RLG00000002703

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000996
Physical Location & Seq
Forward (+)
38344162 .. 38348113
3952 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002703

Sequence Viewer

Length: 1392 bp
ATGAACCCGACAAGATTCGTTACAGCTCTACTAAGCTTTTTCACTGTGCAACAGCAGCTCCTCCGTCGGGCTATCCCACTCGGGGTCGGTTATCCAGTACACACAGTACAAACAACAACCCCATGTTCTGAACTGGGTTTCTCTCACATTCTTCATCACTCTGTCTCTGCCATTTTCTCTCCTTCATTCAGGCAGATGGGTGATGCAAGATTAGCGCAATGGTTGAATCTTCTAGTGTGTTCAGTGATACTGCTGAAAGCAATGGGGGTTCCTGTCGGAATCACTATCCTTAAGACTGCGGTCGGCAAAGGAGCCGTTTGTTTGGATGGGAGCCCCCCAGCTTACCATTGGGATAAGGGGTTTGGTTCAGGAGTTAACAATTGGTTGGTTCACATTGAGGGAGGAGGATGGTGTAACAATGTCACAAATTGCCTCGGCCGTAAGAACACTCGTTTAGGTTCATCAAAGCAAATGCTGAAGAAAATTCCTTTTTCTGGAATTCTGAGCAACAAGCAATCACTTAATCCAGGCTTTTACAATTGGAATAGAATCAAAGTCAGGTACTGCGATGGGTCATCATTCACTGGAGATGTTGAAGCAGTACACCAAGCTACTGGTCTTTACTTCAGAGGAGCAAGGGTTTTTCGAGCTGTTATTGCTGATTTATTAGCAAAAGGAATGGGGAAAGCTAAAAATGCTGTCCTCTCTGGTTGTTCTGCTGGTGGATTGACCACTATTCTTCATTGTGATAACTTCCGTGCTCTCTTACATTCTGGAACCATTGTGAAATGTGTTTCAGATGCTGGTTACTTTATTAATGTAAAGGACATTTCTGGAGCACAACACATTCAACAGTATTTTGCTGATGTTGTTACAACACATGGTTCTGCGAAGAATTTGCCTCCATCTTGCACTTCAAAATTGAGACCAGATTTGTGTTTCTTCCCTCAATATGTGGTACCAAAGATTCGGACACCAATATTCTATGTAAATGCAGCCTATGATTCATGGCAGATAAGGAACAGTTTGGTACCAAGTGTTGCGGATCCTCATGGAACTTGGCGAAACTGCAAGCTCAATATAAAGAACTGCTCACCCGCTCAGCTCAAAACCATTCAAAACTTCAGGTTGCAATTTTTAACTGCAATTGGTGGTGTGAGCAACTCCGCAGTAAAGGGAATGTTCATAGACTCTTGCTATCTACACTGTCAAACTGAATTGCAGGAGACTTGGTTAATGCCTAATTCTCCACTCCTTAACAAAATGACAATTGCAGGGGCAGTTAGAGACTGGTTTTATGACCGAACTCCATTCCAAAAGATAGACTGTCCTTACTCTTGCAATCCCACTTGCCATGCTCCGATGTTTGATCCAAATGTGCACTTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

464

Amino Acids

51.0

Weight (kDa)

9.34

Isoelectric Point (pI)

40.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 89 - 452 1.5e-172 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 958, 1030
AccB1I GGYRCC 2 cut(s) 958, 1030
AccBSI CCGCTC 1 cut(s) 1100
AciI CCGC 4 cut(s) 299, 1043, 1098, 1167
AclWI GGATC 3 cut(s) 1040, 1053, 1364
AcoI YGGCCR 1 cut(s) 436
AcsI RAATTY 3 cut(s) 483, 498, 895
AcuI CTGAAG 3 cut(s) 497, 610, 1108
AfaI GTAC 6 cut(s) 99, 108, 563, 603, 960, 1032
AfiI CCNNNNNNNGG 3 cut(s) 67, 82, 494
AflII CTTAAG 1 cut(s) 290
AgsI TTSAA 5 cut(s) 226, 596, 851, 918, 1118
AjnI CCWGG 1 cut(s) 526
AluBI AGCT 9 cut(s) 26, 36, 58, 341, 611, 650, 689, 1075, 1105
AluI AGCT 9 cut(s) 26, 36, 58, 341, 611, 650, 689, 1075, 1105
Alw21I GWGCWC 3 cut(s) 763, 841, 1383
Alw26I GTCTC 4 cut(s) 169, 919, 1220, 1281
Alw44I GTGCAC 1 cut(s) 1379
AlwI GGATC 3 cut(s) 1040, 1053, 1364
AlwNI CAGNNNCTG 2 cut(s) 564, 803
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 1 cut(s) 436
ApaLI GTGCAC 1 cut(s) 1379
ApeKI GCWGC 2 cut(s) 55, 995
ApoI RAATTY 3 cut(s) 483, 498, 895
AseI ATTAAT 1 cut(s) 816
Asp718I GGTACC 2 cut(s) 958, 1030
AspLEI GCGC 1 cut(s) 217
AsuHPI GGTGA 2 cut(s) 212, 1086
AvaI CYCGRG 1 cut(s) 80
BaeGI GKGCMC 1 cut(s) 1383
BamHI GGATCC 1 cut(s) 1045
BanI GGYRCC 2 cut(s) 958, 1030
BanII GRGCYC 1 cut(s) 335
Bbv12I GWGCWC 3 cut(s) 763, 841, 1383
BbvI GCAGC 2 cut(s) 67, 1007
BccI CCATC 5 cut(s) 190, 320, 402, 563, 913
BceAI ACGGC 2 cut(s) 299, 423
BcgI CGANNNNNNTGC 2 cut(s) 880, 914
BciT130I CCWGG 1 cut(s) 528
BcoDI GTCTC 4 cut(s) 169, 919, 1220, 1281
BfaI CTAG 1 cut(s) 233
BfrI CTTAAG 1 cut(s) 290
BisI GCNGC 2 cut(s) 56, 996
BlpI GCTNAGC 1 cut(s) 1101
BlsI GCNGC 2 cut(s) 57, 997
Bme1390I CCNGG 1 cut(s) 528
BmeT110I CYCGRG 1 cut(s) 80
BmiI GGNNCC 7 cut(s) 270, 313, 332, 778, 960, 1032, 1047
BmrFI CCNGG 1 cut(s) 528
BmrI ACTGGG 1 cut(s) 143
BmsI GCATC 2 cut(s) 193, 790
BmuI ACTGGG 1 cut(s) 143
BoxI GACNNNNGTC 1 cut(s) 299
BpmI CTGGAG 2 cut(s) 606, 855
Bpu1102I GCTNAGC 1 cut(s) 1101
BsaI GGTCTC 1 cut(s) 919
BsaJI CCNNGG 1 cut(s) 433
BsaXI ACNNNNNCTCC 6 cut(s) 42, 72, 624, 654, 1217, 1247
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 82, 494
Bse1I ACTGG 5 cut(s) 95, 138, 589, 619, 1295
Bse3DI GCAATG 2 cut(s) 224, 267
BseBI CCWGG 1 cut(s) 528
BseDI CCNNGG 1 cut(s) 433
BseGI GGATG 2 cut(s) 331, 413
BseLI CCNNNNNNNGG 3 cut(s) 67, 82, 494
BseMI GCAATG 2 cut(s) 224, 267
BseMII CTCAG 2 cut(s) 494, 1115
BseNI ACTGG 5 cut(s) 95, 138, 589, 619, 1295
BseRI GAGGAG 3 cut(s) 50, 417, 645
BseSI GKGCMC 1 cut(s) 1383
BseX3I CGGCCG 1 cut(s) 436
BseXI GCAGC 2 cut(s) 67, 1007
BseYI CCCAGC 1 cut(s) 337
Bsh1285I CGRYCG 2 cut(s) 303, 439
BshFI GGCC 1 cut(s) 438
BshNI GGYRCC 2 cut(s) 958, 1030
BsiEI CGRYCG 2 cut(s) 303, 439
BsiHKAI GWGCWC 3 cut(s) 763, 841, 1383
BsiHKCI CYCGRG 1 cut(s) 80
BslI CCNNNNNNNGG 3 cut(s) 67, 82, 494
BsmAI GTCTC 4 cut(s) 169, 919, 1220, 1281
BsnI GGCC 1 cut(s) 438
Bso31I GGTCTC 1 cut(s) 919
BsoBI CYCGRG 1 cut(s) 80
Bsp1286I GDGCHC 4 cut(s) 335, 763, 841, 1383
Bsp143I GATC 2 cut(s) 1045, 1369
Bsp1720I GCTNAGC 1 cut(s) 1101
BspACI CCGC 4 cut(s) 299, 1043, 1098, 1167
BspANI GGCC 1 cut(s) 438
BspCNI CTCAG 2 cut(s) 495, 1114
BspLI GGNNCC 7 cut(s) 270, 313, 332, 778, 960, 1032, 1047
BspPI GGATC 3 cut(s) 1040, 1053, 1364
BspT107I GGYRCC 2 cut(s) 958, 1030
BspTI CTTAAG 1 cut(s) 290
BspTNI GGTCTC 1 cut(s) 919
BsrBI CCGCTC 1 cut(s) 1100
BsrDI GCAATG 2 cut(s) 224, 267
BsrI ACTGG 5 cut(s) 95, 138, 589, 619, 1295
BssECI CCNNGG 1 cut(s) 433
BssMI GATC 2 cut(s) 1045, 1369
Bst2UI CCWGG 1 cut(s) 528
Bst4CI ACNGT 6 cut(s) 46, 106, 855, 1025, 1208, 1328
BstAFI CTTAAG 1 cut(s) 290
BstC8I GCNNGC 1 cut(s) 1073
BstDEI CTNAG 3 cut(s) 32, 503, 1101
BstF5I GGATG 2 cut(s) 331, 413
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 2 cut(s) 1048, 1372
BstMAI GTCTC 4 cut(s) 169, 919, 1220, 1281
BstMBI GATC 2 cut(s) 1045, 1369
BstMCI CGRYCG 2 cut(s) 303, 439
BstMWI GCNNNNNNNGC 4 cut(s) 55, 212, 656, 695
BstNI CCWGG 1 cut(s) 528
BstPAI GACNNNNGTC 1 cut(s) 299
BstSCI CCNGG 1 cut(s) 526
BstSLI GKGCMC 1 cut(s) 1383
BstV1I GCAGC 2 cut(s) 67, 1007
BstX2I RGATCY 1 cut(s) 1045
BstXI CCANNNNNNTGG 1 cut(s) 614
BstYI RGATCY 1 cut(s) 1045
BstZI CGGCCG 1 cut(s) 436
BsuRI GGCC 1 cut(s) 438
BtgZI GCGATG 1 cut(s) 582
BtsCI GGATG 2 cut(s) 331, 413
BtsIMutI CAGTG 4 cut(s) 42, 249, 582, 1204
Cac8I GCNNGC 1 cut(s) 1073
CaiI CAGNNNCTG 2 cut(s) 564, 803
CfoI GCGC 1 cut(s) 217
Csp6I GTAC 6 cut(s) 98, 107, 562, 602, 959, 1031
CviAII CATG 5 cut(s) 123, 881, 1008, 1052, 1355
CviQI GTAC 6 cut(s) 98, 107, 562, 602, 959, 1031
DdeI CTNAG 3 cut(s) 32, 503, 1101
DpnI GATC 2 cut(s) 1047, 1371
DpnII GATC 2 cut(s) 1045, 1369
EaeI YGGCCR 1 cut(s) 436
EagI CGGCCG 1 cut(s) 436
EclXI CGGCCG 1 cut(s) 436
Eco24I GRGCYC 1 cut(s) 335
Eco31I GGTCTC 1 cut(s) 919
Eco52I CGGCCG 1 cut(s) 436
Eco57I CTGAAG 3 cut(s) 497, 610, 1108
Eco88I CYCGRG 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 498
EcoRII CCWGG 1 cut(s) 526
EcoT38I GRGCYC 1 cut(s) 335
FaeI CATG 5 cut(s) 126, 884, 1011, 1055, 1358
FatI CATG 5 cut(s) 122, 880, 1007, 1051, 1354
FauI CCCGC 1 cut(s) 1105
Fnu4HI GCNGC 2 cut(s) 56, 996
FokI GGATG 2 cut(s) 338, 420
FriOI GRGCYC 1 cut(s) 335
Fsp4HI GCNGC 2 cut(s) 56, 996
FspBI CTAG 1 cut(s) 233
GlaI GCGC 1 cut(s) 216
GluI GCNGC 2 cut(s) 56, 996
GsaI CCCAGC 1 cut(s) 341
GsuI CTGGAG 2 cut(s) 606, 855
HaeIII GGCC 1 cut(s) 438
HhaI GCGC 1 cut(s) 217
Hin1II CATG 5 cut(s) 126, 884, 1011, 1055, 1358
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 1 cut(s) 34
HinfI GANTC 7 cut(s) 15, 226, 279, 549, 967, 1004, 1190
HpaI GTTAAC 1 cut(s) 376
HphI GGTGA 2 cut(s) 212, 1086
Hpy166II GTNNAC 5 cut(s) 100, 376, 391, 604, 1381
Hpy188I TCNGA 7 cut(s) 130, 278, 504, 629, 799, 972, 1362
Hpy188III TCNNGA 4 cut(s) 369, 495, 774, 834
Hpy8I GTNNAC 5 cut(s) 100, 376, 391, 604, 1381
Hpy99I CGWCG 1 cut(s) 69
HpyAV CCTTC 1 cut(s) 192
HpyCH4III ACNGT 6 cut(s) 46, 106, 855, 1025, 1208, 1328
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 212, 656, 695
HpyF3I CTNAG 3 cut(s) 32, 503, 1101
Hsp92II CATG 5 cut(s) 126, 884, 1011, 1055, 1358
HspAI GCGC 1 cut(s) 215
KpnI GGTACC 2 cut(s) 962, 1034
KspAI GTTAAC 1 cut(s) 376
Kzo9I GATC 2 cut(s) 1045, 1369
LmnI GCTCC 6 cut(s) 63, 311, 330, 632, 836, 1363
Lsp1109I GCAGC 2 cut(s) 67, 1007
LweI GCATC 2 cut(s) 193, 790
MaeI CTAG 1 cut(s) 233
MaeIII GTNAC 5 cut(s) 19, 413, 421, 806, 871
MalI GATC 2 cut(s) 1047, 1371
MbiI CCGCTC 1 cut(s) 1100
MboI GATC 2 cut(s) 1045, 1369
MboII GAAGA 6 cut(s) 143, 221, 490, 731, 904, 934
MfeI CAATTG 4 cut(s) 379, 538, 1146, 1269
MflI RGATCY 1 cut(s) 1045
MhlI GDGCHC 4 cut(s) 335, 763, 841, 1383
MlyI GAGTC 1 cut(s) 1184
MmeI TCCRAC 1 cut(s) 256
MseI TTAA 7 cut(s) 291, 375, 522, 816, 1139, 1235, 1257
MspCI CTTAAG 1 cut(s) 290
MspR9I CCNGG 1 cut(s) 528
MunI CAATTG 4 cut(s) 379, 538, 1146, 1269
MvaI CCWGG 1 cut(s) 528
MwoI GCNNNNNNNGC 4 cut(s) 55, 212, 656, 695
NdeII GATC 2 cut(s) 1045, 1369
NlaIII CATG 5 cut(s) 126, 884, 1011, 1055, 1358
NlaIV GGNNCC 7 cut(s) 270, 313, 332, 778, 960, 1032, 1047
NmeAIII GCCGAG 1 cut(s) 414
NmuCI GTSAC 1 cut(s) 421
PfeI GAWTC 6 cut(s) 15, 226, 279, 549, 967, 1004
PkrI GCNGC 2 cut(s) 57, 997
PleI GAGTC 1 cut(s) 1184
PpsI GAGTC 1 cut(s) 1184
PshAI GACNNNNGTC 1 cut(s) 299
PshBI ATTAAT 1 cut(s) 816
Psp6I CCWGG 1 cut(s) 526
PspFI CCCAGC 1 cut(s) 337
PspGI CCWGG 1 cut(s) 526
PspN4I GGNNCC 7 cut(s) 270, 313, 332, 778, 960, 1032, 1047
PstNI CAGNNNCTG 2 cut(s) 564, 803
PsuI RGATCY 1 cut(s) 1045
RsaI GTAC 6 cut(s) 99, 108, 563, 603, 960, 1032
RsaNI GTAC 6 cut(s) 98, 107, 562, 602, 959, 1031
SaqAI TTAA 7 cut(s) 291, 375, 522, 816, 1139, 1235, 1257
SatI GCNGC 2 cut(s) 56, 996
Sau3AI GATC 2 cut(s) 1045, 1369
SchI GAGTC 1 cut(s) 1184
ScrFI CCNGG 1 cut(s) 528
SduI GDGCHC 4 cut(s) 335, 763, 841, 1383
SfaNI GCATC 2 cut(s) 193, 790
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SsiI CCGC 4 cut(s) 299, 1043, 1098, 1167
SspI AATATT 1 cut(s) 981
SspMI CTAG 1 cut(s) 233
StyD4I CCNGG 1 cut(s) 526
TaaI ACNGT 6 cut(s) 46, 106, 855, 1025, 1208, 1328
TaqI TCGA 1 cut(s) 646
TaqII GACCGA 1 cut(s) 1317
TatI WGTACW 3 cut(s) 97, 106, 601
TfiI GAWTC 6 cut(s) 15, 226, 279, 549, 967, 1004
Tru1I TTAA 7 cut(s) 291, 375, 522, 816, 1139, 1235, 1257
Tru9I TTAA 7 cut(s) 291, 375, 522, 816, 1139, 1235, 1257
TscAI CASTG 4 cut(s) 49, 249, 589, 1211
TseFI GTSAC 1 cut(s) 421
TseI GCWGC 2 cut(s) 55, 995
Tsp45I GTSAC 1 cut(s) 421
TspDTI ATGAA 7 cut(s) 17, 143, 174, 450, 731, 996, 1174
TspGWI ACGGA 2 cut(s) 53, 746
TspRI CASTG 4 cut(s) 49, 249, 589, 1211
Vha464I CTTAAG 1 cut(s) 290
VneI GTGCAC 1 cut(s) 1379
VspI ATTAAT 1 cut(s) 816
XapI RAATTY 3 cut(s) 483, 498, 895
XcmI CCANNNNNNNNNTGG 1 cut(s) 345
XspI CTAG 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.