Rmu_ssc0000134.1_g000035

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000134.1
OG0000996
Physical Location & Seq
Reverse (-)
128195 .. 133927
5733 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000134.1_g000035.1.cds

Sequence Viewer

Length: 1404 bp
atggggattcatactcgaaagtcattgagatcatctggctctattgccatgcaggacttaaatgattgcatgatccactcacctcttagtcgtatacttatgattcccagaccaaaacttgaaacttggggcagctccaaccatgggtgccaaacccagttcgagtccccagttgaatttttgcatttagattttctctctcactacactttcagaatggcctatgcaagattaggccagtggttaagtcttctggtatgtttactgatattgctgaaagctgaaagccatcctgtggaactcactttggtccagagtgcagttgccaaaggagctgtttgtttagacgggagcccaccagcataccactttgacaagggatttggtgcagggattaataattggttggttcatattgagggaggaggatggtgtaaggatgttgagagttgcgttgagcgaacgaaaaacttcagaggttcatccacaaaaatggaaaagacaatatctttttctggggttcttggtaaaaagcaaactatgaatccagacttctataactggaacagaatcaaggttaggtactgtgatgggtcatcattcaccggtgatgtggaagcagtagacccggctacaaatcttcacttcagaggaggaagggtttttcgtgccatcattgatgatctattagcaaagggaatgataaatgccaaaaatgctctactctctggttgttcggctggaggattggcttctattcttcaatgtgataacttcggttctctcttacctgccggaactaatgtgaaatgccttgcggatgctggttacttcatcaatgcgaagactattactgggtcacaacaaattgaagctttttatagtcaagtggttgcactacatggttcagcaaagcatttacctgcatcctgcacttcaagattgagaccagggttgtgcttcttcccacaaaatgtgataccgcaactccggactcctattttcttggtaaatgctgcttatgattcgtggcagataaagaacattttggcaccgagtattgctgatcctaaaggcacatggaaaagctgcaagtctggtataaagaactgctcacctgcgcaaattcaaaccatgcaagattataggttacagttcttagatgtactcaatggtggtctcaccaactctccatctcatggagcattcatagactcttgctatgctcactgccaaatcggaactcaagagacatggatggcatctgactccccggttgtcagtaagacgacaattgcaaaggccgtcggagactggtattacaaccgaagtccatcaaaaaagatcgattgtccttacccttgcaatcctacctgcaaaaataaagaatttgattcaaactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

467

Amino Acids

51.25

Weight (kDa)

8.75

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1126
Acc16I TGCGCA 1 cut(s) 1122
Acc36I ACCTGC 4 cut(s) 799, 931, 1126, 1382
AccB1I GGYRCC 2 cut(s) 147, 1051
AccB7I CCANNNNNTGG 2 cut(s) 295, 1199
AccI GTMKAC 2 cut(s) 94, 624
AccIII TCCGGA 1 cut(s) 990
AciI CCGC 2 cut(s) 818, 983
AclWI GGATC 2 cut(s) 67, 1061
AcsI RAATTY 3 cut(s) 176, 1125, 1388
AcuI CTGAAG 2 cut(s) 457, 631
AfaI GTAC 2 cut(s) 584, 1167
AfiI CCNNNNNNNGG 3 cut(s) 144, 295, 1199
AgeI ACCGGT 1 cut(s) 605
AgsI TTSAA 7 cut(s) 122, 176, 764, 872, 939, 1130, 1398
AjnI CCWGG 1 cut(s) 949
AluBI AGCT 5 cut(s) 135, 281, 335, 875, 1089
AluI AGCT 5 cut(s) 135, 281, 335, 875, 1089
Alw26I GTCTC 4 cut(s) 940, 1184, 1244, 1305
AlwI GGATC 2 cut(s) 67, 1061
Aor13HI TCCGGA 1 cut(s) 990
AoxI GGCC 3 cut(s) 219, 235, 1302
ApeKI GCWGC 3 cut(s) 132, 1016, 1089
ApoI RAATTY 3 cut(s) 176, 1125, 1388
ArsI GACNNNNNNTTYG 2 cut(s) 365, 397
AseI ATTAAT 1 cut(s) 396
AsiGI ACCGGT 1 cut(s) 605
Asp700I GAANNNNTTC 1 cut(s) 470
AspLEI GCGC 1 cut(s) 1123
AspS9I GGNCC 1 cut(s) 310
AsuC2I CCSGG 2 cut(s) 629, 1274
AsuHPI GGTGA 5 cut(s) 72, 595, 620, 1107, 1174
AvaII GGWCC 1 cut(s) 310
BanI GGYRCC 2 cut(s) 147, 1051
BanII GRGCYC 1 cut(s) 356
BbsI GAAGAC 2 cut(s) 242, 851
BbvI GCAGC 3 cut(s) 144, 1003, 1076
BccI CCATC 7 cut(s) 297, 423, 584, 680, 1201, 1252, 1342
BceAI ACGGC 1 cut(s) 1289
BciT130I CCWGG 1 cut(s) 951
BcnI CCSGG 2 cut(s) 629, 1274
BcoDI GTCTC 4 cut(s) 940, 1184, 1244, 1305
BfaI CTAG 1 cut(s) 1402
BfuAI ACCTGC 4 cut(s) 799, 931, 1126, 1382
BisI GCNGC 3 cut(s) 133, 1017, 1090
BlsI GCNGC 3 cut(s) 134, 1018, 1091
Bme1390I CCNGG 3 cut(s) 629, 951, 1274
Bme18I GGWCC 1 cut(s) 310
BmgT120I GGNCC 1 cut(s) 310
BmiI GGNNCC 3 cut(s) 149, 353, 1053
BmrFI CCNGG 3 cut(s) 629, 951, 1274
BmrI ACTGGG 3 cut(s) 151, 164, 864
BmsI GCATC 3 cut(s) 811, 935, 1271
BmuI ACTGGG 3 cut(s) 151, 164, 864
BpiI GAAGAC 2 cut(s) 242, 851
BpmI CTGGAG 1 cut(s) 762
BpuEI CTTGAG 1 cut(s) 1230
BpuMI CCSGG 2 cut(s) 629, 1274
Bsa29I ATCGAT 1 cut(s) 1347
BsaI GGTCTC 2 cut(s) 940, 1184
BsaJI CCNNGG 3 cut(s) 142, 950, 1272
BsaWI WCCGGW 2 cut(s) 605, 990
BsaXI ACNNNNNCTCC 6 cut(s) 645, 675, 972, 1002, 1174, 1204
Bsc4I CCNNNNNNNGG 3 cut(s) 144, 295, 1199
Bse118I RCCGGY 1 cut(s) 605
Bse1I ACTGG 6 cut(s) 157, 170, 238, 566, 859, 1319
BseAI TCCGGA 1 cut(s) 990
BseBI CCWGG 1 cut(s) 951
BseCI ATCGAT 1 cut(s) 1347
BseDI CCNNGG 3 cut(s) 142, 950, 1272
BseGI GGATG 7 cut(s) 289, 434, 445, 482, 826, 926, 1263
BseLI CCNNNNNNNGG 3 cut(s) 144, 295, 1199
BseNI ACTGG 6 cut(s) 157, 170, 238, 566, 859, 1319
BseRI GAGGAG 2 cut(s) 438, 666
BseXI GCAGC 3 cut(s) 144, 1003, 1076
BsgI GTGCAG 3 cut(s) 339, 408, 916
BshFI GGCC 3 cut(s) 221, 237, 1304
BshNI GGYRCC 2 cut(s) 147, 1051
BshTI ACCGGT 1 cut(s) 605
BshVI ATCGAT 1 cut(s) 1347
BsiSI CCGG 5 cut(s) 606, 629, 795, 991, 1274
BslFI GGGAC 1 cut(s) 151
BslI CCNNNNNNNGG 3 cut(s) 144, 295, 1199
BsmAI GTCTC 4 cut(s) 940, 1184, 1244, 1305
BsmFI GGGAC 1 cut(s) 151
BsmI GAATGC 1 cut(s) 1205
BsnI GGCC 3 cut(s) 221, 237, 1304
Bso31I GGTCTC 2 cut(s) 940, 1184
Bsp1286I GDGCHC 1 cut(s) 356
Bsp13I TCCGGA 1 cut(s) 990
Bsp143I GATC 5 cut(s) 29, 72, 682, 1066, 1344
Bsp19I CCATGG 1 cut(s) 142
BspACI CCGC 2 cut(s) 818, 983
BspANI GGCC 3 cut(s) 221, 237, 1304
BspDI ATCGAT 1 cut(s) 1347
BspEI TCCGGA 1 cut(s) 990
BspLI GGNNCC 3 cut(s) 149, 353, 1053
BspMI ACCTGC 4 cut(s) 799, 931, 1126, 1382
BspPI GGATC 2 cut(s) 67, 1061
BspT107I GGYRCC 2 cut(s) 147, 1051
BspTNI GGTCTC 2 cut(s) 940, 1184
BsrFI RCCGGY 1 cut(s) 605
BsrI ACTGG 6 cut(s) 157, 170, 238, 566, 859, 1319
BssAI RCCGGY 1 cut(s) 605
BssECI CCNNGG 3 cut(s) 142, 950, 1272
BssMI GATC 5 cut(s) 29, 72, 682, 1066, 1344
BssNAI GTATAC 1 cut(s) 95
BssT1I CCWWGG 1 cut(s) 142
Bst1107I GTATAC 1 cut(s) 95
Bst2UI CCWGG 1 cut(s) 951
Bst4CI ACNGT 2 cut(s) 587, 1155
BstDEI CTNAG 2 cut(s) 86, 1159
BstDSI CCRYGG 1 cut(s) 142
BstF5I GGATG 7 cut(s) 289, 434, 445, 482, 826, 926, 1263
BstHHI GCGC 1 cut(s) 1123
BstKTI GATC 5 cut(s) 32, 75, 685, 1069, 1347
BstMAI GTCTC 4 cut(s) 940, 1184, 1244, 1305
BstMBI GATC 5 cut(s) 29, 72, 682, 1066, 1344
BstMWI GCNNNNNNNGC 2 cut(s) 332, 716
BstNI CCWGG 1 cut(s) 951
BstSCI CCNGG 3 cut(s) 627, 949, 1272
BstV1I GCAGC 3 cut(s) 144, 1003, 1076
BstV2I GAAGAC 2 cut(s) 242, 851
BstXI CCANNNNNNTGG 1 cut(s) 493
BstZ17I GTATAC 1 cut(s) 95
Bsu15I ATCGAT 1 cut(s) 1347
BsuRI GGCC 3 cut(s) 221, 237, 1304
BsuTUI ATCGAT 1 cut(s) 1347
BtgI CCRYGG 1 cut(s) 142
BtsCI GGATG 7 cut(s) 289, 434, 445, 482, 826, 926, 1263
BtsI GCAGTG 1 cut(s) 1228
BtsIMutI CAGTG 2 cut(s) 245, 1228
BveI ACCTGC 4 cut(s) 799, 931, 1126, 1382
CfoI GCGC 1 cut(s) 1123
Cfr10I RCCGGY 1 cut(s) 605
Cfr13I GGNCC 1 cut(s) 310
ClaI ATCGAT 1 cut(s) 1347
Csp6I GTAC 2 cut(s) 583, 1166
CspAI ACCGGT 1 cut(s) 605
CviAII CATG 8 cut(s) 49, 70, 143, 902, 1080, 1135, 1199, 1254
CviQI GTAC 2 cut(s) 583, 1166
DdeI CTNAG 2 cut(s) 86, 1159
DpnI GATC 5 cut(s) 31, 74, 684, 1068, 1346
DpnII GATC 5 cut(s) 29, 72, 682, 1066, 1344
Eco130I CCWWGG 1 cut(s) 142
Eco24I GRGCYC 1 cut(s) 356
Eco31I GGTCTC 2 cut(s) 940, 1184
Eco47I GGWCC 1 cut(s) 310
Eco57I CTGAAG 2 cut(s) 457, 631
EcoRII CCWGG 1 cut(s) 949
EcoT14I CCWWGG 1 cut(s) 142
EcoT38I GRGCYC 1 cut(s) 356
ErhI CCWWGG 1 cut(s) 142
FaeI CATG 8 cut(s) 52, 73, 146, 905, 1083, 1138, 1202, 1257
FaqI GGGAC 1 cut(s) 151
FatI CATG 8 cut(s) 48, 69, 142, 901, 1079, 1134, 1198, 1253
FblI GTMKAC 2 cut(s) 94, 624
Fnu4HI GCNGC 3 cut(s) 133, 1017, 1090
FokI GGATG 7 cut(s) 276, 441, 452, 469, 833, 913, 1270
FriOI GRGCYC 1 cut(s) 356
Fsp4HI GCNGC 3 cut(s) 133, 1017, 1090
FspBI CTAG 1 cut(s) 1402
FspI TGCGCA 1 cut(s) 1122
GlaI GCGC 1 cut(s) 1122
GluI GCNGC 3 cut(s) 133, 1017, 1090
GsuI CTGGAG 1 cut(s) 762
HaeIII GGCC 3 cut(s) 221, 237, 1304
HapII CCGG 5 cut(s) 606, 629, 795, 991, 1274
HhaI GCGC 1 cut(s) 1123
Hin1II CATG 8 cut(s) 52, 73, 146, 905, 1083, 1138, 1202, 1257
Hin6I GCGC 1 cut(s) 1121
HinP1I GCGC 1 cut(s) 1121
HindIII AAGCTT 1 cut(s) 873
HpaII CCGG 5 cut(s) 606, 629, 795, 991, 1274
HphI GGTGA 5 cut(s) 72, 595, 620, 1107, 1174
Hpy166II GTNNAC 3 cut(s) 95, 263, 625
Hpy188I TCNGA 6 cut(s) 215, 476, 650, 1241, 1267, 1310
Hpy188III TCNNGA 5 cut(s) 313, 548, 939, 991, 1247
Hpy8I GTNNAC 3 cut(s) 95, 263, 625
Hpy99I CGWCG 1 cut(s) 1310
HpyAV CCTTC 1 cut(s) 651
HpyCH4III ACNGT 2 cut(s) 587, 1155
HpyF10VI GCNNNNNNNGC 2 cut(s) 332, 716
HpyF3I CTNAG 2 cut(s) 86, 1159
Hsp92II CATG 8 cut(s) 52, 73, 146, 905, 1083, 1138, 1202, 1257
HspAI GCGC 1 cut(s) 1121
Kpn2I TCCGGA 1 cut(s) 990
Kzo9I GATC 5 cut(s) 29, 72, 682, 1066, 1344
LmnI GCTCC 4 cut(s) 140, 332, 351, 1202
Lsp1109I GCAGC 3 cut(s) 144, 1003, 1076
LweI GCATC 3 cut(s) 811, 935, 1271
MaeI CTAG 1 cut(s) 1402
MaeIII GTNAC 3 cut(s) 827, 858, 1149
MalI GATC 5 cut(s) 31, 74, 684, 1068, 1346
MboI GATC 5 cut(s) 29, 72, 682, 1066, 1344
MboII GAAGA 5 cut(s) 242, 632, 752, 856, 955
MfeI CAATTG 1 cut(s) 1293
MhlI GDGCHC 1 cut(s) 356
MluCI AATT 6 cut(s) 176, 400, 867, 1125, 1293, 1388
MlyI GAGTC 4 cut(s) 173, 988, 1208, 1262
MmeI TCCRAC 2 cut(s) 162, 1288
MnlI CCTC 8 cut(s) 93, 412, 416, 419, 470, 644, 647, 737
MroI TCCGGA 1 cut(s) 990
MroXI GAANNNNTTC 1 cut(s) 470
MseI TTAA 3 cut(s) 59, 245, 396
MslI CAYNNNNRTG 1 cut(s) 491
MspI CCGG 5 cut(s) 606, 629, 795, 991, 1274
MspR9I CCNGG 3 cut(s) 629, 951, 1274
MunI CAATTG 1 cut(s) 1293
Mva1269I GAATGC 1 cut(s) 1205
MvaI CCWGG 1 cut(s) 951
MwoI GCNNNNNNNGC 2 cut(s) 332, 716
NciI CCSGG 2 cut(s) 629, 1274
NcoI CCATGG 1 cut(s) 142
NdeII GATC 5 cut(s) 29, 72, 682, 1066, 1344
NlaIII CATG 8 cut(s) 52, 73, 146, 905, 1083, 1138, 1202, 1257
NlaIV GGNNCC 3 cut(s) 149, 353, 1053
NmuCI GTSAC 1 cut(s) 858
NsbI TGCGCA 1 cut(s) 1122
PaqCI CACCTGC 1 cut(s) 1126
PctI GAATGC 1 cut(s) 1205
PdmI GAANNNNTTC 1 cut(s) 470
PfeI GAWTC 6 cut(s) 7, 103, 544, 570, 1025, 1394
PflMI CCANNNNNTGG 2 cut(s) 295, 1199
PinAI ACCGGT 1 cut(s) 605
PkrI GCNGC 3 cut(s) 134, 1018, 1091
PleI GAGTC 4 cut(s) 172, 988, 1208, 1262
PpsI GAGTC 4 cut(s) 172, 988, 1208, 1262
PshBI ATTAAT 1 cut(s) 396
Psp6I CCWGG 1 cut(s) 949
PspGI CCWGG 1 cut(s) 949
PspN4I GGNNCC 3 cut(s) 149, 353, 1053
PspPI GGNCC 1 cut(s) 310
RsaI GTAC 2 cut(s) 584, 1167
RsaNI GTAC 2 cut(s) 583, 1166
RseI CAYNNNNRTG 1 cut(s) 491
SaqAI TTAA 3 cut(s) 59, 245, 396
SatI GCNGC 3 cut(s) 133, 1017, 1090
Sau3AI GATC 5 cut(s) 29, 72, 682, 1066, 1344
Sau96I GGNCC 1 cut(s) 310
SchI GAGTC 4 cut(s) 173, 988, 1208, 1262
ScrFI CCNGG 3 cut(s) 629, 951, 1274
SduI GDGCHC 1 cut(s) 356
SfaNI GCATC 3 cut(s) 811, 935, 1271
SgrAI CRCCGGYG 1 cut(s) 605
SinI GGWCC 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 491
SmlI CTYRAG 1 cut(s) 1245
SmoI CTYRAG 1 cut(s) 1245
Sse9I AATT 6 cut(s) 176, 400, 867, 1125, 1293, 1388
SsiI CCGC 2 cut(s) 818, 983
SspMI CTAG 1 cut(s) 1402
StyD4I CCNGG 3 cut(s) 627, 949, 1272
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 2 cut(s) 587, 1155
TaqI TCGA 3 cut(s) 16, 162, 1347
TasI AATT 6 cut(s) 176, 400, 867, 1125, 1293, 1388
TatI WGTACW 1 cut(s) 1165
TfiI GAWTC 6 cut(s) 7, 103, 544, 570, 1025, 1394
Tru1I TTAA 3 cut(s) 59, 245, 396
Tru9I TTAA 3 cut(s) 59, 245, 396
TscAI CASTG 2 cut(s) 245, 1235
TseFI GTSAC 1 cut(s) 858
TseI GCWGC 3 cut(s) 132, 1016, 1089
Tsp45I GTSAC 1 cut(s) 858
TspDTI ATGAA 5 cut(s) 401, 471, 557, 823, 1198
TspRI CASTG 2 cut(s) 245, 1235
Van91I CCANNNNNTGG 2 cut(s) 295, 1199
VpaK11BI GGWCC 1 cut(s) 310
VspI ATTAAT 1 cut(s) 396
XapI RAATTY 3 cut(s) 176, 1125, 1388
XmiI GTMKAC 2 cut(s) 94, 624
XmnI GAANNNNTTC 1 cut(s) 470
XspI CTAG 1 cut(s) 1402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.