Rh7BG277500

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000996
Physical Location & Seq
Reverse (-)
25789264 .. 25790870
1607 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG277500.1

Sequence Viewer

Length: 837 bp
ATGTTTAATACTTATATTTGCAGAATGGCCTATGCAAGATTAGGCCAGTGGTTAAGTCTTCTGGTATGTTTACTGATATTGATAAAAGCTGAAAGCCATCCTGTGGAACTCACTTTGGTCCAGAGTGCAGTTGCCAAAGGAGCTGTTTGTTTAGACGGGAGCCCACCGGCATACCACTTTGACAAGGGATTTGGTGCAGGGATTAACAATTGGTTGGTTCATATTGAGGGAGGAGGATGGTGTAAGGATGTTGAGAGTTGCGTTGAGCGAACAAAAAACTTCAGAGGTTCATCCAAAAAAATGGAAAAGACAATATCTTTTTCTGGGATTCTTGGTAAAAAGCAAACTATGAATCCAGACTTCTATAACTGGAACAGAATCAAGGTTAGGTACTGTGATGGGTCATCATTCACCGGTGATGTGGAAGCAGTAGACCCAGCTACAAATCTTCACTTCAGAGGAGGAAGGGTTTTTCGTGCCATCATTGAGGATCTATTAGCAAAGGGAATGATAAATGCCAAAAATGCTCTACTCTCTGGTTGTTCGGCTGGAGGATTGGCTTCTATTCTTCAATGTGATAACTTCCGTTCTCTCTTACCCGCCGGAACTAATGTGAAATGCCTTGCGGATGCTGGTTACTTCATCAATGGGAAGACTATTACTGGATCACAACAAATTGAAGCCTTTTATAGTCAAGTGGTTGCACTACATGTAATATATCTCTGTCAGGCCTTTAGTATGTGCATGTGGTACCATTGGCTTTGTGTGGTTCTGATTGCATATGTTTTGTGCAGGGTTCAGCAAAGCATTTACCTGCATCCTGCACTTCAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

278

Amino Acids

30.75

Weight (kDa)

8.64

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 29 - 239 1.4e-104 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 822
Acc65I GGTACC 1 cut(s) 750
AccB1I GGYRCC 1 cut(s) 750
AccB7I CCANNNNNTGG 1 cut(s) 103
AccI GTMKAC 1 cut(s) 432
AciI CCGC 2 cut(s) 600, 626
AclWI GGATC 2 cut(s) 498, 673
AcuI CTGAAG 2 cut(s) 265, 439
AfaI GTAC 2 cut(s) 392, 752
AfiI CCNNNNNNNGG 1 cut(s) 103
AflIII ACRYGT 1 cut(s) 709
AgeI ACCGGT 1 cut(s) 413
AgsI TTSAA 3 cut(s) 572, 680, 830
AluBI AGCT 3 cut(s) 89, 143, 440
AluI AGCT 3 cut(s) 89, 143, 440
AlwI GGATC 2 cut(s) 498, 673
AoxI GGCC 3 cut(s) 27, 43, 729
ArsI GACNNNNNNTTYG 2 cut(s) 173, 205
AsiGI ACCGGT 1 cut(s) 413
Asp718I GGTACC 1 cut(s) 750
AspS9I GGNCC 1 cut(s) 118
AsuHPI GGTGA 2 cut(s) 403, 428
AvaII GGWCC 1 cut(s) 118
BanI GGYRCC 1 cut(s) 750
BanII GRGCYC 1 cut(s) 164
BbsI GAAGAC 2 cut(s) 50, 659
BccI CCATC 4 cut(s) 105, 231, 392, 488
BfuAI ACCTGC 1 cut(s) 822
Bme18I GGWCC 1 cut(s) 118
BmgT120I GGNCC 1 cut(s) 118
BmiI GGNNCC 2 cut(s) 161, 752
BmsI GCATC 2 cut(s) 619, 826
BpiI GAAGAC 2 cut(s) 50, 659
BpmI CTGGAG 1 cut(s) 570
BsaWI WCCGGW 1 cut(s) 413
BsaXI ACNNNNNCTCC 2 cut(s) 453, 483
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse118I RCCGGY 2 cut(s) 166, 413
Bse1I ACTGG 3 cut(s) 46, 374, 667
BseGI GGATG 6 cut(s) 97, 242, 253, 290, 634, 817
BseLI CCNNNNNNNGG 1 cut(s) 103
BseNI ACTGG 3 cut(s) 46, 374, 667
BseRI GAGGAG 2 cut(s) 246, 474
BseYI CCCAGC 1 cut(s) 436
BsgI GTGCAG 4 cut(s) 147, 216, 807, 811
BshFI GGCC 3 cut(s) 29, 45, 731
BshNI GGYRCC 1 cut(s) 750
BshTI ACCGGT 1 cut(s) 413
BsiSI CCGG 3 cut(s) 167, 414, 603
BslI CCNNNNNNNGG 1 cut(s) 103
BsnI GGCC 3 cut(s) 29, 45, 731
Bsp1286I GDGCHC 1 cut(s) 164
Bsp143I GATC 2 cut(s) 490, 665
BspACI CCGC 2 cut(s) 600, 626
BspANI GGCC 3 cut(s) 29, 45, 731
BspLI GGNNCC 2 cut(s) 161, 752
BspMI ACCTGC 1 cut(s) 822
BspPI GGATC 2 cut(s) 498, 673
BspT107I GGYRCC 1 cut(s) 750
BsrFI RCCGGY 2 cut(s) 166, 413
BsrI ACTGG 3 cut(s) 46, 374, 667
BssAI RCCGGY 2 cut(s) 166, 413
BssMI GATC 2 cut(s) 490, 665
Bst4CI ACNGT 1 cut(s) 395
BstF5I GGATG 6 cut(s) 97, 242, 253, 290, 634, 817
BstKTI GATC 2 cut(s) 493, 668
BstMBI GATC 2 cut(s) 490, 665
BstMWI GCNNNNNNNGC 2 cut(s) 140, 524
BstNSI RCATGY 2 cut(s) 713, 748
BstV2I GAAGAC 2 cut(s) 50, 659
BstX2I RGATCY 1 cut(s) 490
BstXI CCANNNNNNTGG 1 cut(s) 301
BstYI RGATCY 1 cut(s) 490
BsuRI GGCC 3 cut(s) 29, 45, 731
BtsCI GGATG 6 cut(s) 97, 242, 253, 290, 634, 817
BtsIMutI CAGTG 1 cut(s) 53
BveI ACCTGC 1 cut(s) 822
Cfr10I RCCGGY 2 cut(s) 166, 413
Cfr13I GGNCC 1 cut(s) 118
Csp6I GTAC 2 cut(s) 391, 751
CspAI ACCGGT 1 cut(s) 413
CviAII CATG 2 cut(s) 710, 745
CviQI GTAC 2 cut(s) 391, 751
DpnI GATC 2 cut(s) 492, 667
DpnII GATC 2 cut(s) 490, 665
Eco147I AGGCCT 1 cut(s) 731
Eco24I GRGCYC 1 cut(s) 164
Eco47I GGWCC 1 cut(s) 118
Eco57I CTGAAG 2 cut(s) 265, 439
EcoT38I GRGCYC 1 cut(s) 164
FaeI CATG 2 cut(s) 713, 748
FatI CATG 2 cut(s) 709, 744
FauI CCCGC 1 cut(s) 607
FauNDI CATATG 1 cut(s) 781
FblI GTMKAC 1 cut(s) 432
FokI GGATG 6 cut(s) 84, 249, 260, 277, 641, 804
FriOI GRGCYC 1 cut(s) 164
GsaI CCCAGC 1 cut(s) 440
GsuI CTGGAG 1 cut(s) 570
HaeIII GGCC 3 cut(s) 29, 45, 731
HapII CCGG 3 cut(s) 167, 414, 603
Hin1II CATG 2 cut(s) 713, 748
HinfI GANTC 3 cut(s) 328, 352, 378
HpaII CCGG 3 cut(s) 167, 414, 603
HphI GGTGA 2 cut(s) 403, 428
Hpy166II GTNNAC 2 cut(s) 71, 433
Hpy188I TCNGA 3 cut(s) 284, 458, 774
Hpy188III TCNNGA 3 cut(s) 121, 356, 830
Hpy8I GTNNAC 2 cut(s) 71, 433
HpyAV CCTTC 1 cut(s) 459
HpyCH4III ACNGT 1 cut(s) 395
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 524
Hsp92II CATG 2 cut(s) 713, 748
KpnI GGTACC 1 cut(s) 754
Kzo9I GATC 2 cut(s) 490, 665
LmnI GCTCC 2 cut(s) 140, 159
LweI GCATC 2 cut(s) 619, 826
MaeIII GTNAC 1 cut(s) 635
MalI GATC 2 cut(s) 492, 667
MboI GATC 2 cut(s) 490, 665
MboII GAAGA 4 cut(s) 50, 440, 560, 664
MfeI CAATTG 1 cut(s) 208
MflI RGATCY 1 cut(s) 490
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 2 cut(s) 208, 675
MnlI CCTC 8 cut(s) 220, 224, 227, 278, 452, 455, 481, 545
MseI TTAA 3 cut(s) 6, 53, 204
MspI CCGG 3 cut(s) 167, 414, 603
MunI CAATTG 1 cut(s) 208
MwoI GCNNNNNNNGC 2 cut(s) 140, 524
NdeI CATATG 1 cut(s) 781
NdeII GATC 2 cut(s) 490, 665
NlaIII CATG 2 cut(s) 713, 748
NlaIV GGNNCC 2 cut(s) 161, 752
NspI RCATGY 2 cut(s) 713, 748
PceI AGGCCT 1 cut(s) 731
PciI ACATGT 1 cut(s) 709
PfeI GAWTC 3 cut(s) 328, 352, 378
PflMI CCANNNNNTGG 1 cut(s) 103
PinAI ACCGGT 1 cut(s) 413
PscI ACATGT 1 cut(s) 709
PspFI CCCAGC 1 cut(s) 436
PspN4I GGNNCC 2 cut(s) 161, 752
PspPI GGNCC 1 cut(s) 118
PsuI RGATCY 1 cut(s) 490
RsaI GTAC 2 cut(s) 392, 752
RsaNI GTAC 2 cut(s) 391, 751
SaqAI TTAA 3 cut(s) 6, 53, 204
Sau3AI GATC 2 cut(s) 490, 665
Sau96I GGNCC 1 cut(s) 118
SduI GDGCHC 1 cut(s) 164
SetI ASST 7 cut(s) 91, 145, 289, 387, 392, 442, 816
SfaNI GCATC 2 cut(s) 619, 826
SgrAI CRCCGGYG 1 cut(s) 413
SinI GGWCC 1 cut(s) 118
Sse9I AATT 2 cut(s) 208, 675
SseBI AGGCCT 1 cut(s) 731
SsiI CCGC 2 cut(s) 600, 626
StuI AGGCCT 1 cut(s) 731
TaaI ACNGT 1 cut(s) 395
TasI AATT 2 cut(s) 208, 675
TfiI GAWTC 3 cut(s) 328, 352, 378
Tru1I TTAA 3 cut(s) 6, 53, 204
Tru9I TTAA 3 cut(s) 6, 53, 204
TscAI CASTG 1 cut(s) 53
TspDTI ATGAA 4 cut(s) 209, 279, 365, 631
TspGWI ACGGA 1 cut(s) 575
TspRI CASTG 1 cut(s) 53
Van91I CCANNNNNTGG 1 cut(s) 103
VpaK11BI GGWCC 1 cut(s) 118
XceI RCATGY 2 cut(s) 713, 748
XmiI GTMKAC 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.