Rw0G014710

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00657
OG0001039
Physical Location & Seq
Forward (+)
14656 .. 16081
1426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G014710.1

Sequence Viewer

Length: 633 bp
ATGACTGAAAGGCGTGCCTGCATCGACTGCAGCTTCTGCCTATTATGCAACACCATCCTCTGCTTCACCCTAGTCTTCTTCATAGTGTGGTTCATCTTCCTTCCCCAAGAGCCCAAATTCAGCATCACTAATGCCTCTCTAACCCATTTCGACTTCATCGAGACCAACAAAACTCTCCACTACAACCTTGAGCTCAACATTACCATCACAAACCCCAATAAGAAGGTCGACATATACTACAATGGCATCCAAGTCATTGCTAACTATAAAACAAAGGAGTTTGCCATGCTGACTTTAGATTCCACACCATTTTACCAAGGCCACAAGAACACAACCATTTGGATCATGTGGTACTTGAAGGGCAGAAATCGGTGGAAGACTGCTGCTGGTTTTTACAGTATTGATGTGCAGCTTGCTCTTCGAGTAAAGCTCGGGTATGACATGTTCAAGACAAGGTATTACCAGCAGTCAGGCAACAAGATCGATTGCAAGCTGAAGGTTCCTCTGAGACATGCAAGACGTTTCAAAACTACAAAGTGTGGAAATCATTGTTTATTTAAACATTACTTCACATCTAGAGGTTACAAGGGGGAAAGAAAACTCCATATGTTGAAAATGAAACCCAGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

210

Amino Acids

25.0

Weight (kDa)

9.76

Isoelectric Point (pI)

41.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 67 - 144 4.9e-06 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 228
AclWI GGATC 1 cut(s) 350
AcsI RAATTY 1 cut(s) 116
AcuI CTGAAG 1 cut(s) 515
AfaI GTAC 1 cut(s) 353
AflIII ACRYGT 1 cut(s) 441
AgsI TTSAA 4 cut(s) 358, 448, 526, 613
AluBI AGCT 5 cut(s) 33, 193, 412, 430, 493
AluI AGCT 5 cut(s) 33, 193, 412, 430, 493
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 2 cut(s) 155, 502
AlwI GGATC 1 cut(s) 350
AlwNI CAGNNNCTG 1 cut(s) 36
Ama87I CYCGRG 1 cut(s) 431
AoxI GGCC 1 cut(s) 319
ApeKI GCWGC 3 cut(s) 30, 383, 409
ApoI RAATTY 1 cut(s) 116
AsuHPI GGTGA 1 cut(s) 58
AvaI CYCGRG 1 cut(s) 431
BanII GRGCYC 2 cut(s) 114, 195
BbsI GAAGAC 2 cut(s) 67, 383
Bbv12I GWGCWC 1 cut(s) 195
BbvI GCAGC 3 cut(s) 42, 370, 421
BccI CCATC 2 cut(s) 62, 212
BcgI CGANNNNNNTGC 2 cut(s) 463, 497
BcoDI GTCTC 2 cut(s) 155, 502
BfaI CTAG 2 cut(s) 71, 576
BfmI CTRYAG 1 cut(s) 28
BisI GCNGC 3 cut(s) 31, 384, 410
BlsI GCNGC 3 cut(s) 32, 385, 411
BmeT110I CYCGRG 1 cut(s) 431
BmiI GGNNCC 1 cut(s) 501
BmsI GCATC 3 cut(s) 30, 132, 255
BpiI GAAGAC 2 cut(s) 67, 383
BplI GAGNNNNNCTC 2 cut(s) 414, 446
BpuEI CTTGAG 1 cut(s) 209
Bsa29I ATCGAT 1 cut(s) 483
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 1 cut(s) 316
Bse3DI GCAATG 1 cut(s) 255
BseCI ATCGAT 1 cut(s) 483
BseDI CCNNGG 1 cut(s) 316
BseGI GGATG 2 cut(s) 54, 246
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 1 cut(s) 497
BseXI GCAGC 3 cut(s) 42, 370, 421
BsgI GTGCAG 1 cut(s) 428
BshFI GGCC 1 cut(s) 321
BshVI ATCGAT 1 cut(s) 483
BsiHKAI GWGCWC 1 cut(s) 195
BsiHKCI CYCGRG 1 cut(s) 431
BsmAI GTCTC 2 cut(s) 155, 502
BsnI GGCC 1 cut(s) 321
Bso31I GGTCTC 1 cut(s) 155
BsoBI CYCGRG 1 cut(s) 431
Bsp1286I GDGCHC 2 cut(s) 114, 195
Bsp143I GATC 2 cut(s) 342, 480
BspANI GGCC 1 cut(s) 321
BspCNI CTCAG 1 cut(s) 498
BspDI ATCGAT 1 cut(s) 483
BspLI GGNNCC 1 cut(s) 501
BspMAI CTGCAG 1 cut(s) 32
BspPI GGATC 1 cut(s) 350
BspQI GCTCTTC 1 cut(s) 423
BspTNI GGTCTC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 255
BssECI CCNNGG 1 cut(s) 316
BssMI GATC 2 cut(s) 342, 480
BssT1I CCWWGG 1 cut(s) 316
Bst4CI ACNGT 1 cut(s) 398
Bst6I CTCTTC 1 cut(s) 423
BstAPI GCANNNNNTGC 2 cut(s) 27, 36
BstC8I GCNNGC 4 cut(s) 15, 19, 414, 491
BstDEI CTNAG 1 cut(s) 506
BstF5I GGATG 2 cut(s) 54, 246
BstKTI GATC 2 cut(s) 345, 483
BstMAI GTCTC 2 cut(s) 155, 502
BstMBI GATC 2 cut(s) 342, 480
BstMWI GCNNNNNNNGC 3 cut(s) 27, 36, 45
BstNSI RCATGY 2 cut(s) 445, 515
BstSFI CTRYAG 1 cut(s) 28
BstV1I GCAGC 3 cut(s) 42, 370, 421
BstV2I GAAGAC 2 cut(s) 67, 383
Bsu15I ATCGAT 1 cut(s) 483
BsuRI GGCC 1 cut(s) 321
BsuTUI ATCGAT 1 cut(s) 483
BtsCI GGATG 2 cut(s) 54, 246
Cac8I GCNNGC 4 cut(s) 15, 19, 414, 491
CaiI CAGNNNCTG 1 cut(s) 36
ClaI ATCGAT 1 cut(s) 483
Csp6I GTAC 1 cut(s) 352
CviAII CATG 4 cut(s) 286, 346, 442, 512
CviJI RGCY 7 cut(s) 33, 112, 193, 321, 412, 430, 493
CviKI_1 RGCY 7 cut(s) 33, 112, 193, 321, 412, 430, 493
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 1 cut(s) 506
DpnI GATC 2 cut(s) 344, 482
DpnII GATC 2 cut(s) 342, 480
DraI TTTAAA 1 cut(s) 559
Eam1104I CTCTTC 1 cut(s) 423
EarI CTCTTC 1 cut(s) 423
Ecl136II GAGCTC 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 316
Eco24I GRGCYC 2 cut(s) 114, 195
Eco31I GGTCTC 1 cut(s) 155
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 515
Eco88I CYCGRG 1 cut(s) 431
EcoICRI GAGCTC 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 316
EcoT38I GRGCYC 2 cut(s) 114, 195
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 4 cut(s) 289, 349, 445, 515
FatI CATG 4 cut(s) 285, 345, 441, 511
FauNDI CATATG 1 cut(s) 606
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 3 cut(s) 31, 384, 410
FokI GGATG 2 cut(s) 41, 233
FriOI GRGCYC 2 cut(s) 114, 195
Fsp4HI GCNGC 3 cut(s) 31, 384, 410
FspBI CTAG 2 cut(s) 71, 576
GluI GCNGC 3 cut(s) 31, 384, 410
HaeIII GGCC 1 cut(s) 321
Hin1II CATG 4 cut(s) 289, 349, 445, 515
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 1 cut(s) 299
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 1 cut(s) 507
Hpy188III TCNNGA 3 cut(s) 160, 448, 576
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 4 cut(s) 110, 217, 352, 490
HpyCH4III ACNGT 1 cut(s) 398
HpyCH4IV ACGT 1 cut(s) 520
HpyCH4V TGCA 6 cut(s) 21, 30, 48, 409, 489, 515
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 36, 45
HpyF3I CTNAG 1 cut(s) 506
HpySE526I ACGT 1 cut(s) 520
Hsp92II CATG 4 cut(s) 289, 349, 445, 515
Kzo9I GATC 2 cut(s) 342, 480
LguI GCTCTTC 1 cut(s) 423
LpnPI CCDG 4 cut(s) 31, 372, 456, 476
Lsp1109I GCAGC 3 cut(s) 42, 370, 421
LweI GCATC 3 cut(s) 30, 132, 255
MaeI CTAG 2 cut(s) 71, 576
MaeII ACGT 1 cut(s) 520
MaeIII GTNAC 1 cut(s) 581
MalI GATC 2 cut(s) 344, 482
MboI GATC 2 cut(s) 342, 480
MboII GAAGA 5 cut(s) 67, 70, 88, 388, 410
MhlI GDGCHC 2 cut(s) 114, 195
MluCI AATT 1 cut(s) 116
MnlI CCTC 4 cut(s) 68, 145, 513, 572
MseI TTAA 1 cut(s) 558
MwoI GCNNNNNNNGC 3 cut(s) 27, 36, 45
NdeI CATATG 1 cut(s) 606
NdeII GATC 2 cut(s) 342, 480
NlaIII CATG 4 cut(s) 289, 349, 445, 515
NlaIV GGNNCC 1 cut(s) 501
NspI RCATGY 2 cut(s) 445, 515
PciI ACATGT 1 cut(s) 441
PciSI GCTCTTC 1 cut(s) 423
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PfeI GAWTC 1 cut(s) 299
PkrI GCNGC 3 cut(s) 32, 385, 411
PscI ACATGT 1 cut(s) 441
Psp124BI GAGCTC 1 cut(s) 195
PspN4I GGNNCC 1 cut(s) 501
PsrI GAACNNNNNNTAC 2 cut(s) 428, 460
PstI CTGCAG 1 cut(s) 32
PstNI CAGNNNCTG 1 cut(s) 36
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
SacI GAGCTC 1 cut(s) 195
SalI GTCGAC 1 cut(s) 227
SapI GCTCTTC 1 cut(s) 423
SaqAI TTAA 1 cut(s) 558
SatI GCNGC 3 cut(s) 31, 384, 410
Sau3AI GATC 2 cut(s) 342, 480
SduI GDGCHC 2 cut(s) 114, 195
SfaNI GCATC 3 cut(s) 30, 132, 255
SfcI CTRYAG 1 cut(s) 28
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 1 cut(s) 116
SspMI CTAG 2 cut(s) 71, 576
SstI GAGCTC 1 cut(s) 195
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 1 cut(s) 398
TaiI ACGT 1 cut(s) 523
TaqI TCGA 6 cut(s) 24, 150, 159, 228, 421, 483
TasI AATT 1 cut(s) 116
TfiI GAWTC 1 cut(s) 299
Tru1I TTAA 1 cut(s) 558
Tru9I TTAA 1 cut(s) 558
TseI GCWGC 3 cut(s) 30, 383, 409
TspDTI ATGAA 4 cut(s) 70, 82, 145, 632
XapI RAATTY 1 cut(s) 116
XbaI TCTAGA 1 cut(s) 575
XceI RCATGY 2 cut(s) 445, 515
XmiI GTMKAC 1 cut(s) 228
XspI CTAG 2 cut(s) 71, 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.