Rh7BG277700

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000996
Physical Location & Seq
Reverse (-)
25798713 .. 25803161
4449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG277700.1

Sequence Viewer

Length: 750 bp
ATGGGTGATGCAAGATTAGCGCAGTGGTTGAATCTTCTAGTATGTTCAGTGATAATGATGAAAGCAACGGGAGTTCCTATCGGAATCACTATCCTTAAGACTGCGGTCGCCAGAGGAGCTGTTTGTTTGGATGGAAGCCCCCCAGCTTACCATTGGGATAAGGGGTTTGGTTCAGGAGTTAACAATTGGTTGGTTCACATTGAGGGAGGAGGATGGTGTAACAATGTCACAAATTGCCTTGGCCGTAAGAAAACTCGTTTAGGTTCATCAAAGAAAATGCTGAAGGAAATTCCTTTTTCTGGAATTCTAAGCAACAGGCAATCACTTAATCCAGGCTTTTACAATTGGAATAGAATCAAAGTCAGGTACTGTGATGGGTCATCATTCACTGGAGATGTTGAAGCAGTACACCCAGCTACTGGTCTTTACTTCAGAGGAGCTAGGGTTTTTCGAGCCGTTATTGCTGATTTATTAGCAAAAGGAATGGGGAAAGCTGAAAATGCTGTCCTCTCCGGTTGTTCTGCTGGTGGATTGGCCTCCATTCTTCATTGTGATAACTTCCGTGCTCTCTTACCTTCCGGAACCATTGTGAAATGTGTTTCAGATGCTGGTTACTTTATTAATGTAAAGGACATTTCTGGAGCACAACACATTCAACAGTATTTTGCTGATGTTGTTACAACACATGTAATCCTCTCGCTCCCTCCTGCATACAATGAAGACTTTACCGCCATCAATCCCCATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

249

Amino Acids

26.82

Weight (kDa)

9.13

Isoelectric Point (pI)

30.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 23 - 233 8.5e-103 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 419
AccIII TCCGGA 1 cut(s) 578
AciI CCGC 2 cut(s) 104, 729
AcoI YGGCCR 1 cut(s) 241
AcsI RAATTY 2 cut(s) 288, 303
AcuI CTGAAG 2 cut(s) 302, 415
AfaI GTAC 2 cut(s) 368, 408
AfiI CCNNNNNNNGG 2 cut(s) 299, 419
AflII CTTAAG 1 cut(s) 95
AflIII ACRYGT 1 cut(s) 685
AgsI TTSAA 3 cut(s) 31, 401, 656
AjnI CCWGG 1 cut(s) 331
AluBI AGCT 5 cut(s) 119, 146, 416, 440, 494
AluI AGCT 5 cut(s) 119, 146, 416, 440, 494
Alw21I GWGCWC 2 cut(s) 568, 646
AlwNI CAGNNNCTG 3 cut(s) 369, 419, 608
Aor13HI TCCGGA 1 cut(s) 578
AoxI GGCC 2 cut(s) 241, 534
ApoI RAATTY 2 cut(s) 288, 303
AseI ATTAAT 1 cut(s) 621
AspLEI GCGC 1 cut(s) 22
AsuHPI GGTGA 1 cut(s) 17
BbsI GAAGAC 1 cut(s) 726
Bbv12I GWGCWC 2 cut(s) 568, 646
BccI CCATC 4 cut(s) 125, 207, 368, 740
BceAI ACGGC 2 cut(s) 228, 440
BciT130I CCWGG 1 cut(s) 333
BfaI CTAG 2 cut(s) 38, 441
BfrI CTTAAG 1 cut(s) 95
Bme1390I CCNGG 1 cut(s) 333
BmiI GGNNCC 1 cut(s) 583
BmrFI CCNGG 1 cut(s) 333
BmsI GCATC 1 cut(s) 595
BoxI GACNNNNGTC 1 cut(s) 104
BpiI GAAGAC 1 cut(s) 726
BpmI CTGGAG 2 cut(s) 411, 660
BsaJI CCNNGG 1 cut(s) 238
BsaWI WCCGGW 2 cut(s) 512, 578
BsaXI ACNNNNNCTCC 2 cut(s) 429, 459
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 419
Bse1I ACTGG 2 cut(s) 394, 424
BseAI TCCGGA 1 cut(s) 578
BseBI CCWGG 1 cut(s) 333
BseDI CCNNGG 1 cut(s) 238
BseGI GGATG 2 cut(s) 136, 218
BseLI CCNNNNNNNGG 2 cut(s) 299, 419
BseNI ACTGG 2 cut(s) 394, 424
BseRI GAGGAG 3 cut(s) 129, 222, 450
BseYI CCCAGC 2 cut(s) 142, 412
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 2 cut(s) 243, 536
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 2 cut(s) 568, 646
BsiSI CCGG 2 cut(s) 513, 579
BslI CCNNNNNNNGG 2 cut(s) 299, 419
BsnI GGCC 2 cut(s) 243, 536
Bsp1286I GDGCHC 2 cut(s) 568, 646
Bsp13I TCCGGA 1 cut(s) 578
BspACI CCGC 2 cut(s) 104, 729
BspANI GGCC 2 cut(s) 243, 536
BspEI TCCGGA 1 cut(s) 578
BspLI GGNNCC 1 cut(s) 583
BspTI CTTAAG 1 cut(s) 95
BsrI ACTGG 2 cut(s) 394, 424
BssECI CCNNGG 1 cut(s) 238
BssT1I CCWWGG 1 cut(s) 238
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 2 cut(s) 371, 660
BstAFI CTTAAG 1 cut(s) 95
BstDEI CTNAG 1 cut(s) 308
BstF5I GGATG 2 cut(s) 136, 218
BstHHI GCGC 1 cut(s) 22
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 4 cut(s) 17, 116, 461, 500
BstNI CCWGG 1 cut(s) 333
BstNSI RCATGY 1 cut(s) 689
BstPAI GACNNNNGTC 1 cut(s) 104
BstSCI CCNGG 1 cut(s) 331
BstV2I GAAGAC 1 cut(s) 726
BsuRI GGCC 2 cut(s) 243, 536
BtsCI GGATG 2 cut(s) 136, 218
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 3 cut(s) 29, 54, 387
CaiI CAGNNNCTG 3 cut(s) 369, 419, 608
CfoI GCGC 1 cut(s) 22
Csp6I GTAC 2 cut(s) 367, 407
CviAII CATG 1 cut(s) 686
CviQI GTAC 2 cut(s) 367, 407
DdeI CTNAG 1 cut(s) 308
EaeI YGGCCR 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 238
Eco57I CTGAAG 2 cut(s) 302, 415
EcoRI GAATTC 1 cut(s) 303
EcoRII CCWGG 1 cut(s) 331
EcoT14I CCWWGG 1 cut(s) 238
ErhI CCWWGG 1 cut(s) 238
FaeI CATG 1 cut(s) 689
FaiI YATR 3 cut(s) 43, 687, 712
FatI CATG 1 cut(s) 685
FokI GGATG 2 cut(s) 143, 225
FspBI CTAG 2 cut(s) 38, 441
GlaI GCGC 1 cut(s) 21
GsaI CCCAGC 2 cut(s) 146, 416
GsuI CTGGAG 2 cut(s) 411, 660
HaeIII GGCC 2 cut(s) 243, 536
HapII CCGG 2 cut(s) 513, 579
HhaI GCGC 1 cut(s) 22
Hin1II CATG 1 cut(s) 689
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 3 cut(s) 31, 84, 354
HpaI GTTAAC 1 cut(s) 181
HpaII CCGG 2 cut(s) 513, 579
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 3 cut(s) 181, 196, 409
Hpy188I TCNGA 4 cut(s) 83, 434, 604, 749
Hpy188III TCNNGA 4 cut(s) 174, 300, 579, 639
Hpy8I GTNNAC 3 cut(s) 181, 196, 409
HpyAV CCTTC 2 cut(s) 277, 585
HpyCH4III ACNGT 2 cut(s) 371, 660
HpyCH4V TGCA 2 cut(s) 11, 710
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 116, 461, 500
HpyF3I CTNAG 1 cut(s) 308
Hsp92II CATG 1 cut(s) 689
HspAI GCGC 1 cut(s) 20
Kpn2I TCCGGA 1 cut(s) 578
KspAI GTTAAC 1 cut(s) 181
LmnI GCTCC 4 cut(s) 116, 437, 641, 705
LweI GCATC 1 cut(s) 595
MaeI CTAG 2 cut(s) 38, 441
MaeIII GTNAC 4 cut(s) 218, 226, 611, 676
MboII GAAGA 3 cut(s) 26, 536, 731
MfeI CAATTG 2 cut(s) 184, 343
MhlI GDGCHC 2 cut(s) 568, 646
MluCI AATT 5 cut(s) 184, 232, 288, 303, 343
MnlI CCTC 9 cut(s) 107, 196, 200, 203, 428, 518, 547, 704, 714
MroI TCCGGA 1 cut(s) 578
MseI TTAA 4 cut(s) 96, 180, 327, 621
MspCI CTTAAG 1 cut(s) 95
MspI CCGG 2 cut(s) 513, 579
MspR9I CCNGG 1 cut(s) 333
MunI CAATTG 2 cut(s) 184, 343
MvaI CCWGG 1 cut(s) 333
MwoI GCNNNNNNNGC 4 cut(s) 17, 116, 461, 500
NlaIII CATG 1 cut(s) 689
NlaIV GGNNCC 1 cut(s) 583
NmuCI GTSAC 1 cut(s) 226
NspI RCATGY 1 cut(s) 689
PciI ACATGT 1 cut(s) 685
PfeI GAWTC 3 cut(s) 31, 84, 354
PflMI CCANNNNNTGG 1 cut(s) 419
PscI ACATGT 1 cut(s) 685
PshAI GACNNNNGTC 1 cut(s) 104
PshBI ATTAAT 1 cut(s) 621
Psp6I CCWGG 1 cut(s) 331
PspFI CCCAGC 2 cut(s) 142, 412
PspGI CCWGG 1 cut(s) 331
PspN4I GGNNCC 1 cut(s) 583
PstNI CAGNNNCTG 3 cut(s) 369, 419, 608
RsaI GTAC 2 cut(s) 368, 408
RsaNI GTAC 2 cut(s) 367, 407
SaqAI TTAA 4 cut(s) 96, 180, 327, 621
ScrFI CCNGG 1 cut(s) 333
SduI GDGCHC 2 cut(s) 568, 646
SetI ASST 8 cut(s) 121, 148, 265, 368, 418, 442, 496, 577
SfaNI GCATC 1 cut(s) 595
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 5 cut(s) 184, 232, 288, 303, 343
SsiI CCGC 2 cut(s) 104, 729
SspMI CTAG 2 cut(s) 38, 441
StyD4I CCNGG 1 cut(s) 331
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 2 cut(s) 371, 660
TaqI TCGA 1 cut(s) 451
TasI AATT 5 cut(s) 184, 232, 288, 303, 343
TatI WGTACW 1 cut(s) 406
TfiI GAWTC 3 cut(s) 31, 84, 354
Tru1I TTAA 4 cut(s) 96, 180, 327, 621
Tru9I TTAA 4 cut(s) 96, 180, 327, 621
TscAI CASTG 3 cut(s) 29, 54, 394
TseFI GTSAC 1 cut(s) 226
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 4 cut(s) 74, 255, 536, 732
TspGWI ACGGA 1 cut(s) 551
TspRI CASTG 3 cut(s) 29, 54, 394
Van91I CCANNNNNTGG 1 cut(s) 419
Vha464I CTTAAG 1 cut(s) 95
VspI ATTAAT 1 cut(s) 621
XapI RAATTY 2 cut(s) 288, 303
XceI RCATGY 1 cut(s) 689
XcmI CCANNNNNNNNNTGG 1 cut(s) 150
XspI CTAG 2 cut(s) 38, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.