pycom09g14300

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000996
Physical Location & Seq
Reverse (-)
13740062 .. 13742495
2434 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g14300.10

Sequence Viewer

Length: 921 bp
ATGGAAAAGACAATGTCTTTTTCCGGGGTTCTTGGTAACAAGCAACAGACTAATCCAGACTTCTACAATTGGAATAGAATCAAAGTTAGGTACTGTGATGGCTCGTCATTCACTGGTGATGTTGAAGCGGTAGACCCAGCAACAAATCTTCACTTCAGAGGAGCAAGGGTTTTCCGTGCCATCATTGATGATTTATTGGCAAAAGGAATGAAAAACGCTCAAACTGCTCTTATATCTGGTTGTTCGGCTGGCGGCTTGGCCTCGATTCTTCATTGTGATAACTTTCATTCTCTCCTACCTGTGGGTACTAAAGTAAAATGTCTAGCAGATGCTGGTTACTTCATTAATGCGAAGACTGTGTCTGGATCACAACACATTCAAACCTTTTACAGTGAAGTGGTTGCGCTACATGGTTCGGCGAAGAACCTACCTGCATCCTGCACTTCAAGAATGAGTCCAGGGCTGTGCTTCTTCCCACAAAATGTGGTACCCCAAACCCGGACACCAATATTTTTTGTCAATGCAGCTTATGATTCGTGGCAGATAAAGAACATTTTGGCACCGGGTGTTGCTGATCCTAAAGGTGCCTGGAAAAATTGCAAGCTTAGCATAAAGAACTGCTCACCCAGTCAACTTCAAACCATGCAAGAATATAGGTTACAGTTCTTAAACGCACTTGCTGGTGCGAGCAATTCTTCATCTAACGGACTGTTCATCGACTCCTGCTACGCTCATTGCCAAATCGGAACACAGGAGACATGGCTGGCAACTGATTCTCCCATGTTGAGTAAGACGACAATTGCAAAGGCAGTTGGAGACTGGTATTACGACCGCACTCCGTTTAAAAATATTGATTGCCCTTACCCTTGCAATCCCACTTGCAAAAACCGTGAATTTGATTCAAACCATCAGCAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

307

Amino Acids

33.63

Weight (kDa)

8.51

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 439
Acc65I GGTACC 1 cut(s) 487
AccB1I GGYRCC 3 cut(s) 487, 559, 584
AccI GTMKAC 1 cut(s) 132
AciI CCGC 3 cut(s) 128, 252, 832
AclWI GGATC 2 cut(s) 373, 569
AcsI RAATTY 1 cut(s) 893
AcuI CTGAAG 1 cut(s) 139
AdeI CACNNNGTG 1 cut(s) 566
AfaI GTAC 3 cut(s) 92, 307, 489
AfiI CCNNNNNNNGG 2 cut(s) 301, 498
AgsI TTSAA 5 cut(s) 125, 380, 447, 638, 903
AjnI CCWGG 2 cut(s) 457, 587
AluBI AGCT 2 cut(s) 527, 604
AluI AGCT 2 cut(s) 527, 604
Alw26I GTCTC 2 cut(s) 749, 810
AlwI GGATC 2 cut(s) 373, 569
AlwNI CAGNNNCTG 1 cut(s) 332
AoxI GGCC 1 cut(s) 258
ApeKI GCWGC 1 cut(s) 524
ApoI RAATTY 1 cut(s) 893
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
AseI ATTAAT 1 cut(s) 345
Asp718I GGTACC 1 cut(s) 487
AspLEI GCGC 1 cut(s) 406
AsuC2I CCSGG 3 cut(s) 25, 499, 564
AsuHPI GGTGA 2 cut(s) 128, 615
BaeI ACNNNNGTAYC 2 cut(s) 297, 330
BanI GGYRCC 3 cut(s) 487, 559, 584
BbsI GAAGAC 1 cut(s) 359
BbvI GCAGC 1 cut(s) 536
BccI CCATC 3 cut(s) 92, 188, 915
BciT130I CCWGG 2 cut(s) 459, 589
BcnI CCSGG 3 cut(s) 25, 499, 564
BcoDI GTCTC 2 cut(s) 749, 810
BfaI CTAG 1 cut(s) 323
BfuAI ACCTGC 1 cut(s) 439
BisI GCNGC 2 cut(s) 253, 525
BlpI GCTNAGC 1 cut(s) 605
BlsI GCNGC 2 cut(s) 254, 526
Bme1390I CCNGG 5 cut(s) 25, 459, 499, 564, 589
BmiI GGNNCC 3 cut(s) 489, 561, 586
BmrFI CCNGG 5 cut(s) 25, 459, 499, 564, 589
BmrI ACTGGG 1 cut(s) 621
BmsI GCATC 2 cut(s) 319, 443
BmuI ACTGGG 1 cut(s) 621
BpiI GAAGAC 1 cut(s) 359
Bpu1102I GCTNAGC 1 cut(s) 605
BpuMI CCSGG 3 cut(s) 25, 499, 564
BsaJI CCNNGG 2 cut(s) 24, 458
BsaXI ACNNNNNCTCC 4 cut(s) 153, 183, 760, 790
Bsc4I CCNNNNNNNGG 2 cut(s) 301, 498
Bse1I ACTGG 3 cut(s) 118, 627, 824
Bse3DI GCAATG 1 cut(s) 733
BseBI CCWGG 2 cut(s) 459, 589
BseDI CCNNGG 2 cut(s) 24, 458
BseGI GGATG 1 cut(s) 434
BseLI CCNNNNNNNGG 2 cut(s) 301, 498
BseMI GCAATG 1 cut(s) 733
BseNI ACTGG 3 cut(s) 118, 627, 824
BseRI GAGGAG 1 cut(s) 174
BseXI GCAGC 1 cut(s) 536
BseYI CCCAGC 1 cut(s) 136
BsgI GTGCAG 1 cut(s) 424
Bsh1285I CGRYCG 1 cut(s) 832
BshFI GGCC 1 cut(s) 260
BshNI GGYRCC 3 cut(s) 487, 559, 584
BsiEI CGRYCG 1 cut(s) 832
BsiSI CCGG 3 cut(s) 24, 499, 563
BslI CCNNNNNNNGG 2 cut(s) 301, 498
BsmAI GTCTC 2 cut(s) 749, 810
BsnI GGCC 1 cut(s) 260
Bsp143I GATC 2 cut(s) 365, 574
Bsp1720I GCTNAGC 1 cut(s) 605
BspACI CCGC 3 cut(s) 128, 252, 832
BspANI GGCC 1 cut(s) 260
BspLI GGNNCC 3 cut(s) 489, 561, 586
BspMI ACCTGC 1 cut(s) 439
BspPI GGATC 2 cut(s) 373, 569
BspT107I GGYRCC 3 cut(s) 487, 559, 584
BsrDI GCAATG 1 cut(s) 733
BsrI ACTGG 3 cut(s) 118, 627, 824
BssECI CCNNGG 2 cut(s) 24, 458
BssMI GATC 2 cut(s) 365, 574
Bst2UI CCWGG 2 cut(s) 459, 589
Bst4CI ACNGT 6 cut(s) 95, 358, 392, 663, 711, 890
BstC8I GCNNGC 4 cut(s) 250, 602, 688, 765
BstDEI CTNAG 1 cut(s) 605
BstF5I GGATG 1 cut(s) 434
BstHHI GCGC 1 cut(s) 406
BstKTI GATC 2 cut(s) 368, 577
BstMAI GTCTC 2 cut(s) 749, 810
BstMBI GATC 2 cut(s) 365, 574
BstMCI CGRYCG 1 cut(s) 832
BstMWI GCNNNNNNNGC 2 cut(s) 224, 606
BstNI CCWGG 2 cut(s) 459, 589
BstSCI CCNGG 5 cut(s) 23, 457, 497, 562, 587
BstV1I GCAGC 1 cut(s) 536
BstV2I GAAGAC 1 cut(s) 359
BsuRI GGCC 1 cut(s) 260
BtsCI GGATG 1 cut(s) 434
BtsIMutI CAGTG 2 cut(s) 111, 397
BveI ACCTGC 1 cut(s) 439
Cac8I GCNNGC 4 cut(s) 250, 602, 688, 765
CaiI CAGNNNCTG 1 cut(s) 332
CfoI GCGC 1 cut(s) 406
Csp6I GTAC 3 cut(s) 91, 306, 488
CviAII CATG 4 cut(s) 410, 643, 759, 781
CviJI RGCY 8 cut(s) 102, 248, 255, 260, 463, 527, 604, 763
CviKI_1 RGCY 8 cut(s) 102, 248, 255, 260, 463, 527, 604, 763
CviQI GTAC 3 cut(s) 91, 306, 488
DdeI CTNAG 1 cut(s) 605
DpnI GATC 2 cut(s) 367, 576
DpnII GATC 2 cut(s) 365, 574
DraI TTTAAA 1 cut(s) 844
DraIII CACNNNGTG 1 cut(s) 566
Eco57I CTGAAG 1 cut(s) 139
EcoRII CCWGG 2 cut(s) 457, 587
FaeI CATG 4 cut(s) 413, 646, 762, 784
FaiI YATR 8 cut(s) 233, 411, 531, 611, 644, 654, 760, 782
FatI CATG 4 cut(s) 409, 642, 758, 780
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 2 cut(s) 253, 525
FokI GGATG 1 cut(s) 421
Fsp4HI GCNGC 2 cut(s) 253, 525
FspBI CTAG 1 cut(s) 323
GlaI GCGC 1 cut(s) 405
GluI GCNGC 2 cut(s) 253, 525
GsaI CCCAGC 1 cut(s) 140
HaeIII GGCC 1 cut(s) 260
HapII CCGG 3 cut(s) 24, 499, 563
HhaI GCGC 1 cut(s) 406
Hin1II CATG 4 cut(s) 413, 646, 762, 784
Hin6I GCGC 1 cut(s) 404
HinP1I GCGC 1 cut(s) 404
HincII GTYRAC 1 cut(s) 632
HindII GTYRAC 1 cut(s) 632
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 7 cut(s) 78, 265, 454, 533, 719, 773, 899
HpaII CCGG 3 cut(s) 24, 499, 563
HphI GGTGA 2 cut(s) 128, 615
Hpy166II GTNNAC 2 cut(s) 133, 632
Hpy188I TCNGA 2 cut(s) 158, 746
Hpy188III TCNNGA 3 cut(s) 56, 363, 447
Hpy8I GTNNAC 2 cut(s) 133, 632
HpyCH4III ACNGT 6 cut(s) 95, 358, 392, 663, 711, 890
HpyCH4V TGCA 8 cut(s) 434, 441, 524, 600, 646, 803, 870, 882
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 606
HpyF3I CTNAG 1 cut(s) 605
Hsp92II CATG 4 cut(s) 413, 646, 762, 784
HspAI GCGC 1 cut(s) 404
KpnI GGTACC 1 cut(s) 491
Kzo9I GATC 2 cut(s) 365, 574
LmnI GCTCC 1 cut(s) 161
Lsp1109I GCAGC 1 cut(s) 536
LweI GCATC 2 cut(s) 319, 443
MaeI CTAG 1 cut(s) 323
MaeIII GTNAC 3 cut(s) 35, 335, 657
MalI GATC 2 cut(s) 367, 576
MboI GATC 2 cut(s) 365, 574
MboII GAAGA 6 cut(s) 140, 260, 364, 433, 463, 687
MfeI CAATTG 2 cut(s) 67, 798
MluCI AATT 5 cut(s) 67, 595, 691, 798, 893
MlyI GAGTC 2 cut(s) 463, 713
MmeI TCCRAC 1 cut(s) 793
MnlI CCTC 2 cut(s) 152, 271
MseI TTAA 4 cut(s) 345, 668, 843, 919
MspI CCGG 3 cut(s) 24, 499, 563
MspR9I CCNGG 5 cut(s) 25, 459, 499, 564, 589
MunI CAATTG 2 cut(s) 67, 798
MvaI CCWGG 2 cut(s) 459, 589
MwoI GCNNNNNNNGC 2 cut(s) 224, 606
NciI CCSGG 3 cut(s) 25, 499, 564
NdeII GATC 2 cut(s) 365, 574
NlaIII CATG 4 cut(s) 413, 646, 762, 784
NlaIV GGNNCC 3 cut(s) 489, 561, 586
PfeI GAWTC 5 cut(s) 78, 265, 533, 773, 899
PflFI GACNNNGTC 2 cut(s) 13, 358
PkrI GCNGC 2 cut(s) 254, 526
PleI GAGTC 2 cut(s) 462, 713
PpsI GAGTC 2 cut(s) 462, 713
PshBI ATTAAT 1 cut(s) 345
Psp6I CCWGG 2 cut(s) 457, 587
PspFI CCCAGC 1 cut(s) 136
PspGI CCWGG 2 cut(s) 457, 587
PspN4I GGNNCC 3 cut(s) 489, 561, 586
PstNI CAGNNNCTG 1 cut(s) 332
PsyI GACNNNGTC 2 cut(s) 13, 358
RsaI GTAC 3 cut(s) 92, 307, 489
RsaNI GTAC 3 cut(s) 91, 306, 488
SaqAI TTAA 4 cut(s) 345, 668, 843, 919
SatI GCNGC 2 cut(s) 253, 525
Sau3AI GATC 2 cut(s) 365, 574
SchI GAGTC 2 cut(s) 463, 713
ScrFI CCNGG 5 cut(s) 25, 459, 499, 564, 589
SetI ASST 9 cut(s) 92, 301, 386, 429, 433, 529, 586, 606, 659
SfaNI GCATC 2 cut(s) 319, 443
Sse9I AATT 5 cut(s) 67, 595, 691, 798, 893
SsiI CCGC 3 cut(s) 128, 252, 832
SspI AATATT 2 cut(s) 510, 850
SspMI CTAG 1 cut(s) 323
StyD4I CCNGG 5 cut(s) 23, 457, 497, 562, 587
TaaI ACNGT 6 cut(s) 95, 358, 392, 663, 711, 890
TaqI TCGA 2 cut(s) 263, 717
TasI AATT 5 cut(s) 67, 595, 691, 798, 893
TauI GCSGC 1 cut(s) 255
TfiI GAWTC 5 cut(s) 78, 265, 533, 773, 899
Tru1I TTAA 4 cut(s) 345, 668, 843, 919
Tru9I TTAA 4 cut(s) 345, 668, 843, 919
TscAI CASTG 2 cut(s) 118, 397
TseI GCWGC 1 cut(s) 524
TspDTI ATGAA 6 cut(s) 224, 260, 275, 331, 687, 703
TspGWI ACGGA 3 cut(s) 164, 720, 828
TspRI CASTG 2 cut(s) 118, 397
Tth111I GACNNNGTC 2 cut(s) 13, 358
VspI ATTAAT 1 cut(s) 345
XapI RAATTY 1 cut(s) 893
XmiI GTMKAC 1 cut(s) 132
XspI CTAG 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.