FvH4_6g18540

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001039
Physical Location & Seq
Forward (+)
12269831 .. 12271927
2097 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g18540.t1

Sequence Viewer

Length: 1074 bp
ATGCTTCTCCAGCTCGAGCAAGAGTGCCACGACATCTACCGCAAGAAGGTCGAGATGACCAGAAAGTACAGGGCCGAGTTGCACAGGTCCCTTGCCGACGGCCAAGCTCAAATCACCGCCATCGCCTCCGCTCTCGGCGAAGCCACTTCCTTTCCCCGGGCAAGAGGGACTCTCCGGGACCAGATTTCTGCTGTCAAGCCTGTTTTGGAGGAATTGAGCTTGAAGAAGCAAGAGAGGGTCAAGGACTTTTCGGTCATTCAGTCGCAGATTGCCAGGATCTCGTCGGAGATAGCCGGCAACGGTGTGGCTGGGAATGCCGGTGAGTATGCCGGAGTGGATGAGACGGATTTGACTTTGAAGAGGTTGGGAGAGCTTAAGTCTCACCTTGAGGAGCTTCACAAAGAAAAGGTACTACATTCGGTAATTGGGTTAGATATTAGTTTGTTGTTGGTTATAATTGGGTCTGATGCAGTGTGGAATGCTTTGGACTTGATCAAGATGAAGGTGTTCATCAGCCTTTCCTTCATGATATTCGGGTTCATCTTCCTTCCTCAAGAACCAAAGTTCACAATCACCAATGCTACACTAACCCAATTCAACTTCAACAGCTCCAACAACACCCTCGACTACAACCTCACACTCAACATGACCATCAGAAACCCTAACAAGAGGGTTGGCATATATTATCGCCGCATTCAGGTAATCGGTGACTATCGAAAGAAGAGGTTTGCTCTCGTGACTTTGAATACTTTGCCGTTCTACCAAGGCCACAAAAACACCACCGAATTGCATCAAGTGGTTGTTCAAGGATCGCAGCCGGTGAAGTTTGGAAAACGCGAGGTGTCCCAGTTTACCGAGGAGACTGCTTCTGGGATTTACAGTATAAAGGTGCAGCTTGCTCTTCGGATAAAAGCCAGGTACGGCAAGTTCACGTCGCGCAATTACCAACCGTATCGGAAGATTGACTGCAAGCTGAATGTTCCTTTGAGTTTTAATGAAACATCAATTGCCAGTGGTTTCAAGGCCACAAGGTGTGAAAATGTTTACTTCTTTACAGATCCTGATTCAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

358

Amino Acids

40.49

Weight (kDa)

9.4

Isoelectric Point (pI)

30.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 2 - 137 3.2e-10 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 455
AasI GACNNNNNNGTC 1 cut(s) 251
AccBSI CCGCTC 1 cut(s) 131
AccII CGCG 2 cut(s) 837, 937
AciI CCGC 4 cut(s) 40, 117, 129, 691
AclWI GGATC 3 cut(s) 284, 817, 1052
AcoI YGGCCR 1 cut(s) 100
AdeI CACNNNGTG 1 cut(s) 1032
AfaI GTAC 3 cut(s) 68, 411, 920
AfiI CCNNNNNNNGG 3 cut(s) 46, 156, 697
AflII CTTAAG 1 cut(s) 374
AgsI TTSAA 7 cut(s) 223, 358, 598, 604, 745, 806, 1021
AjiI CACGTC 1 cut(s) 933
AjnI CCWGG 2 cut(s) 272, 914
AluBI AGCT 8 cut(s) 13, 107, 219, 373, 394, 609, 895, 973
AluI AGCT 8 cut(s) 13, 107, 219, 373, 394, 609, 895, 973
Alw26I GTCTC 3 cut(s) 335, 384, 854
AlwI GGATC 3 cut(s) 284, 817, 1052
AlwNI CAGNNNCTG 1 cut(s) 1061
Ama87I CYCGRG 2 cut(s) 14, 156
AoxI GGCC 4 cut(s) 72, 100, 766, 1023
ApeKI GCWGC 2 cut(s) 814, 892
Asp700I GAANNNNTTC 1 cut(s) 506
AspLEI GCGC 1 cut(s) 939
AspS9I GGNCC 3 cut(s) 72, 87, 178
AsuC2I CCSGG 3 cut(s) 157, 158, 176
AsuHPI GGTGA 6 cut(s) 106, 332, 374, 565, 719, 832
AvaI CYCGRG 2 cut(s) 14, 156
AvaII GGWCC 2 cut(s) 87, 178
BauI CACGAG 1 cut(s) 734
BbvI GCAGC 2 cut(s) 826, 904
BccI CCATC 2 cut(s) 128, 659
BceAI ACGGC 3 cut(s) 115, 739, 937
BcgI CGANNNNNNTGC 4 cut(s) 31, 65, 845, 879
BciT130I CCWGG 2 cut(s) 274, 916
BclI TGATCA 1 cut(s) 492
BcnI CCSGG 3 cut(s) 157, 158, 176
BcoDI GTCTC 3 cut(s) 335, 384, 854
BfrI CTTAAG 1 cut(s) 374
BisI GCNGC 3 cut(s) 691, 815, 893
BlsI GCNGC 3 cut(s) 692, 816, 894
Bme1390I CCNGG 5 cut(s) 157, 158, 176, 274, 916
Bme18I GGWCC 2 cut(s) 87, 178
BmeT110I CYCGRG 2 cut(s) 14, 156
BmgBI CACGTC 1 cut(s) 933
BmgT120I GGNCC 3 cut(s) 72, 87, 178
BmiI GGNNCC 2 cut(s) 89, 179
BmrFI CCNGG 5 cut(s) 157, 158, 176, 274, 916
BmrI ACTGGG 1 cut(s) 841
BmsI GCATC 2 cut(s) 457, 799
BmuI ACTGGG 1 cut(s) 841
BplI GAGNNNNNCTC 4 cut(s) 156, 188, 715, 747
BpuEI CTTGAG 2 cut(s) 407, 537
BpuMI CCSGG 3 cut(s) 157, 158, 176
BsaJI CCNNGG 4 cut(s) 155, 156, 763, 855
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 156, 697
Bse118I RCCGGY 3 cut(s) 293, 317, 817
Bse1I ACTGG 2 cut(s) 847, 1011
BseBI CCWGG 2 cut(s) 274, 916
BseDI CCNNGG 4 cut(s) 155, 156, 763, 855
BseGI GGATG 1 cut(s) 343
BseLI CCNNNNNNNGG 3 cut(s) 46, 156, 697
BseNI ACTGG 2 cut(s) 847, 1011
BseRI GAGGAG 2 cut(s) 404, 872
BseXI GCAGC 2 cut(s) 826, 904
BseYI CCCAGC 1 cut(s) 308
BsgI GTGCAG 1 cut(s) 911
Bsh1236I CGCG 2 cut(s) 837, 937
BshFI GGCC 4 cut(s) 74, 102, 768, 1025
BsiHKCI CYCGRG 2 cut(s) 14, 156
BsiSI CCGG 6 cut(s) 157, 175, 294, 318, 330, 818
BslFI GGGAC 4 cut(s) 73, 181, 191, 829
BslI CCNNNNNNNGG 3 cut(s) 46, 156, 697
BsmAI GTCTC 3 cut(s) 335, 384, 854
BsmBI CGTCTC 1 cut(s) 335
BsmFI GGGAC 4 cut(s) 73, 181, 191, 829
BsmI GAATGC 3 cut(s) 319, 484, 693
BsnI GGCC 4 cut(s) 74, 102, 768, 1025
BsoBI CYCGRG 2 cut(s) 14, 156
Bsp143I GATC 4 cut(s) 276, 492, 809, 1057
BspACI CCGC 4 cut(s) 40, 117, 129, 691
BspANI GGCC 4 cut(s) 74, 102, 768, 1025
BspFNI CGCG 2 cut(s) 837, 937
BspHI TCATGA 1 cut(s) 525
BspLI GGNNCC 2 cut(s) 89, 179
BspPI GGATC 3 cut(s) 284, 817, 1052
BspQI GCTCTTC 1 cut(s) 906
BspTI CTTAAG 1 cut(s) 374
BsrBI CCGCTC 1 cut(s) 131
BsrFI RCCGGY 3 cut(s) 293, 317, 817
BsrI ACTGG 2 cut(s) 847, 1011
BssAI RCCGGY 3 cut(s) 293, 317, 817
BssECI CCNNGG 4 cut(s) 155, 156, 763, 855
BssMI GATC 4 cut(s) 276, 492, 809, 1057
BssSI CACGAG 1 cut(s) 734
BssT1I CCWWGG 1 cut(s) 763
Bst2BI CACGAG 1 cut(s) 734
Bst2UI CCWGG 2 cut(s) 274, 916
Bst4CI ACNGT 3 cut(s) 302, 881, 951
Bst6I CTCTTC 3 cut(s) 353, 716, 906
BstAFI CTTAAG 1 cut(s) 374
BstC8I GCNNGC 3 cut(s) 295, 897, 971
BstF5I GGATG 1 cut(s) 343
BstFNI CGCG 2 cut(s) 837, 937
BstHHI GCGC 1 cut(s) 939
BstKTI GATC 4 cut(s) 279, 495, 812, 1060
BstMAI GTCTC 3 cut(s) 335, 384, 854
BstMBI GATC 4 cut(s) 276, 492, 809, 1057
BstMWI GCNNNNNNNGC 2 cut(s) 10, 314
BstNI CCWGG 2 cut(s) 274, 916
BstSCI CCNGG 5 cut(s) 155, 156, 174, 272, 914
BstUI CGCG 2 cut(s) 837, 937
BstV1I GCAGC 2 cut(s) 826, 904
BstX2I RGATCY 2 cut(s) 276, 1057
BstYI RGATCY 2 cut(s) 276, 1057
BsuRI GGCC 4 cut(s) 74, 102, 768, 1025
BtgZI GCGATG 1 cut(s) 106
BtrI CACGTC 1 cut(s) 933
BtsCI GGATG 1 cut(s) 343
BtsI GCAGTG 1 cut(s) 477
BtsIMutI CAGTG 2 cut(s) 477, 1018
Cac8I GCNNGC 3 cut(s) 295, 897, 971
CaiI CAGNNNCTG 1 cut(s) 1061
CciI TCATGA 1 cut(s) 525
CfoI GCGC 1 cut(s) 939
Cfr10I RCCGGY 3 cut(s) 293, 317, 817
Cfr13I GGNCC 3 cut(s) 72, 87, 178
Cfr9I CCCGGG 1 cut(s) 156
Csp6I GTAC 3 cut(s) 67, 410, 919
CviAII CATG 2 cut(s) 526, 646
CviQI GTAC 3 cut(s) 67, 410, 919
DpnI GATC 4 cut(s) 278, 494, 811, 1059
DpnII GATC 4 cut(s) 276, 492, 809, 1057
DraIII CACNNNGTG 1 cut(s) 1032
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
EaeI YGGCCR 1 cut(s) 100
Eam1104I CTCTTC 3 cut(s) 353, 716, 906
EarI CTCTTC 3 cut(s) 353, 716, 906
Eco130I CCWWGG 1 cut(s) 763
Eco47I GGWCC 2 cut(s) 87, 178
Eco88I CYCGRG 2 cut(s) 14, 156
EcoO109I RGGNCCY 1 cut(s) 87
EcoRII CCWGG 2 cut(s) 272, 914
EcoT14I CCWWGG 1 cut(s) 763
ErhI CCWWGG 1 cut(s) 763
Esp3I CGTCTC 1 cut(s) 335
FaeI CATG 2 cut(s) 529, 649
FaiI YATR 7 cut(s) 327, 455, 527, 647, 680, 682, 884
FaqI GGGAC 4 cut(s) 73, 181, 191, 829
FatI CATG 2 cut(s) 525, 645
FbaI TGATCA 1 cut(s) 492
Fnu4HI GCNGC 3 cut(s) 691, 815, 893
FokI GGATG 1 cut(s) 350
Fsp4HI GCNGC 3 cut(s) 691, 815, 893
GlaI GCGC 1 cut(s) 938
GluI GCNGC 3 cut(s) 691, 815, 893
GsaI CCCAGC 1 cut(s) 312
HaeIII GGCC 4 cut(s) 74, 102, 768, 1025
HapII CCGG 6 cut(s) 157, 175, 294, 318, 330, 818
HhaI GCGC 1 cut(s) 939
Hin1II CATG 2 cut(s) 529, 649
Hin6I GCGC 1 cut(s) 937
HinP1I GCGC 1 cut(s) 937
HinfI GANTC 2 cut(s) 169, 1064
HpaII CCGG 6 cut(s) 157, 175, 294, 318, 330, 818
HphI GGTGA 6 cut(s) 106, 332, 374, 565, 719, 832
Hpy166II GTNNAC 4 cut(s) 567, 852, 930, 1045
Hpy188I TCNGA 6 cut(s) 286, 466, 656, 906, 957, 1069
Hpy188III TCNNGA 6 cut(s) 52, 496, 526, 554, 736, 1061
Hpy8I GTNNAC 4 cut(s) 567, 852, 930, 1045
Hpy99I CGWCG 3 cut(s) 101, 286, 937
HpyAV CCTTC 4 cut(s) 40, 496, 532, 557
HpyCH4III ACNGT 3 cut(s) 302, 881, 951
HpyCH4IV ACGT 1 cut(s) 932
HpyCH4V TGCA 5 cut(s) 82, 470, 790, 892, 969
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 314
HpySE526I ACGT 1 cut(s) 932
Hsp92II CATG 2 cut(s) 529, 649
HspAI GCGC 1 cut(s) 937
KroI GCCGGC 1 cut(s) 293
KroNI GCCGGC 1 cut(s) 295
Ksp22I TGATCA 1 cut(s) 492
Kzo9I GATC 4 cut(s) 276, 492, 809, 1057
LguI GCTCTTC 1 cut(s) 906
LmnI GCTCC 2 cut(s) 391, 614
Lsp1109I GCAGC 2 cut(s) 826, 904
LweI GCATC 2 cut(s) 457, 799
MaeII ACGT 1 cut(s) 932
MaeIII GTNAC 2 cut(s) 707, 736
MalI GATC 4 cut(s) 278, 494, 811, 1059
MbiI CCGCTC 1 cut(s) 131
MboI GATC 4 cut(s) 276, 492, 809, 1057
MboII GAAGA 6 cut(s) 235, 370, 535, 733, 893, 970
MfeI CAATTG 1 cut(s) 1005
MflI RGATCY 2 cut(s) 276, 1057
MluCI AATT 7 cut(s) 212, 423, 456, 593, 785, 940, 1005
MlyI GAGTC 1 cut(s) 163
MmeI TCCRAC 2 cut(s) 264, 636
MroNI GCCGGC 1 cut(s) 293
MroXI GAANNNNTTC 1 cut(s) 506
MseI TTAA 3 cut(s) 375, 993, 1072
MspCI CTTAAG 1 cut(s) 374
MspI CCGG 6 cut(s) 157, 175, 294, 318, 330, 818
MspR9I CCNGG 5 cut(s) 157, 158, 176, 274, 916
MunI CAATTG 1 cut(s) 1005
Mva1269I GAATGC 3 cut(s) 319, 484, 693
MvaI CCWGG 2 cut(s) 274, 916
MvnI CGCG 2 cut(s) 837, 937
MwoI GCNNNNNNNGC 2 cut(s) 10, 314
NaeI GCCGGC 1 cut(s) 295
NciI CCSGG 3 cut(s) 157, 158, 176
NdeII GATC 4 cut(s) 276, 492, 809, 1057
NgoMIV GCCGGC 1 cut(s) 293
NlaIII CATG 2 cut(s) 529, 649
NlaIV GGNNCC 2 cut(s) 89, 179
NmeAIII GCCGAG 2 cut(s) 100, 114
NmuCI GTSAC 2 cut(s) 707, 736
PaeR7I CTCGAG 1 cut(s) 14
PagI TCATGA 1 cut(s) 525
PciSI GCTCTTC 1 cut(s) 906
PctI GAATGC 3 cut(s) 319, 484, 693
PdiI GCCGGC 1 cut(s) 295
PdmI GAANNNNTTC 1 cut(s) 506
PfeI GAWTC 1 cut(s) 1064
PfoI TCCNGGA 1 cut(s) 174
PkrI GCNGC 3 cut(s) 692, 816, 894
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
PpuMI RGGWCCY 1 cut(s) 87
PsiI TTATAA 1 cut(s) 455
Psp5II RGGWCCY 1 cut(s) 87
Psp6I CCWGG 2 cut(s) 272, 914
PspFI CCCAGC 1 cut(s) 308
PspGI CCWGG 2 cut(s) 272, 914
PspN4I GGNNCC 2 cut(s) 89, 179
PspPI GGNCC 3 cut(s) 72, 87, 178
PspPPI RGGWCCY 1 cut(s) 87
PspXI VCTCGAGB 1 cut(s) 14
PsrI GAACNNNNNNTAC 2 cut(s) 911, 943
PstNI CAGNNNCTG 1 cut(s) 1061
PsuI RGATCY 2 cut(s) 276, 1057
RsaI GTAC 3 cut(s) 68, 411, 920
RsaNI GTAC 3 cut(s) 67, 410, 919
SapI GCTCTTC 1 cut(s) 906
SaqAI TTAA 3 cut(s) 375, 993, 1072
SatI GCNGC 3 cut(s) 691, 815, 893
Sau3AI GATC 4 cut(s) 276, 492, 809, 1057
Sau96I GGNCC 3 cut(s) 72, 87, 178
SchI GAGTC 1 cut(s) 163
ScrFI CCNGG 5 cut(s) 157, 158, 176, 274, 916
SfaNI GCATC 2 cut(s) 457, 799
Sfr274I CTCGAG 1 cut(s) 14
SinI GGWCC 2 cut(s) 87, 178
SlaI CTCGAG 1 cut(s) 14
SmaI CCCGGG 1 cut(s) 158
SmlI CTYRAG 4 cut(s) 14, 374, 386, 552
SmoI CTYRAG 4 cut(s) 14, 374, 386, 552
Sse9I AATT 7 cut(s) 212, 423, 456, 593, 785, 940, 1005
SsiI CCGC 4 cut(s) 40, 117, 129, 691
StyD4I CCNGG 5 cut(s) 155, 156, 174, 272, 914
StyI CCWWGG 1 cut(s) 763
TaaI ACNGT 3 cut(s) 302, 881, 951
TaiI ACGT 1 cut(s) 935
TaqI TCGA 4 cut(s) 15, 51, 624, 715
TaqII GACCGA 1 cut(s) 241
TasI AATT 7 cut(s) 212, 423, 456, 593, 785, 940, 1005
TatI WGTACW 1 cut(s) 66
TauI GCSGC 1 cut(s) 693
TfiI GAWTC 1 cut(s) 1064
Tru1I TTAA 3 cut(s) 375, 993, 1072
Tru9I TTAA 3 cut(s) 375, 993, 1072
TscAI CASTG 2 cut(s) 477, 1018
TseFI GTSAC 2 cut(s) 707, 736
TseI GCWGC 2 cut(s) 814, 892
Tsp45I GTSAC 2 cut(s) 707, 736
TspDTI ATGAA 5 cut(s) 499, 514, 515, 529, 1011
TspGWI ACGGA 1 cut(s) 359
TspMI CCCGGG 1 cut(s) 156
TspRI CASTG 2 cut(s) 477, 1018
Vha464I CTTAAG 1 cut(s) 374
VpaK11BI GGWCC 2 cut(s) 87, 178
XhoI CTCGAG 1 cut(s) 14
XmaI CCCGGG 1 cut(s) 156
XmnI GAANNNNTTC 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.