s) 172, 270, 308
HpySE526I ACGT 1 cut(s) 1034
Hsp92II CATG 8 cut(s) 45, 158, 566, 689, 816, 860, 922, 964
Kzo9I GATC 6 cut(s) 105, 444, 466, 819, 850, 1174
LmnI GCTCC 3 cut(s) 116, 437, 641
Lsp1109I GCAGC 3 cut(s) 77, 413, 812
LweI GCATC 2 cut(s) 389, 773
MabI ACCWGGT 1 cut(s) 733
MaeI CTAG 2 cut(s) 38, 1193
MaeII ACGT 1 cut(s) 1034
MaeIII GTNAC 4 cut(s) 49, 218, 226, 611
MalI GATC 6 cut(s) 107, 446, 468, 821, 852, 1176
MboI GATC 6 cut(s) 105, 444, 466, 819, 850, 1174
MboII GAAGA 5 cut(s) 26, 476, 536, 668, 739
MfeI CAATTG 4 cut(s) 184, 288, 343, 1074
MflI RGATCY 2 cut(s) 444, 466
MhlI GDGCHC 2 cut(s) 646, 1062
MluCI AATT 9 cut(s) 177, 184, 232, 288, 343, 700, 725, 892, 1074
MlyI GAGTC 2 cut(s) 145, 989
MnlI CCTC 7 cut(s) 196, 200, 203, 428, 717, 864, 1162
MseI TTAA 6 cut(s) 180, 327, 621, 944, 977, 1040
MslI CAYNNNNRTG 1 cut(s) 1164
MspI CCGG 4 cut(s) 525, 839, 966, 1055
MspR9I CCNGG 3 cut(s) 697, 735, 840
MunI CAATTG 4 cut(s) 184, 288, 343, 1074
MvaI CCWGG 2 cut(s) 697, 735
MvnI CGCG 1 cut(s) 781
MwoI GCNNNNNNNGC 3 cut(s) 116, 500, 966
NciI CCSGG 1 cut(s) 840
NdeII GATC 6 cut(s) 105, 444, 466, 819, 850, 1174
NlaIII CATG 8 cut(s) 45, 158, 566, 689, 816, 860, 922, 964
NlaIV GGNNCC 2 cut(s) 643, 837
NmuCI GTSAC 2 cut(s) 49, 226
NspI RCATGY 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 804
PfeI GAWTC 4 cut(s) 304, 354, 809, 1049
PflMI CCANNNNNTGG 1 cut(s) 419
PkrI GCNGC 3 cut(s) 67, 403, 802
Ple19I CGATCG 1 cut(s) 108
PleI GAGTC 2 cut(s) 144, 989
PpsI GAGTC 2 cut(s) 144, 989
PshBI ATTAAT 1 cut(s) 621
Psp6I CCWGG 2 cut(s) 695, 733
PspFI CCCAGC 2 cut(s) 142, 412
PspGI CCWGG 2 cut(s) 695, 733
PspN4I GGNNCC 2 cut(s) 643, 837
PstNI CAGNNNCTG 2 cut(s) 419, 608
PsuI RGATCY 2 cut(s) 444, 466
PvuI CGATCG 1 cut(s) 108
RgaI GCGATCGC 1 cut(s) 108
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
RseI CAYNNNNRTG 1 cut(s) 1164
SaqAI TTAA 6 cut(s) 180, 327, 621, 944, 977, 1040
SatI GCNGC 3 cut(s) 66, 402, 801
Sau3AI GATC 6 cut(s) 105, 444, 466, 819, 850, 1174
SchI GAGTC 2 cut(s) 145, 989
ScrFI CCNGG 3 cut(s) 697, 735, 840
SduI GDGCHC 2 cut(s) 646, 1062
SetI ASST 9 cut(s) 121, 148, 265, 368, 418, 739, 882, 1037, 1191
SexAI ACCWGGT 1 cut(s) 733
SfaAI GCGATCGC 1 cut(s) 108
SfaNI GCATC 2 cut(s) 389, 773
SgfI GCGATCGC 1 cut(s) 108
SmiMI CAYNNNNRTG 1 cut(s) 1164
Sse9I AATT 9 cut(s) 177, 184, 232, 288, 343, 700, 725, 892, 1074
SsiI CCGC 3 cut(s) 578, 752, 781
SspMI CTAG 2 cut(s) 38, 1193
StyD4I CCNGG 3 cut(s) 695, 733, 838
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 2 cut(s) 1013, 1166
TaiI ACGT 1 cut(s) 1037
TaqI TCGA 2 cut(s) 654, 882
TasI AATT 9 cut(s) 177, 184, 232, 288, 343, 700, 725, 892, 1074
TfiI GAWTC 4 cut(s) 304, 354, 809, 1049
Tru1I TTAA 6 cut(s) 180, 327, 621, 944, 977, 1040
Tru9I TTAA 6 cut(s) 180, 327, 621, 944, 977, 1040
TscAI CASTG 3 cut(s) 60, 394, 1016
TseFI GTSAC 2 cut(s) 49, 226
TseI GCWGC 3 cut(s) 65, 401, 800
Tsp45I GTSAC 2 cut(s) 49, 226
TspDTI ATGAA 5 cut(s) 86, 255, 536, 801, 979
TspGWI ACGGA 1 cut(s) 1104
TspRI CASTG 3 cut(s) 60, 394, 1016
Van91I CCANNNNNTGG 1 cut(s) 419
VspI ATTAAT 1 cut(s) 621
XapI RAATTY 1 cut(s) 700
XceI RCATGY 1 cut(s) 45
XspI CTAG 2 cut(s) 38, 1193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.