Rh3BG209500

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001039
Physical Location & Seq
Forward (+)
18900558 .. 18903531
2974 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG209500.1

Sequence Viewer

Length: 561 bp
ATGATTCCTAGAGAAGAAGACTCACCGAACCTGGATAACGTTGGTTGCAAGTATTACGTCACTGAATTAGAGCTCATCATCGAACTTTACAGTGAAAACCCAAAGCCAATTAGATCTCCCAAGCTCGAAGTAACAGTCACCAATGCGTCTATGACCCAATTCAATCTCAACAGCAACAACAACACTCTCTACTACAACCTCGCCCTCGATGTCGCCATCAGAAACCCTAGCCCAACCTTCAGGGTACACTACAATCGCATCGAAGTCGCGGCGAAATATAGGGAGCAGAAATTTGCTTTGGTGACTTTGAGTTCGCCACCCTTTGACCAAGGAACCAAGAACATAACCATTCTGCATCCCCTTCTTCAAGGGCAACCCTCTAGCCTGGAGACCGATGCAGGCATTTACAGTATTGACCTGCAGTTTTCTTTTAGGATAAGCCGCCGGGTTCTCAAAGGAATCGTTTACAAACCAGTAGGGATGAGCTTAACAGTGACCATTGTCATAGAATATGCCACGAACTTCGCCACTAATGCCTATTGGAAGCGAAAACATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

186

Amino Acids

21.28

Weight (kDa)

8.91

Isoelectric Point (pI)

49.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 71 - 123 2.3e-06 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 426
AccII CGCG 1 cut(s) 269
AciI CCGC 2 cut(s) 269, 442
AclI AACGTT 1 cut(s) 39
AcsI RAATTY 1 cut(s) 290
AcuI CTGAAG 1 cut(s) 223
AfaI GTAC 1 cut(s) 246
AgsI TTSAA 2 cut(s) 163, 368
AjnI CCWGG 2 cut(s) 30, 384
AjuI GAANNNNNNNTTGG 2 cut(s) 281, 313
AluBI AGCT 3 cut(s) 73, 124, 486
AluI AGCT 3 cut(s) 73, 124, 486
Alw21I GWGCWC 1 cut(s) 75
Alw26I GTCTC 1 cut(s) 383
ApoI RAATTY 1 cut(s) 290
ArsI GACNNNNNNTTYG 4 cut(s) 120, 152, 295, 327
AsuC2I CCSGG 1 cut(s) 446
AsuHPI GGTGA 3 cut(s) 15, 130, 313
BanII GRGCYC 1 cut(s) 75
BbsI GAAGAC 1 cut(s) 24
Bbv12I GWGCWC 1 cut(s) 75
BccI CCATC 1 cut(s) 224
BcgI CGANNNNNNTGC 2 cut(s) 247, 281
BciT130I CCWGG 2 cut(s) 32, 386
BcnI CCSGG 1 cut(s) 446
BcoDI GTCTC 1 cut(s) 383
BfaI CTAG 3 cut(s) 9, 228, 381
BfmI CTRYAG 1 cut(s) 419
BfuAI ACCTGC 1 cut(s) 426
BglII AGATCT 1 cut(s) 113
BisI GCNGC 2 cut(s) 270, 442
BlsI GCNGC 2 cut(s) 271, 443
Bme1390I CCNGG 3 cut(s) 32, 386, 446
BmiI GGNNCC 1 cut(s) 334
BmrFI CCNGG 3 cut(s) 32, 386, 446
BmsI GCATC 3 cut(s) 267, 364, 385
BoxI GACNNNNGTC 1 cut(s) 500
BpiI GAAGAC 1 cut(s) 24
BpmI CTGGAG 1 cut(s) 407
BpuMI CCSGG 1 cut(s) 446
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 1 cut(s) 328
Bse1I ACTGG 1 cut(s) 473
BseBI CCWGG 2 cut(s) 32, 386
BseDI CCNNGG 1 cut(s) 328
BseGI GGATG 2 cut(s) 355, 486
BseNI ACTGG 1 cut(s) 473
Bsh1236I CGCG 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 75
BsiSI CCGG 1 cut(s) 445
BsmAI GTCTC 1 cut(s) 383
Bso31I GGTCTC 1 cut(s) 383
Bsp1286I GDGCHC 1 cut(s) 75
Bsp143I GATC 1 cut(s) 113
BspACI CCGC 2 cut(s) 269, 442
BspFNI CGCG 1 cut(s) 269
BspLI GGNNCC 1 cut(s) 334
BspMAI CTGCAG 1 cut(s) 423
BspMI ACCTGC 1 cut(s) 426
BspTNI GGTCTC 1 cut(s) 383
BsrI ACTGG 1 cut(s) 473
BssECI CCNNGG 1 cut(s) 328
BssMI GATC 1 cut(s) 113
BssT1I CCWWGG 1 cut(s) 328
Bst2UI CCWGG 2 cut(s) 32, 386
Bst4CI ACNGT 4 cut(s) 92, 136, 410, 493
BstC8I GCNNGC 1 cut(s) 400
BstF5I GGATG 2 cut(s) 355, 486
BstFNI CGCG 1 cut(s) 269
BstKTI GATC 1 cut(s) 116
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 1 cut(s) 113
BstMWI GCNNNNNNNGC 1 cut(s) 533
BstNI CCWGG 2 cut(s) 32, 386
BstPAI GACNNNNGTC 1 cut(s) 500
BstSCI CCNGG 3 cut(s) 30, 384, 444
BstSFI CTRYAG 1 cut(s) 419
BstUI CGCG 1 cut(s) 269
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 113
BstYI RGATCY 1 cut(s) 113
BtsCI GGATG 2 cut(s) 355, 486
BtsIMutI CAGTG 3 cut(s) 60, 97, 498
BveI ACCTGC 1 cut(s) 426
Cac8I GCNNGC 1 cut(s) 400
CseI GACGC 1 cut(s) 135
Csp6I GTAC 1 cut(s) 245
CviJI RGCY 7 cut(s) 73, 106, 124, 231, 384, 441, 486
CviKI_1 RGCY 7 cut(s) 73, 106, 124, 231, 384, 441, 486
CviQI GTAC 1 cut(s) 245
DpnI GATC 1 cut(s) 115
DpnII GATC 1 cut(s) 113
Ecl136II GAGCTC 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 328
Eco24I GRGCYC 1 cut(s) 75
Eco31I GGTCTC 1 cut(s) 383
Eco53kI GAGCTC 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 223
EcoICRI GAGCTC 1 cut(s) 73
EcoRII CCWGG 2 cut(s) 30, 384
EcoT14I CCWWGG 1 cut(s) 328
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 328
FaiI YATR 5 cut(s) 152, 279, 344, 506, 513
Fnu4HI GCNGC 2 cut(s) 270, 442
FokI GGATG 2 cut(s) 342, 493
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 2 cut(s) 270, 442
FspBI CTAG 3 cut(s) 9, 228, 381
GluI GCNGC 2 cut(s) 270, 442
GsuI CTGGAG 1 cut(s) 407
HapII CCGG 1 cut(s) 445
HgaI GACGC 1 cut(s) 135
HinfI GANTC 3 cut(s) 4, 20, 459
HpaII CCGG 1 cut(s) 445
HphI GGTGA 3 cut(s) 15, 130, 313
Hpy166II GTNNAC 2 cut(s) 247, 466
Hpy188I TCNGA 1 cut(s) 221
Hpy8I GTNNAC 2 cut(s) 247, 466
HpyAV CCTTC 2 cut(s) 247, 371
HpyCH4III ACNGT 4 cut(s) 92, 136, 410, 493
HpyCH4IV ACGT 2 cut(s) 39, 57
HpyCH4V TGCA 4 cut(s) 48, 355, 398, 421
HpyF10VI GCNNNNNNNGC 1 cut(s) 533
HpySE526I ACGT 2 cut(s) 39, 57
Kzo9I GATC 1 cut(s) 113
LmnI GCTCC 1 cut(s) 283
LpnPI CCDG 9 cut(s) 17, 44, 226, 371, 384, 398, 431, 458, 486
LweI GCATC 3 cut(s) 267, 364, 385
MaeI CTAG 3 cut(s) 9, 228, 381
MaeII ACGT 2 cut(s) 39, 57
MaeIII GTNAC 5 cut(s) 58, 130, 136, 301, 493
MalI GATC 1 cut(s) 115
MboI GATC 1 cut(s) 113
MboII GAAGA 3 cut(s) 26, 29, 356
MflI RGATCY 1 cut(s) 113
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 4 cut(s) 65, 108, 158, 290
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 3 cut(s) 209, 215, 388
MseI TTAA 2 cut(s) 488, 559
MspI CCGG 1 cut(s) 445
MspR9I CCNGG 3 cut(s) 32, 386, 446
MvaI CCWGG 2 cut(s) 32, 386
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 1 cut(s) 533
NciI CCSGG 1 cut(s) 446
NdeII GATC 1 cut(s) 113
NlaIV GGNNCC 1 cut(s) 334
NmuCI GTSAC 4 cut(s) 58, 136, 301, 493
PfeI GAWTC 2 cut(s) 4, 459
PkrI GCNGC 2 cut(s) 271, 443
PleI GAGTC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 14
PshAI GACNNNNGTC 1 cut(s) 500
Psp124BI GAGCTC 1 cut(s) 75
Psp1406I AACGTT 1 cut(s) 39
Psp6I CCWGG 2 cut(s) 30, 384
PspGI CCWGG 2 cut(s) 30, 384
PspN4I GGNNCC 1 cut(s) 334
PstI CTGCAG 1 cut(s) 423
PsuI RGATCY 1 cut(s) 113
RsaI GTAC 1 cut(s) 246
RsaNI GTAC 1 cut(s) 245
SacI GAGCTC 1 cut(s) 75
SaqAI TTAA 2 cut(s) 488, 559
SatI GCNGC 2 cut(s) 270, 442
Sau3AI GATC 1 cut(s) 113
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 3 cut(s) 32, 386, 446
SduI GDGCHC 1 cut(s) 75
SetI ASST 9 cut(s) 33, 42, 60, 75, 126, 201, 239, 420, 488
SfaNI GCATC 3 cut(s) 267, 364, 385
SfcI CTRYAG 1 cut(s) 419
Sse9I AATT 4 cut(s) 65, 108, 158, 290
SsiI CCGC 2 cut(s) 269, 442
SspMI CTAG 3 cut(s) 9, 228, 381
SstI GAGCTC 1 cut(s) 75
StyD4I CCNGG 3 cut(s) 30, 384, 444
StyI CCWWGG 1 cut(s) 328
TaaI ACNGT 4 cut(s) 92, 136, 410, 493
TaiI ACGT 2 cut(s) 42, 60
TaqI TCGA 4 cut(s) 81, 126, 207, 261
TaqII GACCGA 1 cut(s) 407
TasI AATT 4 cut(s) 65, 108, 158, 290
TauI GCSGC 2 cut(s) 272, 444
TfiI GAWTC 2 cut(s) 4, 459
Tru1I TTAA 2 cut(s) 488, 559
Tru9I TTAA 2 cut(s) 488, 559
TscAI CASTG 3 cut(s) 67, 97, 498
TseFI GTSAC 4 cut(s) 58, 136, 301, 493
Tsp45I GTSAC 4 cut(s) 58, 136, 301, 493
TspRI CASTG 3 cut(s) 67, 97, 498
XapI RAATTY 1 cut(s) 290
XspI CTAG 3 cut(s) 9, 228, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.