Prupe.5G015500_v2.0.a1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000996
Physical Location & Seq
Forward (+)
1688367 .. 1694882
6516 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G015500.2

Sequence Viewer

Length: 1197 bp
ATGGCCCATGCAAGATTAGGCCAATGGTTAAGTCTTCTAGTATGTTTACTGATATTGCTGAAAGCAGAAAGTCATCCTGTGGGAATCACTCTTGTTGAGAATGCAGTTGCCAAAGGAGCCGTTTGTTTGGATGGGAGCGCACCAGCGTACCACTTTGACAAGGGATTTGGTGCAGGAATCAACAATTGGTTGGTTCATGTTGAGGGAGGAGGATGGTGTAACAGTGTTGAGACTTGCCTTGATCGTAAGAATACTTACCGTGGTTCATCAAACAAAATGGAAAAGTCCATGTCTTTTTCTGGGGTGCTTGGCAACAAGCAAAAGTTTAATCCAGACTTCTATAACTGGAATAGAATCAAAGTTAGGTACTGTGATGGGTCGTCATTCACTGGTGATGTTGAAGCAGTAAACCCAGCAACAAATCTTCACTTCAGAGGAGCAAGAGTTTTTCGCGCCATCATTGATGATCTATTAGCAAAAGGAATGAAAAATGCTCAAAGTGCTCTACTCTCTGGTTGTTCAGCTGGTGGATTGGCCTCAATTCTTAATTGTGATAACTTTCGTTCTCTCCTACCTGTGGGTACTAAAGTAAAATGTCTGGCAGATGCTGGTTACTTCATTAATGCGAAGACTGTTTCTGGATCTCAACAGATTCAAGCCTTTTACAGTGAAGTGGTTGCGCTACATGGTTCGGCAAAGAACTTACCCGCATCCTGCACTTCAAAATTGAGTCCAGGCTTGTGCTTCTTCCCACAAAATGTGGTACCAGTGACCCGGACCCCAATATTTTTGGTAAATGCAGCTTATGATTCATGGCAGATAAAGAACATTTTGGCACCGGGTATTGCTGATCCTAAAGGCGCCTGGAAAAAGTGCAAGCTTGATATAAAGAACTGTTCACCCAGTCAACTTCAAACCATGCAAGAATATCGGTTGCAGTTCTTAGGCGCACTTAGTCATGCCAGCAGCTCCACAACTCATGGACTGTTCATAGACTCTTGCTATGCCCACTGCCAGATTGGAACACAGGAGACATGGTTGGCAGCCGACTCTCCGGTGTTGAGTAAGACGACAATTGGGAAGGCAGTTGGAGACTGGTACACCGACCGAACTCCGTTCCAGAAGATAGATTGTGCTTACCCTTGCAATCCTACATGCAAAAACCGCGTGTTTGATTCGGATGAGCAACAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

399

Amino Acids

43.26

Weight (kDa)

8.67

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 763
AccB1I GGYRCC 3 cut(s) 763, 835, 860
AccII CGCG 2 cut(s) 453, 1167
AciI CCGC 2 cut(s) 708, 1165
AclWI GGATC 2 cut(s) 649, 845
AcuI CTGAAG 1 cut(s) 415
AcyI GRCGYC 1 cut(s) 861
AfaI GTAC 5 cut(s) 149, 368, 583, 765, 1100
AfiI CCNNNNNNNGG 1 cut(s) 577
AgsI TTSAA 4 cut(s) 401, 656, 723, 914
AjnI CCWGG 2 cut(s) 733, 863
AjuI GAANNNNNNNTTGG 2 cut(s) 169, 201
AluBI AGCT 4 cut(s) 524, 803, 880, 969
AluI AGCT 4 cut(s) 524, 803, 880, 969
Alw21I GWGCWC 1 cut(s) 505
Alw26I GTCTC 3 cut(s) 224, 1025, 1086
AlwI GGATC 2 cut(s) 649, 845
AlwNI CAGNNNCTG 1 cut(s) 608
AoxI GGCC 3 cut(s) 3, 19, 534
ApeKI GCWGC 3 cut(s) 800, 966, 1043
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AseI ATTAAT 1 cut(s) 621
Asp718I GGTACC 1 cut(s) 763
AspLEI GCGC 5 cut(s) 140, 455, 682, 863, 950
AspS9I GGNCC 2 cut(s) 4, 777
AsuC2I CCSGG 2 cut(s) 775, 840
AsuHPI GGTGA 2 cut(s) 404, 891
AvaII GGWCC 1 cut(s) 777
BaeI ACNNNNGTAYC 4 cut(s) 131, 164, 573, 606
BanI GGYRCC 3 cut(s) 763, 835, 860
BbsI GAAGAC 2 cut(s) 26, 635
Bbv12I GWGCWC 1 cut(s) 505
BbvI GCAGC 3 cut(s) 812, 978, 1055
BccI CCATC 4 cut(s) 125, 207, 368, 464
BceAI ACGGC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 911, 945
BciT130I CCWGG 2 cut(s) 735, 865
BcnI CCSGG 2 cut(s) 775, 840
BcoDI GTCTC 3 cut(s) 224, 1025, 1086
BfaI CTAG 1 cut(s) 38
BfoI RGCGCY 1 cut(s) 864
BisI GCNGC 3 cut(s) 801, 967, 1044
BlsI GCNGC 3 cut(s) 802, 968, 1045
Bme1390I CCNGG 4 cut(s) 735, 775, 840, 865
Bme18I GGWCC 1 cut(s) 777
BmgT120I GGNCC 2 cut(s) 4, 777
BmiI GGNNCC 5 cut(s) 118, 765, 779, 837, 862
BmrFI CCNGG 4 cut(s) 735, 775, 840, 865
BmrI ACTGGG 1 cut(s) 897
BmsI GCATC 2 cut(s) 595, 719
BmuI ACTGGG 1 cut(s) 897
BpiI GAAGAC 2 cut(s) 26, 635
BpuMI CCSGG 2 cut(s) 775, 840
BsaHI GRCGYC 1 cut(s) 861
BsaJI CCNNGG 1 cut(s) 259
BsaWI WCCGGW 1 cut(s) 1054
BsaXI ACNNNNNCTCC 4 cut(s) 429, 459, 1022, 1052
Bsc4I CCNNNNNNNGG 1 cut(s) 577
Bse1I ACTGG 5 cut(s) 350, 394, 767, 903, 1100
BseBI CCWGG 2 cut(s) 735, 865
BseDI CCNNGG 1 cut(s) 259
BseGI GGATG 5 cut(s) 73, 136, 218, 710, 1186
BseLI CCNNNNNNNGG 1 cut(s) 577
BseNI ACTGG 5 cut(s) 350, 394, 767, 903, 1100
BseRI GAGGAG 2 cut(s) 222, 450
BseXI GCAGC 3 cut(s) 812, 978, 1055
BseYI CCCAGC 1 cut(s) 412
BsgI GTGCAG 2 cut(s) 192, 700
Bsh1236I CGCG 2 cut(s) 453, 1167
Bsh1285I CGRYCG 1 cut(s) 1108
BshFI GGCC 3 cut(s) 5, 21, 536
BshNI GGYRCC 3 cut(s) 763, 835, 860
BsiEI CGRYCG 1 cut(s) 1108
BsiHKAI GWGCWC 1 cut(s) 505
BsiSI CCGG 3 cut(s) 775, 839, 1055
BslI CCNNNNNNNGG 1 cut(s) 577
BsmAI GTCTC 3 cut(s) 224, 1025, 1086
BsmI GAATGC 1 cut(s) 106
BsnI GGCC 3 cut(s) 5, 21, 536
Bsp1286I GDGCHC 1 cut(s) 505
Bsp143I GATC 4 cut(s) 241, 466, 641, 850
BspACI CCGC 2 cut(s) 708, 1165
BspANI GGCC 3 cut(s) 5, 21, 536
BspFNI CGCG 2 cut(s) 453, 1167
BspLI GGNNCC 5 cut(s) 118, 765, 779, 837, 862
BspPI GGATC 2 cut(s) 649, 845
BspT107I GGYRCC 3 cut(s) 763, 835, 860
BsrI ACTGG 5 cut(s) 350, 394, 767, 903, 1100
BssECI CCNNGG 1 cut(s) 259
BssMI GATC 4 cut(s) 241, 466, 641, 850
BssNI GRCGYC 1 cut(s) 861
Bst2UI CCWGG 2 cut(s) 735, 865
Bst4CI ACNGT 7 cut(s) 224, 260, 371, 634, 668, 896, 987
BstACI GRCGYC 1 cut(s) 861
BstC8I GCNNGC 2 cut(s) 878, 964
BstDEI CTNAG 2 cut(s) 943, 953
BstDSI CCRYGG 1 cut(s) 259
BstF5I GGATG 5 cut(s) 73, 136, 218, 710, 1186
BstFNI CGCG 2 cut(s) 453, 1167
BstH2I RGCGCY 1 cut(s) 864
BstHHI GCGC 5 cut(s) 140, 455, 682, 863, 950
BstKTI GATC 4 cut(s) 244, 469, 644, 853
BstMAI GTCTC 3 cut(s) 224, 1025, 1086
BstMBI GATC 4 cut(s) 241, 466, 641, 850
BstMCI CGRYCG 1 cut(s) 1108
BstMWI GCNNNNNNNGC 3 cut(s) 116, 500, 1164
BstNI CCWGG 2 cut(s) 735, 865
BstNSI RCATGY 1 cut(s) 1158
BstSCI CCNGG 4 cut(s) 733, 773, 838, 863
BstUI CGCG 2 cut(s) 453, 1167
BstV1I GCAGC 3 cut(s) 812, 978, 1055
BstV2I GAAGAC 2 cut(s) 26, 635
BstX2I RGATCY 1 cut(s) 641
BstYI RGATCY 1 cut(s) 641
BsuRI GGCC 3 cut(s) 5, 21, 536
BtgI CCRYGG 1 cut(s) 259
BtsCI GGATG 5 cut(s) 73, 136, 218, 710, 1186
BtsI GCAGTG 1 cut(s) 1009
BtsIMutI CAGTG 5 cut(s) 229, 387, 673, 774, 1009
Cac8I GCNNGC 2 cut(s) 878, 964
CaiI CAGNNNCTG 1 cut(s) 608
CfoI GCGC 5 cut(s) 140, 455, 682, 863, 950
Cfr13I GGNCC 2 cut(s) 4, 777
Csp6I GTAC 5 cut(s) 148, 367, 582, 764, 1099
CviQI GTAC 5 cut(s) 148, 367, 582, 764, 1099
DdeI CTNAG 2 cut(s) 943, 953
DinI GGCGCC 1 cut(s) 862
DpnI GATC 4 cut(s) 243, 468, 643, 852
DpnII GATC 4 cut(s) 241, 466, 641, 850
Eco47I GGWCC 1 cut(s) 777
Eco57I CTGAAG 1 cut(s) 415
EcoRII CCWGG 2 cut(s) 733, 863
EgeI GGCGCC 1 cut(s) 862
EheI GGCGCC 1 cut(s) 862
FauI CCCGC 1 cut(s) 715
Fnu4HI GCNGC 3 cut(s) 801, 967, 1044
FokI GGATG 5 cut(s) 60, 143, 225, 697, 1193
Fsp4HI GCNGC 3 cut(s) 801, 967, 1044
FspBI CTAG 1 cut(s) 38
GlaI GCGC 5 cut(s) 139, 454, 681, 862, 949
GluI GCNGC 3 cut(s) 801, 967, 1044
GsaI CCCAGC 1 cut(s) 416
HaeII RGCGCY 1 cut(s) 864
HaeIII GGCC 3 cut(s) 5, 21, 536
HapII CCGG 3 cut(s) 775, 839, 1055
HhaI GCGC 5 cut(s) 140, 455, 682, 863, 950
Hin1I GRCGYC 1 cut(s) 861
Hin6I GCGC 5 cut(s) 138, 453, 680, 861, 948
HinP1I GCGC 5 cut(s) 138, 453, 680, 861, 948
HincII GTYRAC 1 cut(s) 908
HindII GTYRAC 1 cut(s) 908
HindIII AAGCTT 1 cut(s) 878
HinfI GANTC 9 cut(s) 84, 177, 354, 652, 730, 809, 995, 1049, 1175
HpaII CCGG 3 cut(s) 775, 839, 1055
HphI GGTGA 2 cut(s) 404, 891
Hpy166II GTNNAC 5 cut(s) 47, 409, 899, 908, 1101
Hpy188I TCNGA 2 cut(s) 434, 1180
Hpy188III TCNNGA 3 cut(s) 332, 639, 1120
Hpy8I GTNNAC 5 cut(s) 47, 409, 899, 908, 1101
HpyAV CCTTC 1 cut(s) 1075
HpyCH4III ACNGT 7 cut(s) 224, 260, 371, 634, 668, 896, 987
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 500, 1164
HpyF3I CTNAG 2 cut(s) 943, 953
Hsp92I GRCGYC 1 cut(s) 861
HspAI GCGC 5 cut(s) 138, 453, 680, 861, 948
KasI GGCGCC 1 cut(s) 860
KpnI GGTACC 1 cut(s) 767
Kzo9I GATC 4 cut(s) 241, 466, 641, 850
LmnI GCTCC 4 cut(s) 116, 135, 437, 974
Lsp1109I GCAGC 3 cut(s) 812, 978, 1055
LweI GCATC 2 cut(s) 595, 719
MaeI CTAG 1 cut(s) 38
MaeIII GTNAC 3 cut(s) 218, 611, 769
MalI GATC 4 cut(s) 243, 468, 643, 852
MboI GATC 4 cut(s) 241, 466, 641, 850
MboII GAAGA 5 cut(s) 26, 416, 640, 739, 1135
MfeI CAATTG 2 cut(s) 184, 1074
MflI RGATCY 1 cut(s) 641
MhlI GDGCHC 1 cut(s) 505
MluCI AATT 5 cut(s) 184, 540, 547, 725, 1074
Mly113I GGCGCC 1 cut(s) 861
MlyI GAGTC 3 cut(s) 739, 989, 1043
MmeI TCCRAC 1 cut(s) 1069
MnlI CCTC 5 cut(s) 196, 200, 203, 428, 547
MseI TTAA 5 cut(s) 29, 327, 546, 621, 1195
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 3 cut(s) 775, 839, 1055
MspR9I CCNGG 4 cut(s) 735, 775, 840, 865
MunI CAATTG 2 cut(s) 184, 1074
Mva1269I GAATGC 1 cut(s) 106
MvaI CCWGG 2 cut(s) 735, 865
MvnI CGCG 2 cut(s) 453, 1167
MwoI GCNNNNNNNGC 3 cut(s) 116, 500, 1164
NarI GGCGCC 1 cut(s) 861
NciI CCSGG 2 cut(s) 775, 840
NdeII GATC 4 cut(s) 241, 466, 641, 850
NlaIV GGNNCC 5 cut(s) 118, 765, 779, 837, 862
NmuCI GTSAC 1 cut(s) 769
NspI RCATGY 1 cut(s) 1158
PctI GAATGC 1 cut(s) 106
PfeI GAWTC 6 cut(s) 84, 177, 354, 652, 809, 1175
PkrI GCNGC 3 cut(s) 802, 968, 1045
PleI GAGTC 3 cut(s) 738, 989, 1043
PluTI GGCGCC 1 cut(s) 864
PpsI GAGTC 3 cut(s) 738, 989, 1043
PshBI ATTAAT 1 cut(s) 621
Psp6I CCWGG 2 cut(s) 733, 863
PspFI CCCAGC 1 cut(s) 412
PspGI CCWGG 2 cut(s) 733, 863
PspN4I GGNNCC 5 cut(s) 118, 765, 779, 837, 862
PspPI GGNCC 2 cut(s) 4, 777
PstNI CAGNNNCTG 1 cut(s) 608
PsuI RGATCY 1 cut(s) 641
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 5 cut(s) 149, 368, 583, 765, 1100
RsaNI GTAC 5 cut(s) 148, 367, 582, 764, 1099
SaqAI TTAA 5 cut(s) 29, 327, 546, 621, 1195
SatI GCNGC 3 cut(s) 801, 967, 1044
Sau3AI GATC 4 cut(s) 241, 466, 641, 850
Sau96I GGNCC 2 cut(s) 4, 777
SchI GAGTC 3 cut(s) 739, 989, 1043
ScrFI CCNGG 4 cut(s) 735, 775, 840, 865
SduI GDGCHC 1 cut(s) 505
SetI ASST 6 cut(s) 368, 526, 577, 805, 882, 971
SfaNI GCATC 2 cut(s) 595, 719
SfoI GGCGCC 1 cut(s) 862
SinI GGWCC 1 cut(s) 777
Sse9I AATT 5 cut(s) 184, 540, 547, 725, 1074
SsiI CCGC 2 cut(s) 708, 1165
SspDI GGCGCC 1 cut(s) 860
SspI AATATT 1 cut(s) 786
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 4 cut(s) 733, 773, 838, 863
TaaI ACNGT 7 cut(s) 224, 260, 371, 634, 668, 896, 987
TaqII GACCGA 1 cut(s) 1122
TasI AATT 5 cut(s) 184, 540, 547, 725, 1074
TfiI GAWTC 6 cut(s) 84, 177, 354, 652, 809, 1175
Tru1I TTAA 5 cut(s) 29, 327, 546, 621, 1195
Tru9I TTAA 5 cut(s) 29, 327, 546, 621, 1195
TscAI CASTG 5 cut(s) 229, 394, 673, 774, 1016
TseFI GTSAC 1 cut(s) 769
TseI GCWGC 3 cut(s) 800, 966, 1043
Tsp45I GTSAC 1 cut(s) 769
TspDTI ATGAA 6 cut(s) 185, 255, 500, 607, 801, 979
TspGWI ACGGA 1 cut(s) 1104
TspRI CASTG 5 cut(s) 229, 394, 673, 774, 1016
VpaK11BI GGWCC 1 cut(s) 777
VspI ATTAAT 1 cut(s) 621
XceI RCATGY 1 cut(s) 1158
XcmI CCANNNNNNNNNTGG 1 cut(s) 1016
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.