RchiOBHm_Chr7g0214981

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000996
Physical Location & Seq
Reverse (-)
32397982 .. 32402459
4478 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19235

Sequence Viewer

Length: 1143 bp
ATGCTGAAAGCAACGGGAGTTCCTATCGGAATCACTATCCTTAAGACTGCGGTCGCCAGAGGAGCTGTTTGTTTGGATGGAAGCCCCCCAGCTTACCATTGGGATAAGGGGTTTGGTTCAGGAGTTAACAATTGGTTGGTTCACATTGAGGGAGGAGGATGGTGTCACAATGTCACAAATTGCCTTGGCCGTACGAACACTCGTTTAGGTTCATCAAAGAAAATGCAGAAGGAAATTCCTTTTTCTGGAATTCTAAGCAACAGGCAATCACTTAATCCAGGCTTTTACAATTGGAATAGAATCAAAGTCAGGTACTGTGATGGGTCATCATTCACTGGAGATGTTGAAGCAGTACACCCAGCTACTGGTCTTTACTTTAGAGGAGCTAGGGTTTTTCGAGCCGTTATTGCTGATTTATTAGCAAAAGGAATGGGGAAAGCTGAAAATGCTGTCCTCTCCGGTTGTTCTGCTGGTGGATTGGCCTCCATTCTTCATTGTGATAACTTCCGTGCTCTCTTACCTTCCGGAACCATTGTGAAATGTGTTTCAGATGCTGGTTACTTTATTAATGTAAAGGACATTTCTGGAGCACAACACATTCAACAGTATTTTGCTGATGTTGTTACTACACATGGTTCTGCCAAGAATTTGCCTCCATCTTGCACTTCAAAATTGAGACCAGATTTGTGTTTCTTCCCTCAATATGTGGTACCACAGATTCGGACACCAATATTCTATGTACATTCAGCCTATGATTCATGGCAGATAAGGAACATTTTGGTACCAGGTGTTGCGGATCCTCATGGAACTTGGCGAAACTGCAAGCTCAATATAAAGAACTGCTCACCCGCTCAGCTCAAAACCATTGAAAGCTTCAGGTTGCAATTTTTAACTGCAATTGGTGGTGTGAGCAACTCTGCAGTAAAGGGAATGTTCATAGACTCTTGCTATCTACACTGTCAAACTGAAATGCAGGAGACTTGGTTAATGCCTAATTCTCCACTCCTTAACAAAATGACAATTGCAGGGGCAGTTAGAGACTGGTTTTATGACCGAACTCCATTCCAAAAGATAGACTGTCCTTACTCTTGCAATCCCACTTGCCATCCTCCGATGTTTGATCCAAATGTGCACTTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

380

Amino Acids

41.78

Weight (kDa)

8.95

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 5 - 369 1.1e-172 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 709, 781
AccB1I GGYRCC 2 cut(s) 709, 781
AccB7I CCANNNNNTGG 1 cut(s) 365
AccBSI CCGCTC 1 cut(s) 851
AccIII TCCGGA 1 cut(s) 524
AciI CCGC 3 cut(s) 50, 794, 849
AclWI GGATC 3 cut(s) 791, 804, 1115
AcoI YGGCCR 1 cut(s) 187
AcsI RAATTY 3 cut(s) 234, 249, 646
AcuI CTGAAG 1 cut(s) 859
AfaI GTAC 6 cut(s) 193, 314, 354, 711, 741, 783
AfiI CCNNNNNNNGG 2 cut(s) 245, 365
AflII CTTAAG 1 cut(s) 41
AgsI TTSAA 4 cut(s) 347, 602, 669, 869
AjnI CCWGG 2 cut(s) 277, 784
AluBI AGCT 8 cut(s) 65, 92, 362, 386, 440, 826, 856, 873
AluI AGCT 8 cut(s) 65, 92, 362, 386, 440, 826, 856, 873
Alw21I GWGCWC 3 cut(s) 514, 592, 1134
Alw26I GTCTC 3 cut(s) 670, 971, 1032
Alw44I GTGCAC 1 cut(s) 1130
AlwI GGATC 3 cut(s) 791, 804, 1115
AlwNI CAGNNNCTG 3 cut(s) 315, 365, 554
Aor13HI TCCGGA 1 cut(s) 524
AoxI GGCC 2 cut(s) 187, 480
ApaLI GTGCAC 1 cut(s) 1130
ApoI RAATTY 3 cut(s) 234, 249, 646
AseI ATTAAT 1 cut(s) 567
Asp718I GGTACC 2 cut(s) 709, 781
AsuHPI GGTGA 1 cut(s) 837
BaeGI GKGCMC 1 cut(s) 1134
BamHI GGATCC 1 cut(s) 796
BanI GGYRCC 2 cut(s) 709, 781
Bbv12I GWGCWC 3 cut(s) 514, 592, 1134
BccI CCATC 5 cut(s) 71, 153, 314, 664, 1113
BceAI ACGGC 2 cut(s) 174, 386
BciT130I CCWGG 2 cut(s) 279, 786
BcoDI GTCTC 3 cut(s) 670, 971, 1032
BfaI CTAG 1 cut(s) 387
BfmI CTRYAG 1 cut(s) 918
BfrI CTTAAG 1 cut(s) 41
BlpI GCTNAGC 1 cut(s) 852
Bme1390I CCNGG 2 cut(s) 279, 786
BmiI GGNNCC 4 cut(s) 529, 711, 783, 798
BmrFI CCNGG 2 cut(s) 279, 786
BmsI GCATC 1 cut(s) 541
BoxI GACNNNNGTC 1 cut(s) 50
BpmI CTGGAG 2 cut(s) 357, 606
Bpu1102I GCTNAGC 1 cut(s) 852
BsaI GGTCTC 1 cut(s) 670
BsaJI CCNNGG 1 cut(s) 184
BsaWI WCCGGW 2 cut(s) 458, 524
BsaXI ACNNNNNCTCC 4 cut(s) 375, 405, 968, 998
Bsc4I CCNNNNNNNGG 2 cut(s) 245, 365
Bse1I ACTGG 3 cut(s) 340, 370, 1046
BseAI TCCGGA 1 cut(s) 524
BseBI CCWGG 2 cut(s) 279, 786
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 3 cut(s) 82, 164, 1105
BseLI CCNNNNNNNGG 2 cut(s) 245, 365
BseMII CTCAG 1 cut(s) 866
BseNI ACTGG 3 cut(s) 340, 370, 1046
BseRI GAGGAG 3 cut(s) 75, 168, 396
BseSI GKGCMC 1 cut(s) 1134
BseYI CCCAGC 2 cut(s) 88, 358
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 2 cut(s) 189, 482
BshNI GGYRCC 2 cut(s) 709, 781
BsiEI CGRYCG 1 cut(s) 54
BsiHKAI GWGCWC 3 cut(s) 514, 592, 1134
BsiSI CCGG 2 cut(s) 459, 525
BsiWI CGTACG 1 cut(s) 191
BslI CCNNNNNNNGG 2 cut(s) 245, 365
BsmAI GTCTC 3 cut(s) 670, 971, 1032
BsnI GGCC 2 cut(s) 189, 482
Bso31I GGTCTC 1 cut(s) 670
Bsp1286I GDGCHC 3 cut(s) 514, 592, 1134
Bsp13I TCCGGA 1 cut(s) 524
Bsp1407I TGTACA 1 cut(s) 739
Bsp143I GATC 2 cut(s) 796, 1120
Bsp1720I GCTNAGC 1 cut(s) 852
BspACI CCGC 3 cut(s) 50, 794, 849
BspANI GGCC 2 cut(s) 189, 482
BspCNI CTCAG 1 cut(s) 865
BspEI TCCGGA 1 cut(s) 524
BspLI GGNNCC 4 cut(s) 529, 711, 783, 798
BspMAI CTGCAG 1 cut(s) 922
BspPI GGATC 3 cut(s) 791, 804, 1115
BspT107I GGYRCC 2 cut(s) 709, 781
BspTI CTTAAG 1 cut(s) 41
BspTNI GGTCTC 1 cut(s) 670
BsrBI CCGCTC 1 cut(s) 851
BsrGI TGTACA 1 cut(s) 739
BsrI ACTGG 3 cut(s) 340, 370, 1046
BssECI CCNNGG 1 cut(s) 184
BssMI GATC 2 cut(s) 796, 1120
BssT1I CCWWGG 1 cut(s) 184
Bst2UI CCWGG 2 cut(s) 279, 786
Bst4CI ACNGT 4 cut(s) 317, 606, 959, 1079
BstAFI CTTAAG 1 cut(s) 41
BstAUI TGTACA 1 cut(s) 739
BstC8I GCNNGC 1 cut(s) 824
BstDEI CTNAG 2 cut(s) 254, 852
BstF5I GGATG 3 cut(s) 82, 164, 1105
BstKTI GATC 2 cut(s) 799, 1123
BstMAI GTCTC 3 cut(s) 670, 971, 1032
BstMBI GATC 2 cut(s) 796, 1120
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 3 cut(s) 62, 407, 446
BstNI CCWGG 2 cut(s) 279, 786
BstPAI GACNNNNGTC 1 cut(s) 50
BstSCI CCNGG 2 cut(s) 277, 784
BstSFI CTRYAG 1 cut(s) 918
BstSLI GKGCMC 1 cut(s) 1134
BstX2I RGATCY 1 cut(s) 796
BstYI RGATCY 1 cut(s) 796
BsuRI GGCC 2 cut(s) 189, 482
BtsCI GGATG 3 cut(s) 82, 164, 1105
BtsIMutI CAGTG 2 cut(s) 333, 955
Cac8I GCNNGC 1 cut(s) 824
CaiI CAGNNNCTG 3 cut(s) 315, 365, 554
CsiI ACCWGGT 1 cut(s) 784
Csp6I GTAC 6 cut(s) 192, 313, 353, 710, 740, 782
CviAII CATG 3 cut(s) 632, 759, 803
CviQI GTAC 6 cut(s) 192, 313, 353, 710, 740, 782
DdeI CTNAG 2 cut(s) 254, 852
DpnI GATC 2 cut(s) 798, 1122
DpnII GATC 2 cut(s) 796, 1120
EaeI YGGCCR 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 184
Eco31I GGTCTC 1 cut(s) 670
Eco57I CTGAAG 1 cut(s) 859
EcoRI GAATTC 1 cut(s) 249
EcoRII CCWGG 2 cut(s) 277, 784
EcoT14I CCWWGG 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 184
FaeI CATG 3 cut(s) 635, 762, 806
FaiI YATR 9 cut(s) 633, 705, 738, 753, 760, 804, 833, 938, 1050
FatI CATG 3 cut(s) 631, 758, 802
FauI CCCGC 1 cut(s) 856
FokI GGATG 3 cut(s) 89, 171, 1092
FspBI CTAG 1 cut(s) 387
GsaI CCCAGC 2 cut(s) 92, 362
GsuI CTGGAG 2 cut(s) 357, 606
HaeIII GGCC 2 cut(s) 189, 482
HapII CCGG 2 cut(s) 459, 525
Hin1II CATG 3 cut(s) 635, 762, 806
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 871
HinfI GANTC 5 cut(s) 30, 300, 718, 755, 941
HpaI GTTAAC 1 cut(s) 127
HpaII CCGG 2 cut(s) 459, 525
HphI GGTGA 1 cut(s) 837
Hpy166II GTNNAC 4 cut(s) 127, 142, 355, 1132
Hpy188I TCNGA 4 cut(s) 29, 550, 723, 1113
Hpy188III TCNNGA 4 cut(s) 120, 246, 525, 585
Hpy8I GTNNAC 4 cut(s) 127, 142, 355, 1132
HpyAV CCTTC 2 cut(s) 223, 531
HpyCH4III ACNGT 4 cut(s) 317, 606, 959, 1079
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 407, 446
HpyF3I CTNAG 2 cut(s) 254, 852
Hsp92II CATG 3 cut(s) 635, 762, 806
Kpn2I TCCGGA 1 cut(s) 524
KpnI GGTACC 2 cut(s) 713, 785
KspAI GTTAAC 1 cut(s) 127
Kzo9I GATC 2 cut(s) 796, 1120
LmnI GCTCC 3 cut(s) 62, 383, 587
LweI GCATC 1 cut(s) 541
MabI ACCWGGT 1 cut(s) 784
MaeI CTAG 1 cut(s) 387
MaeIII GTNAC 4 cut(s) 164, 172, 557, 622
MalI GATC 2 cut(s) 798, 1122
MbiI CCGCTC 1 cut(s) 851
MboI GATC 2 cut(s) 796, 1120
MboII GAAGA 2 cut(s) 482, 685
MfeI CAATTG 4 cut(s) 130, 289, 897, 1020
MflI RGATCY 1 cut(s) 796
MhlI GDGCHC 3 cut(s) 514, 592, 1134
MlyI GAGTC 1 cut(s) 935
MroI TCCGGA 1 cut(s) 524
MseI TTAA 7 cut(s) 42, 126, 273, 567, 890, 986, 1008
MspCI CTTAAG 1 cut(s) 41
MspI CCGG 2 cut(s) 459, 525
MspR9I CCNGG 2 cut(s) 279, 786
MunI CAATTG 4 cut(s) 130, 289, 897, 1020
MvaI CCWGG 2 cut(s) 279, 786
MwoI GCNNNNNNNGC 3 cut(s) 62, 407, 446
NdeII GATC 2 cut(s) 796, 1120
NlaIII CATG 3 cut(s) 635, 762, 806
NlaIV GGNNCC 4 cut(s) 529, 711, 783, 798
NmuCI GTSAC 2 cut(s) 164, 172
PfeI GAWTC 4 cut(s) 30, 300, 718, 755
Pfl23II CGTACG 1 cut(s) 191
PflMI CCANNNNNTGG 1 cut(s) 365
PleI GAGTC 1 cut(s) 935
PpsI GAGTC 1 cut(s) 935
PshAI GACNNNNGTC 1 cut(s) 50
PshBI ATTAAT 1 cut(s) 567
Psp6I CCWGG 2 cut(s) 277, 784
PspFI CCCAGC 2 cut(s) 88, 358
PspGI CCWGG 2 cut(s) 277, 784
PspLI CGTACG 1 cut(s) 191
PspN4I GGNNCC 4 cut(s) 529, 711, 783, 798
PstI CTGCAG 1 cut(s) 922
PstNI CAGNNNCTG 3 cut(s) 315, 365, 554
PsuI RGATCY 1 cut(s) 796
RsaI GTAC 6 cut(s) 193, 314, 354, 711, 741, 783
RsaNI GTAC 6 cut(s) 192, 313, 353, 710, 740, 782
SaqAI TTAA 7 cut(s) 42, 126, 273, 567, 890, 986, 1008
Sau3AI GATC 2 cut(s) 796, 1120
SchI GAGTC 1 cut(s) 935
ScrFI CCNGG 2 cut(s) 279, 786
SduI GDGCHC 3 cut(s) 514, 592, 1134
SexAI ACCWGGT 1 cut(s) 784
SfaNI GCATC 1 cut(s) 541
SfcI CTRYAG 1 cut(s) 918
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
SsiI CCGC 3 cut(s) 50, 794, 849
SspI AATATT 1 cut(s) 732
SspMI CTAG 1 cut(s) 387
StyD4I CCNGG 2 cut(s) 277, 784
StyI CCWWGG 1 cut(s) 184
TaaI ACNGT 4 cut(s) 317, 606, 959, 1079
TaqI TCGA 1 cut(s) 397
TaqII GACCGA 1 cut(s) 1068
TatI WGTACW 2 cut(s) 352, 739
TfiI GAWTC 4 cut(s) 30, 300, 718, 755
Tru1I TTAA 7 cut(s) 42, 126, 273, 567, 890, 986, 1008
Tru9I TTAA 7 cut(s) 42, 126, 273, 567, 890, 986, 1008
TscAI CASTG 2 cut(s) 340, 962
TseFI GTSAC 2 cut(s) 164, 172
Tsp45I GTSAC 2 cut(s) 164, 172
TspDTI ATGAA 4 cut(s) 201, 482, 747, 925
TspGWI ACGGA 1 cut(s) 497
TspRI CASTG 2 cut(s) 340, 962
Van91I CCANNNNNTGG 1 cut(s) 365
Vha464I CTTAAG 1 cut(s) 41
VneI GTGCAC 1 cut(s) 1130
VspI ATTAAT 1 cut(s) 567
XapI RAATTY 3 cut(s) 234, 249, 646
XcmI CCANNNNNNNNNTGG 1 cut(s) 96
XspI CTAG 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.