pycom08g17750

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000996
Physical Location & Seq
Reverse (-)
17892640 .. 17895291
2652 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g17750.1

Sequence Viewer

Length: 1179 bp
ATGGCCTGTTCAAGATTAGGCCAATGGTTAACTCTTCTAGTATGTTTACTGATATTGCTGAAAGCAGAAAGCCACCTTGTGGGAATCACTCTTGTCGAGAATGCAGTTGCTAACGGAGCCGTTTGTTTGGATGGGAGCGCACCCGGTTACCACTTTGACAAGGGATTTGGTGCCGGAATCAACAATTGGTTGGTTTATTTTGAGGGTGGAGGATGGTGTAACACCGTTGCAACTTGCCTTGATCGCTCGAATAATTCCCTCGGTTCATCAACCAAAATGGAAAAGACAGTGTCGTTTTCTGCGGTTCTTAGTAACAAGCAAAAGACTAATCCAGACTTCTACAATTGGAATAGAATCAAAGTTAGATACTGTGATGGCTCGTCATTTACTGGTGATGTTGAAGCGGTAGACCCTGCAACAAATCTTCACTTCAGAGGAGCAAGGGTTTTCCGTGCCATCATGGATGATTTATTGGCAAAAGGAATGAAAAATGCTCAATCTGCTCTTCTATCTGGTTGTTCGGCTGGCGGATTGGCCTCGATTCTTCATTGTGATAACTTCCGTTCTCTTCTACCTGTGGATATTAAAGTGAAATGTGTACCAGATGCTGGTTACTTCATTAATGCGAGGACGGTGTCTGGATCACCATACATTCAAGCCTTTTATAGTGAAGTGGTTGCGCTACATGGTTCGGTGAAGAACTTGCCTGCATCCTGCACTTCAAGAATGAGTCCAGGGCTGTGCTTCTTCCCACAAAATGTGGTACCACTAACCCGGACGCCAATATTTTTCGTCAATGCAGCTTACGATTGGTGGCAGATACAGAACATTTTGGCACCGGATGTTGCTGATCCTGAAGGTGCCTGGAAAAAGTGCAAGCTTAATATAAAGAACTGCTCACCCAGTCAGCTTGAAACCATGCAAGAATTTAGGTTACAGTTCTTAAAAACGATTGCTGGTGCGAGCAATTCTTCATCTCATGGGTGGTTCATACACTCTTGCTATTCCCATTGCCAAATTGAATTCCAGGAGACATGGCTTGCAGCTGACTCTCCTATGTTGAGTAAGACGACAATTGCAGAGGCAGTTGGAGACTGGTATTACGACCTCACTCCGTTCAGAAAGATCGATTGCCCTTACCCTTGTAATCCCACTTGCAAAAACGCTGAATTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

393

Amino Acids

43.13

Weight (kDa)

7.43

Isoelectric Point (pI)

40.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 21 - 387 1.9e-173 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 763
AccB1I GGYRCC 4 cut(s) 170, 763, 835, 860
AccB7I CCANNNNNTGG 2 cut(s) 79, 608
AccI GTMKAC 1 cut(s) 408
AciI CCGC 3 cut(s) 302, 404, 528
AclWI GGATC 2 cut(s) 649, 845
AcsI RAATTY 3 cut(s) 926, 1022, 1169
AcuI CTGAAG 2 cut(s) 415, 876
AcyI GRCGYC 1 cut(s) 779
AdeI CACNNNGTG 1 cut(s) 79
AfaI GTAC 2 cut(s) 600, 765
AfiI CCNNNNNNNGG 2 cut(s) 79, 608
AgsI TTSAA 6 cut(s) 12, 401, 656, 723, 914, 1022
AjnI CCWGG 3 cut(s) 733, 863, 1026
AjuI GAANNNNNNNTTGG 2 cut(s) 169, 201
AluBI AGCT 4 cut(s) 803, 880, 910, 1046
AluI AGCT 4 cut(s) 803, 880, 910, 1046
Alw26I GTCTC 2 cut(s) 1025, 1086
AlwI GGATC 2 cut(s) 649, 845
AlwNI CAGNNNCTG 1 cut(s) 608
AoxI GGCC 3 cut(s) 3, 19, 534
ApeKI GCWGC 2 cut(s) 800, 1043
ApoI RAATTY 3 cut(s) 926, 1022, 1169
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AseI ATTAAT 1 cut(s) 621
Asp718I GGTACC 1 cut(s) 763
AspLEI GCGC 2 cut(s) 140, 682
AsuC2I CCSGG 2 cut(s) 144, 775
AsuHPI GGTGA 4 cut(s) 404, 636, 706, 891
BanI GGYRCC 4 cut(s) 170, 763, 835, 860
BbvI GCAGC 2 cut(s) 812, 1055
BccI CCATC 4 cut(s) 125, 207, 368, 464
BceAI ACGGC 1 cut(s) 104
BciT130I CCWGG 3 cut(s) 735, 865, 1028
BcnI CCSGG 2 cut(s) 144, 775
BcoDI GTCTC 2 cut(s) 1025, 1086
BfaI CTAG 1 cut(s) 38
BisI GCNGC 2 cut(s) 801, 1044
BlsI GCNGC 2 cut(s) 802, 1045
Bme1390I CCNGG 5 cut(s) 144, 735, 775, 865, 1028
BmiI GGNNCC 5 cut(s) 118, 172, 765, 837, 862
BmrFI CCNGG 5 cut(s) 144, 735, 775, 865, 1028
BmrI ACTGGG 1 cut(s) 897
BmsI GCATC 2 cut(s) 595, 719
BmuI ACTGGG 1 cut(s) 897
BpuMI CCSGG 2 cut(s) 144, 775
Bsa29I ATCGAT 1 cut(s) 1128
BsaHI GRCGYC 1 cut(s) 779
BsaJI CCNNGG 2 cut(s) 259, 734
BsaWI WCCGGW 1 cut(s) 838
BsaXI ACNNNNNCTCC 2 cut(s) 429, 459
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 608
Bse1I ACTGG 3 cut(s) 394, 903, 1100
Bse3DI GCAATG 1 cut(s) 1009
BseBI CCWGG 3 cut(s) 735, 865, 1028
BseCI ATCGAT 1 cut(s) 1128
BseDI CCNNGG 2 cut(s) 259, 734
BseGI GGATG 5 cut(s) 136, 218, 469, 710, 847
BseLI CCNNNNNNNGG 2 cut(s) 79, 608
BseMI GCAATG 1 cut(s) 1009
BseNI ACTGG 3 cut(s) 394, 903, 1100
BseRI GAGGAG 1 cut(s) 450
BseXI GCAGC 2 cut(s) 812, 1055
BsgI GTGCAG 1 cut(s) 700
BshFI GGCC 3 cut(s) 5, 21, 536
BshNI GGYRCC 4 cut(s) 170, 763, 835, 860
BshVI ATCGAT 1 cut(s) 1128
BsiSI CCGG 4 cut(s) 144, 174, 775, 839
BslI CCNNNNNNNGG 2 cut(s) 79, 608
BsmAI GTCTC 2 cut(s) 1025, 1086
BsmI GAATGC 1 cut(s) 106
BsnI GGCC 3 cut(s) 5, 21, 536
Bsp143I GATC 4 cut(s) 241, 641, 850, 1125
BspACI CCGC 3 cut(s) 302, 404, 528
BspANI GGCC 3 cut(s) 5, 21, 536
BspDI ATCGAT 1 cut(s) 1128
BspLI GGNNCC 5 cut(s) 118, 172, 765, 837, 862
BspPI GGATC 2 cut(s) 649, 845
BspQI GCTCTTC 1 cut(s) 510
BspT107I GGYRCC 4 cut(s) 170, 763, 835, 860
BsrDI GCAATG 1 cut(s) 1009
BsrI ACTGG 3 cut(s) 394, 903, 1100
BssECI CCNNGG 2 cut(s) 259, 734
BssMI GATC 4 cut(s) 241, 641, 850, 1125
BssNI GRCGYC 1 cut(s) 779
Bst2UI CCWGG 3 cut(s) 735, 865, 1028
Bst4CI ACNGT 5 cut(s) 226, 289, 371, 634, 939
Bst6I CTCTTC 3 cut(s) 39, 510, 573
BstACI GRCGYC 1 cut(s) 779
BstC8I GCNNGC 5 cut(s) 526, 708, 878, 964, 1041
BstDEI CTNAG 1 cut(s) 308
BstEII GGTNACC 1 cut(s) 146
BstF5I GGATG 5 cut(s) 136, 218, 469, 710, 847
BstHHI GCGC 2 cut(s) 140, 682
BstKTI GATC 4 cut(s) 244, 644, 853, 1128
BstMAI GTCTC 2 cut(s) 1025, 1086
BstMBI GATC 4 cut(s) 241, 641, 850, 1125
BstMWI GCNNNNNNNGC 3 cut(s) 116, 243, 500
BstNI CCWGG 3 cut(s) 735, 865, 1028
BstPI GGTNACC 1 cut(s) 146
BstSCI CCNGG 5 cut(s) 142, 733, 773, 863, 1026
BstV1I GCAGC 2 cut(s) 812, 1055
Bsu15I ATCGAT 1 cut(s) 1128
BsuRI GGCC 3 cut(s) 5, 21, 536
BsuTUI ATCGAT 1 cut(s) 1128
BtsCI GGATG 5 cut(s) 136, 218, 469, 710, 847
BtsIMutI CAGTG 1 cut(s) 294
Cac8I GCNNGC 5 cut(s) 526, 708, 878, 964, 1041
CaiI CAGNNNCTG 1 cut(s) 608
CfoI GCGC 2 cut(s) 140, 682
ClaI ATCGAT 1 cut(s) 1128
CseI GACGC 1 cut(s) 787
Csp6I GTAC 2 cut(s) 599, 764
CviAII CATG 5 cut(s) 460, 686, 919, 980, 1035
CviQI GTAC 2 cut(s) 599, 764
DdeI CTNAG 1 cut(s) 308
DpnI GATC 4 cut(s) 243, 643, 852, 1127
DpnII GATC 4 cut(s) 241, 641, 850, 1125
DraIII CACNNNGTG 1 cut(s) 79
Eam1104I CTCTTC 3 cut(s) 39, 510, 573
EarI CTCTTC 3 cut(s) 39, 510, 573
EciI GGCGGA 1 cut(s) 543
Eco57I CTGAAG 2 cut(s) 415, 876
Eco91I GGTNACC 1 cut(s) 146
EcoO65I GGTNACC 1 cut(s) 146
EcoRI GAATTC 1 cut(s) 1022
EcoRII CCWGG 3 cut(s) 733, 863, 1026
FaeI CATG 5 cut(s) 463, 689, 922, 983, 1038
FatI CATG 5 cut(s) 459, 685, 918, 979, 1034
FblI GTMKAC 1 cut(s) 408
Fnu4HI GCNGC 2 cut(s) 801, 1044
FokI GGATG 5 cut(s) 143, 225, 476, 697, 854
Fsp4HI GCNGC 2 cut(s) 801, 1044
FspBI CTAG 1 cut(s) 38
GlaI GCGC 2 cut(s) 139, 681
GluI GCNGC 2 cut(s) 801, 1044
HaeIII GGCC 3 cut(s) 5, 21, 536
HapII CCGG 4 cut(s) 144, 174, 775, 839
HgaI GACGC 1 cut(s) 787
HhaI GCGC 2 cut(s) 140, 682
Hin1I GRCGYC 1 cut(s) 779
Hin1II CATG 5 cut(s) 463, 689, 922, 983, 1038
Hin6I GCGC 2 cut(s) 138, 680
HinP1I GCGC 2 cut(s) 138, 680
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HindIII AAGCTT 1 cut(s) 878
HinfI GANTC 6 cut(s) 84, 177, 354, 541, 730, 1049
HpaI GTTAAC 1 cut(s) 30
HpaII CCGG 4 cut(s) 144, 174, 775, 839
HphI GGTGA 4 cut(s) 404, 636, 706, 891
Hpy166II GTNNAC 4 cut(s) 30, 47, 409, 599
Hpy188I TCNGA 2 cut(s) 434, 1121
Hpy188III TCNNGA 6 cut(s) 12, 97, 332, 639, 723, 854
Hpy8I GTNNAC 4 cut(s) 30, 47, 409, 599
HpyAV CCTTC 1 cut(s) 851
HpyCH4III ACNGT 5 cut(s) 226, 289, 371, 634, 939
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 243, 500
HpyF3I CTNAG 1 cut(s) 308
Hsp92I GRCGYC 1 cut(s) 779
Hsp92II CATG 5 cut(s) 463, 689, 922, 983, 1038
HspAI GCGC 2 cut(s) 138, 680
KpnI GGTACC 1 cut(s) 767
KspAI GTTAAC 1 cut(s) 30
Kzo9I GATC 4 cut(s) 241, 641, 850, 1125
LguI GCTCTTC 1 cut(s) 510
LmnI GCTCC 3 cut(s) 116, 135, 437
Lsp1109I GCAGC 2 cut(s) 812, 1055
LweI GCATC 2 cut(s) 595, 719
MaeI CTAG 1 cut(s) 38
MaeIII GTNAC 5 cut(s) 146, 218, 311, 611, 933
MalI GATC 4 cut(s) 243, 643, 852, 1127
MboI GATC 4 cut(s) 241, 641, 850, 1125
MboII GAAGA 8 cut(s) 26, 416, 497, 536, 560, 709, 739, 963
MfeI CAATTG 3 cut(s) 184, 343, 1074
MluCI AATT 9 cut(s) 184, 253, 343, 926, 967, 1017, 1022, 1074, 1169
MlyI GAGTC 2 cut(s) 739, 1043
MmeI TCCRAC 1 cut(s) 1069
MnlI CCTC 8 cut(s) 196, 203, 269, 428, 547, 621, 1075, 1118
MseI TTAA 6 cut(s) 29, 585, 621, 882, 944, 1177
MspA1I CMGCKG 1 cut(s) 1046
MspI CCGG 4 cut(s) 144, 174, 775, 839
MspR9I CCNGG 5 cut(s) 144, 735, 775, 865, 1028
MunI CAATTG 3 cut(s) 184, 343, 1074
Mva1269I GAATGC 1 cut(s) 106
MvaI CCWGG 3 cut(s) 735, 865, 1028
MwoI GCNNNNNNNGC 3 cut(s) 116, 243, 500
NciI CCSGG 2 cut(s) 144, 775
NdeII GATC 4 cut(s) 241, 641, 850, 1125
NlaIII CATG 5 cut(s) 463, 689, 922, 983, 1038
NlaIV GGNNCC 5 cut(s) 118, 172, 765, 837, 862
PciSI GCTCTTC 1 cut(s) 510
PctI GAATGC 1 cut(s) 106
PfeI GAWTC 4 cut(s) 84, 177, 354, 541
PflFI GACNNNGTC 2 cut(s) 289, 634
PflMI CCANNNNNTGG 2 cut(s) 79, 608
PfoI TCCNGGA 1 cut(s) 1026
PkrI GCNGC 2 cut(s) 802, 1045
PleI GAGTC 2 cut(s) 738, 1043
PpsI GAGTC 2 cut(s) 738, 1043
PshBI ATTAAT 1 cut(s) 621
Psp6I CCWGG 3 cut(s) 733, 863, 1026
PspEI GGTNACC 1 cut(s) 146
PspGI CCWGG 3 cut(s) 733, 863, 1026
PspN4I GGNNCC 5 cut(s) 118, 172, 765, 837, 862
PstNI CAGNNNCTG 1 cut(s) 608
PsyI GACNNNGTC 2 cut(s) 289, 634
PvuII CAGCTG 1 cut(s) 1046
RsaI GTAC 2 cut(s) 600, 765
RsaNI GTAC 2 cut(s) 599, 764
SapI GCTCTTC 1 cut(s) 510
SaqAI TTAA 6 cut(s) 29, 585, 621, 882, 944, 1177
SatI GCNGC 2 cut(s) 801, 1044
Sau3AI GATC 4 cut(s) 241, 641, 850, 1125
SchI GAGTC 2 cut(s) 739, 1043
ScrFI CCNGG 5 cut(s) 144, 735, 775, 865, 1028
SetI ASST 9 cut(s) 78, 577, 805, 862, 882, 912, 935, 1048, 1110
SfaNI GCATC 2 cut(s) 595, 719
Sse9I AATT 9 cut(s) 184, 253, 343, 926, 967, 1017, 1022, 1074, 1169
SsiI CCGC 3 cut(s) 302, 404, 528
SspI AATATT 1 cut(s) 786
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 5 cut(s) 142, 733, 773, 863, 1026
TaaI ACNGT 5 cut(s) 226, 289, 371, 634, 939
TaqI TCGA 4 cut(s) 96, 248, 539, 1128
TasI AATT 9 cut(s) 184, 253, 343, 926, 967, 1017, 1022, 1074, 1169
TfiI GAWTC 4 cut(s) 84, 177, 354, 541
Tru1I TTAA 6 cut(s) 29, 585, 621, 882, 944, 1177
Tru9I TTAA 6 cut(s) 29, 585, 621, 882, 944, 1177
TscAI CASTG 1 cut(s) 294
TseI GCWGC 2 cut(s) 800, 1043
TspDTI ATGAA 6 cut(s) 255, 500, 536, 607, 963, 979
TspGWI ACGGA 4 cut(s) 129, 440, 551, 1104
TspRI CASTG 1 cut(s) 294
Tth111I GACNNNGTC 2 cut(s) 289, 634
Van91I CCANNNNNTGG 2 cut(s) 79, 608
VspI ATTAAT 1 cut(s) 621
XapI RAATTY 3 cut(s) 926, 1022, 1169
XmiI GTMKAC 1 cut(s) 408
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.